##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938742_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2043678 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.993529802640143 31.0 31.0 34.0 30.0 34.0 2 32.17242001920068 33.0 31.0 34.0 30.0 34.0 3 32.22479226179467 34.0 31.0 34.0 30.0 34.0 4 35.881027735289024 37.0 35.0 37.0 35.0 37.0 5 35.80317006886604 37.0 35.0 37.0 35.0 37.0 6 35.86083424101057 37.0 35.0 37.0 35.0 37.0 7 35.78145334049689 37.0 35.0 37.0 33.0 37.0 8 35.781461658832754 37.0 35.0 37.0 33.0 37.0 9 37.37287870202645 39.0 37.0 39.0 34.0 39.0 10-11 37.30702341562614 39.0 37.0 39.0 34.0 39.0 12-13 37.23206126405432 39.0 37.0 39.0 33.0 39.0 14-15 38.33053250071684 40.0 38.0 41.0 33.0 41.0 16-17 38.294821640199686 40.0 38.0 41.0 33.0 41.0 18-19 38.52077773504436 40.0 38.0 41.0 34.0 41.0 20-21 38.54824585869203 40.0 38.0 41.0 34.0 41.0 22-23 38.46104523315317 40.0 38.0 41.0 34.0 41.0 24-25 38.36743141532081 40.0 38.0 41.0 34.0 41.0 26-27 38.14567803734248 40.0 37.0 41.0 33.0 41.0 28-29 37.947090246115096 40.0 37.0 41.0 33.0 41.0 30-31 37.625032906358044 39.5 36.5 41.0 33.0 41.0 32-33 37.33332110048647 39.0 35.5 41.0 32.0 41.0 34-35 37.206366903200994 40.0 35.0 41.0 32.0 41.0 36-37 37.00469252005453 39.5 35.0 41.0 31.0 41.0 38-39 36.81174040137438 39.0 35.0 41.0 31.0 41.0 40-41 36.56104728827144 39.0 35.0 41.0 30.5 41.0 42-43 36.253177114985824 39.0 35.0 41.0 30.0 41.0 44-45 35.82817400784272 39.0 35.0 41.0 29.0 41.0 46-47 35.25921206765449 39.0 35.0 41.0 26.0 41.0 48-49 34.754969716364315 39.0 35.0 41.0 23.5 41.0 50-51 34.44979517321222 38.5 34.0 40.0 22.5 41.0 52-53 34.20808879872465 38.0 34.0 40.0 21.5 41.0 54-55 33.951186537213786 38.0 34.0 40.0 20.0 41.0 56-57 33.69447461880003 38.0 33.5 40.0 18.5 41.0 58-59 33.415097681728724 38.0 33.0 40.0 16.0 41.0 60-61 33.04567206771321 37.0 33.0 40.0 13.0 41.0 62-63 32.70030699552474 37.0 32.5 40.0 9.0 41.0 64-65 32.30340126967164 36.0 32.0 39.0 7.5 41.0 66-67 31.904950779917385 36.0 31.0 39.0 4.0 40.0 68-69 31.589717900765187 35.0 31.0 39.0 2.0 40.0 70-71 31.169393368231198 35.0 31.0 38.0 2.0 40.0 72-73 30.687854202080757 35.0 30.5 37.5 2.0 39.5 74-75 30.198919056720285 35.0 30.0 37.0 2.0 39.0 76-77 28.27532982201697 32.5 27.0 35.0 2.0 37.5 78-79 29.186746395469342 34.0 29.0 36.0 2.0 38.0 80-81 29.21687394002382 34.0 29.0 35.5 2.0 37.5 82-83 28.882051379914056 34.0 29.0 35.0 2.0 37.0 84-85 28.467824676881584 34.0 29.0 35.0 2.0 36.5 86-87 27.882854833295657 34.0 27.5 35.0 2.0 36.0 88-89 27.24887041892118 33.5 26.0 35.0 2.0 36.0 90-91 26.690069815303584 33.0 25.0 35.0 2.0 35.0 92-93 26.390187446358965 33.0 25.0 35.0 2.0 35.0 94-95 26.03706087749636 33.0 24.0 35.0 2.0 35.0 96-97 25.413762344165765 32.5 21.5 35.0 2.0 35.0 98-99 24.609626859025738 32.0 9.5 34.0 2.0 35.0 100-101 21.131990704993644 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22173199713267877 1101 2 0.21853245033639013 1101 3 0.05538505278601491 1101 4 0.0580897370309188 1101 5 0.0890565253779485 1101 6 0.11726639376350789 1101 7 0.21359010197761563 1101 8 0.20817404414322738 1101 9 0.2624807537763374 1101 10-11 0.3393674565730791 1101 12-13 0.1849120527352781 1101 14-15 0.3558010966411089 1101 16-17 0.3011104748707254 1101 18-19 0.22720866870366052 1101 20-21 0.24239845298229312 1101 22-23 0.1329214449278382 1101 24-25 0.300496681531115 1101 26-27 0.15852661190793071 1101 28-29 0.2519461740889284 1101 30-31 0.2412784619853383 1101 32-33 0.255404001732785 1101 34-35 0.32316735218243053 1101 36-37 0.2917334164872116 1101 38-39 0.4043126294238917 1101 40-41 0.41494353544878493 1101 42-43 0.4760567395855375 1101 44-45 0.15058701901804028 1101 46-47 0.11916567285207691 1101 48-49 -0.021148009835357584 1101 50-51 0.028563405567666678 1101 52-53 0.08754095193717859 1101 54-55 0.0033239444827017905 1101 56-57 -0.015882242136946445 1101 58-59 0.07737418962361176 1101 60-61 0.03094908515287642 1101 62-63 -0.041278967967272706 1101 64-65 -0.14180420779532454 1101 66-67 -0.1582404840767282 1101 68-69 -0.0211107117189826 1101 70-71 0.046121949857599986 1101 72-73 0.07051231258320811 1101 74-75 -0.004119717706192461 1101 76-77 -0.025808940797563906 1101 78-79 -0.10362334176622312 1101 80-81 -0.05176414603236168 1101 82-83 0.19509972878869064 1101 84-85 0.2700791911534317 1101 86-87 0.23726563493729103 1101 88-89 0.2112205528636828 1101 90-91 0.22246935124531575 1101 92-93 0.18295062047635824 1101 94-95 0.315117199266826 1101 96-97 0.14323196070552768 1101 98-99 -0.03767534124646588 1101 100-101 0.30405734535025175 1102 1 0.2700048252220242 1102 2 0.18440144372831924 1102 3 0.16386423924685545 1102 4 0.13946656611416586 1102 5 -0.0194491381735844 1102 6 0.04346252179450261 1102 7 0.10302737214882995 1102 8 0.22115087363381747 1102 9 0.2269683558786184 1102 10-11 0.24045948381189675 1102 12-13 0.280626226431103 1102 14-15 0.3403565513800544 1102 16-17 0.35495698513925333 1102 18-19 0.2875364652699943 1102 20-21 0.3270701523328867 1102 22-23 0.28858942628389883 1102 24-25 0.38803528971192947 1102 26-27 0.1679677314407968 1102 28-29 0.38160652393276706 1102 30-31 0.3131683458018273 1102 32-33 0.189727069083375 1102 34-35 0.3684851841975103 1102 36-37 0.31777999987324534 1102 38-39 0.4137313452646296 1102 40-41 0.5300017697730794 1102 42-43 0.5639230017637331 1102 44-45 0.5855147314686846 1102 46-47 0.5583062977725248 1102 48-49 0.39960987651885205 1102 50-51 0.4518086109229671 1102 52-53 0.36646259095056877 1102 54-55 0.3578313127515358 1102 56-57 0.35115795517847204 1102 58-59 0.3098104587571271 1102 60-61 0.37192640548799005 1102 62-63 0.35960480676970974 1102 64-65 0.31671586540364416 1102 66-67 0.3259083945675627 1102 68-69 0.2621485875953624 1102 70-71 0.3904659341098409 1102 72-73 0.40286955778448785 1102 74-75 0.40019996872358377 1102 76-77 0.44020007567016606 1102 78-79 0.16255645193650636 1102 80-81 0.31118788664443997 1102 82-83 0.3514963393137407 1102 84-85 0.5697336651198306 1102 86-87 0.434688242910358 1102 88-89 0.3264208469630603 1102 90-91 0.5613175570659976 1102 92-93 0.61996925932986 1102 94-95 0.6735314659379732 1102 96-97 0.6898858674511779 1102 98-99 1.0128169798880613 1102 100-101 1.3643838689795302 1103 1 0.18143153158418102 1103 2 0.13947120018834624 1103 3 0.06562732224131906 1103 4 0.03847272380950528 1103 5 -0.030653921473700052 1103 6 0.036987368348150085 1103 7 0.04554116421574861 1103 8 0.12623437007778904 1103 9 0.18108821814148257 1103 10-11 0.16311726297413998 1103 12-13 0.276722235685007 1103 14-15 0.21928373144923796 1103 16-17 0.16004340815020157 1103 18-19 0.11278916688813467 1103 20-21 0.18965096774389423 1103 22-23 0.29570272013999244 1103 24-25 0.27173699019614617 1103 26-27 0.23820459487527756 1103 28-29 0.12759365497451114 1103 30-31 0.2946054786788821 1103 32-33 0.24034402908463903 1103 34-35 0.19391918415981024 1103 36-37 0.21225915527593742 1103 38-39 0.14170744066873198 1103 40-41 0.3168554324956574 1103 42-43 0.28359434333179223 1103 44-45 0.30674037771915863 1103 46-47 0.28984958070607547 1103 48-49 0.2828810244374722 1103 50-51 0.34573717491110756 1103 52-53 0.2744681901180357 1103 54-55 0.11153689217707807 1103 56-57 0.016321738763309668 1103 58-59 0.2050497775316984 1103 60-61 0.3397760831191974 1103 62-63 0.23364164381548846 1103 64-65 0.23656934856398237 1103 66-67 0.2797167510571512 1103 68-69 0.22767809284681562 1103 70-71 0.16337103298316435 1103 72-73 0.3475197437454973 1103 74-75 0.35969975394333176 1103 76-77 0.38364243271729137 1103 78-79 0.18121257238011523 1103 80-81 0.1095660229073836 1103 82-83 0.17098742419707946 1103 84-85 0.2640616806990863 1103 86-87 0.1194215830558143 1103 88-89 0.21301811265664838 1103 90-91 0.13105243985947368 1103 92-93 0.10101651396201916 1103 94-95 0.2020134110038292 1103 96-97 0.3957004104057411 1103 98-99 0.24281687076183545 1103 100-101 0.5939311340282849 1104 1 0.0680035784373132 1104 2 0.0745472231457498 1104 3 0.042740776095833155 1104 4 0.11288979642716157 1104 5 -0.04893539994331064 1104 6 -0.016873303797225958 1104 7 -0.006688699181417235 1104 8 -0.023142726466865327 1104 9 0.08449468781439862 1104 10-11 0.12312775058448722 1104 12-13 -0.0874380419776557 1104 14-15 -0.04367477162764999 1104 16-17 0.0025259516058540044 1104 18-19 0.061477091572818665 1104 20-21 0.08818944642462156 1104 22-23 0.006387376365317721 1104 24-25 -0.004711727050839443 1104 26-27 0.039716061850540996 1104 28-29 0.036525423930406475 1104 30-31 -0.1184084435505568 1104 32-33 -0.015191044299307066 1104 34-35 -0.00833066727385301 1104 36-37 0.13419365511564507 1104 38-39 0.141024475362876 1104 40-41 0.21666918700151427 1104 42-43 -0.04296912186067203 1104 44-45 0.019998554732033824 1104 46-47 0.017986627285530687 1104 48-49 0.16081569825617237 1104 50-51 0.09387569737981494 1104 52-53 0.08142725227349246 1104 54-55 0.018218907440306964 1104 56-57 -0.15974280744473646 1104 58-59 0.08615394794510678 1104 60-61 0.25769073957909683 1104 62-63 0.21616261903618295 1104 64-65 0.009733723529770089 1104 66-67 -0.0749650921819125 1104 68-69 0.037710132349822345 1104 70-71 0.16277261365077322 1104 72-73 0.12595700183650038 1104 74-75 0.01982665109633075 1104 76-77 0.00552107356990561 1104 78-79 3.6824644121580263E-4 1104 80-81 0.17983154613455454 1104 82-83 0.2869725265123684 1104 84-85 0.3322541171087856 1104 86-87 0.4022730695010921 1104 88-89 0.4422019654508702 1104 90-91 0.35283583209805514 1104 92-93 0.3569638100547259 1104 94-95 0.4338201437955469 1104 96-97 0.2990277098607379 1104 98-99 0.6149574575206636 1104 100-101 1.3813722939403945 1105 1 0.06377929857055165 1105 2 0.045512742884149304 1105 3 0.023563107658212346 1105 4 0.04162442761375473 1105 5 0.01490663190338637 1105 6 -0.02521371967941377 1105 7 0.047616664753611815 1105 8 0.02954238531671649 1105 9 0.10960121188998784 1105 10-11 0.08625236469674036 1105 12-13 0.0575216446624367 1105 14-15 0.00953200322733494 1105 16-17 0.15652279590157292 1105 18-19 0.14836948510591697 1105 20-21 0.13689657953588608 1105 22-23 0.10663053328918437 1105 24-25 0.18972996483351068 1105 26-27 0.274423473182118 1105 28-29 0.14236633527340814 1105 30-31 0.0364387830122439 1105 32-33 0.02686500375276779 1105 34-35 0.1474114853890569 1105 36-37 0.23407498272195681 1105 38-39 0.2071811191113042 1105 40-41 0.14480855060115516 1105 42-43 0.17003615289140583 1105 44-45 0.09443296715361527 1105 46-47 0.04487466648029681 1105 48-49 0.009650126684952909 1105 50-51 -0.19308425033001697 1105 52-53 -0.20411300963471746 1105 54-55 -0.095716833197379 1105 56-57 -0.07337612642240288 1105 58-59 0.04241642974644577 1105 60-61 -0.00779479296505059 1105 62-63 0.09588474174277906 1105 64-65 0.03829680140859182 1105 66-67 0.0025009211116824304 1105 68-69 0.07478536998045016 1105 70-71 -0.054904523119276405 1105 72-73 -0.09319246344693966 1105 74-75 -0.053023477723481705 1105 76-77 -0.031728528155824165 1105 78-79 -0.15453376665419682 1105 80-81 -0.10193940232989718 1105 82-83 0.04111891422189018 1105 84-85 -0.03957159883209371 1105 86-87 0.08204983581890346 1105 88-89 0.13631279431870524 1105 90-91 0.19017152014068017 1105 92-93 0.16865701881142314 1105 94-95 0.30563895186927326 1105 96-97 0.407967156926464 1105 98-99 0.5926841117881061 1105 100-101 1.2361055645153556 1106 1 0.08236217502535936 1106 2 -0.018817085222323726 1106 3 -0.024677176130850853 1106 4 -0.00681801824050865 1106 5 0.015801257134420155 1106 6 0.02352662475826861 1106 7 0.016255286816971193 1106 8 -0.03189396918538989 1106 9 0.050315675865398646 1106 10-11 -0.04359179855912032 1106 12-13 0.13446789604329723 1106 14-15 0.05240674314699589 1106 16-17 0.045126153464927654 1106 18-19 -0.15130277997828045 1106 20-21 -0.050819960434807854 1106 22-23 0.06082978793856597 1106 24-25 -0.0511232473822858 1106 26-27 -0.13461986339355292 1106 28-29 0.008081345318771582 1106 30-31 -0.06770198406034211 1106 32-33 -0.03459493632360022 1106 34-35 -0.1477234031824679 1106 36-37 -0.05242073074514764 1106 38-39 -0.03023359521976232 1106 40-41 0.14804407312503542 1106 42-43 0.07517938820014791 1106 44-45 0.12233760614561362 1106 46-47 0.19841199140678611 1106 48-49 0.20920162898146089 1106 50-51 0.210755736768796 1106 52-53 0.24854714106199793 1106 54-55 0.21272833603550367 1106 56-57 0.2019472283153263 1106 58-59 0.31813063114354634 1106 60-61 0.23297174516991248 1106 62-63 0.321663751185838 1106 64-65 0.1355219992601846 1106 66-67 0.02108251953045226 1106 68-69 -0.06135556068075232 1106 70-71 0.16585401218635099 1106 72-73 0.16909469126838772 1106 74-75 0.25733862939080865 1106 76-77 0.22842721099283736 1106 78-79 0.06323846315619974 1106 80-81 -0.1323183191037245 1106 82-83 0.21488882776623797 1106 84-85 0.2679056323748519 1106 86-87 0.17393807133445804 1106 88-89 0.21310905074199837 1106 90-91 0.17024019947400149 1106 92-93 0.36449489691839076 1106 94-95 0.4251866758265024 1106 96-97 0.39701299380537947 1106 98-99 0.6172257732070783 1106 100-101 1.0519524214064582 1107 1 0.07363836194908657 1107 2 0.06495182859524817 1107 3 0.03362924642193832 1107 4 0.03789451706010283 1107 5 -0.06365433345791871 1107 6 0.06007324919659851 1107 7 0.1540861455097584 1107 8 0.07049409635833825 1107 9 0.0465360783240385 1107 10-11 -0.13258928995630725 1107 12-13 0.02330812486840017 1107 14-15 0.1592104792267861 1107 16-17 0.15077459678419558 1107 18-19 0.16511009068534577 1107 20-21 0.18393403185863377 1107 22-23 0.15575858805737397 1107 24-25 0.1197836372876111 1107 26-27 0.05383641910804471 1107 28-29 0.2009191058352755 1107 30-31 0.08091390959055644 1107 32-33 0.21905289912862713 1107 34-35 0.24980805796642613 1107 36-37 0.18742126987422836 1107 38-39 0.050059884830581325 1107 40-41 -0.032027328227243856 1107 42-43 0.0983434973352999 1107 44-45 0.04105116146555332 1107 46-47 0.03847169592353339 1107 48-49 -0.03202994648787438 1107 50-51 -0.01724068510475263 1107 52-53 -0.16127394110251458 1107 54-55 0.045638349839151715 1107 56-57 0.06341248721717818 1107 58-59 0.15361521515181664 1107 60-61 0.08160617416056937 1107 62-63 0.15589760270279385 1107 64-65 -0.019613153299474106 1107 66-67 0.09835094003168621 1107 68-69 0.18992009666183662 1107 70-71 0.12643164705397325 1107 72-73 0.22075982099849156 1107 74-75 0.2810913794711105 1107 76-77 0.005376386170215852 1107 78-79 0.13252425391869593 1107 80-81 0.1855678232545941 1107 82-83 0.20710260355309984 1107 84-85 0.15663453403508854 1107 86-87 0.24653455008040837 1107 88-89 0.16638376174611835 1107 90-91 0.46629447463284635 1107 92-93 0.7510572361012926 1107 94-95 0.8302500471169871 1107 96-97 0.8110837343324988 1107 98-99 1.0474389526395278 1107 100-101 1.7129082462808825 1108 1 0.05981648569614606 1108 2 0.043188293051450444 1108 3 -0.004164367267556202 1108 4 -0.007038873766667564 1108 5 -0.02064772927754177 1108 6 0.04399248604686079 1108 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6 315.0 7 655.0 8 1224.0 9 2271.0 10 3896.0 11 5787.0 12 8528.0 13 11985.0 14 14468.0 15 19977.0 16 26497.0 17 36764.0 18 32255.0 19 18370.0 20 14961.0 21 15041.0 22 15441.0 23 16971.0 24 19194.0 25 22116.0 26 26379.0 27 31423.0 28 37530.0 29 44921.0 30 53767.0 31 64759.0 32 79006.0 33 98763.0 34 131243.0 35 185547.0 36 254010.0 37 377270.0 38 334962.0 39 37214.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.016545722418726 20.814475643417513 15.376839912190357 19.7921387219734 2 15.038474749936146 23.103052437810653 40.092470535965056 21.766002276288145 3 19.462116830537884 28.826018580226435 31.179031138956333 20.53283345027935 4 11.75865278189617 18.46249751673209 38.844475499565 30.93437420180674 5 11.26918773512042 40.78993608738086 33.735956403547156 14.204919773951568 6 26.957000859757713 38.90249505896215 18.45905189814636 15.681452183133777 7 25.32037825919739 36.78808501143527 21.13982731134748 16.751709418019864 8 25.865033532679803 33.90411796770333 20.34880250215543 19.88204599746144 9 27.28330979733598 17.22438662059287 21.734147943071267 33.75815563899988 10-11 23.8744312949496 28.291492103942012 26.84062753525751 20.993449065850882 12-13 26.732440237649964 26.593646357204996 27.69063913199633 18.983274273148705 14-15 23.10349282029752 26.97376984045432 28.79563218863246 21.127105150615705 16-17 19.09566869191222 30.49746760712177 29.463102371501947 20.943761329464056 18-19 19.017746435593082 28.39613187596089 33.72206874077032 18.86405294767571 20-21 20.920785955517456 26.58650237464023 33.396797342829935 19.095914327012377 22-23 19.97897907596011 26.995373048004627 33.11761931184854 19.908028564186726 24-25 19.70858244658923 27.384985458726003 33.80920214193566 19.097229952749117 26-27 19.753910112013177 26.86992618820259 34.34199751571152 19.034166184072713 28-29 18.912788609555907 27.736830361730174 34.18481287169505 19.165568157018864 30-31 19.223331659879882 27.770984470156257 33.83172398000076 19.173959889963097 32-33 18.921743053455582 27.868260068366936 33.62963734991521 19.58035952826228 34-35 19.617590251431217 27.402802292687056 33.65883043207843 19.32077702380329 36-37 19.85264177906544 27.13089481823699 33.75039151968365 19.266071883013918 38-39 19.61767118273129 27.57168053589851 33.6757819917274 19.134866289642808 40-41 19.455957249764193 27.8137849446843 33.55325856772519 19.176999237826326 42-43 18.885500882561786 28.421645788098065 32.752707020317764 19.94014630902239 44-45 19.436857700909652 28.14321781828173 32.306951528599356 20.112972952209265 46-47 19.868197447426507 28.588324004495846 31.30205420365295 20.24142434442469 48-49 19.92727649952462 28.573480241785333 31.126118169082385 20.37312508960766 50-51 19.856092754360706 29.024980702679358 31.133777222678237 19.985149320281696 52-53 19.863941384112373 29.541052944739825 30.493673660919185 20.101332010228617 54-55 19.333402815903483 28.878962341425606 30.917468407449704 20.870166435221204 56-57 20.0447677644694 29.229768385904098 29.83549595871716 20.88996789090934 58-59 20.095499809411407 28.74039289141245 30.25056895221291 20.913538346963232 60-61 19.748500025812056 28.92418521362965 30.895443547805034 20.43187121275326 62-63 20.154986793289286 28.715347675589552 30.687081693708045 20.44258383741311 64-65 19.691143401620984 29.157831304961825 30.56299940131173 20.588025892105463 66-67 19.644922435355863 29.005846923019142 30.120792041901524 21.22843859972347 68-69 19.643558349001612 29.183051589723892 30.176801811444836 20.996588249829657 70-71 19.88438483292243 29.109205884590832 29.871899980256174 21.134509302230565 72-73 19.81579289888133 28.663908893671113 29.97350854684544 21.546789660602112 74-75 19.381725033885118 29.69366384983877 29.50970557868932 21.414905537586794 76-77 19.541695869226064 28.846286132123762 29.968276412108473 21.643741586541697 78-79 19.467666429580685 28.652449184748452 30.267516778753702 21.61236760691716 80-81 19.40729145666679 29.157239099416145 29.803117164761904 21.63235227915516 82-83 19.412830991163617 28.92542693758996 29.720180059878054 21.941562011368365 84-85 19.235050727169348 28.65277700303081 29.544894058653075 22.567278211146764 86-87 19.281852916851065 28.854779887630023 29.41756044827496 22.445806747243957 88-89 19.71102688109475 28.212210537067016 29.45461964647945 22.622142935358784 90-91 19.286872000909753 28.114700365447824 29.723343787448332 22.875083846194087 92-93 19.442031839856337 28.605911438518543 29.582270088297253 22.36978663332787 94-95 19.2942701407736 28.199810977449165 29.438953373866596 23.066965507910638 96-97 19.03246428678903 27.935120847159517 29.50948939325769 23.522925472793762 98-99 19.321414625983156 27.976789885686493 28.970317241757265 23.73147824657309 100-101 19.509715100223936 27.32443959907978 29.805149956071215 23.360695344625064 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7169.0 1 4506.0 2 2259.0 3 2655.0 4 2200.0 5 2123.0 6 3899.0 7 7624.5 8 10790.0 9 11145.0 10 8780.5 11 6440.5 12 5878.0 13 5892.0 14 6082.5 15 6211.0 16 6488.0 17 6828.5 18 7251.5 19 7856.0 20 8592.5 21 9721.5 22 10923.5 23 12298.0 24 13729.5 25 15808.5 26 19743.5 27 24128.5 28 27159.0 29 30222.0 30 34721.5 31 39678.0 32 45361.0 33 50487.5 34 55242.0 35 58752.5 36 62598.0 37 66624.5 38 65515.0 39 62729.0 40 60999.5 41 59679.5 42 58036.0 43 58263.5 44 59231.0 45 59421.5 46 60970.5 47 69434.5 48 98706.0 49 96183.0 50 66161.0 51 56188.0 52 49486.0 53 45077.5 54 41653.5 55 38130.0 56 34337.5 57 30908.0 58 29747.0 59 27769.5 60 24507.5 61 21812.5 62 18346.0 63 12695.0 64 7653.0 65 5876.5 66 4236.5 67 3038.0 68 2548.5 69 1993.0 70 1498.5 71 1173.0 72 985.0 73 1103.0 74 975.0 75 788.0 76 601.0 77 315.0 78 188.5 79 146.0 80 98.0 81 68.5 82 49.0 83 28.5 84 17.0 85 13.0 86 7.0 87 2.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2310540114440729 2 0.0 3 0.0 4 0.0 5 0.028184479159632778 6 0.0038655796069635233 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002813554777220286 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.116423428739752E-4 26-27 0.0016881328663321718 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006703600077898769 36-37 0.01751743669991065 38-39 0.014973004553554915 40-41 0.008832115431100202 42-43 0.009541620548834014 44-45 0.0044772219498374986 46-47 7.339708114487703E-4 48-49 0.002299775209206147 50-51 2.4465693714959012E-5 52-53 0.0 54-55 0.0 56-57 9.786277485983603E-4 58-59 4.4038248686926215E-4 60-61 0.002911417552080122 62-63 9.786277485983603E-4 64-65 0.002128515353201434 66-67 0.006091957735024794 68-69 2.6912263086454913E-4 70-71 7.339708114487703E-5 72-73 0.0 74-75 3.914510994393442E-4 76-77 0.05830174812274733 78-79 0.143246636701085 80-81 0.0012722160731778686 82-83 0.0011988189920329915 84-85 0.0 86-87 0.10366114427028132 88-89 0.35186560700854047 90-91 0.38932258408614273 92-93 6.361080365889343E-4 94-95 3.1805401829446714E-4 96-97 7.339708114487703E-5 98-99 0.0 100-101 0.0014434759291825816 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2043678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.51003965348765 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03289184092048 26.584594021887714 2 14.142244973528948 10.892368294398723 3 5.964530650601015 6.890829356072627 4 3.122394901763418 4.809742059230276 5 1.9687058193980782 3.790746958553594 6 1.293559657905439 2.988902023205422 7 0.856316531014736 2.308374849572013 8 0.5944742280975228 1.8314580877609652 9 0.4623521413435334 1.6024679367312957 >10 2.288564972722791 15.454828064081427 >50 0.15268703002002673 4.049179534117425 >100 0.10623029394225046 7.78285970053783 >500 0.008671128766458543 2.2406892964860696 >1k 0.005483213778789961 4.4899231575602 >5k 5.100663980269731E-4 1.6288199012721394 >10k+ 3.8254979852022985E-4 2.654216758532338 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22566 1.10418568874353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18172 0.8891811723764702 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13312 0.6513746294670687 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9844 0.48168057786011304 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8144 0.39849721922925235 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8059 0.39433805129770927 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7122 0.34848934127587616 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4803 0.23501745382589626 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4484 0.2194083412357524 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4292 0.21001351484920813 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4233 0.20712656299084298 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3896 0.1906366854269606 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3815 0.18667324304513724 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3477 0.17013443409382498 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3236 0.1583419697232147 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3096 0.1514915754830262 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2835 0.1387204833638176 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2643 0.12932565697727333 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2490 0.12183915470049587 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2430 0.1189032714547008 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2424 0.11860968313012127 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2399 0.11738639844437333 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2329 0.11396120132427907 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2095 0.10251125666567826 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2044 0.10001575590675242 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9572554971967207E-4 0.0 18-19 0.0 0.0 0.0 6.116423428739752E-4 0.0 20-21 0.0 0.0 0.0 0.0010275591360282784 0.0 22-23 0.0 0.0 0.0 0.0018349270286219257 0.0 24-25 0.0 0.0 0.0 0.003523059894954097 0.0 26-27 0.0 0.0 0.0 0.005871766491590162 0.0 28-29 0.0 0.0 0.0 0.013602925705517209 0.0 30-31 0.0 0.0 0.0 0.03420303981351269 0.0 32-33 0.0 0.0 0.0 0.07356834100088175 0.0 34-35 0.0 0.0 0.0 0.13473257528827925 0.0 36-37 0.0 0.0 0.0 0.2286563734600069 0.0 38-39 0.0 0.0 0.0 0.36793956777926856 0.0 40-41 0.0 0.0 0.0 0.5261836747276234 0.0 42-43 0.0 0.0 0.0 0.6782868925535236 0.0 44-45 0.0 0.0 0.0 0.8559812260052708 0.0 46-47 0.0 0.0 0.0 1.0721356299769338 0.0 48-49 0.0 0.0 0.0 1.3128046590509856 0.0 50-51 0.0 0.0 0.0 1.5767650285416783 0.0 52-53 0.0 0.0 0.0 1.8243333832433486 0.0 54-55 0.0 0.0 0.0 2.0881224928780364 0.0 56-57 0.0 0.0 0.0 2.400182416212339 0.0 58-59 0.0 0.0 0.0 2.746738967684733 0.0 60-61 0.0 0.0 0.0 3.1592794951063716 0.0 62-63 0.0 0.0 0.0 3.549482844166253 0.0 64-65 0.0 0.0 0.0 3.927869263161809 0.0 66-67 0.0 0.0 0.0 4.346599611093333 0.0 68-69 0.0 0.0 0.0 4.831118209424381 0.0 70-71 0.0 0.0 0.0 5.3756511544382235 0.0 72-73 0.0 0.0 0.0 5.916832299413117 4.893138742991802E-5 74-75 0.0 0.0 0.0 6.444655175619642 4.893138742991802E-5 76-77 0.0 0.0 0.0 6.985762923513391 4.893138742991802E-5 78-79 0.0 0.0 0.0 7.627791657981345 4.893138742991802E-5 80-81 0.0 0.0 0.0 8.312561959369333 4.893138742991802E-5 82-83 0.0 0.0 0.0 8.995815387746994 4.893138742991802E-5 84-85 0.0 0.0 0.0 9.675741481779419 4.893138742991802E-5 86-87 0.0 0.0 0.0 10.384806217026362 4.893138742991802E-5 88-89 0.0 0.0 0.0 11.105394294013049 4.893138742991802E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATAG 70 0.008429168 27.118984 3 GTACATG 19815 0.0 24.458994 1 TACATGG 19740 0.0 24.161863 2 ACATGGG 19670 0.0 22.99323 3 GAGTACT 17505 0.0 22.902832 12-13 AGTACTT 17850 0.0 21.729221 12-13 CATTCCG 490 0.0 21.30152 9 CATGGGG 5105 0.0 21.195837 4 GTACTTT 18865 0.0 21.10084 14-15 GTAAGGT 760 0.0 20.606861 4 CATGGGA 13290 0.0 20.425962 4 GTCGGGA 1000 0.0 20.407036 2 TTTGCGT 305 2.1100277E-10 20.228096 3 CGGGAGT 1025 0.0 19.909304 4 GTATAGG 695 0.0 19.84594 1 ATGGGAT 4335 0.0 19.815315 5 GATTGGC 650 0.0 19.70763 9 CATGGGT 1505 0.0 19.550898 4 ACTTTTT 20730 0.0 19.534103 16-17 CATGGGC 2450 0.0 19.370705 4 >>END_MODULE