##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938749_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3527691 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.978428949701094 31.0 31.0 34.0 30.0 34.0 2 32.197039083071616 34.0 31.0 34.0 30.0 34.0 3 32.28651885893634 34.0 31.0 34.0 30.0 34.0 4 35.92978580040032 37.0 35.0 37.0 35.0 37.0 5 35.8356227912252 37.0 35.0 37.0 35.0 37.0 6 35.85977144823625 37.0 35.0 37.0 35.0 37.0 7 35.79002100807582 37.0 35.0 37.0 33.0 37.0 8 35.75681883702399 37.0 35.0 37.0 33.0 37.0 9 37.39960586117095 39.0 37.0 39.0 34.0 39.0 10-11 37.32896319433873 39.0 37.0 39.0 34.0 39.0 12-13 37.261703476863474 39.0 37.0 39.0 33.0 39.0 14-15 38.47805023172381 40.0 38.0 41.0 33.5 41.0 16-17 38.420626976682485 40.0 38.0 41.0 33.0 41.0 18-19 38.445587779655305 40.0 38.0 41.0 33.5 41.0 20-21 38.51344618902279 40.0 38.0 41.0 34.0 41.0 22-23 38.464207891224035 40.0 38.0 41.0 34.0 41.0 24-25 38.397874133533804 40.0 38.0 41.0 34.0 41.0 26-27 38.24564594801529 40.0 38.0 41.0 33.0 41.0 28-29 38.086586381857146 40.0 37.5 41.0 33.0 41.0 30-31 37.862269257709926 40.0 37.0 41.0 33.0 41.0 32-33 37.64509320685967 40.0 37.0 41.0 32.0 41.0 34-35 37.60688776312891 40.0 37.0 41.0 32.0 41.0 36-37 37.519666405022434 40.0 37.0 41.0 32.0 41.0 38-39 37.42869868704487 40.0 37.0 41.0 32.0 41.0 40-41 37.279424983650784 40.0 36.0 41.0 31.0 41.0 42-43 37.1058136894643 40.0 36.0 41.0 31.0 41.0 44-45 36.855681379123055 40.0 36.0 41.0 30.5 41.0 46-47 36.556344220624766 39.5 36.0 41.0 30.0 41.0 48-49 36.28311720045775 39.0 35.0 41.0 30.0 41.0 50-51 36.05842830338598 39.0 35.0 41.0 29.0 41.0 52-53 35.86880908787079 39.0 35.0 41.0 28.5 41.0 54-55 35.63820187198935 39.0 35.0 40.0 28.0 41.0 56-57 35.39477040364363 38.5 35.0 40.0 28.0 41.0 58-59 35.10893060078108 38.0 34.0 40.0 27.0 41.0 60-61 34.78579161270078 38.0 34.0 40.0 26.0 41.0 62-63 34.43526643915241 37.5 34.0 40.0 26.0 41.0 64-65 34.08947580726317 37.0 33.5 40.0 26.0 41.0 66-67 33.70880924661485 36.0 33.0 39.0 25.0 41.0 68-69 33.272571633966805 36.0 32.5 39.0 24.0 40.0 70-71 32.81367316468477 35.0 32.0 38.5 23.0 40.0 72-73 32.475691181568905 35.0 32.0 38.0 22.0 40.0 74-75 32.0542665159732 35.0 31.5 37.0 21.5 39.0 76-77 29.97703923047682 33.0 29.0 35.5 18.5 37.5 78-79 31.03831684804593 34.0 31.0 36.0 20.0 38.5 80-81 31.085786141700055 35.0 31.0 36.0 20.0 37.5 82-83 30.77729313593509 34.0 31.0 35.0 18.0 37.0 84-85 30.391425864680325 34.0 31.0 35.0 16.5 36.5 86-87 29.86217060961405 34.0 30.0 35.0 8.5 36.0 88-89 29.359773432537033 34.0 29.5 35.0 3.5 36.0 90-91 28.96226129215966 34.0 29.0 35.0 2.0 35.0 92-93 28.6852796914469 34.0 29.0 35.0 2.0 35.0 94-95 28.310508488413525 34.0 29.0 35.0 2.0 35.0 96-97 27.80956339429956 34.0 28.5 35.0 2.0 35.0 98-99 27.106706483079158 33.0 27.0 35.0 2.0 35.0 100-101 24.096897659120373 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03261382394307688 1101 2 0.08550177617504318 1101 3 0.09173764674617502 1101 4 0.04404099435957676 1101 5 0.03946237951296894 1101 6 0.1275258900826799 1101 7 0.10195533907456422 1101 8 0.0874264353624028 1101 9 0.19529774232680808 1101 10-11 0.15535818319347783 1101 12-13 0.16351450728281236 1101 14-15 0.2314536113233956 1101 16-17 0.1310273200077674 1101 18-19 0.19392069583880556 1101 20-21 0.18029625479455547 1101 22-23 0.16019325431530973 1101 24-25 0.1839419292585447 1101 26-27 0.2353191357265132 1101 28-29 0.28044711835431 1101 30-31 0.27827398897731825 1101 32-33 0.3437747724430835 1101 34-35 0.2748550637345417 1101 36-37 0.36304373722072114 1101 38-39 0.3900182512409316 1101 40-41 0.4889492143464551 1101 42-43 0.3244678817063118 1101 44-45 0.43242603035366045 1101 46-47 0.4630214299216391 1101 48-49 0.38215110748822667 1101 50-51 0.504095445693963 1101 52-53 0.5748605613938693 1101 54-55 0.45524067649338207 1101 56-57 0.38137876128462267 1101 58-59 0.4778395286744299 1101 60-61 0.43788224898484174 1101 62-63 0.4344598563426416 1101 64-65 0.37687334410951934 1101 66-67 0.2882819476575307 1101 68-69 0.25552491100205543 1101 70-71 0.10248789544717596 1101 72-73 0.24183319935978886 1101 74-75 0.2065631912816812 1101 76-77 -0.013060578916110899 1101 78-79 0.1296165659930253 1101 80-81 0.11470318042179883 1101 82-83 0.13889658880735567 1101 84-85 0.1468185663478323 1101 86-87 0.19736594831348597 1101 88-89 0.28533967575028285 1101 90-91 0.3844830820005285 1101 92-93 0.33463349009166876 1101 94-95 0.527643461366722 1101 96-97 0.5447217413878533 1101 98-99 0.5223448683325209 1101 100-101 1.1614418720325048 1102 1 0.11244705153303514 1102 2 0.09561889955239167 1102 3 0.14319438763461534 1102 4 0.11805330087437937 1102 5 0.108140184481897 1102 6 0.1750288357910108 1102 7 0.14953582992524872 1102 8 0.14694484981600908 1102 9 0.18710716255098703 1102 10-11 0.24942547626375955 1102 12-13 0.29609555202736715 1102 14-15 0.2802682784331836 1102 16-17 0.3236998075955029 1102 18-19 0.3351601089127101 1102 20-21 0.3159609777972676 1102 22-23 0.2969471340901251 1102 24-25 0.24492614831854098 1102 26-27 0.2951955670386397 1102 28-29 0.2824718522334848 1102 30-31 0.24865577291842555 1102 32-33 0.31701062045461015 1102 34-35 0.3093782618170593 1102 36-37 0.27043721335796533 1102 38-39 0.29332852244751706 1102 40-41 0.3157868831581965 1102 42-43 0.4120823111452623 1102 44-45 0.24461270265998536 1102 46-47 0.21616502942615767 1102 48-49 0.27549431020331383 1102 50-51 0.23953256866847283 1102 52-53 0.2955988515738355 1102 54-55 0.25743359269427657 1102 56-57 0.186200155687267 1102 58-59 0.2717915111109548 1102 60-61 0.15381878281768735 1102 62-63 0.09382771237052623 1102 64-65 0.16911329093570515 1102 66-67 0.21736389373848652 1102 68-69 0.26713049828062907 1102 70-71 0.1347104197933291 1102 72-73 0.2513969324030825 1102 74-75 0.36276126373100226 1102 76-77 0.24903092435186025 1102 78-79 0.10068819957712094 1102 80-81 0.23978953751214505 1102 82-83 0.3832503960724303 1102 84-85 0.423903976783194 1102 86-87 0.38127130295663036 1102 88-89 0.5352908323813992 1102 90-91 0.6241630320493741 1102 92-93 0.5661973842677241 1102 94-95 0.5068185623264725 1102 96-97 0.5934724589321938 1102 98-99 0.6310102862202704 1102 100-101 0.8756870153903122 1103 1 0.03466184654194748 1103 2 0.056584118973489694 1103 3 0.032667373798780375 1103 4 0.08065409876929408 1103 5 0.027696567641221748 1103 6 0.026886169633641543 1103 7 0.03009616011792815 1103 8 0.11137857785268324 1103 9 0.021809225194225235 1103 10-11 0.048549860866089034 1103 12-13 0.14028004400597638 1103 14-15 0.10458099594443127 1103 16-17 0.08473425925495093 1103 18-19 0.17913902623721611 1103 20-21 0.16716524738389182 1103 22-23 0.06827092875951024 1103 24-25 0.1077081171666805 1103 26-27 0.07862041425025268 1103 28-29 0.1931346779698444 1103 30-31 0.17375948864713564 1103 32-33 0.09244007253846576 1103 34-35 0.048411450850245785 1103 36-37 0.031995283555744436 1103 38-39 0.22187793463663041 1103 40-41 0.2454564782689772 1103 42-43 0.24474082909789985 1103 44-45 0.28530958747450796 1103 46-47 0.3034382341625985 1103 48-49 0.30719100403236155 1103 50-51 0.33996679538946495 1103 52-53 0.27228058487321505 1103 54-55 0.1611607274875908 1103 56-57 0.15946271828806857 1103 58-59 0.03383341120431993 1103 60-61 0.059433490822101476 1103 62-63 0.09185286940302717 1103 64-65 0.2125043973782894 1103 66-67 0.09898843749537889 1103 68-69 0.09195595876784779 1103 70-71 -0.1074626236664713 1103 72-73 0.018364401208785353 1103 74-75 0.024869743486540585 1103 76-77 0.02238314402761077 1103 78-79 0.007663148647658602 1103 80-81 0.1610952946634896 1103 82-83 0.04131422766567283 1103 84-85 0.06186552503885778 1103 86-87 0.19573500510415442 1103 88-89 0.4220688011446647 1103 90-91 0.4725948290105819 1103 92-93 0.4332107426413785 1103 94-95 0.3276776256487679 1103 96-97 0.3018336443154368 1103 98-99 0.4053198206400985 1103 100-101 0.9968736718710858 1104 1 -0.03359947033048272 1104 2 -0.012863999780179824 1104 3 0.03744030148837396 1104 4 -0.0336316155164198 1104 5 -0.02685590149457795 1104 6 -0.014695987920738673 1104 7 -0.04567177051111315 1104 8 -0.010310869531210187 1104 9 -0.07409024570524281 1104 10-11 -0.04373960121504439 1104 12-13 0.019270343829603576 1104 14-15 0.09871496125894907 1104 16-17 0.1028136978205012 1104 18-19 0.07366246833010592 1104 20-21 0.04177577060552551 1104 22-23 -0.04193813943566482 1104 24-25 -0.05480474291562132 1104 26-27 -0.010167431134817662 1104 28-29 0.02822065680582142 1104 30-31 0.10062787489052027 1104 32-33 0.025371069011129066 1104 34-35 0.0935025737191566 1104 36-37 0.028076632762093823 1104 38-39 0.14163653252023067 1104 40-41 0.24622808320724232 1104 42-43 0.2460341859468187 1104 44-45 0.1708475063457584 1104 46-47 0.18943727884219896 1104 48-49 0.20009591290948947 1104 50-51 0.18008106640651533 1104 52-53 0.23522150198079572 1104 54-55 0.28781600379564765 1104 56-57 0.16636674533098272 1104 58-59 0.11136684741831715 1104 60-61 0.2926510834146896 1104 62-63 0.1202277224306556 1104 64-65 0.31527298938299 1104 66-67 0.15150533931783627 1104 68-69 0.20981677593422177 1104 70-71 0.2398569677503346 1104 72-73 0.20537571808121413 1104 74-75 0.16666868528548662 1104 76-77 0.040541580241601594 1104 78-79 0.1551811527628857 1104 80-81 0.23713696133015816 1104 82-83 0.19701675559042542 1104 84-85 0.3212276649565524 1104 86-87 0.3819802285021545 1104 88-89 0.6158481956185007 1104 90-91 0.4428329242486768 1104 92-93 0.3948307455808262 1104 94-95 0.4571363998992126 1104 96-97 0.5605325714171414 1104 98-99 0.5139967018864233 1104 100-101 1.0583375166682636 1105 1 -0.06649094328170335 1105 2 -0.03955560968466898 1105 3 -0.005042998728370662 1105 4 -0.04883006263160894 1105 5 -0.0570799470409753 1105 6 -0.0787236783818912 1105 7 -0.04245087040286677 1105 8 -0.09653839856494528 1105 9 0.0033016053206011975 1105 10-11 0.015644629172371083 1105 12-13 -0.04175594905559876 1105 14-15 -0.02493736712499839 1105 16-17 -0.03837716332795793 1105 18-19 -0.13185984317177457 1105 20-21 -0.06354707657430936 1105 22-23 -0.11084217680361519 1105 24-25 -0.05001023144770045 1105 26-27 -0.02072407528017095 1105 28-29 -0.051749010544590135 1105 30-31 -0.23418652692301123 1105 32-33 -0.10352012962565738 1105 34-35 0.0027582893254489704 1105 36-37 -0.0770459597846127 1105 38-39 -0.05593524939673955 1105 40-41 -0.09117828404879447 1105 42-43 -0.24897742142866974 1105 44-45 -0.1841221227637888 1105 46-47 -0.002088368282372244 1105 48-49 -0.125131250541628 1105 50-51 0.01002562225932735 1105 52-53 -0.13395728321417977 1105 54-55 -0.11351442119286759 1105 56-57 -0.29465103258956304 1105 58-59 -0.24747479616361545 1105 60-61 -0.3254856383647038 1105 62-63 -0.3109569589949359 1105 64-65 -0.2691083984338789 1105 66-67 -0.2457052704301148 1105 68-69 -0.17950436213414633 1105 70-71 -0.2721637914275945 1105 72-73 -0.31767383238473457 1105 74-75 -0.2380220914781468 1105 76-77 -0.26663360317430573 1105 78-79 -0.36548854156008304 1105 80-81 -0.2436894542317276 1105 82-83 -0.19812119958415053 1105 84-85 -0.1287954835102667 1105 86-87 -0.07009313990059596 1105 88-89 0.046306555656702386 1105 90-91 0.07333100379272395 1105 92-93 0.14734635677428187 1105 94-95 0.14895790380513318 1105 96-97 0.15614937863171363 1105 98-99 0.31978144765864513 1105 100-101 0.7801677700827909 1106 1 0.021520820900921223 1106 2 -0.07870351006413756 1106 3 -0.1021207547393459 1106 4 0.007410675525868271 1106 5 0.030400114094767616 1106 6 -0.0349499109524416 1106 7 -0.08691807564630949 1106 8 -0.06764448242037702 1106 9 -0.014404561019560447 1106 10-11 -0.027129667938439184 1106 12-13 -0.0046208883949532265 1106 14-15 0.011737381225813692 1106 16-17 -0.08665372775803348 1106 18-19 -0.09635527113207587 1106 20-21 -0.10391367369503257 1106 22-23 -0.06789186677828951 1106 24-25 -0.04016172132815399 1106 26-27 -0.04699043323559948 1106 28-29 0.03200622309137913 1106 30-31 0.12166470842736032 1106 32-33 0.1264883159617085 1106 34-35 0.10259893003772902 1106 36-37 0.01528386996933051 1106 38-39 -0.09410920322550709 1106 40-41 0.008505928818422603 1106 42-43 0.1237493206064002 1106 44-45 0.17769636736129257 1106 46-47 0.14940580825516747 1106 48-49 0.2392152298065895 1106 50-51 0.18648798253565957 1106 52-53 0.14811724820987848 1106 54-55 0.17169498489685253 1106 56-57 0.09054621812156682 1106 58-59 0.1684789739748851 1106 60-61 0.09888882715244307 1106 62-63 -4.350510098873883E-4 1106 64-65 0.2112941492930318 1106 66-67 0.24421612647307 1106 68-69 0.10598567904757061 1106 70-71 0.09602035169150014 1106 72-73 0.3077437718381617 1106 74-75 0.34454173815853295 1106 76-77 0.33408394322840707 1106 78-79 0.2948041326636428 1106 80-81 0.19143266562586092 1106 82-83 0.3124347737862223 1106 84-85 0.16194045011378222 1106 86-87 0.3918836481277985 1106 88-89 0.5129092107350708 1106 90-91 0.5811737500895049 1106 92-93 0.5454183267239614 1106 94-95 0.4867279503240951 1106 96-97 0.48434384245063455 1106 98-99 0.7051710111162919 1106 100-101 1.1489798989773128 1107 1 -0.1613160372394944 1107 2 -0.17283112283875823 1107 3 -0.21811035923719402 1107 4 -0.13324752432573916 1107 5 -0.09871047609561145 1107 6 -0.1379893621055075 1107 7 -0.11067868190907859 1107 8 -0.04825854889741521 1107 9 -0.19820171933970698 1107 10-11 -0.10678960037373741 1107 12-13 -0.16823759114869574 1107 14-15 -0.1848101627306704 1107 16-17 -0.15979953973732108 1107 18-19 -0.18866816927758379 1107 20-21 -0.21836060649626177 1107 22-23 -0.21101563734134032 1107 24-25 -0.21103943063821617 1107 26-27 -0.25648585614169406 1107 28-29 -0.23532807963970725 1107 30-31 -0.2654094237139901 1107 32-33 -0.2609355676135152 1107 34-35 -0.28027148806686597 1107 36-37 -0.265539744115344 1107 38-39 -0.2872159663669649 1107 40-41 -0.1471193074659567 1107 42-43 -0.3280465165428623 1107 44-45 -0.23457022915292214 1107 46-47 -0.25996636770416615 1107 48-49 -0.43331280590444976 1107 50-51 -0.4440073352428371 1107 52-53 -0.3940035393575698 1107 54-55 -0.5496231231298481 1107 56-57 -0.3734310268075376 1107 58-59 -0.4284519571928129 1107 60-61 -0.4299956123456141 1107 62-63 -0.47950278148698544 1107 64-65 -0.4046967187576769 1107 66-67 -0.42841703683659205 1107 68-69 -0.3244884615848562 1107 70-71 -0.410354019817305 1107 72-73 -0.42565301712021153 1107 74-75 -0.3553970553405321 1107 76-77 -0.47276254217401714 1107 78-79 -0.3417822680706344 1107 80-81 -0.4451927788920358 1107 82-83 -0.3154961634465394 1107 84-85 -0.3853725288991079 1107 86-87 -0.4157132613229706 1107 88-89 -0.11746910204783845 1107 90-91 0.1272483209364097 1107 92-93 0.0038077618046372663 1107 94-95 -0.10602908665453725 1107 96-97 -0.06949387086235959 1107 98-99 0.013045396776885099 1107 100-101 0.3326395827454114 1108 1 -0.17408523970513912 1108 2 -0.12765455901518408 1108 3 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-1.078851643816023 2316 52-53 -1.1012873887992853 2316 54-55 -1.1479697944949336 2316 56-57 -1.0957127495872285 2316 58-59 -0.9011281896435932 2316 60-61 -0.8816703640253039 2316 62-63 -0.9674187764171265 2316 64-65 -0.8799063169074302 2316 66-67 -0.9301572948260812 2316 68-69 -0.9633779074397282 2316 70-71 -1.116273421103994 2316 72-73 -1.3419989780337112 2316 74-75 -1.2898926297362365 2316 76-77 -1.2652919432232288 2316 78-79 -1.2648258278824542 2316 80-81 -1.3224395500417288 2316 82-83 -1.5234610282310754 2316 84-85 -1.459706512531703 2316 86-87 -1.741561434007643 2316 88-89 -1.9661349149212022 2316 90-91 -2.5240820266800483 2316 92-93 -2.6793108504769307 2316 94-95 -2.5889560291658356 2316 96-97 -2.68335603841944 2316 98-99 -3.208390638334439 2316 100-101 -3.7013754556205427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 29.0 5 93.0 6 226.0 7 570.0 8 1332.0 9 2479.0 10 4030.0 11 6362.0 12 8975.0 13 12504.0 14 14290.0 15 18126.0 16 23227.0 17 31379.0 18 29103.0 19 19428.0 20 17871.0 21 19095.0 22 20282.0 23 22864.0 24 27289.0 25 32314.0 26 39652.0 27 48737.0 28 59953.0 29 73808.0 30 89362.0 31 108762.0 32 132455.0 33 167596.0 34 219745.0 35 301972.0 36 454695.0 37 738573.0 38 702001.0 39 78489.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.68800806761867 20.436810103645225 15.131537013387602 22.743644815348507 2 15.413821675424519 21.927714190386858 39.4975070095425 23.16095712464612 3 18.34111887917621 26.010809903701883 31.286470385303023 24.361600831818887 4 12.960120373354696 18.086844907901515 37.83676064598628 31.116274072757506 5 13.229956932168946 37.94825000262211 34.64328366628483 14.178509398924113 6 30.10255149898333 37.438369743835274 18.187392263097873 14.271686494083525 7 26.871457845939457 33.757718575691584 21.938769580442276 17.432053997926687 8 25.415066115484603 35.321857838455806 20.757288549365576 18.505787496694012 9 26.077737534268163 16.97217811877514 21.25645358394485 35.69363076301184 10-11 24.158394258454045 27.11804973848333 28.24423964570593 20.479316357356698 12-13 25.315071529791016 24.81026541156808 28.43620657251443 21.438456486126476 14-15 22.370997851706367 25.921132097026277 27.101212452874528 24.606657598392825 16-17 21.454975361526333 29.093823500480354 27.60185145025829 21.84934968773502 18-19 21.31244409401008 27.76344433198481 30.021837264121192 20.902274309883918 20-21 22.41540815879629 26.80566132591885 29.783857111007077 20.995073404277786 22-23 22.020437731082456 26.925671777942 29.54102272562988 21.51286776534566 24-25 21.818747730455986 27.07072416489993 29.76998268839306 21.34054541625103 26-27 21.757406980099702 27.148008637957005 29.730352984931198 21.364231397012095 28-29 21.461531069472922 27.39351320736425 29.762527386894146 21.38242833626868 30-31 21.75765961361128 27.058775839493876 29.651576626184095 21.531987920710744 32-33 21.606597630007844 27.169655732318958 29.572814058827714 21.650932578845484 34-35 22.08205997584637 27.12088438575503 29.402934722828583 21.39412091557002 36-37 22.04700991129788 26.94350236994505 29.354898065995556 21.654589652761512 38-39 21.722001932638875 27.172783500361398 29.524992405667618 21.58022216133211 40-41 21.942564998404638 27.001370834465536 29.471934605908835 21.58412956122099 42-43 21.82086123381923 27.046375022784826 29.158326404572897 21.974437338823048 44-45 21.895981691952297 27.233830428313183 28.939692921222726 21.930494958511794 46-47 22.08257420898227 27.268649087959396 28.610949136235313 22.037827566823022 48-49 22.09096858128569 27.37255436666 28.45440947838253 22.082067573671782 50-51 21.882727292054966 27.522702629919138 28.399597468587668 22.194972609438228 52-53 22.134733455963122 27.596124490495338 28.125365855456163 22.143776198085376 54-55 21.777474274249077 27.394547878484822 28.25515329999141 22.57282454727469 56-57 21.978710720411737 27.67226494610781 28.08743736341987 22.261586970060588 58-59 22.051902420218404 27.35493819942441 28.19583139331756 22.39732798703963 60-61 21.99095662684619 27.44287582781363 28.368601222451336 22.197566322888846 62-63 21.832283496485378 27.414646010662498 28.338805751410767 22.414264741441357 64-65 21.663192722945404 27.435722119652773 28.211045128385674 22.69004002901615 66-67 21.652650249500446 27.523614169096756 27.990795002564028 22.832940578838766 68-69 21.605428751393983 27.3837317747562 28.130543685874663 22.880295787975154 70-71 21.6279481468872 27.370972796519805 28.07695900222771 22.92412005436529 72-73 21.754768203904483 27.21461431854434 28.0414866268049 22.98913085074628 74-75 21.54525438877725 27.570626225482904 27.75411735324891 23.13000203249094 76-77 21.567546432284455 27.32162841006597 27.978898904511347 23.13192625313824 78-79 21.546603419740645 27.37958973717675 27.877265120570833 23.196541722511768 80-81 21.457872278423636 27.43685738737173 27.808474613266647 23.296795720937986 82-83 21.524490098480847 27.35168698165457 27.788063070149853 23.33575984971473 84-85 21.428194816382728 27.229326491464246 27.67865722933216 23.663821462820863 86-87 21.43166894564838 27.141572104185023 27.805038969806578 23.621719980360016 88-89 21.397001882390814 27.0751730596572 27.676349361952273 23.851475695999714 90-91 21.30654384828344 27.085899432688908 27.755304914431456 23.852251804596193 92-93 21.372336749448863 27.097909085574674 27.664894686070863 23.864859478905608 94-95 21.24348777195732 26.877768897243108 27.69603796024623 24.18270537055334 96-97 21.24933029829719 26.811015140219236 27.703341279987754 24.23631328149582 98-99 21.057852687485614 26.80418654278558 27.556705759359403 24.58125501036941 100-101 21.117103106895797 26.531613368487804 27.969843542577266 24.381439982039133 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 876.0 1 636.0 2 479.0 3 603.5 4 602.0 5 629.5 6 943.0 7 1848.5 8 2922.5 9 3483.5 10 3303.5 11 3069.0 12 3273.5 13 3616.5 14 4001.5 15 4255.5 16 4573.5 17 4952.0 18 5358.5 19 5954.5 20 6761.5 21 7833.5 22 9162.5 23 10895.0 24 13046.0 25 16361.0 26 21433.5 27 27114.5 28 32239.0 29 37936.0 30 44785.0 31 51786.0 32 58840.0 33 65634.0 34 73764.0 35 80794.0 36 88025.0 37 96502.5 38 100552.0 39 102131.0 40 105179.0 41 111252.0 42 117882.0 43 123999.0 44 129586.0 45 133386.0 46 135243.5 47 139235.0 48 149003.0 49 150841.5 50 142003.5 51 132613.0 52 123034.5 53 116154.5 54 109741.0 55 99503.5 56 88577.5 57 77731.5 58 68360.5 59 59522.5 60 49562.0 61 39868.0 62 31851.5 63 24627.5 64 18074.0 65 13526.0 66 9856.0 67 7518.5 68 5588.0 69 3687.5 70 2565.5 71 1928.5 72 1495.0 73 1222.0 74 959.5 75 760.5 76 526.5 77 280.0 78 161.0 79 99.0 80 60.0 81 34.5 82 18.5 83 9.5 84 7.0 85 8.0 86 6.0 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37971012767274687 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.1260365491195232E-4 16-17 0.002211078011084304 18-19 1.559093469354317E-4 20-21 4.252073098239046E-5 22-23 0.0 24-25 0.0 26-27 1.4173576994130156E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007682078730818544 36-37 0.01715002816289749 38-39 0.011041216478427391 40-41 0.007866335231742237 42-43 0.0035150470945442785 44-45 0.0017575235472721393 46-47 0.001474052007389536 48-49 1.4173576994130155E-5 50-51 4.5355446381216495E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.8268657884151414E-4 60-61 7.086788497065078E-4 62-63 0.0 64-65 0.0 66-67 0.001190580467506933 68-69 8.22067465659549E-4 70-71 0.002055168664148872 72-73 0.0 74-75 0.0 76-77 0.096918919485862 78-79 0.0338890225929652 80-81 5.669430797652062E-5 82-83 0.0 84-85 0.0 86-87 0.1498005352509616 88-89 0.3236678042379562 90-91 0.2797297155561527 92-93 0.0 94-95 1.4173576994130155E-5 96-97 2.834715398826031E-5 98-99 1.984300779178222E-4 100-101 6.236373877417268E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3527691.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.015145374122895 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26645955015685 41.407952139474205 2 14.089537344515534 15.502758905253113 3 4.697392175792941 7.752831402915483 4 2.0700975379218303 4.555468679495335 5 1.0861228232865934 2.987660250863237 6 0.6454565449703181 2.1305931392532687 7 0.42343693049886644 1.6306811001717259 8 0.29002174966820066 1.2764470975722833 9 0.21859142724501504 1.082325523267936 >10 1.0728613236074516 10.860430646871773 >50 0.0854117158053716 3.2599051407576933 >100 0.051051136302485416 5.0804281260611255 >500 0.002631112346839446 0.9770410570228323 >1k 7.738565680684675E-4 0.6499888781770943 >5k 5.159043787123114E-5 0.20177157918697705 >10k+ 1.0318087574246228E-4 0.643716333656019 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12589 0.356862321558209 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10091 0.2860511308955348 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7109 0.20151991770254252 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3880 0.10998695747445 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.834715398826031E-5 2 0.0 0.0 0.0 0.0 8.504146196478092E-5 3 0.0 0.0 0.0 0.0 8.504146196478092E-5 4 0.0 0.0 0.0 0.0 1.1338861595304124E-4 5 0.0 0.0 0.0 0.0 1.1338861595304124E-4 6 0.0 0.0 0.0 0.0 1.1338861595304124E-4 7 0.0 0.0 0.0 0.0 1.1338861595304124E-4 8 0.0 0.0 0.0 0.0 1.1338861595304124E-4 9 0.0 0.0 0.0 2.834715398826031E-5 1.1338861595304124E-4 10-11 0.0 0.0 0.0 4.252073098239046E-5 1.1338861595304124E-4 12-13 0.0 0.0 0.0 7.086788497065077E-5 1.1338861595304124E-4 14-15 0.0 0.0 0.0 8.504146196478092E-5 1.1338861595304124E-4 16-17 0.0 0.0 0.0 1.4173576994130154E-4 1.4173576994130154E-4 18-19 0.0 0.0 0.0 1.84256500923692E-4 1.7008292392956185E-4 20-21 0.0 0.0 0.0 2.9764511687673325E-4 1.84256500923692E-4 22-23 0.0 0.0 0.0 6.661581187241173E-4 2.4095080890021263E-4 24-25 0.0 0.0 0.0 0.0013890105454247553 3.118186938708634E-4 26-27 0.0 0.0 0.0 0.0026221117439140787 3.68513001847384E-4 28-29 0.0 0.0 0.0 0.007015920612094426 3.68513001847384E-4 30-31 0.0 0.0 0.0 0.023173798385402802 3.968601558356443E-4 32-33 0.0 5.669430797652062E-5 0.0 0.05721873032530343 3.968601558356443E-4 34-35 0.0 5.669430797652062E-5 0.0 0.10783257377134223 3.968601558356443E-4 36-37 0.0 5.669430797652062E-5 0.0 0.18713373705350045 4.2520730982390463E-4 38-39 0.0 5.669430797652062E-5 0.0 0.30755244719563024 4.5355446381216495E-4 40-41 0.0 5.669430797652062E-5 0.0 0.447133833433824 4.5355446381216495E-4 42-43 0.0 5.669430797652062E-5 0.0 0.5892806371079553 4.5355446381216495E-4 44-45 0.0 5.669430797652062E-5 0.0 0.7595194703844526 4.6772804080629513E-4 46-47 0.0 5.669430797652062E-5 0.0 0.9571700015675976 4.8190161780042526E-4 48-49 0.0 5.669430797652062E-5 0.0 1.1712335349099452 4.8190161780042526E-4 50-51 0.0 5.669430797652062E-5 0.0 1.4020785834133431 4.8190161780042526E-4 52-53 0.0 5.669430797652062E-5 0.0 1.6379694253266512 4.960751947945554E-4 54-55 0.0 5.669430797652062E-5 0.0 1.8948796819222546 5.102487717886856E-4 56-57 0.0 5.669430797652062E-5 0.0 2.1925814931069643 5.102487717886856E-4 58-59 0.0 5.669430797652062E-5 0.0 2.5198352123244354 5.385959257769459E-4 60-61 0.0 5.669430797652062E-5 0.0 2.877448733463333 5.952902337534665E-4 62-63 0.0 5.669430797652062E-5 0.0 3.241454537826584 6.519845417299871E-4 64-65 0.0 5.669430797652062E-5 0.0 3.6165582529762386 6.661581187241173E-4 66-67 0.0 5.669430797652062E-5 0.0 4.045946768013412 6.945052727123776E-4 68-69 0.0 5.669430797652062E-5 0.0 4.516254399832638 7.086788497065078E-4 70-71 0.0 5.669430797652062E-5 0.0 5.030188301639798 7.086788497065078E-4 72-73 0.0 5.669430797652062E-5 0.0 5.554440567498684 7.086788497065078E-4 74-75 0.0 5.669430797652062E-5 0.0 6.102206797590831 7.086788497065078E-4 76-77 0.0 5.669430797652062E-5 0.0 6.68126828568602 7.086788497065078E-4 78-79 0.0 5.669430797652062E-5 0.0 7.3214178906259075 7.086788497065078E-4 80-81 0.0 5.669430797652062E-5 0.0 7.998106977056664 7.228524267006379E-4 82-83 0.0 5.669430797652062E-5 0.0 8.698621846414554 7.937203116712886E-4 84-85 0.0 5.669430797652062E-5 0.0 9.414543393965062 7.937203116712886E-4 86-87 0.0 5.669430797652062E-5 0.0 10.156771100416675 7.937203116712886E-4 88-89 0.0 5.669430797652062E-5 0.0 10.940272263075196 8.078938886654189E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19565 0.0 29.67665 1 GGTATCA 13440 0.0 28.387312 1 ATCAACG 23840 0.0 23.946432 3 TCAACGC 23950 0.0 23.836449 4 TATCAAC 24235 0.0 23.751947 2 CAACGCA 24320 0.0 23.51283 5 AACGCAG 24945 0.0 22.90469 6 GTACATG 14885 0.0 22.463598 1 AGTACTT 13415 0.0 21.225624 12-13 TACATGG 15480 0.0 21.029774 2 GAGTACT 12955 0.0 20.990227 12-13 ACGCAGA 27775 0.0 20.51967 7 CGCAGAG 27785 0.0 20.358572 8 ACATGGG 15375 0.0 20.216578 3 AGAGTAC 22805 0.0 19.748447 10-11 GCAGAGT 29120 0.0 19.164495 9 GTACTTT 14255 0.0 18.826582 14-15 CATGGGG 6155 0.0 18.581059 4 CATGGGA 9440 0.0 16.941013 4 TACTTTT 16655 0.0 16.897255 14-15 >>END_MODULE