##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938752_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 843940 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.956954285849704 31.0 31.0 34.0 30.0 34.0 2 32.158699670592696 33.0 31.0 34.0 30.0 34.0 3 32.221136573690075 34.0 31.0 34.0 30.0 34.0 4 35.88714482072185 37.0 35.0 37.0 35.0 37.0 5 35.79039149702586 37.0 35.0 37.0 35.0 37.0 6 35.82318885228808 37.0 35.0 37.0 35.0 37.0 7 35.73742564637296 37.0 35.0 37.0 33.0 37.0 8 35.733577031542524 37.0 35.0 37.0 33.0 37.0 9 37.34325070502642 39.0 37.0 39.0 34.0 39.0 10-11 37.28515830509278 39.0 37.0 39.0 33.5 39.0 12-13 37.208832381448914 39.0 37.0 39.0 33.0 39.0 14-15 38.37918927885869 40.0 38.0 41.0 33.0 41.0 16-17 38.31425753015617 40.0 38.0 41.0 33.0 41.0 18-19 38.45121987345072 40.0 38.0 41.0 33.5 41.0 20-21 38.45459155864161 40.0 38.0 41.0 34.0 41.0 22-23 38.35802308220964 40.0 38.0 41.0 33.5 41.0 24-25 38.263249164632555 40.0 38.0 41.0 33.0 41.0 26-27 38.06227042206792 40.0 37.0 41.0 33.0 41.0 28-29 37.86273372514634 40.0 37.0 41.0 33.0 41.0 30-31 37.55920089105861 40.0 36.5 41.0 32.0 41.0 32-33 37.295454653174396 39.5 36.0 41.0 32.0 41.0 34-35 37.164723203071304 40.0 35.5 41.0 31.5 41.0 36-37 36.982792023129605 40.0 35.0 41.0 31.0 41.0 38-39 36.78596997416878 39.5 35.0 41.0 31.0 41.0 40-41 36.55368509609688 39.0 35.0 41.0 30.0 41.0 42-43 36.286372846410885 39.0 35.0 41.0 30.0 41.0 44-45 35.92768620992014 39.0 35.0 41.0 29.5 41.0 46-47 35.44704185131644 39.0 35.0 41.0 26.5 41.0 48-49 35.003688058392775 39.0 35.0 41.0 24.5 41.0 50-51 34.72048012891912 39.0 34.5 40.0 24.0 41.0 52-53 34.47753750266607 38.0 34.0 40.0 23.0 41.0 54-55 34.193020830864754 38.0 34.0 40.0 22.5 41.0 56-57 33.89414650330592 38.0 34.0 40.0 20.5 41.0 58-59 33.55087920942247 38.0 33.0 40.0 19.0 41.0 60-61 33.17409057515937 37.0 33.0 40.0 16.5 41.0 62-63 32.785932649240465 36.5 32.5 40.0 12.0 41.0 64-65 32.370722444723555 36.0 32.0 39.0 8.0 41.0 66-67 31.933783207337015 36.0 31.5 39.0 4.5 40.0 68-69 31.596076142853757 35.0 31.0 39.0 2.0 40.0 70-71 31.184910064696542 35.0 31.0 38.0 2.0 40.0 72-73 30.708324051472854 35.0 30.5 37.0 2.0 39.5 74-75 30.212891911747278 35.0 30.0 37.0 2.0 39.0 76-77 28.275889873687703 32.5 27.0 35.0 2.0 37.5 78-79 29.14842939071498 34.0 29.0 36.0 2.0 38.0 80-81 29.164660402398276 34.0 29.0 35.0 2.0 37.0 82-83 28.82922778870536 34.0 29.0 35.0 2.0 37.0 84-85 28.425877432044935 34.0 29.0 35.0 2.0 36.5 86-87 27.855844609806383 34.0 27.5 35.0 2.0 36.0 88-89 27.275505367680168 33.5 26.0 35.0 2.0 35.5 90-91 26.761086096167972 33.0 25.0 35.0 2.0 35.0 92-93 26.456374268312914 33.0 25.0 35.0 2.0 35.0 94-95 26.122098134938504 33.0 24.5 35.0 2.0 35.0 96-97 25.544120435102023 33.0 21.5 35.0 2.0 35.0 98-99 24.782182975093015 32.0 10.0 34.5 2.0 35.0 100-101 21.66804038201768 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07422183569958207 1101 2 0.09295146180090086 1101 3 -0.08976232708332077 1101 4 -0.0332302518500498 1101 5 0.006117312992657276 1101 6 0.0067109093039974255 1101 7 0.07105948149272479 1101 8 0.1137702972085961 1101 9 0.2786589502682446 1101 10-11 0.27979671829205444 1101 12-13 0.06071784454328366 1101 14-15 0.18007751102160086 1101 16-17 0.07349991040189963 1101 18-19 0.04675797675906068 1101 20-21 0.17383198112548115 1101 22-23 0.11667358850453269 1101 24-25 0.1495154416512392 1101 26-27 -0.1160532338879321 1101 28-29 -0.006894934235042172 1101 30-31 0.10292121638562435 1101 32-33 0.220324241064894 1101 34-35 0.2059480363397057 1101 36-37 0.465208990147147 1101 38-39 0.3142238951005396 1101 40-41 0.3113565174480044 1101 42-43 0.13066639794502777 1101 44-45 0.42949013472032505 1101 46-47 0.5308404530634263 1101 48-49 0.1433443812112145 1101 50-51 0.24701200694111236 1101 52-53 0.29944243516747804 1101 54-55 0.36990873756259646 1101 56-57 0.4493279499038181 1101 58-59 0.563410600946554 1101 60-61 0.6996898422137292 1101 62-63 0.5740371560784467 1101 64-65 0.390059752404305 1101 66-67 0.5285381279083836 1101 68-69 0.6329630058523286 1101 70-71 0.6328359270819739 1101 72-73 0.4760445594567848 1101 74-75 0.23605440895312313 1101 76-77 -0.19606827757972667 1101 78-79 -0.06681490086359787 1101 80-81 -0.006594832993496169 1101 82-83 0.2313771419943187 1101 84-85 0.04397341505421437 1101 86-87 0.14970813442646502 1101 88-89 0.04543481543673167 1101 90-91 -0.17303976156404488 1101 92-93 0.025403554846889165 1101 94-95 -0.15634969976718693 1101 96-97 -0.3480208702225127 1101 98-99 -0.3279433745310065 1101 100-101 0.060315059820784 1102 1 0.2885766041556366 1102 2 0.16909165239041357 1102 3 0.19714181510452278 1102 4 0.19675200654003078 1102 5 -0.05034775090901178 1102 6 0.040643066237564085 1102 7 0.1266109056021918 1102 8 0.126274413899516 1102 9 0.3093296256180267 1102 10-11 0.2245055752861802 1102 12-13 0.2005631632017213 1102 14-15 0.3450426121062833 1102 16-17 0.4052095854223481 1102 18-19 0.2068521029050956 1102 20-21 0.17031553029735846 1102 22-23 0.2936169322137019 1102 24-25 0.43417797923264345 1102 26-27 0.3133787158831325 1102 28-29 0.28184179939646725 1102 30-31 0.3250525467938843 1102 32-33 0.40994168096121086 1102 34-35 0.4490824582500679 1102 36-37 0.3282002043319352 1102 38-39 0.5262945640760961 1102 40-41 0.45662524440781027 1102 42-43 0.5411881287612417 1102 44-45 0.6553875733417911 1102 46-47 0.6253490529635641 1102 48-49 0.47729566083810226 1102 50-51 0.42097539760711555 1102 52-53 0.3846499695086436 1102 54-55 0.5957583694507491 1102 56-57 0.48487491205150945 1102 58-59 0.2529961696873215 1102 60-61 0.45082923602435443 1102 62-63 0.3937367173188733 1102 64-65 0.2668989836720357 1102 66-67 0.38001323122404074 1102 68-69 0.35319269064659764 1102 70-71 0.4383262648099837 1102 72-73 0.30499206766360487 1102 74-75 0.15237669007796129 1102 76-77 0.16620583560537128 1102 78-79 0.03473722441129112 1102 80-81 0.3085099699896432 1102 82-83 0.31665904236508524 1102 84-85 0.360326504962746 1102 86-87 0.4879301488959733 1102 88-89 0.31919741782282074 1102 90-91 0.2284305610782873 1102 92-93 0.4202924838698259 1102 94-95 0.618438091455495 1102 96-97 0.544626454193974 1102 98-99 0.5764537825619236 1102 100-101 1.3673511485925758 1103 1 0.12599667856837726 1103 2 0.05848570788494811 1103 3 0.1440695223576398 1103 4 0.010860628712308085 1103 5 7.152274738331243E-4 1103 6 0.09915157602446101 1103 7 0.21825323629333582 1103 8 0.09023872302742575 1103 9 0.11323558516497201 1103 10-11 0.37889995715239877 1103 12-13 0.18006134545334618 1103 14-15 0.1633004116800194 1103 16-17 0.14877793474789058 1103 18-19 0.07926986433590599 1103 20-21 0.1276452707041642 1103 22-23 0.12301039172516681 1103 24-25 0.220178904425417 1103 26-27 0.18351968007021924 1103 28-29 0.13739678734909688 1103 30-31 0.4363374863016105 1103 32-33 0.5721711117406088 1103 34-35 0.4720519297266037 1103 36-37 0.3245313609080469 1103 38-39 0.3327710553818406 1103 40-41 0.2088147727014018 1103 42-43 0.33879014829246046 1103 44-45 0.43494975282351334 1103 46-47 0.5330050231530592 1103 48-49 0.518400045854527 1103 50-51 0.5822065567374395 1103 52-53 0.34197545289349307 1103 54-55 0.39746983417848014 1103 56-57 0.4696922432393009 1103 58-59 0.5010826383173281 1103 60-61 0.6189865698798727 1103 62-63 0.6609457285829592 1103 64-65 0.8506977514060807 1103 66-67 0.7671915067895867 1103 68-69 0.8018480393108867 1103 70-71 0.8826259705078634 1103 72-73 0.9479918207563323 1103 74-75 0.9612825219142422 1103 76-77 1.202054217511325 1103 78-79 0.8388929292464162 1103 80-81 1.1559354650103444 1103 82-83 1.1925583382536509 1103 84-85 1.2175835921947993 1103 86-87 1.22876761863564 1103 88-89 1.2896494284142896 1103 90-91 1.0127747952258446 1103 92-93 1.1628384384218613 1103 94-95 1.2384747586046245 1103 96-97 1.1208420468140048 1103 98-99 0.9423469483388693 1103 100-101 1.250590259839914 1104 1 0.09479850537205436 1104 2 0.031216996064898694 1104 3 0.03713233540194949 1104 4 0.06426046703351318 1104 5 -0.13914942904207805 1104 6 -0.10498040054044822 1104 7 -0.037763187160649636 1104 8 -0.09100403684623615 1104 9 0.12211819580521421 1104 10-11 0.04468252973423148 1104 12-13 -0.18086155709767837 1104 14-15 0.0030856177926992245 1104 16-17 0.0435589322115888 1104 18-19 0.06188036567905897 1104 20-21 0.08346904082025475 1104 22-23 -0.1473119657849864 1104 24-25 -0.08193680980870965 1104 26-27 0.039674169434704254 1104 28-29 0.08025916869436145 1104 30-31 -0.16603060797213232 1104 32-33 -0.040710965526294274 1104 34-35 0.11669333338743115 1104 36-37 0.25607969925564333 1104 38-39 0.22660406507978337 1104 40-41 0.2851836948150037 1104 42-43 0.19095614407547856 1104 44-45 0.19761598056837215 1104 46-47 0.2895707933636018 1104 48-49 0.42441105223555553 1104 50-51 0.3327288181292616 1104 52-53 0.32148016574694793 1104 54-55 0.42057642828415 1104 56-57 0.23153479809758437 1104 58-59 0.29284618725016287 1104 60-61 0.2072424946297886 1104 62-63 0.5085843014759206 1104 64-65 0.3989687328407925 1104 66-67 0.31497217367293473 1104 68-69 0.3021779251634378 1104 70-71 0.2956250009907073 1104 72-73 0.42847577371946954 1104 74-75 0.3460870767002966 1104 76-77 0.11856045547917304 1104 78-79 -0.020746543714057708 1104 80-81 0.34363565363560156 1104 82-83 0.3737135915643144 1104 84-85 0.3404685030075534 1104 86-87 0.33778760068985036 1104 88-89 0.3653009356106658 1104 90-91 0.41689607654416605 1104 92-93 0.46101288483867364 1104 94-95 0.37252092317807595 1104 96-97 0.5879791654312356 1104 98-99 0.7984840752900588 1104 100-101 1.3870081300690664 1105 1 0.18292474341133413 1105 2 0.1293509561118995 1105 3 0.1205031596423396 1105 4 0.10065596812466993 1105 5 0.009464974181021546 1105 6 0.0244605932483708 1105 7 0.16311510728286294 1105 8 0.2472924211024008 1105 9 0.317627344705663 1105 10-11 0.05966523915171962 1105 12-13 0.13555475984038168 1105 14-15 0.27874249205826374 1105 16-17 0.25936084592654396 1105 18-19 0.3030057643696793 1105 20-21 0.07913665874874454 1105 22-23 0.09300228635535035 1105 24-25 0.10522719892051668 1105 26-27 0.22787091276187965 1105 28-29 0.332574092313628 1105 30-31 0.1390021266258259 1105 32-33 0.06649870377033551 1105 34-35 -0.06483831005725449 1105 36-37 -0.1236557380450094 1105 38-39 -0.008850694027145778 1105 40-41 0.0742338878651978 1105 42-43 0.04944263056196263 1105 44-45 0.10474171499845397 1105 46-47 0.09135625584472251 1105 48-49 -0.04481238237918461 1105 50-51 -0.1320474115165311 1105 52-53 0.01824900912448868 1105 54-55 -0.0486449918937879 1105 56-57 -0.09670491988126173 1105 58-59 -0.025332761885309196 1105 60-61 -0.21242646625656647 1105 62-63 -0.15496916503406766 1105 64-65 -0.19072406554765564 1105 66-67 -0.05425327537859914 1105 68-69 0.09113746855776483 1105 70-71 0.2727752444018243 1105 72-73 0.18864632936828585 1105 74-75 0.09558917507196085 1105 76-77 0.22055877678184288 1105 78-79 -0.033459654340209255 1105 80-81 -0.15292100239931017 1105 82-83 -0.19881754827116893 1105 84-85 -0.09769030175994331 1105 86-87 0.15996856426211892 1105 88-89 0.012964524665559196 1105 90-91 -0.008068838681619894 1105 92-93 0.17155778999227422 1105 94-95 0.27141768329223126 1105 96-97 0.09443677832362951 1105 98-99 0.13288595543106752 1105 100-101 1.102626058556563 1106 1 0.123533663902613 1106 2 0.13296181584041022 1106 3 0.022772067205366398 1106 4 0.10064433270126472 1106 5 -0.01805021651063754 1106 6 0.20113803345522285 1106 7 0.14471045311350395 1106 8 0.17145402420517541 1106 9 0.12166225097586647 1106 10-11 0.11986756623640105 1106 12-13 0.1429516570607987 1106 14-15 0.12641670020305895 1106 16-17 0.05509646325040052 1106 18-19 -0.05791149484169722 1106 20-21 0.004891668444926722 1106 22-23 0.02308179508192154 1106 24-25 0.08809339155141771 1106 26-27 0.09388707307215327 1106 28-29 0.10748359457607393 1106 30-31 0.1570661214545197 1106 32-33 0.08384970570956796 1106 34-35 -0.09742460611414572 1106 36-37 -0.02707267405018854 1106 38-39 0.07095473583710543 1106 40-41 0.2925519227714659 1106 42-43 -0.15174288980002615 1106 44-45 -0.09839855649476448 1106 46-47 0.04461676004640225 1106 48-49 -0.04741807080839777 1106 50-51 0.15661580761727834 1106 52-53 0.023247716299671595 1106 54-55 0.03337957177783579 1106 56-57 0.07884711631783148 1106 58-59 0.27892192460467413 1106 60-61 0.11514496231486504 1106 62-63 0.19181364039579307 1106 64-65 0.3328215065273312 1106 66-67 0.2460673451773161 1106 68-69 0.10460094625653582 1106 70-71 0.08784376411739814 1106 72-73 -0.012327167722855137 1106 74-75 0.235221366410034 1106 76-77 0.32150641737653984 1106 78-79 0.17836775354602352 1106 80-81 0.18146684504736044 1106 82-83 0.17439059652521038 1106 84-85 0.18078545777463617 1106 86-87 0.22933055521234635 1106 88-89 0.4477757722415099 1106 90-91 0.49455624211993054 1106 92-93 0.34414279322433927 1106 94-95 0.5148966748888668 1106 96-97 0.522602259900875 1106 98-99 0.7015692133496181 1106 100-101 1.3107669764621868 1107 1 -0.15059906611247342 1107 2 -0.11088978462883858 1107 3 -0.0911304382412581 1107 4 -0.10187577790707536 1107 5 -0.3586474596814071 1107 6 -0.14156453902675992 1107 7 -0.03351981335205778 1107 8 -0.0917697482097708 1107 9 -0.21522186164354196 1107 10-11 -0.17701267090118478 1107 12-13 -0.055729631694013904 1107 14-15 -0.15467880423803138 1107 16-17 -0.11354722285651775 1107 18-19 -0.13783021446629817 1107 20-21 -0.2475147962777129 1107 22-23 -0.07277615279807748 1107 24-25 -0.19979991748159875 1107 26-27 -0.20488900787304232 1107 28-29 -0.30582868546414943 1107 30-31 -0.3384482701995779 1107 32-33 -0.2778584390868133 1107 34-35 -0.28579135238529574 1107 36-37 -0.3060340449233223 1107 38-39 -0.5179849532864083 1107 40-41 -0.7627548687485657 1107 42-43 -0.7837842212898849 1107 44-45 -0.7468740257422866 1107 46-47 -0.7633897759013095 1107 48-49 -0.5553785199453145 1107 50-51 -0.69962214696627 1107 52-53 -0.6672714935210138 1107 54-55 -0.8027739601477606 1107 56-57 -0.7974595495108971 1107 58-59 -0.5817335555361041 1107 60-61 -0.3582346673147683 1107 62-63 -0.5312700909599926 1107 64-65 -0.6662200972936816 1107 66-67 -0.6191732489235733 1107 68-69 -0.6454776108073155 1107 70-71 -0.769499226497647 1107 72-73 -0.667157903435946 1107 74-75 -0.4203294757216902 1107 76-77 -0.5319637099906451 1107 78-79 -0.40554166492222166 1107 80-81 -0.31033965319296186 1107 82-83 -0.3447249556785792 1107 84-85 -0.5744647726594145 1107 86-87 -0.7667258801823245 1107 88-89 -0.8726584912074564 1107 90-91 -0.6032368280996074 1107 92-93 -0.6811578184733342 1107 94-95 -0.5519606235860799 1107 96-97 -0.636656475644628 1107 98-99 -0.2325816371615268 1107 100-101 0.6358099209904218 1108 1 0.030919085702706184 1108 2 0.020321253141602824 1108 3 -0.046243657896553714 1108 4 -0.02177401773106169 1108 5 -0.055486935295640194 1108 6 -0.01490291453097825 1108 7 0.07470350898017841 1108 8 -0.00975425499377991 1108 9 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-0.6523019902711127 2316 52-53 -0.5773053254298475 2316 54-55 -0.7938263105751133 2316 56-57 -0.8306237233000644 2316 58-59 -0.44952201707455686 2316 60-61 -0.339449054779152 2316 62-63 -0.7438324128973193 2316 64-65 -0.6509957383193239 2316 66-67 -0.650111639236961 2316 68-69 -0.8567489783286852 2316 70-71 -0.7447039985774175 2316 72-73 -0.6165881028661424 2316 74-75 -0.8165634500806682 2316 76-77 -0.5457635675405044 2316 78-79 -0.3982410951216515 2316 80-81 -0.7279411489194558 2316 82-83 -0.8240696850233071 2316 84-85 -0.9545840746537202 2316 86-87 -1.0367579314643756 2316 88-89 -1.0972845595835246 2316 90-91 -1.3073856884984671 2316 92-93 -1.2554989865645005 2316 94-95 -1.3167398258652803 2316 96-97 -1.6958178004309516 2316 98-99 -1.9264473779022673 2316 100-101 -1.8331181416876383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 16.0 5 43.0 6 115.0 7 314.0 8 598.0 9 997.0 10 1861.0 11 2757.0 12 3805.0 13 5127.0 14 5929.0 15 7556.0 16 9549.0 17 12677.0 18 11953.0 19 7400.0 20 6684.0 21 6940.0 22 7220.0 23 7911.0 24 8900.0 25 9890.0 26 11258.0 27 13253.0 28 15837.0 29 18431.0 30 22462.0 31 26864.0 32 32532.0 33 40344.0 34 52198.0 35 72774.0 36 102387.0 37 159842.0 38 143372.0 39 14141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.94553749271026 21.160846951731543 15.46968478639138 21.423930769166812 2 16.09984122093988 22.681470246700002 39.01592530274664 22.202763229613478 3 18.356399744057633 27.307865488067872 32.27836102092566 22.057373746948837 4 12.861222361779273 19.01675474559803 37.28784036779866 30.834182524824037 5 12.44596889752254 38.63382349978133 34.40212296157406 14.51808464112207 6 28.430164354018792 37.52141816070434 18.593807396523328 15.45461008875354 7 25.159726994810054 35.38877171362893 22.091499395691635 17.360001895869377 8 25.05047752209873 34.48313861174965 21.53920894850345 18.927174917648173 9 26.079816100670666 16.776548095836198 22.193520866412303 34.95011493708083 10-11 23.58651088939972 27.86448088726687 28.068583074626158 20.480425148707255 12-13 24.992120292911817 26.131478541128516 28.49195440434154 20.384446761618126 14-15 22.27237718321208 27.102756120103326 27.992452070052376 22.632414626632226 16-17 20.173559824935285 30.099482590990934 28.723754068086123 21.00320351598766 18-19 19.825283787947008 27.936109202075976 32.319596179823215 19.919010830153802 20-21 21.30643173685333 26.970104509799274 32.150982297319715 19.57248145602768 22-23 20.93809986491931 27.48815081640875 31.595848046069623 19.977901272602317 24-25 20.43805480395125 27.69352634176427 32.15411604322842 19.714302811056058 26-27 20.332967977012174 27.84755754362058 32.0979352430607 19.721539236306544 28-29 19.84299831741593 27.9420337938716 32.26704505059602 19.94792283811645 30-31 20.44410740100007 27.715655141360763 32.0148944237742 19.825343033864968 32-33 19.877360949830557 28.06413963077944 31.82033082920587 20.238168590184134 34-35 20.324488879356213 27.934388853404684 31.867467095479103 19.87365517176 36-37 20.360241954789913 27.73307229015613 31.591004439472194 20.315681315581767 38-39 20.107153141772304 27.64964643729432 31.830835028956944 20.41236539197643 40-41 20.108286051505196 27.523415762505394 31.922284790656448 20.44601339533296 42-43 20.19134373141014 27.629539481012564 31.47738880448977 20.701727983087522 44-45 20.059840504319183 27.960801507269732 31.15492173336019 20.824436255050895 46-47 20.396641686479594 27.83915358460451 30.559148181347982 21.205056547567914 48-49 20.41353176317104 27.986830591088907 30.212737972723897 21.38689967301616 50-51 20.16098310364665 28.367673275157756 30.03349173726316 21.43785188393244 52-53 20.143019645946396 28.563345735478823 29.93559968718155 21.358034931393227 54-55 19.91794440363059 28.37162594497239 29.839739791928338 21.870689859468683 56-57 20.180619566704998 28.724175093032173 29.238081188157526 21.857124152105303 58-59 19.971668450965744 28.390736021887932 29.618673464974517 22.018922062171814 60-61 20.166556170345633 28.663634386929086 29.348059856419955 21.821749586305327 62-63 19.96806616642919 28.57213106498446 29.381200510704353 22.078602257882 64-65 19.714773401206624 28.486933976992045 29.33730524791584 22.46098737388549 66-67 19.848641679647205 28.644677662946005 28.860641445019887 22.6460392123869 68-69 19.914164208939766 28.48559785860793 28.830825049737125 22.76941288271518 70-71 19.851375514107062 28.436000706212177 28.70681411808199 23.005809661598764 72-73 19.932755883119654 28.39520582031898 28.427731829276965 23.244306467284403 74-75 19.733261605892867 28.583878744287922 28.16204555916892 23.520814090650294 76-77 19.633985052798938 28.501171695998096 28.513857906146946 23.350985345056024 78-79 19.584264290062187 28.463248023484432 28.265626130941857 23.68686155551152 80-81 19.477148578673585 28.54448828694323 28.124429751282687 23.853933383100497 82-83 19.604825044731196 28.426765253042312 27.892716220539622 24.07569348168687 84-85 19.428869350901724 28.095480721378298 27.896651420717113 24.578998507002865 86-87 19.46815267925294 28.145692811259913 27.835869625169696 24.550284884317456 88-89 19.551016598164136 27.930601278410023 27.806517857726792 24.71186426569905 90-91 19.37874397514405 27.844231601886083 27.78386409162063 24.993160331349234 92-93 19.440456857934553 27.751666738749332 27.674172940326812 25.133703462989303 94-95 19.303632854111147 27.555018917288848 27.77452582361006 25.366822404989946 96-97 19.370512121714814 27.388499182406335 27.66381496314904 25.577173732729815 98-99 19.226959262506814 27.471561959380995 27.42576486480082 25.875713913311372 100-101 19.315321143912072 27.20882160040809 27.894720939036898 25.58113631664294 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1961.0 1 1321.0 2 786.5 3 882.5 4 789.5 5 790.0 6 1147.0 7 1931.5 8 2724.5 9 2924.0 10 2475.0 11 2031.0 12 1985.0 13 2018.0 14 2107.5 15 2216.5 16 2272.0 17 2417.5 18 2603.0 19 2781.0 20 3025.0 21 3359.5 22 3760.5 23 4202.0 24 4740.5 25 5456.0 26 6650.0 27 7972.5 28 9015.5 29 10246.5 30 11729.0 31 13338.5 32 15016.5 33 16855.0 34 19044.5 35 20705.5 36 22384.5 37 24633.0 38 25429.0 39 25100.5 40 25476.5 41 26316.5 42 27068.5 43 28213.0 44 29593.5 45 30373.5 46 30565.0 47 30766.5 48 31673.0 49 31626.0 50 30188.0 51 28424.0 52 26340.0 53 24712.0 54 23143.0 55 20932.5 56 18366.0 57 16009.5 58 14471.5 59 12778.5 60 10761.0 61 9051.5 62 7284.5 63 5371.5 64 3750.5 65 2756.5 66 1995.0 67 1489.0 68 1132.5 69 781.5 70 566.0 71 461.5 72 387.0 73 345.0 74 280.5 75 218.5 76 159.0 77 86.5 78 50.0 79 38.5 80 30.5 81 22.0 82 17.0 83 10.5 84 4.0 85 1.5 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23733914733274877 2 0.0 3 0.0 4 0.0 5 0.0252387610493637 6 0.003554755077375169 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027845581439438823 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.332132616062753E-4 26-27 0.0017773775386875845 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006991018318837833 36-37 0.017299808043225822 38-39 0.011967675427163068 40-41 0.00758347749840036 42-43 0.007820461170225371 44-45 0.003554755077375169 46-47 5.332132616062753E-4 48-49 0.0017181316207313317 50-51 5.924591795625281E-5 52-53 0.0 54-55 0.0 56-57 0.0013626561129938147 58-59 5.332132616062753E-4 60-61 0.0024290826362063653 62-63 8.886887693437922E-4 64-65 0.0017181316207313317 66-67 0.005746854041756523 68-69 3.554755077375169E-4 70-71 1.1849183591250562E-4 72-73 0.0 74-75 5.332132616062753E-4 76-77 0.05977913121785909 78-79 0.13851695618171908 80-81 0.0011849183591250564 82-83 0.0011849183591250564 84-85 0.0 86-87 0.1040950778491362 88-89 0.3523354740858355 90-91 0.3858094177311183 92-93 1.7773775386875846E-4 94-95 3.554755077375169E-4 96-97 0.0 98-99 0.0 100-101 0.0013626561129938147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 843940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.10978946141775 #Duplication Level Percentage of deduplicated Percentage of total 1 83.21135730733083 53.346625977717856 2 10.546366134194436 13.522506248924632 3 2.7206637351816143 5.2326353777342325 4 1.114241420561202 2.857351315254788 5 0.5714863137673271 1.8318933627852534 6 0.34991156407453144 1.3459654021760161 7 0.23992545963758732 1.0767099492659724 8 0.16923795808205092 0.8679847889216422 9 0.13608119372348085 0.7851723011343684 >10 0.8440648070076504 9.994785935055814 >50 0.06801756268803703 2.95532664525151 >100 0.026059114025470797 2.735235730068726 >500 9.240820449325445E-4 0.37465086860621827 >1k 0.0012937148629055624 1.6646015348035295 >5k 3.696328179730178E-4 1.4085545622994065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6449 0.7641538497997488 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5439 0.6444770955281182 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.44114510510225846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2635 0.3122259876294523 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 2221 0.263170367561675 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1845 0.21861743725857286 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.1451524989928194 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 1215 0.14396758063369436 RNA PCR Primer, Index 17 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.14041282555631918 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 5.924591795625281E-5 0.0 20-21 0.0 0.0 0.0 5.332132616062753E-4 0.0 22-23 0.0 0.0 0.0 0.0023105908002938598 0.0 24-25 0.0 0.0 0.0 0.005983837713581535 0.0 26-27 0.0 0.0 0.0 0.010664265232125505 0.0 28-29 0.0 0.0 0.0 0.030630139583382704 0.0 30-31 0.0 0.0 0.0 0.08513638410313529 0.0 32-33 0.0 0.0 0.0 0.20262103941038462 0.0 34-35 0.0 0.0 0.0 0.3971253880607626 0.0 36-37 0.0 0.0 0.0 0.6971467165912268 0.0 38-39 0.0 0.0 0.0 1.0728843282697822 0.0 40-41 0.0 0.0 0.0 1.477119226485295 0.0 42-43 0.0 0.0 0.0 1.8784510747209517 0.0 44-45 0.0 0.0 0.0 2.3527146479607555 0.0 46-47 0.0 0.0 0.0 2.9075526696210634 0.0 48-49 0.0 0.0 0.0 3.464997511671446 0.0 50-51 0.0 0.0 0.0 4.025819371045335 0.0 52-53 0.0 0.0 0.0 4.5749697845818424 0.0 54-55 0.0 0.0 0.0 5.185025001777378 0.0 56-57 0.0 0.0 0.0 5.867537976633409 0.0 58-59 0.0 0.0 0.0 6.58441358390407 0.0 60-61 0.0 0.0 0.0 7.348982155129511 0.0 62-63 0.0 0.0 0.0 8.097139607081072 0.0 64-65 0.0 0.0 0.0 8.898085171931655 0.0 66-67 0.0 0.0 0.0 9.76443823020594 0.0 68-69 0.0 0.0 0.0 10.703071308386853 0.0 70-71 0.0 0.0 0.0 11.694196269877004 0.0 72-73 0.0 0.0 0.0 12.68769106808541 0.0 74-75 0.0 0.0 0.0 13.718155319098514 0.0 76-77 0.0 0.0 0.0 14.812368177832546 0.0 78-79 0.0 0.0 0.0 15.991658174751759 0.0 80-81 0.0 0.0 0.0 17.194113325591864 0.0 82-83 0.0 0.0 0.0 18.39473185297533 0.0 84-85 0.0 0.0 0.0 19.579531720264477 0.0 86-87 0.0 0.0 0.0 20.831753442187832 0.0 88-89 0.0 0.0 0.0 22.076865653956443 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3875 0.0 42.5979 1 TGTACCG 85 3.660025E-7 39.089146 5 GTATCAA 5930 0.0 32.247925 1 AGTACGT 65 0.0058708484 29.202547 7 ATCAACG 7425 0.0 25.570581 3 TATCAAC 7645 0.0 25.020998 2 TCAACGC 7590 0.0 25.014698 4 CGTAGAG 95 0.001231506 24.981783 2 CAACGCA 7670 0.0 24.753788 5 ACGATAC 155 1.4188699E-6 24.498266 3 TACGATA 175 1.495664E-7 24.41077 2 AACGCAG 7840 0.0 24.211296 6 GTACATG 4890 0.0 23.249813 1 ATACGAT 185 2.5042937E-7 23.142014 1 TACATGG 5065 0.0 22.209867 2 ACATGGG 5055 0.0 21.408724 3 CGATACT 180 5.198777E-6 21.095728 4 GAGTACT 5135 0.0 20.979025 12-13 GTCCTAA 275 1.2350938E-9 20.757685 1 ACGCAGA 9040 0.0 20.73494 7 >>END_MODULE