##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938748_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3525528 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.38001740448523 33.0 31.0 34.0 28.0 34.0 2 31.451868769727543 33.0 31.0 34.0 28.0 34.0 3 31.463587581774984 33.0 31.0 34.0 28.0 34.0 4 35.0241938228827 37.0 35.0 37.0 32.0 37.0 5 35.061745928553115 37.0 35.0 37.0 32.0 37.0 6 35.110514510166986 37.0 35.0 37.0 32.0 37.0 7 35.01509646214695 37.0 35.0 37.0 32.0 37.0 8 34.98163679312716 37.0 35.0 37.0 32.0 37.0 9 36.522085202556895 39.0 37.0 39.0 32.0 39.0 10-11 36.39989627085645 39.0 37.0 39.0 32.0 39.0 12-13 36.286569841453534 39.0 37.0 39.0 32.0 39.0 14-15 37.406055631950736 40.0 37.0 41.0 32.0 41.0 16-17 37.28940360138964 40.0 37.0 41.0 31.5 41.0 18-19 37.23718603284387 40.0 37.0 41.0 31.0 41.0 20-21 37.12909853502794 40.0 37.0 41.0 31.0 41.0 22-23 37.12870781341121 40.0 37.0 41.0 31.0 41.0 24-25 37.06139222266849 40.0 37.0 41.0 31.0 41.0 26-27 36.932534928101546 40.0 36.0 41.0 30.0 41.0 28-29 36.75800617666347 39.5 36.0 41.0 30.0 41.0 30-31 36.5323265054199 39.0 36.0 41.0 30.0 41.0 32-33 36.34688804059988 39.0 35.5 41.0 30.0 41.0 34-35 36.05338944975051 39.0 35.0 41.0 28.5 41.0 36-37 35.84245537122383 39.0 35.0 41.0 27.0 41.0 38-39 35.681371839905964 39.0 35.0 40.0 27.0 41.0 40-41 35.570983693789984 39.0 35.0 40.5 27.0 41.0 42-43 35.56417804652239 39.0 35.0 41.0 26.5 41.0 44-45 35.38815037066789 39.0 35.0 41.0 26.0 41.0 46-47 35.089330165580876 39.0 35.0 41.0 24.5 41.0 48-49 34.80756527816543 39.0 34.0 40.5 24.0 41.0 50-51 33.68419865052837 38.0 32.5 39.5 22.0 40.5 52-53 33.76470403865747 38.0 33.0 39.5 22.5 40.5 54-55 34.27600518276979 38.0 34.0 40.0 23.0 41.0 56-57 34.18607283788414 38.0 34.0 40.0 22.0 41.0 58-59 33.98937010286119 38.0 33.5 40.0 21.5 41.0 60-61 33.69075383885761 38.0 33.0 40.0 20.0 41.0 62-63 33.3039642572687 37.0 33.0 40.0 18.5 41.0 64-65 32.9146766101418 37.0 32.0 40.0 17.0 41.0 66-67 32.53140068664892 36.0 32.0 39.0 13.5 41.0 68-69 32.09927846268701 36.0 31.0 39.0 10.0 40.5 70-71 31.673456287965948 35.0 31.0 38.5 8.0 40.0 72-73 31.240797264977047 35.0 31.0 38.0 7.0 40.0 74-75 30.753617188687763 35.0 30.0 37.0 2.0 39.0 76-77 30.27616586792106 35.0 30.0 36.5 2.0 39.0 78-79 29.820346058803107 34.0 29.0 36.0 2.0 38.5 80-81 29.328729342101383 34.0 29.0 35.5 2.0 37.5 82-83 28.891923564356887 34.0 29.0 35.0 2.0 37.0 84-85 28.523141923706177 34.0 29.0 35.0 2.0 36.5 86-87 28.07403188969142 34.0 27.5 35.0 2.0 36.0 88-89 27.597463840877168 34.0 26.5 35.0 2.0 36.0 90-91 27.11800303387181 33.5 26.0 35.0 2.0 35.0 92-93 26.623710264107956 33.0 25.0 35.0 2.0 35.0 94-95 26.078521429981553 33.0 24.0 35.0 2.0 35.0 96-97 25.438491624516953 32.5 20.5 35.0 2.0 35.0 98-99 24.62924957623369 32.0 9.5 35.0 2.0 35.0 100-101 22.19428409021287 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18267090420808785 1101 2 0.2526777705351897 1101 3 0.33747322229850596 1101 4 0.11950880840448264 1101 5 0.0809412539717016 1101 6 0.24710447105546507 1101 7 0.36369810508878686 1101 8 0.4302999247671906 1101 9 0.48907566580950146 1101 10-11 0.4159706056959962 1101 12-13 0.3530789859416572 1101 14-15 0.4135601893745431 1101 16-17 0.5197502558559677 1101 18-19 0.5792654046933023 1101 20-21 0.6937325619586048 1101 22-23 0.5908372007737484 1101 24-25 0.6819882718138075 1101 26-27 0.6231768644659468 1101 28-29 0.6689613308465923 1101 30-31 0.6457155050612471 1101 32-33 0.6426357567805283 1101 34-35 0.5022052969567241 1101 36-37 0.6178471901097637 1101 38-39 0.6409788317028955 1101 40-41 0.7503173323143031 1101 42-43 0.7534411594913735 1101 44-45 0.8499187319809494 1101 46-47 0.7231900822232191 1101 48-49 0.6272421543083908 1101 50-51 0.71703252252059 1101 52-53 0.7206296963717662 1101 54-55 0.8186391002937086 1101 56-57 0.8128155568092055 1101 58-59 0.7456659999063291 1101 60-61 0.7959622392647461 1101 62-63 0.7296564570833795 1101 64-65 0.684178799335541 1101 66-67 0.590159574075507 1101 68-69 0.6910867953172257 1101 70-71 0.6192247634543087 1101 72-73 0.6099650099137683 1101 74-75 0.42269823974528364 1101 76-77 0.2774409325301477 1101 78-79 0.2290922278089944 1101 80-81 0.05301009628585618 1101 82-83 0.14684035844646814 1101 84-85 0.15023209410304617 1101 86-87 -0.0806516043407548 1101 88-89 0.11178326658803428 1101 90-91 0.1755467295327584 1101 92-93 0.16521151722538718 1101 94-95 0.015195072811547305 1101 96-97 -0.21897767864570739 1101 98-99 -0.2823714203746235 1101 100-101 -0.05617714040973709 1102 1 0.2589274244761839 1102 2 0.21514432594475252 1102 3 0.2717980618993927 1102 4 0.3439017722230915 1102 5 0.3678737856727139 1102 6 0.427402727266454 1102 7 0.37488141569328803 1102 8 0.4175466124813809 1102 9 0.5860460373791625 1102 10-11 0.5535118081859949 1102 12-13 0.5476399718539326 1102 14-15 0.6879714971229021 1102 16-17 0.7956689053275952 1102 18-19 0.7853628283441623 1102 20-21 0.6984133775810974 1102 22-23 0.7148914635190167 1102 24-25 0.6495942410894102 1102 26-27 0.7690461332203427 1102 28-29 0.8495897518467856 1102 30-31 0.8085461628273478 1102 32-33 0.8211586475140109 1102 34-35 0.8827023785334944 1102 36-37 0.8095352882982993 1102 38-39 0.7877403886380989 1102 40-41 0.9846331426541539 1102 42-43 1.0354335437767261 1102 44-45 0.9599051206294078 1102 46-47 1.095146598547501 1102 48-49 1.1788373756498274 1102 50-51 1.2358564560818408 1102 52-53 1.0975764361858253 1102 54-55 1.045282892832418 1102 56-57 1.1135348360458437 1102 58-59 1.038426426627126 1102 60-61 1.0372303211383453 1102 62-63 0.9028662244605528 1102 64-65 0.8374601054440944 1102 66-67 0.8192295913245147 1102 68-69 0.9287129455101635 1102 70-71 0.9108316242397017 1102 72-73 0.7919653337217447 1102 74-75 0.6763401714259523 1102 76-77 0.656252681778227 1102 78-79 0.5906857211366798 1102 80-81 0.541900472754147 1102 82-83 0.511098521624934 1102 84-85 0.5168651086100589 1102 86-87 0.4774000575621997 1102 88-89 0.38278628775802304 1102 90-91 0.24539117715993441 1102 92-93 0.31811712304729056 1102 94-95 0.36091782364338343 1102 96-97 0.16822687251699264 1102 98-99 -0.031954984628715266 1102 100-101 0.402800306156756 1103 1 0.34051452950079053 1103 2 0.46209095570388214 1103 3 0.5187932542070861 1103 4 0.5214100278563478 1103 5 0.5149252295991573 1103 6 0.45241648665521694 1103 7 0.4243524886018619 1103 8 0.5245278835251526 1103 9 0.541906727614851 1103 10-11 0.5719271312888239 1103 12-13 0.5606719341046542 1103 14-15 0.6945567810519293 1103 16-17 0.7118954738979184 1103 18-19 0.6873929452180221 1103 20-21 0.6881848907901045 1103 22-23 0.7081331755107314 1103 24-25 0.7723816099705303 1103 26-27 0.7349228490970603 1103 28-29 0.7562434280317092 1103 30-31 0.9259349950599258 1103 32-33 0.8423784577963218 1103 34-35 0.8146035213721419 1103 36-37 0.9529556699999304 1103 38-39 0.9999526555378608 1103 40-41 1.028322237124506 1103 42-43 1.0909031031525345 1103 44-45 1.1301866620046965 1103 46-47 0.9984889159523149 1103 48-49 1.0061782928344982 1103 50-51 1.1242678132432005 1103 52-53 1.1349230594699478 1103 54-55 1.042644327693857 1103 56-57 1.0513171058593684 1103 58-59 1.037764761902963 1103 60-61 0.9843224361992142 1103 62-63 0.9857979050484857 1103 64-65 0.8860469352809233 1103 66-67 0.8322696473333195 1103 68-69 0.8400266029175683 1103 70-71 0.7671117734886046 1103 72-73 0.822268364024783 1103 74-75 0.6843408513016307 1103 76-77 0.7491447434202883 1103 78-79 0.7672753352262944 1103 80-81 0.7363459289183574 1103 82-83 0.674141208105123 1103 84-85 0.6990808962945962 1103 86-87 0.6344644919703839 1103 88-89 0.8838000309592644 1103 90-91 0.9005657190532226 1103 92-93 0.6930291034280209 1103 94-95 0.7043015810896414 1103 96-97 0.6912708135296803 1103 98-99 0.7084650409475621 1103 100-101 0.970293143180843 1104 1 0.144558147564247 1104 2 0.24502670181689012 1104 3 0.2395608167781873 1104 4 0.298008672149102 1104 5 0.20639320141055606 1104 6 0.25862184944121225 1104 7 0.3990287700454189 1104 8 0.4121556723634754 1104 9 0.47785232551650836 1104 10-11 0.37760218027124637 1104 12-13 0.4296464039883503 1104 14-15 0.5826403237456503 1104 16-17 0.6116320945843015 1104 18-19 0.4636780427088638 1104 20-21 0.6409691863911675 1104 22-23 0.507767387367636 1104 24-25 0.4138109686167226 1104 26-27 0.5314877587165796 1104 28-29 0.5371275411337209 1104 30-31 0.6119976175160033 1104 32-33 0.5089927843669173 1104 34-35 0.6364072203356059 1104 36-37 0.5958159871216608 1104 38-39 0.6102431134445752 1104 40-41 0.783755237851345 1104 42-43 0.7960165330286557 1104 44-45 0.7473697180342711 1104 46-47 0.8495062889140144 1104 48-49 0.8625022602532795 1104 50-51 0.9095549134833689 1104 52-53 0.9153366054818335 1104 54-55 0.7715581818210211 1104 56-57 0.716397275085761 1104 58-59 0.7082917575419501 1104 60-61 0.6809380070815294 1104 62-63 0.6969195829337096 1104 64-65 0.7548964802183562 1104 66-67 0.7282121048292112 1104 68-69 0.6787840490368993 1104 70-71 0.7804461536238527 1104 72-73 0.6839009009986157 1104 74-75 0.5028550246046279 1104 76-77 0.5184888528881189 1104 78-79 0.42935977461448616 1104 80-81 0.45579758247037105 1104 82-83 0.5649706307444831 1104 84-85 0.49242886510384665 1104 86-87 0.5818088740017338 1104 88-89 0.8094109287597959 1104 90-91 0.677484822990781 1104 92-93 0.5933404776221813 1104 94-95 0.6858097263936465 1104 96-97 0.5755048902898885 1104 98-99 0.603673749549511 1104 100-101 0.9189815793772773 1105 1 0.11515998437615949 1105 2 0.2502630426144208 1105 3 0.22385752725672958 1105 4 0.17632051995538944 1105 5 0.30629962821389967 1105 6 0.2087420486393725 1105 7 0.28711643711083923 1105 8 0.21376043039172288 1105 9 0.2683859539016069 1105 10-11 0.29926663659760067 1105 12-13 0.37679012834171743 1105 14-15 0.38172644038792214 1105 16-17 0.5061609608644488 1105 18-19 0.44654997546355446 1105 20-21 0.4056857850914568 1105 22-23 0.3580660381045675 1105 24-25 0.4239904775517189 1105 26-27 0.4521110619459705 1105 28-29 0.4902592493891831 1105 30-31 0.5616888068574468 1105 32-33 0.4990351056956186 1105 34-35 0.4497172272532737 1105 36-37 0.5859271029040016 1105 38-39 0.6347540039887747 1105 40-41 0.5613004096909648 1105 42-43 0.6424743193505336 1105 44-45 0.6754825396642659 1105 46-47 0.7378438136131322 1105 48-49 0.7541293382598226 1105 50-51 0.6426424658913632 1105 52-53 0.7251169154900197 1105 54-55 0.7115221220615666 1105 56-57 0.5739978519399926 1105 58-59 0.6439912378890327 1105 60-61 0.6764969429077681 1105 62-63 0.649549505718312 1105 64-65 0.5816306418878625 1105 66-67 0.5546637633488274 1105 68-69 0.59157688727948 1105 70-71 0.5298894281379312 1105 72-73 0.644590125876217 1105 74-75 0.5606375882681611 1105 76-77 0.4917894387875492 1105 78-79 0.5484022067787002 1105 80-81 0.7195610913442181 1105 82-83 0.7368890851114678 1105 84-85 0.7183312190207367 1105 86-87 0.7726601352970839 1105 88-89 0.7260170817866864 1105 90-91 0.7600147581329004 1105 92-93 0.7370632385219587 1105 94-95 0.8143612400627234 1105 96-97 0.6467587456457302 1105 98-99 0.5970440025729609 1105 100-101 1.0749696588272286 1106 1 0.09052641187428279 1106 2 0.22717932202525404 1106 3 0.23191004573501672 1106 4 0.22838877061131058 1106 5 0.24958026823934887 1106 6 0.20067523722043035 1106 7 0.3036086263294919 1106 8 0.13768501089391805 1106 9 0.15197026298707073 1106 10-11 0.28257046225852633 1106 12-13 0.26955330177226244 1106 14-15 0.4803607809799928 1106 16-17 0.44657058411495854 1106 18-19 0.3851543381661884 1106 20-21 0.3837844946253526 1106 22-23 0.3939824890306838 1106 24-25 0.2190857928229164 1106 26-27 0.1744293644914805 1106 28-29 0.14236581134038317 1106 30-31 0.11509997250269066 1106 32-33 0.25192694044934427 1106 34-35 0.3685729406387992 1106 36-37 0.44114443017381433 1106 38-39 0.4032343101808493 1106 40-41 0.5675987804200204 1106 42-43 0.5516063277075531 1106 44-45 0.5680811889192299 1106 46-47 0.6695498127953314 1106 48-49 0.6581186844261424 1106 50-51 0.6854973627149761 1106 52-53 0.3777746878047168 1106 54-55 0.3040642520577279 1106 56-57 0.4575714413199705 1106 58-59 0.6176981306907336 1106 60-61 0.5438879716639207 1106 62-63 0.5028389949383225 1106 64-65 0.5360538720178809 1106 66-67 0.5617081634368262 1106 68-69 0.6139931896833062 1106 70-71 0.541569712455594 1106 72-73 0.5577474613240803 1106 74-75 0.4590105855475315 1106 76-77 0.48267256230465705 1106 78-79 0.5559617741624585 1106 80-81 0.5024836372773791 1106 82-83 0.4863088227925054 1106 84-85 0.7321686498708679 1106 86-87 0.6871453160033489 1106 88-89 0.7344858044825102 1106 90-91 0.7510644254802408 1106 92-93 0.8920821424895564 1106 94-95 0.8994432318275045 1106 96-97 1.0429500498206359 1106 98-99 1.0857771260279243 1106 100-101 1.523868356678193 1107 1 0.0393942627788455 1107 2 0.14237443414501172 1107 3 0.21500404055313282 1107 4 0.2275753816429713 1107 5 0.2303325679076167 1107 6 0.06559192888849452 1107 7 0.09801110524088585 1107 8 0.1349004996113834 1107 9 0.21029223065520597 1107 10-11 0.22587527252706963 1107 12-13 0.16525967249602758 1107 14-15 0.2346492872708339 1107 16-17 0.2887831708299231 1107 18-19 0.29606615901018074 1107 20-21 0.2459300193141516 1107 22-23 0.24625852100547263 1107 24-25 0.17539907473006622 1107 26-27 0.21212019569739482 1107 28-29 0.14161906719699857 1107 30-31 0.02617813698916649 1107 32-33 0.04517091910091153 1107 34-35 0.17973532761867972 1107 36-37 0.15475267456046282 1107 38-39 0.06535049274073401 1107 40-41 0.16153853411179142 1107 42-43 0.11146220002925844 1107 44-45 0.3258036292517943 1107 46-47 0.38898867410599536 1107 48-49 0.16657207047407496 1107 50-51 0.1988774041774164 1107 52-53 0.17636083586574358 1107 54-55 0.2680105521272438 1107 56-57 0.46384863225516426 1107 58-59 0.45610275941540124 1107 60-61 0.35488930670778274 1107 62-63 0.39909815725965814 1107 64-65 0.43837211493819694 1107 66-67 0.24139373177223433 1107 68-69 0.355709905156381 1107 70-71 0.4391919109955147 1107 72-73 0.3294546547931638 1107 74-75 0.2870132531811258 1107 76-77 0.20670071356953912 1107 78-79 0.5203156890129179 1107 80-81 0.41113820020083125 1107 82-83 0.32986864729804566 1107 84-85 0.5328180266152174 1107 86-87 0.5446855222357243 1107 88-89 0.5544355685490885 1107 90-91 0.6565492562284589 1107 92-93 0.7902750784142007 1107 94-95 0.7261929920901942 1107 96-97 0.8078990439055822 1107 98-99 1.0474493727607204 1107 100-101 1.427738986879021 1108 1 0.24231458830482566 1108 2 0.27465232308108867 1108 3 0.18984399611575853 1108 4 0.19111043283010787 1108 5 0.34303970462713096 1108 6 0.21942025799035036 1108 7 0.27503106070946615 1108 8 0.2699504543616058 1108 9 0.34063615978242723 1108 10-11 0.42014795267122196 1108 12-13 0.4998512188547082 1108 14-15 0.623174256375961 1108 16-17 0.6783016729706404 1108 18-19 0.6784452336185538 1108 20-21 0.6196456788185216 1108 22-23 0.6356796076131133 1108 24-25 0.6568957178467443 1108 26-27 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-2.5515672244558054 2316 72-73 -2.4204461087977442 2316 74-75 -2.4358464713353456 2316 76-77 -2.3630009853143434 2316 78-79 -2.306948815250312 2316 80-81 -2.3480160530906673 2316 82-83 -2.2705368780114483 2316 84-85 -2.2339108426816097 2316 86-87 -2.3921828807830146 2316 88-89 -2.526326374301597 2316 90-91 -2.8191424391909727 2316 92-93 -2.711871061656023 2316 94-95 -2.757349918764831 2316 96-97 -2.8081411029914563 2316 98-99 -3.178825591101482 2316 100-101 -3.2868346332326013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18663.0 3 4536.0 4 4287.0 5 4869.0 6 5683.0 7 6739.0 8 8464.0 9 9928.0 10 11879.0 11 14650.0 12 17834.0 13 21354.0 14 22542.0 15 24621.0 16 29010.0 17 35384.0 18 32057.0 19 24870.0 20 25609.0 21 27990.0 22 30930.0 23 34485.0 24 39381.0 25 45000.0 26 51932.0 27 59702.0 28 69232.0 29 80306.0 30 96047.0 31 114809.0 32 138853.0 33 172259.0 34 223530.0 35 303329.0 36 435247.0 37 631389.0 38 562539.0 39 85575.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.818403595413066 19.582327046404345 14.566844141027978 23.03242521715461 2 14.965398833245871 21.173947965141142 40.336348854540425 23.52430434707256 3 18.403028922492425 25.666520112623985 30.91771156288661 25.01273940199698 4 12.587410799291195 17.322545387701503 38.34741916356676 31.742624649440536 5 13.335060099420785 37.69181603856735 34.57334472127038 14.399779140741481 6 30.269628147615553 36.790352214645324 18.51253362358916 14.42748601414997 7 26.98738185146359 33.48971319081158 22.08453212710441 17.438372830620427 8 25.553152515808215 34.56785946027715 21.134699824444773 18.744288199469867 9 26.441903710029024 16.698823546790248 21.36762188508631 35.49165085809442 10-11 24.207090122290044 26.733356545466176 28.482125473420922 20.577427858822862 12-13 25.31946490011184 24.62215857692889 28.472720931511653 21.585655591447622 14-15 22.48021355579202 25.66229714543871 27.211727383927343 24.64576191484193 16-17 21.600380606153703 28.812127625378864 27.641863263399085 21.94562850506835 18-19 21.39307322791116 27.604241962380254 30.005587963659895 20.997096846048688 20-21 22.53526370634517 26.698598603306984 29.73020943005813 21.035928260289715 22-23 22.029100932289847 26.845202011298486 29.464786353560434 21.660910702851236 24-25 21.874032929269237 26.88542277471561 29.72427689584955 21.516267400165606 26-27 21.770151491605716 27.023303510896092 29.669995007772958 21.536549989725234 28-29 21.49188737618656 27.302436988203883 29.665075241978784 21.540600393630775 30-31 21.780942603306055 26.91402201856054 29.642759508628973 21.662275869504427 32-33 21.57617571126256 27.113681371935368 29.485799899425814 21.824343017376258 34-35 22.102527081567228 27.06398918729963 29.317892366035416 21.515591365097727 36-37 22.068601294577363 26.838651417124936 29.230041291547316 21.862705996750385 38-39 21.78276908718156 27.11621668908618 29.418196918820104 21.68281730491216 40-41 22.007505640899538 26.948999096089963 29.371910597149835 21.67158466586066 42-43 21.81188076528869 27.02045609839426 29.049538776904683 22.118124359412366 44-45 21.891284174967403 27.14440366089537 28.925577948068316 22.03873421606891 46-47 21.992193884611638 27.24624430003229 28.558667738083106 22.20289407727297 48-49 22.05754105233418 27.32674513717529 28.465524469629134 22.1501893408614 50-51 21.88083103414494 27.492279397525305 28.36378761195016 22.263101956379593 52-53 22.115580133406162 27.573858383963923 28.150454721246504 22.16010676138341 54-55 21.800610599462185 27.333936851565554 28.362703826227754 22.502748722744503 56-57 21.975318694499553 27.608208652991085 28.158755705297395 22.257716947211968 58-59 22.080932308497587 27.2600871506017 28.274605087717724 22.384375453182987 60-61 21.992379931857283 27.24973186357059 28.538949172657386 22.218939031914744 62-63 21.873895780154008 27.22202844740817 28.51698202924465 22.387093743193166 64-65 21.679601132435703 27.292774278060843 28.448238662006624 22.57938592749683 66-67 21.701483962091764 27.38418727891289 28.177562153632866 22.736766605362476 68-69 21.68354331940073 27.15417674722281 28.38076429060374 22.78151564277272 70-71 21.629224325460303 27.158272792104455 28.34312281218002 22.869380070255218 72-73 21.753123201987002 26.938165442831018 28.391330230544433 22.917381124637547 74-75 21.525166012500016 27.243680530841246 28.15910084875755 23.072052607901185 76-77 21.630817541734828 27.032413505677198 28.29897832734903 23.037790625238944 78-79 21.54596968052192 27.052910687831684 28.289667072788298 23.1114525588581 80-81 21.51168957116196 27.092052338732834 28.20429810396023 23.191959986144976 82-83 21.574625836073082 26.966427795762744 28.254539536715455 23.204406831448722 84-85 21.481019429426038 26.79471351488499 28.19817527617247 23.5260917795165 86-87 21.489519828467454 26.71010031692517 28.360503093793337 23.439876760814037 88-89 21.5501532221067 26.51790410497416 28.278028995016186 23.653913677902956 90-91 21.44427912866892 26.560250906526868 28.432294217582683 23.56317574722153 92-93 21.604742140688234 26.431891471492623 28.384484021465646 23.578882366353497 94-95 21.423286299037734 26.24285238369719 28.370070731615954 23.963790585649118 96-97 21.50057755058731 26.068938603025483 28.44414565953998 23.986338186847227 98-99 21.388778786133862 25.95075014751712 28.324753863965785 24.335717202383233 100-101 21.604479030494534 25.46671874223547 28.772383014005015 24.156419213264975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3202.0 1 2148.5 2 1068.5 3 1101.5 4 1102.5 5 1155.0 6 1532.5 7 2463.0 8 3607.5 9 4095.5 10 3867.5 11 3711.0 12 3867.5 13 4222.5 14 4596.0 15 4829.0 16 5130.5 17 5505.5 18 5947.5 19 6421.0 20 7039.5 21 8025.0 22 9392.0 23 11225.0 24 13522.5 25 16745.5 26 21519.5 27 26961.0 28 32251.5 29 37890.5 30 44406.5 31 51097.5 32 57896.0 33 64747.0 34 73003.0 35 80472.5 36 87708.0 37 95971.0 38 100408.0 39 101828.5 40 104359.0 41 110063.0 42 116402.5 43 122691.5 44 128182.0 45 132069.0 46 133882.0 47 136936.0 48 146386.5 49 148331.5 50 139148.5 51 129827.0 52 121095.0 53 114918.0 54 108675.0 55 98984.5 56 88862.5 57 78208.5 58 68551.0 59 59604.0 60 49932.5 61 41187.5 62 33608.5 63 26137.0 64 19339.5 65 14523.5 66 10795.0 67 8431.0 68 6379.5 69 4384.0 70 3295.5 71 2485.5 72 1871.0 73 1467.5 74 1198.5 75 989.0 76 701.0 77 451.5 78 283.0 79 197.0 80 153.0 81 117.0 82 79.5 83 52.5 84 44.5 85 38.0 86 27.5 87 21.0 88 18.5 89 10.5 90 8.0 91 9.0 92 8.5 93 7.0 94 4.0 95 2.5 96 1.5 97 3.5 98 3.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27556156127536074 2 0.16590422767880442 3 0.19619756246440248 4 0.16442927130347568 5 0.23701414369705756 6 0.19268035880015702 7 0.23970877553660044 8 0.24637444377125922 9 0.14993498846130282 10-11 0.20572804981268053 12-13 0.2104223821226211 14-15 0.17225788591098978 16-17 0.28599971408537955 18-19 0.23107177137722348 20-21 0.1986227311199911 22-23 0.2438216346601133 24-25 0.23090158410314712 26-27 0.3422466081676277 28-29 0.31391042703390815 30-31 0.28991402138913663 32-33 0.1909643037865534 34-35 0.35801729556537343 36-37 0.3980822163375245 38-39 0.3782270343619452 40-41 0.337651551767565 42-43 0.3723697556791493 44-45 0.38266608576077116 46-47 0.38762988125466596 48-49 0.4084494577833448 50-51 0.4011881340894186 52-53 0.40124486318077746 54-55 0.3941820913066071 56-57 0.3719301052211187 58-59 0.34560780683063647 60-61 0.31179726838079286 62-63 0.381020942111366 64-65 0.4166326292118514 66-67 0.4224189965304488 68-69 0.42708496429470993 70-71 0.4266453138366792 72-73 0.4171857378525996 74-75 0.4133423419130411 76-77 0.3966072599621957 78-79 0.36968930611244616 80-81 0.3813471343866791 82-83 0.39138818355718635 84-85 0.3919554744707743 86-87 0.38740296488923076 88-89 0.3959406931387298 90-91 0.41805085649582135 92-93 0.4171999201254393 94-95 0.42559582564654147 96-97 0.42583692428481634 98-99 0.42315647471811313 100-101 0.3960966981399665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3525528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.25277662788125 #Duplication Level Percentage of deduplicated Percentage of total 1 76.90359030489483 44.02944077608238 2 13.308054110015025 15.238460986248944 3 4.345432162055481 7.463641707773207 4 1.921254434710221 4.399886039831621 5 1.0007803613692448 2.8648727241521827 6 0.5887829845689241 2.0225676418693115 7 0.3642861221080126 1.4599474384380984 8 0.26695746865471026 1.222724505762619 9 0.1821453676877121 0.9385495245025091 >10 0.9918524896637273 10.37421424243524 >50 0.07900014228822683 3.138272050997041 >100 0.04463198721148388 4.584952209528367 >500 0.0025856518223499408 0.9926081449985514 >1k 4.972407307280208E-4 0.4644621497197897 >5k 9.944814614560414E-5 0.447067949690443 >10k+ 4.972407307280207E-5 0.3583319079695368 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12587 0.3570245364665945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9212 0.2612941947986231 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6492 0.18414263055065794 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.8364545679398943E-5 10-11 0.0 0.0 0.0 5.6729091358797885E-5 2.8364545679398943E-5 12-13 0.0 0.0 0.0 1.1345818271759577E-4 2.8364545679398943E-5 14-15 0.0 1.4182272839699471E-5 0.0 1.1345818271759577E-4 2.8364545679398943E-5 16-17 0.0 2.8364545679398943E-5 0.0 1.418227283969947E-4 2.8364545679398943E-5 18-19 0.0 2.8364545679398943E-5 0.0 1.560050012366942E-4 5.6729091358797885E-5 20-21 0.0 2.8364545679398943E-5 0.0 2.978277296336889E-4 5.6729091358797885E-5 22-23 0.0 2.8364545679398943E-5 0.0 5.531086407482794E-4 7.091136419849735E-5 24-25 0.0 2.8364545679398943E-5 0.0 0.0012764045555729523 8.509363703819683E-5 26-27 0.0 2.8364545679398943E-5 0.0 0.002481897746947408 8.509363703819683E-5 28-29 0.0 2.8364545679398943E-5 0.0 0.007474057786521621 9.92759098778963E-5 30-31 0.0 2.8364545679398943E-5 0.0 0.02337238563982473 1.2764045555729525E-4 32-33 0.0 2.8364545679398943E-5 0.0 0.05657308635756119 1.7018727407639366E-4 34-35 0.0 2.8364545679398943E-5 0.0 0.10411206491623382 1.7018727407639366E-4 36-37 0.0 2.8364545679398943E-5 0.0 0.17898028323700735 1.7018727407639366E-4 38-39 0.0 2.8364545679398943E-5 0.0 0.2917435345854579 1.8436954691609313E-4 40-41 0.0 2.8364545679398943E-5 0.0 0.41989455196498227 1.9855181975579262E-4 42-43 0.0 2.8364545679398943E-5 0.0 0.5544134098495317 1.9855181975579262E-4 44-45 0.0 2.8364545679398943E-5 0.0 0.718062656146824 2.1273409259549208E-4 46-47 0.0 2.8364545679398943E-5 0.0 0.905736672634567 2.2691636543519154E-4 48-49 0.0 2.8364545679398943E-5 0.0 1.1138189797386377 2.2691636543519154E-4 50-51 0.0 2.8364545679398943E-5 0.0 1.3322968928342083 2.2691636543519154E-4 52-53 0.0 4.2546818519098416E-5 0.0 1.5547458423248943 2.2691636543519154E-4 54-55 0.0 5.6729091358797885E-5 0.0 1.8022406856504898 2.2691636543519154E-4 56-57 0.0 5.6729091358797885E-5 0.0 2.091374682033443 2.2691636543519154E-4 58-59 0.0 5.6729091358797885E-5 0.0 2.4073273563562676 2.2691636543519154E-4 60-61 0.0 5.6729091358797885E-5 0.0 2.7497725163436515 2.2691636543519154E-4 62-63 0.0 5.6729091358797885E-5 0.0 3.102684193686733 2.2691636543519154E-4 64-65 0.0 5.6729091358797885E-5 0.0 3.4696363211411168 2.2691636543519154E-4 66-67 0.0 5.6729091358797885E-5 0.0 3.8844252548838076 2.2691636543519154E-4 68-69 0.0 5.6729091358797885E-5 0.0 4.348540133562973 2.2691636543519154E-4 70-71 0.0 5.6729091358797885E-5 0.0 4.853684327567389 2.4109863827489103E-4 72-73 0.0 5.6729091358797885E-5 0.0 5.364345425706448 4.2546818519098416E-4 74-75 0.0 5.6729091358797885E-5 0.0 5.895982105375422 4.2546818519098416E-4 76-77 0.0 5.6729091358797885E-5 0.0 6.461840609406591 4.2546818519098416E-4 78-79 0.0 5.6729091358797885E-5 0.0 7.090781863028743 4.2546818519098416E-4 80-81 0.0 5.6729091358797885E-5 0.0 7.762113930168757 4.396504580306836E-4 82-83 0.0 5.6729091358797885E-5 0.0 8.44673478695957 4.8219727654978206E-4 84-85 0.0 5.6729091358797885E-5 0.0 9.141226505646813 4.8219727654978206E-4 86-87 0.0 5.6729091358797885E-5 0.0 9.869769861422174 4.8219727654978206E-4 88-89 0.0 5.6729091358797885E-5 0.0 10.640477114349965 4.8219727654978206E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 17960 0.0 30.781038 1 GGTATCA 12100 0.0 29.267172 1 GTACATG 14380 0.0 25.949001 1 ATCAACG 21605 0.0 25.142828 3 TCAACGC 21655 0.0 25.062866 4 TATCAAC 21805 0.0 25.042757 2 TACATGG 14770 0.0 24.925514 2 CAACGCA 21900 0.0 24.760818 5 AACGCAG 22350 0.0 24.239676 6 ACATGGG 14755 0.0 23.69688 3 ACGCAGA 24525 0.0 22.012917 7 CGCAGAG 24920 0.0 21.511702 8 GAGTACT 11560 0.0 21.105104 12-13 AGTACTT 12305 0.0 20.50236 12-13 GCAGAGT 25955 0.0 20.104124 9 AGAGTAC 20035 0.0 20.029324 10-11 CATGGGA 8825 0.0 19.99821 4 CATGGGG 6400 0.0 19.718077 4 GTACTTT 13035 0.0 18.680769 14-15 AGTACAT 11015 0.0 17.27121 2 >>END_MODULE