##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938749_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3527691 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.403654118232012 31.0 31.0 34.0 28.0 34.0 2 31.476878785585246 33.0 31.0 34.0 28.0 34.0 3 31.486886181357722 33.0 31.0 34.0 28.0 34.0 4 35.048060331814774 37.0 35.0 37.0 32.0 37.0 5 35.087504262703284 37.0 35.0 37.0 32.0 37.0 6 35.16485996080723 37.0 35.0 37.0 32.0 37.0 7 35.06900377612438 37.0 35.0 37.0 32.0 37.0 8 35.03706447078273 37.0 35.0 37.0 32.0 37.0 9 36.57271541073184 39.0 37.0 39.0 32.0 39.0 10-11 36.46088631345546 39.0 37.0 39.0 32.0 39.0 12-13 36.339679127225146 39.0 37.0 39.0 32.0 39.0 14-15 37.45221477731468 40.0 37.0 41.0 32.0 41.0 16-17 37.387709836264 40.0 37.0 41.0 32.0 41.0 18-19 37.32927232005298 40.0 37.0 41.0 31.0 41.0 20-21 37.220760406736304 40.0 37.0 41.0 31.0 41.0 22-23 37.12319928247684 40.0 36.5 41.0 31.0 41.0 24-25 37.125763991233924 40.0 37.0 41.0 31.0 41.0 26-27 37.03267930779651 40.0 36.0 41.0 30.5 41.0 28-29 36.91520062273028 40.0 36.0 41.0 30.0 41.0 30-31 36.7064679134312 39.0 36.0 41.0 30.0 41.0 32-33 36.50196048916983 39.0 35.5 41.0 30.0 41.0 34-35 36.17224524483579 39.0 35.0 41.0 29.5 41.0 36-37 35.9187997191364 39.0 35.0 41.0 27.5 41.0 38-39 35.75283946354712 39.0 35.0 41.0 27.0 41.0 40-41 35.578898066752444 39.0 35.0 40.0 27.0 41.0 42-43 35.31593441715842 38.0 35.0 40.0 26.0 41.0 44-45 35.26414232425685 39.0 35.0 40.5 25.0 41.0 46-47 35.07900238427912 39.0 35.0 41.0 24.5 41.0 48-49 34.84968368828223 39.0 34.0 41.0 24.0 41.0 50-51 33.71967952975473 38.0 32.5 39.5 22.0 40.5 52-53 33.83064559792794 38.0 33.0 39.5 23.0 40.5 54-55 34.368532703119406 38.0 34.0 40.0 23.0 41.0 56-57 34.290876099975875 38.0 34.0 40.0 22.5 41.0 58-59 34.08266852170442 38.0 34.0 40.0 22.0 41.0 60-61 33.80603544924995 38.0 33.0 40.0 20.5 41.0 62-63 33.45313124080312 37.0 33.0 40.0 19.5 41.0 64-65 33.065057852289215 37.0 32.5 40.0 18.0 41.0 66-67 32.67532672221007 36.0 32.0 39.0 15.5 41.0 68-69 32.267695214801975 36.0 31.5 39.0 13.0 41.0 70-71 31.86340271866215 35.0 31.0 39.0 9.5 40.0 72-73 31.43963459384623 35.0 31.0 38.0 7.5 40.0 74-75 30.97609980579365 35.0 31.0 37.0 6.5 39.0 76-77 30.52687409413126 35.0 30.0 37.0 2.0 39.0 78-79 30.09994568685296 34.5 30.0 36.0 2.0 39.0 80-81 29.653821579044198 34.0 29.0 36.0 2.0 37.5 82-83 29.18466285737611 34.0 29.0 35.0 2.0 37.0 84-85 28.770273813664517 34.0 29.0 35.0 2.0 36.5 86-87 28.375825291954428 34.0 29.0 35.0 2.0 36.0 88-89 27.999768262016147 34.0 27.5 35.0 2.0 36.0 90-91 27.582154446066845 34.0 26.5 35.0 2.0 35.5 92-93 27.127341510353375 34.0 26.0 35.0 2.0 35.0 94-95 26.606611661849065 33.0 25.0 35.0 2.0 35.0 96-97 26.010805368157243 33.0 24.0 35.0 2.0 35.0 98-99 25.293249890650856 33.0 19.0 35.0 2.0 35.0 100-101 23.125083092595126 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3986868784614508 1101 2 0.24965533158334097 1101 3 0.4179819038520307 1101 4 0.38020404473370917 1101 5 0.1512597090936154 1101 6 0.06612177467631142 1101 7 0.1793800961725438 1101 8 0.2312902099006493 1101 9 0.4031242162665194 1101 10-11 0.40821882516064534 1101 12-13 0.3632060053992774 1101 14-15 0.6127898111357908 1101 16-17 0.5966637784243787 1101 18-19 0.6126418037911847 1101 20-21 0.6547641207661599 1101 22-23 0.6707392441042472 1101 24-25 0.6223398679685559 1101 26-27 0.6198055557532882 1101 28-29 0.6090390213805961 1101 30-31 0.42543477507614114 1101 32-33 0.37051619923915524 1101 34-35 0.41305616602015505 1101 36-37 0.5581866890731035 1101 38-39 0.4745481780907994 1101 40-41 0.5036189070982644 1101 42-43 0.4665222124112347 1101 44-45 0.7958480574164284 1101 46-47 0.7748151618048951 1101 48-49 0.7403497104296903 1101 50-51 0.5100114430498124 1101 52-53 0.5083124880642345 1101 54-55 0.4700077621232879 1101 56-57 0.5749943069699768 1101 58-59 0.5775527968620224 1101 60-61 0.4607911875568149 1101 62-63 0.47944959685676025 1101 64-65 0.4647313089081635 1101 66-67 0.4350302500533303 1101 68-69 0.46535725863794397 1101 70-71 0.2796079649738239 1101 72-73 0.2660068399916966 1101 74-75 0.16582959777084127 1101 76-77 0.1371323224761163 1101 78-79 0.1455408239250069 1101 80-81 0.11800740845471225 1101 82-83 0.03792353378909752 1101 84-85 0.0033742621112935467 1101 86-87 -0.02101092515164993 1101 88-89 -0.009432522667875531 1101 90-91 -0.14953606142170983 1101 92-93 -0.20855583638125452 1101 94-95 -0.32110885987072635 1101 96-97 -0.5687651962204221 1101 98-99 -0.541368152672792 1101 100-101 -0.3829969678605458 1102 1 0.4185787180940679 1102 2 0.44046874991420637 1102 3 0.5265481243823622 1102 4 0.4260020043900923 1102 5 0.3894068837313185 1102 6 0.4241748880634191 1102 7 0.5257430275803401 1102 8 0.4952558886220615 1102 9 0.6943263673889106 1102 10-11 0.5119878212028226 1102 12-13 0.6277467266698125 1102 14-15 0.5622751933729191 1102 16-17 0.6676120447365506 1102 18-19 0.7768204798841225 1102 20-21 0.718664842842351 1102 22-23 0.7182019066217435 1102 24-25 0.7185426736927951 1102 26-27 0.712209153042231 1102 28-29 0.7081915637534735 1102 30-31 0.7296222127630827 1102 32-33 0.7453432332885228 1102 34-35 0.6877517761594305 1102 36-37 0.5948020953794426 1102 38-39 0.6811042673986378 1102 40-41 0.6681787628844447 1102 42-43 0.6997910926392308 1102 44-45 0.824399775998053 1102 46-47 0.7152841588541463 1102 48-49 0.7266514113874223 1102 50-51 0.5782154169865876 1102 52-53 0.5307507288972957 1102 54-55 0.5843239046049291 1102 56-57 0.5474360725822294 1102 58-59 0.5146093041079567 1102 60-61 0.5325964787539235 1102 62-63 0.36126223973619886 1102 64-65 0.35718928850472764 1102 66-67 0.3779963013942549 1102 68-69 0.28354109097930547 1102 70-71 0.21012651034780205 1102 72-73 0.12121696718587316 1102 74-75 0.16981209472442416 1102 76-77 3.754214619853258E-4 1102 78-79 -0.05799604023000171 1102 80-81 -9.750381448832002E-4 1102 82-83 -0.04800907243264163 1102 84-85 -0.04793393451096151 1102 86-87 0.01082336994286237 1102 88-89 -0.03187025996104609 1102 90-91 0.012747290542598932 1102 92-93 -0.11927116514244673 1102 94-95 -0.24193526953063582 1102 96-97 -0.21925514052890804 1102 98-99 -0.1692129012553245 1102 100-101 -0.02307874273793331 1103 1 0.17901925794637208 1103 2 0.24750550400978355 1103 3 0.24862657697551782 1103 4 0.3158665107987204 1103 5 0.27124787770466696 1103 6 0.3993610989113989 1103 7 0.2798120794435093 1103 8 0.3435577093503568 1103 9 0.42906981022058943 1103 10-11 0.374194181019476 1103 12-13 0.4661257904165268 1103 14-15 0.6527046985083018 1103 16-17 0.6285000741907538 1103 18-19 0.5992549082008978 1103 20-21 0.5842476668810548 1103 22-23 0.6499964805119092 1103 24-25 0.5547991459786772 1103 26-27 0.6186655234691827 1103 28-29 0.5175530356149523 1103 30-31 0.5297328251898747 1103 32-33 0.5640025151831054 1103 34-35 0.5744663483519474 1103 36-37 0.6849231059048577 1103 38-39 0.5718223728226235 1103 40-41 0.6563220126085767 1103 42-43 0.567893598793205 1103 44-45 0.691888210765903 1103 46-47 0.5341954354860121 1103 48-49 0.6255253927981741 1103 50-51 0.6228707412374987 1103 52-53 0.666134503619304 1103 54-55 0.6444214663569028 1103 56-57 0.5917224234862246 1103 58-59 0.49283271468431167 1103 60-61 0.6143690362617846 1103 62-63 0.6001559032622126 1103 64-65 0.4428441565125425 1103 66-67 0.4524230796813242 1103 68-69 0.30556357421502156 1103 70-71 0.3105933637558316 1103 72-73 0.4146510453626 1103 74-75 0.44495957796014096 1103 76-77 0.437677804536424 1103 78-79 0.39866192598679007 1103 80-81 0.21480513756176123 1103 82-83 0.3379760413098225 1103 84-85 0.35681682943097925 1103 86-87 0.2585806167809963 1103 88-89 0.22256909153712812 1103 90-91 0.2206830027062523 1103 92-93 0.05771163555359138 1103 94-95 -0.1268280524013612 1103 96-97 -0.17125597874298393 1103 98-99 -0.007513183489432862 1103 100-101 0.1397997798832371 1104 1 0.24001279116212615 1104 2 0.2820734052443541 1104 3 0.20494271454165514 1104 4 0.392883706565911 1104 5 0.25392350968585475 1104 6 0.19937947045754356 1104 7 0.17564541277683787 1104 8 0.2489993613197825 1104 9 0.3135421794151796 1104 10-11 0.24241691764498796 1104 12-13 0.30845750705379515 1104 14-15 0.39632609768526095 1104 16-17 0.44759362210374576 1104 18-19 0.3744753667546945 1104 20-21 0.45348267569939793 1104 22-23 0.4805538732220711 1104 24-25 0.4159470001820864 1104 26-27 0.4565770461029288 1104 28-29 0.4332864277372295 1104 30-31 0.5374617346548973 1104 32-33 0.4597256192395349 1104 34-35 0.5463156281873367 1104 36-37 0.5676593639883407 1104 38-39 0.5933979166203898 1104 40-41 0.45404945234990635 1104 42-43 0.5462206858008471 1104 44-45 0.5145623730234021 1104 46-47 0.5384025195542108 1104 48-49 0.6861459836270924 1104 50-51 0.7850786336537254 1104 52-53 0.7709515230196544 1104 54-55 0.8265147203251644 1104 56-57 0.7849984375955756 1104 58-59 0.68348527200353 1104 60-61 0.7132153031636008 1104 62-63 0.6241675140793816 1104 64-65 0.6403162317623909 1104 66-67 0.5636389086054905 1104 68-69 0.6315795207170751 1104 70-71 0.5758197012043915 1104 72-73 0.4486328942750042 1104 74-75 0.321642411587419 1104 76-77 0.3303236746128526 1104 78-79 0.4157768583794983 1104 80-81 0.33937524338186975 1104 82-83 0.23169113537213804 1104 84-85 0.470797576050618 1104 86-87 0.22928718644589452 1104 88-89 0.14949442957357917 1104 90-91 0.16516235308838034 1104 92-93 0.1661570353184345 1104 94-95 0.12106840556454301 1104 96-97 0.2580631717567208 1104 98-99 0.2845300998732938 1104 100-101 0.45983395095907653 1105 1 0.09890733459941004 1105 2 0.15151987645434062 1105 3 0.07819721051948036 1105 4 0.1699616766187333 1105 5 0.2083490733433777 1105 6 0.20237729888661704 1105 7 0.18115897986889706 1105 8 0.2376499841614077 1105 9 0.22224238280556108 1105 10-11 0.22889813653924307 1105 12-13 0.13127906551667223 1105 14-15 0.21497290153973836 1105 16-17 0.09532277790363963 1105 18-19 0.14639693934119435 1105 20-21 0.1246193164081717 1105 22-23 0.24866984296182437 1105 24-25 0.19671929218113604 1105 26-27 0.2692349489844048 1105 28-29 0.1978430657640402 1105 30-31 0.13725138443490437 1105 32-33 0.2811121257228919 1105 34-35 0.19524095806573882 1105 36-37 0.17220821617620174 1105 38-39 0.20911845344047464 1105 40-41 0.2377796748148313 1105 42-43 0.18923066462561167 1105 44-45 0.2836950397420779 1105 46-47 0.3259666431017578 1105 48-49 0.16547565195647707 1105 50-51 0.34139862398644283 1105 52-53 0.33877704068616765 1105 54-55 0.3749047036674398 1105 56-57 0.3396641283555155 1105 58-59 0.3129975021951523 1105 60-61 0.44110622289705503 1105 62-63 0.37706680856101826 1105 64-65 0.2583440945623465 1105 66-67 0.2970779396738905 1105 68-69 0.34203171632876916 1105 70-71 0.2833228892173629 1105 72-73 0.3477862671442864 1105 74-75 0.25251492013170207 1105 76-77 0.1734866933564838 1105 78-79 0.18824654994912393 1105 80-81 0.1541762914873388 1105 82-83 0.12932405167615713 1105 84-85 0.04857667951151612 1105 86-87 0.10457953781514817 1105 88-89 0.16610562150037111 1105 90-91 0.16617548092043322 1105 92-93 -0.08559503338458185 1105 94-95 0.1035305101476851 1105 96-97 0.13271180199463473 1105 98-99 0.18303592752531372 1105 100-101 0.4296638250242779 1106 1 0.2711771001042145 1106 2 0.3702244517287312 1106 3 0.24827900787794732 1106 4 0.32827701695922684 1106 5 0.2726110978178866 1106 6 0.24005998336028256 1106 7 0.2639737511051763 1106 8 0.29268150222414846 1106 9 0.3437676124183895 1106 10-11 0.3097305196808122 1106 12-13 0.21064585368658584 1106 14-15 0.353716187019792 1106 16-17 0.24761741518309321 1106 18-19 0.37423409568008736 1106 20-21 0.3420794184628093 1106 22-23 0.27673120412163144 1106 24-25 0.23208019825973025 1106 26-27 0.06998087478454096 1106 28-29 0.31192116748307797 1106 30-31 0.19157202765407533 1106 32-33 0.2624915261721128 1106 34-35 0.38924636000696466 1106 36-37 0.48265974426649905 1106 38-39 0.42995590595616306 1106 40-41 0.43106497797654697 1106 42-43 0.5162046627292725 1106 44-45 0.46779355542125245 1106 46-47 0.5141009466415198 1106 48-49 0.6069304460782234 1106 50-51 0.6440776593194215 1106 52-53 0.5187214999562997 1106 54-55 0.523220792031232 1106 56-57 0.5552089369727398 1106 58-59 0.42001802936962207 1106 60-61 0.4892358361285787 1106 62-63 0.4582873551436677 1106 64-65 0.3557895444579273 1106 66-67 0.2636857753190185 1106 68-69 0.4474539754804212 1106 70-71 0.26351960418206843 1106 72-73 0.2507847033712558 1106 74-75 0.22824712374768907 1106 76-77 0.05509684383046576 1106 78-79 0.26701024433510767 1106 80-81 0.10778507429169792 1106 82-83 0.20868970264848485 1106 84-85 0.2729902393543888 1106 86-87 0.3814213177466925 1106 88-89 0.35767565164368875 1106 90-91 0.3758580360062851 1106 92-93 0.3249933122102675 1106 94-95 0.34857675112844433 1106 96-97 0.4714357045737003 1106 98-99 0.5994579703567204 1106 100-101 0.7643658388242933 1107 1 -0.025695151668298166 1107 2 0.015353354338120795 1107 3 0.08484653113237428 1107 4 0.15319382463839304 1107 5 -0.04172320188067857 1107 6 0.07286009738329113 1107 7 -0.1886010663872426 1107 8 -0.04306698606119852 1107 9 -0.12178768368764281 1107 10-11 -0.1073262057562232 1107 12-13 0.05871474056726811 1107 14-15 0.06001482116968759 1107 16-17 -0.008175342940539565 1107 18-19 0.05171639583151233 1107 20-21 0.06188442192999588 1107 22-23 0.09718097334633313 1107 24-25 -0.022274636700053918 1107 26-27 0.1428978573394204 1107 28-29 0.01686590473484273 1107 30-31 0.13667318264248252 1107 32-33 0.18585338042078092 1107 34-35 0.18801343566042306 1107 36-37 0.34393414852659276 1107 38-39 0.4268114282887012 1107 40-41 0.38579933367577013 1107 42-43 0.34216503872217174 1107 44-45 0.4490607523757859 1107 46-47 0.5318064812052512 1107 48-49 0.3872160393516779 1107 50-51 0.4797334028242517 1107 52-53 0.44820172990270635 1107 54-55 0.4984953367984062 1107 56-57 0.3601902919866191 1107 58-59 0.32875350103874723 1107 60-61 0.21560752385109083 1107 62-63 0.2075091329322234 1107 64-65 0.1778294949670851 1107 66-67 0.24952084224688775 1107 68-69 0.21439367065082848 1107 70-71 0.26626593634080464 1107 72-73 0.19861020469849322 1107 74-75 0.2168109594494254 1107 76-77 0.18201516979475585 1107 78-79 0.16598578094074057 1107 80-81 0.06759057307161243 1107 82-83 0.1225301806151542 1107 84-85 0.03628989142247363 1107 86-87 0.1376815904315123 1107 88-89 0.15960085133558266 1107 90-91 0.26736079459490725 1107 92-93 0.3496463658637552 1107 94-95 0.3236808426137152 1107 96-97 0.19038743078908738 1107 98-99 0.1763866069124198 1107 100-101 0.3411989507831663 1108 1 0.11705122597834006 1108 2 0.13988645639073738 1108 3 0.16140255100149048 1108 4 0.11507188875409469 1108 5 0.19807729828409038 1108 6 0.27108562438592543 1108 7 0.3109434480748732 1108 8 0.25812439266703535 1108 9 0.2085811322319131 1108 10-11 0.25479462224491556 1108 12-13 0.2708556438963754 1108 14-15 0.16718355298715437 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-3.9452486711711643 2316 94-95 -3.90000112819833 2316 96-97 -3.9999580639794416 2316 98-99 -4.512332108723079 2316 100-101 -4.851054033973835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12949.0 3 4223.0 4 4250.0 5 4654.0 6 5355.0 7 6432.0 8 7915.0 9 9652.0 10 11917.0 11 14969.0 12 18993.0 13 23481.0 14 24084.0 15 23747.0 16 28782.0 17 34950.0 18 30166.0 19 23392.0 20 24301.0 21 26052.0 22 28810.0 23 32843.0 24 37656.0 25 43147.0 26 49911.0 27 57639.0 28 67367.0 29 78893.0 30 93387.0 31 111329.0 32 135308.0 33 168668.0 34 219459.0 35 300419.0 36 434948.0 37 640497.0 38 590377.0 39 96745.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73939302396628 19.599212518838108 14.617406127452929 23.04398832974269 2 14.965174372151303 21.15332015289909 40.314650710926294 23.566854764023315 3 18.388946420211933 25.641751663285717 30.946874157717787 25.02242775878456 4 12.5438550691409 17.32898083713666 38.384408196343585 31.742755897378856 5 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22.192874912436327 50-51 21.83738679728883 27.5077746767671 28.379079569402517 22.27575895654155 52-53 22.066863249848375 27.55500581504387 28.196162996669326 22.18196793843843 54-55 21.758871440733678 27.31104994930158 28.379938717632502 22.550139892332243 56-57 21.933232306048613 27.624423357944792 28.193146705710937 22.249197630295658 58-59 22.073132615312094 27.233284030574545 28.261739009640795 22.43184434447257 60-61 21.983824182678998 27.24947360722548 28.56110865902241 22.205593551073118 62-63 21.876215024554973 27.22782560166628 28.52628154614833 22.369677827630415 64-65 21.701712331352187 27.22378831574439 28.486449141000115 22.5880502119033 66-67 21.70693131071297 27.38484786551692 28.15433992925766 22.75388089451245 68-69 21.653177842100487 27.16916437468939 28.424402069196464 22.753255714013655 70-71 21.620400143289842 27.170687969279573 28.368568248196237 22.840343639234355 72-73 21.73458100136169 26.95493603216102 28.37808141060945 22.932401555867838 74-75 21.50854692395188 27.291055689762988 28.18823668807925 23.01216069820588 76-77 21.577127368742396 26.999648020485434 28.339006962132018 23.084217648640152 78-79 21.551411338681 27.060068495402916 28.28697293393862 23.101547231977467 80-81 21.501635743817506 27.080831126693738 28.235524902155028 23.18200822733373 82-83 21.561625155347993 26.962645436972604 28.27836765760498 23.19736175007443 84-85 21.500863973658095 26.789746667860676 28.222460866342697 23.48692849213853 86-87 21.50191836231404 26.703096518413744 28.39609077258187 23.398894346690344 88-89 21.511298475617526 26.518205738019024 28.315489512794844 23.655006273568606 90-91 21.458684786382527 26.567102029353872 28.434451650646153 23.539761533617447 92-93 21.60756673557497 26.433559502196847 28.415817275457954 23.543056486770233 94-95 21.41824210987148 26.230549009901132 28.460703239223072 23.890505641004314 96-97 21.475406263890406 26.06922212871554 28.49023489767048 23.965136709723573 98-99 21.405804077494032 25.998197084574677 28.32039772371247 24.275601114218823 100-101 21.566878545553962 25.56380003645228 28.787847720593263 24.081473697400497 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2203.0 1 1322.0 2 589.5 3 737.5 4 687.0 5 749.5 6 1163.0 7 2143.0 8 3424.0 9 4153.5 10 4021.5 11 3850.5 12 4238.5 13 4748.5 14 5074.5 15 5431.0 16 5846.0 17 6168.5 18 6514.5 19 6938.5 20 7502.5 21 8459.0 22 9658.5 23 11345.0 24 13474.5 25 16459.0 26 21409.0 27 27028.0 28 32075.5 29 37799.5 30 44457.0 31 50976.0 32 57803.5 33 64795.5 34 72979.0 35 80199.5 36 87441.5 37 95778.0 38 100330.0 39 101810.5 40 104686.5 41 110700.0 42 116622.0 43 122684.0 44 128285.0 45 131658.0 46 133531.0 47 137320.5 48 147092.5 49 148729.5 50 139226.5 51 130399.5 52 121831.5 53 115334.0 54 109159.5 55 99548.5 56 88486.0 57 77734.0 58 68624.5 59 59715.5 60 50355.0 61 41182.5 62 33123.5 63 25850.0 64 19003.5 65 14159.0 66 10644.5 67 8239.0 68 6205.0 69 4270.5 70 3084.5 71 2293.0 72 1774.0 73 1431.0 74 1125.5 75 908.5 76 649.0 77 399.0 78 250.0 79 179.5 80 124.0 81 89.0 82 70.0 83 48.0 84 36.5 85 28.5 86 23.0 87 19.0 88 14.5 89 10.0 90 9.5 91 10.5 92 7.0 93 4.0 94 5.0 95 4.0 96 3.0 97 3.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15803538348455123 2 0.09283692931155252 3 0.0862036952782996 4 0.1015678527399367 5 0.1219211093035076 6 0.03855212942403402 7 0.04223725944250786 8 0.046064125230923 9 0.04629090246282908 10-11 0.04461842037752173 12-13 0.04834607112697796 14-15 0.04644681180976452 16-17 0.045298752073239974 18-19 0.04956499874847315 20-21 0.05157764668163963 22-23 0.06436221313034504 24-25 0.08088860390550079 26-27 0.08251856525982576 28-29 0.0731356572897116 30-31 0.061428282692560086 32-33 0.050500454830085736 34-35 0.29172056169318683 36-37 0.46465237459856884 38-39 0.44125179898125994 40-41 0.41666064289644417 42-43 0.44676532043197664 44-45 0.45664430359688535 46-47 0.4564742206729558 48-49 0.47267461917724657 50-51 0.46180348562274876 52-53 0.4688477533888314 54-55 0.4603719543463416 56-57 0.4376375368477568 58-59 0.41678820508939135 60-61 0.38778906655940104 62-63 0.444299118034998 64-65 0.4821709157633137 66-67 0.4837016620786798 68-69 0.4943601919782657 70-71 0.49107192211562745 72-73 0.4813771954516425 74-75 0.47480065572636604 76-77 0.45704116375272097 78-79 0.43066413696664474 80-81 0.43582331899250815 82-83 0.44838110820930743 84-85 0.44563143427244617 86-87 0.45644587351896754 88-89 0.46862097615692533 90-91 0.4726037512922759 92-93 0.47467309353341886 94-95 0.4784999593218341 96-97 0.4804700865240181 98-99 0.473709290297818 100-101 0.4606695994632183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3527691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.99838528236708 #Duplication Level Percentage of deduplicated Percentage of total 1 76.84015876418509 43.79764974399278 2 13.215883479910254 15.065680368695899 3 4.3925763577305075 7.511092788604206 4 1.998240214472163 4.555858625248168 5 0.9991114651887697 2.8473870116429896 6 0.6009803337744409 2.055294516696069 7 0.36836706324208013 1.4697429457204314 8 0.2658767468554648 1.2123636203912131 9 0.1936131758273977 0.9932074552397425 >10 0.9944990248102513 10.359295400956967 >50 0.08191187890348002 3.1944088350814805 >100 0.04549201586074768 4.660295286356139 >500 0.0025917109212995155 0.988922518312094 >1k 5.482465356070061E-4 0.4980549095562859 >5k 9.96811882921829E-5 0.44001668344269396 >10k+ 4.984059414609145E-5 0.35072929006285103 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12346 0.34997396313906176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9122 0.25858273868091053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6367 0.1804863294432534 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.669430797652062E-5 0.0 5 0.0 0.0 0.0 5.669430797652062E-5 0.0 6 0.0 0.0 0.0 5.669430797652062E-5 0.0 7 0.0 0.0 0.0 5.669430797652062E-5 0.0 8 0.0 0.0 0.0 5.669430797652062E-5 2.834715398826031E-5 9 0.0 0.0 0.0 8.504146196478092E-5 2.834715398826031E-5 10-11 0.0 0.0 0.0 9.921503895891108E-5 2.834715398826031E-5 12-13 0.0 0.0 0.0 1.2756219294717138E-4 2.834715398826031E-5 14-15 0.0 0.0 0.0 1.7008292392956185E-4 2.834715398826031E-5 16-17 0.0 0.0 0.0 2.2677723190608247E-4 5.669430797652062E-5 18-19 0.0 0.0 0.0 2.6929796288847294E-4 5.669430797652062E-5 20-21 0.0 0.0 0.0 3.8268657884151414E-4 5.669430797652062E-5 22-23 0.0 0.0 0.0 7.228524267006379E-4 5.669430797652062E-5 24-25 0.0 0.0 0.0 0.0013606633914364948 8.504146196478092E-5 26-27 0.0 0.0 0.0 0.0024378552429903865 8.504146196478092E-5 28-29 0.0 1.4173576994130155E-5 0.0 0.0067891433801883444 8.504146196478092E-5 30-31 0.0 2.834715398826031E-5 0.0 0.0227485910755789 8.504146196478092E-5 32-33 0.0 2.834715398826031E-5 0.0 0.056141538473749544 1.4173576994130154E-4 34-35 0.0 2.834715398826031E-5 0.0 0.10576323153019922 1.7008292392956185E-4 36-37 0.0 2.834715398826031E-5 0.0 0.183165135495144 1.7008292392956185E-4 38-39 0.0 2.834715398826031E-5 0.0 0.30155702412711316 2.1260365491195232E-4 40-41 0.0 2.834715398826031E-5 0.0 0.43931001893306415 2.551243858943428E-4 42-43 0.0 2.834715398826031E-5 0.0 0.5791890502881346 2.551243858943428E-4 44-45 0.0 2.834715398826031E-5 0.0 0.7474294092084596 2.551243858943428E-4 46-47 0.0 2.834715398826031E-5 0.0 0.9432232018053734 2.551243858943428E-4 48-49 0.0 2.834715398826031E-5 0.0 1.155174872175596 2.8347153988260307E-4 50-51 0.0 2.834715398826031E-5 0.0 1.3833694617810914 2.8347153988260307E-4 52-53 0.0 2.834715398826031E-5 0.0 1.61689331633638 2.9764511687673325E-4 54-55 0.0 2.834715398826031E-5 0.0 1.8709405103791688 3.118186938708634E-4 56-57 0.0 2.834715398826031E-5 0.0 2.165084753738352 3.259922708649935E-4 58-59 0.0 2.834715398826031E-5 0.0 2.487349373853889 3.968601558356443E-4 60-61 0.0 2.834715398826031E-5 0.0 2.841050987742407 4.2520730982390463E-4 62-63 0.0 2.834715398826031E-5 0.0 3.200181081619677 4.2520730982390463E-4 64-65 0.0 2.834715398826031E-5 0.0 3.5705791692072806 4.2520730982390463E-4 66-67 0.0 2.834715398826031E-5 0.0 3.996665240804821 4.5355446381216495E-4 68-69 0.0 2.834715398826031E-5 0.0 4.462564890178873 4.8190161780042526E-4 70-71 0.0 2.834715398826031E-5 0.0 4.968986796179144 5.102487717886856E-4 72-73 0.0 2.834715398826031E-5 0.0 5.4852593381903345 6.236373877417268E-4 74-75 0.0 2.834715398826031E-5 0.0 6.025300968820682 6.236373877417268E-4 76-77 0.0 2.834715398826031E-5 0.0 6.598012694422499 6.236373877417268E-4 78-79 0.0 2.834715398826031E-5 0.0 7.230466047054575 6.236373877417268E-4 80-81 0.0 2.834715398826031E-5 0.0 7.900153386450231 6.236373877417268E-4 82-83 0.0 2.834715398826031E-5 0.0 8.592589316921465 6.519845417299871E-4 84-85 0.0 2.834715398826031E-5 0.0 9.302331184902533 6.519845417299871E-4 86-87 0.0 2.834715398826031E-5 0.0 10.038393385361701 6.519845417299871E-4 88-89 0.0 2.834715398826031E-5 0.0 10.811788787623406 6.519845417299871E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18275 0.0 30.275612 1 GGTATCA 12460 0.0 28.385742 1 GTACATG 14510 0.0 25.38827 1 ATCAACG 22330 0.0 24.678442 3 TATCAAC 22375 0.0 24.671164 2 TCAACGC 22395 0.0 24.585655 4 TACATGG 14725 0.0 24.552511 2 CAACGCA 22685 0.0 24.292244 5 AACGCAG 23120 0.0 23.859924 6 ACATGGG 15160 0.0 23.31666 3 GAGTACT 11950 0.0 21.538322 12-13 ACGCAGA 25540 0.0 21.43317 7 CGCAGAG 25825 0.0 21.159966 8 CATGGGG 5960 0.0 20.591131 4 GCAGAGT 26665 0.0 20.103552 9 AGTACTT 12685 0.0 20.085009 12-13 AGAGTAC 20895 0.0 19.501879 10-11 CATGGGA 8930 0.0 19.261135 4 GTACTTT 13795 0.0 18.5728 14-15 CAGAGTA 26095 0.0 16.532139 10-11 >>END_MODULE