FastQCFastQC Report
Thu 26 May 2016
SRR938749_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938749_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527691
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125890.356862321558209No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100910.2860511308955348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71090.20151991770254252No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38800.10998695747445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA195650.029.676651
GGTATCA134400.028.3873121
ATCAACG238400.023.9464323
TCAACGC239500.023.8364494
TATCAAC242350.023.7519472
CAACGCA243200.023.512835
AACGCAG249450.022.904696
GTACATG148850.022.4635981
AGTACTT134150.021.22562412-13
TACATGG154800.021.0297742
GAGTACT129550.020.99022712-13
ACGCAGA277750.020.519677
CGCAGAG277850.020.3585728
ACATGGG153750.020.2165783
AGAGTAC228050.019.74844710-11
GCAGAGT291200.019.1644959
GTACTTT142550.018.82658214-15
CATGGGG61550.018.5810594
CATGGGA94400.016.9410134
TACTTTT166550.016.89725514-15
Produced by FastQC (version 0.11.5)