FastQCFastQC Report
Thu 26 May 2016
SRR938748_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938748_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525528
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125870.3570245364665945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92120.2612941947986231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64920.18414263055065794No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA179600.030.7810381
GGTATCA121000.029.2671721
GTACATG143800.025.9490011
ATCAACG216050.025.1428283
TCAACGC216550.025.0628664
TATCAAC218050.025.0427572
TACATGG147700.024.9255142
CAACGCA219000.024.7608185
AACGCAG223500.024.2396766
ACATGGG147550.023.696883
ACGCAGA245250.022.0129177
CGCAGAG249200.021.5117028
GAGTACT115600.021.10510412-13
AGTACTT123050.020.5023612-13
GCAGAGT259550.020.1041249
AGAGTAC200350.020.02932410-11
CATGGGA88250.019.998214
CATGGGG64000.019.7180774
GTACTTT130350.018.68076914-15
AGTACAT110150.017.271212
Produced by FastQC (version 0.11.5)