##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938740_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2654485 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.127318481739394 33.0 31.0 34.0 30.0 34.0 2 32.324601947270374 34.0 31.0 34.0 30.0 34.0 3 32.40243926788059 34.0 31.0 34.0 30.0 34.0 4 35.99884309009092 37.0 35.0 37.0 35.0 37.0 5 35.91127996579374 37.0 35.0 37.0 35.0 37.0 6 35.94462541698296 37.0 35.0 37.0 35.0 37.0 7 35.87014844687388 37.0 35.0 37.0 35.0 37.0 8 35.858066253906124 37.0 35.0 37.0 35.0 37.0 9 37.514383015914575 39.0 37.0 39.0 35.0 39.0 10-11 37.44609199147857 39.0 37.0 39.0 34.0 39.0 12-13 37.37005897565818 39.0 37.0 39.0 34.0 39.0 14-15 38.59870690548261 40.0 38.0 41.0 34.0 41.0 16-17 38.52982235725574 40.0 38.0 41.0 34.0 41.0 18-19 38.68726834018652 40.0 38.0 41.0 34.0 41.0 20-21 38.695337702040135 40.0 38.0 41.0 34.0 41.0 22-23 38.62344805112856 40.0 38.0 41.0 34.0 41.0 24-25 38.552331431520614 40.0 38.0 41.0 34.0 41.0 26-27 38.375895324328454 40.0 38.0 41.0 34.0 41.0 28-29 38.21288121801404 40.0 38.0 41.0 33.0 41.0 30-31 37.998539076318 40.0 37.0 41.0 33.0 41.0 32-33 37.84978027753029 40.0 37.0 41.0 33.0 41.0 34-35 37.82335161057607 40.0 37.0 41.0 33.0 41.0 36-37 37.71192378182586 40.0 37.0 41.0 33.0 41.0 38-39 37.59184323889568 40.0 37.0 41.0 33.0 41.0 40-41 37.418548795717435 40.0 37.0 41.0 32.0 41.0 42-43 37.226153660691246 40.0 36.0 41.0 31.0 41.0 44-45 36.976454754877125 40.0 36.0 41.0 31.0 41.0 46-47 36.64880004972716 40.0 36.0 41.0 30.0 41.0 48-49 36.334965162734015 39.0 35.5 41.0 30.0 41.0 50-51 36.10172896060818 39.0 35.0 41.0 29.0 41.0 52-53 35.88102682817948 39.0 35.0 41.0 28.5 41.0 54-55 35.618125549777076 39.0 35.0 40.0 28.0 41.0 56-57 35.3632710299738 38.0 35.0 40.0 27.5 41.0 58-59 35.07642989129718 38.0 34.0 40.0 27.0 41.0 60-61 34.723338802065186 38.0 34.0 40.0 26.0 41.0 62-63 34.36349046990283 37.0 34.0 40.0 26.0 41.0 64-65 33.9670845757275 37.0 33.0 39.5 25.5 41.0 66-67 33.57415129488395 36.0 33.0 39.0 25.0 40.5 68-69 33.24107218537682 36.0 33.0 39.0 23.5 40.0 70-71 32.811381492078496 35.0 32.0 38.5 23.0 40.0 72-73 32.329462965509315 35.0 32.0 37.5 22.0 40.0 74-75 31.842071814306728 35.0 31.0 37.0 20.5 39.0 76-77 29.881900820686496 33.0 29.0 35.0 18.0 37.5 78-79 30.829366336596365 34.0 30.5 36.0 18.0 38.0 80-81 30.886895198126943 34.0 31.0 36.0 18.0 37.5 82-83 30.56599999623279 34.0 31.0 35.0 17.0 37.0 84-85 30.17620009154318 34.0 30.5 35.0 10.5 36.5 86-87 29.601993230325277 34.0 30.0 35.0 4.5 36.0 88-89 29.005480912493383 34.0 29.0 35.0 2.0 36.0 90-91 28.490111076159785 34.0 29.0 35.0 2.0 35.0 92-93 28.207941465105286 34.0 29.0 35.0 2.0 35.0 94-95 27.88727643968604 33.5 28.0 35.0 2.0 35.0 96-97 27.298241655160982 33.0 27.0 35.0 2.0 35.0 98-99 26.495231655104476 33.0 25.5 35.0 2.0 35.0 100-101 22.85036136953119 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14557111462754335 1101 2 0.158857083707602 1101 3 0.06710355980172977 1101 4 0.12783307889664286 1101 5 0.04779059104875927 1101 6 0.11743589714702551 1101 7 0.21037373532130488 1101 8 0.17788274213422284 1101 9 0.2212809197849097 1101 10-11 0.2551254541117913 1101 12-13 0.18753682768225133 1101 14-15 0.3022164772640039 1101 16-17 0.2617110591135656 1101 18-19 0.24001899376507652 1101 20-21 0.21836206762137778 1101 22-23 0.12595584925744419 1101 24-25 0.18263021977584515 1101 26-27 0.020257925242333386 1101 28-29 0.14927379602808344 1101 30-31 0.10769592375049797 1101 32-33 0.11444162759652698 1101 34-35 0.2808168284773487 1101 36-37 0.3127624138146885 1101 38-39 0.3729072569785572 1101 40-41 0.35421487563763066 1101 42-43 0.43053764728141886 1101 44-45 0.374686453606941 1101 46-47 0.23565248580747067 1101 48-49 0.22001113323562294 1101 50-51 0.19983857056970322 1101 52-53 0.09098407155313026 1101 54-55 0.3745458426971382 1101 56-57 0.32213147695892275 1101 58-59 0.34697452314030386 1101 60-61 0.3403669862338603 1101 62-63 0.2594078966308402 1101 64-65 0.13227437215444127 1101 66-67 0.10477353007765089 1101 68-69 0.13818214237145554 1101 70-71 0.2993081930313153 1101 72-73 0.19106774894334677 1101 74-75 0.1931504832816131 1101 76-77 0.14092584167030608 1101 78-79 0.021031779880811285 1101 80-81 0.19972612642373377 1101 82-83 0.2031935746432758 1101 84-85 0.38638888773515134 1101 86-87 0.30933337956975393 1101 88-89 0.4831291666065418 1101 90-91 0.3932374297832659 1101 92-93 0.46722616864882127 1101 94-95 0.35069938925695965 1101 96-97 0.3261245892384501 1101 98-99 0.09676807751726457 1101 100-101 0.6051512230881997 1102 1 0.21516272454376661 1102 2 0.1776342869176375 1102 3 0.13000933630457467 1102 4 0.08183974735970878 1102 5 -0.09940736701541653 1102 6 -0.03530480847457085 1102 7 0.0984593387453927 1102 8 0.20992607634239846 1102 9 0.15256585572042525 1102 10-11 0.1904253495685495 1102 12-13 0.2338327536489473 1102 14-15 0.2927697798639812 1102 16-17 0.2398654345297615 1102 18-19 0.22903159004280127 1102 20-21 0.27213935987271753 1102 22-23 0.19262807249010194 1102 24-25 0.2583542933595666 1102 26-27 0.276013277134993 1102 28-29 0.2866390407880104 1102 30-31 0.2597507924554634 1102 32-33 0.29648662585510976 1102 34-35 0.37598861540766393 1102 36-37 0.47685252710733295 1102 38-39 0.4246567432394812 1102 40-41 0.5098801498541619 1102 42-43 0.5618115187414148 1102 44-45 0.44648103271458695 1102 46-47 0.3986474641704376 1102 48-49 0.33540689843169247 1102 50-51 0.2962342373824427 1102 52-53 0.1990081664191763 1102 54-55 0.2614223091415653 1102 56-57 0.2578957738523755 1102 58-59 0.33930230195122135 1102 60-61 0.3489301312541002 1102 62-63 0.2523744372481218 1102 64-65 0.29628675677663097 1102 66-67 0.36169627172039753 1102 68-69 0.33524629034699416 1102 70-71 0.20969805668720198 1102 72-73 0.2045842903342603 1102 74-75 0.18840421739613333 1102 76-77 0.1268088554232456 1102 78-79 0.11516915512447312 1102 80-81 0.40499980870746555 1102 82-83 0.4818565510105479 1102 84-85 0.49919588678956117 1102 86-87 0.6232531320524721 1102 88-89 0.5450066621347709 1102 90-91 0.5513560835840003 1102 92-93 0.5970019568321376 1102 94-95 0.6256453295140467 1102 96-97 0.6082753568765789 1102 98-99 0.7894538753141198 1102 100-101 1.3186573568655895 1103 1 0.217766189424772 1103 2 0.19231614029695976 1103 3 0.18269833730860796 1103 4 0.06340598796608532 1103 5 0.10141746387377992 1103 6 0.05623234587022807 1103 7 0.12073397991439094 1103 8 0.06056476880237227 1103 9 0.16015580083404046 1103 10-11 0.23243434550125386 1103 12-13 0.1616137862908289 1103 14-15 0.16306074481605748 1103 16-17 0.09358601425374502 1103 18-19 0.01787071788704253 1103 20-21 0.10500116431251882 1103 22-23 0.18874092616164972 1103 24-25 0.16615451122226688 1103 26-27 0.15516138112690214 1103 28-29 0.15297733572815986 1103 30-31 0.2482491118653698 1103 32-33 0.26418572404455176 1103 34-35 0.28619913831224153 1103 36-37 0.3918974470746335 1103 38-39 0.3306769484124672 1103 40-41 0.3796712302188965 1103 42-43 0.29291944889799737 1103 44-45 0.46988535335479753 1103 46-47 0.46506978861528836 1103 48-49 0.4804035611299824 1103 50-51 0.5656551871091438 1103 52-53 0.5916183314876946 1103 54-55 0.45773822937835007 1103 56-57 0.46454446627823387 1103 58-59 0.47960691459112326 1103 60-61 0.5988015001618194 1103 62-63 0.6068036250500839 1103 64-65 0.7007042611799363 1103 66-67 0.6248186330063348 1103 68-69 0.4633949804987125 1103 70-71 0.40903979240246713 1103 72-73 0.567687477770086 1103 74-75 0.6167540695201268 1103 76-77 0.7629603183212055 1103 78-79 0.40874403917171165 1103 80-81 0.614515482086258 1103 82-83 0.6660587624988246 1103 84-85 0.752352962835694 1103 86-87 0.840699942740482 1103 88-89 0.5896901898502982 1103 90-91 0.5272775512462893 1103 92-93 0.5652722197114528 1103 94-95 0.7415870000751816 1103 96-97 0.9153937446699025 1103 98-99 1.0050393017098465 1103 100-101 1.628347655835519 1104 1 0.14854839727556168 1104 2 0.15538021070355512 1104 3 0.1296085348710605 1104 4 0.07854174429640182 1104 5 -8.984189117242636E-4 1104 6 0.061373295616100165 1104 7 0.10390854471400246 1104 8 -0.01574178374346502 1104 9 0.1790972170918934 1104 10-11 0.11623524013423747 1104 12-13 0.09955030182239 1104 14-15 0.1707311061949568 1104 16-17 0.21276384844276208 1104 18-19 0.2298439891456283 1104 20-21 0.22236123310288747 1104 22-23 0.17572737683146755 1104 24-25 0.19670122454012784 1104 26-27 0.23269906137144147 1104 28-29 0.11218894670550839 1104 30-31 0.03309179558524278 1104 32-33 0.11799429407563622 1104 34-35 0.13204745588064526 1104 36-37 0.2236649208473196 1104 38-39 0.28187835947690587 1104 40-41 0.24508383813056867 1104 42-43 0.1900700238113373 1104 44-45 0.1647257397717823 1104 46-47 0.1775348393910079 1104 48-49 0.2833624089080615 1104 50-51 0.14702538977547164 1104 52-53 0.197993179126982 1104 54-55 0.15859931222787793 1104 56-57 0.014560261984485123 1104 58-59 0.23189337455089998 1104 60-61 0.24577026355809295 1104 62-63 0.1649621143257889 1104 64-65 0.0681428188206965 1104 66-67 0.11337325173239776 1104 68-69 0.1834175561865905 1104 70-71 0.16432295946815856 1104 72-73 0.24905737263700445 1104 74-75 0.39618927718399277 1104 76-77 0.34561348490560206 1104 78-79 0.2072546957470358 1104 80-81 0.17729865754647278 1104 82-83 0.26781997675072233 1104 84-85 0.24784258216057253 1104 86-87 0.32770772950600247 1104 88-89 0.4120690120194652 1104 90-91 0.3360779422075062 1104 92-93 0.41070535784399453 1104 94-95 0.31045599952561886 1104 96-97 0.6123892166607128 1104 98-99 1.0424870177822356 1104 100-101 1.806845922085369 1105 1 0.10100204959433512 1105 2 0.1259779789311679 1105 3 0.08925185214941678 1105 4 0.01783209916015238 1105 5 -0.08619888175361012 1105 6 -0.09682665235451537 1105 7 -0.010530433670460582 1105 8 0.06329318782913163 1105 9 0.03441790824531665 1105 10-11 0.023742435359061176 1105 12-13 0.006833660861531143 1105 14-15 -0.008228223710482041 1105 16-17 0.025862074844724248 1105 18-19 0.07625859471414742 1105 20-21 0.029334406394376344 1105 22-23 9.986949801898959E-4 1105 24-25 -0.06982884782738807 1105 26-27 -0.013749864372741172 1105 28-29 -0.04332386879128336 1105 30-31 -0.05235418480146592 1105 32-33 0.01654955196086405 1105 34-35 0.025075998597941407 1105 36-37 0.008156800350555216 1105 38-39 0.004298014229668468 1105 40-41 -0.07755464206223195 1105 42-43 0.018688657332951664 1105 44-45 0.10881323150919542 1105 46-47 -0.1943907077927136 1105 48-49 -0.0431252162661977 1105 50-51 0.031626476077669 1105 52-53 0.0801050917285977 1105 54-55 0.09342543592190111 1105 56-57 0.04179194856715185 1105 58-59 0.09716266094520876 1105 60-61 0.08648545483472247 1105 62-63 0.01457721355571806 1105 64-65 0.10131917167053217 1105 66-67 -0.10236186240620526 1105 68-69 0.0437933346539765 1105 70-71 -0.03378929358959937 1105 72-73 0.09911884007161831 1105 74-75 0.06854156143192824 1105 76-77 0.036735267303335206 1105 78-79 -0.20973790962791483 1105 80-81 -0.015496919818410504 1105 82-83 0.19005395935313985 1105 84-85 0.166501088862276 1105 86-87 0.18369182965502162 1105 88-89 -0.09595367224384077 1105 90-91 -0.1183251647293595 1105 92-93 0.03287234746351331 1105 94-95 0.1446024464607909 1105 96-97 0.26144359286063334 1105 98-99 0.3442965147239647 1105 100-101 1.0663810621700307 1106 1 0.1757062198299053 1106 2 0.10163692324007911 1106 3 0.10153672014405402 1106 4 0.040171052075017144 1106 5 0.11810829136549472 1106 6 0.07216497578155412 1106 7 0.1492294114223185 1106 8 0.13877027040978618 1106 9 0.09761567069655541 1106 10-11 0.18119091801792564 1106 12-13 0.13585975341886325 1106 14-15 0.09728317444628232 1106 16-17 0.12136627430806612 1106 18-19 0.026117530092342633 1106 20-21 0.08950114004137077 1106 22-23 0.15601362685645626 1106 24-25 0.17208080857031405 1106 26-27 0.2258997811727994 1106 28-29 0.14721059360028477 1106 30-31 0.18833785049378804 1106 32-33 0.14503120950061543 1106 34-35 0.1089753494977117 1106 36-37 0.08909501201649306 1106 38-39 0.2815500753969147 1106 40-41 0.3799964084463454 1106 42-43 0.2505297502353372 1106 44-45 0.34673464938117604 1106 46-47 0.2841276874399057 1106 48-49 0.223116700426516 1106 50-51 0.15355191006280933 1106 52-53 0.11465509896788717 1106 54-55 0.17967829949989778 1106 56-57 0.13288349781527842 1106 58-59 0.1958334736262799 1106 60-61 0.25736132743950435 1106 62-63 0.2962304232663726 1106 64-65 0.2551936789592091 1106 66-67 0.06480932171120202 1106 68-69 0.05330744991155001 1106 70-71 0.16052157359585806 1106 72-73 0.25410603262177744 1106 74-75 0.3409222586497407 1106 76-77 0.24334412792066473 1106 78-79 0.15615185293698275 1106 80-81 0.17047164949540417 1106 82-83 0.2600389936484184 1106 84-85 0.2607083056877322 1106 86-87 0.47477762806724755 1106 88-89 0.5445832257958685 1106 90-91 0.302446042662023 1106 92-93 0.5054329443596863 1106 94-95 0.579273694852251 1106 96-97 0.7048433246687118 1106 98-99 0.8876215533222229 1106 100-101 1.3595114518872649 1107 1 -0.015951599638583502 1107 2 -0.03072007086020534 1107 3 -0.04467374319521866 1107 4 0.03880390207890372 1107 5 -0.10847493392358132 1107 6 -0.042486513377788526 1107 7 -0.047680655022922735 1107 8 -0.054403871544238314 1107 9 -0.05621607860479827 1107 10-11 -0.11280438675152027 1107 12-13 -0.060703698580653054 1107 14-15 -0.0799279241985289 1107 16-17 -0.05417324608650631 1107 18-19 -0.04985940424373325 1107 20-21 -0.03241338306423103 1107 22-23 -0.09437358683947394 1107 24-25 -0.09185809101280284 1107 26-27 -0.11204467482104974 1107 28-29 -0.025788498140265403 1107 30-31 0.010451243802926058 1107 32-33 -0.08548657244199376 1107 34-35 -0.15315787528883362 1107 36-37 -0.06516058287757431 1107 38-39 -0.04569442836388049 1107 40-41 -0.17255621320725822 1107 42-43 -0.1801439222485257 1107 44-45 -0.0876954455866894 1107 46-47 -0.21230156221718488 1107 48-49 -0.13261604595010112 1107 50-51 -0.2281182029115456 1107 52-53 -0.31391719864286927 1107 54-55 -0.25825827468511164 1107 56-57 -0.22618473484514112 1107 58-59 -0.19504843730504717 1107 60-61 -0.0518813100782225 1107 62-63 -0.1027948773399956 1107 64-65 -0.18823529933089844 1107 66-67 -0.16054772687406427 1107 68-69 -0.1341847806879528 1107 70-71 -0.24915011997210712 1107 72-73 -0.29761397378946697 1107 74-75 -0.21807387397412725 1107 76-77 -0.3453727717883446 1107 78-79 -0.21547318561246698 1107 80-81 -0.17087560554564973 1107 82-83 -0.26765181092556745 1107 84-85 -0.2435462841434628 1107 86-87 -0.07872001717623789 1107 88-89 -0.19319163906284942 1107 90-91 0.08255785855143216 1107 92-93 0.31591392513177396 1107 94-95 0.23516264915838647 1107 96-97 0.30946613779736865 1107 98-99 0.5845329422186047 1107 100-101 1.3130003160551347 1108 1 0.145059494187457 1108 2 0.11159456530434397 1108 3 0.11477245014131654 1108 4 0.07278384666239646 1108 5 0.0423872760407491 1108 6 0.11962540111433384 1108 7 0.020772896745093306 1108 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-1.6871641022782775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 21.0 5 76.0 6 177.0 7 400.0 8 891.0 9 1751.0 10 2842.0 11 4288.0 12 6142.0 13 8590.0 14 10435.0 15 13856.0 16 17973.0 17 24607.0 18 23748.0 19 16177.0 20 14598.0 21 15166.0 22 16467.0 23 17878.0 24 20999.0 25 25254.0 26 30199.0 27 36840.0 28 44879.0 29 54113.0 30 65974.0 31 80696.0 32 98707.0 33 124656.0 34 165996.0 35 234507.0 36 357058.0 37 564047.0 38 500768.0 39 53692.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40763353474583 19.572974297721675 14.688850362765285 22.33054180476721 2 15.533031831033139 21.206335692234088 39.86347634286877 23.39715613386401 3 19.890713264531538 25.40338333047653 29.855659384023642 24.850244020968283 4 12.973702997003187 17.14652748084845 37.023942497320576 32.855827024827796 5 13.289357164121174 38.22045362907024 33.905626462575036 14.584562744233546 6 31.05513685792808 36.33950485892265 17.723134650268705 14.882223632880562 7 27.960376494875653 33.45319336895857 20.596876606950122 17.989553529215648 8 25.95844391661659 35.22065485395472 19.693424524907847 19.12747670452084 9 26.605198371812232 17.064929732132597 20.498288745274508 35.83158315078066 10-11 24.87324283241382 26.950255887676892 27.04743104594677 21.129070233962523 12-13 26.209321205431564 24.37570752895571 27.611645950156056 21.80332531545667 14-15 23.0728559400411 25.656087715696263 26.592841926023315 24.67821441823932 16-17 21.632793759059226 28.877272988416436 27.34493479241601 22.14499846010833 18-19 21.429561666387265 27.54762223180768 29.811017956402093 21.21179814540297 20-21 22.69832001310989 26.46243998365031 29.51996338272772 21.319276620512078 22-23 22.150473632361834 26.551327281939813 29.295757934213228 22.002441151485126 24-25 22.000123188363485 26.636036589581014 29.747371557765828 21.61646866428967 26-27 22.011540250262907 26.51665684981268 29.835519506217583 21.63628339370683 28-29 21.526774496747958 26.979376413880658 29.882594928959854 21.61125416041153 30-31 21.71816755415834 26.848899127326014 29.677376214218576 21.755557104297065 32-33 21.69641192170986 26.889001068003775 29.450929276300297 21.963657733986064 34-35 22.184340394686824 26.781020432075113 29.37668852924699 21.657950643991068 36-37 22.22883274673825 26.534588889219624 29.578182807419847 21.65839555662228 38-39 22.10017712092035 26.72214395812697 29.668583059011294 21.509095861941383 40-41 22.063148170509216 26.757262269428704 29.611308205954263 21.568281354107818 42-43 21.666726633239854 26.952839712363897 29.23436374743737 22.14606990695888 44-45 21.948048498716933 27.073992245645357 28.927250229290873 22.050709026346833 46-47 22.134106670675003 27.169520533108503 28.34493452362789 22.351438272588606 48-49 22.235713260476704 27.135339810287455 28.415912677528226 22.21303425170761 50-51 22.219112181835648 27.471053707216274 28.375956164755124 21.933877946192954 52-53 22.16693633605012 27.53217667457153 28.26606667583354 22.034820313544813 54-55 21.81613382633543 27.324904830880563 28.443012486414503 22.415948856369504 56-57 22.078995257210483 27.727320588573797 27.898222249765066 22.295461904450658 58-59 22.166063377139242 27.301298241893075 28.12976730422537 22.402871076742308 60-61 22.114897944614192 27.170943218594367 28.523538035572038 22.190620801219403 62-63 22.19008762610851 27.087769263805068 28.489447947981887 22.232695162104534 64-65 21.927382772781744 27.30343398360256 28.401839334036303 22.367343909579386 66-67 21.790825928634977 27.444605334020007 28.126356858269258 22.638211879075758 68-69 21.770400043549127 27.447137822246106 28.19962226030887 22.582839873895896 70-71 21.909985057345473 27.335884616261218 27.94423317698767 22.809897149405636 72-73 21.856800848375485 27.172860272331544 27.916337820707216 23.05400105858575 74-75 21.716208506131256 27.60121734121663 27.76718245633268 22.91539169631944 76-77 21.87762453389786 27.106619592800502 28.035902492864285 22.97985338043736 78-79 21.792736182163782 27.171023952607477 28.1162034297558 22.92003643547294 80-81 21.708536239191982 27.404322584667618 27.87485258265025 23.012288593490148 82-83 21.745219455429112 27.31509683524728 27.861272569116775 23.07841114020683 84-85 21.687671996639647 27.10461727981134 27.80929257464254 23.398418148906476 86-87 21.659725232733045 27.093654579639875 27.975993267434497 23.270626920192587 88-89 21.830464974846528 26.8458277714521 27.839546350879736 23.48416090282164 90-91 21.550759147585143 26.954538139121553 28.00593905827233 23.488763655020975 92-93 21.46441524634367 27.137840003993258 27.88691538526016 23.51082936440291 94-95 21.371207446435797 26.99289653182283 27.814056096904928 23.821839924836446 96-97 21.274429196651326 26.68152832049185 27.901351770071948 24.142690712784884 98-99 21.28134082505646 26.73511057700458 27.667005087615866 24.316543510323093 100-101 21.46700152592902 26.199641846507856 28.2294608465751 24.103895780988026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1358.0 1 930.0 2 669.5 3 815.5 4 684.5 5 669.5 6 1166.0 7 2345.5 8 3663.5 9 4176.5 10 3714.5 11 3111.5 12 3051.5 13 3255.0 14 3490.0 15 3718.5 16 3950.0 17 4272.0 18 4509.5 19 4810.5 20 5378.0 21 6252.0 22 7406.5 23 8712.5 24 10306.0 25 12636.5 26 16067.0 27 20286.0 28 24080.5 29 27930.5 30 32157.5 31 36949.0 32 42383.5 33 47075.0 34 52050.0 35 57108.5 36 62960.0 37 68435.0 38 71838.0 39 74222.5 40 76440.5 41 79409.0 42 82050.5 43 85118.5 44 89118.0 45 92281.5 46 93832.5 47 99405.0 48 118890.0 49 119933.0 50 103346.0 51 97843.5 52 92963.5 53 89345.5 54 85654.5 55 78852.0 56 71052.0 57 63026.0 58 57020.5 59 51337.5 60 43651.0 61 35980.5 62 28724.0 63 21508.5 64 15273.0 65 11183.5 66 8144.0 67 6190.0 68 4831.0 69 3618.0 70 2726.5 71 2021.0 72 1519.0 73 1208.5 74 922.5 75 723.5 76 519.5 77 302.0 78 203.5 79 141.0 80 88.0 81 59.5 82 48.0 83 28.5 84 10.5 85 8.5 86 6.5 87 4.0 88 0.5 89 1.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23936846506949558 2 0.0 3 0.0 4 0.0 5 0.029195870385404322 6 0.003390488173788889 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029195870385404325 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.085732260683333E-4 26-27 0.001375031314925494 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.006705632165938026 36-37 0.017630538503702227 38-39 0.013787985240074817 40-41 0.008871777388080928 42-43 0.008608072752341791 44-45 0.003974405581496976 46-47 6.027534531180248E-4 48-49 0.0018082603593540744 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012996871332857408 58-59 3.955569536087037E-4 60-61 0.0025805382211615438 62-63 9.418022704969138E-4 64-65 0.0017329161777143213 66-67 0.005481289214292038 68-69 4.332290444285803E-4 70-71 1.3185231786956792E-4 72-73 0.0 74-75 4.332290444285803E-4 76-77 0.06151852430885841 78-79 0.1420802905271644 80-81 0.0011866708608261113 82-83 0.0010548185429565433 84-85 0.0 86-87 0.10907953896895255 88-89 0.3624620218234422 90-91 0.4005108335515175 92-93 4.709011352484569E-4 94-95 2.6370463573913584E-4 96-97 9.418022704969137E-5 98-99 0.0 100-101 0.001375031314925494 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2654485.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.71090093656478 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74708067544256 41.64227206806995 2 14.695856810020352 16.374388458419695 3 4.789578701899693 8.004952337682429 4 2.0452558545435426 4.557721852096177 5 1.0694864889949875 2.9791027920697117 6 0.6257299697420224 2.0915988214402494 7 0.4097664006277806 1.5979918746754898 8 0.2734480485057185 1.2187229713279233 9 0.18088980173767885 0.9069780442538403 >10 1.0463757954543804 10.602563539287688 >50 0.07733162632502612 2.9769517355673356 >100 0.03594733526253752 3.69977925337499 >500 0.001829526437178953 0.7460878238675093 >1k 0.0010841638146107638 1.15397483746213 >5k 2.7104095365269095E-4 1.0192079111727583 >10k+ 6.776023841317274E-5 0.4277056792320957 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11330 0.4268247889892013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9872 0.3718988805738213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6316 0.23793692561834026 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5450 0.20531289496832722 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5361 0.20196007888535816 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4833 0.18206921493246336 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2855 0.10755381929074753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.10729011465500841 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.767209081987655E-5 0.0 4 0.0 0.0 0.0 3.767209081987655E-5 0.0 5 0.0 0.0 0.0 3.767209081987655E-5 0.0 6 0.0 0.0 0.0 3.767209081987655E-5 0.0 7 0.0 0.0 0.0 3.767209081987655E-5 0.0 8 0.0 0.0 0.0 3.767209081987655E-5 0.0 9 0.0 0.0 0.0 1.8836045409938274E-4 0.0 10-11 0.0 0.0 0.0 2.6370463573913584E-4 0.0 12-13 0.0 0.0 0.0 3.3904881737888896E-4 0.0 14-15 0.0 0.0 0.0 3.3904881737888896E-4 0.0 16-17 7.53441816397531E-5 0.0 0.0 4.520650898385186E-4 0.0 18-19 7.53441816397531E-5 0.0 0.0 6.027534531180248E-4 0.0 20-21 1.506883632795062E-4 0.0 0.0 7.911139072174075E-4 0.0 22-23 1.506883632795062E-4 0.0 0.0 9.418022704969138E-4 0.0 24-25 1.506883632795062E-4 0.0 0.0 0.0015257196782050002 0.0 26-27 1.8836045409938274E-4 0.0 0.0 0.0022226533583727167 0.0 28-29 2.260325449192593E-4 0.0 0.0 0.005500125259701977 0.0 30-31 2.260325449192593E-4 0.0 0.0 0.01561508164483883 0.0 32-33 2.260325449192593E-4 0.0 0.0 0.03627822345954111 0.0 34-35 2.260325449192593E-4 0.0 0.0 0.06950500756267224 0.0 36-37 2.260325449192593E-4 0.0 0.0 0.12220826261967951 0.0 38-39 2.260325449192593E-4 0.0 0.0 0.1965541338527059 0.0 40-41 2.260325449192593E-4 0.0 0.0 0.28060056847185044 0.0 42-43 2.6370463573913584E-4 0.0 0.0 0.36297059504951057 0.0 44-45 2.6370463573913584E-4 0.0 0.0 0.46366809381104057 0.0 46-47 2.6370463573913584E-4 0.0 0.0 0.5919980711889501 0.0 48-49 2.6370463573913584E-4 0.0 0.0 0.732044068811841 0.0 50-51 2.6370463573913584E-4 0.0 0.0 0.8783247974654218 0.0 52-53 2.6370463573913584E-4 0.0 0.0 1.0228349378504682 0.0 54-55 3.013767265590124E-4 0.0 0.0 1.1850886330116763 0.0 56-57 3.013767265590124E-4 0.0 0.0 1.3828294377252086 0.0 58-59 3.013767265590124E-4 0.0 0.0 1.6020621702514801 0.0 60-61 3.3904881737888896E-4 0.0 0.0 1.8476653663516651 0.0 62-63 3.578848627888272E-4 0.0 0.0 2.0845851455178686 0.0 64-65 3.7672090819876547E-4 0.0 0.0 2.3285119335765696 0.0 66-67 3.9555695360870375E-4 0.0 0.0 2.6041962941964263 0.0 68-69 4.1439299901864204E-4 0.0 0.0 2.9215271512176564 0.0 70-71 4.520650898385186E-4 0.0 0.0 3.2715573830705393 0.0 72-73 4.897371806583951E-4 0.0 0.0 3.626240118139677 0.0 74-75 5.085732260683333E-4 0.0 0.0 3.9775135289896157 0.0 76-77 5.274092714782717E-4 0.0 0.0 4.362032559988096 0.0 78-79 5.274092714782717E-4 0.0 0.0 4.8048679875757445 0.0 80-81 5.274092714782717E-4 0.0 0.0 5.280723002767015 0.0 82-83 5.4624531688821E-4 0.0 0.0 5.756559181912875 0.0 84-85 5.650813622981482E-4 0.0 0.0 6.245712445163563 0.0 86-87 5.650813622981482E-4 0.0 0.0 6.757167586179617 0.0 88-89 5.650813622981482E-4 0.0 0.0 7.305955769198168 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2560 0.0 24.842081 2 GTACATG 14820 0.0 22.406757 1 TACATGG 14720 0.0 22.149916 2 GAGTACT 10655 0.0 21.686232 12-13 ACATGGG 14955 0.0 21.10369 3 GTACTTT 11280 0.0 20.484646 14-15 AGTACTT 11000 0.0 19.906166 12-13 GTACCTG 3305 0.0 19.57669 1 CATGGGA 9705 0.0 19.071823 4 GTATCAA 25645 0.0 18.328463 1 GTGTAGC 1690 0.0 18.016277 1 ACTTTTT 13550 0.0 17.507948 16-17 ACCTGGG 3645 0.0 17.447388 3 AGTACAT 11570 0.0 17.269186 2 CATGGGG 5400 0.0 17.050264 4 GGTATCA 20725 0.0 17.03261 1 TACTTTT 12865 0.0 16.891348 14-15 ATCAACG 28990 0.0 16.059942 3 TCAACGC 29075 0.0 16.045637 4 TATCAAC 29125 0.0 16.034384 2 >>END_MODULE