##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938747_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2871836 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.643160333668078 33.0 31.0 34.0 30.0 34.0 2 31.649921513624037 33.0 31.0 34.0 30.0 34.0 3 31.668769734762012 33.0 31.0 34.0 28.0 34.0 4 35.199275655016514 37.0 35.0 37.0 33.0 37.0 5 35.26054447398807 37.0 35.0 37.0 33.0 37.0 6 35.383728040180564 37.0 35.0 37.0 33.0 37.0 7 35.313540536437316 37.0 35.0 37.0 33.0 37.0 8 35.301221936071556 37.0 35.0 37.0 32.0 37.0 9 36.82821581733776 39.0 37.0 39.0 33.0 39.0 10-11 36.72322200849909 39.0 37.0 39.0 32.0 39.0 12-13 36.61214115290706 39.0 37.0 39.0 32.0 39.0 14-15 37.64081462172631 40.0 37.0 41.0 32.0 41.0 16-17 37.60818027213253 40.0 37.0 41.0 32.0 41.0 18-19 37.63881259236251 40.0 37.0 41.0 32.0 41.0 20-21 37.53902990978594 40.0 37.0 41.0 32.0 41.0 22-23 37.463922556859096 40.0 37.0 41.0 32.0 41.0 24-25 37.46113966814261 40.0 37.0 41.0 32.0 41.0 26-27 37.300469978090675 40.0 36.5 41.0 31.5 41.0 28-29 37.16846505162551 40.0 36.5 41.0 31.0 41.0 30-31 36.97128840226252 39.0 36.0 41.0 31.0 41.0 32-33 36.71213310927226 39.0 35.0 41.0 30.0 41.0 34-35 36.358000422029676 39.0 35.0 41.0 30.0 41.0 36-37 36.04495416869209 39.0 35.0 41.0 30.0 41.0 38-39 35.80891979904145 38.5 35.0 40.5 28.5 41.0 40-41 35.62830311340898 38.0 35.0 40.0 27.5 41.0 42-43 35.37214590248189 38.0 35.0 40.0 26.5 41.0 44-45 35.245821140204384 39.0 35.0 40.5 25.5 41.0 46-47 34.929030766380805 39.0 35.0 41.0 24.0 41.0 48-49 34.61841588447251 38.5 34.0 40.0 23.0 41.0 50-51 33.43128455106768 37.5 32.5 39.5 21.5 40.5 52-53 33.51293823881308 37.5 33.0 39.5 21.0 40.5 54-55 34.048071512440124 38.0 34.0 40.0 22.0 41.0 56-57 33.96441823279602 38.0 34.0 40.0 20.5 41.0 58-59 33.76516242570955 38.0 33.5 40.0 19.5 41.0 60-61 33.40394942468859 37.5 33.0 40.0 18.0 41.0 62-63 33.00936299983704 37.0 33.0 40.0 14.0 41.0 64-65 32.56666240690625 36.0 32.0 39.0 12.0 41.0 66-67 32.116149216041585 36.0 31.5 39.0 8.0 41.0 68-69 31.726025093354913 35.0 31.0 39.0 7.0 40.0 70-71 31.241644369664563 35.0 31.0 37.5 3.5 40.0 72-73 30.762509593166182 35.0 30.5 37.0 2.0 39.0 74-75 30.322747364403817 35.0 30.0 37.0 2.0 39.0 76-77 29.843857030833234 34.5 29.5 36.0 2.0 38.5 78-79 29.41394320567052 34.0 29.0 36.0 2.0 37.5 80-81 28.980165127813706 34.0 29.0 35.0 2.0 37.0 82-83 28.523106124444432 34.0 28.5 35.0 2.0 36.5 84-85 28.11220800909244 34.0 27.5 35.0 2.0 36.0 86-87 27.7382744000702 34.0 27.0 35.0 2.0 36.0 88-89 27.285149987673393 34.0 26.0 35.0 2.0 35.0 90-91 26.801290707408082 33.0 25.0 35.0 2.0 35.0 92-93 26.370299522674692 33.0 24.5 35.0 2.0 35.0 94-95 25.88027954938931 33.0 23.5 35.0 2.0 35.0 96-97 25.23461332750199 33.0 19.0 35.0 2.0 35.0 98-99 24.51288356995316 32.0 4.5 35.0 2.0 35.0 100-101 22.284883607559763 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3502302282687282 1101 2 0.18650294068885387 1101 3 0.30020937520533764 1101 4 0.3767400400141625 1101 5 0.1785123218007243 1101 6 0.17451844516923387 1101 7 0.3892216514466682 1101 8 0.4878490455023936 1101 9 0.5821051987330463 1101 10-11 0.5541869619474014 1101 12-13 0.48890572780894104 1101 14-15 0.7585359132625413 1101 16-17 0.6500778296916465 1101 18-19 0.7239844167783573 1101 20-21 0.7128548078571839 1101 22-23 0.7248933895193446 1101 24-25 0.5959680594576895 1101 26-27 0.7053053723168929 1101 28-29 0.6008167529532074 1101 30-31 0.5946624864553272 1101 32-33 0.702253126939901 1101 34-35 0.6928568716351649 1101 36-37 0.7494028956619729 1101 38-39 0.7681653300758811 1101 40-41 0.7675456552802302 1101 42-43 0.8326024633752169 1101 44-45 0.9703441795433108 1101 46-47 1.0757899917621643 1101 48-49 1.0210370303994765 1101 50-51 1.0948297897124561 1101 52-53 0.9683900856357823 1101 54-55 0.9917517410427124 1101 56-57 1.0626827408824937 1101 58-59 0.9387678007338138 1101 60-61 0.8408591358860846 1101 62-63 0.9101618422540412 1101 64-65 0.8786890177201414 1101 66-67 0.7688976420998657 1101 68-69 0.6792547600704637 1101 70-71 0.7347037799913352 1101 72-73 0.743576695527782 1101 74-75 0.6850859642973219 1101 76-77 0.5947087888738452 1101 78-79 0.507048768371579 1101 80-81 0.4487887778413153 1101 82-83 0.34408774379173224 1101 84-85 0.3329157533095781 1101 86-87 0.41204195947099365 1101 88-89 0.10003182563932 1101 90-91 0.2648169890230605 1101 92-93 0.07549908946948847 1101 94-95 0.12762844741261858 1101 96-97 -0.059417801692429606 1101 98-99 -0.10455563156012104 1101 100-101 0.147744172467128 1102 1 0.20828733588644255 1102 2 0.2752422350041428 1102 3 0.34579153585307765 1102 4 0.2532167732585364 1102 5 0.27949636760590124 1102 6 0.22467713651058574 1102 7 0.263788506487316 1102 8 0.1881483527894332 1102 9 0.3483858139344562 1102 10-11 0.316524482747397 1102 12-13 0.3092598194937253 1102 14-15 0.2669458898176913 1102 16-17 0.24138808322565097 1102 18-19 0.3724761844989004 1102 20-21 0.35416506139119264 1102 22-23 0.335772119563309 1102 24-25 0.44670968230924046 1102 26-27 0.3201665373293068 1102 28-29 0.4374211569702027 1102 30-31 0.49542165307326513 1102 32-33 0.4214765760443768 1102 34-35 0.34890083206067857 1102 36-37 0.4999448917172913 1102 38-39 0.5052798323911176 1102 40-41 0.488919411932244 1102 42-43 0.5533690993845966 1102 44-45 0.5736196804710048 1102 46-47 0.44104682186939215 1102 48-49 0.49988057721741086 1102 50-51 0.41538536889597566 1102 52-53 0.42407612664566585 1102 54-55 0.4605100145234502 1102 56-57 0.4093411850162312 1102 58-59 0.29768747686525643 1102 60-61 0.34620732648581765 1102 62-63 0.2945563962436495 1102 64-65 0.4085590077786705 1102 66-67 0.4575744699742543 1102 68-69 0.39556208964340556 1102 70-71 0.3106281504093502 1102 72-73 0.29166708313869094 1102 74-75 0.18006419407813112 1102 76-77 0.25705096143007466 1102 78-79 0.10218941089872757 1102 80-81 0.018205075468653575 1102 82-83 0.008635912063034823 1102 84-85 -0.1587610586159265 1102 86-87 -0.14434064880859765 1102 88-89 -0.18172531547103787 1102 90-91 0.014091580864679543 1102 92-93 -0.03549845971951271 1102 94-95 -0.1611604213334239 1102 96-97 -0.18453183617390323 1102 98-99 -0.44469457287356207 1102 100-101 -0.23612340399445486 1103 1 0.13537991690410323 1103 2 0.2312866450769242 1103 3 0.22153549991820753 1103 4 0.3092834307437329 1103 5 0.1870414186160403 1103 6 0.2852859740303231 1103 7 0.27195436624386105 1103 8 0.19792397174423115 1103 9 0.3998308363407759 1103 10-11 0.3716731696203581 1103 12-13 0.5085778303631727 1103 14-15 0.5968019172773538 1103 16-17 0.6477858033955854 1103 18-19 0.6998749322873934 1103 20-21 0.6174931153782452 1103 22-23 0.654254951976732 1103 24-25 0.46502221607763516 1103 26-27 0.5257458705425719 1103 28-29 0.6120673171727589 1103 30-31 0.5638327110966372 1103 32-33 0.4970812289317692 1103 34-35 0.6194047359518606 1103 36-37 0.6335435166153189 1103 38-39 0.6164395582182465 1103 40-41 0.7803274082879028 1103 42-43 0.776058811545461 1103 44-45 0.8375582140487055 1103 46-47 0.8765860038002558 1103 48-49 0.817583161095456 1103 50-51 0.8205057168031331 1103 52-53 0.8732765440494958 1103 54-55 0.887011979711545 1103 56-57 0.8743396488853747 1103 58-59 0.8441016707143731 1103 60-61 0.7418907987771988 1103 62-63 0.86713990349525 1103 64-65 0.7503364700904953 1103 66-67 0.8646435852688157 1103 68-69 0.8420014442565673 1103 70-71 0.6441542063668919 1103 72-73 0.8058241180883137 1103 74-75 0.8197374219657938 1103 76-77 0.6499715169797469 1103 78-79 0.6910198856161323 1103 80-81 0.6106171187345737 1103 82-83 0.7406999952047926 1103 84-85 0.7374237750052082 1103 86-87 0.6948118836885122 1103 88-89 0.6943197832121264 1103 90-91 0.6614592352458288 1103 92-93 0.7809497938586141 1103 94-95 0.7909578501485726 1103 96-97 0.7237900203196936 1103 98-99 0.7141312934512705 1103 100-101 0.6965450671845552 1104 1 0.14508025996200402 1104 2 0.19059018605640432 1104 3 0.2008853331734528 1104 4 0.23607473432735304 1104 5 0.11644777816172791 1104 6 0.14844853937746905 1104 7 0.14939762024451397 1104 8 0.12878392018934193 1104 9 0.16018952607488757 1104 10-11 0.2053743311914289 1104 12-13 0.22008574066369846 1104 14-15 0.1967032302785512 1104 16-17 0.2548160202599732 1104 18-19 0.2256842280800626 1104 20-21 0.2446800639933997 1104 22-23 0.4057218014134705 1104 24-25 0.2795974576664264 1104 26-27 0.2609675217238774 1104 28-29 0.2993224847521532 1104 30-31 0.2632934882473208 1104 32-33 0.2876913644544601 1104 34-35 0.20872810533478514 1104 36-37 0.3417038562722823 1104 38-39 0.4076483029965843 1104 40-41 0.2574307505153257 1104 42-43 0.28888896642333606 1104 44-45 0.3796736155647764 1104 46-47 0.3915270542116147 1104 48-49 0.420272112051741 1104 50-51 0.5353825836137744 1104 52-53 0.4507984902387605 1104 54-55 0.4996404834185171 1104 56-57 0.4300847376763741 1104 58-59 0.4943753448036503 1104 60-61 0.4831359840321028 1104 62-63 0.33686980195074057 1104 64-65 0.0869196534410861 1104 66-67 0.20402526201436189 1104 68-69 0.37215493107585473 1104 70-71 0.38008328121300394 1104 72-73 0.31899501677313324 1104 74-75 0.22743543688935475 1104 76-77 0.1937300550757115 1104 78-79 0.2898720561060806 1104 80-81 0.2570098755690786 1104 82-83 0.1591307900205976 1104 84-85 0.2822022044271719 1104 86-87 0.21029083089545608 1104 88-89 0.08421643682452995 1104 90-91 0.0577790591250924 1104 92-93 0.02963726067476813 1104 94-95 -0.02774193856686935 1104 96-97 0.08267944251907977 1104 98-99 0.14790056478214453 1104 100-101 0.4391195563028525 1105 1 0.18372097531819875 1105 2 0.21383810659978053 1105 3 0.2105208872906914 1105 4 0.2279924318933908 1105 5 0.22124609944275875 1105 6 0.1805440229402251 1105 7 0.23594924740246626 1105 8 0.22966000807551978 1105 9 0.24836619224070944 1105 10-11 0.24800945252998474 1105 12-13 0.31501747849484474 1105 14-15 0.4436071932214034 1105 16-17 0.2916928735351547 1105 18-19 0.2910592365879481 1105 20-21 0.3218533642454986 1105 22-23 0.4160797877212232 1105 24-25 0.48148717230218097 1105 26-27 0.33609019585950506 1105 28-29 0.43247583609010576 1105 30-31 0.4363276520791004 1105 32-33 0.5583117276546616 1105 34-35 0.5381579547355102 1105 36-37 0.5823573135575373 1105 38-39 0.7901770147159013 1105 40-41 0.6326699024745892 1105 42-43 0.6835806212431663 1105 44-45 0.7234568204129275 1105 46-47 0.8235580062348262 1105 48-49 0.8701183921455922 1105 50-51 1.0167425360466424 1105 52-53 1.0857431857462103 1105 54-55 1.0914233389616115 1105 56-57 1.1289825004545833 1105 58-59 1.1039794999926897 1105 60-61 1.136153170611081 1105 62-63 1.0135305740946876 1105 64-65 0.9606408694849762 1105 66-67 1.001820081255417 1105 68-69 0.9155742550935315 1105 70-71 0.8708183500273776 1105 72-73 0.8287154571543347 1105 74-75 0.7308699916345738 1105 76-77 0.7505646835345701 1105 78-79 0.6661195053444793 1105 80-81 0.7875684169122863 1105 82-83 0.8251554160266181 1105 84-85 0.7653265326249468 1105 86-87 0.6536481275825778 1105 88-89 0.6096280581521718 1105 90-91 0.8297784709570308 1105 92-93 0.8971755847040015 1105 94-95 0.9686311962790057 1105 96-97 0.7819052566759517 1105 98-99 0.8843422635861593 1105 100-101 1.016087960890566 1106 1 0.11944464345113914 1106 2 0.14778391549518588 1106 3 0.15220639152660098 1106 4 0.1624740932651747 1106 5 -0.010215186806838972 1106 6 0.10027383110249133 1106 7 0.05885216773482682 1106 8 0.14401576320748433 1106 9 0.04643865069623132 1106 10-11 0.16283181934328184 1106 12-13 0.11728435084740596 1106 14-15 0.13702588110833602 1106 16-17 0.18071782567754013 1106 18-19 0.22465196834097156 1106 20-21 0.1960871333885379 1106 22-23 0.14578943459317628 1106 24-25 0.24078083982417553 1106 26-27 -0.0369041121398439 1106 28-29 0.06851770640616905 1106 30-31 0.013068727144265324 1106 32-33 0.11625303589838865 1106 34-35 0.09625225458888309 1106 36-37 0.2708307576761442 1106 38-39 0.12466618565920129 1106 40-41 0.1937124091950153 1106 42-43 0.3042501440926273 1106 44-45 0.23873663468467754 1106 46-47 0.1583385230969938 1106 48-49 0.3768495138464729 1106 50-51 0.28348035272145466 1106 52-53 0.33027558127406564 1106 54-55 0.31183715453672534 1106 56-57 0.32244306374881404 1106 58-59 0.28010339010346996 1106 60-61 0.468949633221051 1106 62-63 0.24255557714942455 1106 64-65 0.2694285955358069 1106 66-67 0.12459037491785097 1106 68-69 0.34392416039655416 1106 70-71 0.4245537890954836 1106 72-73 0.37332714866623107 1106 74-75 0.28050355000049265 1106 76-77 0.1581422230425531 1106 78-79 0.29155544233535835 1106 80-81 0.24974486893248837 1106 82-83 0.32666713095819233 1106 84-85 0.44203107807949493 1106 86-87 0.49325551700095716 1106 88-89 0.5932858304314159 1106 90-91 0.6284612411997834 1106 92-93 0.6454760032035125 1106 94-95 0.6677388300766616 1106 96-97 0.7652076607570848 1106 98-99 0.8308520947608109 1106 100-101 1.2001116656803958 1107 1 0.17543601210966386 1107 2 0.12194693843769144 1107 3 0.12126703810993433 1107 4 0.1434677288714994 1107 5 0.10654283868660741 1107 6 0.176584650073508 1107 7 0.015149150850795934 1107 8 0.21518970337201893 1107 9 0.14904469403030873 1107 10-11 0.09765096673175577 1107 12-13 0.20673150953973618 1107 14-15 0.19434497120033 1107 16-17 0.3164837725343048 1107 18-19 0.356593935473704 1107 20-21 0.2526385128885522 1107 22-23 0.24843421533199006 1107 24-25 0.21949986733844185 1107 26-27 0.2855078057278959 1107 28-29 0.16485352159062927 1107 30-31 0.2922591054206265 1107 32-33 0.4397963101881075 1107 34-35 0.42512347899977954 1107 36-37 0.5702591878920202 1107 38-39 0.5446857137952676 1107 40-41 0.6523679522062409 1107 42-43 0.7577128819477608 1107 44-45 0.6477169366520883 1107 46-47 0.7314143328208615 1107 48-49 0.7916269743069222 1107 50-51 0.9194036157910332 1107 52-53 0.988595043045855 1107 54-55 0.8044633463035638 1107 56-57 0.7734781122565124 1107 58-59 0.7494663379128568 1107 60-61 0.6434035977390877 1107 62-63 0.7277806202961727 1107 64-65 0.7147935342734115 1107 66-67 0.7917654440722188 1107 68-69 0.8457098800204506 1107 70-71 0.8644570028801155 1107 72-73 0.7370231753793455 1107 74-75 0.8375917103549142 1107 76-77 0.6631734770283941 1107 78-79 0.548439926148852 1107 80-81 0.607764059766648 1107 82-83 0.6200138509089257 1107 84-85 0.5761297595628676 1107 86-87 0.6257550661025455 1107 88-89 0.616168239613998 1107 90-91 0.7665824471761518 1107 92-93 0.8653386137109926 1107 94-95 1.0463153139060992 1107 96-97 0.787905226503554 1107 98-99 1.0811378921086323 1107 100-101 1.1418516054144625 1108 1 0.16436762281282924 1108 2 0.09979719139516519 1108 3 -0.029235325662568812 1108 4 0.029305988801390015 1108 5 0.11993997355629915 1108 6 0.14489666949855007 1108 7 0.053564483969040566 1108 8 0.1782789527218398 1108 9 0.13485139336130914 1108 10-11 0.12916326724489835 1108 12-13 0.24322551241994717 1108 14-15 -0.018843359522755065 1108 16-17 0.1090657349490769 1108 18-19 0.08891290986574774 1108 20-21 0.17236102686720756 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-2.869518591825937 2316 68-69 -3.2013159206658948 2316 70-71 -2.964754443912746 2316 72-73 -3.099265893054696 2316 74-75 -3.156861970056447 2316 76-77 -3.2048132445601745 2316 78-79 -3.1177438700797886 2316 80-81 -2.9566694509464284 2316 82-83 -2.8642664098656994 2316 84-85 -3.085298276884348 2316 86-87 -3.1537019459918945 2316 88-89 -3.2203332483382248 2316 90-91 -3.4306073826434194 2316 92-93 -3.747455936632594 2316 94-95 -3.781083190562139 2316 96-97 -3.755818129519575 2316 98-99 -4.088596367546277 2316 100-101 -4.323607024574791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8783.0 3 2830.0 4 2743.0 5 3152.0 6 3625.0 7 4532.0 8 5617.0 9 7131.0 10 8843.0 11 11906.0 12 15574.0 13 20621.0 14 21711.0 15 22291.0 16 28288.0 17 36305.0 18 30457.0 19 21209.0 20 21205.0 21 22371.0 22 24621.0 23 27175.0 24 30387.0 25 34647.0 26 40283.0 27 46387.0 28 54728.0 29 64594.0 30 75904.0 31 92150.0 32 112641.0 33 143095.0 34 190073.0 35 276273.0 36 372559.0 37 511467.0 38 414734.0 39 60899.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.65032932985228 18.105050747417987 13.734760254982149 20.509859667747577 2 14.240216810555296 22.531457013076317 39.4405403813493 23.787785795019087 3 22.555695808151178 27.25039781475459 29.206468471394626 20.987437905699608 4 11.939966601638268 16.087156409386814 38.7358256509317 33.237051338043216 5 12.343937169065917 40.27317009227457 33.03302612331073 14.34986661534879 6 28.590447673434355 36.14472352286716 18.353677270222427 16.91115153347606 7 26.831965020232083 35.24827497798437 20.629619101762366 17.290140900021182 8 25.18540555472917 31.694151638734635 20.386476829400415 22.733965977135785 9 26.821289986131763 18.891872016509502 21.694502417448128 32.5923355799106 10-11 24.558089242067204 26.056610211589405 26.684538466375248 22.700762079968147 12-13 29.31154460425176 23.968356792906338 26.305697724669052 20.41440087817285 14-15 25.00711094838714 24.82192147318297 28.40037016993294 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20.914771455745775 26.937783232628398 31.62103474320242 20.526410568423408 42-43 20.267103374364456 28.14118591044041 29.660912609025353 21.93079810616978 44-45 21.034836427568727 27.496770388529345 28.796905449779146 22.671487734122785 46-47 21.65645289872436 27.59205771248211 28.21910342485089 22.53238596394264 48-49 22.19355865729265 27.07003049063152 28.678022079905396 22.05838877217043 50-51 21.660596903659997 27.830363344450205 29.717304308082877 20.791735443806928 52-53 21.18169900981647 28.571148690559863 28.972060542430118 21.27509175719355 54-55 19.78631241555125 27.381325730577334 29.839126499306918 22.993235354564494 56-57 21.805317657880263 28.08466213575425 27.846540042441237 22.26348016392425 58-59 22.325322395658496 26.9560360020141 28.28006112230055 22.438580480026854 60-61 21.423944735764906 26.699907610962104 30.07864428961988 21.79750336365311 62-63 22.531565345222518 26.558837859457046 29.414872463728575 21.494724331591858 64-65 21.475403669877245 27.938145502226646 29.558423336619434 21.02802749127667 66-67 21.05081485755454 27.379521745192847 29.018736658941442 22.550926738311173 68-69 20.40844428705005 28.5698938427686 28.87843429281703 22.14322757736432 70-71 21.461948021795425 28.305658059270637 27.65838435093555 22.57400956799838 72-73 21.406308060828678 26.939804632200065 28.304223171917087 23.34966413505417 74-75 20.976806901915563 28.84581003358383 28.05719011930848 22.120192945192134 76-77 21.881438628869823 26.799265545932766 28.72236265016325 22.59693317503416 78-79 21.343870407625303 27.071275631223386 29.533656649801404 22.051197311349913 80-81 21.54031512764847 28.550554459237215 28.449481388478194 21.459649024636125 82-83 21.17325572955971 27.771511918201234 28.395513415245187 22.659718936993862 84-85 21.09448001338929 27.476778168881687 28.171024044583397 23.257717773145625 86-87 21.880574579976525 27.478001643765943 28.40540536286072 22.236018413396817 88-89 22.2401403315066 26.62079444103633 28.23863702954249 22.900428197914586 90-91 21.517915838225843 26.621805203897026 29.035143850447177 22.825135107429954 92-93 21.604693147109554 28.191542958704925 28.473572620626197 21.730191273559328 94-95 21.234643147921567 28.08257545513803 27.956984544568066 22.725796852372337 96-97 20.631781919127633 27.22260107296595 28.198735575437734 23.94688143246868 98-99 21.906171039198494 27.49041187143616 27.159821735330564 23.44359535403478 100-101 22.447272970874526 26.533011009354357 28.122487309848882 22.89722870992224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10117.0 1 6173.5 2 3373.0 3 4236.5 4 3171.0 5 2978.0 6 5387.5 7 10301.0 8 14635.0 9 14704.0 10 11037.0 11 7950.0 12 7379.5 13 7471.5 14 7600.0 15 7441.0 16 7547.0 17 7655.5 18 7511.5 19 7703.5 20 8080.5 21 8751.5 22 9662.0 23 10968.0 24 12216.0 25 13849.0 26 16505.0 27 19341.0 28 21495.0 29 24291.5 30 27979.0 31 31390.5 32 36499.5 33 41945.0 34 47579.0 35 52300.5 36 57267.0 37 63096.0 38 65512.5 39 65894.0 40 66373.5 41 67533.5 42 68340.0 43 71948.0 44 78699.0 45 83542.0 46 93533.5 47 130412.0 48 244843.0 49 246474.5 50 145950.0 51 109468.5 52 81804.0 53 72939.5 54 69446.5 55 64609.0 56 59201.0 57 53737.5 58 49000.0 59 43853.5 60 38109.5 61 32976.0 62 27789.0 63 21285.0 64 15704.0 65 12379.0 66 9242.0 67 7271.0 68 5423.0 69 3567.5 70 2792.5 71 2305.5 72 1795.0 73 1443.5 74 1156.0 75 950.5 76 693.0 77 394.5 78 248.5 79 178.5 80 122.5 81 86.0 82 58.5 83 47.0 84 37.0 85 27.5 86 25.5 87 14.5 88 9.0 89 11.0 90 9.5 91 9.5 92 10.0 93 5.5 94 2.0 95 3.5 96 4.0 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15662454262708594 2 0.0906388804931758 3 0.08391844102518389 4 0.09864769436694852 5 0.11978399880773136 6 0.036144125221635216 7 0.03997442750909174 8 0.043073490268942936 9 0.04342169956780262 10-11 0.04197663097753493 12-13 0.04632924721328098 14-15 0.04396142398103513 16-17 0.04269046004019728 18-19 0.04693861348628543 20-21 0.048853764630013695 22-23 0.061946434267137813 24-25 0.07808593526928417 26-27 0.07963546664920977 28-29 0.06985078535125265 30-31 0.05813354244462428 32-33 0.047652442548947786 34-35 0.29091145873232316 36-37 0.4645112046788187 38-39 0.44222580955179897 40-41 0.4177118749120771 42-43 0.4480757257726416 44-45 0.4573032721924232 46-47 0.4566068535947039 48-49 0.4729030487813371 50-51 0.461986687262086 52-53 0.4692816720731964 54-55 0.4604545663471034 56-57 0.4383432758695134 58-59 0.4177118749120771 60-61 0.38675606824345127 62-63 0.44455881185415885 64-65 0.4818694382269739 66-67 0.4834015591419566 68-69 0.4940393532221199 70-71 0.49029610325937834 72-73 0.4819913114815748 74-75 0.47408696039746 76-77 0.4566416745245898 78-79 0.4320755084900391 80-81 0.43590581077749563 82-83 0.4480931362375846 84-85 0.44690922462146165 86-87 0.4581215640447435 88-89 0.4687419476599639 90-91 0.47199770460430196 92-93 0.47446999062620565 94-95 0.47868332314240786 96-97 0.4797279510389869 98-99 0.474052139467574 100-101 0.4603849244873314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2871836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.31013860513192 #Duplication Level Percentage of deduplicated Percentage of total 1 68.55757157939206 22.836622117408023 2 13.660846473318736 9.100893789793492 3 6.001447318503204 5.99727126032217 4 3.2959648366168737 4.391561821813961 5 2.0267410114190874 3.3755512003537533 6 1.3423421158781783 2.68281611612449 7 0.9465233778686284 2.2070177436861167 8 0.6851878343738883 1.8258961386835515 9 0.5332085595334528 1.5985125921151841 >10 2.64038220825721 15.596402594400265 >50 0.18353952167572082 4.242007137645125 >100 0.10945018342197543 6.71224724329745 >500 0.0071378663898951835 1.7165462660987254 >1k 0.006717991896371763 5.230653152655169 >5k 0.001574529350712132 3.809632705737746 >10k+ 0.0013645921039505144 8.676368119864913 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33465 1.1652824186339332 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32529 1.1326900282606667 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 28692 0.9990821202882059 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24047 0.8373389009678825 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 19138 0.6664029561576637 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18922 0.6588816353022944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17384 0.605327045137675 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13311 0.4635013977121257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13243 0.46113357447987974 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13039 0.4540301047831422 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12221 0.42554658413642005 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 11695 0.40723077501640065 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10457 0.3641224638175718 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9492 0.33052026647761223 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8927 0.31084644109204007 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8881 0.3092446783172855 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 8839 0.3077821992620749 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8286 0.28852622503513436 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 8078 0.28128347161885286 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8047 0.2802040227923879 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7001 0.24378133013166492 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6983 0.24315455339371747 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6257 0.2178745582965044 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6220 0.21658618389072357 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6107 0.21265141881360913 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5566 0.19381329574530023 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5141 0.17901440054376364 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5130 0.178631370315018 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4953 0.17246806572520157 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4808 0.16741903089173615 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4804 0.16727974717219227 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4789 0.16675743322390274 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4410 0.1535603007971207 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4358 0.15174961244305038 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.14711842876821657 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3988 0.13886586838524206 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3972 0.13830873350706654 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3814 0.13280702658508356 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3688 0.12841958941945153 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3663 0.12754906617230233 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3572 0.1243803615526792 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3480 0.12117683600317011 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3251 0.11320284305928333 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3162 0.11010378029943214 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3133 0.10909397333273906 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3106 0.10815380822581791 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2968 0.10334851990155428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.482092988596842E-5 0.0 10-11 0.0 0.0 0.0 3.482092988596842E-5 0.0 12-13 0.0 0.0 0.0 6.964185977193684E-5 0.0 14-15 0.0 0.0 0.0 1.2187325460088946E-4 0.0 16-17 0.0 0.0 0.0 2.7856743908774735E-4 0.0 18-19 0.0 0.0 0.0 5.049034833465421E-4 0.0 20-21 0.0 0.0 0.0 0.0010446278965790525 0.0 22-23 0.0 0.0 0.0 0.002420054627074805 0.0 24-25 0.0 0.0 0.0 0.0042307429811451625 0.0 26-27 0.0 0.0 0.0 0.006198125519702379 0.0 28-29 0.0 0.0 0.0 0.012901154522751299 0.0 30-31 0.0 0.0 0.0 0.030033052026647758 0.0 32-33 0.0 0.0 0.0 0.0641749737798398 0.0 34-35 0.0 0.0 0.0 0.12190807553077544 0.0 36-37 0.0 0.0 0.0 0.2056175909766435 0.0 38-39 0.0 0.0 0.0 0.31781062706923374 0.0 40-41 0.0 0.0 0.0 0.4429744595443472 0.0 42-43 0.0 0.0 0.0 0.5631414885808242 0.0 44-45 0.0 0.0 0.0 0.7038876871799087 0.0 46-47 0.0 0.0 0.0 0.8869064946605586 0.0 48-49 0.0 0.0 0.0 1.088014775216969 0.0 50-51 0.0 0.0 0.0 1.2995867452041132 0.0 52-53 0.0 0.0 0.0 1.497491500211015 0.0 54-55 0.0 0.0 0.0 1.7050764737262156 0.0 56-57 0.0 0.0 0.0 1.9674347699520447 0.0 58-59 0.0 0.0 0.0 2.279029861036633 0.0 60-61 0.0 0.0 0.0 2.653720477074596 0.0 62-63 0.0 0.0 0.0 2.992388841145525 0.0 64-65 0.0 0.0 0.0 3.296462611374744 0.0 66-67 0.0 0.0 0.0 3.6461517997545823 0.0 68-69 0.0 0.0 0.0 4.051328139907711 0.0 70-71 1.741046494298421E-5 0.0 0.0 4.512426893457704 0.0 72-73 3.482092988596842E-5 0.0 0.0 4.975562671406028 0.0 74-75 3.482092988596842E-5 0.0 0.0 5.433179331967424 0.0 76-77 3.482092988596842E-5 0.0 0.0 5.876832799644548 0.0 78-79 3.482092988596842E-5 0.0 0.0 6.4103242664274696 0.0 80-81 3.482092988596842E-5 0.0 0.0 6.999093959404368 0.0 82-83 3.482092988596842E-5 0.0 0.0 7.5779570978287065 0.0 84-85 3.482092988596842E-5 0.0 0.0 8.239937795890853 0.0 86-87 3.482092988596842E-5 0.0 0.0 8.92183606584777 0.0 88-89 3.482092988596842E-5 0.0 0.0 9.562697869934077 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 6575 0.0 22.553919 4 GAGTACT 16370 0.0 22.083742 12-13 TACCTGG 2065 0.0 21.107714 2 AGTACTT 16660 0.0 20.931969 12-13 ATGGGAG 4630 0.0 20.874815 5 GTATAGG 800 0.0 20.737476 1 TATCACG 160 5.0927913E-5 20.727718 2 ATGGGAT 5305 0.0 20.719345 5 GTACTTT 17750 0.0 20.40718 14-15 GTACATG 33820 0.0 19.831736 1 TACATGG 33920 0.0 19.66619 2 ACATGGG 33390 0.0 19.041868 3 GCTTATA 3585 0.0 19.039309 1 TATAACG 125 0.0061238417 18.951056 2 ATGGGGA 3520 0.0 18.708784 5 ACTTTTT 19890 0.0 18.70086 16-17 CTTACTA 920 0.0 18.527452 9 TATACTC 3465 0.0 18.45882 4 GCTTATG 705 0.0 18.153173 1 TACTTTT 19500 0.0 17.883728 14-15 >>END_MODULE