FastQCFastQC Report
Thu 26 May 2016
SRR938749_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938749_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527691
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123460.34997396313906176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.25858273868091053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63670.1804863294432534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA182750.030.2756121
GGTATCA124600.028.3857421
GTACATG145100.025.388271
ATCAACG223300.024.6784423
TATCAAC223750.024.6711642
TCAACGC223950.024.5856554
TACATGG147250.024.5525112
CAACGCA226850.024.2922445
AACGCAG231200.023.8599246
ACATGGG151600.023.316663
GAGTACT119500.021.53832212-13
ACGCAGA255400.021.433177
CGCAGAG258250.021.1599668
CATGGGG59600.020.5911314
GCAGAGT266650.020.1035529
AGTACTT126850.020.08500912-13
AGAGTAC208950.019.50187910-11
CATGGGA89300.019.2611354
GTACTTT137950.018.572814-15
CAGAGTA260950.016.53213910-11
Produced by FastQC (version 0.11.5)