##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938750_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4265673 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05290302374326 33.0 31.0 34.0 30.0 34.0 2 32.26035891640076 34.0 31.0 34.0 30.0 34.0 3 32.34611607593925 34.0 31.0 34.0 30.0 34.0 4 35.972809917684735 37.0 35.0 37.0 35.0 37.0 5 35.8728374162764 37.0 35.0 37.0 35.0 37.0 6 35.88813652617066 37.0 35.0 37.0 35.0 37.0 7 35.8020209237792 37.0 35.0 37.0 33.0 37.0 8 35.78860756555882 37.0 35.0 37.0 33.0 37.0 9 37.434146733704154 39.0 37.0 39.0 34.0 39.0 10-11 37.362380449696914 39.0 37.0 39.0 34.0 39.0 12-13 37.29031386137662 39.0 37.0 39.0 33.5 39.0 14-15 38.52417367669767 40.0 38.0 41.0 34.0 41.0 16-17 38.45426735710871 40.0 38.0 41.0 33.5 41.0 18-19 38.57767766071146 40.0 38.0 41.0 34.0 41.0 20-21 38.5721784112378 40.0 38.0 41.0 34.0 41.0 22-23 38.49843975382079 40.0 38.0 41.0 34.0 41.0 24-25 38.4310554278305 40.0 38.0 41.0 34.0 41.0 26-27 38.26363202242647 40.0 38.0 41.0 33.0 41.0 28-29 38.10375654673952 40.0 38.0 41.0 33.0 41.0 30-31 37.903210700867135 40.0 37.0 41.0 33.0 41.0 32-33 37.782582490500324 40.0 37.0 41.0 33.0 41.0 34-35 37.77389382636691 40.0 37.0 41.0 33.0 41.0 36-37 37.677116600358254 40.0 37.0 41.0 32.5 41.0 38-39 37.56930406995566 40.0 37.0 41.0 32.0 41.0 40-41 37.41661081381531 40.0 37.0 41.0 32.0 41.0 42-43 37.24549279797115 40.0 36.5 41.0 31.0 41.0 44-45 37.030414661414504 40.0 36.0 41.0 31.0 41.0 46-47 36.76929279389208 40.0 36.0 41.0 30.5 41.0 48-49 36.51112638029216 39.0 36.0 41.0 30.0 41.0 50-51 36.30256608980576 39.0 35.0 41.0 30.0 41.0 52-53 36.09992291954869 39.0 35.0 40.5 29.0 41.0 54-55 35.84311397052704 39.0 35.0 40.0 28.5 41.0 56-57 35.586535114154316 38.0 35.0 40.0 28.0 41.0 58-59 35.28881269145572 38.0 34.0 40.0 28.0 41.0 60-61 34.943842272954356 38.0 34.0 40.0 27.0 41.0 62-63 34.59062848933803 37.0 34.0 40.0 26.0 41.0 64-65 34.206601162348825 37.0 33.5 39.5 26.0 41.0 66-67 33.81034258837937 36.0 33.0 39.0 26.0 40.5 68-69 33.48213294830616 36.0 33.0 39.0 25.5 40.0 70-71 33.068904719138104 35.0 32.0 38.5 25.0 40.0 72-73 32.594862287850006 35.0 32.0 37.5 23.5 40.0 74-75 32.104838087682765 35.0 31.0 37.0 22.0 39.0 76-77 30.125563187801784 33.0 29.0 35.0 20.0 37.5 78-79 31.0940294767086 34.0 30.5 36.0 20.0 38.0 80-81 31.154763386691855 34.0 31.0 36.0 20.0 37.5 82-83 30.84382042411596 34.0 31.0 35.0 20.0 37.0 84-85 30.45124520796601 34.0 30.5 35.0 18.0 36.5 86-87 29.88564641499712 34.0 30.0 35.0 12.5 36.0 88-89 29.304401204686812 34.0 29.0 35.0 7.0 36.0 90-91 28.802414765501247 34.0 29.0 35.0 2.0 35.0 92-93 28.52206638905514 34.0 29.0 35.0 2.0 35.0 94-95 28.203189156787218 33.5 29.0 35.0 2.0 35.0 96-97 27.619881434887297 33.0 27.5 35.0 2.0 35.0 98-99 26.82241618145601 33.0 26.5 35.0 2.0 35.0 100-101 23.213444748343345 28.5 12.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05357867015580098 1101 2 0.1148725544685476 1101 3 0.031061593810001398 1101 4 0.08138017735445402 1101 5 -0.08405610387372775 1101 6 0.0012766526347576246 1101 7 0.07511697355897695 1101 8 0.18777577787928834 1101 9 0.2164358933797601 1101 10-11 0.25117704143383435 1101 12-13 0.18206269139238174 1101 14-15 0.22863635753436995 1101 16-17 0.2728294163916445 1101 18-19 0.23765169020855836 1101 20-21 0.18672647564554978 1101 22-23 0.11723073350641755 1101 24-25 0.18357886547035207 1101 26-27 0.07583717253545075 1101 28-29 0.16651565127374113 1101 30-31 0.13584997666440302 1101 32-33 0.2146757367106673 1101 34-35 0.3131132094355493 1101 36-37 0.2294306522263554 1101 38-39 0.38807764371830444 1101 40-41 0.40763243560441254 1101 42-43 0.4113078683934219 1101 44-45 0.2863663588801373 1101 46-47 0.3048439925557318 1101 48-49 0.21071237658910746 1101 50-51 0.16343132465957666 1101 52-53 0.05062588902713827 1101 54-55 0.0714479309006748 1101 56-57 -0.08963666207384335 1101 58-59 -0.031169476633991167 1101 60-61 0.09363731798797659 1101 62-63 0.027811539809029284 1101 64-65 0.05556175861653401 1101 66-67 0.07190224681126267 1101 68-69 0.11787290647206561 1101 70-71 0.1503249423113644 1101 72-73 -0.03277547549738813 1101 74-75 -0.15598113233225774 1101 76-77 -0.1970708979260074 1101 78-79 -0.19626957079726637 1101 80-81 0.003920502435928341 1101 82-83 -0.04658057652590131 1101 84-85 0.059612997815044366 1101 86-87 0.0893568811887242 1101 88-89 0.18497155509055574 1101 90-91 0.18166367471756573 1101 92-93 0.02142309686788124 1101 94-95 0.03187171973437941 1101 96-97 -0.07363822679089438 1101 98-99 -0.1976791386579606 1101 100-101 0.42768071624799475 1102 1 0.25654270757200237 1102 2 0.1823866487244601 1102 3 0.16394759986393836 1102 4 0.05930919234215537 1102 5 -0.0806756079623625 1102 6 -0.038216093308591326 1102 7 0.11332349406316666 1102 8 0.11570346882866289 1102 9 0.23710392620346 1102 10-11 0.20821193891539025 1102 12-13 0.18768536254547996 1102 14-15 0.3432893282624434 1102 16-17 0.29170051488959103 1102 18-19 0.2766659621674421 1102 20-21 0.2743740259192293 1102 22-23 0.2168267462193043 1102 24-25 0.1590006039720251 1102 26-27 0.20244905709175498 1102 28-29 0.24083210254100607 1102 30-31 0.18472425344148746 1102 32-33 0.2984302032849868 1102 34-35 0.30900026491462995 1102 36-37 0.2857789051915489 1102 38-39 0.41300435335270436 1102 40-41 0.41832828732479754 1102 42-43 0.4139385652151262 1102 44-45 0.3339944249463045 1102 46-47 0.3546437457033136 1102 48-49 0.2214563458000285 1102 50-51 0.2171985836166428 1102 52-53 0.22685700540752407 1102 54-55 0.24874053849816846 1102 56-57 0.2935663351966369 1102 58-59 0.2427686813227794 1102 60-61 0.24199822018710648 1102 62-63 0.09185477473941006 1102 64-65 0.24710222231414036 1102 66-67 0.27200007885849686 1102 68-69 0.26880543753016894 1102 70-71 0.3079701421968224 1102 72-73 0.2683337691730969 1102 74-75 0.21056399485733124 1102 76-77 0.23864249069674415 1102 78-79 0.21299980723763667 1102 80-81 0.325824635132399 1102 82-83 0.26717948741311304 1102 84-85 0.41702971741877803 1102 86-87 0.3853834920692414 1102 88-89 0.37355776927392625 1102 90-91 0.5209202838559897 1102 92-93 0.5370042390156371 1102 94-95 0.5758449115692166 1102 96-97 0.5030456695973236 1102 98-99 0.7703955082157314 1102 100-101 1.4533330341165431 1103 1 0.1927343996355475 1103 2 0.22329602391378955 1103 3 0.15351315516678454 1103 4 0.017221191806839897 1103 5 0.0056051757495581 1103 6 -0.01710169772188408 1103 7 0.05802879083481116 1103 8 0.11543747981355779 1103 9 0.14226316375224712 1103 10-11 0.14011550026262398 1103 12-13 0.14403897017181322 1103 14-15 0.18559817505247622 1103 16-17 0.1625031832030075 1103 18-19 0.007169810262681153 1103 20-21 0.20286883706213388 1103 22-23 0.21663969662204607 1103 24-25 0.1376710524428475 1103 26-27 0.16759891511875225 1103 28-29 0.08828242551081189 1103 30-31 0.25009023401605646 1103 32-33 0.27103408269708495 1103 34-35 0.1608203334434819 1103 36-37 0.20214903601176815 1103 38-39 0.11040925451244732 1103 40-41 0.25696219351934246 1103 42-43 0.34278601461751634 1103 44-45 0.2979152273067953 1103 46-47 0.35557936767372667 1103 48-49 0.3744119106751356 1103 50-51 0.4146666017890439 1103 52-53 0.39112573382755755 1103 54-55 0.36590081721875833 1103 56-57 0.25886006203875667 1103 58-59 0.4472006003037805 1103 60-61 0.4519800060096202 1103 62-63 0.38447712666572187 1103 64-65 0.34516405247620696 1103 66-67 0.3753295041536475 1103 68-69 0.3472671050494185 1103 70-71 0.3570131829938319 1103 72-73 0.4586848407267894 1103 74-75 0.684468360903324 1103 76-77 0.6294932683276819 1103 78-79 0.48449243022821165 1103 80-81 0.5563762956593017 1103 82-83 0.6197960845868131 1103 84-85 0.6519100140192542 1103 86-87 0.5375548410075304 1103 88-89 0.5128916816881102 1103 90-91 0.4687972824343305 1103 92-93 0.5794185838498258 1103 94-95 0.7467521746112915 1103 96-97 0.6254971332187047 1103 98-99 0.7394892014222911 1103 100-101 1.1850811849232805 1104 1 0.16674501210151504 1104 2 0.1246267472879552 1104 3 0.14835472072435607 1104 4 0.0930394701995354 1104 5 -0.06428721143969085 1104 6 0.0889758041721933 1104 7 0.08826841705191413 1104 8 0.05812039321247653 1104 9 0.10895280686988684 1104 10-11 0.1604701602793881 1104 12-13 0.10965629733379245 1104 14-15 0.11418736542767505 1104 16-17 0.18386329120833267 1104 18-19 0.1962394955511968 1104 20-21 0.15793237716187747 1104 22-23 0.13080746010598432 1104 24-25 0.16674410372024084 1104 26-27 0.13549452541179363 1104 28-29 0.1301749245231818 1104 30-31 -0.03659586803608761 1104 32-33 0.07534511814247224 1104 34-35 0.08697996885492643 1104 36-37 0.16939553567982557 1104 38-39 0.06660816638311928 1104 40-41 0.15335005257816192 1104 42-43 0.14331550838917195 1104 44-45 0.17274810680580543 1104 46-47 0.19203181305617534 1104 48-49 0.22783732677151391 1104 50-51 0.14910408489910054 1104 52-53 0.1192713026810921 1104 54-55 0.06146265356900926 1104 56-57 0.019015563948080683 1104 58-59 0.19999938335195822 1104 60-61 0.3408258473896524 1104 62-63 0.3600013328415699 1104 64-65 0.22268986427394566 1104 66-67 0.1277122543642335 1104 68-69 0.18746700864433308 1104 70-71 0.119371346236953 1104 72-73 0.29532029763577583 1104 74-75 0.31308017004610633 1104 76-77 0.2670021315807105 1104 78-79 0.16597728878467066 1104 80-81 0.24100612300835778 1104 82-83 0.19529459403544536 1104 84-85 0.14778230072018417 1104 86-87 0.35937813958063103 1104 88-89 0.3344619098552819 1104 90-91 0.3681728519627576 1104 92-93 0.35579685322889887 1104 94-95 0.4446656003606009 1104 96-97 0.5343387275792075 1104 98-99 0.7920395168849126 1104 100-101 1.5414503446713645 1105 1 0.07562299696478192 1105 2 0.062223569803329326 1105 3 0.06536149744736974 1105 4 0.029300056696591525 1105 5 -0.1453739439399797 1105 6 -0.09233054056031875 1105 7 0.01752925605526201 1105 8 0.0637301276713984 1105 9 0.10121847079083324 1105 10-11 0.09977761058001278 1105 12-13 0.028980399400929002 1105 14-15 0.02152124017089818 1105 16-17 0.06274830530090725 1105 18-19 0.08780032568270713 1105 20-21 0.08853764157692012 1105 22-23 0.04608122470186515 1105 24-25 3.296248727622242E-4 1105 26-27 0.030319784230748326 1105 28-29 0.02934738632307443 1105 30-31 0.09132914441860862 1105 32-33 0.07165511397953139 1105 34-35 -0.04031350488099861 1105 36-37 0.03546783437430179 1105 38-39 0.20362845560519105 1105 40-41 0.09053604052610353 1105 42-43 0.2055173632867664 1105 44-45 0.2333169966652875 1105 46-47 0.0675646354623538 1105 48-49 0.05984952896763218 1105 50-51 0.004434765778910332 1105 52-53 0.13845304579347584 1105 54-55 0.19068964307975733 1105 56-57 0.18830656721700478 1105 58-59 0.2528516808180328 1105 60-61 0.14593739375185777 1105 62-63 0.01917925423704503 1105 64-65 0.13322374447676566 1105 66-67 0.16972083957828232 1105 68-69 0.09581991690264857 1105 70-71 0.1885118011577518 1105 72-73 0.25769904343017913 1105 74-75 0.26611522747422356 1105 76-77 0.3228112095232625 1105 78-79 -0.014682366851335615 1105 80-81 0.10687791805513669 1105 82-83 0.1505390133050888 1105 84-85 0.16147865166298558 1105 86-87 0.21361444452633904 1105 88-89 0.15619028132616464 1105 90-91 0.05849626475595571 1105 92-93 0.21731421133059925 1105 94-95 0.3792999098241232 1105 96-97 0.45501953914808624 1105 98-99 0.6600984259212268 1105 100-101 1.2867603473329154 1106 1 0.11634886296199909 1106 2 0.04150081548916518 1106 3 0.03999120716426319 1106 4 0.016614410521327727 1106 5 -0.08139821506777878 1106 6 0.004442802336605212 1106 7 -0.0016065926431068078 1106 8 0.038115965821688746 1106 9 0.024663473111409928 1106 10-11 0.03715483095045613 1106 12-13 0.09100117384082296 1106 14-15 0.02542300064514791 1106 16-17 0.01783524783498791 1106 18-19 -0.09505311404557659 1106 20-21 0.002641160415784327 1106 22-23 0.10387314276439952 1106 24-25 0.06140993266568273 1106 26-27 0.07408629197303185 1106 28-29 0.15008625282659693 1106 30-31 0.01647990282543077 1106 32-33 -0.015906987830518915 1106 34-35 0.0069868199998026626 1106 36-37 0.010516381903940442 1106 38-39 0.1512753903336801 1106 40-41 0.18052736473183018 1106 42-43 0.163036033385076 1106 44-45 0.1629387162129845 1106 46-47 0.03942064892170549 1106 48-49 0.07907912371047132 1106 50-51 0.11035959490003933 1106 52-53 0.11759904701704471 1106 54-55 0.23720044431177456 1106 56-57 0.11465084066538367 1106 58-59 0.07066250631061877 1106 60-61 0.06854535333162204 1106 62-63 0.13651119698207737 1106 64-65 0.22844259072797968 1106 66-67 0.0830280048877654 1106 68-69 0.12771899289101185 1106 70-71 0.17580975272097277 1106 72-73 0.18747845674607788 1106 74-75 0.3909545538739607 1106 76-77 0.39991921656937635 1106 78-79 0.2867634845895317 1106 80-81 0.15426882455697566 1106 82-83 0.25512170496366693 1106 84-85 0.2464029692023324 1106 86-87 0.26853195855528256 1106 88-89 0.47522961510954786 1106 90-91 0.31581114598459337 1106 92-93 0.48311417757674846 1106 94-95 0.5828910030269441 1106 96-97 0.5745803435445289 1106 98-99 0.8027358937495883 1106 100-101 1.3674207888754673 1107 1 0.0011093127430896743 1107 2 -0.002278893276994154 1107 3 -0.02598957572349292 1107 4 -0.0781674404583299 1107 5 -0.0830397343142053 1107 6 -0.11016101378476861 1107 7 0.0107954040204703 1107 8 0.05531135361425754 1107 9 0.03186476078438716 1107 10-11 -0.07586765810398788 1107 12-13 -0.023163448734322856 1107 14-15 -0.0016954604952061914 1107 16-17 -0.03526027369937168 1107 18-19 -0.0241787069647188 1107 20-21 -0.10161650984747439 1107 22-23 -0.09407841791936988 1107 24-25 -0.09075511198749808 1107 26-27 -0.0596111115771194 1107 28-29 -0.09275794385170855 1107 30-31 -0.04374132594085722 1107 32-33 0.00809079667854462 1107 34-35 -8.379539707163985E-4 1107 36-37 -0.08173441409837778 1107 38-39 -0.08648099694635647 1107 40-41 -0.19459621242972958 1107 42-43 -0.08906598220501394 1107 44-45 -0.051797721599172064 1107 46-47 -0.11691450992702812 1107 48-49 -0.07080835355258586 1107 50-51 -0.09542694625424275 1107 52-53 -0.19170924957986557 1107 54-55 -0.17719287714083976 1107 56-57 -0.08916841069882508 1107 58-59 -0.039961350561377174 1107 60-61 -0.01555777691294935 1107 62-63 -0.09246367471960326 1107 64-65 -0.06997940062612429 1107 66-67 -0.1313425691551089 1107 68-69 -0.03294618198663812 1107 70-71 -0.12953617121117844 1107 72-73 -0.11029534334637958 1107 74-75 0.02116829577993684 1107 76-77 0.03182043314230398 1107 78-79 0.14724183341022368 1107 80-81 0.19387606378142053 1107 82-83 0.17140532255762153 1107 84-85 0.15090456958153098 1107 86-87 0.027558901202919373 1107 88-89 -0.022819844228592956 1107 90-91 0.17613597857705798 1107 92-93 0.4225137380796262 1107 94-95 0.3779111941009994 1107 96-97 0.5289595392011428 1107 98-99 0.7045914982591235 1107 100-101 1.3510617583317561 1108 1 0.21208237007887476 1108 2 0.08973613297671079 1108 3 0.08985100501673315 1108 4 0.08420963330978282 1108 5 0.06907394856444427 1108 6 0.07269106781188839 1108 7 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-1.9002916629553894 2316 98-99 -1.9475514428457963 2316 100-101 -1.9323071306845314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 42.0 5 87.0 6 203.0 7 567.0 8 1391.0 9 2586.0 10 4365.0 11 6401.0 12 9225.0 13 12497.0 14 14645.0 15 18705.0 16 23746.0 17 31232.0 18 29713.0 19 22195.0 20 21659.0 21 23259.0 22 25451.0 23 28587.0 24 33617.0 25 40546.0 26 49248.0 27 60618.0 28 74275.0 29 91077.0 30 111475.0 31 135110.0 32 166248.0 33 210173.0 34 276456.0 35 382053.0 36 577340.0 37 910847.0 38 793594.0 39 76411.0 40 4.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.18317436692866 20.386627788804766 15.047274010678802 23.382923833587775 2 15.412292503433807 21.41941025484138 39.539622469889274 23.628674771835534 3 18.34596791643429 25.175347477408604 31.088482403597272 25.39020220255983 4 13.065019283006457 17.73305173650207 37.69412235771472 31.50780662277676 5 13.351287847521856 37.824775822609084 34.91923986042847 13.904696469440589 6 30.932846703007648 37.18844390513335 17.8784130624983 14.000296329360706 7 27.340468901390235 33.316993590460406 21.675337045291563 17.667200462857796 8 25.385583939509665 35.749622626957105 20.538611375039764 18.32618205849347 9 25.908596369201298 16.753886198027836 20.959529715475142 36.377987717295724 10-11 24.356602111788693 26.85895051027118 28.260440966759525 20.524006411180604 12-13 25.100259677663995 24.40689898170816 28.43264122683572 22.060200113792124 14-15 22.377617787392516 25.817848672413472 26.587727188652295 25.216806351541717 16-17 21.804453638525995 28.859927896686617 27.20773612569176 22.127882339095628 18-19 21.670578124483523 27.740687108458616 29.244775677835595 21.343959089222263 20-21 22.67313973668399 26.900315612565706 29.007474318823785 21.41907033192652 22-23 22.27437499311363 26.90595364436046 28.79096686501755 22.028704497508365 24-25 22.143756938418434 27.044438581070207 28.998817648370945 21.812986832140414 26-27 22.075369498605298 27.13799650999628 28.901975719073334 21.88465827232508 28-29 21.885046040800596 27.34507544296058 28.933300794505346 21.836577721733473 30-31 22.190074110228327 27.01100154653205 28.85120354982672 21.947720793412902 32-33 21.98132627606476 27.13556571260854 28.743037734022277 22.140070277304424 34-35 22.362270221784172 27.182375802481523 28.653997326839477 21.801356648894828 36-37 22.424186790926342 26.940663519377004 28.657053634641244 21.978096055055413 38-39 22.16420228810311 27.186141958990458 28.647501058004433 22.002154694902 40-41 22.359809616652 27.013784997921597 28.61855316251724 22.007852222909165 42-43 22.212024988523716 27.002066434437012 28.47824007494869 22.307668502090582 44-45 22.253471531524962 27.111652246568497 28.380103558385017 22.25477266352152 46-47 22.357860233498208 27.197013439708854 28.031952040993584 22.41317428579935 48-49 22.37067593272884 27.238148331700895 28.01962067722211 22.371555058348154 50-51 22.25274235806222 27.385192766813766 27.940111194672255 22.421953680451757 52-53 22.406933208429244 27.4272195735585 27.74627825433408 22.41956896367818 54-55 22.256581786742675 27.278942853800558 27.804580894972492 22.659894464484267 56-57 22.221839315700723 27.514353719823546 27.681691684137753 22.582115280337977 58-59 22.324091559796464 27.24405744072503 27.82214056733916 22.609710432139348 60-61 22.32574762645801 27.279955344730332 27.888878052760173 22.505418976051477 62-63 22.156315622595244 27.229947727564436 27.944038779712038 22.669697870128285 64-65 22.03645064953302 27.22153233669425 27.863926858641086 22.87809015513164 66-67 22.02234726064306 27.313073701931884 27.62396744348684 23.040611593938216 68-69 22.074463315444337 27.14824150790353 27.735888312645823 23.041406864006312 70-71 22.030690390190983 27.01932347047678 27.707973019462955 23.24201311986928 72-73 22.050518171458524 26.993548263075954 27.63865162660148 23.317281938864042 74-75 21.88207247939003 27.245364954588442 27.464956135682826 23.407606430338703 76-77 21.867522805498208 27.010348985491202 27.678619075024308 23.443509133986282 78-79 21.88986657504747 27.026754831752438 27.59658897698537 23.486789616214722 80-81 21.80863508704479 26.986323207411818 27.505227844955716 23.699813860587675 82-83 21.853253495147726 26.992766364061747 27.53623850931211 23.617741631478413 84-85 21.7958573008292 26.868726224443364 27.47003813935105 23.865378335376384 86-87 21.604298492835785 26.731486997427613 27.68221360365689 23.98200090607971 88-89 21.69219753859567 26.688170558120554 27.572635917795722 24.04699598548805 90-91 21.58739120218082 26.68615807099815 27.59019383188481 24.136256894936217 92-93 21.57518530878233 26.628353635629548 27.551823572981426 24.2446374826067 94-95 21.414261139302646 26.550626842914415 27.575473631056564 24.459638386726372 96-97 21.396728320543428 26.353829417358615 27.647026486000964 24.602415776096993 98-99 21.23867675745422 26.400605484761723 27.498685436037878 24.862032321746184 100-101 21.369855274211698 25.966773634351032 27.918999319793837 24.744371771643433 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 556.0 1 395.5 2 304.5 3 371.0 4 347.5 5 375.0 6 628.0 7 1253.0 8 1986.5 9 2374.5 10 2263.0 11 2148.0 12 2344.5 13 2665.5 14 2968.0 15 3272.0 16 3581.0 17 3941.5 18 4343.5 19 4850.5 20 5681.0 21 6693.0 22 7931.0 23 9664.0 24 12108.0 25 15432.5 26 20715.5 27 27663.5 28 34446.5 29 41747.5 30 49583.0 31 58982.5 32 68303.0 33 76346.0 34 86670.5 35 96715.0 36 107583.5 37 118472.5 38 125342.5 39 129481.0 40 133038.0 41 137605.5 42 143094.0 43 151350.0 44 158744.0 45 162291.0 46 164378.0 47 167930.5 48 174258.5 49 174165.5 50 166769.0 51 160463.0 52 153101.0 53 145698.5 54 138443.5 55 127675.5 56 114532.5 57 100019.0 58 89121.5 59 79361.5 60 66017.5 61 53420.5 62 42750.0 63 32260.5 64 23397.0 65 17177.0 66 12463.0 67 9299.5 68 6984.0 69 5195.5 70 3905.5 71 2819.5 72 2117.0 73 1647.5 74 1231.0 75 935.5 76 642.0 77 384.0 78 249.0 79 161.5 80 102.5 81 67.0 82 45.0 83 37.0 84 28.0 85 15.0 86 7.0 87 6.0 88 4.5 89 3.0 90 2.5 91 2.5 92 1.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2333043343922518 2 0.0 3 0.0 4 0.0 5 0.02796745085710977 6 0.0034930009871830306 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029772558749815094 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.040236323787594E-4 26-27 0.0014417420181997073 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007267317490112345 36-37 0.01719541089999163 38-39 0.013315601078657459 40-41 0.0090020965038811 42-43 0.008322250674160913 44-45 0.0036688231845244585 46-47 7.032887893657109E-4 48-49 0.0016644501348321824 50-51 2.3442959645523697E-5 52-53 0.0 54-55 0.0 56-57 0.0012659198208582795 58-59 5.509095516698069E-4 60-61 0.0025083966820710355 62-63 9.494398656437097E-4 64-65 0.001641007175186659 66-67 0.005356716279002164 68-69 3.6336587450561727E-4 70-71 1.2893627805038034E-4 72-73 0.0 74-75 4.4541623326495024E-4 76-77 0.05864256355327753 78-79 0.1409507948687112 80-81 0.0012424768612127558 82-83 0.0010549331840485662 84-85 0.0 86-87 0.1045204355795674 88-89 0.35092938441366694 90-91 0.38562496468904206 92-93 5.274665920242831E-4 94-95 2.578725561007607E-4 96-97 1.0549331840485663E-4 98-99 0.0 100-101 0.0012776413006810414 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4265673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.516839585435605 #Duplication Level Percentage of deduplicated Percentage of total 1 71.89595940305314 37.75748566811133 2 15.2220811659499 15.988311894973432 3 5.460994042925642 8.603824443880363 4 2.527852498027574 5.310192965382271 5 1.4358969309756446 3.770438439263362 6 0.8120815657721652 2.5588774391967704 7 0.5377237935453938 1.976768794482674 8 0.3739535234077069 1.5711085760968788 9 0.2663240873008196 1.2587849433463223 >10 1.330002533833025 12.513262435304414 >50 0.09111787892876583 3.2838093021752064 >100 0.04382110291020226 4.0162698774305 >500 0.0017889579443990773 0.5993096857430842 >1k 2.683436172439216E-4 0.2277122610034483 >5k 8.94478724146376E-5 0.3252092796135817 >10k+ 4.47239362073188E-5 0.2386339939963881 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10160 0.23818046999852074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8359 0.19595969967693255 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5487 0.12863151957498853 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3442959645523694E-5 2 0.0 0.0 0.0 0.0 2.3442959645523694E-5 3 0.0 0.0 0.0 0.0 2.3442959645523694E-5 4 0.0 0.0 0.0 0.0 2.3442959645523694E-5 5 0.0 0.0 0.0 0.0 2.3442959645523694E-5 6 0.0 0.0 0.0 0.0 2.3442959645523694E-5 7 0.0 0.0 0.0 0.0 2.3442959645523694E-5 8 0.0 0.0 0.0 0.0 4.688591929104739E-5 9 0.0 0.0 0.0 2.3442959645523694E-5 4.688591929104739E-5 10-11 0.0 0.0 0.0 2.3442959645523694E-5 4.688591929104739E-5 12-13 0.0 0.0 0.0 2.3442959645523694E-5 9.377183858209478E-5 14-15 0.0 0.0 0.0 2.3442959645523694E-5 1.6410071751866588E-4 16-17 2.3442959645523694E-5 0.0 0.0 2.3442959645523694E-5 2.3442959645523695E-4 18-19 2.3442959645523694E-5 0.0 0.0 2.3442959645523694E-5 2.3442959645523695E-4 20-21 2.3442959645523694E-5 0.0 0.0 3.516443946828554E-5 2.3442959645523695E-4 22-23 2.3442959645523694E-5 0.0 0.0 2.461510762779988E-4 2.3442959645523695E-4 24-25 2.3442959645523694E-5 0.0 0.0 4.1025179379666467E-4 2.3442959645523695E-4 26-27 2.3442959645523694E-5 0.0 0.0 0.001324527219972089 2.5787255610076063E-4 28-29 2.3442959645523694E-5 0.0 0.0 0.005145729642192451 2.5787255610076063E-4 30-31 2.3442959645523694E-5 0.0 0.0 0.019481099465430193 2.5787255610076063E-4 32-33 2.3442959645523694E-5 0.0 0.0 0.04945292337223224 2.5787255610076063E-4 34-35 2.3442959645523694E-5 0.0 0.0 0.09368978822333544 3.0475847539180803E-4 36-37 2.3442959645523694E-5 0.0 0.0 0.16509704330360064 3.0475847539180803E-4 38-39 2.3442959645523694E-5 0.0 0.0 0.2710709423811905 3.0475847539180803E-4 40-41 2.3442959645523694E-5 0.0 0.0 0.3887077138824284 3.164799552145699E-4 42-43 2.3442959645523694E-5 0.0 0.0 0.517679156372277 3.5164439468285543E-4 44-45 2.3442959645523694E-5 0.0 0.0 0.6727543344274163 3.6336587450561727E-4 46-47 2.3442959645523694E-5 0.0 0.0 0.8571214905596374 3.8680883415114094E-4 48-49 2.3442959645523694E-5 0.0 0.0 1.0526123310436595 3.9853031397390283E-4 50-51 2.3442959645523694E-5 0.0 0.0 1.262954286463121 4.219732736194265E-4 52-53 2.3442959645523694E-5 0.0 0.0 1.4743160106271624 4.4541623326495024E-4 54-55 2.3442959645523694E-5 0.0 0.0 1.7141960951999837 4.5713771308771207E-4 56-57 4.688591929104739E-5 0.0 0.0 1.9910457271337958 4.688591929104739E-4 58-59 4.688591929104739E-5 0.0 0.0 2.289638704138831 4.688591929104739E-4 60-61 4.688591929104739E-5 0.0 0.0 2.613983772314474 4.688591929104739E-4 62-63 4.688591929104739E-5 0.0 0.0 2.9459478023749126 4.688591929104739E-4 64-65 4.688591929104739E-5 2.3442959645523694E-5 0.0 3.3057855114538786 4.688591929104739E-4 66-67 4.688591929104739E-5 4.688591929104739E-5 0.0 3.7043627113470725 4.688591929104739E-4 68-69 4.688591929104739E-5 4.688591929104739E-5 0.0 4.141878667211481 4.688591929104739E-4 70-71 4.688591929104739E-5 4.688591929104739E-5 0.0 4.630769869139055 4.8058067273323575E-4 72-73 4.688591929104739E-5 4.688591929104739E-5 0.0 5.12641264344454 5.157451122015213E-4 74-75 4.688591929104739E-5 4.688591929104739E-5 0.0 5.634949045555063 5.39188071847045E-4 76-77 4.688591929104739E-5 4.688591929104739E-5 0.0 6.18200223036318 5.39188071847045E-4 78-79 5.860739911380924E-5 4.688591929104739E-5 0.0 6.788612254150752 5.39188071847045E-4 80-81 9.377183858209478E-5 4.688591929104739E-5 0.0 7.435555890008446 5.509095516698069E-4 82-83 9.377183858209478E-5 4.688591929104739E-5 0.0 8.103938112461973 5.860739911380924E-4 84-85 9.377183858209478E-5 4.688591929104739E-5 0.0 8.784745103527626 5.860739911380924E-4 86-87 9.377183858209478E-5 4.688591929104739E-5 0.0 9.500786862940503 6.095169507836161E-4 88-89 9.377183858209478E-5 4.688591929104739E-5 1.1721479822761847E-5 10.259553416307345 6.329599104291397E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10660 0.0 34.27717 1 GTATCAA 16060 0.0 31.75778 1 ATCAACG 19930 0.0 25.169964 3 TCAACGC 20100 0.0 25.027918 4 CAACGCA 20170 0.0 24.941057 5 TATCAAC 20325 0.0 24.844254 2 AACGCAG 20865 0.0 24.102938 6 GTACATG 15845 0.0 21.49917 1 ACGCAGA 23655 0.0 21.159327 7 CGCAGAG 23680 0.0 20.996744 8 TACATGG 15670 0.0 20.98839 2 TAGATCG 115 0.003743775 20.634172 5 ACATGGG 15910 0.0 20.164684 3 AGAGTAC 20420 0.0 20.098246 10-11 GAGTACT 11780 0.0 20.017454 12-13 AGTACTT 12540 0.0 19.315058 12-13 GCAGAGT 26110 0.0 18.715555 9 GTACTTT 12975 0.0 18.137276 14-15 CATGGGG 8030 0.0 17.612282 4 GTATAGG 1105 0.0 17.222595 1 >>END_MODULE