FastQCFastQC Report
Thu 26 May 2016
SRR938748_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938748_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3525528
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123630.3506708782344091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99480.2821705004186607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68290.19370148244461538No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38650.10962896905087692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135750.030.5578861
GTATCAA193350.027.900731
GTACATG152550.023.0139331
ATCAACG234450.022.7324393
TCAACGC234850.022.7139264
TATCAAC236650.022.5612162
CAACGCA239050.022.3347075
TACATGG155000.022.2903142
AACGCAG244450.021.8539186
ACATGGG151750.021.67313
GAGTACT125050.020.96214512-13
AGTACTT130800.019.80487312-13
ACGCAGA270850.019.6006247
CATGGGG63950.019.4436244
CGCAGAG273150.019.3487388
AGAGTAC220800.018.87686210-11
GTACTTT139250.018.67121114-15
GCAGAGT287900.018.0113879
CATGGGA91450.017.2294274
GTACACG6150.016.2439211
Produced by FastQC (version 0.11.5)