FastQCFastQC Report
Thu 26 May 2016
SRR938751_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938751_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4274154
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99030.23169497402292946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84200.19699804920459116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54900.12844647151225716No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110500.035.0504761
GTATCAA164500.030.668721
ATCAACG198950.025.1165543
TCAACGC201450.024.8048554
TATCAAC203150.024.7608012
CAACGCA202200.024.7597895
AACGCAG209050.024.0165776
GTACATG151700.022.8618551
TACATGG152450.022.06962
ACGCAGA234300.021.2460827
CGCAGAG236650.021.015058
GAGTACT118000.020.89269312-13
ACATGGG154350.020.7833563
AGTACTT125300.019.35355212-13
AGAGTAC204700.019.14919510-11
GCAGAGT258550.018.9046369
GTACTTT129600.018.69311314-15
CATGGGG79450.017.3212724
CAGAGTA248500.016.85297810-11
CATGGGA82600.016.7181644
Produced by FastQC (version 0.11.5)