FastQCFastQC Report
Thu 26 May 2016
SRR938740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654485
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116750.4398216603220587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.3532888677088023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58940.22203930329235239No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52880.1992100162555072No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA49390.18606245655937026No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42410.15976733716709643No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT32210.12134180453082237No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.10269411957498348No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG26650.028.3049562
GTACATG156450.023.3606681
TACATGG155050.023.1931672
GTACCTG33800.022.2164611
GTACCGA1503.0652667E-522.1383276
ACATGGG156800.021.8753433
GAGTACT99050.021.39590312-13
CATGGGG55650.020.8863964
AGTACTT103000.019.79199412-13
GTACTTT105800.019.73962414-15
ACCTGGG36550.019.7296243
GTATCAA239500.019.6455481
GGTATCA190450.018.7659841
CATGGGA101050.018.122314
GTACACG4600.017.5640871
TATCAAC272900.017.1409932
ATCAACG271700.017.111933
ACTTTTT126700.017.08300216-17
TAGACCG2504.618216E-617.0791035
TCAACGC273350.017.0433524
Produced by FastQC (version 0.11.5)