##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938744_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 653098 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80442445084811 31.0 31.0 34.0 30.0 34.0 2 31.920627532162094 31.0 31.0 34.0 30.0 34.0 3 32.05197841671541 33.0 31.0 34.0 30.0 34.0 4 35.57975372761821 37.0 35.0 37.0 33.0 37.0 5 35.50467311184539 37.0 35.0 37.0 33.0 37.0 6 35.654431647317864 37.0 35.0 37.0 33.0 37.0 7 35.60682929667523 37.0 35.0 37.0 33.0 37.0 8 35.67414691210201 37.0 35.0 37.0 33.0 37.0 9 37.15254984703674 39.0 37.0 39.0 33.0 39.0 10-11 37.020496923891976 39.0 37.0 39.0 32.5 39.0 12-13 37.06342769385299 39.0 37.0 39.0 33.0 39.0 14-15 37.86178261149169 39.0 37.0 41.0 32.5 41.0 16-17 37.93490257204891 39.5 37.0 41.0 32.5 41.0 18-19 38.273591405883955 40.0 37.0 41.0 33.0 41.0 20-21 38.251048847186794 40.0 37.5 41.0 33.0 41.0 22-23 38.20464080428971 40.0 37.5 41.0 33.0 41.0 24-25 37.95541021408732 40.0 37.0 41.0 32.5 41.0 26-27 37.67065509311007 39.5 36.5 41.0 32.5 41.0 28-29 37.62420341204536 39.5 36.5 41.0 32.5 41.0 30-31 37.67655466714031 40.0 37.0 41.0 33.0 41.0 32-33 37.41674067291585 39.5 36.0 41.0 32.0 41.0 34-35 37.133854337327634 39.0 35.5 41.0 31.0 41.0 36-37 36.95696204857464 39.0 35.5 41.0 31.0 41.0 38-39 36.779191637395925 39.0 35.0 40.5 30.5 41.0 40-41 37.01291536645343 39.0 36.0 41.0 31.0 41.0 42-43 36.69095909036622 39.0 35.0 41.0 30.5 41.0 44-45 36.47626313355729 39.0 35.0 41.0 30.0 41.0 46-47 36.23427341685321 39.0 35.0 40.5 30.0 41.0 48-49 35.84517101568218 39.0 35.0 40.0 28.0 41.0 50-51 35.57935945294581 38.5 35.0 40.0 28.0 41.0 52-53 35.63301832190575 39.0 35.0 40.0 28.0 41.0 54-55 35.53472679444739 39.0 35.0 40.0 27.5 41.0 56-57 34.961625207855484 38.0 34.0 40.0 26.5 41.0 58-59 34.63978453463339 38.0 34.0 40.0 26.0 41.0 60-61 33.77384251674327 38.0 32.5 39.5 22.0 41.0 62-63 33.62773274454982 37.0 32.5 39.5 22.5 41.0 64-65 33.242034120453596 36.5 32.5 39.0 22.0 40.5 66-67 32.376931945894796 36.0 31.5 38.5 20.5 39.0 68-69 31.88169845873054 35.5 31.5 37.0 19.5 39.0 70-71 31.058463660890098 34.5 30.5 37.0 16.0 38.0 72-73 30.396378491436202 34.0 29.5 36.0 12.5 37.0 74-75 30.054068455270112 34.0 30.0 35.5 9.5 37.0 76-77 28.21239614881687 31.5 27.5 34.0 7.0 35.0 78-79 29.199166128207406 34.0 29.0 35.0 6.0 36.0 80-81 29.079078484392845 34.0 29.0 35.0 2.0 36.0 82-83 28.75308299826366 33.5 29.0 35.0 2.0 35.0 84-85 28.36909927759693 33.0 29.0 35.0 2.0 35.0 86-87 28.20502053290624 33.5 29.0 35.0 2.0 35.0 88-89 27.527942207754425 33.0 26.5 35.0 2.0 35.0 90-91 26.92178738872267 33.0 25.0 34.0 2.0 35.0 92-93 26.760978444276358 33.0 25.0 34.0 2.0 35.0 94-95 26.323887839191055 32.0 25.0 34.0 2.0 35.0 96-97 25.61277939911009 32.0 23.5 34.0 2.0 35.0 98-99 24.885687140367907 32.0 18.0 34.0 2.0 35.0 100-101 21.138407252816577 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22786565299194095 1101 2 0.24731335977260827 1101 3 0.22080914015015196 1101 4 0.14240825652237987 1101 5 0.06425507112228246 1101 6 0.14839293755139238 1101 7 0.31011656964533785 1101 8 0.31516030733507705 1101 9 0.2867610988313274 1101 10-11 0.29832254770607136 1101 12-13 0.24028927025883462 1101 14-15 0.3275447607620947 1101 16-17 0.37367169719478 1101 18-19 0.45184247748905193 1101 20-21 0.4972578515891328 1101 22-23 0.15062471945618228 1101 24-25 0.25258029358711553 1101 26-27 0.3826147333355365 1101 28-29 0.40698224177686626 1101 30-31 0.5203748455710198 1101 32-33 0.4675546880525516 1101 34-35 0.4752975544039941 1101 36-37 0.5730870214547394 1101 38-39 0.7489614873021893 1101 40-41 0.5509101169603525 1101 42-43 0.6007286285703657 1101 44-45 0.5878603101379838 1101 46-47 0.6048076450103892 1101 48-49 0.841069441643036 1101 50-51 0.7689456840802507 1101 52-53 0.5553536385570652 1101 54-55 0.6647999202467076 1101 56-57 0.6817256219471162 1101 58-59 0.6531035587945055 1101 60-61 0.6759964000407663 1101 62-63 0.8635617314559809 1101 64-65 0.8186704265380627 1101 66-67 0.8393795025559143 1101 68-69 0.7885688944811129 1101 70-71 0.7776544672307288 1101 72-73 1.0013686643170239 1101 74-75 0.8432060720982157 1101 76-77 0.8607791783453749 1101 78-79 1.0244501704127948 1101 80-81 1.070049467448083 1101 82-83 0.9438797466297402 1101 84-85 0.8897701245326424 1101 86-87 0.9237804854665548 1101 88-89 0.8956832079753028 1101 90-91 0.7584712748444389 1101 92-93 0.7060090875847003 1101 94-95 0.9045150043022225 1101 96-97 0.8354836818926685 1101 98-99 0.8114008380421112 1101 100-101 1.2023526339715964 1102 1 0.30481816148110497 1102 2 0.13286503608425093 1102 3 0.16305888906209987 1102 4 0.15587135853586886 1102 5 0.1511634199991576 1102 6 0.17681769881847487 1102 7 0.39692926593242106 1102 8 0.25183829289153437 1102 9 0.3656107170180718 1102 10-11 0.3635217442243146 1102 12-13 0.246403064956489 1102 14-15 0.3687431282912286 1102 16-17 0.6094752586595433 1102 18-19 0.6032366139853025 1102 20-21 0.483220834036878 1102 22-23 0.3354055953150663 1102 24-25 0.5772690241178253 1102 26-27 0.35495519278681087 1102 28-29 0.6192353385294638 1102 30-31 0.4542629639251601 1102 32-33 0.2625193763048159 1102 34-35 0.29770656487794867 1102 36-37 0.40532832105459704 1102 38-39 0.5141463359319758 1102 40-41 0.5129320532876136 1102 42-43 0.7204657433694166 1102 44-45 0.6746211946056064 1102 46-47 0.7593662880189882 1102 48-49 0.5480975156781369 1102 50-51 0.4363058320229243 1102 52-53 0.3225631336708119 1102 54-55 0.45758851208116624 1102 56-57 0.5238350803940435 1102 58-59 0.2468191836450515 1102 60-61 0.255507615306918 1102 62-63 0.19392584898113086 1102 64-65 0.4156382394415985 1102 66-67 0.6018023826553858 1102 68-69 0.30867985148527666 1102 70-71 0.3451453403794531 1102 72-73 0.11015355758467749 1102 74-75 0.18174051500159294 1102 76-77 -0.08739306008465064 1102 78-79 0.29121727229874494 1102 80-81 0.2275016012979414 1102 82-83 0.08389919598966245 1102 84-85 0.10939245622393656 1102 86-87 0.1801174695404022 1102 88-89 0.3269488511592584 1102 90-91 0.0893694526626021 1102 92-93 0.2504197879210821 1102 94-95 0.2122094580462246 1102 96-97 0.15715537032030014 1102 98-99 0.7012887650910642 1102 100-101 1.3332452320553543 1103 1 -0.007591257397166373 1103 2 0.019882131566490102 1103 3 -0.06359520663311713 1103 4 -0.18727200767938257 1103 5 -0.12070461596836424 1103 6 0.044760163248334095 1103 7 -0.07830799816706957 1103 8 -0.18436922164040226 1103 9 0.12200399102889037 1103 10-11 0.2060456524458374 1103 12-13 0.07494176744523173 1103 14-15 0.0118587033092723 1103 16-17 -0.1232656286848055 1103 18-19 -0.31748834666548476 1103 20-21 0.02187916853899452 1103 22-23 -0.04587534511826874 1103 24-25 0.1432003928472767 1103 26-27 0.14071882735594698 1103 28-29 0.10395277737584507 1103 30-31 0.17339785252661244 1103 32-33 0.3358504759531442 1103 34-35 0.11137437955482454 1103 36-37 0.1833156150231261 1103 38-39 0.2903168128496958 1103 40-41 0.35141195271984316 1103 42-43 0.36806686956222734 1103 44-45 0.1309591459528292 1103 46-47 0.0868374968581449 1103 48-49 -0.020049356903115267 1103 50-51 0.019614490928837824 1103 52-53 0.11847055521912608 1103 54-55 -0.06630282693507894 1103 56-57 -0.472736472848176 1103 58-59 -0.32695215973467384 1103 60-61 -0.3072511415175043 1103 62-63 -0.08475103030117737 1103 64-65 -0.0030764047012112883 1103 66-67 0.07390469581802961 1103 68-69 -0.17050012638565804 1103 70-71 0.08761184809086231 1103 72-73 0.1046693414263018 1103 74-75 0.45529809992057935 1103 76-77 0.27604826932155646 1103 78-79 0.23535956322842821 1103 80-81 0.2716324640717609 1103 82-83 0.40721990640538763 1103 84-85 0.45142027858883793 1103 86-87 0.33190026185444665 1103 88-89 0.3716615109586421 1103 90-91 0.3445121227992729 1103 92-93 0.4702754295341123 1103 94-95 0.7232545109533923 1103 96-97 0.6118531215709062 1103 98-99 0.7159505695968775 1103 100-101 1.1768457245049788 1104 1 0.04463327738206857 1104 2 0.026486999200333372 1104 3 0.13886446655393314 1104 4 0.01743280849793649 1104 5 -0.06745351067704775 1104 6 0.05769597571105578 1104 7 0.007565521177767209 1104 8 -0.20679505128089914 1104 9 0.15352047375767341 1104 10-11 0.2370565299972256 1104 12-13 0.035435442900471514 1104 14-15 0.039540266768483434 1104 16-17 -0.021139782916151262 1104 18-19 0.05331481035884167 1104 20-21 -0.18352325478181086 1104 22-23 -0.18527773259871338 1104 24-25 0.02182314994431067 1104 26-27 -0.021192590616969653 1104 28-29 -0.04701324851625799 1104 30-31 -0.11074841713118388 1104 32-33 -0.2970763252173043 1104 34-35 -0.14990573595355983 1104 36-37 -0.11327622207728893 1104 38-39 0.12100051905084541 1104 40-41 -0.024753179211103316 1104 42-43 -0.19513249759928897 1104 44-45 -0.20761584105504483 1104 46-47 0.3098068200640185 1104 48-49 0.38554583639166395 1104 50-51 0.172226613676969 1104 52-53 0.08369643913217573 1104 54-55 0.051381578153232965 1104 56-57 0.035549051995594994 1104 58-59 0.40881729454454074 1104 60-61 0.35586220876320596 1104 62-63 0.6902444256522884 1104 64-65 0.4372545042872886 1104 66-67 0.24823864099762716 1104 68-69 0.16234424194066932 1104 70-71 0.14674333359727498 1104 72-73 0.25648529602167613 1104 74-75 0.17323569160152275 1104 76-77 0.2205225807385034 1104 78-79 0.22815692947345667 1104 80-81 0.31743517309149993 1104 82-83 -0.14134507089998039 1104 84-85 -0.1932391227234298 1104 86-87 -0.14347228596044204 1104 88-89 0.11903940410826763 1104 90-91 0.15877454084643006 1104 92-93 0.31508664040091006 1104 94-95 0.2925697807417542 1104 96-97 0.5301985254278385 1104 98-99 0.9755180275676452 1104 100-101 1.6756911526821625 1105 1 0.002780393423922334 1105 2 0.1038607532658169 1105 3 -0.021526520904721735 1105 4 0.056408870030388414 1105 5 -0.19078381445847725 1105 6 -0.07001591139932373 1105 7 -0.028936823595252292 1105 8 0.04292515332652158 1105 9 -0.018242600261039854 1105 10-11 0.020493644261406985 1105 12-13 -0.0038598482745229035 1105 14-15 0.1762969937507819 1105 16-17 -0.07324181622119141 1105 18-19 0.022350786913619913 1105 20-21 -0.22852211557015067 1105 22-23 -0.03436598770201016 1105 24-25 -0.17094773831529864 1105 26-27 -0.04109374209707539 1105 28-29 -0.21008952061509234 1105 30-31 -0.10416727897109723 1105 32-33 0.14164660598682843 1105 34-35 0.07114013546824793 1105 36-37 -0.0346918991320635 1105 38-39 0.008818215139221763 1105 40-41 0.15675829177405376 1105 42-43 0.06949385349322768 1105 44-45 -0.14730272059154714 1105 46-47 -0.1367791753941603 1105 48-49 -0.1133324782761207 1105 50-51 -0.19913385542785988 1105 52-53 -0.056665704989370624 1105 54-55 -0.1914858301277107 1105 56-57 0.09230683055788091 1105 58-59 0.2164482600702371 1105 60-61 0.2087649451245852 1105 62-63 -0.1130437648539484 1105 64-65 -0.20157533755006085 1105 66-67 -0.0755052842500632 1105 68-69 -0.09133691399611976 1105 70-71 0.056629187092148925 1105 72-73 0.19087045973014938 1105 74-75 0.41097163986378504 1105 76-77 0.2947278241166771 1105 78-79 -0.14463443955851574 1105 80-81 0.07816942630695678 1105 82-83 0.37845092258022817 1105 84-85 0.2003134400020663 1105 86-87 0.20716704964129917 1105 88-89 0.37381345211539596 1105 90-91 0.3176657749256364 1105 92-93 0.453694899309923 1105 94-95 0.5742943218647945 1105 96-97 0.849386855894366 1105 98-99 0.8476158545526928 1105 100-101 1.5064631375894884 1106 1 0.1207659086697106 1106 2 0.09933516361302352 1106 3 0.07873177700523826 1106 4 0.1642870757559507 1106 5 -0.062406646942363864 1106 6 0.054564444100790865 1106 7 0.03837833569642868 1106 8 0.061416989822276946 1106 9 0.010081760744334645 1106 10-11 0.06757148488132714 1106 12-13 2.8582123318443564E-4 1106 14-15 -0.02919321076095116 1106 16-17 0.22076739266081802 1106 18-19 -0.05881951049934031 1106 20-21 -0.043481889434026755 1106 22-23 -0.15423915649885345 1106 24-25 -0.017575887291783943 1106 26-27 -0.038743732897749794 1106 28-29 0.12553050189338677 1106 30-31 -0.0672528995909687 1106 32-33 -0.2485230199949271 1106 34-35 -0.11730503536439585 1106 36-37 -0.16780492656994284 1106 38-39 -0.1550731229204203 1106 40-41 -0.057677956908776196 1106 42-43 -0.06773378079898862 1106 44-45 0.03402551846663471 1106 46-47 -0.2334592945791698 1106 48-49 0.17419658322553744 1106 50-51 0.08368801937053405 1106 52-53 0.21880746285170716 1106 54-55 -0.021859848368656287 1106 56-57 -0.2476105905728474 1106 58-59 -0.19866706600399198 1106 60-61 0.07564474938207155 1106 62-63 -0.1318008286207899 1106 64-65 -0.07995071299001921 1106 66-67 -0.32584757079586524 1106 68-69 -0.2924269741859895 1106 70-71 -0.17701333911397654 1106 72-73 -0.18550854287194696 1106 74-75 -0.10178196878023371 1106 76-77 -0.02025942824826643 1106 78-79 -0.1529759567062925 1106 80-81 -0.15628909190889928 1106 82-83 0.041044797502866714 1106 84-85 0.007033247700345413 1106 86-87 0.2036689169846504 1106 88-89 0.07014017388243232 1106 90-91 0.07147035260496892 1106 92-93 0.20759863853655958 1106 94-95 0.2566117869611233 1106 96-97 0.8672896966934864 1106 98-99 0.901817795251695 1106 100-101 1.3607828774278161 1107 1 0.01885034497774285 1107 2 0.04751464162016816 1107 3 -0.042711173425779236 1107 4 -0.0068581241121847825 1107 5 -0.23787957583995478 1107 6 -0.10151436686091841 1107 7 0.13505433117931176 1107 8 -0.13586993791096091 1107 9 0.20391772789007234 1107 10-11 -0.04724660320233909 1107 12-13 -0.0431738368972745 1107 14-15 -0.008887253645454507 1107 16-17 -0.03996249953173958 1107 18-19 -0.05718872119732055 1107 20-21 0.035686793800046246 1107 22-23 0.14408305941063304 1107 24-25 0.04684905782251292 1107 26-27 -0.23351959581325588 1107 28-29 -0.054403424836827696 1107 30-31 0.030359484587918928 1107 32-33 0.1301427488608553 1107 34-35 0.17395744105053268 1107 36-37 -0.12888342189501145 1107 38-39 0.14111679213825568 1107 40-41 -0.11016832999128212 1107 42-43 0.021857925672968292 1107 44-45 -0.03110785554875406 1107 46-47 -0.17412662057571282 1107 48-49 -0.23332538859161644 1107 50-51 -0.34702973834678374 1107 52-53 -0.4101371183686098 1107 54-55 -0.47641518648564585 1107 56-57 -0.6073521893776359 1107 58-59 -0.2988536423284387 1107 60-61 -0.3281351747248422 1107 62-63 -0.2522547842492955 1107 64-65 -0.21872562198145573 1107 66-67 -0.518796248278349 1107 68-69 -0.3151177414635846 1107 70-71 -0.18884269893273142 1107 72-73 -0.14752693396678396 1107 74-75 -0.007792391803473464 1107 76-77 -0.34638221791502133 1107 78-79 0.014756860385780612 1107 80-81 0.19103045161037713 1107 82-83 0.40468275526367137 1107 84-85 -0.04460139938282026 1107 86-87 0.2199031614557505 1107 88-89 0.41504334357570727 1107 90-91 0.2912060595258268 1107 92-93 0.20587666531657334 1107 94-95 0.6068562300027374 1107 96-97 1.025244792120965 1107 98-99 1.0166288691878265 1107 100-101 1.8879556849806534 1108 1 0.15401440531367072 1108 2 -0.04152617830378347 1108 3 0.03671266379816274 1108 4 0.08381448549220494 1108 5 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-0.9358480683500332 2316 62-63 -1.1725726845949183 2316 64-65 -1.000737670752578 2316 66-67 -1.0120098139465483 2316 68-69 -1.2608172662331718 2316 70-71 -0.925428840445786 2316 72-73 -1.263544359081994 2316 74-75 -1.4142344040922588 2316 76-77 -1.5988273744050367 2316 78-79 -1.0583668195297342 2316 80-81 -1.1399387304172741 2316 82-83 -1.3251319528776477 2316 84-85 -1.6770658771093956 2316 86-87 -1.6840486914628379 2316 88-89 -2.304978737837505 2316 90-91 -2.6090465566416086 2316 92-93 -2.5847339337617647 2316 94-95 -2.2904831997207644 2316 96-97 -2.867192323854457 2316 98-99 -2.9731524768183206 2316 100-101 -2.524315700388012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 8.0 5 30.0 6 100.0 7 154.0 8 308.0 9 543.0 10 926.0 11 1353.0 12 2045.0 13 2746.0 14 3002.0 15 3886.0 16 4867.0 17 6007.0 18 5863.0 19 5119.0 20 5370.0 21 5945.0 22 6016.0 23 6616.0 24 7276.0 25 8037.0 26 9301.0 27 10640.0 28 12972.0 29 15813.0 30 19491.0 31 23649.0 32 29197.0 33 36185.0 34 47444.0 35 67610.0 36 102478.0 37 137620.0 38 63483.0 39 996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.688375005179246 18.716037560673932 14.642156163151684 13.953431270995138 2 13.648028320405206 30.29131922008642 26.850947331028426 29.20970512847995 3 37.214782467562294 21.2104462117477 30.552229527574724 11.022541793115275 4 11.940780709786281 15.53733130403094 43.58427066075842 28.937617325424362 5 8.90740343676295 44.79707170643551 33.5257450914326 12.769779765368947 6 24.803275600968348 37.61964625592011 12.027329340913928 25.54974880219762 7 20.551892671543932 35.71638559603612 22.678526040502344 21.053195691917598 8 23.182278922918155 17.712808797454592 19.51330428205262 39.591607997574634 9 14.48817788448288 28.951704032166685 30.71545158613256 25.844666497217876 10-11 27.493117418825353 21.206924535062118 24.158242713957172 27.141715332155357 12-13 37.384818204924834 21.07424919384227 22.077773932855408 19.463158668377485 14-15 24.852319253772023 26.8102949327666 33.85977295903525 14.477612854426136 16-17 17.758237888160718 28.731472407668278 31.575151589391805 21.9351381147792 18-19 16.55218665498899 37.915136778860145 25.825297275447177 19.70737929070369 20-21 23.74283798143617 25.066835298837237 20.9004621052277 30.289864614498896 22-23 18.184407240567264 26.568294497916085 27.5117976168967 27.735500644619947 24-25 30.2232287087523 26.595972726869054 22.08446831022038 21.096330254158264 26-27 25.081458910716748 19.204091319726224 37.15422070465021 18.560229064906828 28-29 16.83468637172369 38.75406141191674 25.433166232326542 18.978085984033026 30-31 20.99202569905282 25.19935752367945 24.49862042143752 29.309996355830215 32-33 22.535745018358654 27.26757699457049 29.42812564117483 20.768552345896023 34-35 29.032112346508924 23.47351125716062 25.888100623075378 21.606275773255074 36-37 22.38908827225107 21.683406446238088 36.269488373019 19.65801690849184 38-39 20.855569933309344 28.413818978124546 31.17439875041155 19.55621233815456 40-41 20.248866266538958 30.76802929063467 27.610496423274782 21.37260801955159 42-43 18.28602841587289 30.83276101924736 20.967827143536372 29.91338342134337 44-45 23.29758955340436 21.358988414789135 25.34666821830298 29.996753813503524 46-47 29.335296292808632 29.19205537321877 26.892162019184024 14.58048631478857 48-49 24.577506519039577 25.50464180116891 28.64603031449102 21.27182136530049 50-51 11.703851035910438 29.035196915618965 38.41274726418894 20.84820478428166 52-53 20.878183672281956 33.66937274344738 22.208841552110098 23.243602032160567 54-55 16.27098842746418 24.09025904228768 28.434630024896723 31.204122505351418 56-57 25.15752016181512 21.421134267659482 26.1073112322785 27.31403433824689 58-59 32.902231531755 21.774124398631745 24.36218982259828 20.961454247014977 60-61 22.099318081072326 27.16145791464644 34.05743675961803 16.6817872446632 62-63 16.967607142337634 27.7987847032154 31.905024028026702 23.328584126420264 64-65 15.831025079200295 36.922725929464654 25.498590548637175 21.74765844269788 66-67 21.975700715626097 25.85073909238674 21.04585381688945 31.127706375097713 68-69 17.49409544516707 26.540753869062428 26.685755189692195 29.279395496078305 70-71 27.65427240628512 26.309374703337017 22.116053027263902 23.920299863113957 72-73 23.112764087472325 19.892573549451996 34.94016211962064 22.054500243455042 74-75 16.428301833345966 36.064786849702685 24.727777178069672 22.77913413888168 76-77 19.92644715716194 24.887395668639922 24.43950619553627 30.74665097866186 78-79 21.116617244350678 26.665128288475042 27.894425346748402 24.32382912042588 80-81 26.471687077204813 23.767405610830906 24.87995602470701 24.880951287257272 82-83 20.900344437137353 22.379609688950065 33.082884636143774 23.637161237768805 84-85 19.760740348309135 27.563780627103434 28.97252785952491 23.702951165062515 86-87 19.38585964476691 29.484131910439203 26.296796955582835 24.83321148921105 88-89 17.652372838910594 29.47454780937398 21.111114525255537 31.76196482645989 90-91 21.7926640063258 22.244353307406154 24.27103817074286 31.69194451552519 92-93 25.71643591887232 28.0292729683629 25.44166689634264 20.812624216422137 94-95 22.52421922011585 24.95678283624025 26.937346213502742 25.581651730141157 96-97 13.422891239739274 27.620782211524475 33.64691615487439 25.30941039386186 98-99 20.212586778707024 30.50338540310949 22.272461407017015 27.01156641116647 100-101 17.412171638911516 25.074338684289504 25.960660978851376 31.55282869794761 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2796.0 1 1656.0 2 606.0 3 716.0 4 541.5 5 442.0 6 739.5 7 1211.5 8 1587.5 9 1612.5 10 1222.0 11 799.0 12 665.0 13 667.5 14 654.5 15 605.5 16 584.5 17 590.5 18 617.5 19 669.5 20 758.5 21 849.5 22 944.0 23 1064.5 24 1259.5 25 1431.0 26 1598.5 27 1834.0 28 2106.0 29 2363.5 30 2623.5 31 2845.5 32 3048.0 33 3192.5 34 3113.5 35 3038.5 36 3024.5 37 3016.5 38 2988.0 39 2864.0 40 2812.5 41 2897.5 42 3117.5 43 3511.0 44 4320.0 45 6993.0 46 19443.0 47 75437.5 48 165010.5 49 161951.0 50 81679.0 51 33660.0 52 12018.5 53 3859.0 54 2292.0 55 1892.5 56 1720.5 57 1512.0 58 1309.5 59 1172.5 60 1035.0 61 880.5 62 719.5 63 527.5 64 377.5 65 288.0 66 216.0 67 193.0 68 166.0 69 120.0 70 81.0 71 56.0 72 43.0 73 41.5 74 35.5 75 27.5 76 26.5 77 23.0 78 18.0 79 15.5 80 14.0 81 13.0 82 8.5 83 5.5 84 4.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22339679496798337 2 0.0 3 0.0 4 0.0 5 0.02419238766616955 6 0.004134142196117581 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0027560947974117206 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.5311637763398448E-4 26-27 0.0012249310210718759 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007196469748797271 36-37 0.017225592483823254 38-39 0.012708659343620712 40-41 0.008651075336320124 42-43 0.007273027937614263 44-45 0.0036747930632156276 46-47 3.827909440849612E-4 48-49 0.0016842801539738296 50-51 1.5311637763398448E-4 52-53 0.0 54-55 0.0 56-57 0.0010718146434378914 58-59 6.124655105359379E-4 60-61 0.0022201874756927753 62-63 9.95256454620899E-4 64-65 0.0010718146434378914 66-67 0.005129398650738481 68-69 7.655818881699224E-5 70-71 0.0 72-73 0.0 74-75 3.827909440849612E-4 76-77 0.05665305972457427 78-79 0.1371922743600501 80-81 6.124655105359379E-4 82-83 5.359073217189457E-4 84-85 0.0 86-87 0.0956977360212403 88-89 0.3385403109487397 90-91 0.3719962394617653 92-93 6.124655105359379E-4 94-95 1.5311637763398448E-4 96-97 1.5311637763398448E-4 98-99 0.0 100-101 9.186982658039069E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 653098.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.436118316087326 #Duplication Level Percentage of deduplicated Percentage of total 1 83.52919963513715 12.058374087809181 2 8.226384675759954 2.3751412498583675 3 2.6314673002269786 1.1396451987297467 4 1.3470227615027257 0.7778311983806412 5 0.8591247533993764 0.6201213294176371 6 0.561082709318852 0.48599138261026675 7 0.4507753335737469 0.45552122346110385 8 0.29698139623682146 0.34298068590012526 9 0.24500965189537768 0.31832894910105375 >10 1.4297532933115547 4.035688365298929 >50 0.17924948558579581 1.8497989581961667 >100 0.17500689421098406 5.149303779830898 >500 0.020152309030355742 1.8839439104085451 >1k 0.025455548248870412 8.393686705517396 >5k 0.006363887062217603 6.7420203399796055 >10k+ 0.01697036549924694 53.37162263550034 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 69848 10.694872744978548 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 41726 6.388933973155636 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 29149 4.463189291653014 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21145 3.237645805070602 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 20889 3.1984480123963017 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 19220 2.942896778125182 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 18453 2.8254565164799157 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 17815 2.7277682675494335 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17670 2.705566392792506 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 17591 2.693470198959421 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 15958 2.4434311542831244 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 13202 2.021442417523863 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 12144 1.8594452899871075 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 11749 1.7989643208216837 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11441 1.7518044765104164 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10569 1.618286995213582 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9570 1.4653237339572316 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8091 1.2388646114365685 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7079 1.0839108372709763 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6798 1.0408851351558266 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6452 0.9879068684944678 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6042 0.9251291536645343 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3873 0.593019730576422 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3873 0.593019730576422 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3691 0.5651525498470368 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3374 0.5166146581370636 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3180 0.48691008087607063 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3067 0.46960793020343045 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2898 0.44373126238328703 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2845 0.4356160943686858 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2709 0.41479226701046396 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2599 0.3979494654707256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 0.3968776508272878 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2270 0.3475741772291448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1931 0.29566772521122403 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1728 0.2645851005515252 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1631 0.2497328119210287 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1600 0.24498620421437517 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1595 0.24422062232620526 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1468 0.22477484236668924 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 1398 0.2140566959323103 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1375 0.21053501924672866 RNA PCR Primer, Index 33 (95% over 22bp) AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1345 0.20594152791770914 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1307 0.20012310556761773 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 1263 0.1933859849517224 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 1207 0.18481146780421928 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 983 0.15051339921420676 No Hit GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT 785 0.12019635644267782 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 739 0.11315300307151455 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 706 0.10810016260959306 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 691 0.10580341694508327 RNA PCR Primer, Index 33 (95% over 24bp) GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 684 0.1047316023016454 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 679 0.10396602041347547 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.2967456645097672E-4 0.0 12-13 0.0 0.0 0.0 3.827909440849612E-4 0.0 14-15 0.0 0.0 0.0 4.5934913290195345E-4 0.0 16-17 0.0 0.0 0.0 5.359073217189457E-4 0.0 18-19 0.0 0.0 0.0 0.0012249310210718759 0.0 20-21 0.0 0.0 0.0 0.0039044676296666045 0.0 22-23 0.0 0.0 0.0 0.009033866280405084 0.0 24-25 0.0 0.0 0.0 0.017302150672640244 0.0 26-27 0.0 0.0 0.0 0.029168669939274045 0.0 28-29 0.0 0.0 0.0 0.08666386974083523 0.0 30-31 0.0 0.0 0.0 0.2155878597086502 0.0 32-33 0.0 0.0 0.0 0.49089110669455427 0.0 34-35 0.0 0.0 0.0 0.8327234197624247 0.0 36-37 0.0 0.0 0.0 1.21413631644868 0.0 38-39 0.0 0.0 0.0 1.7632882048329654 0.0 40-41 0.0 0.0 0.0 2.2906210094044077 0.0 42-43 0.0 0.0 0.0 2.8593717941258436 0.0 44-45 1.5311637763398448E-4 0.0 0.0 3.4892925717120553 0.0 46-47 1.5311637763398448E-4 0.0 0.0 4.246835850056193 0.0 48-49 1.5311637763398448E-4 0.0 0.0 4.7714891180190415 0.0 50-51 1.5311637763398448E-4 0.0 0.0 5.370556945512005 0.0 52-53 1.5311637763398448E-4 0.0 0.0 5.932264376862278 0.0 54-55 1.5311637763398448E-4 0.0 0.0 6.583851121883699 0.0 56-57 1.5311637763398448E-4 0.0 0.0 7.634076356075198 0.0 58-59 1.5311637763398448E-4 0.0 0.0 8.30602757932194 0.0 60-61 1.5311637763398448E-4 0.0 0.0 9.11899898636958 0.0 62-63 1.5311637763398448E-4 0.0 0.0 9.90088776875752 0.0 64-65 1.5311637763398448E-4 0.0 0.0 10.671445939200549 0.0 66-67 1.5311637763398448E-4 0.0 0.0 11.672903607115625 0.0 68-69 1.5311637763398448E-4 0.0 0.0 12.848148363645272 0.0 70-71 1.5311637763398448E-4 0.0 0.0 13.934968412091294 0.0 72-73 1.5311637763398448E-4 0.0 0.0 14.78017081663089 0.0 74-75 1.5311637763398448E-4 0.0 0.0 16.016279333270045 0.0 76-77 1.5311637763398448E-4 0.0 0.0 16.88146342509087 0.0 78-79 1.5311637763398448E-4 0.0 0.0 18.63633022915397 0.0 80-81 1.5311637763398448E-4 0.0 0.0 20.159914744800933 0.0 82-83 1.5311637763398448E-4 0.0 0.0 21.210905560880605 0.0 84-85 1.5311637763398448E-4 0.0 0.0 22.490346012390177 0.0 86-87 1.5311637763398448E-4 0.0 0.0 23.625091487035636 0.0 88-89 1.5311637763398448E-4 0.0 0.0 25.046777053367183 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCATAG 25 0.004984307 57.103718 1 GTACCCA 125 3.6379788E-12 41.87606 1 TTTGCGT 525 0.0 38.8681 3 GCTTTGC 505 0.0 38.63453 1 CTTTGCG 520 0.0 38.32923 2 CTACAAC 50 0.0016228033 37.96419 3 GTAAGCG 265 0.0 35.80979 94-95 TTGCGTT 575 0.0 35.488266 4 TGCCGTT 30 0.0039724014 31.631979 94-95 TTACTCG 35 0.008436963 27.113127 94-95 GCAGAGA 1270 0.0 26.526031 94-95 AAGCAGG 585 0.0 26.359983 94-95 ACCCATG 175 1.4976831E-7 24.405552 3 CAGCGTA 60 1.6490914E-4 23.879402 90-91 TACCCAT 180 1.9661638E-7 23.72762 2 AGTACTT 1975 0.0 22.464432 12-13 TATACTC 115 0.003741964 20.632711 4 GTTGATC 485 0.0 20.544481 94-95 GTACTTT 2125 0.0 20.208801 14-15 GAGTACT 2225 0.0 19.940338 12-13 >>END_MODULE