##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938750_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4265673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.413638363747058 33.0 31.0 34.0 30.0 34.0 2 31.49676076905098 33.0 31.0 34.0 28.0 34.0 3 31.515710416621246 33.0 31.0 34.0 28.0 34.0 4 35.07308412998371 37.0 35.0 37.0 32.0 37.0 5 35.10299242346987 37.0 35.0 37.0 32.0 37.0 6 35.14638815492889 37.0 35.0 37.0 32.0 37.0 7 35.05319910832359 37.0 35.0 37.0 32.0 37.0 8 35.01875366442763 37.0 35.0 37.0 32.0 37.0 9 36.57038830683927 39.0 37.0 39.0 32.0 39.0 10-11 36.44657349496785 39.0 37.0 39.0 32.0 39.0 12-13 36.33823830377996 39.0 37.0 39.0 32.0 39.0 14-15 37.486877568908824 40.0 37.0 41.0 32.0 41.0 16-17 37.37016984189834 40.0 37.0 41.0 32.0 41.0 18-19 37.302054447211496 40.0 37.0 41.0 31.0 41.0 20-21 37.18483953176908 40.0 37.0 41.0 31.0 41.0 22-23 37.18777728625706 40.0 37.0 41.0 31.0 41.0 24-25 37.130296321354216 40.0 37.0 41.0 31.0 41.0 26-27 37.00563697686157 40.0 36.5 41.0 30.0 41.0 28-29 36.833523924595255 39.5 36.0 41.0 30.0 41.0 30-31 36.62569657824217 39.0 36.0 41.0 30.0 41.0 32-33 36.4627732833717 39.0 36.0 41.0 30.0 41.0 34-35 36.185891651797974 39.0 35.0 41.0 29.5 41.0 36-37 35.986830682989535 39.0 35.0 41.0 28.5 41.0 38-39 35.83211664372773 39.0 35.0 40.5 27.0 41.0 40-41 35.738380672873895 39.0 35.0 40.5 27.0 41.0 42-43 35.76572946402595 39.0 35.0 41.0 27.0 41.0 44-45 35.614231564397926 39.0 35.0 41.0 26.5 41.0 46-47 35.35610406611102 39.0 35.0 41.0 25.5 41.0 48-49 35.11148135358711 39.0 35.0 41.0 24.5 41.0 50-51 33.997615147715265 38.0 33.0 39.5 23.5 40.5 52-53 34.08254008218633 38.0 33.5 39.5 23.5 40.5 54-55 34.59651865016376 38.0 34.0 40.0 24.0 41.0 56-57 34.510327678657035 38.0 34.0 40.0 23.0 41.0 58-59 34.31554094746597 38.0 34.0 40.0 23.0 41.0 60-61 34.018535644902926 38.0 33.5 40.0 22.5 41.0 62-63 33.62456604151326 37.0 33.0 40.0 21.5 41.0 64-65 33.23382511974078 37.0 32.5 40.0 20.0 41.0 66-67 32.85021871109201 36.0 32.0 39.0 19.0 41.0 68-69 32.42092736597485 36.0 31.5 39.0 18.0 40.5 70-71 32.002910912299185 35.0 31.0 38.5 14.5 40.0 72-73 31.56886849976545 35.0 31.0 38.0 12.0 40.0 74-75 31.08841664609547 35.0 31.0 37.0 8.0 39.0 76-77 30.615650215100878 35.0 30.0 36.5 7.0 39.0 78-79 30.163729967111873 34.0 30.0 36.0 2.0 39.0 80-81 29.680303787936865 34.0 29.0 36.0 2.0 37.5 82-83 29.25228961526118 34.0 29.0 35.0 2.0 37.0 84-85 28.886683062672645 34.0 29.0 35.0 2.0 36.5 86-87 28.441937884127547 34.0 28.5 35.0 2.0 36.0 88-89 27.97563749026238 34.0 27.5 35.0 2.0 36.0 90-91 27.501649094996264 34.0 26.0 35.0 2.0 35.0 92-93 27.012373311315706 33.0 25.5 35.0 2.0 35.0 94-95 26.47168676548812 33.0 25.0 35.0 2.0 35.0 96-97 25.84377084225631 33.0 23.5 35.0 2.0 35.0 98-99 25.047286442256592 32.0 18.0 35.0 2.0 35.0 100-101 22.626045292266895 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14334412850441325 1101 2 0.23311396850568045 1101 3 0.2525582408362972 1101 4 0.09337548057477107 1101 5 0.07443450644151284 1101 6 0.2541924163455036 1101 7 0.2660277547714429 1101 8 0.3278935497697688 1101 9 0.40499751378101223 1101 10-11 0.351394609309736 1101 12-13 0.3272404072972037 1101 14-15 0.4016169458525525 1101 16-17 0.5250213291634722 1101 18-19 0.5190061343547967 1101 20-21 0.5948557113022588 1101 22-23 0.49422242979471065 1101 24-25 0.49055761420794397 1101 26-27 0.4032895411451136 1101 28-29 0.5528914073127495 1101 30-31 0.4964088785303318 1101 32-33 0.41784598199760126 1101 34-35 0.59920791448166 1101 36-37 0.5248602993539393 1101 38-39 0.49716252883933976 1101 40-41 0.5617807891481377 1101 42-43 0.5798419687876901 1101 44-45 0.47310356992483804 1101 46-47 0.5965281037327514 1101 48-49 0.5152543147156052 1101 50-51 0.5339190856916076 1101 52-53 0.6392508649460495 1101 54-55 0.6311359637206593 1101 56-57 0.6144947878569553 1101 58-59 0.6445786259739137 1101 60-61 0.5026767502901279 1101 62-63 0.41329544162586984 1101 64-65 0.4198796141987273 1101 66-67 0.43873616257494774 1101 68-69 0.4466303834835088 1101 70-71 0.3954129883533568 1101 72-73 0.4472446946005988 1101 74-75 0.41340864594917903 1101 76-77 0.3989897351813738 1101 78-79 0.2458312237664977 1101 80-81 0.18186664229841298 1101 82-83 0.15497505128168498 1101 84-85 0.040606442671951015 1101 86-87 -0.07547558128888454 1101 88-89 -0.11195381012105798 1101 90-91 -0.15083143261541565 1101 92-93 -0.24494002507892176 1101 94-95 -0.1772471241535527 1101 96-97 -0.2595484657206981 1101 98-99 -0.26425716937945865 1101 100-101 0.07254327308866948 1102 1 0.12331762307723437 1102 2 0.20607546177195957 1102 3 0.17320788978411983 1102 4 0.32100121445457575 1102 5 0.2583325093396027 1102 6 0.3096348039735446 1102 7 0.2929912228805307 1102 8 0.32205051871194 1102 9 0.3677895119392218 1102 10-11 0.49529875738971185 1102 12-13 0.4307474707040839 1102 14-15 0.4628777626732301 1102 16-17 0.6248194999237953 1102 18-19 0.6225342473289146 1102 20-21 0.6701245600741217 1102 22-23 0.6058606445119281 1102 24-25 0.5039402068558374 1102 26-27 0.5629735839114929 1102 28-29 0.6041509494162582 1102 30-31 0.6757332831284941 1102 32-33 0.7787508526913172 1102 34-35 0.7888978658057866 1102 36-37 0.7532570873954896 1102 38-39 0.6390901020050777 1102 40-41 0.7745265633961438 1102 42-43 0.8032492754477119 1102 44-45 0.7981523376913984 1102 46-47 0.8701264447567638 1102 48-49 0.7618708096262168 1102 50-51 0.8320512991683913 1102 52-53 0.7324342752566082 1102 54-55 0.6202621448083576 1102 56-57 0.62477528493676 1102 58-59 0.568755266426578 1102 60-61 0.5964679558455259 1102 62-63 0.47064837839866414 1102 64-65 0.30893342235314236 1102 66-67 0.37235052475633523 1102 68-69 0.5293934947070085 1102 70-71 0.4244878425520753 1102 72-73 0.24862596896429423 1102 74-75 0.16001213533916925 1102 76-77 0.28285023051027736 1102 78-79 0.20276078533835928 1102 80-81 0.21744816140076395 1102 82-83 0.15011183794576155 1102 84-85 0.10581230655881413 1102 86-87 0.08494804404079659 1102 88-89 -0.014999268555090595 1102 90-91 -0.06571984143194953 1102 92-93 -0.15325958909864212 1102 94-95 -0.2232595405211555 1102 96-97 -0.3683097007437226 1102 98-99 -0.5816830286641874 1102 100-101 -0.1868413549965986 1103 1 0.32862270868018584 1103 2 0.4111673764251016 1103 3 0.4549014187713709 1103 4 0.4283518608746206 1103 5 0.4292418724463687 1103 6 0.4051762975982385 1103 7 0.5139880493663398 1103 8 0.40820800273548485 1103 9 0.5822414436736523 1103 10-11 0.45844874970578076 1103 12-13 0.5937772184943739 1103 14-15 0.6434551907061703 1103 16-17 0.654345747769284 1103 18-19 0.7715325423640635 1103 20-21 0.7278022901308816 1103 22-23 0.7102023585225865 1103 24-25 0.7144236779946453 1103 26-27 0.743693557431115 1103 28-29 0.8018080021785678 1103 30-31 0.8647734252608785 1103 32-33 0.7728314320007783 1103 34-35 0.8718358931269918 1103 36-37 0.9659326808521911 1103 38-39 0.9270333947350551 1103 40-41 0.9838659020322567 1103 42-43 1.0901232037314799 1103 44-45 1.1319695873005386 1103 46-47 1.0157071578086914 1103 48-49 1.0653281432448551 1103 50-51 1.0117879821260019 1103 52-53 1.0430319726536226 1103 54-55 1.1162152626190434 1103 56-57 1.1132822637969397 1103 58-59 0.9873729842868642 1103 60-61 1.04978176298836 1103 62-63 0.9043460566534449 1103 64-65 0.7849394928634226 1103 66-67 0.8082527531090804 1103 68-69 0.7956293681040592 1103 70-71 0.8224902034850743 1103 72-73 0.8496074644025242 1103 74-75 0.8209864951603443 1103 76-77 0.8348403237284074 1103 78-79 0.80977254460743 1103 80-81 0.8779147946236598 1103 82-83 0.8139076202909159 1103 84-85 0.8654773076919859 1103 86-87 0.7501243743900616 1103 88-89 0.7307288115019084 1103 90-91 0.5349691984225053 1103 92-93 0.4434029075491672 1103 94-95 0.5903484126136895 1103 96-97 0.7652138792716485 1103 98-99 0.727525893078024 1103 100-101 0.904098724289188 1104 1 0.13340896415027004 1104 2 0.15627385829747453 1104 3 0.17438193825189074 1104 4 0.2921836043523811 1104 5 0.17695332474350067 1104 6 0.2696092315404215 1104 7 0.38147059182876575 1104 8 0.33326171877874344 1104 9 0.372518310378517 1104 10-11 0.3246725196302762 1104 12-13 0.4306386089358085 1104 14-15 0.45543531555838257 1104 16-17 0.6318053410776301 1104 18-19 0.5420150224201663 1104 20-21 0.6395435483725436 1104 22-23 0.4335339137929992 1104 24-25 0.4260127043061246 1104 26-27 0.4522399782864639 1104 28-29 0.3107184694156686 1104 30-31 0.5082243601840588 1104 32-33 0.46424446114509976 1104 34-35 0.5309539814486186 1104 36-37 0.7021978507456836 1104 38-39 0.5601102784957632 1104 40-41 0.6577155287378815 1104 42-43 0.6324085802887822 1104 44-45 0.6802638470187716 1104 46-47 0.7566228140904059 1104 48-49 0.6850595023165198 1104 50-51 0.750394152107873 1104 52-53 0.7777953523888499 1104 54-55 0.6513447783599844 1104 56-57 0.632733952114279 1104 58-59 0.6061005331833513 1104 60-61 0.6683667374059894 1104 62-63 0.6772758817152109 1104 64-65 0.8388246827099906 1104 66-67 0.7120254844763423 1104 68-69 0.64558112320465 1104 70-71 0.7726355594228664 1104 72-73 0.5668164779108125 1104 74-75 0.4648491707205231 1104 76-77 0.5413105337384394 1104 78-79 0.32858192769677785 1104 80-81 0.3885723004541326 1104 82-83 0.3361680456084599 1104 84-85 0.4814658960860463 1104 86-87 0.4293594421207061 1104 88-89 0.6901694643456153 1104 90-91 0.5864201457704752 1104 92-93 0.3971180189472996 1104 94-95 0.471813250313442 1104 96-97 0.46108269267867286 1104 98-99 0.5306211344677152 1104 100-101 0.8347859253705963 1105 1 0.030523158416329466 1105 2 0.0979656504779669 1105 3 0.15455459796212168 1105 4 0.19587551937377867 1105 5 0.23382483998600634 1105 6 0.174192698520109 1105 7 0.14268234906784727 1105 8 0.17767389094709074 1105 9 0.23654435264943885 1105 10-11 0.26917409267625203 1105 12-13 0.343901604479413 1105 14-15 0.3483019444628397 1105 16-17 0.400575498928184 1105 18-19 0.3786575076280698 1105 20-21 0.36447233477269947 1105 22-23 0.3785239841535386 1105 24-25 0.47740142068695945 1105 26-27 0.4816166972060145 1105 28-29 0.49139464032830205 1105 30-31 0.41634318828186423 1105 32-33 0.558347688448535 1105 34-35 0.564700697160653 1105 36-37 0.6466389587706018 1105 38-39 0.5999539975722783 1105 40-41 0.6826732509946041 1105 42-43 0.5841600867959329 1105 44-45 0.59418226701181 1105 46-47 0.6872849947893656 1105 48-49 0.7285264819999995 1105 50-51 0.831439391681748 1105 52-53 0.8899516879082938 1105 54-55 0.7449002717368174 1105 56-57 0.5925518746016678 1105 58-59 0.6699540522339333 1105 60-61 0.7263258040881198 1105 62-63 0.7452217771230139 1105 64-65 0.7081865242315857 1105 66-67 0.6700500663054427 1105 68-69 0.6837114846981223 1105 70-71 0.4887271714367074 1105 72-73 0.5577416746268682 1105 74-75 0.4576390923070832 1105 76-77 0.4271494347218514 1105 78-79 0.5762667179459413 1105 80-81 0.49002086765151986 1105 82-83 0.4235941875170006 1105 84-85 0.4830644656720047 1105 86-87 0.4712406822678403 1105 88-89 0.63233569538205 1105 90-91 0.5781798416710018 1105 92-93 0.5495198997779802 1105 94-95 0.6251483125187107 1105 96-97 0.6662185342412101 1105 98-99 0.737188072521775 1105 100-101 1.0550183676524334 1106 1 0.07340494610461334 1106 2 0.04808684789579942 1106 3 0.15631565234801315 1106 4 0.21398424896324286 1106 5 0.21540989968813307 1106 6 0.25462596082971345 1106 7 0.21195996393642247 1106 8 0.13295330953702234 1106 9 0.09472781537252928 1106 10-11 0.24075226975738673 1106 12-13 0.22973635301323014 1106 14-15 0.30205326297261337 1106 16-17 0.389432087257795 1106 18-19 0.28160553724706716 1106 20-21 0.2986238737372915 1106 22-23 0.19664402655681812 1106 24-25 0.12614240208014849 1106 26-27 0.07779526034681084 1106 28-29 0.09601282626982766 1106 30-31 0.07757370070750369 1106 32-33 0.13747913332428396 1106 34-35 0.22469568682353014 1106 36-37 0.4091472653821384 1106 38-39 0.3393042886992319 1106 40-41 0.5018137144771586 1106 42-43 0.5125027727585518 1106 44-45 0.5677749424263467 1106 46-47 0.40740463636566915 1106 48-49 0.47380065292105655 1106 50-51 0.3838708819142269 1106 52-53 0.18645017425907895 1106 54-55 0.11986920764669406 1106 56-57 0.3901590958159602 1106 58-59 0.4526430210538166 1106 60-61 0.4216394248214286 1106 62-63 0.4354382236864467 1106 64-65 0.49091309695003105 1106 66-67 0.42488662383465936 1106 68-69 0.40518716082993933 1106 70-71 0.35763362580572533 1106 72-73 0.42199051961230793 1106 74-75 0.3402905363389799 1106 76-77 0.3261195203722451 1106 78-79 0.3594334355949833 1106 80-81 0.29228274478750293 1106 82-83 0.1918112722391605 1106 84-85 0.34036481692300313 1106 86-87 0.40201206322223015 1106 88-89 0.4888578094278735 1106 90-91 0.5929267405360292 1106 92-93 0.6558152565139821 1106 94-95 0.661912214885696 1106 96-97 0.5813271794460562 1106 98-99 0.5464169552693399 1106 100-101 1.0236451397295632 1107 1 0.0510223533060703 1107 2 0.04025348999707745 1107 3 0.1662368937598977 1107 4 0.18673132372800438 1107 5 0.12617117268887057 1107 6 -0.008551520469879392 1107 7 0.009265259508282497 1107 8 0.06103736838675644 1107 9 0.11956143372019312 1107 10-11 0.1458499820777135 1107 12-13 0.1683000796775076 1107 14-15 0.16984234455091496 1107 16-17 0.27849088674727795 1107 18-19 0.2732612262559613 1107 20-21 0.1274469983374047 1107 22-23 0.2484556227319814 1107 24-25 0.1299327297935875 1107 26-27 0.16144318069130037 1107 28-29 0.21461094308159545 1107 30-31 0.11124693317199785 1107 32-33 0.05375688872336326 1107 34-35 0.12892427641294546 1107 36-37 0.1386023136531307 1107 38-39 0.06254096141791621 1107 40-41 0.06877280347531922 1107 42-43 0.035971651203432486 1107 44-45 0.30647681799373316 1107 46-47 0.3609074338888121 1107 48-49 0.19537220059074656 1107 50-51 0.09569281439514299 1107 52-53 0.008831165422236609 1107 54-55 -0.0022178049118011245 1107 56-57 0.21217186786367392 1107 58-59 0.3790072584827584 1107 60-61 0.2654800271368316 1107 62-63 0.14231340583321384 1107 64-65 0.17797175742175853 1107 66-67 0.0417654949769215 1107 68-69 0.17654690530522998 1107 70-71 0.28836483202768903 1107 72-73 0.1126732203589853 1107 74-75 0.21229427432566794 1107 76-77 0.06994662917123406 1107 78-79 0.09026316856363437 1107 80-81 -0.032206595229848034 1107 82-83 0.04107733590929641 1107 84-85 0.16160561118341477 1107 86-87 0.0584407554838009 1107 88-89 0.19184933981246743 1107 90-91 0.19228895467028195 1107 92-93 0.23178731310593648 1107 94-95 0.23541000651314192 1107 96-97 0.3163810948897563 1107 98-99 0.45221347262311085 1107 100-101 0.8984980091064614 1108 1 0.2025506272193347 1108 2 0.2364825524866454 1108 3 0.06474802936018875 1108 4 0.16209884497519056 1108 5 0.2037000519940264 1108 6 0.20258401169706985 1108 7 0.2217848365332742 1108 8 0.20009345660593425 1108 9 0.23963816816456074 1108 10-11 0.27732226518856606 1108 12-13 0.31405453626788926 1108 14-15 0.42193953015984675 1108 16-17 0.5475149182621735 1108 18-19 0.5386121457772148 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50-51 -2.7334374345315524 2316 52-53 -3.177368123172485 2316 54-55 -2.9267963555931686 2316 56-57 -2.5970483542208243 2316 58-59 -2.312782580347722 2316 60-61 -2.1883957346493403 2316 62-63 -2.6783118188716593 2316 64-65 -2.7088419719052794 2316 66-67 -2.4793564839727935 2316 68-69 -2.6718054466422316 2316 70-71 -2.495854108023309 2316 72-73 -2.3125295480143713 2316 74-75 -2.421722807107148 2316 76-77 -2.4190710437187803 2316 78-79 -2.215020326504469 2316 80-81 -2.177273083674468 2316 82-83 -2.1546898548104743 2316 84-85 -2.249183703572239 2316 86-87 -2.2179221345463915 2316 88-89 -2.353739166615245 2316 90-91 -2.70823490143707 2316 92-93 -2.5308543515961865 2316 94-95 -2.424783779249424 2316 96-97 -2.616215330549551 2316 98-99 -2.86329311116571 2316 100-101 -2.9860737205051784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21444.0 3 5198.0 4 4816.0 5 5534.0 6 6394.0 7 7812.0 8 9566.0 9 11142.0 10 13611.0 11 16390.0 12 19768.0 13 23264.0 14 24713.0 15 26267.0 16 30326.0 17 35845.0 18 33100.0 19 27562.0 20 28920.0 21 32049.0 22 35860.0 23 40672.0 24 46316.0 25 52906.0 26 61608.0 27 71240.0 28 82952.0 29 97738.0 30 115336.0 31 137999.0 32 168217.0 33 209950.0 34 271504.0 35 371525.0 36 539433.0 37 781002.0 38 692734.0 39 104949.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42795522338588 19.5102067106622 14.529432520662722 23.5324055452892 2 15.019762288969 20.78456944697526 40.450395151852156 23.745273112203588 3 18.397058913336874 24.930416486395455 30.81331794392638 25.859206656341293 4 12.664932991294444 17.007590210800053 38.21265504751953 32.11482175038597 5 13.49402295604083 37.510171654527255 34.8860668952386 14.109738494193316 6 30.910973819200443 36.619718971502316 18.31072855403714 14.158578655260106 7 27.360689471882228 33.114544381721075 21.90569155974543 17.619074586651255 8 25.419385252566464 35.114761063677186 20.91389606690612 18.551957616850235 9 26.210600331737254 16.610528020998288 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22.250359245178785 34-35 22.392077000588877 27.028515259874165 28.630941443763398 21.948466295773564 36-37 22.39823591941744 26.82490090403481 28.64610369245517 22.13075948409257 38-39 22.14698485441234 27.083946383031293 28.713445716790158 22.055623045766207 40-41 22.308149288057432 26.93393235548824 28.64413998045941 22.113778375994922 42-43 22.153956857047426 26.916066112509295 28.489909595975583 22.4400674344677 44-45 22.226242408026188 27.03486312973465 28.400859511802757 22.338034950436402 46-47 22.289378994080856 27.12925569024695 28.089700162043087 22.491665153629107 48-49 22.294571760329934 27.159476004488326 28.08663443302905 22.459317802152693 50-51 22.209987091328877 27.294631327340667 28.031598882903914 22.46378269842654 52-53 22.347270797486622 27.300921963389076 27.865129241160453 22.48667799796385 54-55 22.23099565844351 27.14642509408824 27.96533580046021 22.657243447008042 56-57 22.18002572340609 27.379885670613334 27.830104103085635 22.609984502894942 58-59 22.29007139748521 27.059493983556234 27.95869061481821 22.69174400414035 60-61 22.367864221113386 27.00397381830496 28.12289851800506 22.50526344257659 62-63 22.168302515245884 27.014875304908863 28.15355863986791 22.663263539977347 64-65 22.07799927143085 26.993216303235425 28.07510029595915 22.853684129374578 66-67 22.052248341174433 27.118847961519933 27.89312938821762 22.935774309088014 68-69 22.075419993328037 26.951978128023015 28.021292830090776 22.951309048558173 70-71 22.069343577786398 26.750646006998725 28.04490831597464 23.135102099240235 72-73 22.04352016286222 26.73351839262073 28.015483963307037 23.207477481210017 74-75 21.93331603968563 27.001006076002287 27.822803510579096 23.242874373732988 76-77 21.94038674899161 26.774749144122158 27.95683259533041 23.32803151155582 78-79 21.96829587380468 26.776739326857918 27.951867329267376 23.303097470070025 80-81 21.925353534058072 26.73961982918163 27.854778620795063 23.48024801596523 82-83 21.97275967341544 26.703703023816043 27.94862308628622 23.3749142164823 84-85 21.927506799782446 26.54066231069516 27.935532625893938 23.596298263628455 86-87 21.76953475934885 26.412939687627414 28.16620099652821 23.651324556495528 88-89 21.956304342146854 26.26956129002193 28.065395448080665 23.70873891975055 90-91 21.85227710768923 26.312812831958666 28.136252754981033 23.698657305371075 92-93 21.899491643724524 26.18056005112988 28.10428660041596 23.81566170472963 94-95 21.760750550686268 26.057698430981148 28.155668028084673 24.02588299024791 96-97 21.80137419821663 25.83071707692777 28.23651154093667 24.13139718391893 98-99 21.741718503877227 25.809480918499734 28.120255579832442 24.328544997790598 100-101 21.984157356047852 25.297629134234832 28.502414265110694 24.21579924460662 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3333.0 1 2231.5 2 1055.5 3 994.5 4 991.0 5 1055.0 6 1330.0 7 1953.0 8 2765.5 9 3166.0 10 3007.0 11 2907.0 12 3064.0 13 3325.5 14 3656.5 15 3952.0 16 4345.5 17 4769.5 18 5125.5 19 5534.5 20 6144.5 21 7141.5 22 8335.5 23 10031.5 24 12388.5 25 15697.0 26 21000.5 27 28100.0 28 34775.0 29 41705.5 30 49797.0 31 58947.0 32 67982.0 33 76331.0 34 86304.0 35 96508.0 36 107981.0 37 118444.5 38 124544.5 39 128410.0 40 132239.5 41 137212.5 42 142458.5 43 149716.5 44 156572.0 45 160417.0 46 162761.5 47 165393.5 48 171099.5 49 171412.0 50 164497.0 51 158186.5 52 151467.0 53 144531.0 54 136847.5 55 125923.5 56 113327.5 57 99703.0 58 88932.5 59 79300.5 60 66868.5 61 54705.5 62 43627.0 63 33398.0 64 24512.0 65 18032.0 66 13317.5 67 10134.0 68 7793.0 69 5910.0 70 4491.0 71 3292.0 72 2536.0 73 2000.5 74 1515.5 75 1172.0 76 874.0 77 559.5 78 359.5 79 269.0 80 199.5 81 148.5 82 109.5 83 80.5 84 66.5 85 53.0 86 37.5 87 29.0 88 26.0 89 23.0 90 18.0 91 12.0 92 8.5 93 4.5 94 5.5 95 6.0 96 5.0 97 3.5 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27559543359277655 2 0.1654369662184607 3 0.19220882613364879 4 0.16170953563482246 5 0.23382007950445335 6 0.19316998747911526 7 0.23738340937057295 8 0.24373645143450987 9 0.14717490065459776 10-11 0.20367243340030988 12-13 0.20911120003807138 14-15 0.17137975648860096 16-17 0.28264004296625644 18-19 0.2301746992795744 20-21 0.19618240779356505 22-23 0.24259946789170198 24-25 0.23178054201529277 26-27 0.33904380387338645 28-29 0.3114162759311368 30-31 0.2862150943121988 32-33 0.19070847671633526 34-35 0.35661430212770645 36-37 0.39589298101378145 38-39 0.3770917273780714 40-41 0.34045038145211787 42-43 0.37315331015762343 44-45 0.3817451548677078 46-47 0.3866798978730906 48-49 0.40593828922188835 50-51 0.3980262903415241 52-53 0.39779186074506884 54-55 0.39234137262748453 56-57 0.371500581502614 58-59 0.34749499082559776 60-61 0.3156711731067993 62-63 0.3808894868406462 64-65 0.41307667043395024 66-67 0.4187733096278125 68-69 0.42305164976312065 70-71 0.42293443496489297 72-73 0.4141198821381761 74-75 0.4099001494019818 76-77 0.39428713827806305 78-79 0.3692969432959348 80-81 0.3795063522215604 82-83 0.389270344913921 84-85 0.39009084850151426 86-87 0.38494511885932187 88-89 0.3934666346904698 90-91 0.41460046281090934 92-93 0.4141902110171127 94-95 0.4224069683728687 96-97 0.42273516980790604 98-99 0.4200978368477847 100-101 0.39292744661862267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4265673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.942468911329556 #Duplication Level Percentage of deduplicated Percentage of total 1 73.923930159487 40.61563234590911 2 14.180930160624792 15.582706289677265 3 5.158815025412354 8.50314102459054 4 2.3503326948178893 5.1653232406525325 5 1.288894743485084 3.5407529686952657 6 0.7306066018681538 2.4084798305731905 7 0.477266281041051 1.8355531465966677 8 0.3379783757936948 1.4855493123797385 9 0.23787016642795147 1.176225680151046 >10 1.1925234406310925 11.712943367929924 >50 0.0798891074130335 3.019974613073973 >100 0.03937750129254521 3.8098905408395902 >500 0.0012428787129215534 0.4280877337792994 >1k 2.142893703754586E-4 0.17716893822755048 >5k 1.285736222252003E-4 0.5385709669243695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9967 0.23365597878693467 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7688 0.18022947375478618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5217 0.12230192047069713 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.688591929104739E-5 2 0.0 0.0 0.0 0.0 4.688591929104739E-5 3 0.0 0.0 0.0 0.0 4.688591929104739E-5 4 0.0 0.0 0.0 0.0 4.688591929104739E-5 5 0.0 0.0 0.0 0.0 4.688591929104739E-5 6 0.0 0.0 0.0 0.0 7.032887893657109E-5 7 0.0 0.0 0.0 0.0 7.032887893657109E-5 8 0.0 0.0 0.0 0.0 7.032887893657109E-5 9 0.0 0.0 0.0 2.3442959645523694E-5 7.032887893657109E-5 10-11 0.0 0.0 0.0 2.3442959645523694E-5 9.377183858209478E-5 12-13 0.0 0.0 0.0 3.516443946828554E-5 1.6410071751866588E-4 14-15 0.0 0.0 0.0 4.688591929104739E-5 1.7582219734142772E-4 16-17 0.0 0.0 0.0 4.688591929104739E-5 1.8754367716418955E-4 18-19 0.0 0.0 0.0 4.688591929104739E-5 1.8754367716418955E-4 20-21 0.0 0.0 0.0 5.860739911380924E-5 2.1098663680971325E-4 22-23 0.0 0.0 0.0 2.461510762779988E-4 2.1098663680971325E-4 24-25 0.0 0.0 0.0 4.219732736194265E-4 2.1098663680971325E-4 26-27 0.0 0.0 0.0 0.0013362486997948507 2.1098663680971325E-4 28-29 0.0 0.0 0.0 0.004969907444851023 2.1098663680971325E-4 30-31 0.0 0.0 0.0 0.019047404711988 2.1098663680971325E-4 32-33 0.0 0.0 0.0 0.04831593982942434 2.1098663680971325E-4 34-35 0.0 0.0 0.0 0.09141582113771965 2.1098663680971325E-4 36-37 0.0 0.0 0.0 0.16139305567960788 2.1098663680971325E-4 38-39 0.0 0.0 0.0 0.26524536690927786 2.3442959645523695E-4 40-41 0.0 0.0 0.0 0.3815458897107209 2.461510762779988E-4 42-43 1.1721479822761847E-5 1.1721479822761847E-5 0.0 0.5085950095096365 2.5787255610076063E-4 44-45 2.3442959645523694E-5 2.3442959645523694E-5 0.0 0.6617127004343746 2.5787255610076063E-4 46-47 2.3442959645523694E-5 2.3442959645523694E-5 0.0 0.8431494866109053 2.6959403592352247E-4 48-49 2.3442959645523694E-5 2.3442959645523694E-5 0.0 1.0358623363769328 2.8131551574628436E-4 50-51 2.3442959645523694E-5 2.3442959645523694E-5 0.0 1.2430043278047802 3.0475847539180803E-4 52-53 2.3442959645523694E-5 2.3442959645523694E-5 0.0 1.4519514271253329 3.0475847539180803E-4 54-55 2.3442959645523694E-5 2.3442959645523694E-5 0.0 1.6886784336258311 3.0475847539180803E-4 56-57 4.688591929104739E-5 4.688591929104739E-5 0.0 1.9621171149312195 3.0475847539180803E-4 58-59 4.688591929104739E-5 4.688591929104739E-5 0.0 2.256525523639529 3.0475847539180803E-4 60-61 4.688591929104739E-5 4.688591929104739E-5 0.0 2.5756193688545745 3.0475847539180803E-4 62-63 4.688591929104739E-5 4.688591929104739E-5 0.0 2.9025431625912255 3.2820143503733176E-4 64-65 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 3.256649068036861 3.6336587450561727E-4 66-67 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 3.648767732547713 3.750873543283791E-4 68-69 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 4.080856643254183 3.750873543283791E-4 70-71 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 4.5630079942836685 3.8680883415114094E-4 72-73 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 5.051254514820991 4.2197327361942656E-4 74-75 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 5.551726538813453 4.5713771308771207E-4 76-77 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 6.090281650750069 4.923021525559976E-4 78-79 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 6.688381880186315 5.157451122015213E-4 80-81 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 7.328632551065213 5.274665920242832E-4 82-83 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 7.987825133337694 6.095169507836161E-4 84-85 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 8.65821172884091 6.095169507836161E-4 86-87 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 9.364348837803554 6.212384306063779E-4 88-89 4.688591929104739E-5 4.688591929104739E-5 2.3442959645523694E-5 10.112073757177356 6.329599104291397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10585 0.0 33.31823 1 GTATCAA 16060 0.0 31.519035 1 ATCAACG 19125 0.0 26.221376 3 CAACGCA 19350 0.0 26.014551 5 TCAACGC 19350 0.0 25.965513 4 TATCAAC 19630 0.0 25.595144 2 AACGCAG 20040 0.0 25.21265 6 GTACATG 14895 0.0 23.294382 1 TACATGG 14955 0.0 22.746506 2 ACGCAGA 22440 0.0 22.326357 7 CGCAGAG 22655 0.0 22.009508 8 ACATGGG 15160 0.0 21.249683 3 GAGTACT 11295 0.0 21.055756 12-13 GCAGAGT 24640 0.0 19.71516 9 AGAGTAC 19125 0.0 19.656551 10-11 AGTACTT 12235 0.0 19.282875 12-13 GTACTTT 12505 0.0 19.018599 14-15 TAGCGCG 135 0.009468467 17.571878 4 CATGGGA 7970 0.0 17.441795 4 CAGAGTA 23695 0.0 16.887077 10-11 >>END_MODULE