FastQCFastQC Report
Thu 26 May 2016
SRR938751_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938751_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4274154
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100060.2341048076414654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77610.18157979333454058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50620.11843279395173875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102700.035.9152221
GTATCAA161400.034.3384251
ATCAACG191750.028.6396793
TCAACGC194600.028.2689364
TATCAAC195550.028.083142
CAACGCA196450.027.9786035
AACGCAG202300.027.1725836
ACGCAGA227650.023.9618387
GTACATG157950.023.7124731
CGCAGAG228100.023.7069748
TACATGG157050.022.98992
ACATGGG156750.022.0363673
GCAGAGT241700.021.530869
GAGTACT115550.021.1457312-13
AGAGTAC198400.019.83351710-11
GTACTTT126900.019.23612614-15
AGTACTT125150.018.97505212-13
CATGGGA82450.018.6772424
CAGAGTA235450.018.04995510-11
CATGGGG79750.017.8242234
Produced by FastQC (version 0.11.5)