##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938747_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2871836 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9496148108736 31.0 31.0 34.0 30.0 34.0 2 32.13923288098624 33.0 31.0 34.0 30.0 34.0 3 32.2291133616265 34.0 31.0 34.0 30.0 34.0 4 35.83957475287586 37.0 35.0 37.0 35.0 37.0 5 35.76531981631263 37.0 35.0 37.0 35.0 37.0 6 35.8352875999883 37.0 35.0 37.0 35.0 37.0 7 35.77771432630554 37.0 35.0 37.0 33.0 37.0 8 35.7701230153811 37.0 35.0 37.0 33.0 37.0 9 37.37838476848957 39.0 37.0 39.0 34.0 39.0 10-11 37.295336850711536 39.0 37.0 39.0 33.5 39.0 12-13 37.22888789610549 39.0 37.0 39.0 33.0 39.0 14-15 38.301888756878874 40.0 38.0 41.0 33.0 41.0 16-17 38.257560668506144 40.0 37.5 41.0 33.0 41.0 18-19 38.37501706225565 40.0 38.0 41.0 33.5 41.0 20-21 38.470754075093424 40.0 38.0 41.0 34.0 41.0 22-23 38.43499472114703 40.0 38.0 41.0 34.0 41.0 24-25 38.33564451451963 40.0 38.0 41.0 33.5 41.0 26-27 38.09143680210151 40.0 37.5 41.0 33.0 41.0 28-29 37.910378587078085 40.0 37.0 41.0 33.0 41.0 30-31 37.6902100259207 40.0 37.0 41.0 33.0 41.0 32-33 37.42566393067014 40.0 36.0 41.0 32.0 41.0 34-35 37.34266911481018 40.0 36.0 41.0 32.0 41.0 36-37 37.19597271571217 40.0 35.5 41.0 31.5 41.0 38-39 37.030876589053136 39.0 35.0 41.0 31.0 41.0 40-41 36.86208004217511 39.0 35.0 41.0 31.0 41.0 42-43 36.68056671759808 39.0 35.0 41.0 30.5 41.0 44-45 36.38433966981401 39.0 35.0 41.0 30.0 41.0 46-47 35.98176166744898 39.0 35.0 41.0 29.5 41.0 48-49 35.62794428372651 39.0 35.0 41.0 27.5 41.0 50-51 35.366526674921545 39.0 35.0 40.5 27.0 41.0 52-53 35.11308863040926 38.0 35.0 40.0 26.0 41.0 54-55 34.905643114718245 38.0 34.0 40.0 26.0 41.0 56-57 34.67582236590112 38.0 34.0 40.0 25.5 41.0 58-59 34.4022527748799 38.0 34.0 40.0 25.0 41.0 60-61 33.97675128384768 37.5 33.5 40.0 23.0 41.0 62-63 33.57582623102434 37.0 33.0 39.5 22.5 41.0 64-65 33.15675929962575 36.0 32.5 39.0 22.0 40.5 66-67 32.72234330233342 36.0 32.0 39.0 21.5 40.0 68-69 32.29327475524369 35.0 32.0 38.0 20.0 40.0 70-71 31.753501244500036 35.0 31.0 37.5 18.5 39.5 72-73 31.357872803321637 35.0 31.0 37.0 16.5 39.0 74-75 30.940098076631116 35.0 31.0 36.5 12.5 39.0 76-77 28.87892292596095 32.5 28.0 34.5 7.5 36.5 78-79 29.919775015007822 34.0 29.5 35.0 7.0 37.0 80-81 29.98585312671058 34.0 30.0 35.0 6.0 37.0 82-83 29.69560552900653 34.0 30.0 35.0 2.0 36.5 84-85 29.328733778669815 34.0 29.5 35.0 2.0 36.0 86-87 28.805690331899175 34.0 29.0 35.0 2.0 36.0 88-89 28.231511827277046 34.0 28.5 35.0 2.0 35.0 90-91 27.75499645522934 33.0 27.0 35.0 2.0 35.0 92-93 27.50663808796881 33.0 27.0 35.0 2.0 35.0 94-95 27.151091670972853 33.0 26.0 35.0 2.0 35.0 96-97 26.565953975087716 33.0 25.0 35.0 2.0 35.0 98-99 25.81638349125786 33.0 24.5 35.0 2.0 35.0 100-101 22.655437148917976 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.037280900438450004 1101 2 0.08204116226740865 1101 3 0.11492226128233085 1101 4 0.11764116885971987 1101 5 0.046992471858075646 1101 6 0.171441954882944 1101 7 0.15166351779969744 1101 8 0.0399765179538889 1101 9 0.13651776354597445 1101 10-11 0.167139822829931 1101 12-13 0.2131607455967668 1101 14-15 0.23250178352517992 1101 16-17 0.2396993302732966 1101 18-19 0.26464897794722475 1101 20-21 0.26109203890888466 1101 22-23 0.2450945678424432 1101 24-25 0.21790455448905988 1101 26-27 0.26038153783668605 1101 28-29 0.3659283140676095 1101 30-31 0.29470490602447796 1101 32-33 0.34703552958252004 1101 34-35 0.331208052866522 1101 36-37 0.32552821406068944 1101 38-39 0.37146607723583003 1101 40-41 0.478575732721211 1101 42-43 0.4036903289213356 1101 44-45 0.47229206965311477 1101 46-47 0.560088991906035 1101 48-49 0.5505919939497872 1101 50-51 0.630855489407125 1101 52-53 0.6427687117893655 1101 54-55 0.5993565016815978 1101 56-57 0.567602819051551 1101 58-59 0.6170342629149062 1101 60-61 0.601687364216204 1101 62-63 0.5320353147124592 1101 64-65 0.5041636233220217 1101 66-67 0.43269493779415313 1101 68-69 0.42020124958980176 1101 70-71 0.3006625005289756 1101 72-73 0.38788909421893436 1101 74-75 0.5058480793023747 1101 76-77 0.42310006323908667 1101 78-79 0.5179145962658218 1101 80-81 0.5701246980333252 1101 82-83 0.6345126152650629 1101 84-85 0.6318878929034177 1101 86-87 0.650129042455319 1101 88-89 0.7005284176219284 1101 90-91 0.6872498144968766 1101 92-93 0.7245472589677426 1101 94-95 0.6805676498026187 1101 96-97 0.6224126301460622 1101 98-99 0.6958326009406335 1101 100-101 1.4777993911636749 1102 1 0.03617404826860593 1102 2 0.020871099375668223 1102 3 0.09965493149673676 1102 4 -0.01965209380236388 1102 5 0.08966662032086958 1102 6 0.0499473854760808 1102 7 0.03803068224858208 1102 8 0.05015695293296574 1102 9 0.11503754812979139 1102 10-11 0.07638897621028917 1102 12-13 0.006123967482885462 1102 14-15 0.02002098863307822 1102 16-17 0.08462333386710696 1102 18-19 0.11828731586142993 1102 20-21 0.0972314002094592 1102 22-23 0.10009440835365524 1102 24-25 0.08703018380521854 1102 26-27 0.05443832116053926 1102 28-29 0.11640571716488779 1102 30-31 0.06625051184263242 1102 32-33 0.14913931956768778 1102 34-35 0.09477416894245749 1102 36-37 0.163749435638465 1102 38-39 0.2306007974127624 1102 40-41 0.1691573883211248 1102 42-43 0.1763612610269618 1102 44-45 0.20340660386612086 1102 46-47 0.12061565045649303 1102 48-49 0.17660155796060906 1102 50-51 0.21030722012626768 1102 52-53 0.19285922167500757 1102 54-55 0.22333138751421444 1102 56-57 0.17822424052193497 1102 58-59 0.08683599177334145 1102 60-61 0.09351608920969312 1102 62-63 -0.02731125973424753 1102 64-65 -0.07694838565187467 1102 66-67 0.017059986358482604 1102 68-69 0.15119364197474994 1102 70-71 0.19663766812510985 1102 72-73 0.17664619811524318 1102 74-75 0.18282587314716992 1102 76-77 0.07729289049678201 1102 78-79 0.24395207081407122 1102 80-81 0.2847385225212342 1102 82-83 0.46047380101101965 1102 84-85 0.5427917424310671 1102 86-87 0.3537300334123046 1102 88-89 0.4805330427967007 1102 90-91 0.5481201713796153 1102 92-93 0.5645326923253542 1102 94-95 0.6698328583873696 1102 96-97 0.6534878237920303 1102 98-99 0.839166102623242 1102 100-101 1.2723383622483553 1103 1 0.11277241891049528 1103 2 0.11603122777501795 1103 3 0.047573739035428275 1103 4 0.08582182905208668 1103 5 0.061429356964481485 1103 6 0.07268477066354251 1103 7 0.1047077663543945 1103 8 0.07874381848444045 1103 9 0.06422445817608491 1103 10-11 0.06690840032012346 1103 12-13 0.10320717280530545 1103 14-15 0.2045611558064877 1103 16-17 0.032459508159689676 1103 18-19 0.15158109823936172 1103 20-21 0.15585386162079118 1103 22-23 0.06838946201229135 1103 24-25 0.12477185956507242 1103 26-27 0.10555837243553157 1103 28-29 0.12826014492965498 1103 30-31 0.07933543339401439 1103 32-33 0.11882774985382838 1103 34-35 0.12095698970247071 1103 36-37 0.05180654792432904 1103 38-39 0.3840875496083669 1103 40-41 0.29292398118288077 1103 42-43 0.2429390263273561 1103 44-45 0.3598986106886528 1103 46-47 0.39315439803311136 1103 48-49 0.47018185522841094 1103 50-51 0.48741224910087055 1103 52-53 0.48750262743602946 1103 54-55 0.2966284010639484 1103 56-57 0.23644492425906805 1103 58-59 0.053199758154320875 1103 60-61 0.1065792930489664 1103 62-63 0.19042284131138842 1103 64-65 0.2229268699285143 1103 66-67 0.08493233918125753 1103 68-69 0.2977900527255528 1103 70-71 0.29166881450248994 1103 72-73 0.3358847009703254 1103 74-75 0.2823846328009587 1103 76-77 0.1971153349577044 1103 78-79 0.19235661352380973 1103 80-81 0.3029247227691343 1103 82-83 0.2119596103400987 1103 84-85 0.018573580067332784 1103 86-87 0.25198758429002055 1103 88-89 0.4042580541852594 1103 90-91 0.4422783716698895 1103 92-93 0.3930522859392873 1103 94-95 0.34458151559712036 1103 96-97 0.5709213199199183 1103 98-99 0.7845881678187538 1103 100-101 1.2384205929222425 1104 1 -0.06044542987673651 1104 2 0.00591962605291485 1104 3 0.03958855547578821 1104 4 9.720709398877148E-4 1104 5 0.01906207024202189 1104 6 -0.036292405091622015 1104 7 0.008580311269916763 1104 8 0.04079775090978188 1104 9 0.023804910376007626 1104 10-11 -0.0234084043167897 1104 12-13 -0.039040691078135126 1104 14-15 0.003813261501804277 1104 16-17 0.12390274343958652 1104 18-19 0.09722947091142231 1104 20-21 -0.014943029069215186 1104 22-23 0.06648592488097194 1104 24-25 0.05361516629586305 1104 26-27 -0.023071309639185245 1104 28-29 0.08787004332273085 1104 30-31 -0.014597207756601449 1104 32-33 -0.08640513118250936 1104 34-35 -0.08917305976713408 1104 36-37 -0.1225893435971912 1104 38-39 0.05510330416674236 1104 40-41 0.05544673860501348 1104 42-43 0.013995719544560359 1104 44-45 -0.14358169533334575 1104 46-47 -0.02019507588757108 1104 48-49 -0.06173209792496692 1104 50-51 -0.05258523659725256 1104 52-53 -0.1546551427968197 1104 54-55 -0.1976459719336532 1104 56-57 -0.22799739994390933 1104 58-59 -0.151301648242665 1104 60-61 -0.12890392873118373 1104 62-63 -0.2412279941804698 1104 64-65 -0.019447346074713323 1104 66-67 -0.10750633343375426 1104 68-69 -0.09041991254224513 1104 70-71 -0.11354018456615478 1104 72-73 -0.02941957115877969 1104 74-75 -0.05323241640187604 1104 76-77 -0.04820506042471706 1104 78-79 -0.07947705617648992 1104 80-81 -0.061213207099786615 1104 82-83 -0.04230657752583156 1104 84-85 -0.046390182264318014 1104 86-87 0.24238975038579014 1104 88-89 0.3164922129133707 1104 90-91 0.23297327062719475 1104 92-93 0.2948802380079023 1104 94-95 0.2562931831538897 1104 96-97 0.3692434329319383 1104 98-99 0.2921230131306878 1104 100-101 0.8073315101926077 1105 1 -0.0450702287373872 1105 2 -0.0011928411836805708 1105 3 -0.03414409003480756 1105 4 -0.05786207208768701 1105 5 0.07239298926103999 1105 6 -0.05643228121675037 1105 7 -0.04238817550722018 1105 8 0.01779293127061976 1105 9 -0.03948852256063162 1105 10-11 -0.031012980726636385 1105 12-13 -0.00814427415173924 1105 14-15 0.003211737658531888 1105 16-17 -0.05373184495192618 1105 18-19 -0.08126727169057091 1105 20-21 0.012826722397676349 1105 22-23 0.030830483491655514 1105 24-25 -0.06720810204361527 1105 26-27 -0.13899037360439337 1105 28-29 -0.06122555196626678 1105 30-31 -0.10323644627158757 1105 32-33 0.12035494890081821 1105 34-35 0.09802221331364791 1105 36-37 0.0426757365567596 1105 38-39 -0.050056136893807945 1105 40-41 -0.11518319195776883 1105 42-43 -0.01316794913446273 1105 44-45 0.0323178614858719 1105 46-47 0.07621241156891045 1105 48-49 0.16428894513587267 1105 50-51 0.15112426745378826 1105 52-53 0.08781579232618952 1105 54-55 0.09329842306871683 1105 56-57 0.006221781269651672 1105 58-59 0.12926267204438346 1105 60-61 -0.016584504673573974 1105 62-63 -0.004355186165973635 1105 64-65 0.08671223753312063 1105 66-67 0.11781738373702666 1105 68-69 0.15822736512573243 1105 70-71 -0.005954357618552564 1105 72-73 -0.24792670301758335 1105 74-75 -0.13336594853087647 1105 76-77 -0.12746667834919734 1105 78-79 -0.061062580050592175 1105 80-81 -0.06069027689273199 1105 82-83 -0.12826400083603673 1105 84-85 0.10963173405914262 1105 86-87 0.16624998213427844 1105 88-89 0.25934815411710943 1105 90-91 0.42491228607495657 1105 92-93 0.3846043769131633 1105 94-95 0.21433028140492283 1105 96-97 0.2778233024305443 1105 98-99 0.53787041677964 1105 100-101 0.9555402302335985 1106 1 -0.05775376775661201 1106 2 -0.08424564747237895 1106 3 -0.049597515417666216 1106 4 -0.014055813941709516 1106 5 0.005982666273091297 1106 6 -0.13083915234740573 1106 7 -0.005605785070592617 1106 8 -0.022559433098884085 1106 9 -0.10946321912302892 1106 10-11 -0.10990726632454795 1106 12-13 -0.019340790728797685 1106 14-15 -0.01218237483739415 1106 16-17 -0.08756297378053546 1106 18-19 -0.1669225899945772 1106 20-21 -0.15217320123380063 1106 22-23 -0.1430428262183483 1106 24-25 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0.22693187947553284 1106 90-91 0.21939147819779592 1106 92-93 0.33562644232535277 1106 94-95 0.40292400594875133 1106 96-97 0.45591037273031887 1106 98-99 0.46190944618661334 1106 100-101 0.7517355503704515 1107 1 -0.049504942583624256 1107 2 -0.07488464774340997 1107 3 -0.1126931497246062 1107 4 -0.04152686177979348 1107 5 -0.05340227342111348 1107 6 -0.06350557122754452 1107 7 0.009325553813688714 1107 8 -0.019178816050640535 1107 9 -0.07359225777772593 1107 10-11 -0.03883944921093274 1107 12-13 -0.04116632891715 1107 14-15 -0.1032736934735965 1107 16-17 0.023772998346473173 1107 18-19 -0.01593969374206239 1107 20-21 -0.03499087037301507 1107 22-23 0.007812087129444478 1107 24-25 -0.07184427950257088 1107 26-27 0.031769555943057526 1107 28-29 -0.06502274819622755 1107 30-31 -0.11624276110295995 1107 32-33 -0.05032565238477815 1107 34-35 -0.08373241217775984 1107 36-37 -0.0076733203672390005 1107 38-39 -0.08197476193765141 1107 40-41 -0.06733460962043836 1107 42-43 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26-27 0.515127232470995 2101 28-29 0.5275038658093436 2101 30-31 0.5374383642596925 2101 32-33 0.5727698524827218 2101 34-35 0.5094851672450744 2101 36-37 0.5785137727396688 2101 38-39 0.6167905153740136 2101 40-41 0.7231041966640461 2101 42-43 0.6318048401391749 2101 44-45 0.6672091347001441 2101 46-47 0.6296247837404323 2101 48-49 0.7240070064006474 2101 50-51 0.7762641917437136 2101 52-53 0.7502839561634076 2101 54-55 0.8104725330108877 2101 56-57 0.6332803907744378 2101 58-59 0.6969949308830294 2101 60-61 0.7511628497316281 2101 62-63 0.7745663710771851 2101 64-65 0.7177405939742769 2101 66-67 0.6102730840552013 2101 68-69 0.6496581151818575 2101 70-71 0.688525309814505 2101 72-73 0.7541464214550402 2101 74-75 0.8411364783717445 2101 76-77 0.9237823830162135 2101 78-79 0.8661287930266575 2101 80-81 0.8862190026229726 2101 82-83 0.8212010742160238 2101 84-85 0.6888203667104662 2101 86-87 0.6664036472604238 2101 88-89 0.7039598109910123 2101 90-91 0.8086133319267717 2101 92-93 0.8110049745427759 2101 94-95 0.34358819883604497 2101 96-97 0.613732945788108 2101 98-99 0.9587026856819989 2101 100-101 1.3291206663793567 2102 1 0.2012152696505325 2102 2 0.12384541637622704 2102 3 0.17142686997321022 2102 4 0.12382361876080239 2102 5 0.0973933379214813 2102 6 0.1412276366515286 2102 7 0.11621200985335634 2102 8 0.172599690972568 2102 9 0.24389419377050103 2102 10-11 0.15975525136204993 2102 12-13 0.21397094100979785 2102 14-15 0.25723280689235395 2102 16-17 0.3370679567553623 2102 18-19 0.3275842027627647 2102 20-21 0.2078394833700159 2102 22-23 0.20293181562814055 2102 24-25 0.2630205684950866 2102 26-27 0.2911878996515753 2102 28-29 0.353049240644161 2102 30-31 0.22909756282907523 2102 32-33 0.08374610915402769 2102 34-35 0.2727324130897415 2102 36-37 0.3025168995358669 2102 38-39 0.4354408563852914 2102 40-41 0.3679936879242689 2102 42-43 0.5270197316142813 2102 44-45 0.6553742696747022 2102 46-47 0.4284543616331291 2102 48-49 0.522967737578945 2102 50-51 0.592238751062311 2102 52-53 0.5001801158678703 2102 54-55 0.4956844015937705 2102 56-57 0.4131506152350113 2102 58-59 0.397732547147875 2102 60-61 0.525017913381717 2102 62-63 0.4386429513533159 2102 64-65 0.5719327113816774 2102 66-67 0.4573984587015616 2102 68-69 0.4074108023598839 2102 70-71 0.5083485813988311 2102 72-73 0.5957506884444186 2102 74-75 0.6703657325322965 2102 76-77 0.5241461614837952 2102 78-79 0.5713719066514997 2102 80-81 0.5417145926892424 2102 82-83 0.5534003900288091 2102 84-85 0.43686369975602446 2102 86-87 0.30799934471959034 2102 88-89 0.48789273130609345 2102 90-91 0.5216698707075267 2102 92-93 0.4608185076773168 2102 94-95 0.35383610910580643 2102 96-97 0.6126951158325475 2102 98-99 0.7490139850086877 2102 100-101 1.1911009332852025 2103 1 0.26129574551560353 2103 2 0.18315367294543705 2103 3 0.23047908731024336 2103 4 0.143663303400011 2103 5 0.16395682657783084 2103 6 0.18769353707315162 2103 7 0.1463576464601033 2103 8 0.15256499392788214 2103 9 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74-75 0.5036862431888736 2103 76-77 0.4613505511397129 2103 78-79 0.5362287198181797 2103 80-81 0.44871523974761374 2103 82-83 0.5745826027099099 2103 84-85 0.4609664376108782 2103 86-87 0.650909581093515 2103 88-89 0.5602574350798868 2103 90-91 0.7002810989973263 2103 92-93 0.6723682510041975 2103 94-95 0.4988115504689681 2103 96-97 0.3727286843159554 2103 98-99 0.4841683083056978 2103 100-101 0.9453750483900016 2104 1 0.15648909580165338 2104 2 0.128053731627908 2104 3 0.1825355094183223 2104 4 0.09668472612388967 2104 5 0.15947498312720398 2104 6 0.12440871941141296 2104 7 0.19691271500750673 2104 8 0.17647822114978595 2104 9 0.18430410944616682 2104 10-11 0.16844568978292074 2104 12-13 0.29088391932274504 2104 14-15 0.3925458839098752 2104 16-17 0.30945590215667096 2104 18-19 0.2799454541713047 2104 20-21 0.2108868044284904 2104 22-23 0.269050562566413 2104 24-25 0.26565410835434733 2104 26-27 0.27668416358660863 2104 28-29 0.259082617859697 2104 30-31 0.3431737817149596 2104 32-33 0.21276525078899766 2104 34-35 0.2745030173634575 2104 36-37 0.3581119409686835 2104 38-39 0.40011568441203593 2104 40-41 0.46071933178313174 2104 42-43 0.5721089972499058 2104 44-45 0.3811908615228248 2104 46-47 0.4613341645157405 2104 48-49 0.3431660097628537 2104 50-51 0.24514932010870893 2104 52-53 0.36947710112418264 2104 54-55 0.3224747893252342 2104 56-57 0.39773787335108324 2104 58-59 0.41204543818265194 2104 60-61 0.37052150722459487 2104 62-63 0.33363764537524787 2104 64-65 0.4300222918508183 2104 66-67 0.2451646844797395 2104 68-69 0.2518884522188145 2104 70-71 0.32887548386243637 2104 72-73 0.2869216138726358 2104 74-75 0.11657308261866461 2104 76-77 0.24703540937204238 2104 78-79 0.4279833498167527 2104 80-81 0.4081620306858831 2104 82-83 0.38423091842642876 2104 84-85 0.4399203199513195 2104 86-87 0.4800751308613229 2104 88-89 0.6097564606610995 2104 90-91 0.5231201713796167 2104 92-93 0.4281065754868614 2104 94-95 0.5166378600177914 2104 96-97 0.4324384596562787 2104 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15 19270.0 16 25238.0 17 34416.0 18 31781.0 19 20116.0 20 18043.0 21 19270.0 22 20253.0 23 22373.0 24 25528.0 25 30335.0 26 36016.0 27 43673.0 28 53138.0 29 63780.0 30 77575.0 31 93638.0 32 114953.0 33 143602.0 34 191222.0 35 278432.0 36 397907.0 37 579106.0 38 441540.0 39 39259.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.29833647787971 18.96004177504808 14.34287136347018 20.398750383602035 2 14.750668213644511 23.323058837621645 38.34062947884211 23.585643469891735 3 22.642657867649824 27.110740306897746 29.5703863312529 20.67621549419953 4 12.43838436456678 16.865169180969943 37.9681499918519 32.728296462611375 5 12.234089968925803 40.49162278068803 33.10641694024311 14.167870310143057 6 28.661803807738323 36.77581171069657 17.722181907323396 16.840202574241705 7 26.672623367072497 35.264200323416794 20.44681520811077 17.616361101399942 8 25.308165229490818 32.29077147859418 19.949990180497775 22.451073111417223 9 26.418883250993442 18.983674555232263 21.409335352018708 33.18810684175559 10-11 24.644217148890117 26.37231025727096 26.40913339062537 22.574339203213555 12-13 29.267705398219118 24.146678292214457 26.184294646351674 20.401321663214752 14-15 24.80329601912092 25.174575218293015 28.23981798352826 21.7823107790578 16-17 20.439234186848466 28.92981369336204 29.158905776261406 21.47204634352809 18-19 20.07069350108676 29.38885046976949 30.79777591601743 19.742680113126315 20-21 21.945947112917853 26.78341081862105 29.239627409021164 22.03101465943993 22-23 20.744743084215113 26.797613094898175 28.980067106896072 23.47757671399063 24-25 22.233912382183384 26.97105614666018 29.63299436318787 21.162037107968562 26-27 22.739342948801653 25.032427047413304 31.616989300554245 20.611240703230795 28-29 20.520304780635108 27.119863390527872 31.784544799911973 20.57528702892505 30-31 20.733286998282633 27.74972178077021 30.499443561540424 21.017547659406734 32-33 20.505906326127256 27.673133145486023 30.19270599017493 21.62825453821179 34-35 21.886279097666964 26.173818398485444 30.176168307534425 21.76373419631317 36-37 22.59778637625335 25.774228488042045 31.152052547443503 20.475932588261106 38-39 22.617210190603682 25.74709873697026 31.44518489936298 20.190506173063074 40-41 20.942964185267193 26.9593650007635 31.5928034487784 20.504867365190897 42-43 20.265191646372603 28.154256599974687 29.78800790950556 21.792543844147154 44-45 21.080518194380872 27.52283035657856 28.786332655414526 22.610318793626043 46-47 21.712768187513102 27.73323703991047 28.162988225412967 22.391006547163464 48-49 22.24065218265613 27.191792281829706 28.633292981643955 21.9342625538702 50-51 21.66541804159062 27.894139111168144 29.601798577246424 20.838644269994816 52-53 21.226107618958743 28.660393560077946 28.796700090116566 21.31679873084675 54-55 19.806667233087126 27.51642155053422 29.617377176134013 23.05953404024464 56-57 21.855931188271196 28.160869910398784 27.689359698812886 22.293839202517134 58-59 22.40785311655155 26.989934633968332 28.101143243954724 22.5010690055254 60-61 21.411767737370543 26.921570238782543 29.83709274055668 21.829569283290237 62-63 22.490734150557344 26.676888930983523 29.187077535068155 21.645299383390974 64-65 21.443808072605815 28.093613284324036 29.252157853025036 21.210420790045113 66-67 21.011649508408503 27.547056805934965 28.779143358073956 22.662150327582577 68-69 20.441352164191866 28.626117805106254 28.61208484820206 22.320445182499824 70-71 21.373784639641865 28.43596763264064 27.429937230611518 22.76031049710598 72-73 21.338701095745023 27.01742021480335 28.063945852061188 23.579932837390434 74-75 20.860940527244594 29.010483189151472 27.732293905362283 22.396282378241654 76-77 21.68757740994915 26.864639788753074 28.59958115194195 22.848201649355826 78-79 21.16110746140347 27.067067848103736 29.39670564925977 22.375119041233027 80-81 21.28578069422512 28.52477597076434 28.328020237931494 21.861423097079044 82-83 20.83489447168989 27.911882851249164 28.277276279007573 22.97594639805337 84-85 20.728011627404907 27.526867829500013 28.001929775934283 23.743190767160797 86-87 21.45487130323975 27.546358697907603 28.14325973669536 22.855510262157292 88-89 21.760990008178336 26.671676376910476 28.10926059262007 23.458073022291114 90-91 21.038023907765975 26.610240278914628 28.90044669920298 23.45128911411642 92-93 20.937268005554635 28.200496128609014 28.389295210450737 22.472940655385614 94-95 20.560265377317048 28.028990518433243 27.930986994206318 23.479757110043384 96-97 19.865361369061706 27.37259846533689 28.117905778377626 24.644134387223783 98-99 21.0795033132184 27.57548322846408 27.093334215465397 24.25167924285212 100-101 21.359675299757853 26.774346424460045 28.114682058542705 23.751296217239393 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9170.0 1 5764.0 2 3294.5 3 4058.0 4 3147.5 5 2831.0 6 5062.0 7 10178.0 8 14244.0 9 14023.0 10 10484.5 11 7290.0 12 6713.0 13 6722.0 14 6762.0 15 6714.5 16 6462.0 17 6411.5 18 6657.0 19 7001.5 20 7525.0 21 8291.0 22 9321.0 23 10499.0 24 11610.0 25 13367.0 26 16120.0 27 19193.0 28 21445.0 29 24145.5 30 27912.0 31 31672.0 32 36877.0 33 42283.5 34 47947.0 35 52652.0 36 57750.5 37 63748.0 38 66294.0 39 66316.5 40 66713.5 41 68080.5 42 68726.0 43 72185.5 44 79427.0 45 84400.0 46 92012.5 47 130167.0 48 249883.0 49 249948.5 50 149545.0 51 113756.5 52 81570.5 53 71699.0 54 69115.5 55 64641.5 56 59166.0 57 53415.0 58 48703.0 59 43762.5 60 38042.0 61 32563.0 62 27067.5 63 20723.0 64 15296.5 65 12338.0 66 9097.0 67 6962.5 68 5156.5 69 3282.5 70 2506.0 71 2063.0 72 1685.5 73 1383.0 74 1087.0 75 875.5 76 606.5 77 316.0 78 193.0 79 136.0 80 73.5 81 40.0 82 19.0 83 9.0 84 5.5 85 4.5 86 4.5 87 3.0 88 2.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37599640090868697 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.7856743908774735E-4 16-17 0.002263360442587947 18-19 6.964185977193684E-5 20-21 1.741046494298421E-5 22-23 0.0 24-25 0.0 26-27 1.741046494298421E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008200328988145562 36-37 0.01831580912001939 38-39 0.01142126500259764 40-41 0.007329805740996352 42-43 0.0035343243834257943 44-45 0.0019673825385572155 46-47 0.0020196139333861682 48-49 8.705232471492105E-5 50-51 3.656197638026684E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.7856743908774735E-4 60-61 6.267767379474316E-4 62-63 0.0 64-65 0.0 66-67 0.001427658125324705 68-69 8.705232471492105E-4 70-71 0.0018803302138422947 72-73 0.0 74-75 0.0 76-77 0.09668031182839132 78-79 0.03419415314802099 80-81 3.482092988596842E-5 82-83 0.0 84-85 0.0 86-87 0.15178443337293634 88-89 0.3271426362786733 90-91 0.28427807158904617 92-93 0.0 94-95 1.741046494298421E-5 96-97 1.741046494298421E-5 98-99 2.0892557931581052E-4 100-101 6.267767379474316E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2871836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.531030887868795 #Duplication Level Percentage of deduplicated Percentage of total 1 66.47670255288071 20.960789615185476 2 14.48541335864039 9.134800320696748 3 6.342826729245794 5.999875965487467 4 3.4730258655283173 4.380323433613625 5 2.2483650488439104 3.544663390115098 6 1.4656903418268206 2.7728836464115467 7 1.006365035472277 2.221220891256328 8 0.7402517654013635 1.8672721023735837 9 0.5690963252409572 1.614977442841072 >10 2.8507587442523743 15.976632959317996 >50 0.20401967565226573 4.47089403858269 >100 0.11932412581861426 6.974752046372639 >500 0.007308466213110723 1.6589835180742913 >1k 0.007529934886235213 5.2745717923435 >5k 0.0017717493849965208 4.032306889587159 >10k+ 0.0015502807118719557 9.115051947740733 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35662 1.2417840015934056 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33516 1.1670582860581176 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 29921 1.041877043118061 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23570 0.8207293174122757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18850 0.6563745283505047 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17568 0.6117340962366932 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17261 0.6010440707617009 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14549 0.5066097089109546 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12997 0.4525676257279315 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12394 0.43157060500669264 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12138 0.4226564469558847 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 11885 0.41384675169473467 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10699 0.3725491288499761 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10049 0.3499155244240966 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9485 0.33027651996841045 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9295 0.32366054329007643 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9231 0.32143200377737446 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8146 0.28365129485109875 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8102 0.28211917393611613 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8065 0.2808307995303353 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7966 0.2773835274716244 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7898 0.27501570423937854 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7109 0.24754199055934947 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6479 0.2256048047311894 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6264 0.2181183048057062 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6176 0.21505406297574098 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5484 0.19095797949465082 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5327 0.18549109350255377 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5281 0.18388933072779923 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5179 0.18033759587943043 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4959 0.17267699130451739 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4870 0.16957792854466622 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4459 0.15526652636153318 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4423 0.1540129728856383 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4389 0.15282906126951537 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4327 0.15067016361658536 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4260 0.14833716131422547 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4201 0.14628272645095333 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3731 0.12991688940454818 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3654 0.12723567780332862 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3490 0.12152504530202979 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3469 0.12079380577442445 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3459 0.12044559647556477 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3345 0.11647601046856436 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3198 0.11135733377532701 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3193 0.11118322912589716 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3072 0.10696989660969498 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2910 0.1013289059681681 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.482092988596842E-5 0.0 10-11 3.482092988596842E-5 0.0 0.0 3.482092988596842E-5 0.0 12-13 3.482092988596842E-5 0.0 0.0 6.964185977193684E-5 0.0 14-15 3.482092988596842E-5 0.0 0.0 1.566941844868579E-4 0.0 16-17 3.482092988596842E-5 0.0 0.0 3.133883689737158E-4 0.0 18-19 3.482092988596842E-5 0.0 0.0 5.397244132325105E-4 3.482092988596842E-5 20-21 5.223139482895263E-5 0.0 0.0 0.001096859291408005 3.482092988596842E-5 22-23 6.964185977193684E-5 0.0 0.0 0.0024896964868467417 3.482092988596842E-5 24-25 6.964185977193684E-5 0.0 0.0 0.004317795305860084 3.482092988596842E-5 26-27 6.964185977193684E-5 0.0 0.0 0.006232946449588347 3.482092988596842E-5 28-29 6.964185977193684E-5 0.0 0.0 0.01277928126815041 3.482092988596842E-5 30-31 6.964185977193684E-5 0.0 0.0 0.030050462491590744 3.482092988596842E-5 32-33 6.964185977193684E-5 0.0 0.0 0.06427943656949771 3.482092988596842E-5 34-35 6.964185977193684E-5 0.0 0.0 0.12279600924286763 3.482092988596842E-5 36-37 6.964185977193684E-5 0.0 0.0 0.2071671223565691 3.482092988596842E-5 38-39 6.964185977193684E-5 0.0 0.0 0.32033514448596645 3.482092988596842E-5 40-41 6.964185977193684E-5 0.0 0.0 0.44671770950708883 3.482092988596842E-5 42-43 6.964185977193684E-5 0.0 0.0 0.5679467769050879 3.482092988596842E-5 44-45 6.964185977193684E-5 0.0 0.0 0.7097027824708653 3.482092988596842E-5 46-47 6.964185977193684E-5 0.0 0.0 0.8941144271469541 3.482092988596842E-5 48-49 1.0446278965790526E-4 3.482092988596842E-5 0.0 1.0967025972235183 3.482092988596842E-5 50-51 1.0446278965790526E-4 3.482092988596842E-5 0.0 1.3101200764946188 3.482092988596842E-5 52-53 1.0446278965790526E-4 3.482092988596842E-5 0.0 1.5090694593980993 3.482092988596842E-5 54-55 1.0446278965790526E-4 3.482092988596842E-5 0.0 1.7183606584777125 3.482092988596842E-5 56-57 1.0446278965790526E-4 3.482092988596842E-5 0.0 1.982616695382327 3.482092988596842E-5 58-59 1.0446278965790526E-4 3.482092988596842E-5 0.0 2.2959180120313274 3.482092988596842E-5 60-61 1.0446278965790526E-4 3.482092988596842E-5 0.0 2.673498765249826 3.482092988596842E-5 62-63 1.0446278965790526E-4 3.482092988596842E-5 0.0 3.014674236272545 3.482092988596842E-5 64-65 1.0446278965790526E-4 3.482092988596842E-5 0.0 3.321046187874238 3.482092988596842E-5 66-67 1.0446278965790526E-4 3.482092988596842E-5 0.0 3.672633116932861 3.482092988596842E-5 68-69 1.0446278965790526E-4 3.482092988596842E-5 0.0 4.079637555905003 3.482092988596842E-5 70-71 1.0446278965790526E-4 3.482092988596842E-5 0.0 4.543121543152186 3.482092988596842E-5 72-73 1.0446278965790526E-4 3.482092988596842E-5 0.0 5.009930929203478 3.482092988596842E-5 74-75 1.0446278965790526E-4 3.482092988596842E-5 0.0 5.472265825764424 3.482092988596842E-5 76-77 1.0446278965790526E-4 3.482092988596842E-5 0.0 5.9193143341054295 3.482092988596842E-5 78-79 1.0446278965790526E-4 3.482092988596842E-5 0.0 6.45506916133094 3.482092988596842E-5 80-81 1.0446278965790526E-4 3.482092988596842E-5 0.0 7.045301333363048 3.482092988596842E-5 82-83 1.0446278965790526E-4 3.482092988596842E-5 0.0 7.625801055492026 3.482092988596842E-5 84-85 1.0446278965790526E-4 3.482092988596842E-5 0.0 8.28724202914094 3.482092988596842E-5 86-87 1.0446278965790526E-4 3.482092988596842E-5 0.0 8.968182723525995 3.482092988596842E-5 88-89 1.0446278965790526E-4 3.482092988596842E-5 0.0 9.611638686888806 3.482092988596842E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 125 5.7152647E-9 34.1655 2 TACCTGG 2010 0.0 26.440964 2 GCACCGT 435 0.0 23.998756 6 CATGGGG 6265 0.0 22.040794 4 GAGTACT 17700 0.0 21.48898 12-13 AGTACTT 17890 0.0 21.154654 12-13 ATGGGAT 5405 0.0 20.89472 5 GTACTTT 18825 0.0 20.116547 14-15 TGCACCG 450 0.0 20.035324 5 GTAAGCG 355 0.0 19.382008 94-95 GTATAGG 795 0.0 18.573503 1 CACGCGT 130 0.0076099755 18.250803 7 GCTTATG 575 0.0 18.224436 1 TACTTTT 20690 0.0 18.211493 14-15 GTACATG 33760 0.0 18.200634 1 TGTACCG 235 2.554605E-6 18.17314 5 ACTTTTT 21185 0.0 18.087748 16-17 TACATGG 33910 0.0 17.92572 2 ACATGGG 33105 0.0 17.630589 3 CATACCG 135 0.0094585335 17.574846 5 >>END_MODULE