FastQCFastQC Report
Thu 26 May 2016
SRR938752_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938752_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843940
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64490.7641538497997488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54390.6444770955281182No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.44114510510225846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26350.3122259876294523No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT22210.263170367561675No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.21861743725857286No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.1451524989928194No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT12150.14396758063369436RNA PCR Primer, Index 17 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.14041282555631918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38750.042.59791
TGTACCG853.660025E-739.0891465
GTATCAA59300.032.2479251
AGTACGT650.005870848429.2025477
ATCAACG74250.025.5705813
TATCAAC76450.025.0209982
TCAACGC75900.025.0146984
CGTAGAG950.00123150624.9817832
CAACGCA76700.024.7537885
ACGATAC1551.4188699E-624.4982663
TACGATA1751.495664E-724.410772
AACGCAG78400.024.2112966
GTACATG48900.023.2498131
ATACGAT1852.5042937E-723.1420141
TACATGG50650.022.2098672
ACATGGG50550.021.4087243
CGATACT1805.198777E-621.0957284
GAGTACT51350.020.97902512-13
GTCCTAA2751.2350938E-920.7576851
ACGCAGA90400.020.734947
Produced by FastQC (version 0.11.5)