##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938740_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2654485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56638632352415 33.0 31.0 34.0 30.0 34.0 2 31.618142502217946 33.0 31.0 34.0 30.0 34.0 3 31.635698073260915 33.0 31.0 34.0 28.0 34.0 4 35.17335792065128 37.0 35.0 37.0 33.0 37.0 5 35.21287179999133 37.0 35.0 37.0 33.0 37.0 6 35.272104381829244 37.0 35.0 37.0 33.0 37.0 7 35.18590837770792 37.0 35.0 37.0 32.0 37.0 8 35.15603930705956 37.0 35.0 37.0 32.0 37.0 9 36.726094515508656 39.0 37.0 39.0 32.0 39.0 10-11 36.608670985144016 39.0 37.0 39.0 32.0 39.0 12-13 36.49840006630288 39.0 37.0 39.0 32.0 39.0 14-15 37.65092174188214 40.0 37.0 41.0 32.0 41.0 16-17 37.54620764479739 40.0 37.0 41.0 32.0 41.0 18-19 37.510933759279105 40.0 37.0 41.0 32.0 41.0 20-21 37.409872913201625 40.0 37.0 41.0 32.0 41.0 22-23 37.42765527023133 40.0 37.0 41.0 32.0 41.0 24-25 37.37422626234468 40.0 37.0 41.0 31.0 41.0 26-27 37.23857923476682 40.0 37.0 41.0 31.0 41.0 28-29 37.069140530083985 40.0 37.0 41.0 30.5 41.0 30-31 36.84514962412671 39.5 36.0 41.0 30.0 41.0 32-33 36.65657199042376 39.0 36.0 41.0 30.0 41.0 34-35 36.376651403191204 39.0 35.0 41.0 30.0 41.0 36-37 36.16863647750882 39.0 35.0 41.0 29.5 41.0 38-39 36.005210050160386 39.0 35.0 41.0 29.0 41.0 40-41 35.88510539709209 39.0 35.0 41.0 28.0 41.0 42-43 35.87594637001151 39.0 35.0 41.0 27.5 41.0 44-45 35.686019510375836 39.0 35.0 41.0 27.0 41.0 46-47 35.36379674400119 39.0 35.0 41.0 25.5 41.0 48-49 35.06941534798652 39.0 35.0 41.0 24.0 41.0 50-51 33.938009256032714 38.0 33.5 40.0 23.0 40.5 52-53 34.002595795417946 38.0 33.5 39.5 23.0 40.5 54-55 34.50182879164885 38.0 34.0 40.0 23.0 41.0 56-57 34.40281391682379 38.0 34.0 40.0 23.0 41.0 58-59 34.20941350205407 38.0 34.0 40.0 22.0 41.0 60-61 33.90378717529012 38.0 34.0 40.0 21.5 41.0 62-63 33.51329090200171 37.0 33.0 40.0 19.5 41.0 64-65 33.12386979018529 37.0 33.0 40.0 18.0 41.0 66-67 32.7371885695342 36.0 32.0 39.0 16.0 41.0 68-69 32.32387807804527 36.0 32.0 39.0 13.0 41.0 70-71 31.90116839989678 35.0 31.0 38.5 9.5 40.0 72-73 31.478464749282818 35.0 31.0 38.0 7.5 40.0 74-75 31.017321062277617 35.0 31.0 37.0 6.0 39.0 76-77 30.553393219400373 35.0 30.0 36.5 2.0 39.0 78-79 30.121328619299035 34.5 30.0 36.0 2.0 39.0 80-81 29.66383629969655 34.0 29.5 36.0 2.0 37.5 82-83 29.248994249355334 34.0 29.0 35.0 2.0 37.0 84-85 28.896045183905727 34.0 29.0 35.0 2.0 37.0 86-87 28.46254037977235 34.0 29.0 35.0 2.0 36.0 88-89 28.010300491432425 34.0 27.5 35.0 2.0 36.0 90-91 27.5610587364404 34.0 26.5 35.0 2.0 35.5 92-93 27.109044315564034 34.0 26.0 35.0 2.0 35.0 94-95 26.60940898140317 33.0 25.0 35.0 2.0 35.0 96-97 26.010112884420142 33.0 24.0 35.0 2.0 35.0 98-99 25.25427436960465 33.0 19.0 35.0 2.0 35.0 100-101 22.793194536793386 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1999728231890039 1101 2 0.31860367345961293 1101 3 0.3786651162851662 1101 4 0.21586678586123753 1101 5 0.15166497781680022 1101 6 0.2911717725550744 1101 7 0.337045572838818 1101 8 0.4479276460912871 1101 9 0.524226135497301 1101 10-11 0.4450093161818316 1101 12-13 0.44853805493160337 1101 14-15 0.38431631174857017 1101 16-17 0.5065954493265394 1101 18-19 0.6852988430522302 1101 20-21 0.7459314433053095 1101 22-23 0.6852899334201794 1101 24-25 0.6359672277418937 1101 26-27 0.5859535768277055 1101 28-29 0.5459973621003442 1101 30-31 0.5489450647201011 1101 32-33 0.516091514903728 1101 34-35 0.5086259662552877 1101 36-37 0.5071549454225419 1101 38-39 0.575954267644434 1101 40-41 0.6523956129103183 1101 42-43 0.6166474222751432 1101 44-45 0.6713886042256263 1101 46-47 0.603168928487861 1101 48-49 0.5472608579892864 1101 50-51 0.4931409002175755 1101 52-53 0.6815892309937368 1101 54-55 0.690837335017136 1101 56-57 0.5079552168871757 1101 58-59 0.4928224537696835 1101 60-61 0.5757515483985642 1101 62-63 0.4887643259732002 1101 64-65 0.418285338629353 1101 66-67 0.44162242112540184 1101 68-69 0.5490310856696894 1101 70-71 0.5031740725177691 1101 72-73 0.5050663791251502 1101 74-75 0.39756701346760437 1101 76-77 0.34373045100191035 1101 78-79 0.20431132869750712 1101 80-81 0.3599556433852307 1101 82-83 0.3759414404742287 1101 84-85 0.2939526384453366 1101 86-87 0.12367622025504588 1101 88-89 0.3027449493754375 1101 90-91 0.23239786058130463 1101 92-93 0.13378311679933574 1101 94-95 0.07400084694556597 1101 96-97 0.06279346877232328 1101 98-99 -0.02090137537842196 1101 100-101 0.29145001156347305 1102 1 0.03133941437005916 1102 2 0.18784521971860713 1102 3 0.17415857583721106 1102 4 0.3243088639157108 1102 5 0.3048475326129605 1102 6 0.328776684364783 1102 7 0.2648341053127581 1102 8 0.25843084033822805 1102 9 0.3174690398549842 1102 10-11 0.24395779688920527 1102 12-13 0.2833002577489836 1102 14-15 0.4202619171520041 1102 16-17 0.4668436935344005 1102 18-19 0.49893446622596827 1102 20-21 0.37296475963807296 1102 22-23 0.42598942430833375 1102 24-25 0.4090280470327343 1102 26-27 0.3858869856174536 1102 28-29 0.347363605874385 1102 30-31 0.32318604607776535 1102 32-33 0.3872969325847535 1102 34-35 0.44525673711641645 1102 36-37 0.546347492695439 1102 38-39 0.4260525925879435 1102 40-41 0.4960434070071429 1102 42-43 0.564964425045936 1102 44-45 0.6620756877203746 1102 46-47 0.6848929255836609 1102 48-49 0.7614091838524004 1102 50-51 0.8255392756372757 1102 52-53 0.6158855142933604 1102 54-55 0.46600003973547643 1102 56-57 0.6463306424242816 1102 58-59 0.5642161281010161 1102 60-61 0.47674762853806385 1102 62-63 0.386195470048726 1102 64-65 0.3212094547193445 1102 66-67 0.3806682546205167 1102 68-69 0.5335569495381023 1102 70-71 0.4695990091013904 1102 72-73 0.3772786202886742 1102 74-75 0.3953364560720196 1102 76-77 0.3692507076043192 1102 78-79 0.3187610666387606 1102 80-81 0.2819183207781144 1102 82-83 0.2850642173247806 1102 84-85 0.2796506360672062 1102 86-87 0.32804788503822024 1102 88-89 0.3296627893679407 1102 90-91 0.26894303502487915 1102 92-93 0.075360715411108 1102 94-95 -0.15214097627564627 1102 96-97 -0.27392253358303975 1102 98-99 -0.4236117193785134 1102 100-101 0.0045812960906950195 1103 1 0.0871185463987203 1103 2 0.29588695134042453 1103 3 0.29715974815682245 1103 4 0.2901167297141072 1103 5 0.29863529045789505 1103 6 0.1488168115802253 1103 7 0.2096836310975334 1103 8 0.2565319327217921 1103 9 0.2718126773205469 1103 10-11 0.25115673190904175 1103 12-13 0.34808910945567106 1103 14-15 0.4071923585741146 1103 16-17 0.567714490495689 1103 18-19 0.6095993482605948 1103 20-21 0.5254315486535219 1103 22-23 0.6268640730957102 1103 24-25 0.5402485329484108 1103 26-27 0.5159915079739648 1103 28-29 0.59036415564794 1103 30-31 0.6767119024907942 1103 32-33 0.659067567286094 1103 34-35 0.5718482093156183 1103 36-37 0.7727771597725948 1103 38-39 0.7662590191962977 1103 40-41 0.7755719889779797 1103 42-43 0.7809768924546532 1103 44-45 0.8087066759596553 1103 46-47 0.6709306956521033 1103 48-49 0.6934464132438478 1103 50-51 0.7948667297479588 1103 52-53 0.6770236592539121 1103 54-55 0.6316903893050352 1103 56-57 0.7011454676622364 1103 58-59 0.698604421164994 1103 60-61 0.6668012044539964 1103 62-63 0.4229585915572045 1103 64-65 0.4667932721604089 1103 66-67 0.437360738471277 1103 68-69 0.4149885041521628 1103 70-71 0.4631844394768656 1103 72-73 0.5086434430819331 1103 74-75 0.4791683237215736 1103 76-77 0.47354188348296233 1103 78-79 0.5309184388917423 1103 80-81 0.5341836493402994 1103 82-83 0.6148041949017511 1103 84-85 0.6762563955563401 1103 86-87 0.5610539410753219 1103 88-89 0.6451056697720077 1103 90-91 0.562984615416326 1103 92-93 0.4829537021881194 1103 94-95 0.5442476454016969 1103 96-97 0.6075175490592386 1103 98-99 0.5393618562417899 1103 100-101 1.0332363237044504 1104 1 0.09346998729171929 1104 2 0.16733886355826755 1104 3 0.18299610631893515 1104 4 0.12301424706000574 1104 5 0.17666403668454933 1104 6 0.2552323027329493 1104 7 0.32001768859894497 1104 8 0.35147294156514874 1104 9 0.32567855400602497 1104 10-11 0.3320644659569183 1104 12-13 0.28757994206986837 1104 14-15 0.4461118455228572 1104 16-17 0.6383540361166098 1104 18-19 0.5504921372478648 1104 20-21 0.47476035006022244 1104 22-23 0.4186479134026513 1104 24-25 0.35850053448405106 1104 26-27 0.3375736892980399 1104 28-29 0.4083246571483201 1104 30-31 0.4932957711152852 1104 32-33 0.4261870908349934 1104 34-35 0.5684084109634071 1104 36-37 0.4794166169711218 1104 38-39 0.5242557179076854 1104 40-41 0.7360553012130637 1104 42-43 0.652305055317477 1104 44-45 0.5804060449148949 1104 46-47 0.6435503988439208 1104 48-49 0.6356055169991137 1104 50-51 0.8022138317884995 1104 52-53 0.8033480361839622 1104 54-55 0.6782261628721216 1104 56-57 0.6593696021582716 1104 58-59 0.7303821222727365 1104 60-61 0.6199798070222826 1104 62-63 0.7043557406630825 1104 64-65 0.7448442918718854 1104 66-67 0.5887369096480413 1104 68-69 0.5842078073166803 1104 70-71 0.5978102365374376 1104 72-73 0.4203217305755409 1104 74-75 0.3697774615586269 1104 76-77 0.5811473691400693 1104 78-79 0.3341699059756742 1104 80-81 0.471841396539606 1104 82-83 0.4949841092551637 1104 84-85 0.4967490369118259 1104 86-87 0.36391107513819776 1104 88-89 0.5598524375983516 1104 90-91 0.5060190587303097 1104 92-93 0.5637945598180174 1104 94-95 0.8048998135967267 1104 96-97 0.5892265689251737 1104 98-99 0.5514678869836764 1104 100-101 0.8135338594063874 1105 1 -0.0921760797191098 1105 2 0.1492819208956142 1105 3 0.06727986827694465 1105 4 0.09856620128513072 1105 5 0.20415641932156348 1105 6 0.2711808883641922 1105 7 0.11561248813136871 1105 8 0.0774500884465752 1105 9 0.08607320857002776 1105 10-11 0.20348488879520943 1105 12-13 0.18152613764408443 1105 14-15 0.272171909948689 1105 16-17 0.33180910997705126 1105 18-19 0.35995668073119447 1105 20-21 0.3696209037544804 1105 22-23 0.24789069363890093 1105 24-25 0.28549184255636817 1105 26-27 0.2558491141989734 1105 28-29 0.32830133315693644 1105 30-31 0.4572027524567801 1105 32-33 0.3429343069539428 1105 34-35 0.37856182901266067 1105 36-37 0.3869982869771391 1105 38-39 0.39570483241282517 1105 40-41 0.4474109550158403 1105 42-43 0.25740311149136375 1105 44-45 0.39792718099507596 1105 46-47 0.454626960978203 1105 48-49 0.550681203282501 1105 50-51 0.5453140693527558 1105 52-53 0.5286732370084692 1105 54-55 0.43379622442192556 1105 56-57 0.26236836182712153 1105 58-59 0.3536831877769089 1105 60-61 0.4368949860809366 1105 62-63 0.3744544935365397 1105 64-65 0.20411139138460754 1105 66-67 0.31168962536921896 1105 68-69 0.3254040301754273 1105 70-71 0.18475566539892085 1105 72-73 0.2625093946688821 1105 74-75 0.31422153992119917 1105 76-77 0.14242782743796667 1105 78-79 0.17018000708977254 1105 80-81 0.14862626875529372 1105 82-83 0.2444864323878555 1105 84-85 0.1850277359575152 1105 86-87 0.29788345014240036 1105 88-89 0.31428361843890684 1105 90-91 0.34065703922134816 1105 92-93 0.35030268100118533 1105 94-95 0.27242091535673296 1105 96-97 0.2605889906665908 1105 98-99 0.25035351087985447 1105 100-101 0.7515256074633463 1106 1 0.11262945407347758 1106 2 0.1630341315845918 1106 3 0.13666512421620425 1106 4 0.1903741693727241 1106 5 0.24526055275178038 1106 6 0.2311428922531107 1106 7 0.25070463005387467 1106 8 0.11573279412691306 1106 9 0.14967501798597027 1106 10-11 0.15337406680455246 1106 12-13 0.23349508854276735 1106 14-15 0.4142289230592482 1106 16-17 0.36893461216033785 1106 18-19 0.3025029884915753 1106 20-21 0.39728181552582953 1106 22-23 0.3026471761841023 1106 24-25 0.2952654896553071 1106 26-27 0.2272755785985936 1106 28-29 0.25588883719943567 1106 30-31 0.12550888095345414 1106 32-33 0.31490378557639787 1106 34-35 0.31351879246305714 1106 36-37 0.35755407482135837 1106 38-39 0.440693418694984 1106 40-41 0.5306214910884606 1106 42-43 0.44193363354548154 1106 44-45 0.5878594970393678 1106 46-47 0.5236172359764026 1106 48-49 0.621114667464262 1106 50-51 0.5576632790525906 1106 52-53 0.4195087049167725 1106 54-55 0.2615120735903531 1106 56-57 0.3979065656448668 1106 58-59 0.4918882910010325 1106 60-61 0.5692710420358864 1106 62-63 0.3272537298255216 1106 64-65 0.30217227717570694 1106 66-67 0.4130027103476337 1106 68-69 0.4661484995627134 1106 70-71 0.44084519170594305 1106 72-73 0.405988758639964 1106 74-75 0.3977895073592883 1106 76-77 0.4151467414636443 1106 78-79 0.4657704014347388 1106 80-81 0.566680510639511 1106 82-83 0.47452068030706585 1106 84-85 0.6200601368435521 1106 86-87 0.7620634553655137 1106 88-89 0.8450051927336197 1106 90-91 0.8410282725929719 1106 92-93 0.9478976137705288 1106 94-95 0.9874226812016396 1106 96-97 0.7278850694083907 1106 98-99 0.8621482723419476 1106 100-101 1.481537122666527 1107 1 -0.003145854070865539 1107 2 0.027936225236349088 1107 3 0.1492813081617541 1107 4 0.17780003937365763 1107 5 0.14582994731777887 1107 6 0.04517267067906516 1107 7 -0.012721819709639703 1107 8 -0.03815476261285511 1107 9 0.017000900100555327 1107 10-11 -0.06401508137292211 1107 12-13 -0.05348633207657372 1107 14-15 0.11400513787609867 1107 16-17 0.19933990640836896 1107 18-19 0.14937332850433904 1107 20-21 0.12794956342855812 1107 22-23 0.22752507426966417 1107 24-25 0.1547988283842372 1107 26-27 0.033208914365232545 1107 28-29 0.09775369961955249 1107 30-31 0.08937105192099182 1107 32-33 0.17583865803127452 1107 34-35 0.22578130007375563 1107 36-37 0.20455613096840608 1107 38-39 0.08077696586867233 1107 40-41 -0.06483104904925341 1107 42-43 0.07368415154959962 1107 44-45 0.255184876622522 1107 46-47 0.21115099154194183 1107 48-49 0.09944974378821314 1107 50-51 0.08078237001024746 1107 52-53 0.04763914515017831 1107 54-55 0.018347294590455476 1107 56-57 0.21374162213204073 1107 58-59 0.268999465237151 1107 60-61 0.15465906771683535 1107 62-63 0.1128186730209606 1107 64-65 0.1809996491637662 1107 66-67 -0.03821391825889009 1107 68-69 0.053070700819674244 1107 70-71 0.18572082575515125 1107 72-73 0.1314645633382625 1107 74-75 0.25689542987740666 1107 76-77 0.1665703601237496 1107 78-79 0.1364771563565057 1107 80-81 0.02227379131813123 1107 82-83 0.019100905218977005 1107 84-85 0.20605860597410341 1107 86-87 0.08027429533887442 1107 88-89 0.08016451371313948 1107 90-91 0.2737364039218946 1107 92-93 0.29896382678355593 1107 94-95 0.22350560206245973 1107 96-97 0.3197392199742133 1107 98-99 0.44770702646600924 1107 100-101 0.9971419139599327 1108 1 0.1857805727079196 1108 2 0.25292434234660277 1108 3 0.13323535359236516 1108 4 0.24837501763458647 1108 5 0.14444410796762241 1108 6 0.052969581497514184 1108 7 0.05157065838559305 1108 8 0.21185113972017433 1108 9 0.1834464070597619 1108 10-11 0.2742590024925491 1108 12-13 0.28741059213945874 1108 14-15 0.3236495358224758 1108 16-17 0.47617703245289533 1108 18-19 0.3862064324289278 1108 20-21 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-1.145768354563284 2316 96-97 -1.2719191033365966 2316 98-99 -1.6527384072870461 2316 100-101 -1.610384702569636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13799.0 3 3323.0 4 3140.0 5 3439.0 6 3994.0 7 4772.0 8 5714.0 9 6996.0 10 8390.0 11 10097.0 12 12445.0 13 15269.0 14 16228.0 15 18535.0 16 21906.0 17 27522.0 18 24608.0 19 18023.0 20 18257.0 21 19440.0 22 20854.0 23 23439.0 24 26256.0 25 30041.0 26 34568.0 27 39970.0 28 46783.0 29 55271.0 30 65947.0 31 79124.0 32 97131.0 33 122677.0 34 161167.0 35 226059.0 36 331560.0 37 495966.0 38 461861.0 39 79872.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.796126759765265 18.715787851244727 14.166634555885228 22.32145083310478 2 15.167095406701778 20.621483743664655 40.92330867810837 23.288112171525196 3 19.895178276197882 25.399960743754864 29.717128556653556 24.9877324233937 4 12.576195180411922 16.5114022026322 37.68696450174371 33.22543811521217 5 13.357707453163778 37.958374179242675 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21.973487287060774 26.402422865939062 29.905791296780666 21.718298550219505 28-29 21.594076077313478 26.825617246917094 29.940483760061326 21.639822915708095 30-31 21.770128435625107 26.765510480934147 29.681556650279806 21.782804433160937 32-33 21.62894735451473 26.790733120296412 29.534697011217276 22.045622513971583 34-35 22.16548694683264 26.664989184588688 29.437655326207246 21.731868542371423 36-37 22.26052565983597 26.419764573808695 29.608960734941853 21.710749031413485 38-39 22.17450783707358 26.552718134796343 29.685959257995197 21.586814770134882 40-41 22.040889309595023 26.62604223056242 29.705102053160186 21.627966406682372 42-43 21.71440564359225 26.864089032924106 29.285985747320296 22.135519576163347 44-45 21.95092987640286 26.966330590911298 29.01795650132526 22.064783031360577 46-47 22.104491021304508 27.0564125799503 28.47956243124931 22.359533967495874 48-49 22.225914639220807 27.00599117249644 28.498356002293878 22.26973818598887 50-51 22.210195963917947 27.349898931518542 28.505964250807185 21.93394085375633 52-53 22.17238787650463 27.386214098940183 28.43144931137823 22.009948713176957 54-55 21.83011213108442 27.218580631105887 28.583942483767917 22.367364754041784 56-57 22.053309222532558 27.629480218630814 28.066526297638305 22.250684261198327 58-59 22.19680023167112 27.14224901696666 28.29812837105487 22.36282238030735 60-61 22.070720804052463 27.025281401543804 28.73535547004692 22.168642324356817 62-63 22.19045893806588 26.96134918026649 28.669910479980242 22.178281401687382 64-65 21.994965458459927 27.134922913784422 28.59956668440938 22.270544943346277 66-67 21.863451533137006 27.28498854422353 28.294557219863137 22.557002702776323 68-69 21.854836573858627 27.33075680358974 28.396214279498615 22.41819234305302 70-71 21.956294403452922 27.1946060185525 28.1651541715883 22.68394540640628 72-73 21.947421643373456 27.055276089146613 28.13885207717081 22.85845019030912 74-75 21.899706253416483 27.476711173151603 28.010183719289483 22.61339885414243 76-77 22.16823148196828 27.07104625834336 28.079057623505555 22.681664636182806 78-79 22.019128490166707 27.143900803808002 28.258065589553105 22.578905116472185 80-81 22.02648119781254 27.37585484901504 27.9678177602866 22.629846192885818 82-83 22.090634333678647 27.317664572480876 27.92608284382639 22.665618250014088 84-85 22.096472746754454 27.12298685745051 27.937633168612575 22.84290722718246 86-87 22.107814614669962 27.055313833214168 28.135358544020683 22.70151300809518 88-89 22.31197462364477 26.873890006670127 27.98424738504436 22.829887984640745 90-91 22.114911176454456 27.01957116145428 28.110895366686183 22.754622295405085 92-93 22.215948577042695 27.15361628671075 27.9362834724191 22.694151663827448 94-95 22.1188848110085 27.090809105824302 27.868355654478993 22.921950428688202 96-97 22.109799498656784 26.78043335107135 27.916712934946265 23.193054215325606 98-99 22.226179979770667 26.834415894572906 27.62073803581768 23.31866608983874 100-101 22.582944434032612 26.423076421194725 28.01283410243264 22.981145042340017 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3241.0 1 2146.0 2 1147.5 3 1277.0 4 1129.0 5 1043.0 6 1511.5 7 2749.0 8 4237.0 9 4868.0 10 4326.5 11 3661.5 12 3590.0 13 3684.0 14 3963.0 15 4191.0 16 4326.5 17 4547.0 18 4772.0 19 5136.5 20 5617.5 21 6420.0 22 7543.5 23 9016.0 24 10692.0 25 12742.5 26 16016.0 27 20319.0 28 23988.0 29 27754.0 30 32047.0 31 37158.0 32 42538.5 33 46921.0 34 52138.5 35 57072.0 36 63021.5 37 68717.0 38 71869.5 39 74118.0 40 76197.0 41 79475.5 42 82074.0 43 84690.0 44 88683.0 45 91515.0 46 93640.5 47 99550.0 48 117407.5 49 118385.5 50 102680.0 51 96525.0 52 91087.5 53 87866.5 54 84600.5 55 77858.5 56 70242.0 57 62376.0 58 56131.5 59 50526.5 60 43307.5 61 35651.5 62 28513.5 63 21831.0 64 15792.5 65 11641.5 66 8644.5 67 6619.5 68 5154.0 69 3947.5 70 3066.5 71 2219.5 72 1654.0 73 1347.5 74 1054.0 75 829.0 76 613.0 77 392.0 78 250.5 79 183.5 80 145.0 81 111.0 82 81.0 83 69.0 84 55.0 85 34.0 86 29.0 87 23.5 88 17.5 89 13.5 90 10.5 91 8.5 92 7.0 93 4.0 94 2.5 95 2.0 96 3.5 97 4.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2787357999762666 2 0.16846959014648794 3 0.19683667453385495 4 0.1651167740635189 5 0.23812528607243966 6 0.19845657443910966 7 0.24509462287411682 8 0.24991665049906103 9 0.15370213054509632 10-11 0.20904243195949496 12-13 0.21501345835444538 14-15 0.1761923687645626 16-17 0.28885075636140345 18-19 0.2349043223073402 20-21 0.2020730951578178 22-23 0.24741145645953924 24-25 0.2386903674347378 26-27 0.3461311704530257 28-29 0.31646439893237294 30-31 0.2919775398994532 32-33 0.19510375835614066 34-35 0.36346033223016894 36-37 0.40248861831956106 38-39 0.38284262295699545 40-41 0.3464702192704046 42-43 0.37935795455615684 44-45 0.38762697849111977 46-47 0.39256202238852356 48-49 0.41171828057043075 50-51 0.40433455076973496 52-53 0.4040708461339959 54-55 0.39821283601150503 56-57 0.37773805465090216 58-59 0.352460081710765 60-61 0.3197795429245221 62-63 0.38696771690177195 64-65 0.419893124278344 66-67 0.4248281681757478 68-69 0.4292734748924933 70-71 0.4291227865292137 72-73 0.4201945010049031 74-75 0.41627660355963586 76-77 0.39992691614380943 78-79 0.3747619594761319 80-81 0.3854419972235669 82-83 0.395481609427064 84-85 0.3961408710164118 86-87 0.3910928108465484 88-89 0.3995690312810206 90-91 0.4212681555932695 92-93 0.4201191568232633 94-95 0.4280868040316671 96-97 0.4289155900297045 98-99 0.4254497576742758 100-101 0.39900394991872246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2654485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.779952551070124 #Duplication Level Percentage of deduplicated Percentage of total 1 76.81698307703897 44.38481637307668 2 13.4612793816224 15.555841678936819 3 4.35598544849983 7.5506589758241605 4 1.9131604594571756 4.421692822800765 5 0.9658581607011628 2.790361934818852 6 0.6029344622006731 2.0902514770415928 7 0.37385958665775876 1.5121112424493592 8 0.2574277358943342 1.1899329892243233 9 0.18898108230630672 0.9827386171837452 >10 0.9559640260176886 9.998703654962805 >50 0.07237958473186722 2.8627900792157783 >100 0.032115002938576075 3.436292935870772 >500 0.0018301228538011665 0.7757863229347289 >1k 9.804229573850457E-4 1.2306383564888095 >5k 1.9608459147700912E-4 0.7764661084333739 >10k+ 6.536153049233638E-5 0.44091643073733666 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11675 0.4398216603220587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9378 0.3532888677088023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5894 0.22203930329235239 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5288 0.1992100162555072 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4939 0.18606245655937026 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4241 0.15976733716709643 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3221 0.12134180453082237 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 0.10269411957498348 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.767209081987655E-5 0.0 4 0.0 0.0 0.0 3.767209081987655E-5 0.0 5 0.0 0.0 0.0 3.767209081987655E-5 0.0 6 3.767209081987655E-5 0.0 0.0 7.53441816397531E-5 0.0 7 3.767209081987655E-5 0.0 0.0 7.53441816397531E-5 0.0 8 3.767209081987655E-5 0.0 0.0 7.53441816397531E-5 0.0 9 3.767209081987655E-5 0.0 0.0 1.1301627245962965E-4 0.0 10-11 3.767209081987655E-5 0.0 0.0 1.8836045409938276E-4 3.767209081987655E-5 12-13 3.767209081987655E-5 0.0 0.0 2.260325449192593E-4 3.767209081987655E-5 14-15 3.767209081987655E-5 0.0 0.0 2.260325449192593E-4 3.767209081987655E-5 16-17 3.767209081987655E-5 0.0 0.0 3.013767265590124E-4 3.767209081987655E-5 18-19 3.767209081987655E-5 0.0 0.0 4.1439299901864204E-4 3.767209081987655E-5 20-21 3.767209081987655E-5 0.0 0.0 5.839174077080865E-4 3.767209081987655E-5 22-23 3.767209081987655E-5 0.0 0.0 7.722778618074693E-4 3.767209081987655E-5 24-25 3.767209081987655E-5 0.0 0.0 0.0013938673603354323 3.767209081987655E-5 26-27 1.1301627245962965E-4 0.0 0.0 0.0021661452221429017 3.767209081987655E-5 28-29 1.1301627245962965E-4 0.0 0.0 0.005424781078062223 3.767209081987655E-5 30-31 1.506883632795062E-4 0.0 0.0 0.015389049099919571 3.767209081987655E-5 32-33 1.506883632795062E-4 0.0 0.0 0.035976846732982105 3.767209081987655E-5 34-35 1.506883632795062E-4 0.0 0.0 0.06892109015496414 3.767209081987655E-5 36-37 1.8836045409938274E-4 0.0 0.0 0.12094624757721366 3.767209081987655E-5 38-39 1.8836045409938274E-4 0.0 0.0 0.1939924316769543 3.767209081987655E-5 40-41 1.8836045409938274E-4 0.0 0.0 0.27700288379855226 7.53441816397531E-5 42-43 1.8836045409938274E-4 0.0 0.0 0.35869481274145454 7.53441816397531E-5 44-45 2.0719649950932102E-4 0.0 0.0 0.458036116233469 7.53441816397531E-5 46-47 2.260325449192593E-4 0.0 0.0 0.5850475704326827 7.53441816397531E-5 48-49 2.260325449192593E-4 0.0 0.0 0.7239445692855676 7.53441816397531E-5 50-51 2.260325449192593E-4 0.0 0.0 0.8686242340793036 7.53441816397531E-5 52-53 2.260325449192593E-4 0.0 0.0 1.0116463268769649 7.53441816397531E-5 54-55 2.260325449192593E-4 0.0 0.0 1.1724873186324278 7.53441816397531E-5 56-57 2.260325449192593E-4 0.0 0.0 1.3676475851247982 7.53441816397531E-5 58-59 2.260325449192593E-4 0.0 0.0 1.5847330084743367 7.53441816397531E-5 60-61 2.6370463573913584E-4 0.0 0.0 1.8267761919920438 7.53441816397531E-5 62-63 2.825406811490741E-4 0.0 0.0 2.0609459085283963 7.53441816397531E-5 64-65 3.013767265590124E-4 0.0 0.0 2.3022733223205254 7.53441816397531E-5 66-67 3.3904881737888896E-4 0.0 0.0 2.574284654085444 7.53441816397531E-5 68-69 3.3904881737888896E-4 0.0 0.0 2.888959628703873 7.53441816397531E-5 70-71 3.3904881737888896E-4 0.0 0.0 3.2361456176998553 7.53441816397531E-5 72-73 3.7672090819876547E-4 0.0 0.0 3.5888882400917694 7.53441816397531E-5 74-75 3.9555695360870375E-4 0.0 0.0 3.9370160313582483 7.53441816397531E-5 76-77 4.1439299901864204E-4 0.0 0.0 4.318370606727859 7.53441816397531E-5 78-79 4.1439299901864204E-4 0.0 0.0 4.758041578686639 7.53441816397531E-5 80-81 4.1439299901864204E-4 0.0 0.0 5.231108859157238 7.53441816397531E-5 82-83 4.1439299901864204E-4 0.0 0.0 5.7040442873099675 7.53441816397531E-5 84-85 4.1439299901864204E-4 0.0 0.0 6.190014258886375 7.53441816397531E-5 86-87 4.1439299901864204E-4 0.0 0.0 6.697494994320932 7.53441816397531E-5 88-89 4.1439299901864204E-4 0.0 0.0 7.243212901937664 7.53441816397531E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2665 0.0 28.304956 2 GTACATG 15645 0.0 23.360668 1 TACATGG 15505 0.0 23.193167 2 GTACCTG 3380 0.0 22.216461 1 GTACCGA 150 3.0652667E-5 22.138327 6 ACATGGG 15680 0.0 21.875343 3 GAGTACT 9905 0.0 21.395903 12-13 CATGGGG 5565 0.0 20.886396 4 AGTACTT 10300 0.0 19.791994 12-13 GTACTTT 10580 0.0 19.739624 14-15 ACCTGGG 3655 0.0 19.729624 3 GTATCAA 23950 0.0 19.645548 1 GGTATCA 19045 0.0 18.765984 1 CATGGGA 10105 0.0 18.12231 4 GTACACG 460 0.0 17.564087 1 TATCAAC 27290 0.0 17.140993 2 ATCAACG 27170 0.0 17.11193 3 ACTTTTT 12670 0.0 17.083002 16-17 TAGACCG 250 4.618216E-6 17.079103 5 TCAACGC 27335 0.0 17.043352 4 >>END_MODULE