##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938743_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2043789 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.936418583327338 31.0 31.0 34.0 30.0 34.0 2 32.13243441470719 33.0 31.0 34.0 30.0 34.0 3 32.1923129050993 34.0 31.0 34.0 30.0 34.0 4 35.85848196658266 37.0 35.0 37.0 35.0 37.0 5 35.78667856613379 37.0 35.0 37.0 35.0 37.0 6 35.844327863590614 37.0 35.0 37.0 35.0 37.0 7 35.778829908566884 37.0 35.0 37.0 33.0 37.0 8 35.76028689850077 37.0 35.0 37.0 33.0 37.0 9 37.361034333779074 39.0 37.0 39.0 34.0 39.0 10-11 37.29118612537791 39.0 37.0 39.0 33.5 39.0 12-13 37.22464623305048 39.0 37.0 39.0 33.0 39.0 14-15 38.32836902439538 40.0 38.0 41.0 33.0 41.0 16-17 38.2901180112037 40.0 38.0 41.0 33.0 41.0 18-19 38.40610332084183 40.0 38.0 41.0 33.5 41.0 20-21 38.498550975663335 40.0 38.0 41.0 34.0 41.0 22-23 38.43862135474846 40.0 38.0 41.0 34.0 41.0 24-25 38.35017925040207 40.0 38.0 41.0 34.0 41.0 26-27 38.14327310695967 40.0 37.0 41.0 33.0 41.0 28-29 37.95113047384049 40.0 37.0 41.0 33.0 41.0 30-31 37.633106695456334 40.0 36.5 41.0 33.0 41.0 32-33 37.289670557968556 39.0 35.5 41.0 32.0 41.0 34-35 37.13928688333287 39.0 35.0 41.0 31.5 41.0 36-37 36.96809039484996 39.0 35.0 41.0 31.0 41.0 38-39 36.794955350087506 39.0 35.0 41.0 31.0 41.0 40-41 36.57069785579627 39.0 35.0 41.0 30.5 41.0 42-43 36.28622573073835 39.0 35.0 41.0 30.0 41.0 44-45 35.85954298609103 39.0 35.0 41.0 29.5 41.0 46-47 35.312667794963176 39.0 35.0 41.0 26.0 41.0 48-49 34.83929921337281 39.0 35.0 41.0 23.5 41.0 50-51 34.5318709514534 39.0 34.5 40.5 23.0 41.0 52-53 34.30817589291262 38.0 34.0 40.0 22.0 41.0 54-55 34.07215788909716 38.0 34.0 40.0 20.5 41.0 56-57 33.827970010602854 38.0 34.0 40.0 19.5 41.0 58-59 33.558078647061905 38.0 33.0 40.0 18.0 41.0 60-61 33.211804398594964 37.0 33.0 40.0 14.5 41.0 62-63 32.85412902212508 37.0 33.0 40.0 11.5 41.0 64-65 32.49127674138573 36.0 32.0 39.5 8.0 41.0 66-67 32.103318150748436 36.0 32.0 39.0 6.5 40.5 68-69 31.673973438549673 35.0 31.0 39.0 2.0 40.0 70-71 31.202826955228744 35.0 31.0 38.0 2.0 40.0 72-73 30.864336778405207 35.0 31.0 37.5 2.0 40.0 74-75 30.453115757057112 35.0 30.0 37.0 2.0 39.0 76-77 28.425737441585213 32.5 27.0 35.0 2.0 37.5 78-79 29.45161829327783 34.0 29.0 36.0 2.0 38.0 80-81 29.488893912238495 34.0 30.0 36.0 2.0 37.5 82-83 29.16294123317035 34.0 29.0 35.0 2.0 37.0 84-85 28.763395096069114 34.0 29.0 35.0 2.0 37.0 86-87 28.235694584910675 34.0 28.5 35.0 2.0 36.0 88-89 27.693774406262094 34.0 27.0 35.0 2.0 36.0 90-91 27.237856990129607 33.0 26.0 35.0 2.0 35.0 92-93 26.949955939678702 33.0 26.0 35.0 2.0 35.0 94-95 26.555930186530997 33.0 25.0 35.0 2.0 35.0 96-97 26.031947524915733 33.0 24.5 35.0 2.0 35.0 98-99 25.33177666579084 33.0 20.0 35.0 2.0 35.0 100-101 22.341839593030397 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03772168107067131 1101 2 0.05697611893691601 1101 3 0.06473781772712073 1101 4 0.12223287179632791 1101 5 0.07514058739679541 1101 6 0.11807431267872914 1101 7 0.11902959047914408 1101 8 0.12761774454288854 1101 9 0.14743435545678096 1101 10-11 0.12224278638421282 1101 12-13 0.15182837665383175 1101 14-15 0.32816657677629735 1101 16-17 0.2074005874087561 1101 18-19 0.18438556629202907 1101 20-21 0.15728916668649617 1101 22-23 0.13136002923877044 1101 24-25 0.19254798952099605 1101 26-27 0.15847333033964617 1101 28-29 0.1978975723024874 1101 30-31 0.1378587394609454 1101 32-33 0.11063286493934044 1101 34-35 0.20712119592995748 1101 36-37 0.17797901626597223 1101 38-39 0.29585716320304556 1101 40-41 0.40277538840853566 1101 42-43 0.24817468633901996 1101 44-45 0.2802623099170134 1101 46-47 0.3053416974861989 1101 48-49 0.32093413938233084 1101 50-51 0.3319697633269669 1101 52-53 0.3042075516777274 1101 54-55 0.1384508644763187 1101 56-57 0.020336172797570384 1101 58-59 0.11682277846652767 1101 60-61 0.08256117927702888 1101 62-63 0.05505245571465878 1101 64-65 0.07720069949164099 1101 66-67 -0.05458001176388905 1101 68-69 0.1617569147011828 1101 70-71 0.06815918299337298 1101 72-73 0.10448056463005528 1101 74-75 0.009974024041355278 1101 76-77 -0.06553239487757168 1101 78-79 0.002418895525714504 1101 80-81 0.12774030440040818 1101 82-83 0.15653679611969196 1101 84-85 0.16925218557558352 1101 86-87 0.11946039485895099 1101 88-89 0.26781808307925203 1101 90-91 0.28566943045214543 1101 92-93 0.27190154919840026 1101 94-95 0.3411599919420638 1101 96-97 0.0881034317856404 1101 98-99 0.16612761928123376 1101 100-101 0.763568695337355 1102 1 0.1866900861672427 1102 2 0.12499093754886559 1102 3 0.11989773157873884 1102 4 0.06973063238915955 1102 5 0.14094587507086231 1102 6 0.20355676930184075 1102 7 0.14263941525241108 1102 8 0.1898355255144537 1102 9 0.1615888763861335 1102 10-11 0.28506315002515237 1102 12-13 0.2836694445247687 1102 14-15 0.25704069876881874 1102 16-17 0.3495807977059968 1102 18-19 0.2937299611698805 1102 20-21 0.3972345036615863 1102 22-23 0.43975876269611547 1102 24-25 0.32266462214447955 1102 26-27 0.28657017231691384 1102 28-29 0.3242541236626195 1102 30-31 0.29793874487011607 1102 32-33 0.3394498289793546 1102 34-35 0.2417400197393178 1102 36-37 0.31382716847333114 1102 38-39 0.2920948543257751 1102 40-41 0.3250659940102807 1102 42-43 0.4057022393025065 1102 44-45 0.37464644280650106 1102 46-47 0.5405926657120403 1102 48-49 0.4912639205887146 1102 50-51 0.4408924839930961 1102 52-53 0.4755964977857232 1102 54-55 0.42741694237151506 1102 56-57 0.36617821348060886 1102 58-59 0.4812408916408373 1102 60-61 0.4499184324665819 1102 62-63 0.36489324159587966 1102 64-65 0.4443235290749783 1102 66-67 0.3695060506585719 1102 68-69 0.37938444297747154 1102 70-71 0.41090942658950524 1102 72-73 0.47717033243930373 1102 74-75 0.46458481795771434 1102 76-77 0.3757488814770653 1102 78-79 0.5098097254088678 1102 80-81 0.35658911021589645 1102 82-83 0.4079374411318568 1102 84-85 0.46817071832753854 1102 86-87 0.3971612981170871 1102 88-89 0.45469300806107427 1102 90-91 0.6242169527951127 1102 92-93 0.648004283607591 1102 94-95 0.6713525206822162 1102 96-97 0.656944957234689 1102 98-99 0.7090923952016226 1102 100-101 1.2594457750770225 1103 1 0.06905423705670799 1103 2 0.05438973635338584 1103 3 0.004732876868757785 1103 4 0.12057817076420463 1103 5 0.04678606080832992 1103 6 0.017058625723727516 1103 7 0.12442615382564526 1103 8 0.10318238897136922 1103 9 0.08119739210616927 1103 10-11 -0.001380841297788038 1103 12-13 0.12505733030003796 1103 14-15 0.11731517606457231 1103 16-17 -0.00622572152678913 1103 18-19 0.12319879265632494 1103 20-21 0.1471725699973021 1103 22-23 0.08379264517460427 1103 24-25 0.04963244522381416 1103 26-27 0.159283787877186 1103 28-29 0.10647074538768209 1103 30-31 0.1410067797943455 1103 32-33 0.11114884407529502 1103 34-35 0.08329147391000191 1103 36-37 0.053821705606530656 1103 38-39 0.23314988635659262 1103 40-41 0.1689089937593664 1103 42-43 0.3108039851966211 1103 44-45 0.32102776996552507 1103 46-47 0.40253538183670656 1103 48-49 0.386135495577669 1103 50-51 0.310985035120126 1103 52-53 0.30606659017804105 1103 54-55 0.3860301682261209 1103 56-57 0.2515382068743577 1103 58-59 9.389729676883007E-4 1103 60-61 0.15590265367458045 1103 62-63 0.2617975112074191 1103 64-65 0.15970816889101513 1103 66-67 0.07431909039457452 1103 68-69 0.1824282443704952 1103 70-71 0.23268895544900658 1103 72-73 0.26503562195536645 1103 74-75 0.27314230351446867 1103 76-77 0.25057618183627994 1103 78-79 0.11143334023864426 1103 80-81 0.10897990846142491 1103 82-83 0.20996171912825545 1103 84-85 0.22656749396569253 1103 86-87 0.2738624888314831 1103 88-89 0.455753820567228 1103 90-91 0.561747324111252 1103 92-93 0.4665315010709783 1103 94-95 0.5150494205053029 1103 96-97 0.6299966670802348 1103 98-99 0.9755175205775899 1103 100-101 1.2090746495041351 1104 1 0.06623196373946527 1104 2 0.04169960495556779 1104 3 0.031194745351889708 1104 4 0.03336726775367538 1104 5 0.029333675376150836 1104 6 0.08836806487264681 1104 7 -0.017337538451954515 1104 8 0.01727844831246017 1104 9 0.05295074136328992 1104 10-11 0.06371667329458575 1104 12-13 0.11318225655419667 1104 14-15 0.06420009853443531 1104 16-17 0.16895932074352515 1104 18-19 0.25399468436182104 1104 20-21 0.08286566398484752 1104 22-23 0.1855831676512807 1104 24-25 0.08215369731394873 1104 26-27 0.1422584639535529 1104 28-29 0.1902197129458898 1104 30-31 -0.027942420348942676 1104 32-33 0.1244977716645792 1104 34-35 0.11021946926680215 1104 36-37 0.24094480455830336 1104 38-39 0.2660134254554407 1104 40-41 0.18073676386580217 1104 42-43 0.2714441682032387 1104 44-45 0.3674998751688179 1104 46-47 0.34011849539413674 1104 48-49 0.297556507535397 1104 50-51 0.2742500386120952 1104 52-53 0.14338352775106245 1104 54-55 0.0752152275044935 1104 56-57 0.044277842328057204 1104 58-59 0.09080020853874515 1104 60-61 0.093272369353123 1104 62-63 0.0036809507879098646 1104 64-65 0.21399095536239088 1104 66-67 0.1424129450671643 1104 68-69 0.21363509308708117 1104 70-71 0.06714109957947656 1104 72-73 0.01864026557825227 1104 74-75 0.03027346002998499 1104 76-77 -0.01966180330506262 1104 78-79 0.2159818243430287 1104 80-81 0.17365089304681547 1104 82-83 0.2158095651118579 1104 84-85 0.18179034725744359 1104 86-87 0.3056561152773014 1104 88-89 0.4623247744318313 1104 90-91 0.5241276432460502 1104 92-93 0.4698531201097893 1104 94-95 0.397333250845751 1104 96-97 0.3319311201810713 1104 98-99 0.2880640934023404 1104 100-101 0.5938533481969621 1105 1 -0.04460377572495133 1105 2 -0.03361259598119659 1105 3 0.006614909232787625 1105 4 -0.06786753606595397 1105 5 -0.055481614997162865 1105 6 -0.05317120160268729 1105 7 -0.040638476089426945 1105 8 -0.01984537389255081 1105 9 -0.0068626712825334835 1105 10-11 -0.07293270976335009 1105 12-13 -0.03837206750733202 1105 14-15 0.0790596930577081 1105 16-17 -0.09515608055621527 1105 18-19 -0.0802007004650207 1105 20-21 -0.04190817842808059 1105 22-23 -0.020307991598919273 1105 24-25 -0.006827082420691966 1105 26-27 -0.12480062647013312 1105 28-29 0.03907695609448325 1105 30-31 -0.1483759020440445 1105 32-33 -0.11106952940998838 1105 34-35 -0.0336442194688189 1105 36-37 -0.15692406415048055 1105 38-39 -0.17907581897256364 1105 40-41 -0.14277230772952265 1105 42-43 -0.25720752637557354 1105 44-45 -0.1927598490326119 1105 46-47 -0.09333829107069391 1105 48-49 -0.08896654406046167 1105 50-51 -0.060016367744488264 1105 52-53 -0.26478384598006954 1105 54-55 -0.222615345782323 1105 56-57 -0.15368905142015876 1105 58-59 -0.15155616319100318 1105 60-61 -0.3169810702093372 1105 62-63 -0.4013085926969353 1105 64-65 -0.22662227211650077 1105 66-67 -0.1908338680332946 1105 68-69 -0.19038735686956443 1105 70-71 -0.3347005040151352 1105 72-73 -0.37114760486417353 1105 74-75 -0.2894612515638464 1105 76-77 -0.39001481096465795 1105 78-79 -0.48351548118188603 1105 80-81 -0.3406006754304549 1105 82-83 -0.2935757512124191 1105 84-85 -0.15677247843886377 1105 86-87 -0.22483856245101208 1105 88-89 0.10925925017286886 1105 90-91 0.17599275036848994 1105 92-93 0.12830113107265007 1105 94-95 0.14578572236944254 1105 96-97 0.11658285665747314 1105 98-99 0.20473578437386664 1105 100-101 0.6297954794457716 1106 1 -0.06532460916720595 1106 2 -0.05156579568811992 1106 3 -0.002199896351406494 1106 4 0.0031851469398631593 1106 5 -0.007983639646234053 1106 6 -0.0932279095423354 1106 7 -0.08660682938415931 1106 8 -0.06374986679885808 1106 9 -0.030306470552055487 1106 10-11 -0.01610942032215945 1106 12-13 0.026619738723276498 1106 14-15 0.06980112703460861 1106 16-17 -0.015438838566048219 1106 18-19 -0.038091806703832276 1106 20-21 -0.07783208969224376 1106 22-23 -0.031214828809019934 1106 24-25 -0.05835341783252801 1106 26-27 -0.02159783594409248 1106 28-29 0.019021572964383893 1106 30-31 -0.014029935391171477 1106 32-33 0.03481230519236078 1106 34-35 0.0011416753194666285 1106 36-37 -0.07207267757993918 1106 38-39 -0.1062199229736649 1106 40-41 -0.08971063987551275 1106 42-43 -0.015864408063222868 1106 44-45 -0.04401508069789628 1106 46-47 -0.02211823030710036 1106 48-49 -0.11054609265572424 1106 50-51 -0.0819665838381809 1106 52-53 -0.10489532286607783 1106 54-55 -0.03663416569171574 1106 56-57 -0.10822295829619577 1106 58-59 -0.03394784541741558 1106 60-61 -0.11205536740676081 1106 62-63 -0.1050273736995706 1106 64-65 -0.02642121577123646 1106 66-67 -0.0821535483836513 1106 68-69 -0.18540104783718192 1106 70-71 -0.11765660079120366 1106 72-73 0.0942970730434105 1106 74-75 0.20658081646893223 1106 76-77 0.05010379755894334 1106 78-79 0.17165461345063093 1106 80-81 0.13092480215449953 1106 82-83 0.24684705591688427 1106 84-85 0.15561507454400214 1106 86-87 0.3125223357191018 1106 88-89 0.5536053208816583 1106 90-91 0.5864060579534787 1106 92-93 0.7598806060673802 1106 94-95 0.6990249197784983 1106 96-97 0.6344200720803883 1106 98-99 0.5894428377664838 1106 100-101 0.9788920583154628 1107 1 -0.03722905944705701 1107 2 0.01472514242084344 1107 3 -0.1099698313896269 1107 4 -0.08755174341432337 1107 5 -0.015490178121382314 1107 6 -0.016327810715282 1107 7 0.02813823221268308 1107 8 0.07717043220757347 1107 9 -0.02496599415897549 1107 10-11 0.020999548239188925 1107 12-13 -0.00573476247623006 1107 14-15 -0.08785124221660112 1107 16-17 -0.0854992367810894 1107 18-19 0.009493908883605684 1107 20-21 -0.08802279804148583 1107 22-23 -0.16383912761262565 1107 24-25 -0.08472269464290605 1107 26-27 -0.14757936028047425 1107 28-29 -0.11843375467227446 1107 30-31 -0.07991070483144824 1107 32-33 0.09735060161679598 1107 34-35 0.14773894466046755 1107 36-37 0.12841872155458844 1107 38-39 0.1301641214149143 1107 40-41 0.14634017384741327 1107 42-43 -0.009343504396888136 1107 44-45 -0.05105798298512099 1107 46-47 -0.07312712223168205 1107 48-49 -0.2561080249869363 1107 50-51 -0.12535547569748928 1107 52-53 -0.12424843114884965 1107 54-55 -0.06278957242516015 1107 56-57 -0.025749937429054626 1107 58-59 -0.05470366026115414 1107 60-61 -0.10246108546926536 1107 62-63 -0.2625228546901113 1107 64-65 -0.0242855272564384 1107 66-67 -0.15960865512163736 1107 68-69 -0.016314411127385853 1107 70-71 -0.03173881576225668 1107 72-73 0.06350505340953205 1107 74-75 0.13239337842410492 1107 76-77 -0.016562417269533114 1107 78-79 0.11307354100264533 1107 80-81 -0.056127925103165666 1107 82-83 0.13733465710039994 1107 84-85 0.1690790995637883 1107 86-87 0.28200782004069325 1107 88-89 0.48734588593544004 1107 90-91 0.5985554848594923 1107 92-93 0.5150908652041117 1107 94-95 0.6370538057940323 1107 96-97 0.4501398741652167 1107 98-99 0.7172223015701036 1107 100-101 0.9603979446374957 1108 1 -0.15408570220661844 1108 2 -0.10635939014870388 1108 3 -0.16581605942303668 1108 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-3.477391541744865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 19.0 4 35.0 5 109.0 6 316.0 7 672.0 8 1250.0 9 2256.0 10 3741.0 11 5984.0 12 8514.0 13 12102.0 14 14031.0 15 19393.0 16 25912.0 17 36071.0 18 31295.0 19 17771.0 20 14368.0 21 14007.0 22 14842.0 23 15790.0 24 18235.0 25 21188.0 26 25234.0 27 29902.0 28 36155.0 29 43796.0 30 52709.0 31 63006.0 32 76737.0 33 94952.0 34 126542.0 35 181984.0 36 248845.0 37 377983.0 38 361406.0 39 46627.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01572734037511 20.80188806672086 15.4273322707637 19.755052322140333 2 15.045829094882105 23.126262055427443 40.123515685816876 21.70439316387357 3 19.43767189274431 28.865014930601934 31.195979624119712 20.501333552534042 4 11.727286916604406 18.522949286839296 38.8731419926421 30.8766218039142 5 11.29940517343033 40.83821764379787 33.70157095473163 14.160806228040174 6 26.993197438678845 38.88464024417394 18.436198648686336 15.685963668460884 7 25.356286779114672 36.727666114261304 21.230567343302074 16.685479763321947 8 25.842246924707002 33.93589064233147 20.364724538589847 19.85713789437168 9 27.400675901475154 17.208527886195686 21.66246124232981 33.72833496999935 10-11 23.909733343314794 28.298444702461943 26.82177563339464 20.970046320828615 12-13 26.705569899828212 26.62694143084242 27.669588201130352 18.997900468199017 14-15 23.12317587221748 26.948994145666006 28.784216539410945 21.14361344270557 16-17 19.126868870330394 30.50530202353715 29.391441606303715 20.976387499828746 18-19 19.033656874797405 28.400555341492208 33.70888607548485 18.856901708225536 20-21 20.9566398488298 26.604140642698436 33.352342144908306 19.08687736356346 22-23 19.958811795151064 27.02436503963961 33.13157571549705 19.885247449712274 24-25 19.7396355494623 27.356468793989986 33.79710429990572 19.106791356642002 26-27 19.77104267714786 26.88460196683901 34.31267544024891 19.031679915764215 28-29 18.906232492688822 27.75538472904982 34.18192876074781 19.15645401751355 30-31 19.257002557504713 27.802331845410656 33.77268886367428 19.167976733410345 32-33 18.94515529734234 27.88768801476082 33.58864344607002 19.578513241826823 34-35 19.639194616422554 27.41822425619213 33.62981666134524 19.312764466040083 36-37 19.864763188769984 27.122186593522486 33.74837438110257 19.26467583660496 38-39 19.647964188169674 27.587426027142197 33.63466051207923 19.129949272608897 40-41 19.386212986090264 27.863462287031403 33.55949835599755 19.19082637088079 42-43 18.834340398693172 28.41722500118656 32.79606327068146 19.952371329438815 44-45 19.422138041783814 28.193553248476583 32.262332428790494 20.121976280949113 46-47 19.859284178778918 28.628665386838847 31.31327019331239 20.19878024106985 48-49 19.851266446780954 28.586096705677544 31.16889757210749 20.39373927543401 50-51 19.871375682772353 29.02039674984826 31.144841070339467 19.963386497039924 52-53 19.845615178474883 29.537320144104896 30.50637810458908 20.11068657283115 54-55 19.32163740973261 28.900047901226593 30.936583962434476 20.84173072660632 56-57 20.043580819742154 29.215442494308363 29.90736812851033 20.83360855743915 58-59 20.030130429825896 28.778841764357338 30.307552521527505 20.883475284289265 60-61 19.68588580272133 28.94106946005415 30.952588318012857 20.420456419211668 62-63 20.096644027343334 28.736185584715447 30.722716483942325 20.444453903998898 64-65 19.644640420317362 29.145425481788973 30.611941839397318 20.59799225849635 66-67 19.635399375810543 29.044381450852725 30.152553821478488 21.167665351858243 68-69 19.608983376350135 29.252143704664164 30.142921799121723 20.995951119863975 70-71 19.825066752260916 29.173049945908286 29.796682668289826 21.205200633540976 72-73 19.75668721184036 28.691513654296013 29.94531235856539 21.606486775298233 74-75 19.383899218559257 29.729243087226713 29.555228059256606 21.331629634957423 76-77 19.510792719928023 28.829832407104934 30.000739546075433 21.65863532689161 78-79 19.41441515301988 28.648641505121137 30.351413070106165 21.585530271752816 80-81 19.383468344074824 29.15989818904896 29.86926726255365 21.58736620432256 82-83 19.39989891324398 28.96451150289976 29.770710185836208 21.86487939802005 84-85 19.224318165916348 28.663868921889684 29.59072585281553 22.521087059378438 86-87 19.23143735043755 28.860080230523717 29.535593505213413 22.372888913825324 88-89 19.64540880064756 28.257856038044544 29.492030938679783 22.60470422262812 90-91 19.27838645332807 28.178509284193137 29.762063342652972 22.781040919825816 92-93 19.434491525299332 28.6196128856746 29.658932502327783 22.286963086698282 94-95 19.285361474536135 28.23753534184188 29.50072867128864 22.976374512333344 96-97 18.965763572347033 27.986195241385115 29.60311930591627 23.444921880351583 98-99 19.276724666868255 28.01063810007506 29.036724001436546 23.67591323162014 100-101 19.402522439475373 27.47784675958783 29.85115048105822 23.268480319878577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7531.0 1 4694.0 2 2245.0 3 2668.0 4 2265.5 5 2142.5 6 3854.0 7 7688.5 8 11049.5 9 11366.5 10 8825.0 11 6529.0 12 6028.5 13 6019.0 14 6285.0 15 6438.0 16 6557.5 17 6912.0 18 7286.0 19 7883.0 20 8841.5 21 9849.5 22 10840.5 23 12128.5 24 13606.0 25 15822.5 26 19792.5 27 24162.0 28 27194.0 29 30243.0 30 34655.5 31 39446.5 32 45065.0 33 50303.5 34 54977.0 35 58820.0 36 62910.5 37 66919.0 38 65645.0 39 62348.5 40 60794.5 41 59659.5 42 57803.5 43 57752.5 44 58864.0 45 59436.0 46 61028.0 47 69341.0 48 98563.5 49 96351.0 50 66492.5 51 56368.5 52 49579.0 53 45275.0 54 41674.5 55 37774.5 56 34112.0 57 30902.0 58 29780.0 59 27721.5 60 24468.5 61 21970.0 62 18432.0 63 12569.0 64 7508.5 65 5801.5 66 4252.5 67 3023.5 68 2490.5 69 1974.5 70 1485.0 71 1150.5 72 964.5 73 1141.0 74 1027.5 75 816.5 76 598.5 77 294.0 78 170.5 79 118.0 80 74.5 81 50.5 82 27.5 83 13.0 84 9.5 85 3.0 86 2.0 87 3.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38379695751371595 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.9571491969082913E-4 16-17 0.002006077926830999 18-19 7.339309488406093E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.785745984541456E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008146633532130763 36-37 0.016464517618991002 38-39 0.010690927488111542 40-41 0.0072659163935220325 42-43 0.0030335812552078514 44-45 0.0015167906276039257 46-47 0.0011987538831063286 48-49 0.0 50-51 3.6696547442030466E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.9142983938165825E-4 60-61 4.892872992270728E-4 62-63 0.0 64-65 0.0 66-67 9.051815035700847E-4 68-69 6.85002218917902E-4 70-71 0.001859291737062877 72-73 0.0 74-75 0.0 76-77 0.09766174492572374 78-79 0.03608493831799662 80-81 4.892872992270728E-5 82-83 0.0 84-85 0.0 86-87 0.14896351824968232 88-89 0.3228317600300227 90-91 0.2750039265305763 92-93 0.0 94-95 2.446436496135364E-5 96-97 4.892872992270728E-5 98-99 1.4678618976812186E-4 100-101 6.605378539565484E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2043789.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.617338384634635 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0335502339829 26.65891969278392 2 14.172632870636907 10.946187187331624 3 5.978554953709536 6.926276390956046 4 3.1925609179296877 4.93152821125002 5 1.8657242730080208 3.6024652791588596 6 1.2856124371469357 2.978815831007883 7 0.8683803458274958 2.3474176362970516 8 0.623715043093066 1.9268971899769525 9 0.47354881019693446 1.6458475180514527 >10 2.233947690968877 15.2519470502685 >50 0.15117013819417308 4.056121239293103 >100 0.10549638341805444 7.697511939402378 >500 0.008631943935738443 2.253690777124841 >1k 0.005585375487830757 4.488655537873868 >5k 5.077614079846143E-4 1.6248985989767502 >10k+ 3.808210559884607E-4 2.662819920246691 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22610 1.1062785835524118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18361 0.8983804101108285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13349 0.6531496157382195 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9717 0.4754404686589467 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8368 0.40943561199321454 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8021 0.39245734271003513 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7041 0.344507187385782 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4869 0.23823398599366177 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4431 0.21680320228751598 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4299 0.21034460993771864 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4065 0.19889528713580512 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3830 0.1873970356039689 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3773 0.18460809799837458 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3340 0.16342195794184233 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3217 0.15740372416134935 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3066 0.15001548594302055 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2744 0.1342604349079088 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2632 0.12878041715656557 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2406 0.11772252419403371 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2384 0.11664609213573418 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2366 0.11576537499712544 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2334 0.1141996556395988 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2276 0.11136178930408179 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.10289711902745342 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2085 0.10201640188884468 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2053 0.10045068253131804 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.4464364961353644E-5 0.0 16-17 0.0 0.0 0.0 9.785745984541458E-5 0.0 18-19 0.0 0.0 0.0 1.712505547294755E-4 0.0 20-21 0.0 0.0 0.0 3.9142983938165825E-4 0.0 22-23 0.0 0.0 0.0 0.001639112452410694 0.0 24-25 0.0 0.0 0.0 0.003082509985130559 0.0 26-27 0.0 0.0 0.0 0.006213948700183825 0.0 28-29 2.4464364961353644E-5 0.0 0.0 0.01343093636378315 0.0 30-31 4.892872992270729E-5 0.0 0.0 0.03581583030342173 0.0 32-33 4.892872992270729E-5 0.0 0.0 0.07527685098608516 0.0 34-35 4.892872992270729E-5 0.0 0.0 0.13690258632373498 0.0 36-37 4.892872992270729E-5 0.0 0.0 0.23471111743922685 0.0 38-39 4.892872992270729E-5 0.0 0.0 0.3789774776163293 0.0 40-41 4.892872992270729E-5 0.0 0.0 0.5440630123755437 0.0 42-43 4.892872992270729E-5 0.0 0.0 0.7039376373979898 0.0 44-45 4.892872992270729E-5 0.0 0.0 0.8898912754692387 0.0 46-47 4.892872992270729E-5 0.0 0.0 1.1106087761505714 0.0 48-49 4.892872992270729E-5 0.0 0.0 1.3546897453699964 0.0 50-51 4.892872992270729E-5 0.0 0.0 1.6259995527914084 0.0 52-53 4.892872992270729E-5 0.0 0.0 1.8862759316152498 0.0 54-55 4.892872992270729E-5 0.0 0.0 2.15863770673 0.0 56-57 4.892872992270729E-5 0.0 0.0 2.4787294578843513 0.0 58-59 4.892872992270729E-5 0.0 0.0 2.8385758021009018 0.0 60-61 4.892872992270729E-5 0.0 0.0 3.249087846152416 0.0 62-63 4.892872992270729E-5 0.0 0.0 3.6465848480444896 0.0 64-65 4.892872992270729E-5 0.0 0.0 4.028865014930602 0.0 66-67 4.892872992270729E-5 0.0 0.0 4.453737641214431 0.0 68-69 4.892872992270729E-5 0.0 0.0 4.942095294572972 0.0 70-71 4.892872992270729E-5 0.0 0.0 5.485791341474096 0.0 72-73 4.892872992270729E-5 0.0 0.0 6.033646330418649 0.0 74-75 4.892872992270729E-5 0.0 0.0 6.5675321669702695 0.0 76-77 4.892872992270729E-5 0.0 0.0 7.118836631374373 0.0 78-79 4.892872992270729E-5 0.0 0.0 7.750066176107221 0.0 80-81 4.892872992270729E-5 0.0 0.0 8.434138749156592 0.0 82-83 4.892872992270729E-5 0.0 0.0 9.112633446994773 0.0 84-85 4.892872992270729E-5 0.0 0.0 9.791788682686912 0.0 86-87 4.892872992270729E-5 0.0 0.0 10.506979927967123 0.0 88-89 4.892872992270729E-5 0.0 0.0 11.240030159669125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 95 3.5173452E-5 29.968431 2 CATTCCG 520 0.0 26.46251 9 GTACATG 20225 0.0 24.239288 1 TACATGG 20130 0.0 23.713223 2 GAGTACT 17465 0.0 23.053713 12-13 AGTACTT 17730 0.0 22.802818 12-13 TCACGCA 125 2.2728814E-4 22.776009 4 ATCACGC 125 2.2728814E-4 22.776009 3 GGACCGT 125 2.2728814E-4 22.776009 6 ACATGGG 20005 0.0 22.67544 3 CATGGGG 5215 0.0 22.018993 4 GTATAGG 605 0.0 21.26187 1 CATGGGT 1540 0.0 21.260075 4 GTACTTT 18790 0.0 21.238647 14-15 GGGTACG 140 4.7565557E-4 20.418144 1 TACCTGG 1020 0.0 20.003439 2 TACTTTT 20480 0.0 19.787254 14-15 CATGGGA 13375 0.0 19.68954 4 GTACACG 195 1.0069398E-5 19.545574 1 ACTTTTT 20640 0.0 19.484188 16-17 >>END_MODULE