##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938739_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627087 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67990648318129 33.0 31.0 34.0 30.0 34.0 2 31.70781033835314 33.0 31.0 34.0 30.0 34.0 3 31.674567493932408 33.0 31.0 34.0 28.0 34.0 4 35.264468955870214 37.0 35.0 37.0 33.0 37.0 5 35.33766172306705 37.0 35.0 37.0 33.0 37.0 6 35.465665327053806 37.0 35.0 37.0 33.0 37.0 7 35.40915882187 37.0 35.0 37.0 33.0 37.0 8 35.40027546160715 37.0 35.0 37.0 33.0 37.0 9 36.94125145121312 39.0 37.0 39.0 33.0 39.0 10-11 36.85079224405333 39.0 37.0 39.0 33.0 39.0 12-13 36.76156161287012 39.0 37.0 39.0 32.0 39.0 14-15 37.82826333195459 40.0 37.5 41.0 32.5 41.0 16-17 37.80057796540689 40.0 37.5 41.0 32.5 41.0 18-19 37.88193440178675 40.0 38.0 41.0 33.0 41.0 20-21 37.829952854395614 40.0 37.5 41.0 32.5 41.0 22-23 37.73753462476192 40.0 37.0 41.0 32.0 41.0 24-25 37.71733994555915 40.0 37.0 41.0 32.0 41.0 26-27 37.6055997620287 40.0 37.0 41.0 32.0 41.0 28-29 37.49210982571921 40.0 37.0 41.0 32.0 41.0 30-31 37.22523319281636 39.5 36.5 41.0 32.0 41.0 32-33 36.90711805822307 39.0 35.0 41.0 31.0 41.0 34-35 36.545240666295044 39.0 35.0 41.0 30.5 41.0 36-37 36.24908840154214 39.0 35.0 41.0 30.0 41.0 38-39 36.04591364813314 39.0 35.0 41.0 30.0 41.0 40-41 35.86317111500492 39.0 35.0 41.0 29.5 41.0 42-43 35.51965014777944 39.0 35.0 41.0 27.5 41.0 44-45 35.180394471838326 39.0 35.0 41.0 24.5 41.0 46-47 34.70274453670885 39.0 35.0 41.0 21.5 41.0 48-49 34.283084432485786 39.0 35.0 41.0 16.0 41.0 50-51 33.1201407791962 38.0 33.0 40.0 9.0 40.5 52-53 33.24099233784057 38.0 33.5 39.5 8.5 40.5 54-55 33.78915632661314 39.0 34.0 40.5 7.5 41.0 56-57 33.73081586909612 39.0 34.0 41.0 2.0 41.0 58-59 33.56600907019723 39.0 34.0 41.0 2.0 41.0 60-61 33.29770350325459 38.0 34.0 40.0 2.0 41.0 62-63 32.988031678699414 38.0 33.0 40.0 2.0 41.0 64-65 32.646403664954605 38.0 33.0 40.0 2.0 41.0 66-67 32.255614174288155 37.0 32.0 40.0 2.0 41.0 68-69 31.860582746958215 37.0 32.0 39.0 2.0 41.0 70-71 31.44498911244451 36.0 31.0 39.0 2.0 41.0 72-73 31.001517435760967 36.0 31.0 39.0 2.0 40.0 74-75 30.522398925195766 35.0 31.0 38.0 2.0 40.0 76-77 30.058405604617334 35.0 30.0 37.0 2.0 39.5 78-79 29.581387780739444 35.0 29.5 37.0 2.0 39.0 80-81 29.082675665161112 35.0 29.0 36.0 2.0 39.0 82-83 28.553478701507665 34.0 29.0 36.0 2.0 37.5 84-85 28.05991320685372 34.0 27.5 35.0 2.0 37.0 86-87 27.658459566083437 34.0 27.0 35.0 2.0 37.0 88-89 27.20836839087277 34.0 26.0 35.0 2.0 36.0 90-91 26.727255825902365 34.0 25.0 35.0 2.0 36.0 92-93 26.24862499669655 34.0 24.5 35.0 2.0 35.5 94-95 25.736983640088084 34.0 20.0 35.0 2.0 35.0 96-97 25.157141259195114 33.0 12.0 35.0 2.0 35.0 98-99 24.539035097692995 33.0 2.0 35.0 2.0 35.0 100-101 22.488722483800807 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1704040619312579 1101 2 0.08731464580376525 1101 3 0.12295386039873435 1101 4 0.16448564963235413 1101 5 0.008367139446050942 1101 6 -0.1310054973064041 1101 7 -0.04560209229861556 1101 8 0.14924490106402288 1101 9 0.12468814556672925 1101 10-11 0.2118912184185504 1101 12-13 0.22779715180637794 1101 14-15 0.30701777812063114 1101 16-17 0.3736421024647072 1101 18-19 0.3574970295177522 1101 20-21 0.4448723040693281 1101 22-23 0.45727510238128133 1101 24-25 0.3710822181773139 1101 26-27 0.4131033118305041 1101 28-29 0.2753076853901817 1101 30-31 0.323881213639595 1101 32-33 0.2936455836995506 1101 34-35 0.3552861585143603 1101 36-37 0.4715457608119564 1101 38-39 0.553190505444924 1101 40-41 0.46123319225397097 1101 42-43 0.4331387558748929 1101 44-45 0.5368059597440862 1101 46-47 0.4730891262698762 1101 48-49 0.32643562644985735 1101 50-51 0.1620828962463392 1101 52-53 0.23028134134030864 1101 54-55 0.20922130955040785 1101 56-57 0.09593511869211824 1101 58-59 0.16733353448505284 1101 60-61 0.1350481446732985 1101 62-63 0.11405969872934207 1101 64-65 0.10412484590056437 1101 66-67 0.22343868362320762 1101 68-69 0.17098700030497582 1101 70-71 0.13681238021833764 1101 72-73 0.02698988951217629 1101 74-75 -0.0035398092553045046 1101 76-77 -0.005007489547548261 1101 78-79 0.01505309185091619 1101 80-81 0.16596390439480402 1101 82-83 0.045457824776921285 1101 84-85 -0.01897550914984336 1101 86-87 0.06821071370440634 1101 88-89 -0.10277089381752091 1101 90-91 -0.071602157395386 1101 92-93 -0.09805345942443466 1101 94-95 -0.2297009289637053 1101 96-97 -0.1624393708615024 1101 98-99 -0.26424539150090354 1101 100-101 -0.1314077728802978 1102 1 0.34995200979724217 1102 2 0.27909823608034046 1102 3 0.3542998860458191 1102 4 0.32972718677757484 1102 5 0.35276819136485926 1102 6 0.330691850325465 1102 7 0.4165440530278488 1102 8 0.3832705901390838 1102 9 0.36254567460734677 1102 10-11 0.33993110104876934 1102 12-13 0.36693887214504883 1102 14-15 0.11032759550458593 1102 16-17 0.4358937546176307 1102 18-19 0.5607682660472619 1102 20-21 0.5471588102186544 1102 22-23 0.48706634583959385 1102 24-25 0.3417581442490487 1102 26-27 0.44361105687142555 1102 28-29 0.6733964895936637 1102 30-31 0.7318475039460921 1102 32-33 0.48013021172236847 1102 34-35 0.48190878526689573 1102 36-37 0.342561040929958 1102 38-39 0.1662625912781479 1102 40-41 0.38969326500643575 1102 42-43 0.4568384236071239 1102 44-45 0.41711738860046665 1102 46-47 0.31171092366526665 1102 48-49 0.11251003763252498 1102 50-51 0.09757282060686379 1102 52-53 0.03619602253647258 1102 54-55 -0.025830577762000928 1102 56-57 0.07791103584039405 1102 58-59 -1.2968247582989534E-4 1102 60-61 -0.0876683674074954 1102 62-63 -0.058224812763562284 1102 64-65 0.061969357183492946 1102 66-67 0.005572336101565156 1102 68-69 -0.006529547469668273 1102 70-71 -0.043053137946159836 1102 72-73 -0.03617641759142387 1102 74-75 0.029117440533415362 1102 76-77 -0.06029621606440472 1102 78-79 -0.18556210218191183 1102 80-81 -0.25154261760678054 1102 82-83 -0.34321016466189747 1102 84-85 -0.5237062567785635 1102 86-87 -0.45346542758025166 1102 88-89 -0.3361850632268357 1102 90-91 -0.3643665412302717 1102 92-93 -0.4009945278254037 1102 94-95 -0.4435766513705204 1102 96-97 -0.4427843939312801 1102 98-99 -0.4026679857090478 1102 100-101 -0.28716944293263325 1103 1 0.1781692854701582 1103 2 0.2255279680433162 1103 3 0.11078642933290794 1103 4 0.25822982730985444 1103 5 0.16836307285952756 1103 6 0.19571410304511971 1103 7 0.1969017339509449 1103 8 0.1960527582536713 1103 9 0.3154165835125724 1103 10-11 0.3020871292020715 1103 12-13 0.402174806679092 1103 14-15 0.3438444198773283 1103 16-17 0.45373981964637977 1103 18-19 0.5210201737548132 1103 20-21 0.4653132819898147 1103 22-23 0.5798071820489454 1103 24-25 0.41500347953377315 1103 26-27 0.25829291430040513 1103 28-29 0.24778442374958587 1103 30-31 0.19541685199584435 1103 32-33 0.17681884836031259 1103 34-35 0.1161885238554703 1103 36-37 0.07108482428899521 1103 38-39 0.04485655531761523 1103 40-41 0.26964294423472523 1103 42-43 0.14030751836497046 1103 44-45 0.06300183819909222 1103 46-47 0.1405316772276919 1103 48-49 0.22537074227525267 1103 50-51 0.16702384141292725 1103 52-53 0.2937992109253855 1103 54-55 0.2654629243425788 1103 56-57 0.30940241117071565 1103 58-59 0.23716035575006345 1103 60-61 0.2216058217165724 1103 62-63 0.10906643241811764 1103 64-65 -0.058781504355046366 1103 66-67 0.08583130829854468 1103 68-69 0.03758658530896852 1103 70-71 0.016485020002992457 1103 72-73 0.13726882501934767 1103 74-75 0.12968428773703877 1103 76-77 0.1927260265269517 1103 78-79 0.07583877678574424 1103 80-81 0.04290606102984995 1103 82-83 0.11884078237637752 1103 84-85 0.20959217612017866 1103 86-87 0.3008211644914809 1103 88-89 0.25235674850662804 1103 90-91 0.2026665588989509 1103 92-93 0.4172385648763992 1103 94-95 0.36778016949107695 1103 96-97 0.46191969511673747 1103 98-99 0.7324232829365194 1103 100-101 0.7846961224501889 1104 1 0.11933940764313533 1104 2 0.18768240553407622 1104 3 0.10400275234947287 1104 4 0.22308152677223347 1104 5 0.23313156975195426 1104 6 0.15498679122119796 1104 7 0.17759328048024514 1104 8 0.1424540943818755 1104 9 0.2059308796526338 1104 10-11 0.14269882081254082 1104 12-13 0.19882108742647375 1104 14-15 0.23226297238068128 1104 16-17 0.2757594225465354 1104 18-19 0.16094608559266987 1104 20-21 0.33786612661766213 1104 22-23 0.25097865224201854 1104 24-25 0.2773423229162333 1104 26-27 0.10105736550350031 1104 28-29 0.24087347900741207 1104 30-31 0.2325016587504436 1104 32-33 0.22258209449045552 1104 34-35 0.20460703563313132 1104 36-37 0.3444521011214121 1104 38-39 0.20235397025489732 1104 40-41 0.04663053063935507 1104 42-43 0.2442427189165599 1104 44-45 0.3993008836298131 1104 46-47 0.3976954266458179 1104 48-49 0.5476680943320886 1104 50-51 0.5071474357150763 1104 52-53 0.4127542469207839 1104 54-55 0.5210359285906705 1104 56-57 0.4769661967532599 1104 58-59 0.3254966016484815 1104 60-61 0.4599260013331232 1104 62-63 0.40501813664540265 1104 64-65 0.4237123572664174 1104 66-67 0.4233584366939951 1104 68-69 0.3208864489186567 1104 70-71 0.43505340636591683 1104 72-73 0.36567963149782656 1104 74-75 0.3299226958841288 1104 76-77 0.4916445967459744 1104 78-79 0.48539803122881864 1104 80-81 0.5258696934023668 1104 82-83 0.4374176396813212 1104 84-85 0.4257879249290575 1104 86-87 0.4230743699081607 1104 88-89 0.474807962193065 1104 90-91 0.4021531709416166 1104 92-93 0.35077445418073694 1104 94-95 0.38377945760614196 1104 96-97 0.5458001440603759 1104 98-99 0.2574801665339628 1104 100-101 0.683336006821829 1105 1 0.07362737010074838 1105 2 0.08614786614102954 1105 3 0.048041273652032146 1105 4 0.0363100156482119 1105 5 0.06697401214645282 1105 6 0.031675835996452406 1105 7 0.18102232145491115 1105 8 0.16235371501154816 1105 9 0.19486378462934084 1105 10-11 0.14021425514354746 1105 12-13 0.06974421378155427 1105 14-15 0.2035140111617011 1105 16-17 0.010904398087824063 1105 18-19 0.08628614329844453 1105 20-21 0.040743757706664496 1105 22-23 0.12197957321686914 1105 24-25 0.11904384521918132 1105 26-27 0.23871291928976746 1105 28-29 0.1768811239495207 1105 30-31 0.06362777233788108 1105 32-33 0.056035158605780566 1105 34-35 0.15053410236673415 1105 36-37 0.03093471868346853 1105 38-39 0.1008342199432164 1105 40-41 0.1364164617254815 1105 42-43 0.121395289967964 1105 44-45 0.034426694142375425 1105 46-47 0.15657951144682158 1105 48-49 0.1395299667432326 1105 50-51 0.39265138075317907 1105 52-53 0.30627691290999337 1105 54-55 0.37668720124177213 1105 56-57 0.3397095429834778 1105 58-59 0.3270065060785967 1105 60-61 0.5292869955159532 1105 62-63 0.3870702695753394 1105 64-65 0.5034504244023594 1105 66-67 0.3131226501154245 1105 68-69 0.325723694065303 1105 70-71 0.1997899155835654 1105 72-73 0.2884437553744128 1105 74-75 0.2619235127276376 1105 76-77 0.30848931990044903 1105 78-79 0.21052613911374607 1105 80-81 0.22208676794379656 1105 82-83 0.12792146924303083 1105 84-85 0.26985387693868645 1105 86-87 0.3190086691103211 1105 88-89 0.32890901497306757 1105 90-91 0.2038530644197145 1105 92-93 0.12849922086778065 1105 94-95 0.22975887363596215 1105 96-97 0.06026001642047518 1105 98-99 0.12021358584656028 1105 100-101 0.22289902795968075 1106 1 0.1836801034936535 1106 2 0.25015824627624994 1106 3 0.3080334749388989 1106 4 0.2210158103963309 1106 5 0.26584560080997477 1106 6 0.24921149068801185 1106 7 0.2527780888194613 1106 8 0.23381282701515715 1106 9 0.153154681433314 1106 10-11 0.3261264883939319 1106 12-13 0.3116368349498515 1106 14-15 0.4163922567555005 1106 16-17 0.26941412325925995 1106 18-19 0.3115722695959633 1106 20-21 0.2748188495457171 1106 22-23 0.4306412692047772 1106 24-25 0.37149055134381115 1106 26-27 0.20648297575437624 1106 28-29 0.2903672127747825 1106 30-31 0.20098460910296723 1106 32-33 0.2613589012834794 1106 34-35 0.2806070691765612 1106 36-37 0.35336646793322046 1106 38-39 0.26593834157110763 1106 40-41 0.32087544401743884 1106 42-43 0.14973968882650013 1106 44-45 0.21938010203589187 1106 46-47 0.11737296244944417 1106 48-49 0.3926752742239117 1106 50-51 0.2637023814545856 1106 52-53 0.2246568543635874 1106 54-55 0.246095587981074 1106 56-57 0.2515042158378762 1106 58-59 0.19272151355703215 1106 60-61 0.16136749040595078 1106 62-63 0.11026999990320263 1106 64-65 0.1621590407793363 1106 66-67 0.041800496005173216 1106 68-69 0.36725844718078804 1106 70-71 0.4800345501650156 1106 72-73 0.4874570660322277 1106 74-75 0.25323042972178555 1106 76-77 0.19077178864954547 1106 78-79 0.4358072611035553 1106 80-81 0.4221593904572529 1106 82-83 0.393823157416211 1106 84-85 0.44613181698948523 1106 86-87 0.6417148545947846 1106 88-89 0.795064481598736 1106 90-91 0.8166513611935748 1106 92-93 1.0363502990759024 1106 94-95 1.0822731178974259 1106 96-97 1.1116664670932117 1106 98-99 1.0993542301131463 1106 100-101 1.2586480006753966 1107 1 0.05628838223903543 1107 2 -0.04894854627488954 1107 3 0.02007078516272287 1107 4 0.040265478474381666 1107 5 -0.01801867685568226 1107 6 -0.002212321062806666 1107 7 -0.04642992815485769 1107 8 0.11212459196106295 1107 9 -0.06480621926003494 1107 10-11 -0.019207497274571494 1107 12-13 -0.02032238337788783 1107 14-15 0.07938966886288767 1107 16-17 0.049589986962530475 1107 18-19 0.11003424687775265 1107 20-21 0.03917466987158491 1107 22-23 0.054412059445802186 1107 24-25 -0.006172867248217528 1107 26-27 0.08021355086603421 1107 28-29 -0.017197704252573942 1107 30-31 0.09211558409033671 1107 32-33 0.10714486577175109 1107 34-35 0.01871261093909027 1107 36-37 0.25504636178097684 1107 38-39 0.171118128701643 1107 40-41 0.05779924949077753 1107 42-43 0.18505053412105354 1107 44-45 0.05789752519164182 1107 46-47 0.3250383976830804 1107 48-49 0.20668187028982032 1107 50-51 0.3940994479864912 1107 52-53 0.2709307362652993 1107 54-55 0.12121211377054664 1107 56-57 0.0853296123394287 1107 58-59 0.1805351523317995 1107 60-61 -0.0018427676300163398 1107 62-63 -0.05555561474091064 1107 64-65 0.09812635392218994 1107 66-67 0.03311461657679615 1107 68-69 -0.016482360229154835 1107 70-71 0.02899401802272905 1107 72-73 -0.13695520456498045 1107 74-75 -0.1857955873090269 1107 76-77 -0.1444613335510887 1107 78-79 -0.10928154959578862 1107 80-81 -0.1949623676148029 1107 82-83 -0.08369638909837107 1107 84-85 -0.1472141680889365 1107 86-87 -0.1259864721513786 1107 88-89 -0.1036379233052287 1107 90-91 0.09480155940512347 1107 92-93 -0.04819563484102929 1107 94-95 -0.01309245136048176 1107 96-97 -0.00530090411636408 1107 98-99 0.02143947677116742 1107 100-101 -0.06464691707387615 1108 1 0.07999731338498606 1108 2 0.149719750523861 1108 3 0.12453452559055123 1108 4 0.10974722224246847 1108 5 0.0765928981366315 1108 6 0.08949032463539197 1108 7 0.1594512787344442 1108 8 0.16619788386208967 1108 9 0.134089699326978 1108 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2316 86-87 -2.011581575472217 2316 88-89 -1.8642987407630685 2316 90-91 -2.1974425458416 2316 92-93 -2.3844215288135473 2316 94-95 -2.408417313425854 2316 96-97 -2.6070743725215273 2316 98-99 -2.8702764754982475 2316 100-101 -2.921196534500485 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6314.0 3 1433.0 4 1291.0 5 1446.0 6 1629.0 7 2016.0 8 2559.0 9 3242.0 10 4307.0 11 6338.0 12 9494.0 13 13192.0 14 14974.0 15 17123.0 16 23271.0 17 31815.0 18 24579.0 19 13267.0 20 11928.0 21 11720.0 22 12520.0 23 13629.0 24 15416.0 25 17213.0 26 20167.0 27 23125.0 28 27025.0 29 31866.0 30 37395.0 31 44737.0 32 54253.0 33 67220.0 34 88075.0 35 126844.0 36 166858.0 37 270721.0 38 332431.0 39 75622.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.523003160664445 22.54943848032003 16.084851589135784 18.84270676987974 2 13.964057076555278 24.127111855923165 41.66977346803932 20.23905759948224 3 18.056482564273228 30.75241803175322 32.765829703389336 18.425269700584217 4 10.404653440735572 19.912114253930984 42.519313452598055 27.163918852735385 5 9.735253392412075 41.34188744269054 36.12468075940799 12.798178405489399 6 23.87262343270909 41.83182668051275 19.612125281210094 14.683424605568069 7 22.597006372403143 37.62164993839108 24.41797406276977 15.363369626436004 8 23.623452269615143 34.44927945260903 22.334540343213842 19.59272793456199 9 25.72970360527599 18.501504324818562 24.34479014161498 31.42400192829047 10-11 21.862563258770916 29.137141295368696 29.387947469049664 19.612347976810728 12-13 24.9712456645595 28.376293945094822 29.23964651767396 17.412813872671716 14-15 21.833580365792507 28.918723400199653 30.320767113415727 18.926929120592114 16-17 17.131314994581384 32.167954068574325 31.274105159599408 19.426625777244883 18-19 16.927766296103396 30.640462213061483 35.15633695164927 17.275434539185852 20-21 18.912537837035945 28.679281830382468 34.64226033725501 17.76591999532658 22-23 18.30820512978207 28.758360265560473 34.73700718636896 18.196427418288494 24-25 18.39883251728465 29.543986664370248 34.77852889796521 17.278651920379893 26-27 17.999526971686887 28.72610522696286 36.03989800057883 17.234469800771425 28-29 16.889368907466373 29.84907092417996 35.82186814018155 17.439692028172114 30-31 17.722701463100844 29.440576556808544 35.19196933459691 17.644752645493696 32-33 17.489112137679765 29.72905249079547 35.27203129924135 17.509804072283412 34-35 18.15082659275612 29.309844212062945 35.02272613786348 17.51660305731746 36-37 18.204668351403495 28.882274691152166 35.24014910662013 17.672907850824213 38-39 17.52920536043124 30.10900473787338 34.965919232950434 17.395870668744948 40-41 17.42235061550071 29.94662111174382 35.23907846958916 17.391949803166316 42-43 17.159518886742525 30.041978085691856 34.09946864394143 18.699034383624195 44-45 17.714165385564232 29.600830197604193 33.94985776906934 18.735146647762235 46-47 18.632810087811386 30.25336857315763 33.09189319369141 18.021928145339576 48-49 18.261792243798965 30.264754375026442 32.8924105886927 18.581042792481895 50-51 17.537419200649175 30.571627424134913 33.25805338021811 18.632899994997803 52-53 17.800477217304948 31.420377679174965 32.321513656462216 18.457631447057878 54-55 17.62461666149991 30.479100564061838 32.528656984449086 19.36762578998917 56-57 18.349326224101333 30.540391361739804 31.848583483877658 19.261698930281206 58-59 18.637878891224567 29.941584757655598 32.33411901376479 19.086417337355048 60-61 18.193896142163148 30.584175263377933 32.745872113024575 18.476056481434345 62-63 17.671827485605792 30.386335921460876 32.98726517759289 18.954571415340443 64-65 17.639023450155335 30.819926192652066 32.47634492720412 19.06470542998849 66-67 17.934042040632278 30.255017264702794 31.609941256771528 20.200999437893397 68-69 17.62501918928995 30.204019604118386 32.19417677251124 19.97678443408043 70-71 18.510818365739524 30.18921715494741 31.803555157449303 19.496409321863755 72-73 18.339638841104293 29.451590686584865 32.37925081302445 19.82951965928639 74-75 17.85726638469444 31.149695628112894 31.309324106648745 19.683713880543916 76-77 18.067273075331766 30.08639162143303 31.783625730994153 20.062709572241054 78-79 17.978564763325565 29.929568878315628 32.028426799571186 20.063439558787625 80-81 18.529167528454433 30.325647729973852 31.13802289937303 20.007161842198688 82-83 18.42820068082447 29.959703786399196 31.623327345914525 19.988768186861805 84-85 18.234024154292573 29.917973951349964 31.302713444782228 20.545288449575235 86-87 18.218106210636627 30.209581892183795 31.39242061867384 20.17989127850574 88-89 18.658392466014213 29.532979715835804 31.134140063779608 20.674487754370375 90-91 18.88970620932956 29.06550010129009 31.430345769779976 20.614447919600373 92-93 19.536069309502167 29.75572501639072 31.031159196729703 19.67704647737741 94-95 19.16076845298281 29.143269921488233 31.139665211646992 20.556296413881963 96-97 19.01139110873376 28.95458380636721 31.242132401719104 20.791892683179924 98-99 19.482792697087234 29.131883538799812 30.297144057118857 21.088179706994094 100-101 19.76094072427964 28.4128597105364 30.904699576454224 20.921499988729735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9478.0 1 5501.0 2 1718.0 3 1819.5 4 1426.0 5 1495.5 6 2772.5 7 4962.0 8 6894.0 9 7297.0 10 6233.5 11 5344.0 12 5397.5 13 5596.0 14 5741.0 15 5761.0 16 5952.0 17 6406.5 18 6751.5 19 7286.5 20 7999.0 21 8808.0 22 9786.5 23 10841.0 24 12049.5 25 14156.5 26 17820.0 27 21544.0 28 23822.0 29 27092.5 30 32156.5 31 37129.0 32 44553.5 33 54083.5 34 62664.5 35 68572.5 36 75986.5 37 83037.5 38 80444.5 39 73634.5 40 68016.0 41 62652.0 42 57017.5 43 54746.0 44 53331.0 45 49963.5 46 48065.5 47 52481.0 48 72511.0 49 70271.5 50 44458.5 51 29754.0 52 21403.5 53 16839.5 54 13365.5 55 10744.0 56 8376.0 57 6365.0 58 4958.0 59 3897.5 60 2899.0 61 2159.0 62 1692.5 63 1308.0 64 1022.5 65 801.5 66 595.5 67 481.5 68 354.5 69 228.5 70 158.0 71 135.5 72 116.0 73 102.5 74 90.5 75 82.0 76 78.0 77 61.0 78 61.5 79 53.5 80 35.0 81 25.0 82 16.0 83 12.0 84 6.5 85 4.5 86 3.5 87 3.5 88 1.5 89 1.0 90 0.5 91 0.5 92 2.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16870640598812478 2 0.09962589584945367 3 0.09145177854656819 4 0.1103198538246572 5 0.13133901260350553 6 0.03896534112804048 7 0.04296021048659353 8 0.04634048455921533 9 0.047692594188264055 10-11 0.04495764516587005 12-13 0.04981294792472683 14-15 0.04809208112411936 16-17 0.046063916680546275 18-19 0.050642651560734 20-21 0.05343906011172113 22-23 0.06711380522369116 24-25 0.08401517558680022 26-27 0.08552093403733174 28-29 0.07522646299798351 30-31 0.06268871916498625 32-33 0.05220986953985865 34-35 0.30397882842159024 36-37 0.48202708275586986 38-39 0.45962508458367624 40-41 0.434180839746123 42-43 0.4641116301709743 44-45 0.4740680738030603 46-47 0.47366858686720503 48-49 0.4906006869946107 50-51 0.4792614039691793 52-53 0.4860526818787195 54-55 0.47698740141123364 56-57 0.4553536473464541 58-59 0.43313602776003984 60-61 0.40283648016362983 62-63 0.4616839787915459 64-65 0.5004342115695104 66-67 0.5025545653059732 68-69 0.5129104958739145 70-71 0.5108516016660448 72-73 0.4997888865192826 74-75 0.4925981216738871 76-77 0.47428318215313625 78-79 0.4472717193364583 80-81 0.45430883536037103 82-83 0.46586322673587827 84-85 0.46343557535644986 86-87 0.47446756073891566 88-89 0.4866058176360576 90-91 0.49013974053016224 92-93 0.4935507443670805 94-95 0.49745342443274393 96-97 0.4981294792472683 98-99 0.4921064454451421 100-101 0.47824732174739276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627087.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.917278499830132 #Duplication Level Percentage of deduplicated Percentage of total 1 72.52487253039767 22.422716821869844 2 11.927285946722508 7.375184427238597 3 4.966819639511252 4.606816381595858 4 2.7181800198040054 3.3615491473981685 5 1.712290486465546 2.6469680921332452 6 1.0780384934622804 1.9998009801546373 7 0.8053001809724479 1.7428382979762143 8 0.5911529378062146 1.4621472011318 9 0.45644215464012844 1.2700754292664242 >10 2.609057437011362 15.164722161215593 >50 0.30252423706319764 6.640186260616004 >100 0.2758739520056731 16.687417333081413 >500 0.021174970922929998 4.336834565956979 >1k 0.009388902201676508 5.273263248920845 >5k 9.988193831570753E-4 2.288389101779262 >10k+ 5.992916298942452E-4 2.721090549665085 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17852 1.0971755044444458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13584 0.8348662364089935 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12622 0.7757421699024084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9366 0.5756299448031974 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8708 0.535189574988922 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7842 0.4819656232272767 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5678 0.34896720335175685 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5458 0.33544610706126965 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4474 0.2749699309256358 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3591 0.2207011671779075 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3520 0.2163375406477957 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3214 0.19753092489829985 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3170 0.1948267056402024 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3094 0.19015578146712497 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3077 0.1891109694810419 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2838 0.1744221421472853 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2791 0.17153354430340848 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2751 0.16907516315968352 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2710 0.16655532248736546 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2625 0.16133126255694993 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2213 0.13600993677658293 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2064 0.1268524670162075 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1993 0.1224888404860957 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1827 0.11228655873963715 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.11105736816777467 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1752 0.10767709409515286 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1705 0.10478849625127605 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.145952859312379E-5 0.0 10-11 0.0 0.0 0.0 9.218929288968568E-5 0.0 12-13 0.0 0.0 0.0 1.2291905718624758E-4 0.0 14-15 0.0 0.0 0.0 1.8437858577937137E-4 0.0 16-17 0.0 0.0 0.0 3.994869358553046E-4 0.0 18-19 0.0 0.0 0.0 6.760548145243616E-4 0.0 20-21 0.0 0.0 0.0 0.0015057584505315327 0.0 22-23 0.0 0.0 0.0 0.00341100383691837 0.0 24-25 0.0 0.0 0.0 0.007252224373988607 0.0 26-27 0.0 0.0 0.0 0.012752852183073185 0.0 28-29 0.0 0.0 0.0 0.030545385710782517 0.0 30-31 0.0 0.0 0.0 0.07593324757680443 0.0 32-33 0.0 0.0 0.0 0.15798171824862467 0.0 34-35 0.0 0.0 0.0 0.27954866580582355 0.0 36-37 0.0 0.0 0.0 0.4502832362375214 0.0 38-39 0.0 0.0 0.0 0.7002391390257559 0.0 40-41 0.0 0.0 0.0 0.9832602681970909 0.0 42-43 0.0 0.0 0.0 1.2510394342773312 0.0 44-45 0.0 0.0 0.0 1.5598735654577782 0.0 46-47 0.0 0.0 0.0 1.9272478976231757 0.0 48-49 0.0 0.0 0.0 2.29929315396165 0.0 50-51 0.0 0.0 0.0 2.7016994174251288 0.0 52-53 0.0 0.0 0.0 3.080290113558771 0.0 54-55 0.0 0.0 0.0 3.483956297358408 0.0 56-57 0.0 0.0 0.0 3.9628489441560286 0.0 58-59 0.0 0.0 0.0 4.460640395996034 0.0 60-61 0.0 0.0 0.0 5.019952835957758 0.0 62-63 0.0 0.0 0.0 5.571459915788154 0.0 64-65 0.0 0.0 0.0 6.113532957979506 0.0 66-67 0.0 0.0 0.0 6.715590500077747 0.0 68-69 0.0 0.0 0.0 7.376925757504055 0.0 70-71 0.0 0.0 0.0 8.110537420555875 0.0 72-73 0.0 0.0 0.0 8.835698398426144 0.0 74-75 0.0 0.0 0.0 9.585504647262255 0.0 76-77 0.0 0.0 0.0 10.328273780074452 0.0 78-79 0.0 0.0 0.0 11.19331664502267 0.0 80-81 0.0 0.0 0.0 12.120372174321348 0.0 82-83 0.0 0.0 0.0 13.025117894740724 0.0 84-85 0.0 0.0 0.0 13.94209406135013 0.0 86-87 0.0 0.0 0.0 14.873759055293295 0.0 88-89 0.0 0.0 0.0 15.851641614738487 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGCGG 25 0.0016062685 38.04795 92-93 CATTCCG 285 0.0 36.550365 9 CTTTGCG 255 0.0 31.595373 2 AGTCGGT 85 6.528478E-4 27.852552 7 ATGGGCG 110 9.625347E-5 25.85076 5 GATGACC 265 1.8189894E-12 25.014748 9 CATGGGG 4365 0.0 24.755136 4 GAGTACT 13145 0.0 24.404467 12-13 GTACAGG 450 0.0 24.234306 1 GTACATG 12670 0.0 24.212687 1 TACAGGG 435 0.0 23.968904 2 GTACTTT 14020 0.0 22.848291 14-15 TACATGG 13100 0.0 22.755903 2 AGTCCGT 105 0.0022379383 22.54661 8 ATTGCCG 105 0.0022379383 22.54661 8 GATTGGC 615 0.0 22.327332 9 ACATGGG 12340 0.0 22.313913 3 TAGGCAT 470 0.0 22.183985 5 AGTACTT 13870 0.0 22.121733 12-13 TTAGGCA 630 0.0 21.815853 4 >>END_MODULE