FastQCFastQC Report
Thu 26 May 2016
SRR938741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647891
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118610.44794139940050404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.357303227360945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.22674649371896352No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52710.19906408534188152No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49620.1873944206917883No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42520.16058062813008542No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30800.11631898745076742No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG24550.028.5630742
GTACATG156750.024.0432131
TACATGG154650.023.712962
ACATGGG160700.022.2600333
GAGTACT105100.022.16594512-13
GTACCTG33850.021.5672591
GTATCAA239750.021.1571941
GTACTTT110100.020.98768814-15
CATGGGA100900.020.003834
GGTATCA188000.019.6684271
AGTACTT107750.019.5773712-13
ACCTGGG35500.019.0854513
TAGCGCG1250.006121982418.9519864
TATCAAC273200.018.4351792
CATGGGG55500.018.35444
ATCAACG274550.018.3100133
TCAACGC275100.018.290634
CAACGCA276100.018.1729015
ACTTTTT133000.017.89126816-17
AACGCAG280850.017.8871126
Produced by FastQC (version 0.11.5)