##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938746_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2867506 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.005850380086386 31.0 31.0 34.0 30.0 34.0 2 32.17652831415174 33.0 31.0 34.0 30.0 34.0 3 32.258815151563766 34.0 31.0 34.0 30.0 34.0 4 35.861257831718575 37.0 35.0 37.0 35.0 37.0 5 35.78562730121576 37.0 35.0 37.0 35.0 37.0 6 35.853252966166416 37.0 35.0 37.0 35.0 37.0 7 35.77451346222118 37.0 35.0 37.0 33.0 37.0 8 35.786134013320286 37.0 35.0 37.0 33.0 37.0 9 37.387833538970796 39.0 37.0 39.0 34.0 39.0 10-11 37.3080267312431 39.0 37.0 39.0 34.0 39.0 12-13 37.23090204519188 39.0 37.0 39.0 33.0 39.0 14-15 38.29992945088868 40.0 38.0 41.0 33.0 41.0 16-17 38.25584235917902 40.0 37.5 41.0 33.0 41.0 18-19 38.50242545264072 40.0 38.0 41.0 34.0 41.0 20-21 38.51720484630198 40.0 38.0 41.0 34.0 41.0 22-23 38.451733318081985 40.0 38.0 41.0 34.0 41.0 24-25 38.34595097621417 40.0 38.0 41.0 33.5 41.0 26-27 38.08266939981991 40.0 37.5 41.0 33.0 41.0 28-29 37.89204015614963 40.0 37.0 41.0 33.0 41.0 30-31 37.67608385126309 40.0 37.0 41.0 33.0 41.0 32-33 37.47021244244999 40.0 36.0 41.0 32.5 41.0 34-35 37.40296864243702 40.0 36.0 41.0 32.0 41.0 36-37 37.223596567888606 40.0 35.5 41.0 31.5 41.0 38-39 37.03771849126035 39.5 35.0 41.0 31.0 41.0 40-41 36.85304477130998 39.0 35.0 41.0 31.0 41.0 42-43 36.64787449442129 39.0 35.0 41.0 30.5 41.0 44-45 36.35565243804198 39.0 35.0 41.0 30.0 41.0 46-47 35.935616874036185 39.0 35.0 41.0 29.0 41.0 48-49 35.55522342411838 39.0 35.0 41.0 27.5 41.0 50-51 35.29984331331826 39.0 35.0 40.0 27.0 41.0 52-53 35.027092358307186 38.0 34.5 40.0 26.0 41.0 54-55 34.77781371686755 38.0 34.0 40.0 26.0 41.0 56-57 34.53315459496859 38.0 34.0 40.0 24.5 41.0 58-59 34.25300103992808 38.0 34.0 40.0 24.0 41.0 60-61 33.804180706160686 37.0 33.0 40.0 22.5 41.0 62-63 33.409797398854614 36.5 33.0 39.5 22.0 41.0 64-65 32.95058946694445 36.0 32.0 39.0 21.5 40.5 66-67 32.497786403934285 35.5 32.0 39.0 20.0 40.0 68-69 32.17640067710408 35.0 31.5 38.0 19.5 40.0 70-71 31.68835479332912 35.0 31.0 37.5 18.0 39.5 72-73 31.14553709739404 35.0 31.0 37.0 14.0 39.0 74-75 30.652519122889366 34.5 30.0 36.0 9.5 39.0 76-77 28.69894954012302 32.5 27.5 34.5 7.0 36.5 78-79 29.633675395971274 34.0 29.0 35.0 5.0 37.0 80-81 29.690026803780007 34.0 29.5 35.0 2.0 37.0 82-83 29.376357015469193 34.0 29.0 35.0 2.0 36.5 84-85 28.99307987498544 34.0 29.0 35.0 2.0 36.0 86-87 28.412497654756432 34.0 29.0 35.0 2.0 35.5 88-89 27.736968292306972 33.0 26.5 35.0 2.0 35.0 90-91 27.144406498190413 33.0 25.5 35.0 2.0 35.0 92-93 26.87455196257654 33.0 25.0 35.0 2.0 35.0 94-95 26.54027977622366 33.0 25.0 35.0 2.0 35.0 96-97 25.83944410229656 32.0 24.0 34.5 2.0 35.0 98-99 24.960209150390618 32.0 18.0 34.0 2.0 35.0 100-101 21.28505432944168 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12927001328174725 1101 2 0.12377061100521303 1101 3 0.11166641038159497 1101 4 0.05775948200796677 1101 5 -0.13362343426138068 1101 6 0.024142522973775726 1101 7 0.2139821749547366 1101 8 0.21905405667742883 1101 9 0.25167426807831816 1101 10-11 0.20334265631748139 1101 12-13 0.16969668191401865 1101 14-15 0.29765095691892185 1101 16-17 0.3820878250658737 1101 18-19 0.3252240272657545 1101 20-21 0.2809180495983483 1101 22-23 0.17739431733090072 1101 24-25 0.2328669690297076 1101 26-27 0.1724163480091505 1101 28-29 0.202632549071474 1101 30-31 0.2064276118236421 1101 32-33 0.19590570066777957 1101 34-35 0.30607922193431847 1101 36-37 0.30272052344537315 1101 38-39 0.4196873394352707 1101 40-41 0.41034223937477066 1101 42-43 0.46876469213455607 1101 44-45 0.4530165090665008 1101 46-47 0.43949495137417927 1101 48-49 0.4049852159223022 1101 50-51 0.3181077784119921 1101 52-53 0.31552050508350504 1101 54-55 0.3737233794432413 1101 56-57 0.39558360702830697 1101 58-59 0.45373831970764655 1101 60-61 0.5206213844480274 1101 62-63 0.4537358050690088 1101 64-65 0.491566186126974 1101 66-67 0.4853072454406515 1101 68-69 0.5104796085908916 1101 70-71 0.5305322365189653 1101 72-73 0.38361828152610755 1101 74-75 0.40683712095477276 1101 76-77 0.3502424373869637 1101 78-79 0.10036866680168544 1101 80-81 0.37921491166287424 1101 82-83 0.45384834616235636 1101 84-85 0.4305672412618371 1101 86-87 0.5578577081091076 1101 88-89 0.6612112347368644 1101 90-91 0.4769552680226745 1101 92-93 0.5734983461245662 1101 94-95 0.7428577984269715 1101 96-97 0.6842069305042564 1101 98-99 0.5172904192312551 1101 100-101 1.112407036717478 1102 1 0.21839790566609452 1102 2 0.1491167130045028 1102 3 0.23170044015294167 1102 4 0.15899067720260263 1102 5 0.004349335309868252 1102 6 -0.019646840319587966 1102 7 0.0812341526713638 1102 8 0.12611796790402252 1102 9 0.22042241649206318 1102 10-11 0.27707628575203813 1102 12-13 0.2539216374695954 1102 14-15 0.36749997428448467 1102 16-17 0.3194040173831638 1102 18-19 0.2738713552980556 1102 20-21 0.35632697520405543 1102 22-23 0.25066875337427774 1102 24-25 0.23806704969211268 1102 26-27 0.24339055765359063 1102 28-29 0.3092794671800547 1102 30-31 0.2027620749901402 1102 32-33 0.1635266860155511 1102 34-35 0.2827540667316484 1102 36-37 0.29279538538219896 1102 38-39 0.24779821413670078 1102 40-41 0.2619215009984117 1102 42-43 0.28805400596752406 1102 44-45 0.27818468077876446 1102 46-47 0.2843006813549849 1102 48-49 0.1641177797077873 1102 50-51 0.17168478240365914 1102 52-53 0.0773395059631099 1102 54-55 0.13652637524304367 1102 56-57 0.16964431083069798 1102 58-59 0.132449038424447 1102 60-61 0.05184736150705049 1102 62-63 -0.01994128184829691 1102 64-65 0.12437797290866826 1102 66-67 0.36591257714446357 1102 68-69 0.22454167032916672 1102 70-71 0.28375962288147605 1102 72-73 0.17809697528333857 1102 74-75 0.2760015886044975 1102 76-77 0.3699841930759895 1102 78-79 0.1820562021963852 1102 80-81 0.3363874831890925 1102 82-83 0.37352618477600075 1102 84-85 0.6521866357394117 1102 86-87 0.6788158463027294 1102 88-89 0.5323344104555439 1102 90-91 0.6614259616558122 1102 92-93 0.8234013325408824 1102 94-95 0.8516782403969536 1102 96-97 0.8694195301148291 1102 98-99 0.9328824983455171 1102 100-101 1.5039305924547648 1103 1 0.20361179260087425 1103 2 0.14992982761138052 1103 3 0.09665144622479716 1103 4 0.015876020434511418 1103 5 -0.016637852428679878 1103 6 -0.01761935482568333 1103 7 -0.04715575817165529 1103 8 -0.05225697534384466 1103 9 0.0994369749729529 1103 10-11 0.09568755485443603 1103 12-13 0.1520094024583969 1103 14-15 0.23099625483708053 1103 16-17 0.14363954746054475 1103 18-19 0.030003204843787046 1103 20-21 0.0703688246001235 1103 22-23 0.12756658207513993 1103 24-25 0.14577342788332004 1103 26-27 0.16298020855938233 1103 28-29 0.08503614148020233 1103 30-31 0.15724811271845596 1103 32-33 0.20063141677346152 1103 34-35 0.2245010594923258 1103 36-37 0.10520427014668599 1103 38-39 0.1491256527321383 1103 40-41 0.10671115639447493 1103 42-43 0.044641182230911625 1103 44-45 0.0870599657867217 1103 46-47 0.1359536027800985 1103 48-49 0.11142018250573216 1103 50-51 0.021519328112198366 1103 52-53 -0.04460785300472736 1103 54-55 -0.07386213363523098 1103 56-57 -0.1676409665437788 1103 58-59 -0.06489015971158807 1103 60-61 -0.06098167612647387 1103 62-63 -0.09158473626947483 1103 64-65 -0.0610452108983921 1103 66-67 -0.1357506345023225 1103 68-69 -0.16108444912222808 1103 70-71 -0.14204114345939445 1103 72-73 0.021131804877267513 1103 74-75 0.08565487673569194 1103 76-77 0.16308001562018504 1103 78-79 -0.028931758867908997 1103 80-81 0.13230114261643422 1103 82-83 0.09676830308985274 1103 84-85 0.16986685805809287 1103 86-87 0.2697162968273439 1103 88-89 0.14189508659170968 1103 90-91 0.044157223473977325 1103 92-93 -0.02159861756713255 1103 94-95 0.16619531093923 1103 96-97 0.2574004888391883 1103 98-99 0.37375394915451565 1103 100-101 0.9234332442137116 1104 1 0.10317862154805368 1104 2 0.11712194087694883 1104 3 0.033199808599952974 1104 4 5.834979469483415E-4 1104 5 -0.07594691573773105 1104 6 -0.01645936032782913 1104 7 0.06837785157892995 1104 8 0.021012551127853385 1104 9 0.09575139796012166 1104 10-11 0.04242157775711064 1104 12-13 -0.07074552613655527 1104 14-15 -0.08077563215280747 1104 16-17 0.06990098774507914 1104 18-19 0.15313100197186458 1104 20-21 0.1074127484150722 1104 22-23 0.0763248537140484 1104 24-25 0.0027419305631681823 1104 26-27 -0.00955015148718985 1104 28-29 -0.021019594750931958 1104 30-31 -0.19765493655030042 1104 32-33 -0.12380774523030169 1104 34-35 -0.09582973237431958 1104 36-37 -0.09121712533467985 1104 38-39 -0.11147512493700873 1104 40-41 0.04376797586027692 1104 42-43 0.05220865718878542 1104 44-45 0.010053525330235402 1104 46-47 -0.020423480788629433 1104 48-49 -0.01944346993560231 1104 50-51 -0.10767415332051655 1104 52-53 0.01594017531166969 1104 54-55 0.057377273508713245 1104 56-57 -0.02012503734459159 1104 58-59 0.04159764223086171 1104 60-61 0.05214247337464428 1104 62-63 0.08887165923645313 1104 64-65 -0.04501370620928924 1104 66-67 -0.0965576197693494 1104 68-69 -0.05731351518235073 1104 70-71 -0.018094411094505602 1104 72-73 0.10329306683337691 1104 74-75 0.0668523423104439 1104 76-77 0.036129456317382846 1104 78-79 0.024132247773966498 1104 80-81 0.15830362595033876 1104 82-83 0.29570011334447344 1104 84-85 0.176701307376959 1104 86-87 0.22964619361301075 1104 88-89 0.24926955664186679 1104 90-91 0.25582877001867743 1104 92-93 0.4465841273151341 1104 94-95 0.3916780995438991 1104 96-97 0.27953464536537354 1104 98-99 0.4498975477534337 1104 100-101 1.1462304214171475 1105 1 0.11740508504318115 1105 2 0.14435607029751907 1105 3 0.08982684242767647 1105 4 0.08331357554726537 1105 5 -0.04629558088171848 1105 6 -0.00649144937847268 1105 7 0.021562970666259673 1105 8 0.02880238322889994 1105 9 0.16424994480161104 1105 10-11 0.1404190795961071 1105 12-13 0.1443668157029876 1105 14-15 0.18324461180699103 1105 16-17 0.12304138219778338 1105 18-19 0.17361463127160448 1105 20-21 0.06508360237086208 1105 22-23 0.07064776895428793 1105 24-25 0.10992607987274283 1105 26-27 0.11974421877076935 1105 28-29 0.07496463495923678 1105 30-31 0.10226445776208237 1105 32-33 0.10040310988612333 1105 34-35 0.15693111196210907 1105 36-37 0.20509733312503897 1105 38-39 0.26148887565319967 1105 40-41 0.21948306630429926 1105 42-43 0.2665494478049837 1105 44-45 0.2948997405125624 1105 46-47 0.18045328854602616 1105 48-49 0.3157453465651443 1105 50-51 0.358167120435148 1105 52-53 0.4805487768470229 1105 54-55 0.46374413350758203 1105 56-57 0.6151438781174008 1105 58-59 0.5495112000206532 1105 60-61 0.4901946920097018 1105 62-63 0.47145628223866964 1105 64-65 0.508879056254159 1105 66-67 0.5165891304798222 1105 68-69 0.4951356803714404 1105 70-71 0.43644149571706947 1105 72-73 0.41153719119030185 1105 74-75 0.412169472786573 1105 76-77 0.6228615864007985 1105 78-79 0.4439644561948626 1105 80-81 0.5335784797628023 1105 82-83 0.4755775386484835 1105 84-85 0.6095879935879793 1105 86-87 0.8338241887308264 1105 88-89 0.7776160710943678 1105 90-91 0.7419611170533678 1105 92-93 0.7726578412828182 1105 94-95 1.0882447991517985 1105 96-97 1.239175217111974 1105 98-99 1.3582715267752015 1105 100-101 1.925947238591597 1106 1 0.09356828796607175 1106 2 0.031055545580024102 1106 3 9.322454210476394E-4 1106 4 0.031162354860988728 1106 5 0.008645543776275133 1106 6 0.022470782228133146 1106 7 0.09058664510398273 1106 8 0.022283335159826834 1106 9 0.13129250925535985 1106 10-11 0.11980537753750298 1106 12-13 0.06216759251393711 1106 14-15 0.13540406102819702 1106 16-17 0.17483919487396093 1106 18-19 -0.014006614502982018 1106 20-21 -0.04005001413891307 1106 22-23 0.026865911023257638 1106 24-25 0.08071670125166719 1106 26-27 0.16670535215311588 1106 28-29 0.19294256386375963 1106 30-31 0.18417312172268652 1106 32-33 0.15999841959794736 1106 34-35 0.0638315241104479 1106 36-37 0.010275071807257063 1106 38-39 0.21184933669324835 1106 40-41 0.2618757915810974 1106 42-43 0.23966966402341683 1106 44-45 0.22182860453712294 1106 46-47 0.29885713433280614 1106 48-49 0.2622094741099943 1106 50-51 0.23553535563670636 1106 52-53 0.2346658375910735 1106 54-55 0.28179394279644043 1106 56-57 0.2761058345860121 1106 58-59 0.2642269047207648 1106 60-61 0.3311555009061067 1106 62-63 0.43646272074717984 1106 64-65 0.3827764674895704 1106 66-67 0.3144557518643296 1106 68-69 0.2721814314911981 1106 70-71 0.2650783568533832 1106 72-73 0.2852068337855762 1106 74-75 0.3411168883748381 1106 76-77 0.3548117654732721 1106 78-79 0.38932075757665174 1106 80-81 0.3415003824700946 1106 82-83 0.40065457496064383 1106 84-85 0.5026450886800475 1106 86-87 0.5746264441175448 1106 88-89 0.5841552424122334 1106 90-91 0.40079855192922764 1106 92-93 0.5497456661702849 1106 94-95 0.7092715340924158 1106 96-97 0.8249262693635195 1106 98-99 0.9249489048916288 1106 100-101 1.410326406687883 1107 1 -0.06219453089719451 1107 2 -0.01747674386700737 1107 3 -0.05361445454433067 1107 4 -0.034347743054262025 1107 5 -0.06855921554548416 1107 6 -0.027972048705606767 1107 7 -0.025759222146703564 1107 8 -0.03169640421418052 1107 9 -0.03851612187498432 1107 10-11 -0.1595536931840016 1107 12-13 -0.07831829965525827 1107 14-15 -0.018453538835544236 1107 16-17 -0.022361963669510487 1107 18-19 -0.001035018997193049 1107 20-21 -0.043984489230595614 1107 22-23 -0.07259494416621237 1107 24-25 -0.0506432081086885 1107 26-27 0.010644011182549207 1107 28-29 0.06692897717386614 1107 30-31 -0.05370225247130378 1107 32-33 -0.04129202899053297 1107 34-35 0.0540082954804717 1107 36-37 -0.017140596854503087 1107 38-39 -0.03560975420742807 1107 40-41 -0.047393111579253855 1107 42-43 -0.06062621998153617 1107 44-45 -0.02716984222226415 1107 46-47 -0.06457432406570973 1107 48-49 0.028008467373119572 1107 50-51 -0.1042263080154342 1107 52-53 -0.09190138033099515 1107 54-55 0.03198138691032426 1107 56-57 0.033141236993806444 1107 58-59 0.15163177104765424 1107 60-61 0.07740708145338715 1107 62-63 0.05336096960262182 1107 64-65 0.09548897357571917 1107 66-67 -0.006376833235300694 1107 68-69 0.003980839445496542 1107 70-71 -0.06334178084630082 1107 72-73 -0.03352799712594745 1107 74-75 -0.06974232767370836 1107 76-77 -0.054375958186895446 1107 78-79 0.10071884074074333 1107 80-81 0.1964197953858715 1107 82-83 0.14709498809672894 1107 84-85 0.08033627704796231 1107 86-87 0.06801336949720138 1107 88-89 0.06818778085910182 1107 90-91 0.437658395568441 1107 92-93 0.636675303815359 1107 94-95 0.503377828326915 1107 96-97 0.6539207728541463 1107 98-99 0.8422272718970198 1107 100-101 1.0762956732584037 1108 1 0.0888242475478691 1108 2 0.017064086895068442 1108 3 0.02314089999499913 1108 4 0.10542636106955428 1108 5 -0.0011835492397409553 1108 6 0.0669081360845567 1108 7 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9 2508.0 10 4084.0 11 6254.0 12 9087.0 13 12598.0 14 14694.0 15 19624.0 16 25528.0 17 35354.0 18 32457.0 19 21371.0 20 19109.0 21 20394.0 22 21669.0 23 23856.0 24 27453.0 25 32096.0 26 38234.0 27 45825.0 28 55716.0 29 66573.0 30 80735.0 31 96664.0 32 118460.0 33 148904.0 34 199305.0 35 289246.0 36 404837.0 37 565876.0 38 396532.0 39 29842.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.26170543719524 18.930178093923132 14.34094762046245 20.467168848419178 2 14.719690211633385 23.333656494528697 38.3162581002446 23.63039519359332 3 22.649298728581563 27.078827385191172 29.581420230681292 20.69045365554597 4 12.465850115047711 16.821795664943686 37.94175147323144 32.770602746777165 5 12.235553067286176 40.514862467886495 33.093901356397495 14.155683108429825 6 28.690645956550746 36.80342164706523 17.656782768725996 16.849149627658036 7 26.63446911706549 35.292271402396366 20.459137661786933 17.614121818751208 8 25.32992084410634 32.245965657962 19.985660012568413 22.438453485363237 9 26.35429533538901 19.00299423959357 21.426110355130902 33.21660006988652 10-11 24.667324148580683 26.41786974464918 26.342734766727606 22.572071340042534 12-13 29.262850714174615 24.13117880136955 26.21108726537974 20.39488321907609 14-15 24.768457328424073 25.177471293870003 28.226113563493847 21.82795781421207 16-17 20.476638116576368 28.89444208750281 29.15354291920968 21.475376876711145 18-19 20.073000719091784 29.37069704474899 30.789246822848842 19.767055413310384 20-21 21.973537282921114 26.755968426918724 29.241420942100905 22.029073348059256 22-23 20.7486749809765 26.740763576431924 28.977428469199367 23.53313297339221 24-25 22.217006670462087 26.96233730341056 29.623076607872555 21.1975794182548 26-27 22.796614986836797 24.983739981998024 31.605464482017727 20.614180549147452 28-29 20.567454784750232 27.12114638992909 31.730604922884208 20.580793902436472 30-31 20.74182233620435 27.71943633247847 30.495245694342053 21.043495636975127 32-33 20.559991853547995 27.665051093179926 30.203650838045327 21.571306215226752 34-35 21.890640179164162 26.12596211542194 30.166258357776936 21.81713934763696 36-37 22.574696494805373 25.765102361281922 31.153030238555818 20.507170905356887 38-39 22.67062488413878 25.70311520411368 31.40407144393032 20.22218846781722 40-41 20.96752114514683 26.952038449177447 31.586018106798967 20.494422298876753 42-43 20.26934628360616 28.164367184109096 29.7580403417809 21.80824619050384 44-45 21.08707050948169 27.49912856427673 28.774973237899427 22.638827688342154 46-47 21.704926093772066 27.745480795889083 28.14956311890036 22.40002999143849 48-49 22.25885368533017 27.15843345132435 28.62531168808523 21.957401175260248 50-51 21.671609988474298 27.899166697181 29.55846633222959 20.87075698211511 52-53 21.26938879988394 28.610873002532518 28.83293007930934 21.28680811827421 54-55 19.801214016640245 27.527544842103207 29.603059243816755 23.068181897439796 56-57 21.880709517201744 28.133536485634902 27.675920698064598 22.309833299098752 58-59 22.40585333395641 27.01247151694127 28.03894271443986 22.54273243466246 60-61 21.44060310431184 26.952767128485394 29.784465607018966 21.822164160183803 62-63 22.50016608628012 26.70266336133173 29.14110638048562 21.656064171902525 64-65 21.45487276428371 28.07953119379268 29.239009455602332 21.22658658632128 66-67 21.05269582370182 27.555379161894507 28.759201959736675 22.632723054667 68-69 20.47735674886024 28.60586873787925 28.597830374134393 22.318944139126117 70-71 21.37875705796995 28.37301303608052 27.503877066562787 22.744352839386742 72-73 21.338351166484046 26.966517245299578 28.066270829075858 23.628860759140522 74-75 20.911755577177253 28.966918803552932 27.697969304164964 22.423356315104847 76-77 21.727975043966165 26.854220752030816 28.57594980878207 22.84185439522095 78-79 21.156247064341258 27.09520910970048 29.319657048706528 22.42888677725173 80-81 21.30522755718703 28.504413461729598 28.29011357370957 21.9002454073738 82-83 20.906471726660392 27.826536222303865 28.24835251713133 23.018639533904413 84-85 20.771970485850773 27.491258954645602 27.988293660065576 23.74847689943805 86-87 21.50406592048572 27.45931112198739 28.147815601935665 22.888807355591226 88-89 21.809724723509127 26.662392411610764 28.05305349094882 23.474829373931293 90-91 21.080458482414805 26.57605080556509 28.844078869058038 23.49941184296207 92-93 20.96466180202072 28.157707153149858 28.361334573906394 22.516296470923024 94-95 20.577224465370158 28.018955898138376 27.881466700238327 23.52235293625314 96-97 19.854476125910285 27.378742891896852 28.080050162127172 24.686730820065687 98-99 21.089668164600177 27.551485506917857 27.020588623005498 24.338257705476465 100-101 21.464603664830904 26.597644196700088 28.13959473710641 23.798157401362594 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8854.0 1 5599.0 2 3240.5 3 4022.5 4 3099.5 5 2790.0 6 5065.5 7 10075.5 8 13967.0 9 13888.5 10 10565.0 11 7262.5 12 6617.5 13 6704.5 14 6722.5 15 6557.5 16 6367.0 17 6354.5 18 6519.0 19 6893.0 20 7429.5 21 8241.0 22 9208.5 23 10352.5 24 11652.5 25 13401.5 26 16091.5 27 18940.5 28 21098.0 29 24218.0 30 28093.5 31 31668.5 32 36955.5 33 42519.5 34 47896.0 35 52270.0 36 57545.5 37 63538.5 38 66157.0 39 66385.0 40 66660.5 41 67931.0 42 68508.5 43 72200.5 44 79051.5 45 83875.5 46 92444.0 47 130499.5 48 248290.0 49 248583.0 50 149298.5 51 113703.5 52 81912.0 53 71896.5 54 69210.5 55 64464.5 56 59021.0 57 53694.0 58 49133.0 59 44206.5 60 38364.5 61 32653.5 62 27180.5 63 20826.0 64 15450.0 65 12217.0 66 8977.0 67 7017.0 68 5174.0 69 3366.0 70 2576.0 71 2053.0 72 1672.0 73 1393.5 74 1095.5 75 885.0 76 615.5 77 330.5 78 213.5 79 153.5 80 110.0 81 72.0 82 52.5 83 33.5 84 14.5 85 11.5 86 7.0 87 2.5 88 1.5 89 1.5 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23211808449572555 2 0.0 3 0.0 4 0.0 5 0.02514380091968421 6 0.004010453683444777 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0031211791710287614 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.7079238892612605E-4 26-27 0.0014995609425054385 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006678277220692826 36-37 0.017576249186575375 38-39 0.013618105768566832 40-41 0.008387079224943209 42-43 0.008038344122034967 44-45 0.003888396397426893 46-47 7.323437161073071E-4 48-49 0.0019529165762861525 50-51 3.487351029082415E-5 52-53 0.0 54-55 0.0 56-57 0.0012205728601788453 58-59 4.1848212348988983E-4 60-61 0.0026155132718118116 62-63 7.149069609618951E-4 64-65 0.001830859290268268 66-67 0.006346978872929995 68-69 2.4411457203576907E-4 70-71 1.0462053087247246E-4 72-73 0.0 74-75 5.579761646531864E-4 76-77 0.058099268144513036 78-79 0.13980790275591404 80-81 0.0010113317984339005 82-83 8.892745124160158E-4 84-85 0.0 86-87 0.10336508450200278 88-89 0.35180397181383405 90-91 0.3880026754957095 92-93 4.8822914407153814E-4 94-95 2.789880823265932E-4 96-97 1.394940411632966E-4 98-99 0.0 100-101 0.001185699349888021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2867506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.361478909146406 #Duplication Level Percentage of deduplicated Percentage of total 1 66.12977001966178 20.73927387738325 2 14.69505951820248 9.217175982975165 3 6.451865859329409 6.070201652160031 4 3.4861662777097853 4.373253207686915 5 2.214793159439819 3.472959447894681 6 1.4432891948314885 2.7158210186103977 7 1.010210051009976 2.2177176845989957 8 0.7531203571282087 1.8895174556900103 9 0.5635954220948785 1.5907667348987995 >10 2.91184588029619 16.216599842496564 >50 0.20319213887228094 4.469832765437565 >100 0.1185836618101779 6.9011527584751295 >500 0.0074702818921677785 1.6486953730613516 >1k 0.007581778636826889 5.137006092395348 >5k 0.0018954446592067222 4.222688242664536 >10k+ 0.0015609544252290655 9.11733786357127 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35467 1.23685878948466 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 33404 1.16491473775469 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 29762 1.0379054132755083 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23313 0.8130061454099835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18603 0.6487519119402017 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17525 0.6111582678466932 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 17391 0.6064852174677229 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14605 0.5093276177974867 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 12915 0.4503913854059939 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12612 0.43982471178787413 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 12188 0.4250383434245647 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12109 0.42228333611158964 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 10790 0.3762851760379926 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10057 0.3507228929948185 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 9635 0.3360062716520907 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9133 0.318499769486097 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9103 0.31745356417737225 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8144 0.2840098678084719 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8093 0.2822313187836399 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8074 0.2815687220881142 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8047 0.28062713731026195 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 7993 0.27874396775455745 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7227 0.25203085887178617 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 6598 0.23009542089885773 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6177 0.2154136730664208 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6087 0.2122750571402466 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 5581 0.1946290609330896 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 5350 0.1865732800559092 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 5258 0.1833649171091534 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5257 0.18333004359886257 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 5005 0.17454191900557486 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4751 0.16568404739170556 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 4710 0.16425423346978174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4577 0.15961605660110215 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4461 0.15557072940736655 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 4279 0.14922375053443654 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4157 0.144969182278956 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 4130 0.14402759750110375 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3823 0.13332142984182074 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3641 0.12697445096889073 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3486 0.12156905687381299 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3441 0.1199997489107259 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3313 0.1155359395935004 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3301 0.11511745747001052 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3124 0.10894484614853465 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3071 0.10709655010312097 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3040 0.10601547128410542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.97470205816483E-5 0.0 16-17 0.0 0.0 0.0 2.2667781689035698E-4 0.0 18-19 0.0 0.0 0.0 4.5335563378071396E-4 0.0 20-21 0.0 0.0 0.0 0.0010462053087247245 0.0 22-23 0.0 0.0 0.0 0.00226677816890357 0.0 24-25 0.0 0.0 0.0 0.004359188786353019 0.0 26-27 0.0 0.0 0.0 0.006695713975838237 0.0 28-29 0.0 0.0 0.0 0.013461174972258122 0.0 30-31 0.0 0.0 0.0 0.029956345339817947 0.0 32-33 0.0 0.0 0.0 0.0620922850728124 0.0 34-35 0.0 0.0 0.0 0.11773297074182233 0.0 36-37 0.0 0.0 0.0 0.19788973414528166 0.0 38-39 0.0 0.0 0.0 0.303730837877933 0.0 40-41 0.0 0.0 0.0 0.42659021463250646 0.0 42-43 0.0 0.0 0.0 0.5444103691500558 0.0 44-45 0.0 0.0 0.0 0.6836254222310258 0.0 46-47 0.0 0.0 0.0 0.8656477091939825 0.0 48-49 0.0 0.0 0.0 1.0565801780362447 0.0 50-51 0.0 0.0 0.0 1.2602414781346578 0.0 52-53 0.0 0.0 0.0 1.458880992751192 0.0 54-55 0.0 0.0 0.0 1.6680523074755556 0.0 56-57 0.0 0.0 0.0 1.9224196915368268 0.0 58-59 0.0 0.0 0.0 2.2270921141926117 0.0 60-61 0.0 0.0 0.0 2.6008838342448106 0.0 62-63 0.0 0.0 0.0 2.9399589748024937 0.0 64-65 0.0 0.0 0.0 3.248973149489487 0.0 66-67 0.0 0.0 0.0 3.601178166671665 0.0 68-69 0.0 0.0 0.0 3.997812035964354 0.0 70-71 0.0 0.0 0.0 4.455038629387349 0.0 72-73 0.0 0.0 0.0 4.913189370833051 0.0 74-75 0.0 0.0 0.0 5.366405510572601 3.487351029082415E-5 76-77 0.0 0.0 0.0 5.808619057815397 3.487351029082415E-5 78-79 0.0 0.0 0.0 6.343212533818587 3.487351029082415E-5 80-81 0.0 0.0 0.0 6.933342074959913 3.487351029082415E-5 82-83 0.0 0.0 0.0 7.509243223902583 3.487351029082415E-5 84-85 0.0 0.0 0.0 8.164882654125222 3.487351029082415E-5 86-87 0.0 0.0 0.0 8.84294575146486 3.487351029082415E-5 88-89 0.0 0.0 0.0 9.47870728082173 3.487351029082415E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 100 3.0569936E-8 37.96666 2 TAGGACG 105 0.0022082517 22.599201 4 TACCTGG 2015 0.0 22.13937 2 CATGGGG 6320 0.0 21.401302 4 AGTACTT 17755 0.0 21.282665 12-13 GAGTACT 17620 0.0 20.759142 12-13 GTACTTT 18740 0.0 19.493153 14-15 ATATAGG 1080 0.0 19.334873 2 GCTTATG 640 0.0 18.586391 1 ATGGGAT 5415 0.0 18.317251 5 GTACATG 33175 0.0 18.315342 1 TACTTTT 20605 0.0 18.189276 14-15 TACATGG 33510 0.0 17.83051 2 ACTTTTT 20830 0.0 17.730572 16-17 ACATGGG 32370 0.0 17.578787 3 ATGGGAG 4530 0.0 17.495674 5 GTGTAGC 935 0.0 17.302242 1 GCTTATA 3690 0.0 17.14985 1 GTATAGA 630 0.0 16.615644 1 ATGGGGA 3470 0.0 16.548868 5 >>END_MODULE