##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938745_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653316 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.356496702973754 31.0 31.0 34.0 30.0 34.0 2 31.220091043231758 31.0 31.0 34.0 28.0 34.0 3 31.31654666348291 33.0 31.0 34.0 28.0 34.0 4 34.673736752199545 37.0 35.0 37.0 32.0 37.0 5 34.774923620422584 37.0 35.0 37.0 32.0 37.0 6 35.01416160020572 37.0 35.0 37.0 32.0 37.0 7 34.990047695142934 37.0 35.0 37.0 32.0 37.0 8 35.01672238243055 37.0 35.0 37.0 32.0 37.0 9 36.385771969460414 39.0 37.0 39.0 32.0 39.0 10-11 36.28086408414917 39.0 36.0 39.0 32.0 39.0 12-13 36.29115619393984 39.0 37.0 39.0 32.0 39.0 14-15 37.043681005822606 39.0 36.5 41.0 31.0 41.0 16-17 37.165471226787645 39.5 36.5 41.0 31.5 41.0 18-19 37.24593611667248 40.0 37.0 41.0 32.0 41.0 20-21 37.00573152961201 39.5 36.0 41.0 31.0 41.0 22-23 37.02940766795854 39.0 36.0 41.0 31.0 41.0 24-25 36.92375894666593 39.5 36.5 41.0 30.5 41.0 26-27 36.714773555216766 39.0 36.0 41.0 30.0 41.0 28-29 36.75049516619829 39.0 36.0 41.0 30.0 41.0 30-31 36.81608670230027 39.5 36.0 41.0 30.0 41.0 32-33 36.51349193958207 39.0 35.0 41.0 30.0 41.0 34-35 36.00257073146838 39.0 35.0 41.0 30.0 41.0 36-37 35.60892661437956 38.5 35.0 40.5 27.5 41.0 38-39 35.41319131936153 38.0 35.0 40.0 27.0 41.0 40-41 35.652032094729044 39.0 35.0 40.0 28.5 41.0 42-43 35.32348358221749 38.0 35.0 40.0 26.5 41.0 44-45 35.25869181223175 38.5 35.0 40.5 25.5 41.0 46-47 35.04113017284132 38.5 35.0 40.5 24.0 41.0 48-49 34.64859807505097 38.5 34.0 40.0 23.0 41.0 50-51 33.333058581146034 37.0 32.5 39.0 22.5 40.5 52-53 33.69569626337025 37.5 33.5 39.5 23.0 40.0 54-55 34.40443598503634 38.0 34.0 40.0 22.5 41.0 56-57 34.101679738442044 38.0 33.5 40.0 20.5 41.0 58-59 33.90613041774577 38.0 34.0 40.0 19.5 41.0 60-61 33.18415131421854 38.0 32.0 40.0 13.5 41.0 62-63 33.021411537448955 37.0 32.5 40.0 13.0 41.0 64-65 32.59103710914779 36.5 32.0 39.5 10.5 40.5 66-67 31.739954325318834 36.0 31.0 39.0 7.0 39.5 68-69 31.19232882709133 36.0 30.5 38.0 2.0 39.0 70-71 30.29510604362973 35.0 29.5 37.0 2.0 38.0 72-73 29.703383967329746 34.5 29.5 36.5 2.0 37.0 74-75 29.318117113311168 34.0 29.0 35.5 2.0 37.0 76-77 28.908237361399383 34.0 29.0 35.0 2.0 36.5 78-79 28.358818703353357 34.0 28.5 35.0 2.0 36.0 80-81 27.70030582444024 34.0 26.0 35.0 2.0 36.0 82-83 27.085841308034702 33.5 25.5 35.0 2.0 35.0 84-85 26.58223279393127 33.0 25.0 35.0 2.0 35.0 86-87 26.55312283795284 33.0 25.0 35.0 2.0 35.0 88-89 25.941219869098568 33.0 24.0 35.0 2.0 35.0 90-91 25.328916634522955 32.5 20.0 35.0 2.0 35.0 92-93 24.821262604926254 32.0 16.5 35.0 2.0 35.0 94-95 24.10532345756112 32.0 4.5 35.0 2.0 35.0 96-97 23.32415018153543 31.0 2.0 34.0 2.0 35.0 98-99 22.757146618175582 31.0 2.0 34.0 2.0 35.0 100-101 20.426200337968147 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24258312343496513 1101 2 0.24622340259654507 1101 3 0.4770401842056806 1101 4 0.32808528616244104 1101 5 0.3345419281160744 1101 6 0.03237281227083599 1101 7 0.3191754626685963 1101 8 0.32320963201761543 1101 9 0.34150060062496834 1101 10-11 0.3689418232460753 1101 12-13 0.3567684821514163 1101 14-15 0.5581868916642421 1101 16-17 0.41611160892021104 1101 18-19 0.6219425343045017 1101 20-21 0.6821783978229945 1101 22-23 0.6361049996903532 1101 24-25 0.5130652963079214 1101 26-27 0.6787604299061556 1101 28-29 0.7676241720863999 1101 30-31 0.7332836692873386 1101 32-33 0.5659598483504666 1101 34-35 0.6913437886579956 1101 36-37 1.0054575070568106 1101 38-39 0.9366199917970661 1101 40-41 0.9530166413380243 1101 42-43 0.9676552208501832 1101 44-45 0.9795101700610758 1101 46-47 1.0726359154488918 1101 48-49 1.3732618313669533 1101 50-51 1.2980648568784972 1101 52-53 1.3031095724686637 1101 54-55 1.1850353771364794 1101 56-57 1.4040562624398945 1101 58-59 1.321670859066387 1101 60-61 1.0282500039407338 1101 62-63 0.9352609794732913 1101 64-65 1.4135758992139813 1101 66-67 1.2265511343045254 1101 68-69 1.117714729971084 1101 70-71 1.0856899916570626 1101 72-73 0.9843050386087349 1101 74-75 0.8389188638813927 1101 76-77 0.8968217149833251 1101 78-79 0.38638491640317696 1101 80-81 0.5651869438020753 1101 82-83 0.688735974375053 1101 84-85 0.6892074273637263 1101 86-87 0.3265181020883112 1101 88-89 0.4919207281681288 1101 90-91 0.42039463017112766 1101 92-93 0.308473697207031 1101 94-95 0.47463909697525253 1101 96-97 0.31024975454745274 1101 98-99 0.059099170270137336 1101 100-101 0.4068898861114896 1102 1 0.3846463513883762 1102 2 0.35686954790991265 1102 3 0.3342032457697428 1102 4 0.14998489792061775 1102 5 0.32432562195966597 1102 6 0.13543714948659158 1102 7 0.37185987753271377 1102 8 0.19781584388672258 1102 9 0.5681894525384479 1102 10-11 0.4185826995633235 1102 12-13 0.5136819596888529 1102 14-15 0.41566193326158185 1102 16-17 0.48163573537610915 1102 18-19 0.48900853541376677 1102 20-21 0.5353869391430237 1102 22-23 0.4768842564845883 1102 24-25 0.6603684022424758 1102 26-27 0.3211356378928443 1102 28-29 0.41643032849239603 1102 30-31 0.41866636479482366 1102 32-33 0.2862219115784157 1102 34-35 0.32237817579055417 1102 36-37 0.46107037449996113 1102 38-39 0.7129538797615709 1102 40-41 0.42858929802132195 1102 42-43 0.6276011443110789 1102 44-45 0.7876535422185924 1102 46-47 0.5915141739070151 1102 48-49 0.5153749761256847 1102 50-51 0.2544597542717355 1102 52-53 0.48104495793732127 1102 54-55 0.536693724335592 1102 56-57 0.5911486085297426 1102 58-59 0.6190044697152999 1102 60-61 0.39797546151144303 1102 62-63 0.393601245696253 1102 64-65 0.25208116821009696 1102 66-67 0.6674316112873306 1102 68-69 0.3425677527109663 1102 70-71 -0.08223568776611856 1102 72-73 0.06464475020052873 1102 74-75 0.026611598213062138 1102 76-77 0.3654600954603069 1102 78-79 0.23112562633107459 1102 80-81 0.3241872211176613 1102 82-83 0.18231334542330657 1102 84-85 -0.19326068134398255 1102 86-87 -0.4512841718994878 1102 88-89 -0.5421960216932149 1102 90-91 -0.7431993798122356 1102 92-93 -0.5637808785001255 1102 94-95 -0.3942031104568038 1102 96-97 -0.698475980338845 1102 98-99 -0.3359909572950315 1102 100-101 0.15159593159124896 1103 1 0.30773809896514237 1103 2 0.3383577147227008 1103 3 0.2910940178109023 1103 4 0.3174844161243797 1103 5 0.15212757248801267 1103 6 0.3541373582197238 1103 7 0.2761466426577215 1103 8 0.24702420515519208 1103 9 0.44283828414862114 1103 10-11 0.3646093055734312 1103 12-13 0.4219642407158517 1103 14-15 0.5426118508812081 1103 16-17 0.5856711521502191 1103 18-19 0.4806483526840566 1103 20-21 0.5592066740600785 1103 22-23 0.5871789529258109 1103 24-25 0.7681699727625215 1103 26-27 0.6402245408360088 1103 28-29 0.6723917087911389 1103 30-31 0.856277144001858 1103 32-33 0.9002298320372475 1103 34-35 0.6942339463523908 1103 36-37 0.7912704488730142 1103 38-39 0.7685884529172426 1103 40-41 0.592737957270586 1103 42-43 0.30233303487954544 1103 44-45 0.43795089871795057 1103 46-47 0.6656144363841818 1103 48-49 0.6070791233734738 1103 50-51 0.414399821533209 1103 52-53 0.8703349667046538 1103 54-55 0.5259924190070606 1103 56-57 0.44932950876941646 1103 58-59 0.32912191942527613 1103 60-61 0.40617006919358545 1103 62-63 0.15387843461195416 1103 64-65 -0.05258777669683923 1103 66-67 -0.07263456444479388 1103 68-69 -0.020496254259469993 1103 70-71 0.05305812651296549 1103 72-73 -0.15404052963487658 1103 74-75 -0.2337673786417156 1103 76-77 -0.19865680595196977 1103 78-79 -0.12102264205250535 1103 80-81 -0.336005280232726 1103 82-83 -0.24548914797405175 1103 84-85 0.052627764503476726 1103 86-87 -0.09757515511669013 1103 88-89 0.009776900000645128 1103 90-91 0.22600637568499238 1103 92-93 -0.11799449202625212 1103 94-95 -0.023344861697935926 1103 96-97 -0.01874562337532737 1103 98-99 0.3864414758710062 1103 100-101 0.6636408377156222 1104 1 0.1595289362428467 1104 2 0.18298038125007565 1104 3 0.07210586565067345 1104 4 0.17152535184388285 1104 5 -0.02384887648162959 1104 6 0.22527100603110028 1104 7 0.04819024099371916 1104 8 0.06991734958740636 1104 9 0.32370092903219216 1104 10-11 0.09309206955149563 1104 12-13 0.06817242303812066 1104 14-15 0.3259742479860819 1104 16-17 0.2985746631073951 1104 18-19 0.29757471821255166 1104 20-21 0.282350811616098 1104 22-23 0.28269777473141033 1104 24-25 0.3074207971289411 1104 26-27 0.22162988803422934 1104 28-29 0.11555931165947442 1104 30-31 0.06453982692281102 1104 32-33 0.18894424900727813 1104 34-35 0.1490859889863998 1104 36-37 0.0340453559894911 1104 38-39 0.32691555829953245 1104 40-41 0.09730646071404436 1104 42-43 0.1304589975332675 1104 44-45 0.32638619633365096 1104 46-47 0.027664651081074965 1104 48-49 0.2528841630582477 1104 50-51 0.3886067287996866 1104 52-53 0.21928773338820662 1104 54-55 0.265692191586389 1104 56-57 0.20123606539555539 1104 58-59 0.15874392967393902 1104 60-61 0.11173933070592312 1104 62-63 0.3255113899823243 1104 64-65 -0.06593970012920636 1104 66-67 -0.29771405452962796 1104 68-69 0.320062841629543 1104 70-71 0.4759855581595289 1104 72-73 0.08210060511120076 1104 74-75 -0.027698541701528967 1104 76-77 0.3596542272985914 1104 78-79 0.5115121742028457 1104 80-81 0.41893489125692085 1104 82-83 0.4650249727330156 1104 84-85 0.634576885491807 1104 86-87 0.6916535700686062 1104 88-89 0.519773765934957 1104 90-91 0.5281450406801582 1104 92-93 0.8076116282415136 1104 94-95 0.8365972250540707 1104 96-97 0.7565592783569777 1104 98-99 0.7774201883325347 1104 100-101 0.8914793770802909 1105 1 -0.09872835197487362 1105 2 0.0019337851102072534 1105 3 -0.07615653710579195 1105 4 -0.051231653728272875 1105 5 -0.12840889155605595 1105 6 -0.1472719964723268 1105 7 -0.12568792530954198 1105 8 -0.1679652313703599 1105 9 -0.18407316986683497 1105 10-11 -0.18787511664463352 1105 12-13 -0.22757578014366686 1105 14-15 0.062162301500308104 1105 16-17 -0.15224904681749507 1105 18-19 -0.22630883181571448 1105 20-21 -0.23211121966333792 1105 22-23 -0.2235944538615584 1105 24-25 0.01190409412213711 1105 26-27 -0.017673996323360086 1105 28-29 0.02225531962739069 1105 30-31 -0.0845032159585628 1105 32-33 -0.09178605328887812 1105 34-35 -0.24715347910156993 1105 36-37 -0.1261271923967371 1105 38-39 0.04525387157848826 1105 40-41 0.14021609488993647 1105 42-43 -0.10976281193670445 1105 44-45 7.260170556122603E-4 1105 46-47 0.1651905602576349 1105 48-49 -0.0913283325674783 1105 50-51 0.10943097709707672 1105 52-53 -0.06428113791385215 1105 54-55 -0.11665861193456095 1105 56-57 -0.0804929178879803 1105 58-59 -0.2562389769991924 1105 60-61 -0.0331434386822167 1105 62-63 -0.3727308238053979 1105 64-65 -0.16770825379148846 1105 66-67 -0.36411826460257757 1105 68-69 -0.3243071279524088 1105 70-71 -0.2532171121680733 1105 72-73 -0.06595452423279369 1105 74-75 -0.04873140934265052 1105 76-77 -0.025282110153291626 1105 78-79 -0.0728090726678623 1105 80-81 0.11451754489496935 1105 82-83 0.09758843339144718 1105 84-85 0.1783331104238357 1105 86-87 0.07226946820852831 1105 88-89 -0.016344299154276598 1105 90-91 0.035640531810468445 1105 92-93 0.015440910321782297 1105 94-95 -0.054445602478295996 1105 96-97 0.4078863665693113 1105 98-99 0.5894407003247828 1105 100-101 0.767026498133351 1106 1 -0.10585138135736827 1106 2 -0.16758383914999087 1106 3 -0.011729783845435549 1106 4 -0.1048167372667379 1106 5 -0.2862887428104415 1106 6 -0.47324486611042005 1106 7 -0.26828992604279733 1106 8 -0.1790321145212559 1106 9 -0.30028853675521816 1106 10-11 0.020999863714656897 1106 12-13 -0.32465324308819987 1106 14-15 -0.3261501690386339 1106 16-17 -0.09286602686254497 1106 18-19 -0.2178764220320275 1106 20-21 -0.10658092060086233 1106 22-23 -0.014826842695164544 1106 24-25 -0.11831116801582908 1106 26-27 -0.25373957009384895 1106 28-29 -0.22479595570912636 1106 30-31 -0.05379300802896125 1106 32-33 -0.08289797912556907 1106 34-35 -0.02583938493093285 1106 36-37 0.05802406722720832 1106 38-39 -0.05859832556182454 1106 40-41 0.018988419825774372 1106 42-43 0.12550665854985965 1106 44-45 0.1561049517437212 1106 46-47 0.02230311459691592 1106 48-49 0.007702725935637034 1106 50-51 -0.14741970116196512 1106 52-53 -0.2601199269988541 1106 54-55 -0.34609348335340684 1106 56-57 0.06780226882966645 1106 58-59 -0.15574926872914574 1106 60-61 -0.15013657752891874 1106 62-63 -0.10623263075035538 1106 64-65 0.03974735821290665 1106 66-67 -0.2292023268243355 1106 68-69 -0.06271125511943154 1106 70-71 -0.10281906052610879 1106 72-73 -0.11491487619424845 1106 74-75 -0.1692494002293614 1106 76-77 -0.04815698576215155 1106 78-79 0.28481675878868984 1106 80-81 -0.07596587834914814 1106 82-83 -0.5155132269029004 1106 84-85 -0.33067542673638073 1106 86-87 0.18843769740247396 1106 88-89 0.10915981230018446 1106 90-91 0.18665661100179776 1106 92-93 0.40935229145623353 1106 94-95 0.38114335682615774 1106 96-97 0.3552204680724813 1106 98-99 0.9517855387472416 1106 100-101 0.8841689063457814 1107 1 0.11621369668337067 1107 2 0.16653125822287507 1107 3 -0.057450261654189205 1107 4 -0.047827665005286235 1107 5 -0.13666826296672951 1107 6 -0.08806243189052054 1107 7 0.027094783263073907 1107 8 0.23542831566942368 1107 9 0.08150060062497033 1107 10-11 0.11314033704650228 1107 12-13 0.08692771782020259 1107 14-15 0.1419289298807982 1107 16-17 0.2244555579647951 1107 18-19 0.19118881880556415 1107 20-21 0.0807346610926345 1107 22-23 -0.03724956506547983 1107 24-25 0.09398355533127756 1107 26-27 -0.0077204618135979786 1107 28-29 -0.22742359861423722 1107 30-31 -0.015729069566162934 1107 32-33 0.16081653624855363 1107 34-35 0.22644994577051847 1107 36-37 0.20458701873409524 1107 38-39 0.3755478049605543 1107 40-41 0.2904954099155077 1107 42-43 0.27270299155081545 1107 44-45 0.30186155010353843 1107 46-47 0.27117625515165145 1107 48-49 0.3708414492013432 1107 50-51 0.36767207556215453 1107 52-53 0.22555649391239996 1107 54-55 0.22676573807066092 1107 56-57 -0.1040806802352705 1107 58-59 0.15531735588167095 1107 60-61 0.13532006763499993 1107 62-63 0.23135970558793417 1107 64-65 0.16654829836471663 1107 66-67 0.2793165270858431 1107 68-69 0.23845782975877228 1107 70-71 0.17907640354665944 1107 72-73 -0.030095173705490197 1107 74-75 -0.18577328049228115 1107 76-77 -0.06616129987867225 1107 78-79 -0.37349300291741727 1107 80-81 -0.3281941602318952 1107 82-83 0.054054445712633736 1107 84-85 -0.18766093781674087 1107 86-87 -0.1493683098012859 1107 88-89 -0.04727778563229634 1107 90-91 0.08845195501189096 1107 92-93 0.15114885644269904 1107 94-95 0.0671921755315168 1107 96-97 0.42914041272155146 1107 98-99 0.20547390487735484 1107 100-101 0.506274174858838 1108 1 -0.006076352682139685 1108 2 -0.09981922709015123 1108 3 -0.2986223735755189 1108 4 -0.3019275541046369 1108 5 -0.12441030609039672 1108 6 0.0063969519729454305 1108 7 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54-55 -2.6179578201424363 2316 56-57 -2.6228314926621508 2316 58-59 -2.569264515083276 2316 60-61 -2.1640289076238943 2316 62-63 -2.533531537533513 2316 64-65 -2.9712880463642506 2316 66-67 -2.777071314675066 2316 68-69 -2.666026766627553 2316 70-71 -2.6546501443973582 2316 72-73 -2.801596321935481 2316 74-75 -3.0869995034655453 2316 76-77 -2.932463999302392 2316 78-79 -2.9264892332566887 2316 80-81 -2.9722450289870395 2316 82-83 -2.790311644672567 2316 84-85 -2.836710939983213 2316 86-87 -2.7775465237620303 2316 88-89 -3.078912605165204 2316 90-91 -3.33157815894452 2316 92-93 -3.579111336806573 2316 94-95 -3.924017365609778 2316 96-97 -3.7971822182416624 2316 98-99 -4.045169537212853 2316 100-101 -4.089402630895311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5779.0 3 1121.0 4 960.0 5 932.0 6 949.0 7 1037.0 8 1285.0 9 1606.0 10 1960.0 11 2541.0 12 3469.0 13 4402.0 14 4597.0 15 4786.0 16 5489.0 17 6540.0 18 5669.0 19 5225.0 20 5728.0 21 6118.0 22 6601.0 23 7376.0 24 8011.0 25 9343.0 26 10617.0 27 12631.0 28 14803.0 29 17209.0 30 20465.0 31 24384.0 32 29268.0 33 35836.0 34 45943.0 35 64361.0 36 90810.0 37 117366.0 38 66292.0 39 1807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.366893219658294 17.654425447511134 14.091657393015792 13.887023939814771 2 13.346160975677012 29.684093774559294 27.09354323278035 29.87620201698335 3 37.44401007359114 21.263457496675837 30.03526369646873 11.257268733264297 4 11.90782972399476 14.613493078802827 44.784703656322534 28.693973540879874 5 9.018805011400692 44.267321695638316 33.52508152107289 13.1887917718881 6 24.91306617994956 36.96115195158961 12.378824743562458 25.74695712489837 7 20.754078479531806 35.76567002826685 23.254606760154473 20.22564473204688 8 22.653644373799832 16.986558127141926 19.946587773377804 40.41320972568044 9 14.946334202623799 29.0397122306565 31.495674731211608 24.51827883550809 10-11 27.461911094879444 20.895513246034536 23.90847790617638 27.73409775290964 12-13 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21.072152522451816 36.682919554738 19.842923767216007 38-39 21.0837863968729 28.26109679035801 31.168158433130284 19.48695837963881 40-41 20.25523047274806 30.77319096489044 27.947106681051043 21.024471881310454 42-43 17.944021631359938 30.67296710256296 20.87967325455739 30.503338011519716 44-45 23.058167725114185 21.243433705211988 25.581146874389876 30.117251695283954 46-47 29.319458653829155 29.134290407454692 27.037869173906724 14.508381764809425 48-49 24.27044671109075 25.559196061488503 28.822889984286544 21.347467243134204 50-51 11.815896017962714 28.850572327058526 38.67710238239642 20.65642927258234 52-53 20.959804063258172 33.60690268044661 22.186945545452588 23.24634771084263 54-55 16.073068907971273 23.856318927765123 29.09211943341156 30.978492730852043 56-57 25.060119293278397 21.017658817534773 26.507824154237404 27.414397734949425 58-59 33.00671574126723 21.32209432773432 24.720843386454796 20.950346544543653 60-61 22.536860194370163 25.626871049047317 35.001302100337774 16.83496665624474 62-63 17.29394331144885 26.6801888286139 32.90673704954522 23.11913081039203 64-65 16.50195972304016 35.64904581707919 26.438347528319493 21.41064693156115 66-67 22.22670707648496 24.885789982732028 21.922480191039416 30.965022749743593 68-69 17.788043449009233 25.393849875322843 27.807504311607545 29.01060236406038 70-71 28.458500151087485 25.213540821685243 23.043801530250942 23.28415749697633 72-73 23.638055039805643 18.905200681174293 36.09231987514367 21.364424403876388 74-75 17.499135114357102 35.167710935998464 25.818181818181817 21.514972131462617 76-77 20.592623452970535 24.17497526828245 25.484622521351525 29.747778757395494 78-79 22.1529211232926 25.653027943985997 29.00788936142743 23.186161571293976 80-81 27.61493928236295 22.954194900502387 25.999721792486397 23.431144024648262 82-83 22.310355826617627 21.253982345096865 33.96036293623252 22.47529889205299 84-85 21.29351153633606 26.93797302667127 29.951662602041562 21.816852834951113 86-87 21.020537894679396 28.634524785908262 27.51950289055552 22.825434428856823 88-89 19.79857413674081 28.75765090796156 21.91230802582359 29.531466929474043 90-91 23.747901252790644 21.31996592803065 25.26668696224408 29.66544585693463 92-93 28.51120032350169 26.62661846942494 26.19625666722531 18.66592453984806 94-95 24.791612529234214 24.208927179323165 27.501995076504844 23.49746521493778 96-97 16.99524337074108 25.758681213582417 34.09919651636682 23.146878899309687 98-99 23.60520861521809 29.576527097837584 22.943906984181652 23.874357302762675 100-101 20.970850203051576 23.06216998833885 26.609096310941418 29.35788349766815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3125.0 1 1855.5 2 673.5 3 755.0 4 577.5 5 510.0 6 790.0 7 1303.0 8 1728.5 9 1681.5 10 1235.0 11 838.0 12 738.0 13 734.5 14 762.0 15 802.0 16 1168.0 17 1375.5 18 1043.5 19 860.0 20 902.5 21 991.0 22 1064.0 23 1158.0 24 1352.0 25 1533.5 26 1750.0 27 2048.0 28 2263.0 29 2454.0 30 2675.5 31 2777.0 32 2920.0 33 3035.5 34 2952.0 35 2913.0 36 2900.0 37 2883.0 38 2850.0 39 2781.5 40 2673.5 41 2649.5 42 2818.5 43 3109.0 44 3918.5 45 6946.0 46 23299.0 47 75305.5 48 161852.0 49 161619.5 50 75150.0 51 25863.5 52 11327.0 53 7090.0 54 5000.5 55 3542.5 56 2626.0 57 2044.5 58 1696.0 59 1532.5 60 1363.0 61 1172.0 62 1005.0 63 814.5 64 607.0 65 510.0 66 447.0 67 364.0 68 309.5 69 262.0 70 216.5 71 172.5 72 138.0 73 127.5 74 105.0 75 89.0 76 80.0 77 64.0 78 50.0 79 40.0 80 32.0 81 22.5 82 15.0 83 13.5 84 11.0 85 5.0 86 3.0 87 3.5 88 4.0 89 3.0 90 1.0 91 1.0 92 0.5 93 1.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14235071542714398 2 0.08556349454169192 3 0.0795939484108762 4 0.09352288938277954 5 0.11081926663360456 6 0.035664211499488764 7 0.038878582493004916 8 0.04316441048435979 9 0.044848128623820635 10-11 0.04155722498760171 12-13 0.046837977334092534 14-15 0.04400626955409021 16-17 0.04262868198544043 18-19 0.04676144469138977 20-21 0.04928702190058104 22-23 0.06114958151950971 24-25 0.07630304477465728 26-27 0.07660917534546835 28-29 0.06864978050438074 30-31 0.058164808454101845 32-33 0.04706757526220083 34-35 0.27735429715482246 36-37 0.43891470590036064 38-39 0.4201642084381831 40-41 0.39950039490843636 42-43 0.4253684281419711 44-45 0.4339400841246809 46-47 0.4326390291987338 48-49 0.44664450281333995 50-51 0.43738405304630534 52-53 0.4441954582468514 54-55 0.43799631418792745 56-57 0.418557022941425 58-59 0.3988881337668142 60-61 0.37416809017382097 62-63 0.4253684281419711 64-65 0.45445083236902206 66-67 0.45598148522307735 68-69 0.46478273913389534 70-71 0.4638643474214622 72-73 0.4543742997263193 74-75 0.45016500437766716 76-77 0.4340166167673836 78-79 0.4122048135970955 80-81 0.4167202395165586 82-83 0.42682254835332367 84-85 0.4262102872117015 86-87 0.4340166167673836 88-89 0.444348523532257 90-91 0.44817515566739524 92-93 0.4489404820944229 94-95 0.4538385712273999 96-97 0.453149777443075 98-99 0.4487874168090174 100-101 0.4386085753295496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 653316.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.615359932610973 #Duplication Level Percentage of deduplicated Percentage of total 1 84.31967204039226 13.166820283104391 2 7.704930883781451 2.4063053801227547 3 2.550594453775372 1.1948535131347118 4 1.3336950026256282 0.833045100252949 5 0.8060533427729281 0.629340653614176 6 0.5373560438241846 0.5034604821767111 7 0.3875344530350178 0.42360429793005283 8 0.3056327326943446 0.3818052102567735 9 0.2477023346443809 0.3481165000458055 >10 1.3933256240673655 4.192526694195959 >50 0.18477795012464399 2.0397256092967297 >100 0.1677983547077848 5.309712390495326 >500 0.01697959541685918 1.6873980246965379 >1k 0.021973594068876584 7.581671631252742 >5k 0.006991598112824367 8.343566385910526 >10k+ 0.014981995956052215 50.95804784351385 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 66352 10.156187817227803 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 43599 6.6734933783957535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 29861 4.570682487494566 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21550 3.2985569004891966 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 19118 2.9263021263829447 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 17488 2.6768057111719292 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17454 2.671601491468141 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 16825 2.575323426948062 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 16691 2.554812678703721 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 16116 2.46680013959554 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 15532 2.3774100129187103 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12188 1.865559698522614 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11968 1.831885335733397 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11960 1.8306608134501527 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10023 1.5341733556196389 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 9980 1.527591548347201 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9636 1.4749370901676984 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8277 1.2669213673015816 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6977 1.067936496274391 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6656 1.0188025396592155 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6259 0.9580356213532195 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5711 0.8741558449509884 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4331 0.6629257510913555 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3820 0.584709390249129 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3292 0.5038909195550086 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3268 0.5002173527052759 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3206 0.49072730501013295 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2941 0.45016500437766716 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2533 0.3877143679322105 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2503 0.38312240937004455 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2431 0.37210170882084626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.3696526642543578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 0.29296695626618663 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1897 0.2903648464142926 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.2660274660348132 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1613 0.24689430535912177 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1569 0.2401594328012784 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1497 0.22913873225208015 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1488 0.22776114468343037 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1461 0.22362838197748106 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1303 0.1994440668834071 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 1191 0.1823007549179876 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1151 0.17617814350176636 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 1049 0.1605654843904022 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 855 0.13087081902172915 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 735 0.11250298477306542 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 733 0.11219685420225435 No Hit GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 730 0.11173765834603774 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 719 0.11005394020657691 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 707 0.10821715678171054 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 664 0.10163534950927271 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5306528540553116E-4 0.0 10-11 0.0 0.0 0.0 3.826632135138279E-4 0.0 12-13 0.0 0.0 0.0 6.887937843248902E-4 0.0 14-15 0.0 0.0 0.0 9.949243551359527E-4 0.0 16-17 0.0 0.0 0.0 0.001683718139460843 0.0 18-19 0.0 0.0 0.0 0.0025255772091912643 0.0 20-21 0.0 0.0 0.0 0.004745023847571466 0.0 22-23 0.0 0.0 0.0 0.009719645623251229 0.0 24-25 0.0 0.0 0.0 0.018138236320555442 0.0 26-27 0.0 0.0 0.0 0.030459991795700702 0.0 28-29 0.0 0.0 0.0 0.08900746346331637 0.0 30-31 0.0 0.0 0.0 0.21145969178774132 0.0 32-33 0.0 0.0 0.0 0.49807443870959844 0.0 34-35 0.0 0.0 0.0 0.8459918324363708 0.0 36-37 0.0 0.0 0.0 1.2335531350831757 0.0 38-39 0.0 0.0 0.0 1.7963741895193137 0.0 40-41 0.0 0.0 0.0 2.339909017994355 0.0 42-43 0.0 0.0 0.0 2.919414188539696 0.0 44-45 0.0 0.0 0.0 3.5514972846218367 0.0 46-47 0.0 0.0 0.0 4.3338139583295066 0.0 48-49 0.0 0.0 0.0 4.871302708031029 1.5306528540553116E-4 50-51 0.0 0.0 0.0 5.49397228906073 1.5306528540553116E-4 52-53 0.0 0.0 0.0 6.072406002608233 1.5306528540553116E-4 54-55 0.0 0.0 0.0 6.748878031457977 1.5306528540553116E-4 56-57 0.0 0.0 0.0 7.801661064477221 1.5306528540553116E-4 58-59 0.0 0.0 0.0 8.46971450262966 1.5306528540553116E-4 60-61 0.0 0.0 0.0 9.25807725511085 1.5306528540553116E-4 62-63 0.0 0.0 0.0 10.041465385816359 1.5306528540553116E-4 64-65 0.0 0.0 0.0 10.813909348615372 1.5306528540553116E-4 66-67 0.0 0.0 0.0 11.81809415351836 1.5306528540553116E-4 68-69 0.0 0.0 0.0 12.997691775496085 1.5306528540553116E-4 70-71 0.0 0.0 0.0 14.084991030374276 1.5306528540553116E-4 72-73 0.0 0.0 0.0 14.918814172620905 1.5306528540553116E-4 74-75 0.0 0.0 0.0 16.14402524964948 1.5306528540553116E-4 76-77 0.0 0.0 0.0 17.011752352613435 1.5306528540553116E-4 78-79 0.0 0.0 0.0 18.7680234373565 1.5306528540553116E-4 80-81 0.0 0.0 0.0 20.311763373313987 1.5306528540553116E-4 82-83 0.0 0.0 0.0 21.368526103753773 1.5306528540553116E-4 84-85 0.0 0.0 0.0 22.665371734352135 1.5306528540553116E-4 86-87 0.0 0.0 0.0 23.801575347917392 1.5306528540553116E-4 88-89 0.0 0.0 0.0 25.202505372591517 1.5306528540553116E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATCAT 25 0.0050808266 56.82769 9 GGGACTC 50 0.0016395082 37.885124 7 TGAGGCC 55 0.0026105102 34.459496 5 GTACCCA 195 0.0 34.033356 1 ACCCATG 195 0.0 34.017708 3 GTCACAA 30 0.003929606 31.702042 46-47 TACCCAT 210 0.0 31.587873 2 TAGGCAG 120 1.5373553E-7 31.587873 4 GCTTTGC 620 0.0 30.58297 1 ATGGGAG 155 1.3915269E-9 30.56891 5 CTTTGCG 625 0.0 29.566248 2 TTTGCGT 630 0.0 29.331596 3 GCCTTGG 65 0.0059005236 29.171448 1 TGGGGAC 100 5.055724E-5 28.411669 6 TTGCGTT 655 0.0 28.21207 4 ATGGGGA 220 1.8189894E-12 27.99834 5 GTAAGCG 180 0.0 27.74035 94-95 GTAGGCA 140 5.9272315E-7 27.075321 3 CATGGGG 385 0.0 27.07532 4 AGTAGGC 145 8.0519567E-7 26.141687 2 >>END_MODULE