##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938741_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647891 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05743740962147 33.0 31.0 34.0 30.0 34.0 2 32.275751909727404 34.0 31.0 34.0 30.0 34.0 3 32.36588703991214 34.0 31.0 34.0 30.0 34.0 4 35.97585361330961 37.0 35.0 37.0 35.0 37.0 5 35.88835718690837 37.0 35.0 37.0 35.0 37.0 6 35.92353801572648 37.0 35.0 37.0 35.0 37.0 7 35.86372286472517 37.0 35.0 37.0 35.0 37.0 8 35.83717947604339 37.0 35.0 37.0 35.0 37.0 9 37.498733520375275 39.0 37.0 39.0 34.0 39.0 10-11 37.42807237911228 39.0 37.0 39.0 34.0 39.0 12-13 37.35994023167872 39.0 37.0 39.0 34.0 39.0 14-15 38.59166937007603 40.0 38.0 41.0 34.0 41.0 16-17 38.52402799057816 40.0 38.0 41.0 34.0 41.0 18-19 38.564868417922035 40.0 38.0 41.0 34.0 41.0 20-21 38.64473726448709 40.0 38.0 41.0 34.0 41.0 22-23 38.60250365290716 40.0 38.0 41.0 34.0 41.0 24-25 38.54115048542406 40.0 38.0 41.0 34.0 41.0 26-27 38.37553849459815 40.0 38.0 41.0 34.0 41.0 28-29 38.218721050073434 40.0 38.0 41.0 33.0 41.0 30-31 38.00161241531468 40.0 37.0 41.0 33.0 41.0 32-33 37.78683827997452 40.0 37.0 41.0 33.0 41.0 34-35 37.75512700485028 40.0 37.0 41.0 33.0 41.0 36-37 37.672464803120675 40.0 37.0 41.0 33.0 41.0 38-39 37.57423700597947 40.0 37.0 41.0 32.5 41.0 40-41 37.41893189712114 40.0 37.0 41.0 32.0 41.0 42-43 37.2466457267312 40.0 36.0 41.0 31.5 41.0 44-45 36.99385189949284 40.0 36.0 41.0 31.0 41.0 46-47 36.68387293887853 40.0 36.0 41.0 30.0 41.0 48-49 36.39232204044653 39.5 35.5 41.0 30.0 41.0 50-51 36.156862197122166 39.0 35.0 41.0 29.5 41.0 52-53 35.944603837544676 39.0 35.0 41.0 29.0 41.0 54-55 35.71393554341927 39.0 35.0 40.5 28.0 41.0 56-57 35.475797719770185 38.5 35.0 40.0 28.0 41.0 58-59 35.19247223545078 38.0 34.5 40.0 27.0 41.0 60-61 34.85648408488114 38.0 34.0 40.0 26.5 41.0 62-63 34.4911255788097 37.5 34.0 40.0 26.0 41.0 64-65 34.135997289918656 37.0 34.0 40.0 26.0 41.0 66-67 33.75295055574418 36.0 33.0 39.0 25.5 41.0 68-69 33.31991309309937 36.0 33.0 39.0 24.5 40.0 70-71 32.855247629150895 35.0 32.0 38.5 23.5 40.0 72-73 32.505088766871445 35.0 32.0 37.5 22.5 40.0 74-75 32.08068855553344 35.0 32.0 37.0 21.5 39.0 76-77 30.01650426698078 33.0 29.0 35.0 18.5 37.5 78-79 31.07965188144074 34.0 31.0 36.0 20.0 38.5 80-81 31.132648587120844 35.0 31.0 36.0 20.0 37.5 82-83 30.831923028553668 34.0 31.0 35.0 18.5 37.0 84-85 30.45459386356916 34.0 31.0 35.0 17.0 37.0 86-87 29.92840811800788 34.0 30.0 35.0 8.5 36.0 88-89 29.415977470371704 34.0 29.5 35.0 4.0 36.0 90-91 29.010354467007893 34.0 29.0 35.0 2.0 35.0 92-93 28.751732982966445 34.0 29.0 35.0 2.0 35.0 94-95 28.3900151101386 34.0 29.0 35.0 2.0 35.0 96-97 27.898521880243557 34.0 29.0 35.0 2.0 35.0 98-99 27.200564713577712 33.0 27.0 35.0 2.0 35.0 100-101 24.08476765093427 30.0 13.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05179479460390013 1101 2 0.09674225676446468 1101 3 0.09169841424198921 1101 4 0.11398493651645936 1101 5 0.09198827071871563 1101 6 0.1671047106690935 1101 7 0.20294421286978803 1101 8 0.08183557795813812 1101 9 0.21295238229484426 1101 10-11 0.22631891614148714 1101 12-13 0.21924328654656478 1101 14-15 0.3011656878995126 1101 16-17 0.27272945194965104 1101 18-19 0.21159621241319826 1101 20-21 0.24772501414004466 1101 22-23 0.26213726961025685 1101 24-25 0.2754062039301388 1101 26-27 0.28864890687565037 1101 28-29 0.3309732112630428 1101 30-31 0.41884000246067643 1101 32-33 0.404131549530824 1101 34-35 0.43448972845116174 1101 36-37 0.3642847945370846 1101 38-39 0.32981582896351114 1101 40-41 0.40263988222229585 1101 42-43 0.3762779389838755 1101 44-45 0.37699398007570295 1101 46-47 0.5077920423048212 1101 48-49 0.3780337690084181 1101 50-51 0.44356013343028877 1101 52-53 0.4902128676240096 1101 54-55 0.5857566935256031 1101 56-57 0.5041006273888939 1101 58-59 0.5072324442804614 1101 60-61 0.5320054516843484 1101 62-63 0.43188231635032537 1101 64-65 0.3365587183072023 1101 66-67 0.4026621410575828 1101 68-69 0.43319961444172606 1101 70-71 0.3830040669926902 1101 72-73 0.48145637336136815 1101 74-75 0.5593415163088622 1101 76-77 0.3777712141368532 1101 78-79 0.5339044712896843 1101 80-81 0.6119305546293674 1101 82-83 0.6818642885219965 1101 84-85 0.6856692229486221 1101 86-87 0.781495789286538 1101 88-89 0.72687821661928 1101 90-91 0.8464722487635647 1101 92-93 0.6513365719316901 1101 94-95 0.7622313045729463 1101 96-97 0.6690109655603997 1101 98-99 0.8168118517099145 1101 100-101 1.2854505724300616 1102 1 0.055425152534141375 1102 2 0.11500698744652027 1102 3 0.07977153195593445 1102 4 0.09982940279272867 1102 5 0.0872787844456866 1102 6 0.0758893568774468 1102 7 0.11076808674715011 1102 8 0.12066176480592361 1102 9 0.16869729027884972 1102 10-11 0.20491001545997278 1102 12-13 0.11471017393816396 1102 14-15 0.1543922429660043 1102 16-17 0.21030245749614807 1102 18-19 0.2399478922176428 1102 20-21 0.20726969160057251 1102 22-23 0.2163698507769567 1102 24-25 0.17284801222434254 1102 26-27 0.07713975169003362 1102 28-29 0.0601479681690833 1102 30-31 0.0363949838563542 1102 32-33 0.022350252874957732 1102 34-35 0.10464119320349852 1102 36-37 0.11066952119658424 1102 38-39 0.16681562009996043 1102 40-41 0.1595242569080284 1102 42-43 0.12904151318072365 1102 44-45 0.053270175045959434 1102 46-47 -0.037019245609712925 1102 48-49 0.11125429023901034 1102 50-51 0.10444385831831227 1102 52-53 0.14150898198840167 1102 54-55 0.1914014243623754 1102 56-57 0.06829097170432874 1102 58-59 -0.019054967436005654 1102 60-61 -0.08723745662455684 1102 62-63 -0.11677979657523707 1102 64-65 -0.034851729511721885 1102 66-67 -0.1477354732565317 1102 68-69 -0.13884322579312425 1102 70-71 -0.1814287911116068 1102 72-73 -0.12639612255805588 1102 74-75 -0.20867721952516405 1102 76-77 -0.26958627516856026 1102 78-79 -0.27570247845156004 1102 80-81 -0.2629570845163052 1102 82-83 -0.14318347934550957 1102 84-85 -0.0937728019448194 1102 86-87 -0.03504272283141674 1102 88-89 0.0804760846639283 1102 90-91 0.13292590039463548 1102 92-93 0.10729073025025926 1102 94-95 0.13161848344524074 1102 96-97 0.27300490078176054 1102 98-99 0.3387723068777433 1102 100-101 0.698691747945059 1103 1 0.008684251265343335 1103 2 0.03905569740889803 1103 3 -0.020029177786362595 1103 4 0.009101901654148037 1103 5 0.07215534369327514 1103 6 0.07203131756347148 1103 7 0.07757342112380172 1103 8 0.145909578573459 1103 9 0.1149962073258024 1103 10-11 0.03814747346994096 1103 12-13 0.09732546121262686 1103 14-15 0.08395551609451957 1103 16-17 -0.009103431347575963 1103 18-19 0.12781258269114204 1103 20-21 0.12256200945019202 1103 22-23 -0.049969352245412324 1103 24-25 0.12116917519914949 1103 26-27 0.10251426148796128 1103 28-29 0.09826131297484864 1103 30-31 0.11552360860073918 1103 32-33 0.16863190013732066 1103 34-35 0.16088383826205188 1103 36-37 0.04089317254867808 1103 38-39 0.2311532092380375 1103 40-41 0.2248706765607409 1103 42-43 0.2232012693863794 1103 44-45 0.26518525661129644 1103 46-47 0.24211243573372343 1103 48-49 0.393488878102481 1103 50-51 0.263156693894679 1103 52-53 0.24275347628937283 1103 54-55 0.07526762479579929 1103 56-57 0.17363604645345276 1103 58-59 0.1397374925454713 1103 60-61 0.3186434399370768 1103 62-63 0.3467976163804636 1103 64-65 0.2482649928359919 1103 66-67 0.2686431598935073 1103 68-69 0.18453394935916378 1103 70-71 -0.024807586261395898 1103 72-73 0.15401505114540726 1103 74-75 0.2616085113535149 1103 76-77 0.21235686597601244 1103 78-79 0.0397504636778514 1103 80-81 0.23930113085777904 1103 82-83 0.16877026819775054 1103 84-85 0.1328616379792571 1103 86-87 0.2248927523627522 1103 88-89 0.27990358366289314 1103 90-91 0.24855581960872186 1103 92-93 0.33588536714157513 1103 94-95 0.29962383860676667 1103 96-97 0.2207405722095892 1103 98-99 0.28844356965050366 1103 100-101 0.7979544962555281 1104 1 -0.021931558931328254 1104 2 -0.02695787374084091 1104 3 -0.019641826522018846 1104 4 0.019239299906672613 1104 5 0.005146285818298679 1104 6 0.03897182089119866 1104 7 0.03362592017198551 1104 8 -0.022306593552670506 1104 9 0.012726437351012976 1104 10-11 0.027985526906341818 1104 12-13 -0.04042249178956325 1104 14-15 0.05172117400130816 1104 16-17 0.08287532517655194 1104 18-19 0.06112099622566092 1104 20-21 0.015940110067546698 1104 22-23 -0.06871793657593628 1104 24-25 -0.193942885581869 1104 26-27 -0.12361965963936683 1104 28-29 -0.040174595150013204 1104 30-31 -0.12668554059997916 1104 32-33 -0.1479184205871178 1104 34-35 -0.08038825822998064 1104 36-37 -0.17235830697652688 1104 38-39 -0.11615238018649876 1104 40-41 -0.06565045245675094 1104 42-43 -0.08622190855664513 1104 44-45 -0.08772804056120975 1104 46-47 -0.00764539442449319 1104 48-49 -0.02532277882117029 1104 50-51 0.07223288221365465 1104 52-53 0.029862088861001723 1104 54-55 -0.1081142830688222 1104 56-57 -0.08009740527402442 1104 58-59 -0.14975622188206472 1104 60-61 -0.08404479590983982 1104 62-63 -0.27301681646017073 1104 64-65 -0.0439579516119295 1104 66-67 -0.1846219763240171 1104 68-69 -0.03988640929316034 1104 70-71 -0.09528021504514328 1104 72-73 -0.15907154806711787 1104 74-75 -0.14870476124056609 1104 76-77 -0.04666697269308884 1104 78-79 -0.06112938995999784 1104 80-81 -0.1227870599286831 1104 82-83 -0.10667563756565457 1104 84-85 0.1000310580728474 1104 86-87 0.046513428847941185 1104 88-89 0.20425849019256148 1104 90-91 0.14252835080164417 1104 92-93 0.013523572626905178 1104 94-95 0.1727390403667748 1104 96-97 0.11556416172086159 1104 98-99 0.23718112380738887 1104 100-101 0.5931595333882349 1105 1 -0.031207383677347877 1105 2 -0.031280878819273994 1105 3 -0.054381706640072025 1105 4 -0.004111384143584473 1105 5 -0.0011622787209688568 1105 6 -0.0681282086918884 1105 7 0.008173457379768934 1105 8 0.0021345267072803153 1105 9 -0.08624455243813145 1105 10-11 -0.011558450352239902 1105 12-13 -0.017525385767051205 1105 14-15 -0.026071273036741616 1105 16-17 -0.08822704405327642 1105 18-19 -0.06963640717733455 1105 20-21 -0.05768553823647693 1105 22-23 -0.028235914172618948 1105 24-25 -0.08900465202102481 1105 26-27 -0.0594441650030646 1105 28-29 -0.02485287317940532 1105 30-31 -0.06315284717705083 1105 32-33 -0.023081597153556288 1105 34-35 0.047055208133336635 1105 36-37 -0.038457039951580896 1105 38-39 -0.01890721737384382 1105 40-41 0.003477641734875192 1105 42-43 -0.08371556345346676 1105 44-45 0.011499233286627941 1105 46-47 0.07841674415114852 1105 48-49 0.06153528574039768 1105 50-51 0.24294902777185712 1105 52-53 0.21332068075289357 1105 54-55 0.24444703140646595 1105 56-57 0.20033957335336794 1105 58-59 0.08581681254466389 1105 60-61 -0.02436318684802785 1105 62-63 0.13599434528654086 1105 64-65 0.03577984771141729 1105 66-67 0.015123309751508884 1105 68-69 -0.0035690578718856614 1105 70-71 -0.058550813445314986 1105 72-73 -0.12267840465252533 1105 74-75 -0.22595367770120944 1105 76-77 -0.20169131417585717 1105 78-79 -0.2680828394421759 1105 80-81 -0.0918769866518403 1105 82-83 -0.10314444971068681 1105 84-85 -0.0271983857885445 1105 86-87 0.007649011552633311 1105 88-89 0.14273013914459298 1105 90-91 0.17028825057132124 1105 92-93 0.139045678942896 1105 94-95 0.1300415879168959 1105 96-97 0.13896825968314985 1105 98-99 5.258706958848336E-4 1105 100-101 0.18440282143126652 1106 1 -0.07874398046767794 1106 2 -0.06106380263204869 1106 3 -0.05848695999905118 1106 4 -0.024754825682549608 1106 5 -0.0681574188504328 1106 6 -0.15660016432802593 1106 7 -0.03848750569910919 1106 8 -0.11964741597018502 1106 9 -0.0755359924758352 1106 10-11 -0.07456957530366282 1106 12-13 -0.027869787902496057 1106 14-15 0.04275514728221452 1106 16-17 -0.11224986135967896 1106 18-19 -0.1252076746889017 1106 20-21 -0.15792794071768412 1106 22-23 -0.17632654211399057 1106 24-25 -0.13951546297959538 1106 26-27 -0.10712778432847614 1106 28-29 -0.18516624543307358 1106 30-31 -0.1492103824883202 1106 32-33 -0.16668870270095937 1106 34-35 -0.13808524641009257 1106 36-37 -0.20482256900367446 1106 38-39 -0.2321237305903594 1106 40-41 -0.23583352444197203 1106 42-43 -0.10420518543563162 1106 44-45 -0.002211098334150563 1106 46-47 0.0025981093772955433 1106 48-49 -0.023645729291359885 1106 50-51 0.11320097563574194 1106 52-53 0.015167771405948827 1106 54-55 -0.06710029581250154 1106 56-57 -0.13184048270728965 1106 58-59 -0.07674016792276461 1106 60-61 0.02892317315475168 1106 62-63 -0.06258677642890831 1106 64-65 0.027402075154533634 1106 66-67 0.07099039546934449 1106 68-69 0.02392437783050383 1106 70-71 -0.061884900361484085 1106 72-73 -0.1058449154309784 1106 74-75 0.07734354276018962 1106 76-77 0.07043070122973916 1106 78-79 0.129785555441142 1106 80-81 -0.0025804488478406995 1106 82-83 0.18428298100032947 1106 84-85 0.10451829070425234 1106 86-87 0.11975273410411802 1106 88-89 0.33564261862423805 1106 90-91 0.26104888163045814 1106 92-93 0.39245741444418414 1106 94-95 0.3354982665079014 1106 96-97 0.28843764870477884 1106 98-99 0.3827674693553931 1106 100-101 0.8938902816649978 1107 1 -0.16097095494417957 1107 2 -0.1251017265943588 1107 3 -0.11194325182963638 1107 4 -0.01747657795061741 1107 5 -0.044828635030157216 1107 6 -0.13490628573433838 1107 7 -0.01716950933384709 1107 8 -0.05315786403639322 1107 9 -0.13522171114075832 1107 10-11 -0.0705892379584867 1107 12-13 -0.09613401486237905 1107 14-15 -0.15856627946454438 1107 16-17 -0.05185799501317945 1107 18-19 -0.09727269136639194 1107 20-21 -0.13227927017298668 1107 22-23 -0.2158376032696694 1107 24-25 -0.1430140871776544 1107 26-27 -0.12388045382216006 1107 28-29 -0.12680450805432741 1107 30-31 -0.26155099747543176 1107 32-33 -0.20044402161695984 1107 34-35 -0.1453514513133527 1107 36-37 -0.1360098459376431 1107 38-39 -0.19148246820996917 1107 40-41 -0.12815530882424042 1107 42-43 -0.16203922795776293 1107 44-45 -0.1846738203053846 1107 46-47 -0.2669214912387119 1107 48-49 -0.5028634563351702 1107 50-51 -0.37351322979301926 1107 52-53 -0.3164169188743884 1107 54-55 -0.1884938335576436 1107 56-57 -0.28025411372961173 1107 58-59 -0.40204835485666024 1107 60-61 -0.3987837262912777 1107 62-63 -0.34873642864661036 1107 64-65 -0.35124489479678545 1107 66-67 -0.3655993673964204 1107 68-69 -0.2649754185362099 1107 70-71 -0.4980374720528076 1107 72-73 -0.3519240623607871 1107 74-75 -0.31709814440361583 1107 76-77 -0.2559606104167713 1107 78-79 -0.3202950950175776 1107 80-81 -0.2699389091174531 1107 82-83 -0.25579482297161604 1107 84-85 -0.24829936627219595 1107 86-87 -0.24059059599346355 1107 88-89 0.014381551994549824 1107 90-91 0.17045688536738268 1107 92-93 0.09322888534797968 1107 94-95 0.11330369818857022 1107 96-97 0.2559975526540974 1107 98-99 0.28269594225172767 1107 100-101 0.8553517701586202 1108 1 -0.11903378421902033 1108 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5 75.0 6 173.0 7 383.0 8 884.0 9 1699.0 10 2732.0 11 4334.0 12 5947.0 13 8285.0 14 10270.0 15 13486.0 16 17506.0 17 24122.0 18 22807.0 19 15378.0 20 13966.0 21 14206.0 22 14989.0 23 17025.0 24 19470.0 25 23546.0 26 28583.0 27 35039.0 28 43149.0 29 52185.0 30 63930.0 31 78000.0 32 96626.0 33 121091.0 34 161962.0 35 227066.0 36 345868.0 37 563893.0 38 533646.0 39 65529.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.37033753504693 19.506571039194167 14.750085128876398 22.3730062968825 2 15.515328992016666 21.252687516215733 39.85216914140348 23.37981435036412 3 19.900819180245712 25.41864449858397 29.86531545293972 24.815220868230604 4 13.01688777974622 17.163961809606214 36.96303964173752 32.85611076891005 5 13.262630523688475 38.262526667449684 33.921184822184905 14.553657986676944 6 31.048898916156293 36.305233108160415 17.74310196303398 14.902766012649312 7 27.99805581120975 33.35280795168683 20.62822827676819 18.020907960335226 8 25.94785812557994 35.16406075627735 19.714293375369305 19.1737877427734 9 26.567558860995412 17.03971198210198 20.535097554997545 35.85763160190506 10-11 24.85808517042431 26.959889965259144 27.030417792877426 21.15160707143912 12-13 26.202985696918795 24.39282810357375 27.57371810244455 21.83046809706291 14-15 23.01679378703901 25.686057244199517 26.59584030116123 24.70130866760024 16-17 21.627533656352742 28.88969206276011 27.32682649197257 22.155947788914578 18-19 21.43412572675928 27.554878705079716 29.835264749035552 21.17573081912545 20-21 22.747843991282874 26.38700505175724 29.50692258611147 21.358228370848416 22-23 22.090108694051228 26.550658618500535 29.307739631276363 22.051493056171875 24-25 21.988839419749528 26.625737237673302 29.750903643692283 21.634519698884887 26-27 21.95613783977105 26.53067390363482 29.842938857639638 21.670249398954486 28-29 21.55341741786199 26.94327296705189 29.908670711898637 21.59463890318748 30-31 21.767002493682707 26.843589860760886 29.66394764739183 21.725459998164574 32-33 21.640071286922307 26.889475435355912 29.477723969755555 21.992729307966226 34-35 22.17130952333741 26.748164753946817 29.410430569223234 21.670095153492532 36-37 22.218096602949142 26.50579017864225 29.605097653785922 21.671015564622685 38-39 22.11223496782627 26.656952133070984 29.68872133962361 21.54209155947914 40-41 22.03231039919567 26.741541545246626 29.676357797248627 21.54979025830908 42-43 21.692014645484885 26.93705308053525 29.272161549767095 22.09877072421277 44-45 21.972943611973605 27.11381218015209 28.933408186784664 21.97983602108964 46-47 22.098264022907678 27.162436939308403 28.396156725126765 22.343142312657154 48-49 22.185438216844354 27.11966886841976 28.47954408982246 22.215348824913423 50-51 22.18259719341246 27.44704012931112 28.403258836558713 21.96710384071771 52-53 22.166924544854755 27.539105650496943 28.26591049253916 22.028059312109146 54-55 21.791927990993585 27.340796883255393 28.44388231992933 22.423392805821692 56-57 22.036877650930496 27.722817895449626 27.95978006647555 22.280524387144336 58-59 22.17141637838573 27.275745124401407 28.133110261794343 22.419728235418525 60-61 22.019594213998516 27.204339633294417 28.552383639173094 22.223682513533973 62-63 22.12141662930989 27.14316790230414 28.512465203439262 22.222950264946707 64-65 21.90496134470792 27.25933582613484 28.473453023557237 22.362249805600005 66-67 21.773425581465172 27.434151031862164 28.16258874860571 22.62983463806695 68-69 21.75893553626247 27.45500767881789 28.192034460925836 22.59402232399381 70-71 21.867569392620076 27.364764834022704 27.95721178685053 22.810453986506694 72-73 21.849804240431347 27.182992049144016 27.967748672434023 22.999455037990614 74-75 21.671133743798364 27.58629414881504 27.811039049568127 22.931533057818466 76-77 21.887795858295753 27.14418464332552 28.058067182001125 22.909952316377602 78-79 21.76740654128372 27.145675742810376 28.17308173629535 22.913835979610553 80-81 21.65864549861314 27.438966014253996 27.922955244167103 22.979433242965765 82-83 21.696908973972114 27.31891154129834 27.910533326334054 23.07364615839549 84-85 21.636974482710958 27.157821073450535 27.82153797116271 23.383666472675802 86-87 21.63826525006 27.098399215806573 27.96285785775468 23.300477676378744 88-89 21.77455002104029 26.861828858790187 27.88023196043781 23.483389159731708 90-91 21.494115630767073 26.968714225790475 28.05300240601532 23.484167737427125 92-93 21.48143560290057 27.10221077831376 27.914177736168146 23.502175882617525 94-95 21.336800244723158 26.990131765292364 27.874156403778105 23.798911586206376 96-97 21.258383327552 26.738785738604275 27.874142784281595 24.128688149562134 98-99 21.222900541375473 26.810511030501704 27.697143191641633 24.26944523648119 100-101 21.407282079558286 26.38430134409922 28.153704675833787 24.05471190050871 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1374.0 1 941.5 2 661.5 3 851.0 4 750.5 5 712.5 6 1209.0 7 2419.5 8 3665.0 9 4110.5 10 3694.0 11 3132.5 12 3164.0 13 3364.5 14 3544.5 15 3851.5 16 4073.0 17 4248.0 18 4473.5 19 4855.5 20 5447.5 21 6305.5 22 7334.0 23 8724.5 24 10342.0 25 12540.5 26 15910.5 27 20164.0 28 23996.5 29 27636.0 30 31943.0 31 37000.0 32 42287.0 33 46868.0 34 52193.5 35 57090.0 36 62539.0 37 68217.5 38 71534.5 39 73565.5 40 76057.5 41 79578.0 42 81993.0 43 85067.0 44 89081.0 45 92102.5 46 94181.5 47 99689.5 48 118657.5 49 119094.0 50 103031.0 51 97950.5 52 92609.0 53 89373.5 54 85751.5 55 78592.5 56 70878.5 57 62909.0 58 56801.5 59 51112.0 60 43311.5 61 35625.0 62 28320.5 63 21132.0 64 15139.5 65 11096.5 66 8030.5 67 6106.0 68 4758.0 69 3599.0 70 2717.5 71 1963.5 72 1479.0 73 1191.0 74 937.0 75 712.5 76 482.0 77 269.0 78 171.0 79 115.5 80 64.5 81 41.0 82 28.0 83 18.0 84 8.5 85 7.0 86 5.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40458614044158164 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.643613351153805E-4 16-17 0.002360369063530183 18-19 2.2659543009889756E-4 20-21 1.8882952508241463E-5 22-23 0.0 24-25 0.0 26-27 1.8882952508241465E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00836514796115097 36-37 0.018014336692862357 38-39 0.011764079412634432 40-41 0.008667275201282833 42-43 0.00424866431435433 44-45 0.0019071782033323879 46-47 0.0016994657257417317 48-49 3.7765905016482926E-5 50-51 5.098397177225195E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.154249551813122E-4 60-61 6.609033377884513E-4 62-63 0.0 64-65 0.0 66-67 0.0012273919130356952 68-69 8.497328628708659E-4 70-71 0.002228188395972493 72-73 0.0 74-75 0.0 76-77 0.10378070698529508 78-79 0.03525447233288682 80-81 5.664885752472439E-5 82-83 0.0 84-85 0.0 86-87 0.1569362182959948 88-89 0.3363620330293052 90-91 0.28828603594332247 92-93 0.0 94-95 3.7765905016482926E-5 96-97 5.664885752472439E-5 98-99 2.2659543009889756E-4 100-101 7.930840053461416E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.857002150297674 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18715195875441 41.99728908634903 2 14.204930143625335 15.868896271546173 3 4.829630799541137 8.093060938653396 4 2.051328184427472 4.583241712341261 5 1.0736579564320141 2.998565739055361 6 0.6104282593653256 2.0458015557582874 7 0.39464652988022186 1.5430640447688948 8 0.278018025776644 1.2423402770902015 9 0.20670050906687032 1.039110370147422 >10 1.0467875726114337 10.60261524842947 >50 0.07748729622397793 2.9703782906895086 >100 0.03605638817286083 3.7053036187605137 >500 0.0018923091793342209 0.8096521791051062 >1k 9.461545896599583E-4 1.0634423506832238 >5k 2.7032988275998813E-4 1.0187846423711917 >10k+ 6.758247068999703E-5 0.4184536742509508 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11085 0.41863505710771326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9919 0.3746000118584942 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6358 0.24011562409479845 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5395 0.20374705756392542 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5316 0.20076355106762325 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4583 0.17308114269054126 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3000 0.11329771504944879 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2917 0.1101631449330807 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.7765905016482926E-5 0.0 2 0.0 0.0 0.0 3.7765905016482926E-5 0.0 3 0.0 0.0 0.0 3.7765905016482926E-5 0.0 4 0.0 0.0 0.0 3.7765905016482926E-5 0.0 5 7.553181003296585E-5 0.0 0.0 3.7765905016482926E-5 0.0 6 7.553181003296585E-5 0.0 0.0 7.553181003296585E-5 0.0 7 7.553181003296585E-5 0.0 0.0 7.553181003296585E-5 0.0 8 7.553181003296585E-5 0.0 0.0 1.510636200659317E-4 0.0 9 7.553181003296585E-5 0.0 0.0 1.510636200659317E-4 0.0 10-11 7.553181003296585E-5 0.0 0.0 2.2659543009889759E-4 0.0 12-13 1.510636200659317E-4 0.0 0.0 2.643613351153805E-4 0.0 14-15 1.510636200659317E-4 0.0 0.0 3.2101019264010487E-4 0.0 16-17 1.8882952508241465E-4 0.0 0.0 3.398931451483464E-4 0.0 18-19 1.8882952508241465E-4 0.0 0.0 4.1542495518131226E-4 0.0 20-21 1.8882952508241465E-4 0.0 0.0 5.476056227390025E-4 0.0 22-23 1.8882952508241465E-4 0.0 0.0 8.874987678873488E-4 0.0 24-25 2.2659543009889759E-4 0.0 0.0 0.0013595725805933855 0.0 26-27 3.021272401318634E-4 0.0 0.0 0.0023226031585137003 0.0 28-29 3.021272401318634E-4 0.0 0.0 0.006155842517686718 0.0 30-31 4.154249551813122E-4 0.0 0.0 0.016484817539694798 0.0 32-33 4.154249551813122E-4 0.0 0.0 0.03865340378437028 0.0 34-35 4.154249551813122E-4 0.0 0.0 0.0726427182992049 0.0 36-37 4.154249551813122E-4 0.0 0.0 0.1284607259135667 0.0 38-39 4.154249551813122E-4 0.0 0.0 0.2077502434956726 0.0 40-41 4.154249551813122E-4 0.0 0.0 0.29287459340282507 0.0 42-43 4.154249551813122E-4 0.0 0.0 0.37728139111466447 0.0 44-45 4.5319086019779517E-4 0.0 0.0 0.4852163476517727 0.0 46-47 4.5319086019779517E-4 0.0 0.0 0.6173403663519381 0.0 48-49 4.7207381270603663E-4 0.0 0.0 0.7597744771216036 0.0 50-51 4.909567652142781E-4 0.0 0.0 0.9109513949025847 0.0 52-53 4.909567652142781E-4 0.0 0.0 1.055009439587959 0.0 54-55 5.098397177225195E-4 0.0 0.0 1.220650698990253 0.0 56-57 5.28722670230761E-4 0.0 0.0 1.41858180718164 0.0 58-59 5.28722670230761E-4 1.8882952508241463E-5 0.0 1.6386248527601777 0.0 60-61 5.28722670230761E-4 3.7765905016482926E-5 0.0 1.889824769977314 0.0 62-63 5.28722670230761E-4 3.7765905016482926E-5 0.0 2.132262997230626 0.0 64-65 5.28722670230761E-4 3.7765905016482926E-5 0.0 2.3736815450484934 0.0 66-67 5.476056227390025E-4 3.7765905016482926E-5 0.0 2.6560949827617524 0.0 68-69 5.664885752472439E-4 3.7765905016482926E-5 0.0 2.9819392112439673 0.0 70-71 5.664885752472439E-4 3.7765905016482926E-5 0.0 3.338808130697223 0.0 72-73 5.664885752472439E-4 3.7765905016482926E-5 0.0 3.692448065271569 0.0 74-75 5.664885752472439E-4 3.7765905016482926E-5 0.0 4.052621501413767 0.0 76-77 5.664885752472439E-4 3.7765905016482926E-5 0.0 4.438230274584566 0.0 78-79 5.664885752472439E-4 3.7765905016482926E-5 0.0 4.879468225844644 0.0 80-81 5.664885752472439E-4 3.7765905016482926E-5 0.0 5.35713139249312 0.0 82-83 5.664885752472439E-4 3.7765905016482926E-5 0.0 5.851241610776275 0.0 84-85 5.664885752472439E-4 3.7765905016482926E-5 0.0 6.344558745054083 0.0 86-87 5.664885752472439E-4 3.7765905016482926E-5 0.0 6.866729030764484 0.0 88-89 5.664885752472439E-4 3.7765905016482926E-5 0.0 7.4134093888305825 3.7765905016482926E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2395 0.0 24.764599 2 GTACATG 15455 0.0 23.26829 1 TACATGG 15315 0.0 22.647858 2 ACATGGG 15590 0.0 21.487474 3 TACACCG 355 0.0 21.38545 5 GAGTACT 10525 0.0 21.145527 12-13 GTACCTG 2920 0.0 20.552952 1 GTACTTT 11160 0.0 19.984877 14-15 CATGGGG 5355 0.0 19.493505 4 GTATCAA 24150 0.0 19.46644 1 TAGGACG 345 5.2750693E-11 19.254654 4 ATGGGCG 395 1.8189894E-12 19.219835 5 AGTACTT 10975 0.0 19.197567 12-13 CATGGGA 10225 0.0 18.561943 4 GTATAGG 725 0.0 18.395285 1 GGTATCA 19285 0.0 18.202639 1 ACTTTTT 12970 0.0 17.176825 16-17 TATCAAC 27910 0.0 16.796698 2 ATCAACG 27780 0.0 16.772818 3 TCAACGC 27920 0.0 16.688713 4 >>END_MODULE