FastQCFastQC Report
Thu 26 May 2016
SRR938750_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938750_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265673
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99670.23365597878693467No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76880.18022947375478618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52170.12230192047069713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105850.033.318231
GTATCAA160600.031.5190351
ATCAACG191250.026.2213763
CAACGCA193500.026.0145515
TCAACGC193500.025.9655134
TATCAAC196300.025.5951442
AACGCAG200400.025.212656
GTACATG148950.023.2943821
TACATGG149550.022.7465062
ACGCAGA224400.022.3263577
CGCAGAG226550.022.0095088
ACATGGG151600.021.2496833
GAGTACT112950.021.05575612-13
GCAGAGT246400.019.715169
AGAGTAC191250.019.65655110-11
AGTACTT122350.019.28287512-13
GTACTTT125050.019.01859914-15
TAGCGCG1350.00946846717.5718784
CATGGGA79700.017.4417954
CAGAGTA236950.016.88707710-11
Produced by FastQC (version 0.11.5)