##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938752_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 843940 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.266293812356327 31.0 31.0 34.0 28.0 34.0 2 31.30630850534398 33.0 31.0 34.0 28.0 34.0 3 31.29232172903287 33.0 31.0 34.0 27.0 34.0 4 34.87790008768396 37.0 35.0 37.0 32.0 37.0 5 34.9209706851198 37.0 35.0 37.0 32.0 37.0 6 34.98932981017608 37.0 35.0 37.0 32.0 37.0 7 34.90035784534446 37.0 35.0 37.0 32.0 37.0 8 34.87883143351423 37.0 35.0 37.0 32.0 37.0 9 36.38517193165391 39.0 37.0 39.0 32.0 39.0 10-11 36.269186198070955 39.0 37.0 39.0 32.0 39.0 12-13 36.157639761120464 39.0 36.0 39.0 32.0 39.0 14-15 37.21921167381568 40.0 37.0 41.0 31.0 41.0 16-17 37.100488778823134 40.0 37.0 41.0 31.0 41.0 18-19 37.07880773514705 40.0 37.0 41.0 31.0 41.0 20-21 36.99692158210299 40.0 36.0 41.0 31.0 41.0 22-23 36.93013780600516 40.0 36.0 41.0 30.5 41.0 24-25 36.834495343270845 40.0 36.0 41.0 30.0 41.0 26-27 36.67440754082044 39.0 36.0 41.0 30.0 41.0 28-29 36.46972296608763 39.0 36.0 41.0 30.0 41.0 30-31 36.16641763632486 39.0 35.0 41.0 29.5 41.0 32-33 35.88988316704979 39.0 35.0 41.0 29.0 41.0 34-35 35.53170782283101 38.0 35.0 40.5 27.0 41.0 36-37 35.261949901651775 38.0 35.0 40.0 25.5 41.0 38-39 35.04295151314075 38.0 35.0 40.0 25.0 41.0 40-41 34.841153991989955 38.0 34.0 40.0 24.5 41.0 42-43 34.71820923288385 38.0 34.5 40.0 24.0 41.0 44-45 34.419582553262074 38.0 34.0 40.0 22.5 41.0 46-47 33.95906936512074 38.0 33.5 40.0 17.0 41.0 48-49 33.546912102756124 38.0 33.0 40.0 11.5 41.0 50-51 32.37431274735171 37.0 31.5 39.0 8.5 40.5 52-53 32.42281915776003 37.5 32.0 39.5 7.5 40.5 54-55 32.885616276038576 38.0 33.0 40.0 4.5 41.0 56-57 32.77530748631419 38.0 33.0 40.0 2.0 41.0 58-59 32.55704552456336 37.5 32.0 40.0 2.0 41.0 60-61 32.24394328980733 37.0 32.0 40.0 2.0 41.0 62-63 31.836894210488897 36.5 31.0 40.0 2.0 41.0 64-65 31.413260421356966 36.0 31.0 39.0 2.0 41.0 66-67 30.978041685427876 35.5 30.5 39.0 2.0 41.0 68-69 30.504058937839183 35.0 29.5 39.0 2.0 40.0 70-71 30.02386721804868 35.0 29.0 38.0 2.0 40.0 72-73 29.545185084247695 35.0 29.0 37.5 2.0 40.0 74-75 29.00909069365121 35.0 28.0 37.0 2.0 39.0 76-77 28.481076853804772 34.0 27.0 36.0 2.0 39.0 78-79 27.955122402066497 34.0 26.0 36.0 2.0 38.5 80-81 27.40341315733346 34.0 25.5 35.0 2.0 37.0 82-83 26.923125459155862 34.0 25.0 35.0 2.0 37.0 84-85 26.49698082802095 34.0 23.5 35.0 2.0 36.5 86-87 25.974632082849492 33.0 21.0 35.0 2.0 36.0 88-89 25.422530630139583 33.0 18.5 35.0 2.0 35.5 90-91 24.86054518093703 33.0 10.0 35.0 2.0 35.0 92-93 24.28944711709363 32.0 2.0 35.0 2.0 35.0 94-95 23.67340450742944 32.0 2.0 35.0 2.0 35.0 96-97 22.981535417209756 31.5 2.0 35.0 2.0 35.0 98-99 22.155264592269592 31.0 2.0 34.0 2.0 35.0 100-101 19.970361044624028 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4352970318662237 1101 2 0.3203005439456703 1101 3 0.4219739471662187 1101 4 0.025399860221519077 1101 5 0.2645156892665952 1101 6 0.4083712280710827 1101 7 0.5104252884031624 1101 8 0.5085165452671845 1101 9 0.7090150858098667 1101 10-11 0.5112165354870584 1101 12-13 0.5144880456881538 1101 14-15 0.570546893158344 1101 16-17 0.6256931710364242 1101 18-19 0.8595918620148524 1101 20-21 0.7573914290369146 1101 22-23 0.6331087960225688 1101 24-25 0.7119043717480054 1101 26-27 0.7431802366639673 1101 28-29 0.701923968677292 1101 30-31 0.8276704094924909 1101 32-33 0.6735684827587747 1101 34-35 0.6988671732842775 1101 36-37 0.5852808325167658 1101 38-39 0.5335042775567658 1101 40-41 0.7219756058774962 1101 42-43 0.8646007239541618 1101 44-45 0.6141216714646447 1101 46-47 0.43029500057992465 1101 48-49 0.7423258261179058 1101 50-51 0.6534188272402659 1101 52-53 0.6613847197716893 1101 54-55 0.5992531790817353 1101 56-57 0.6018795040221221 1101 58-59 0.39300405481851897 1101 60-61 0.41563596508333234 1101 62-63 0.4271565426820807 1101 64-65 0.26798516616395673 1101 66-67 0.006719520553584601 1101 68-69 -0.09402867508038426 1101 70-71 -0.029379864469330386 1101 72-73 -0.061723305846506094 1101 74-75 -0.35012156496851077 1101 76-77 -0.28333977451356773 1101 78-79 -0.49680006463142234 1101 80-81 -0.48960827444748745 1101 82-83 -0.47878007646989573 1101 84-85 -0.5549561183378486 1101 86-87 -1.1154453326898768 1101 88-89 -1.1317187737432768 1101 90-91 -1.2540562728459577 1101 92-93 -1.322946559964027 1101 94-95 -1.468542320734759 1101 96-97 -1.4316601011137138 1101 98-99 -1.0483276894562366 1101 100-101 -0.9954954119719766 1102 1 0.31145634514906106 1102 2 0.2335791297162615 1102 3 0.111920722336464 1102 4 0.3774805154924721 1102 5 0.2853275538354296 1102 6 0.43932874382074516 1102 7 0.5449842447290578 1102 8 0.4270751191392108 1102 9 0.6228461049431857 1102 10-11 0.5567232337415859 1102 12-13 0.5708150012491444 1102 14-15 0.6264807711343181 1102 16-17 0.7205364180697558 1102 18-19 0.8120241623686155 1102 20-21 0.6490826187871548 1102 22-23 0.6425320343444483 1102 24-25 0.6702806426103294 1102 26-27 0.8399251293112897 1102 28-29 0.6413581494292373 1102 30-31 0.8339012204009251 1102 32-33 0.5460148064209918 1102 34-35 0.8938230317338522 1102 36-37 1.1098109985654858 1102 38-39 1.0737947300209427 1102 40-41 1.047576642752425 1102 42-43 1.2043738013267031 1102 44-45 1.050995536054728 1102 46-47 1.1942306633405977 1102 48-49 1.1093965482120467 1102 50-51 0.8096034249731687 1102 52-53 0.7418328282186124 1102 54-55 0.6750693462565636 1102 56-57 0.5754689397964299 1102 58-59 0.6875360268993873 1102 60-61 0.708043193461414 1102 62-63 0.3104178117914991 1102 64-65 0.1753304051445035 1102 66-67 0.008414003656557867 1102 68-69 -0.16477201666480568 1102 70-71 -0.047052420217287505 1102 72-73 0.06353564729226946 1102 74-75 -0.20103361120293428 1102 76-77 -0.1828234617937916 1102 78-79 -0.1758893994804076 1102 80-81 -0.12909769853570907 1102 82-83 0.006394524343878771 1102 84-85 0.03216368533583136 1102 86-87 -0.03938481998921972 1102 88-89 0.021448687931655996 1102 90-91 -0.3203960831063455 1102 92-93 -0.28402656718816033 1102 94-95 -0.12421166811973094 1102 96-97 -0.32305382674555716 1102 98-99 -0.6277452972073014 1102 100-101 -0.48213077408141913 1103 1 0.220764749361809 1103 2 0.3455132650725332 1103 3 0.3617440559252181 1103 4 0.40718459570612 1103 5 0.49896620078357046 1103 6 0.316350474498833 1103 7 0.5354258114266557 1103 8 0.46198837522178593 1103 9 0.4782137839296752 1103 10-11 0.47894673307039426 1103 12-13 0.7072894491843442 1103 14-15 0.5911652264767397 1103 16-17 0.5357951456742711 1103 18-19 0.6505460959647564 1103 20-21 0.6500468342605785 1103 22-23 0.7988952888661132 1103 24-25 0.8128277323682269 1103 26-27 0.8388367506974319 1103 28-29 0.786585034509514 1103 30-31 0.8891069906522873 1103 32-33 0.8476546112791041 1103 34-35 0.8954477951143787 1103 36-37 0.9798505037292529 1103 38-39 0.9478464783798586 1103 40-41 1.1337990749877278 1103 42-43 1.3413598014004933 1103 44-45 1.2143658487188063 1103 46-47 1.3652047267248193 1103 48-49 1.0128522567365934 1103 50-51 1.1345653246266423 1103 52-53 0.9611773035974167 1103 54-55 0.9255510992414457 1103 56-57 0.8246083118573111 1103 58-59 1.0253349762663717 1103 60-61 0.9613310334200875 1103 62-63 0.6923010615453045 1103 64-65 0.5423722573463792 1103 66-67 0.7420322736595928 1103 68-69 0.8843540167272756 1103 70-71 1.0349236326151896 1103 72-73 1.1117889911830154 1103 74-75 0.9555737125776425 1103 76-77 0.9580385567725038 1103 78-79 0.8216599748792675 1103 80-81 0.9972287185559523 1103 82-83 0.917574599210706 1103 84-85 0.7029774008929763 1103 86-87 0.582973343134185 1103 88-89 0.7858728973317319 1103 90-91 0.7506195571923229 1103 92-93 0.5315400850046323 1103 94-95 0.3660014383994721 1103 96-97 0.7017631922606284 1103 98-99 0.5321626375978248 1103 100-101 0.3652301561062572 1104 1 0.20253004842590627 1104 2 0.332153929789051 1104 3 0.16500846742274788 1104 4 0.36178257944379055 1104 5 0.3058725520932484 1104 6 0.44712833839417954 1104 7 0.31416477240428975 1104 8 0.4468400168726916 1104 9 0.449524132205795 1104 10-11 0.3015591234441146 1104 12-13 0.4211027074009337 1104 14-15 0.43758217997046955 1104 16-17 0.6868891166109776 1104 18-19 0.4057159281466127 1104 20-21 0.5236811068310203 1104 22-23 0.4805144658599403 1104 24-25 0.3532967397660016 1104 26-27 0.4471226499176524 1104 28-29 0.32236827380801003 1104 30-31 0.4806673298999513 1104 32-33 0.5545451280678577 1104 34-35 0.6370881403624935 1104 36-37 0.7484227299795592 1104 38-39 0.7655203039167731 1104 40-41 0.8851880680511002 1104 42-43 0.8971355123565914 1104 44-45 1.075790177765704 1104 46-47 1.4058001324913576 1104 48-49 1.5104472152473676 1104 50-51 1.6183370241707777 1104 52-53 1.506966848924428 1104 54-55 1.5052542992036635 1104 56-57 1.2596770531240153 1104 58-59 1.4764271415120973 1104 60-61 1.2229638503257974 1104 62-63 1.4527678187743156 1104 64-65 1.264575033665711 1104 66-67 0.9949040168181078 1104 68-69 1.0193183057156148 1104 70-71 0.8099331472176736 1104 72-73 0.4985263298167517 1104 74-75 0.805729140901434 1104 76-77 0.48933118149713195 1104 78-79 0.6359321564039924 1104 80-81 0.6614833987680804 1104 82-83 0.8039132968352511 1104 84-85 0.7722569024111472 1104 86-87 0.84864465737164 1104 88-89 0.9242464697941095 1104 90-91 0.7550740579465476 1104 92-93 0.8267434752589047 1104 94-95 0.8090763356834358 1104 96-97 0.5635875513097766 1104 98-99 0.5878904282932389 1104 100-101 1.0417427001026134 1105 1 0.16064722149960176 1105 2 0.1643258283957323 1105 3 0.2922040356617934 1105 4 0.23976142785741672 1105 5 0.5041414794056536 1105 6 0.49931433805843994 1105 7 0.3979398269619523 1105 8 0.37288064134809673 1105 9 0.3186636319539886 1105 10-11 0.44414700324939105 1105 12-13 0.45602028336198686 1105 14-15 0.5341878539674454 1105 16-17 0.6937032848164577 1105 18-19 0.6027372476027182 1105 20-21 0.6694571686070816 1105 22-23 0.5630506417874201 1105 24-25 0.6372242200413112 1105 26-27 0.5308110836930382 1105 28-29 0.3123209345432869 1105 30-31 0.5503910163192884 1105 32-33 0.7147062830116298 1105 34-35 0.6289202706294077 1105 36-37 0.521700554387813 1105 38-39 0.5382172990485472 1105 40-41 0.6556342658016447 1105 42-43 0.2675286632714773 1105 44-45 0.3710723667870184 1105 46-47 0.48254278819053553 1105 48-49 0.8092284809851549 1105 50-51 1.012508587037992 1105 52-53 0.7671293468260529 1105 54-55 0.8664643042397628 1105 56-57 0.9681652183078313 1105 58-59 0.7697929296604897 1105 60-61 1.1369507564866197 1105 62-63 1.1334574276378326 1105 64-65 0.8955705011829203 1105 66-67 0.962517244953073 1105 68-69 0.9118474311143068 1105 70-71 0.8312573036722632 1105 72-73 0.909341169501154 1105 74-75 0.8331704704297174 1105 76-77 0.8463289991906002 1105 78-79 0.6393566989589665 1105 80-81 0.8419467192314016 1105 82-83 0.8295637920509655 1105 84-85 0.6972057021425542 1105 86-87 0.42023102634931675 1105 88-89 0.5421977875715136 1105 90-91 0.43334701413254706 1105 92-93 0.36258567770979866 1105 94-95 0.16134895612997013 1105 96-97 0.10884053099753999 1105 98-99 0.20960404252859277 1105 100-101 0.2579508585716397 1106 1 0.03509195323844949 1106 2 0.17967295186050336 1106 3 0.35268625932048536 1106 4 0.276594007042938 1106 5 0.45411045160992103 1106 6 0.3299465293965156 1106 7 0.3064349788688645 1106 8 0.18667896067582745 1106 9 0.43347035464306316 1106 10-11 0.43644435295850315 1106 12-13 0.09581730986489845 1106 14-15 0.5482764124677715 1106 16-17 0.4696360579256975 1106 18-19 0.25658275503646166 1106 20-21 0.4375095280123844 1106 22-23 0.40753803674540734 1106 24-25 0.26581116251060166 1106 26-27 0.3200017753543207 1106 28-29 0.1256480192233127 1106 30-31 -0.006735034100877613 1106 32-33 0.21709477687070233 1106 34-35 0.3830417961627006 1106 36-37 0.5873366242003542 1106 38-39 0.3995288698307107 1106 40-41 0.8000699477667368 1106 42-43 0.742304358164823 1106 44-45 0.9117052045375118 1106 46-47 0.781669485023123 1106 48-49 0.8967268649541325 1106 50-51 0.4831420712008878 1106 52-53 0.32718235264376716 1106 54-55 0.09103573281118571 1106 56-57 0.639371423866983 1106 58-59 0.6845925418130392 1106 60-61 0.7110328508628285 1106 62-63 0.4987431419235513 1106 64-65 0.6251988140465308 1106 66-67 0.6595359909129961 1106 68-69 0.6786922921356364 1106 70-71 0.5699366831163495 1106 72-73 0.5351627596756856 1106 74-75 0.5295163010696662 1106 76-77 0.7396993935021747 1106 78-79 0.785106537355869 1106 80-81 0.44138434043372854 1106 82-83 0.4202716136411375 1106 84-85 0.7386924506881272 1106 86-87 1.0528328363040664 1106 88-89 1.2842268761300133 1106 90-91 1.2564000199502594 1106 92-93 1.224930861937338 1106 94-95 1.31581049459151 1106 96-97 1.3524817721093711 1106 98-99 1.4940688130521842 1106 100-101 1.8928461397610405 1107 1 -0.024679498077119888 1107 2 0.035180325750232555 1107 3 0.1391524483602069 1107 4 0.17235402045001535 1107 5 0.16521423072840946 1107 6 -0.23706264606854432 1107 7 -0.22644377092164802 1107 8 -0.20677147505401905 1107 9 -0.21379271725236038 1107 10-11 -0.023072573470187763 1107 12-13 -0.1571338171671144 1107 14-15 0.008275155554748892 1107 16-17 0.20326214725031377 1107 18-19 0.13218764442811448 1107 20-21 -0.08344164091672468 1107 22-23 0.025525509512284827 1107 24-25 0.00690279146987649 1107 26-27 0.04455976094171632 1107 28-29 0.03765625200360034 1107 30-31 0.11006098986426593 1107 32-33 0.15706408724442866 1107 34-35 0.023907704491847426 1107 36-37 0.2093619300491909 1107 38-39 0.1375751032813568 1107 40-41 0.35863162029900053 1107 42-43 0.12906173205454508 1107 44-45 0.6404764673161196 1107 46-47 0.8240798252275781 1107 48-49 0.7374262323079108 1107 50-51 0.72702247592688 1107 52-53 0.6263171775138758 1107 54-55 0.7744848068852619 1107 56-57 1.1409006680432867 1107 58-59 0.9243696492372138 1107 60-61 1.1229494337352932 1107 62-63 0.9617061048865132 1107 64-65 0.7864005276379551 1107 66-67 0.736011292572126 1107 68-69 0.7712482247650989 1107 70-71 0.8090198698098341 1107 72-73 0.7481414340514192 1107 74-75 0.8142961318053814 1107 76-77 0.6194599515715566 1107 78-79 0.49226675186902114 1107 80-81 0.40175492028960136 1107 82-83 0.3878144534266248 1107 84-85 0.31842263335948573 1107 86-87 0.04074028560857457 1107 88-89 0.2019854859842134 1107 90-91 0.519076511487043 1107 92-93 0.756754328503451 1107 94-95 0.5319785857595996 1107 96-97 0.6318385468705543 1107 98-99 0.7435372435867897 1107 100-101 1.2906512553970373 1108 1 0.04147607581360191 1108 2 0.1346256869530187 1108 3 -0.014484790363667344 1108 4 0.25302875459009044 1108 5 0.2856648174537497 1108 6 0.33984191938800024 1108 7 0.2376880589280006 1108 8 0.2805199624230639 1108 9 0.15216575359472273 1108 10-11 0.17210527764824946 1108 12-13 0.2908802550734464 1108 14-15 0.2754643744766412 1108 16-17 0.39530583078533965 1108 18-19 0.5097832164853173 1108 20-21 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92-93 -0.01227689583070557 2314 94-95 0.15116099414475315 2314 96-97 0.2424528405464912 2314 98-99 0.22674750308071268 2314 100-101 0.34346239877378437 2315 1 -0.5005727609719166 2315 2 -0.40902269629021504 2315 3 -0.36559789247142405 2315 4 -0.5316905148828681 2315 5 -0.33980593514298363 2315 6 -0.2767908328442772 2315 7 -0.24119894020135746 2315 8 -0.1667734632385205 2315 9 -0.39351749571179795 2315 10-11 -0.78583955824638 2315 12-13 -0.5442297896733592 2315 14-15 -0.8033727653723091 2315 16-17 -1.0094629436381197 2315 18-19 -0.8608572601390279 2315 20-21 -0.5742711398942291 2315 22-23 -0.6480074943504732 2315 24-25 -0.4438257361374838 2315 26-27 -0.2595330588719591 2315 28-29 -0.5670749170488847 2315 30-31 -0.3040103859594083 2315 32-33 -0.38231971757266336 2315 34-35 -0.6673449937585545 2315 36-37 -0.7738805148496937 2315 38-39 -0.8657657567504558 2315 40-41 -0.9856225264858196 2315 42-43 -0.6802358707629921 2315 44-45 -0.8658963740835546 2315 46-47 -0.8224919766356393 2315 48-49 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2316 7 -0.9285449105227883 2316 8 -1.16412546365801 2316 9 -1.498782033491679 2316 10-11 -1.4443688697664854 2316 12-13 -1.8245572502155483 2316 14-15 -2.1284769811973874 2316 16-17 -2.123438351325184 2316 18-19 -2.3636723983069317 2316 20-21 -2.4607119400620263 2316 22-23 -2.424884034647256 2316 24-25 -2.529154900837817 2316 26-27 -2.2319715780896203 2316 28-29 -1.9365691753468184 2316 30-31 -1.7721950642667892 2316 32-33 -2.1270794312740904 2316 34-35 -2.174053477844339 2316 36-37 -2.210865990178732 2316 38-39 -1.9991325178012644 2316 40-41 -2.1597088355414513 2316 42-43 -2.3489475272047073 2316 44-45 -2.607965484250826 2316 46-47 -2.672893109245358 2316 48-49 -2.3058875141308377 2316 50-51 -2.408794831765423 2316 52-53 -2.6580489193522148 2316 54-55 -2.290908711633257 2316 56-57 -2.0441064544638365 2316 58-59 -1.9363853365177768 2316 60-61 -1.7457293411934813 2316 62-63 -2.146556003375597 2316 64-65 -2.5044203413079202 2316 66-67 -2.1474021689663374 2316 68-69 -2.180407758140884 2316 70-71 -1.9329227940078333 2316 72-73 -2.016370674210691 2316 74-75 -2.211968113208865 2316 76-77 -2.1756789373173326 2316 78-79 -1.9352617381594683 2316 80-81 -1.6955563332716537 2316 82-83 -1.6717029967158261 2316 84-85 -1.5542625518257012 2316 86-87 -1.688871537753247 2316 88-89 -1.5014066080328838 2316 90-91 -2.2122714229858893 2316 92-93 -2.059885628761503 2316 94-95 -1.6179501891691785 2316 96-97 -1.8960727779157693 2316 98-99 -2.1407665313419812 2316 100-101 -2.446394684773903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6760.0 3 1365.0 4 1145.0 5 1270.0 6 1573.0 7 1974.0 8 2384.0 9 2790.0 10 3444.0 11 4421.0 12 5768.0 13 7242.0 14 7934.0 15 9181.0 16 10563.0 17 13219.0 18 11636.0 19 8006.0 20 8159.0 21 8714.0 22 9327.0 23 10251.0 24 11581.0 25 12970.0 26 14428.0 27 16289.0 28 18653.0 29 21609.0 30 24737.0 31 28535.0 32 33990.0 33 41129.0 34 52200.0 35 69979.0 36 93957.0 37 130744.0 38 117784.0 39 18229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62881380096949 20.206254158349967 14.887843360897254 21.27708867978329 2 15.941925782450744 22.195360186665432 39.364117463448444 22.49859656743538 3 18.485443532397966 27.09668457781376 31.846858505103846 22.571013384684427 4 12.807262619561078 18.285204276010877 37.91226985345868 30.99526325096937 5 12.731479556870537 38.07091164135352 34.232427983148064 14.965180818627877 6 28.501216250387312 36.8854492766011 19.116203256868044 15.497131216143542 7 25.24547561159564 35.23268368060793 22.568672002774555 16.953168705021874 8 24.99216036565955 33.76970005083884 21.903700782538046 19.334438800963564 9 26.56502716807659 16.62313683003903 22.468990182943976 34.342845818940404 10-11 23.51571919696949 27.5359178471079 28.366862198117033 20.581500757805575 12-13 25.097364508974156 26.114408047532876 28.660964906031378 20.127262537461586 14-15 22.496737526193257 26.84403209122939 28.41108772271317 22.248142659864182 16-17 20.09796145352267 29.886085944420778 29.029279708824905 20.98667289323164 18-19 19.862404851101882 27.738973000544515 32.65462085624656 19.744001292107043 20-21 21.334339312617757 26.86398028248943 32.391840407837144 19.40983999705567 22-23 20.987798026636955 27.126021334745985 31.985608964346675 19.90057167427038 24-25 20.365972926126695 27.298330996716864 32.471130762045746 19.864565315110696 26-27 20.133317322096932 27.47605448259135 32.505088719687635 19.885539475624082 28-29 19.891005259560814 27.650075773333732 32.46807119721867 19.990847769886784 30-31 20.196906184363367 27.566865810871434 32.283226433199545 19.953001571565654 32-33 19.93808097313538 27.7250871181477 32.02042283240374 20.31640907631318 34-35 20.39027006393513 27.584086913246697 31.985223511336546 20.040419511481627 36-37 20.460704768249737 27.376629865803746 31.71766441420006 20.445000951746454 38-39 20.176683888211222 27.53534064342006 31.908962716654543 20.379012751714175 40-41 20.261073818720337 27.464711278419884 31.913203686209652 20.36101121665013 42-43 20.111317872055217 27.631607900369374 31.46885710360626 20.788217123969144 44-45 20.075772895428646 27.748046201244247 31.253107640334495 20.923073262992613 46-47 20.191298823966065 27.933958426022638 30.73997587500684 21.13476687500446 48-49 20.354852027989335 28.03329831295369 30.338039866121413 21.27380979293556 50-51 20.19421068004076 28.31783022745754 30.07820656121342 21.409752531288277 52-53 20.25114050674251 28.46510903000563 29.886838650344338 21.396911812907522 54-55 20.080218325182226 28.18705157504155 30.063372833789963 21.669357265986264 56-57 20.28805027855882 28.422812326049414 29.67870583141839 21.610431563973375 58-59 20.258985106572727 27.931686435387498 29.908796337584352 21.900532120455424 60-61 20.207870107037593 27.964514562616312 30.103542778484073 21.72407255186203 62-63 20.277082579982107 27.71087490246084 30.079173248577355 21.932869268979694 64-65 20.107830374800393 27.705992665345065 30.047572924128445 22.1386040357261 66-67 20.041221017959227 27.873214430212244 29.64402842631862 22.44153612550991 68-69 20.035856092471352 27.687734253159725 29.73836238930339 22.53804726506554 70-71 19.870869929211114 27.68109120919464 29.712887599955017 22.73515126163923 72-73 19.984840362712767 27.408719409273914 29.689209586745193 22.917230641268127 74-75 19.753304562312817 27.519635805047184 29.585009560593463 23.142050072046537 76-77 19.768591442725462 27.319915103004234 29.710795814159336 23.200697640110977 78-79 19.754869019654485 27.196455651036246 29.705212452796527 23.343462876512742 80-81 19.646557405129695 27.228568981555274 29.615701211171036 23.509172402143992 82-83 19.733060354365087 27.020278324343742 29.560957504264803 23.68570381702637 84-85 19.571398320711307 26.622305179432637 29.670328363038518 24.13596813681754 86-87 19.655615431283678 26.417448818148294 29.718365987414202 24.208569763153825 88-89 19.946476126913083 26.078046824763746 29.796938824675706 24.178538223647465 90-91 19.89053885133025 26.042388678107 29.815401072954863 24.251671397607897 92-93 20.033448576829098 25.776584687310546 29.733000394457605 24.456966341402758 94-95 19.900194387109405 25.461260321051103 29.834048029740572 24.804497262098916 96-97 20.176572753555583 25.085397642721606 29.738014728676177 25.000014875046634 98-99 20.085845898639008 24.89911857420125 29.66449414108287 25.350541386076873 100-101 20.43025144101219 24.34106037082642 30.233655144517613 24.995033043643783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2595.0 1 1784.5 2 1014.5 3 1051.0 4 959.5 5 968.5 6 1344.5 7 2096.0 8 2910.5 9 3122.5 10 2665.5 11 2313.5 12 2273.0 13 2247.5 14 2359.0 15 2495.0 16 2579.5 17 2658.0 18 2718.0 19 2926.5 20 3152.5 21 3481.5 22 3929.0 23 4383.0 24 4838.0 25 5540.5 26 6753.5 27 7938.5 28 8953.0 29 10096.0 30 11453.5 31 13106.5 32 14918.0 33 16779.5 34 18966.0 35 20890.5 36 22717.5 37 24777.5 38 25628.5 39 25413.5 40 25529.0 41 26182.5 42 26711.5 43 27642.5 44 28743.5 45 29399.0 46 29460.0 47 29707.5 48 31020.0 49 30858.0 50 29281.5 51 27560.5 52 25697.5 53 24458.5 54 22964.5 55 20644.0 56 18289.5 57 16114.0 58 14367.0 59 12752.0 60 11027.0 61 9387.0 62 7546.0 63 5646.5 64 4083.5 65 3088.5 66 2333.5 67 1756.5 68 1364.5 69 985.0 70 768.0 71 649.5 72 519.0 73 457.0 74 375.5 75 288.5 76 226.5 77 157.0 78 118.5 79 78.0 80 46.0 81 33.5 82 29.0 83 28.0 84 16.5 85 12.5 86 10.5 87 6.0 88 5.5 89 4.0 90 3.5 91 3.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2677915491622627 2 0.16150437234874515 3 0.19361565988103419 4 0.16340024172334527 5 0.2392350167073489 6 0.18923146195227147 7 0.23733914733274877 8 0.24456714932341161 9 0.1449155153209944 10-11 0.20208782614877835 12-13 0.20664976183140982 14-15 0.16642178353911416 16-17 0.28254378273336966 18-19 0.22566770149536697 20-21 0.19468208640424675 22-23 0.2401829513946489 24-25 0.22637865251084202 26-27 0.33936061805341616 28-29 0.3098561509112022 30-31 0.2870464724980449 32-33 0.18360309974642747 34-35 0.3566011801786857 36-37 0.400502405384269 38-39 0.38266938407943696 40-41 0.3452259639310851 42-43 0.3785221698224992 44-45 0.3874683034338934 46-47 0.3931559115576937 48-49 0.4119961134677821 50-51 0.40399791454368794 52-53 0.4045903737232505 54-55 0.3980140768301064 56-57 0.3772780055454179 58-59 0.3513282934805792 60-61 0.3191577600303339 62-63 0.3853354503874683 64-65 0.42017205014574494 66-67 0.4256226745977202 68-69 0.43089556129582673 70-71 0.42947365926487663 72-73 0.42070526340735126 74-75 0.4160248358888073 76-77 0.4019243074152191 78-79 0.3750266606630803 80-81 0.38699433609024336 82-83 0.3955257482759438 84-85 0.3964736829632438 86-87 0.39220797687039366 88-89 0.4005616513022253 90-91 0.42070526340735126 92-93 0.4201128042277887 94-95 0.42734080621845155 96-97 0.42734080621845155 98-99 0.42621513377728276 100-101 0.4010948645638316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 843940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.47858797634893 #Duplication Level Percentage of deduplicated Percentage of total 1 84.57247082123278 56.222584418665264 2 9.649262613449565 12.82938707110203 3 2.6001112257362053 5.185551686051904 4 0.963412547842121 2.561852232769637 5 0.5206757912938914 1.7306895699343028 6 0.31863288541654466 1.2709358583193004 7 0.2227174843325696 1.036416071325169 8 0.15776146302140148 0.8390207438996606 9 0.1356114514583927 0.8113732025739417 >10 0.7775026242182771 9.571467769412545 >50 0.05950589704664365 2.714657542205326 >100 0.020548379379800526 2.268080356819578 >500 5.360446715222889E-4 0.26500928186537026 >1k 8.934077858704815E-4 1.2275239438802046 >5k 3.5736311434819256E-4 1.4654502511757386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6844 0.8109581249851885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5493 0.6508756546673935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3483 0.4127070644832571 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2594 0.3073678223570396 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.22430504538237314 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 0.14361210512595682 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1152 0.13650259497120648 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.11031589923454273 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.924591795625281E-5 0.0 20-21 0.0 0.0 0.0 5.332132616062753E-4 0.0 22-23 0.0 0.0 0.0 0.0023105908002938598 0.0 24-25 0.0 0.0 0.0 0.005983837713581535 0.0 26-27 0.0 0.0 0.0 0.010782757068038013 0.0 28-29 0.0 0.0 0.0 0.030274664075645188 0.0 30-31 0.0 0.0 0.0 0.08294428513875393 0.0 32-33 0.0 0.0 0.0 0.19722966087636562 0.0 34-35 0.0 0.0 0.0 0.38675735241841835 0.0 36-37 0.0 0.0 0.0 0.6781287769272697 0.0 38-39 0.0 0.0 0.0 1.0449795009123872 0.0 40-41 0.0 0.0 0.0 1.4404460032703748 0.0 42-43 0.0 0.0 0.0 1.8360309974642748 0.0 44-45 0.0 0.0 0.0 2.3030665687134158 0.0 46-47 0.0 0.0 0.0 2.8486029812545914 0.0 48-49 0.0 0.0 0.0 3.3967462141858427 0.0 50-51 0.0 0.0 0.0 3.949273645045856 0.0 52-53 0.0 0.0 0.0 4.487759793350238 0.0 54-55 0.0 1.1849183591250562E-4 0.0 5.088039434082992 0.0 56-57 0.0 1.1849183591250562E-4 0.0 5.760717586558286 0.0 58-59 0.0 1.1849183591250562E-4 0.0 6.466395715335214 0.0 60-61 0.0 1.1849183591250562E-4 0.0 7.217811692774368 0.0 62-63 0.0 1.1849183591250562E-4 0.0 7.952461075431902 0.0 64-65 0.0 1.1849183591250562E-4 0.0 8.738713652629333 0.0 66-67 0.0 1.1849183591250562E-4 0.0 9.587766902860393 0.0 68-69 0.0 1.1849183591250562E-4 0.0 10.510107353603336 0.0 70-71 0.0 1.1849183591250562E-4 0.0 11.484287982558001 0.0 72-73 0.0 1.1849183591250562E-4 0.0 12.459416546199968 0.0 74-75 0.0 1.1849183591250562E-4 0.0 13.471633054482545 1.1849183591250562E-4 76-77 0.0 1.1849183591250562E-4 0.0 14.544339644998459 2.3698367182501125E-4 78-79 0.0 1.1849183591250562E-4 0.0 15.702182619617508 2.3698367182501125E-4 80-81 0.0 2.3698367182501125E-4 0.0 16.88639002772709 2.3698367182501125E-4 82-83 0.0 2.3698367182501125E-4 0.0 18.067694385856818 2.3698367182501125E-4 84-85 0.0 2.3698367182501125E-4 0.0 19.23424651041543 2.3698367182501125E-4 86-87 0.0 2.3698367182501125E-4 0.0 20.465909898807972 2.3698367182501125E-4 88-89 0.0 3.5547550773751686E-4 0.0 21.694907220892482 2.3698367182501125E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3880 0.0 40.04011 1 GTATCAA 6165 0.0 34.98662 1 ATCAACG 7740 0.0 27.75963 3 TCAACGC 7825 0.0 27.458088 4 TATCAAC 7915 0.0 27.145868 2 CAACGCA 8015 0.0 26.807182 5 AACGCAG 8205 0.0 26.12551 6 GTCGGGA 295 0.0 24.117134 2 CGGGAGT 320 0.0 23.715183 4 ACGCAGA 9145 0.0 23.33639 7 CGCAGAG 9260 0.0 22.995361 8 GAGTACT 5125 0.0 22.177534 12-13 GTACATG 5015 0.0 21.978415 1 GATAGTC 110 0.0029033262 21.556696 7 GCAGAGT 9590 0.0 21.312666 9 TACATGG 5075 0.0 21.308617 2 AGTACTT 5245 0.0 21.172491 12-13 ACATGGG 5015 0.0 20.901518 3 GGTCGGG 400 0.0 20.19148 1 TCGGGAG 380 0.0 19.97068 3 >>END_MODULE