FastQCFastQC Report
Thu 26 May 2016
SRR938750_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938750_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265673
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101600.23818046999852074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83590.19595969967693255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54870.12863151957498853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106600.034.277171
GTATCAA160600.031.757781
ATCAACG199300.025.1699643
TCAACGC201000.025.0279184
CAACGCA201700.024.9410575
TATCAAC203250.024.8442542
AACGCAG208650.024.1029386
GTACATG158450.021.499171
ACGCAGA236550.021.1593277
CGCAGAG236800.020.9967448
TACATGG156700.020.988392
TAGATCG1150.00374377520.6341725
ACATGGG159100.020.1646843
AGAGTAC204200.020.09824610-11
GAGTACT117800.020.01745412-13
AGTACTT125400.019.31505812-13
GCAGAGT261100.018.7155559
GTACTTT129750.018.13727614-15
CATGGGG80300.017.6122824
GTATAGG11050.017.2225951
Produced by FastQC (version 0.11.5)