FastQCFastQC Report
Thu 26 May 2016
SRR938740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654485
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113300.4268247889892013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98720.3718988805738213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63160.23793692561834026No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA54500.20531289496832722No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA53610.20196007888535816No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT48330.18206921493246336No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28550.10755381929074753No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.10729011465500841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25600.024.8420812
GTACATG148200.022.4067571
TACATGG147200.022.1499162
GAGTACT106550.021.68623212-13
ACATGGG149550.021.103693
GTACTTT112800.020.48464614-15
AGTACTT110000.019.90616612-13
GTACCTG33050.019.576691
CATGGGA97050.019.0718234
GTATCAA256450.018.3284631
GTGTAGC16900.018.0162771
ACTTTTT135500.017.50794816-17
ACCTGGG36450.017.4473883
AGTACAT115700.017.2691862
CATGGGG54000.017.0502644
GGTATCA207250.017.032611
TACTTTT128650.016.89134814-15
ATCAACG289900.016.0599423
TCAACGC290750.016.0456374
TATCAAC291250.016.0343842
Produced by FastQC (version 0.11.5)