##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938742_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2043678 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6456110013417 33.0 31.0 34.0 30.0 34.0 2 31.665126306590373 33.0 31.0 34.0 30.0 34.0 3 31.647704775409824 33.0 31.0 34.0 28.0 34.0 4 35.232693212922975 37.0 35.0 37.0 33.0 37.0 5 35.294904578901374 37.0 35.0 37.0 33.0 37.0 6 35.39312014906458 37.0 35.0 37.0 33.0 37.0 7 35.32621381646228 37.0 35.0 37.0 33.0 37.0 8 35.297832143811306 37.0 35.0 37.0 33.0 37.0 9 36.84269243980705 39.0 37.0 39.0 33.0 39.0 10-11 36.733319290025136 39.0 37.0 39.0 32.0 39.0 12-13 36.625845656703255 39.0 37.0 39.0 32.0 39.0 14-15 37.685564457805974 40.0 37.0 41.0 32.0 41.0 16-17 37.61534033247899 40.0 37.0 41.0 32.0 41.0 18-19 37.67534660548286 40.0 37.0 41.0 32.0 41.0 20-21 37.61964458197426 40.0 37.0 41.0 32.0 41.0 22-23 37.60319336020645 40.0 37.0 41.0 32.0 41.0 24-25 37.52613303074163 40.0 37.0 41.0 32.0 41.0 26-27 37.365464862859994 40.0 37.0 41.0 32.0 41.0 28-29 37.17916447698708 40.0 36.5 41.0 31.0 41.0 30-31 36.87663638792412 39.0 36.0 41.0 31.0 41.0 32-33 36.576775059476105 39.0 35.0 41.0 30.0 41.0 34-35 36.23972049412872 39.0 35.0 41.0 30.0 41.0 36-37 35.9744526779659 39.0 35.0 41.0 29.5 41.0 38-39 35.75524495541861 38.0 35.0 40.5 29.0 41.0 40-41 35.562003896895696 38.5 35.0 40.5 27.5 41.0 42-43 35.40346864819213 39.0 35.0 41.0 26.5 41.0 44-45 35.05370806947083 39.0 35.0 41.0 24.5 41.0 46-47 34.508794193605844 39.0 34.5 41.0 21.5 41.0 48-49 34.04472597933726 38.0 34.0 41.0 16.5 41.0 50-51 32.853163022746244 37.5 32.5 39.5 12.0 40.5 52-53 32.903473541330875 37.5 33.0 39.5 9.0 40.5 54-55 33.390063160634895 38.0 33.0 40.0 8.0 41.0 56-57 33.300507956732915 38.0 33.0 40.0 7.0 41.0 58-59 33.12265533024283 38.0 33.0 40.0 3.5 41.0 60-61 32.813671967893185 37.5 33.0 40.0 2.0 41.0 62-63 32.443567430877074 37.0 32.5 40.0 2.0 41.0 64-65 32.06364823617028 36.5 32.0 40.0 2.0 41.0 66-67 31.678624519126792 36.0 31.0 39.0 2.0 41.0 68-69 31.275184495796303 35.5 31.0 39.0 2.0 41.0 70-71 30.851752086189702 35.0 31.0 38.5 2.0 40.0 72-73 30.42961170986819 35.0 30.0 37.5 2.0 40.0 74-75 29.967434693723767 35.0 29.5 37.0 2.0 39.5 76-77 29.505755799103383 35.0 29.0 36.5 2.0 39.0 78-79 29.064547105757363 34.5 29.0 36.0 2.0 39.0 80-81 28.584221438015188 34.0 28.5 36.0 2.0 37.5 82-83 28.15759331949554 34.0 27.5 35.0 2.0 37.0 84-85 27.776287164612036 34.0 27.0 35.0 2.0 37.0 86-87 27.325414522248614 34.0 26.0 35.0 2.0 36.0 88-89 26.822122418502328 34.0 25.0 35.0 2.0 36.0 90-91 26.31615083198038 33.0 24.0 35.0 2.0 35.5 92-93 25.8523585907369 33.0 21.5 35.0 2.0 35.0 94-95 25.326147025118438 33.0 18.5 35.0 2.0 35.0 96-97 24.704172819788635 33.0 5.0 35.0 2.0 35.0 98-99 23.9747516487431 32.0 2.0 35.0 2.0 35.0 100-101 21.653071814640075 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.042189618931637085 1101 2 0.15967740577737644 1101 3 0.21493739506300713 1101 4 0.12856827029000328 1101 5 0.21519005952728065 1101 6 0.22446574292675336 1101 7 0.18896244353712177 1101 8 0.219632730337473 1101 9 0.338641682961935 1101 10-11 0.2101769452433544 1101 12-13 0.23446357216618452 1101 14-15 0.3438247234280496 1101 16-17 0.4782539696852126 1101 18-19 0.5082329436393067 1101 20-21 0.5694738086996409 1101 22-23 0.52779476542932 1101 24-25 0.48500901552738185 1101 26-27 0.36874421298506377 1101 28-29 0.572491173701863 1101 30-31 0.44523317837974474 1101 32-33 0.2781352064923297 1101 34-35 0.4674463530270856 1101 36-37 0.5171527665280067 1101 38-39 0.4590122430356516 1101 40-41 0.5993805607958365 1101 42-43 0.7763741004711804 1101 44-45 0.9992558770556883 1101 46-47 0.7007688455760928 1101 48-49 0.6207228110643754 1101 50-51 0.7658308795844135 1101 52-53 0.7287954891450212 1101 54-55 0.8255928574686564 1101 56-57 0.8410392007107248 1101 58-59 0.6876770691803955 1101 60-61 0.600629728692958 1101 62-63 0.5731557193090993 1101 64-65 0.5505106673542173 1101 66-67 0.5185710505023486 1101 68-69 0.5714899909934417 1101 70-71 0.3534625646490994 1101 72-73 0.5795935123785974 1101 74-75 0.46481080294848454 1101 76-77 0.45206743265753957 1101 78-79 0.3721138649726221 1101 80-81 0.5160966346671856 1101 82-83 0.6782571238637765 1101 84-85 0.6363296901398598 1101 86-87 0.6604070190583933 1101 88-89 0.5662690256963891 1101 90-91 0.37466816417068216 1101 92-93 0.28823511663833656 1101 94-95 0.33364713871306506 1101 96-97 0.3783890154381524 1101 98-99 0.6050953753841064 1101 100-101 0.5300903238719243 1102 1 0.09985061237244608 1102 2 0.03550239628126661 1102 3 0.13591193530360357 1102 4 0.1050280999608404 1102 5 0.09986705429601983 1102 6 0.2407481353559291 1102 7 0.2796072727140597 1102 8 0.20042818360999348 1102 9 0.3741612093071467 1102 10-11 0.23411147147145783 1102 12-13 0.2069531111694829 1102 14-15 0.3206246818793801 1102 16-17 0.4115222813938928 1102 18-19 0.4604636894480336 1102 20-21 0.39292023994423886 1102 22-23 0.3822598721880013 1102 24-25 0.21580446879990234 1102 26-27 0.34493672587482394 1102 28-29 0.3731861464151862 1102 30-31 0.47077593328030076 1102 32-33 0.44969046061639517 1102 34-35 0.5271206237105304 1102 36-37 0.5456853959780616 1102 38-39 0.365905808186092 1102 40-41 0.5910606442190343 1102 42-43 0.5819497684321746 1102 44-45 0.6614267846394384 1102 46-47 0.7113599364424132 1102 48-49 0.5634671207811692 1102 50-51 0.5474241588841195 1102 52-53 0.4528851100236295 1102 54-55 0.4802078652946733 1102 56-57 0.44321022031294177 1102 58-59 0.3369370854128846 1102 60-61 0.40806032052721974 1102 62-63 0.43602271979077045 1102 64-65 0.2929746254145158 1102 66-67 0.3292287923527475 1102 68-69 0.3894447172050519 1102 70-71 0.37792688727855506 1102 72-73 0.01966363593495757 1102 74-75 -0.14208892321729394 1102 76-77 0.21006351404811952 1102 78-79 0.03054564725761466 1102 80-81 0.008774191564928202 1102 82-83 -0.03572639516561438 1102 84-85 -0.019170008937823724 1102 86-87 -0.1344622299392917 1102 88-89 0.06639402811496709 1102 90-91 -0.07059681549796792 1102 92-93 -0.050841199502205114 1102 94-95 -0.22057081458745742 1102 96-97 -0.3864615773940905 1102 98-99 -0.5612769882359636 1102 100-101 -0.10464293471454411 1103 1 0.14738748463622997 1103 2 0.24672910403230475 1103 3 0.30392862566388956 1103 4 0.3109587135379286 1103 5 0.3601058224791771 1103 6 0.19962788519893593 1103 7 0.2892664318270448 1103 8 0.25891686474017916 1103 9 0.4358329040473663 1103 10-11 0.2016575797822142 1103 12-13 0.33116231804999074 1103 14-15 0.41871031025299743 1103 16-17 0.4069402199597931 1103 18-19 0.5269895171456582 1103 20-21 0.5813167490954569 1103 22-23 0.46981605979384256 1103 24-25 0.44335968419026983 1103 26-27 0.5063502874869954 1103 28-29 0.5136722739916806 1103 30-31 0.5244176738634465 1103 32-33 0.4909404221742051 1103 34-35 0.5816013961654818 1103 36-37 0.6011443362433582 1103 38-39 0.6034666584579682 1103 40-41 0.6281027976961511 1103 42-43 0.8136587248183886 1103 44-45 0.7903760379680378 1103 46-47 0.6962576107641212 1103 48-49 0.6941519771363289 1103 50-51 0.730738858087598 1103 52-53 0.6998310637719669 1103 54-55 0.7456529465292334 1103 56-57 0.8300711250487254 1103 58-59 0.6830415767507461 1103 60-61 0.7684643157375675 1103 62-63 0.6490234727617405 1103 64-65 0.4767950254712616 1103 66-67 0.44211516119741034 1103 68-69 0.3791905721445481 1103 70-71 0.34853249815229503 1103 72-73 0.44653815168733146 1103 74-75 0.3108300545278979 1103 76-77 0.25822959821221403 1103 78-79 0.31805952364934953 1103 80-81 0.40971262750832693 1103 82-83 0.4462494244467514 1103 84-85 0.37339348516490034 1103 86-87 0.37350200046783755 1103 88-89 0.44048716437295 1103 90-91 0.34547420385011307 1103 92-93 0.13625893968334069 1103 94-95 -0.004489725864857519 1103 96-97 0.1580066157831368 1103 98-99 0.4085706913932441 1103 100-101 0.6113431645883374 1104 1 0.11601604143157473 1104 2 0.18093865293799638 1104 3 0.07683911835676938 1104 4 0.17804022563044697 1104 5 0.2287067185687519 1104 6 0.24441916904665106 1104 7 0.19601173439411212 1104 8 0.2582483162332707 1104 9 0.29592153559503487 1104 10-11 0.2603637624875219 1104 12-13 0.3704094874703472 1104 14-15 0.44309571478348175 1104 16-17 0.48739793133450604 1104 18-19 0.3989270469566648 1104 20-21 0.35900115705862135 1104 22-23 0.3562241461030595 1104 24-25 0.4013312943365719 1104 26-27 0.32757318390498824 1104 28-29 0.20086707768863477 1104 30-31 0.234966958790217 1104 32-33 0.3035012659527965 1104 34-35 0.3781593487650028 1104 36-37 0.40554551617282897 1104 38-39 0.4865351788154655 1104 40-41 0.31165968100901864 1104 42-43 0.3383004551470279 1104 44-45 0.3427798803626203 1104 46-47 0.5497997938291235 1104 48-49 0.5015814179013631 1104 50-51 0.7117890754635994 1104 52-53 0.649795974749459 1104 54-55 0.6829716369560828 1104 56-57 0.66336014912428 1104 58-59 0.6218219839788901 1104 60-61 0.5047060468481419 1104 62-63 0.5950821642832977 1104 64-65 0.7672786186653582 1104 66-67 0.5150717387764026 1104 68-69 0.5701141953646847 1104 70-71 0.5960613610724792 1104 72-73 0.4806937475556232 1104 74-75 0.45188108315428366 1104 76-77 0.5007949283031543 1104 78-79 0.3738686628247265 1104 80-81 0.43346662511562783 1104 82-83 0.32783081534041614 1104 84-85 0.2981563654751689 1104 86-87 0.32006527995239153 1104 88-89 0.4431454754864923 1104 90-91 0.4128764041957247 1104 92-93 0.2197292733320495 1104 94-95 0.05291259176996377 1104 96-97 0.18349282843630021 1104 98-99 0.31049125336495464 1104 100-101 0.5703029363435306 1105 1 0.2347051092792185 1105 2 0.2543745212521351 1105 3 0.2751778001261016 1105 4 0.20343211608024347 1105 5 0.29153115012274355 1105 6 0.2926908683248044 1105 7 0.23350477888649834 1105 8 0.23136385007324378 1105 9 0.28418197266770306 1105 10-11 0.39056610106258205 1105 12-13 0.3114251154478751 1105 14-15 0.30589594498220407 1105 16-17 0.35155901136285905 1105 18-19 0.3601455661386197 1105 20-21 0.38249576606380487 1105 22-23 0.2187249559909361 1105 24-25 0.3280566889901664 1105 26-27 0.3342704802134193 1105 28-29 0.4469236383520965 1105 30-31 0.4849016913557165 1105 32-33 0.38156183222639584 1105 34-35 0.3390903577518998 1105 36-37 0.3220017707939533 1105 38-39 0.4541053627457643 1105 40-41 0.5150113305124151 1105 42-43 0.47152534290610504 1105 44-45 0.6464640126582779 1105 46-47 0.7340838243647454 1105 48-49 0.5961694136480276 1105 50-51 0.7218566963369852 1105 52-53 0.6955579744293132 1105 54-55 0.611411850080458 1105 56-57 0.520042391046367 1105 58-59 0.6200721497676938 1105 60-61 0.6100865131148865 1105 62-63 0.500355772991206 1105 64-65 0.4530147305175376 1105 66-67 0.35571319855641903 1105 68-69 0.40760709693567776 1105 70-71 0.3987876818566818 1105 72-73 0.5244400792399624 1105 74-75 0.3231193921846298 1105 76-77 0.30478824720460196 1105 78-79 0.3173070465553174 1105 80-81 0.49188812506608315 1105 82-83 0.4033166527835341 1105 84-85 0.406607021389064 1105 86-87 0.2278545433164929 1105 88-89 0.48899441901158625 1105 90-91 0.4917575774469398 1105 92-93 0.4820105083253061 1105 94-95 0.4195545489164161 1105 96-97 0.37708199423273214 1105 98-99 0.36375876981044186 1105 100-101 0.8387962495281265 1106 1 -0.09914602229389402 1106 2 0.008628406133155408 1106 3 0.0495475076045615 1106 4 0.020185913822444945 1106 5 0.005803652503381329 1106 6 0.05315368467938697 1106 7 -0.07513999633949453 1106 8 -0.02370797787614265 1106 9 -0.0837620484240773 1106 10-11 -0.03956564354027137 1106 12-13 -0.0562792157930474 1106 14-15 0.10119360887173201 1106 16-17 0.23321415169353799 1106 18-19 0.03314029272953434 1106 20-21 0.08214556095064296 1106 22-23 0.11913341631070296 1106 24-25 0.007870899728551706 1106 26-27 -0.016399489518036603 1106 28-29 -0.18522064122827686 1106 30-31 -0.3684666772057099 1106 32-33 -0.17361841965360014 1106 34-35 -0.215851598498368 1106 36-37 -0.035718078128240904 1106 38-39 -0.006623726609404912 1106 40-41 0.07531151097110467 1106 42-43 0.029425720228822172 1106 44-45 -0.10375534480096604 1106 46-47 -0.13966433932982625 1106 48-49 -0.18726726245441938 1106 50-51 -0.14697253899313978 1106 52-53 -0.18394553412818482 1106 54-55 -0.3859260008391914 1106 56-57 -0.03629982796811504 1106 58-59 0.004840353532188146 1106 60-61 -0.09379416200416557 1106 62-63 -0.1887906569429063 1106 64-65 -0.2053108145415976 1106 66-67 -0.19717123584756635 1106 68-69 -0.11224754156834393 1106 70-71 -0.07225411271041082 1106 72-73 -0.0711209994968165 1106 74-75 -0.27281045804348736 1106 76-77 -0.36306714789675354 1106 78-79 -0.1343091810633652 1106 80-81 -0.19469073045496188 1106 82-83 -0.18396437897729 1106 84-85 0.011112958215047541 1106 86-87 0.04617614272980575 1106 88-89 0.07652803716880285 1106 90-91 0.05015569171637324 1106 92-93 0.1947938663440958 1106 94-95 0.3284639240776208 1106 96-97 0.2560721970407869 1106 98-99 0.3514543035159683 1106 100-101 0.9077060940771133 1107 1 0.12092809603985799 1107 2 0.15904671766731582 1107 3 0.2354325585652326 1107 4 0.3638120019102118 1107 5 0.19447268847000032 1107 6 0.08676159431902875 1107 7 -0.022645151121096774 1107 8 0.2105434879699004 1107 9 0.27136999572456943 1107 10-11 0.23193091821176637 1107 12-13 0.33547267314228435 1107 14-15 0.29057631298208975 1107 16-17 0.31654040475734035 1107 18-19 0.3307510759224712 1107 20-21 0.3749640953693856 1107 22-23 0.3649364282458549 1107 24-25 0.28335382591760094 1107 26-27 0.28011713299767393 1107 28-29 0.23201676423860818 1107 30-31 0.24445141846657492 1107 32-33 0.2758665626917818 1107 34-35 0.3913138645224805 1107 36-37 0.34203341246996644 1107 38-39 0.21183493900731065 1107 40-41 0.17205746514391507 1107 42-43 0.21246266081556797 1107 44-45 0.5598246697279734 1107 46-47 0.5254272839629976 1107 48-49 0.5411666394878765 1107 50-51 0.3638610792778749 1107 52-53 0.307700438579964 1107 54-55 0.2733066130696784 1107 56-57 0.45194093803646496 1107 58-59 0.5073615051178848 1107 60-61 0.4453246149857577 1107 62-63 0.28650760660659813 1107 64-65 0.3051363246265737 1107 66-67 0.1997248430646259 1107 68-69 0.26887703548629105 1107 70-71 0.41309375877636256 1107 72-73 0.2486883282690009 1107 74-75 0.4876809472000545 1107 76-77 0.3255326971431991 1107 78-79 0.3245262469936101 1107 80-81 0.4147762231065286 1107 82-83 0.2537237230614835 1107 84-85 0.4537795834362335 1107 86-87 0.3964457110405881 1107 88-89 0.5181189219261917 1107 90-91 0.7308525744406325 1107 92-93 0.7156069806150391 1107 94-95 0.6362256263816484 1107 96-97 0.7160520959828887 1107 98-99 0.974141789628856 1107 100-101 1.7366307795807536 1108 1 -0.013623190375803063 1108 2 0.016353301422395106 1108 3 0.09654278710164732 1108 4 0.12577706708204772 1108 5 0.04080256463215903 1108 6 0.04839002028488437 1108 7 0.14915885677668683 1108 8 0.14927061352497617 1108 9 0.07304487033592011 1108 10-11 0.24875455709246097 1108 12-13 0.16998613492805958 1108 14-15 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72-73 -2.3096490085813812 2316 74-75 -2.3819820624174497 2316 76-77 -2.415620335911246 2316 78-79 -2.255221294746203 2316 80-81 -2.0598519533946913 2316 82-83 -2.006360758702119 2316 84-85 -2.078333860625822 2316 86-87 -1.9674754997438306 2316 88-89 -1.9361421799021308 2316 90-91 -2.4663512081024237 2316 92-93 -2.396173733410965 2316 94-95 -2.268642650353538 2316 96-97 -2.3587302788732103 2316 98-99 -2.7074672112846514 2316 100-101 -2.5570618933283775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9778.0 3 2141.0 4 1829.0 5 2028.0 6 2600.0 7 3133.0 8 3922.0 9 4764.0 10 6056.0 11 8173.0 12 11707.0 13 15641.0 14 17269.0 15 21583.0 16 27666.0 17 36273.0 18 30055.0 19 17718.0 20 16141.0 21 16530.0 22 17363.0 23 18751.0 24 21427.0 25 23990.0 26 27464.0 27 31482.0 28 37178.0 29 43674.0 30 51378.0 31 62367.0 32 75953.0 33 95072.0 34 125958.0 35 181216.0 36 238795.0 37 338462.0 38 332067.0 39 66044.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.67641430744321 19.864138167901476 14.658996123840831 19.800451400814485 2 14.727744852207428 22.369837258588475 41.291981735947594 21.610436153256497 3 19.489672801686122 29.064509629060197 30.7716632405277 20.67415432872598 4 11.376588699574778 17.73682724711667 39.67578079817757 31.210803255130987 5 11.37762261428362 40.45294094277518 33.718860555420136 14.450575887521072 6 26.765549568347403 38.41401683271078 19.093512390677343 15.726921208264471 7 25.520086874887802 36.63700207083207 21.441646385068818 16.401264669211308 8 25.695169328571986 33.34389593062041 20.848261586155477 20.11267315465212 9 27.907514654816705 17.116022240234123 21.896222041667606 33.08024106328156 10-11 23.88905655215089 27.95302606582146 27.132887122962984 21.025030259064664 12-13 26.865822772396175 26.415288167092044 27.762674963407548 18.956214097104233 14-15 23.357470494758708 26.5897881818262 29.000596035773913 21.052145287641178 16-17 19.237686359527782 30.327642401693538 29.4386715096508 20.995999729127877 18-19 19.152383972189178 28.22736661617421 33.81020379186319 18.810045619773426 20-21 21.164456583904087 26.437571137307046 33.313060043503 19.08491223528587 22-23 20.085132077100546 26.8923628099962 33.09600240739937 19.926502705503886 24-25 19.769713335919647 27.15760712567382 33.851144002978025 19.22153553542851 26-27 19.848477967071208 26.716241114467632 34.3435047694334 19.091776149027762 28-29 19.06399109801752 27.574720254611623 34.20246610831302 19.15882253905784 30-31 19.312937728678882 27.676426249587788 33.817582952529015 19.193053069204318 32-33 18.933025649850215 27.787137545939878 33.63118722142842 19.64864958278149 34-35 19.716700067226707 27.31871991310125 33.59864721593524 19.365932803736804 36-37 19.92906198724688 27.041678538794837 33.725572072824455 19.30368740113383 38-39 19.7325515316695 27.4740776068146 33.61794633643438 19.17542452508152 40-41 19.441543565474024 27.742558079361174 33.616531131382075 19.199367223782723 42-43 18.85043081156028 28.344984212007517 32.7651555150412 20.039429461391002 44-45 19.42114438038803 28.057143867587413 32.365970669180236 20.155741082844315 46-47 19.871351363299773 28.43370653227084 31.440739471774297 20.25420263265509 48-49 19.948678531132984 28.418145671710132 31.24399536112092 20.389180436035964 50-51 19.918837879880947 28.856422730797565 31.34196046238975 19.882778926931742 52-53 19.86964259177154 29.336238352392403 30.765994082702157 20.028124973133895 54-55 19.30946263291964 28.731126350933632 31.231254686712102 20.728156329434626 56-57 20.073042083428184 29.076171370911798 30.14891557897958 20.701870966680445 58-59 20.152258255219284 28.56945049004336 30.518085117613598 20.760206137123756 60-61 19.807067752321643 28.630971795696798 31.265984733191626 20.295975718789936 62-63 20.238094945725756 28.49806020451292 31.03319129205336 20.230653557707964 64-65 19.788802306547673 28.91183611640735 31.00546149255902 20.293900084485955 66-67 19.71787247434886 28.876391271672 30.455886771620772 20.949849482358367 68-69 19.78648963405945 29.056180989960833 30.497904207800374 20.65942516817934 70-71 20.018151905999268 28.968751597013924 30.16486589014117 20.848230606845636 72-73 19.938601473643246 28.60404337990633 30.37283783438514 21.08451731206528 74-75 19.607638418889643 29.63408885055469 29.9748957963049 20.783376934250768 76-77 19.83557022087883 28.8598284275076 30.16688600789628 21.137715343717286 78-79 19.73389052746243 28.75064244879256 30.598488474229242 20.916978549515765 80-81 19.771697089618016 29.294223711900504 30.06485225096768 20.86922694751381 82-83 19.843903929468173 29.03216457360455 29.981895960350407 21.142035536576863 84-85 19.780312897664626 28.81689884161008 29.883674691944616 21.519113568780675 86-87 19.838747943702266 29.013704067011854 29.789485214550016 21.358062774735867 88-89 20.282630625293365 28.426596674641964 29.762305649689623 21.52846705037505 90-91 19.980375557004486 28.41399713150981 29.9605152669224 21.64511204456331 92-93 20.32141790223424 28.852828571877804 29.805005031367433 21.02074849452053 94-95 20.214947401938815 28.587591692808456 29.612575771870652 21.584885133382077 96-97 20.068985833555892 28.249401551947845 29.660100179086022 22.021512435410237 98-99 20.42908039004539 28.382437884395163 29.077245231368636 22.111236494190816 100-101 21.03610139072182 27.690865339388342 29.705485099619562 21.567548170270275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9066.0 1 5680.0 2 2795.5 3 3195.0 4 2580.5 5 2502.0 6 4223.5 7 7875.5 8 11544.0 9 12176.0 10 9461.5 11 6877.0 12 6353.0 13 6390.0 14 6514.5 15 6632.5 16 6869.5 17 7199.5 18 7515.5 19 8163.5 20 8992.5 21 9910.5 22 11077.0 23 12530.0 24 13925.0 25 16081.0 26 20043.5 27 24453.0 28 27549.5 29 30274.5 30 34463.5 31 39403.0 32 44905.0 33 50036.5 34 54987.0 35 58556.5 36 62237.5 37 66617.0 38 66000.5 39 63079.0 40 61169.0 41 59922.0 42 58382.0 43 58335.0 44 59141.0 45 59189.0 46 60764.0 47 68671.5 48 97601.0 49 96513.0 50 66635.5 51 54657.5 52 46339.5 53 42459.0 54 40052.5 55 36533.5 56 33337.5 57 30300.0 58 28940.0 59 26982.5 60 23884.5 61 21671.5 62 18575.5 63 13088.5 64 8074.0 65 6159.5 66 4521.5 67 3307.5 68 2736.0 69 2175.0 70 1683.5 71 1365.5 72 1079.0 73 1120.5 74 1025.0 75 835.0 76 665.0 77 420.0 78 288.0 79 220.5 80 162.5 81 120.5 82 92.0 83 83.5 84 61.5 85 38.5 86 28.0 87 17.0 88 15.0 89 9.5 90 5.0 91 3.5 92 4.0 93 3.5 94 2.5 95 1.5 96 1.5 97 2.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2729392790840827 2 0.16568167783770243 3 0.1941108139344848 4 0.16294152014162702 5 0.23609394434935446 6 0.19371936283504543 7 0.2389808962077196 8 0.246516329871927 9 0.1493385944361098 10-11 0.20582988122395013 12-13 0.2115303878595356 14-15 0.17282566040247044 16-17 0.2845849492924032 18-19 0.2328889384726948 20-21 0.1980987220100231 22-23 0.24287094150839808 24-25 0.23198370780524136 26-27 0.3430334915774403 28-29 0.31213332041544706 30-31 0.28801014641249745 32-33 0.19178657303156368 34-35 0.3564651574269528 36-37 0.39624637540747615 38-39 0.376624889048079 40-41 0.33953489737620113 42-43 0.3736890058022839 44-45 0.38161589056593065 46-47 0.38658242639006735 48-49 0.40478490251399685 50-51 0.3974451943995091 52-53 0.39734733162464925 54-55 0.39157342790791894 56-57 0.3714626276742226 58-59 0.34606723759809516 60-61 0.3123045802714518 62-63 0.3800745518618882 64-65 0.412418198953064 66-67 0.4176049260206353 68-69 0.42315863849393104 70-71 0.4222778735201925 72-73 0.4134702237828073 74-75 0.4091397959952595 76-77 0.3932615607742511 78-79 0.3681352933289882 80-81 0.37885126717614026 82-83 0.3887354074369837 84-85 0.38971403518558206 86-87 0.38455177381172573 88-89 0.39230739871936776 90-91 0.41400846904453636 92-93 0.4132744982330876 94-95 0.4218864224207532 96-97 0.42191088811446814 98-99 0.41990470122984147 100-101 0.3918670162324985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2043678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.09362564226304 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4682328794528 28.25326948740592 2 13.645652149914369 10.94207337886417 3 5.709707013701455 6.867685666030495 4 3.025714276799798 4.85247422057847 5 1.8096796485729891 3.6278309181153783 6 1.1559423689947932 2.7807552363904753 7 0.7919098096554114 2.2225374815531893 8 0.5808511092837494 1.8630741543612697 9 0.4506449846429566 1.6261192180654274 >10 2.1082372491113928 14.868365707438267 >50 0.141576049735751 3.8848666510844323 >100 0.09782586939778876 7.4022616094347615 >500 0.007751536986125007 2.033781714990269 >1k 0.00529073159370437 4.243824183070994 >5k 6.152013481051594E-4 1.83561786243937 >10k+ 3.691208088630956E-4 2.6954625101770273 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24035 1.1760658968780795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17816 0.8717615984514194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12789 0.6257835138412216 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9163 0.44835830302033886 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8130 0.39781217980523353 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7962 0.38959170671700727 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6796 0.33253770897372287 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5159 0.25243702775094706 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4625 0.22630766686337084 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4178 0.2044353366821975 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4012 0.1963127263688311 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3979 0.19469799058364382 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3821 0.18696683136971676 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3605 0.17639765168485447 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3074 0.15041508495956799 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3048 0.1491428688863901 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2930 0.1433689651696598 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2832 0.13857368920152782 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2671 0.13069573582531105 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2341 0.11454837797343809 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2323 0.11366761299969957 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2182 0.1067682873720811 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2111 0.10329415886455695 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2074 0.10148369752964997 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9572554971967207E-4 0.0 18-19 0.0 0.0 0.0 6.116423428739752E-4 0.0 20-21 0.0 0.0 0.0 0.0010275591360282784 0.0 22-23 0.0 0.0 0.0 0.0018593927223368848 0.0 24-25 0.0 0.0 0.0 0.0035719912823840155 0.0 26-27 0.0 0.0 0.0 0.005847300797875203 0.0 28-29 0.0 0.0 0.0 0.013456131543227456 0.0 30-31 0.0 0.0 0.0 0.033738191632928474 0.0 32-33 0.0 0.0 0.0 0.0724673847837086 0.0 34-35 0.0 0.0 0.0 0.13253066285393295 0.0 36-37 0.0 0.0 0.0 0.2247907938530434 0.0 38-39 0.0 0.0 0.0 0.3633155516671413 0.0 40-41 0.0 0.0 0.0 0.5203119082360332 0.0 42-43 0.0 0.0 0.0 0.6706780618081714 0.0 44-45 0.0 0.0 0.0 0.8466108653124416 0.0 46-47 0.0 0.0 0.0 1.0611016021114872 0.0 48-49 0.0 0.0 0.0 1.2997155129134825 0.0 50-51 0.0 0.0 0.0 1.561376107194969 0.0 52-53 0.0 0.0 0.0 1.8071584662554474 0.0 54-55 0.0 0.0 0.0 2.068231883887775 0.0 56-57 0.0 0.0 0.0 2.3769155414894128 0.0 58-59 0.0 0.0 0.0 2.7205851411034416 0.0 60-61 0.0 0.0 0.0 3.129920662648421 0.0 62-63 0.0 0.0 0.0 3.516674348894493 0.0 64-65 0.0 0.0 0.0 3.8925652671311237 0.0 66-67 0.0 0.0 0.0 4.306451407707085 0.0 68-69 0.0 0.0 0.0 4.787740534467758 0.0 70-71 0.0 0.0 0.0 5.328432365568353 0.0 72-73 0.0 0.0 0.0 5.864377852088245 0.0 74-75 0.0 0.0 0.0 6.387234192470634 0.0 76-77 0.0 0.0 0.0 6.92374238994597 0.0 78-79 0.0 0.0 0.0 7.562076804662965 0.0 80-81 0.0 0.0 0.0 8.243299580462285 0.0 82-83 0.0 2.446569371495901E-5 0.0 8.924473424874172 0.0 84-85 0.0 4.893138742991802E-5 0.0 9.599212791839028 0.0 86-87 0.0 4.893138742991802E-5 0.0 10.304950192740733 0.0 88-89 0.0 4.893138742991802E-5 0.0 11.02133017040845 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 80 1.0869246E-5 35.582245 2 TATCACG 125 7.463859E-6 26.568075 2 GTACATG 20215 0.0 26.450216 1 TACATGG 20130 0.0 26.09011 2 CATGGGG 5330 0.0 25.190184 4 ACATGGG 19875 0.0 24.849388 3 GAGTACT 16860 0.0 23.833431 12-13 GTCGGGA 1095 0.0 22.963274 2 TTCGCGA 105 3.445348E-8 22.622978 48-49 CATGGGT 1715 0.0 22.407476 4 CGGGAGT 1125 0.0 22.35092 4 AGTACTT 17195 0.0 22.347649 12-13 CATGGGA 13050 0.0 21.921932 4 GTACTTT 18555 0.0 21.631723 14-15 TCGCGAT 110 5.6414137E-8 21.593334 50-51 CAGCGCG 110 0.002899897 21.56288 9 ATGGGAT 4390 0.0 21.181837 5 ATGGGAG 3810 0.0 21.168789 5 GTATCAA 39885 0.0 20.472176 1 GTGTAGC 1125 0.0 20.278656 1 >>END_MODULE