FastQCFastQC Report
Thu 26 May 2016
SRR938741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647891
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110850.41863505710771326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99190.3746000118584942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63580.24011562409479845No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA53950.20374705756392542No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA53160.20076355106762325No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45830.17308114269054126No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30000.11329771504944879No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.1101631449330807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG23950.024.7645992
GTACATG154550.023.268291
TACATGG153150.022.6478582
ACATGGG155900.021.4874743
TACACCG3550.021.385455
GAGTACT105250.021.14552712-13
GTACCTG29200.020.5529521
GTACTTT111600.019.98487714-15
CATGGGG53550.019.4935054
GTATCAA241500.019.466441
TAGGACG3455.2750693E-1119.2546544
ATGGGCG3951.8189894E-1219.2198355
AGTACTT109750.019.19756712-13
CATGGGA102250.018.5619434
GTATAGG7250.018.3952851
GGTATCA192850.018.2026391
ACTTTTT129700.017.17682516-17
TATCAAC279100.016.7966982
ATCAACG277800.016.7728183
TCAACGC279200.016.6887134
Produced by FastQC (version 0.11.5)