##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938739_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627087 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.928160571622783 31.0 31.0 34.0 30.0 34.0 2 32.138603528883216 33.0 31.0 34.0 30.0 34.0 3 32.18964751116566 34.0 31.0 34.0 30.0 34.0 4 35.859955859766565 37.0 35.0 37.0 35.0 37.0 5 35.79006654223161 37.0 35.0 37.0 35.0 37.0 6 35.83960968282581 37.0 35.0 37.0 35.0 37.0 7 35.78572688491765 37.0 35.0 37.0 33.0 37.0 8 35.768386693520384 37.0 35.0 37.0 33.0 37.0 9 37.37343608547054 39.0 37.0 39.0 34.0 39.0 10-11 37.308112596314764 39.0 37.0 39.0 34.0 39.0 12-13 37.26371730583552 39.0 37.0 39.0 33.0 39.0 14-15 38.366903552176375 40.0 38.0 41.0 33.0 41.0 16-17 38.327994139219356 40.0 38.0 41.0 33.0 41.0 18-19 38.44830147373804 40.0 38.0 41.0 33.5 41.0 20-21 38.54368020886406 40.0 38.0 41.0 34.0 41.0 22-23 38.480458942883814 40.0 38.0 41.0 34.0 41.0 24-25 38.39175440526536 40.0 38.0 41.0 34.0 41.0 26-27 38.19559218406883 40.0 37.5 41.0 33.5 41.0 28-29 38.02862354625167 40.0 37.0 41.0 33.0 41.0 30-31 37.73041699675555 40.0 36.5 41.0 33.0 41.0 32-33 37.3966739332316 39.5 35.5 41.0 32.5 41.0 34-35 37.23342666987076 39.5 35.0 41.0 32.0 41.0 36-37 37.06780307383686 40.0 35.0 41.0 31.5 41.0 38-39 36.90847969407905 40.0 35.0 41.0 31.0 41.0 40-41 36.71951131070435 40.0 35.0 41.0 31.0 41.0 42-43 36.44020725382232 39.5 35.0 41.0 30.0 41.0 44-45 36.01364032777596 39.0 35.0 41.0 29.5 41.0 46-47 35.46320018536194 39.0 35.0 41.0 26.0 41.0 48-49 34.9798916714349 39.0 35.0 41.0 23.5 41.0 50-51 34.69182164198964 39.0 35.0 41.0 22.5 41.0 52-53 34.522181051166896 39.0 35.0 41.0 21.5 41.0 54-55 34.32732545954826 39.0 34.5 40.5 19.5 41.0 56-57 34.08564200930866 38.5 34.0 40.0 17.5 41.0 58-59 33.83054870452533 38.0 34.0 40.0 13.5 41.0 60-61 33.48744812047543 38.0 33.5 40.0 8.5 41.0 62-63 33.160611571477126 38.0 33.0 40.0 7.5 41.0 64-65 32.82733467847755 37.5 33.0 40.0 3.5 41.0 66-67 32.43196307265684 37.0 32.0 39.5 2.0 41.0 68-69 31.97784844940682 36.0 32.0 39.0 2.0 40.5 70-71 31.49520738596031 36.0 31.0 39.0 2.0 40.0 72-73 31.14247824486337 35.0 31.0 38.0 2.0 40.0 74-75 30.713106305932012 35.0 31.0 37.5 2.0 39.5 76-77 28.647618719834895 33.0 28.0 35.5 2.0 38.5 78-79 29.639013156641287 34.0 29.5 36.0 2.0 39.0 80-81 29.620053813963235 34.5 30.0 36.0 2.0 38.5 82-83 29.264345729515384 34.0 29.5 36.0 2.0 37.5 84-85 28.829290627975027 34.0 29.0 35.0 2.0 37.0 86-87 28.31831579995415 34.0 29.0 35.0 2.0 36.5 88-89 27.779733044391605 34.0 27.5 35.0 2.0 36.0 90-91 27.337866075999624 34.0 26.0 35.0 2.0 36.0 92-93 27.05177381418449 34.0 26.0 35.0 2.0 35.0 94-95 26.674982038452768 33.0 25.5 35.0 2.0 35.0 96-97 26.17753936943753 33.0 24.5 35.0 2.0 35.0 98-99 25.527719169288428 33.0 21.5 35.0 2.0 35.0 100-101 22.625303686895663 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09780464169084624 1101 2 0.10500000542219112 1101 3 0.19286677116775763 1101 4 0.0993909054220623 1101 5 0.14756491234285818 1101 6 0.12189421214227991 1101 7 0.21322814503358956 1101 8 0.10423268773956096 1101 9 0.12182575378793103 1101 10-11 0.08777314043680207 1101 12-13 0.12359521422335007 1101 14-15 0.20040423925583895 1101 16-17 0.12415359570194084 1101 18-19 0.08177593956236962 1101 20-21 0.19796769431855665 1101 22-23 0.22146464801800647 1101 24-25 -0.007800221894235904 1101 26-27 0.19657448064442207 1101 28-29 0.20435284783130925 1101 30-31 0.15853611142458846 1101 32-33 0.20979707433561856 1101 34-35 0.1502665547500257 1101 36-37 0.26892415960164584 1101 38-39 0.1112021864586552 1101 40-41 0.1626688282842892 1101 42-43 0.22295840058340133 1101 44-45 0.23852684911792466 1101 46-47 0.33976318530988436 1101 48-49 0.2690596477292999 1101 50-51 0.2645173003296719 1101 52-53 0.41766178893711725 1101 54-55 0.39018090196477573 1101 56-57 0.23682182234512084 1101 58-59 0.21700409917625763 1101 60-61 0.19940182574266174 1101 62-63 0.2163149232034911 1101 64-65 0.2344723700503124 1101 66-67 0.18681109085789416 1101 68-69 0.02609513189049295 1101 70-71 0.16483170179987994 1101 72-73 0.10849190450510804 1101 74-75 0.0796658110309707 1101 76-77 0.06803412366746997 1101 78-79 0.13510393547413102 1101 80-81 0.09659848261003745 1101 82-83 0.22460841483801985 1101 84-85 0.20916272064053842 1101 86-87 0.09433195876581024 1101 88-89 0.3948968847654655 1101 90-91 0.48756232345500905 1101 92-93 0.40949476898949655 1101 94-95 0.39691499688589005 1101 96-97 0.33020037325248097 1101 98-99 0.21254476371536768 1101 100-101 0.7681586615204914 1102 1 0.06258058403478017 1102 2 0.05156420973799669 1102 3 0.11330622547175295 1102 4 0.01002504869088483 1102 5 0.0557844715237934 1102 6 0.023869971817674696 1102 7 0.1986566549727513 1102 8 0.181494129456226 1102 9 0.08550988419646899 1102 10-11 0.16783891160771702 1102 12-13 0.13125758188052572 1102 14-15 0.2708523545679853 1102 16-17 0.2923005073963978 1102 18-19 0.29215707641154864 1102 20-21 0.30817114927344136 1102 22-23 0.3391926818641835 1102 24-25 0.24196750561470282 1102 26-27 0.1814360882325161 1102 28-29 0.2803164506007292 1102 30-31 0.21293380392204853 1102 32-33 0.34740343414014774 1102 34-35 0.38279362696183483 1102 36-37 0.4438082858109098 1102 38-39 0.4214565576432179 1102 40-41 0.45410256632290924 1102 42-43 0.24464635233371013 1102 44-45 0.19795396279759103 1102 46-47 0.2499117872132075 1102 48-49 0.21947537612008006 1102 50-51 0.304003623810452 1102 52-53 0.39134282237910867 1102 54-55 0.4375644901417175 1102 56-57 0.2098296592452087 1102 58-59 0.2059849347273328 1102 60-61 0.05353863817672533 1102 62-63 0.1441647885641828 1102 64-65 0.09656025041292082 1102 66-67 0.15088759118070527 1102 68-69 0.1257597146661098 1102 70-71 0.05869629612350735 1102 72-73 0.2995721159386875 1102 74-75 0.3996454445391002 1102 76-77 0.32922125918337386 1102 78-79 0.25975290218961433 1102 80-81 0.37736080879934875 1102 82-83 0.43179810141451114 1102 84-85 0.45077597775700085 1102 86-87 0.5086601532343131 1102 88-89 0.6380533760598368 1102 90-91 0.5312531727062257 1102 92-93 0.641953602955347 1102 94-95 0.5738147758989562 1102 96-97 0.6358735960398576 1102 98-99 0.6993616595050689 1102 100-101 1.0176481869497387 1103 1 -0.0041522541934782 1103 2 0.12019568224936705 1103 3 -0.0037224857273656653 1103 4 0.07605354803971665 1103 5 0.07716404729993798 1103 6 0.05393329901494326 1103 7 0.06425453275624449 1103 8 0.11696799464348828 1103 9 0.15059940571024555 1103 10-11 0.12606009511647898 1103 12-13 0.08271495988304167 1103 14-15 0.20049569491516905 1103 16-17 0.053770596415724015 1103 18-19 0.004940637167429429 1103 20-21 0.11550914500098486 1103 22-23 0.013404299193361169 1103 24-25 0.12273082496253807 1103 26-27 0.09386764835368666 1103 28-29 0.22158011642083864 1103 30-31 0.10147671288804361 1103 32-33 0.11505305234543073 1103 34-35 0.07497749106030227 1103 36-37 0.1140595267386928 1103 38-39 0.17081341057776456 1103 40-41 0.16305868461807904 1103 42-43 0.26984218723904263 1103 44-45 0.34297750687552764 1103 46-47 0.3524850928962806 1103 48-49 0.6125739101330225 1103 50-51 0.6160394628008632 1103 52-53 0.5946119000876493 1103 54-55 0.5810337621448198 1103 56-57 0.6102963206789269 1103 58-59 0.1925811843865617 1103 60-61 0.43682348227264356 1103 62-63 0.4478804399109535 1103 64-65 0.3993088600927024 1103 66-67 0.2916715975817894 1103 68-69 0.47820894867120245 1103 70-71 0.3788107205955029 1103 72-73 0.3637389070990409 1103 74-75 0.41786245856426163 1103 76-77 0.39723089626106045 1103 78-79 0.4546876548354213 1103 80-81 0.4363564696667197 1103 82-83 0.5249109416515445 1103 84-85 0.48037192609929136 1103 86-87 0.6091067268725432 1103 88-89 0.7028797727735423 1103 90-91 0.6737009634285336 1103 92-93 0.6727335073948311 1103 94-95 0.5466781054594954 1103 96-97 0.6998169315760947 1103 98-99 0.9826356578794737 1103 100-101 1.555307950335763 1104 1 -0.03800262722036862 1104 2 0.034818768091369634 1104 3 0.042422563678996994 1104 4 0.023683985274722374 1104 5 0.07368159416400033 1104 6 0.041863835661338555 1104 7 -0.0885311243098883 1104 8 -0.03933839770041203 1104 9 -0.1961728599368655 1104 10-11 0.1254754779399292 1104 12-13 0.03702013289999684 1104 14-15 0.063457641244419 1104 16-17 0.15935966694568293 1104 18-19 0.132900325257566 1104 20-21 0.05592767469794779 1104 22-23 0.015761501713633663 1104 24-25 -0.0021381910601689924 1104 26-27 0.10706052966722979 1104 28-29 0.10530931017856915 1104 30-31 0.07525955458772415 1104 32-33 -0.025427950548937872 1104 34-35 -0.016643892040896446 1104 36-37 -0.02018555960585644 1104 38-39 0.020305337322035655 1104 40-41 0.0638163044951412 1104 42-43 0.23383437116600447 1104 44-45 0.20989228797701998 1104 46-47 0.005983449411353092 1104 48-49 -0.02181330884449295 1104 50-51 -0.002700541589362615 1104 52-53 -0.11649782182517043 1104 54-55 -0.24754980268009064 1104 56-57 -0.10709452329162161 1104 58-59 -0.1115726723559618 1104 60-61 -0.07288368785034294 1104 62-63 -0.13177797749715836 1104 64-65 0.034509981840912474 1104 66-67 -0.035594898661464924 1104 68-69 0.1068583261995002 1104 70-71 0.09802159533023058 1104 72-73 0.08465231900308012 1104 74-75 -0.02385990386743586 1104 76-77 -0.006436166394994558 1104 78-79 0.17266739217920346 1104 80-81 -0.0413166928821056 1104 82-83 0.06331546415716716 1104 84-85 0.08781910590684916 1104 86-87 0.23928657556033883 1104 88-89 0.3649368351567368 1104 90-91 0.40738757848489726 1104 92-93 0.4198496199817967 1104 94-95 0.233096864034799 1104 96-97 0.07685224848096084 1104 98-99 0.16047860579987727 1104 100-101 0.6416266459084348 1105 1 0.010189519007585801 1105 2 0.05123545876497104 1105 3 0.03731688657610022 1105 4 0.059109710253721914 1105 5 -0.003132489255335713 1105 6 -0.018293285159785455 1105 7 -0.023628126502316604 1105 8 0.04096631410091334 1105 9 0.1892767909395161 1105 10-11 0.14096572383171235 1105 12-13 0.004372931905841426 1105 14-15 -0.004862647308797818 1105 16-17 -0.025115484763958307 1105 18-19 -0.04305996220976027 1105 20-21 0.10851035477932669 1105 22-23 -0.016993882423641082 1105 24-25 0.08972935387534164 1105 26-27 0.09960480675869832 1105 28-29 0.0015274381888090716 1105 30-31 -0.07527688388544362 1105 32-33 0.08170840923821032 1105 34-35 0.0643038409144836 1105 36-37 0.11898091156130874 1105 38-39 0.1782494848368401 1105 40-41 0.24310616117070794 1105 42-43 0.15633452006976967 1105 44-45 0.27941555687453246 1105 46-47 0.1698647040278729 1105 48-49 0.11941856895960967 1105 50-51 0.05526698482940162 1105 52-53 0.006731894747147749 1105 54-55 0.22566433470926484 1105 56-57 0.12274340053204469 1105 58-59 0.1874042068399362 1105 60-61 0.2135307783833511 1105 62-63 0.18516419152218333 1105 64-65 0.25640313012029026 1105 66-67 0.17300144734145562 1105 68-69 0.11709378863381659 1105 70-71 0.12772455359220203 1105 72-73 -0.09302897183656356 1105 74-75 -0.08408327166945995 1105 76-77 -0.009702730064827136 1105 78-79 -0.06437666348122661 1105 80-81 -0.012285928756607944 1105 82-83 0.05472199493446439 1105 84-85 0.049211702533234813 1105 86-87 -0.06891625932422585 1105 88-89 0.20358755230562764 1105 90-91 0.2548945242883853 1105 92-93 0.1844027851085741 1105 94-95 0.22406782597742492 1105 96-97 0.08725120117089347 1105 98-99 0.2711408446561059 1105 100-101 0.5929239724882223 1106 1 0.06239685908592563 1106 2 -0.014552771743851167 1106 3 0.030677171109026347 1106 4 0.024928286884481565 1106 5 0.01619174504555332 1106 6 -0.08637989411276692 1106 7 0.09648406607305304 1106 8 0.045937393205804256 1106 9 0.03205585515799214 1106 10-11 0.03497929071007633 1106 12-13 0.10450532587056927 1106 14-15 0.11114861518596086 1106 16-17 -0.056895240777940614 1106 18-19 -0.10327239307024882 1106 20-21 0.025534729360963127 1106 22-23 0.06663630644293761 1106 24-25 0.1028926848706675 1106 26-27 0.03596482546280555 1106 28-29 0.03328377404271521 1106 30-31 -0.021608886473266864 1106 32-33 -0.009464072033672721 1106 34-35 0.13079127329627482 1106 36-37 0.1361128587414342 1106 38-39 0.050695886699564596 1106 40-41 0.1290157521466213 1106 42-43 0.03170624004632572 1106 44-45 0.16928956041677878 1106 46-47 0.09856132608013723 1106 48-49 -0.10060782976441374 1106 50-51 0.0773764166282973 1106 52-53 -0.031243594554041465 1106 54-55 -0.09883539465777602 1106 56-57 -0.13853270594040623 1106 58-59 -0.1682309238805928 1106 60-61 -0.24182238962367109 1106 62-63 -0.06572317232060954 1106 64-65 -0.16168295838468794 1106 66-67 -0.18320257307932764 1106 68-69 -0.1724490498415463 1106 70-71 -0.2354386572997349 1106 72-73 -0.24216034412182808 1106 74-75 0.09090435768188954 1106 76-77 0.1552126290763205 1106 78-79 -0.0010103177308060651 1106 80-81 -0.18962638341998073 1106 82-83 -0.013132414140869741 1106 84-85 -0.16070079014045646 1106 86-87 -0.10621676760175092 1106 88-89 0.20821313400635688 1106 90-91 0.34967250917777903 1106 92-93 0.2685314030228767 1106 94-95 0.42957372545569683 1106 96-97 0.46677009103128597 1106 98-99 0.5544088817824644 1106 100-101 1.0502126065444166 1107 1 -0.11501345921388051 1107 2 -0.14588361396243954 1107 3 -0.20112793499613701 1107 4 -0.11253134104906337 1107 5 -0.010235283915619675 1107 6 -0.10885093137277124 1107 7 -0.1102860430610022 1107 8 -0.0593175667909307 1107 9 -0.21578155173907732 1107 10-11 -0.15130738941132904 1107 12-13 -0.158754877394351 1107 14-15 -0.1446237271628874 1107 16-17 -0.1150996764478478 1107 18-19 -0.09191426733853092 1107 20-21 -0.252951510394972 1107 22-23 -0.17552370504485992 1107 24-25 -0.20918456584261946 1107 26-27 -0.26277944240997897 1107 28-29 -0.0931064924050844 1107 30-31 -0.29898048054607074 1107 32-33 -0.056145401855793864 1107 34-35 -0.045406509810383966 1107 36-37 -0.11604149516425366 1107 38-39 -0.13284929862744121 1107 40-41 -0.1411434056034011 1107 42-43 -0.29228626306888117 1107 44-45 -0.3245075010931444 1107 46-47 -0.35998021045952555 1107 48-49 -0.40448021323077654 1107 50-51 -0.08181193612861648 1107 52-53 -0.1873485258312897 1107 54-55 -0.4026471611730571 1107 56-57 -0.4801800431887955 1107 58-59 -0.24971324962549346 1107 60-61 -0.39342714983948923 1107 62-63 -0.34912703579789905 1107 64-65 -0.23660335204569094 1107 66-67 -0.32011311736854253 1107 68-69 -0.11451336893859221 1107 70-71 -0.2447189359572306 1107 72-73 -0.2623348032381472 1107 74-75 -0.18692470800883854 1107 76-77 -0.3175687905138247 1107 78-79 -0.25053381749069104 1107 80-81 -0.15240031711092428 1107 82-83 -0.06730160487907355 1107 84-85 -0.3097292318493139 1107 86-87 -0.23604650655259363 1107 88-89 -0.028817584859680778 1107 90-91 0.011279654837370146 1107 92-93 0.07907047408085077 1107 94-95 0.06830356296303464 1107 96-97 0.045489539303247284 1107 98-99 0.2586026724095518 1107 100-101 0.7605532306518406 1108 1 0.041951053777729896 1108 2 -0.026153781735132497 1108 3 -0.05416527770080393 1108 4 -0.020296291846101155 1108 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-0.7846007108364432 2316 50-51 -0.9178065293516013 2316 52-53 -0.9981458139845287 2316 54-55 -1.0256910269860882 2316 56-57 -1.0157076431263334 2316 58-59 -0.9750972869459176 2316 60-61 -0.9304164013489711 2316 62-63 -0.9638021086690216 2316 64-65 -1.092400347413168 2316 66-67 -1.0023032849721254 2316 68-69 -1.1132101469939713 2316 70-71 -1.1652033662612098 2316 72-73 -1.2328821824802887 2316 74-75 -1.2390043001908104 2316 76-77 -1.1834599002369153 2316 78-79 -1.0697184416839036 2316 80-81 -1.0791547544417561 2316 82-83 -1.372406649903013 2316 84-85 -1.4533227541270577 2316 86-87 -1.6488806076352525 2316 88-89 -1.9044007164961556 2316 90-91 -2.3032535795994384 2316 92-93 -2.4651919009972225 2316 94-95 -2.409117124628054 2316 96-97 -2.5693244441445984 2316 98-99 -3.1637116688875544 2316 100-101 -3.956312205013372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 29.0 5 94.0 6 233.0 7 506.0 8 949.0 9 1696.0 10 2883.0 11 4749.0 12 6941.0 13 9914.0 14 11630.0 15 16012.0 16 21335.0 17 30229.0 18 27096.0 19 14535.0 20 11540.0 21 11221.0 22 11583.0 23 12792.0 24 13989.0 25 16221.0 26 18549.0 27 22006.0 28 26760.0 29 31469.0 30 38239.0 31 46568.0 32 56406.0 33 69998.0 34 91976.0 35 128728.0 36 175039.0 37 301118.0 38 345698.0 39 48344.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08406732889789 23.45996685141511 16.502774929006986 18.953190890680016 2 14.156833654254505 24.717854669111116 40.66322206495412 20.46208961168026 3 17.98348828304817 30.58810008315474 32.98907802717372 18.43933360662337 4 10.63360471812509 20.73558451391966 41.439947587314016 27.190863180641234 5 9.693273930650296 41.82517591253572 36.00643358345313 12.475116573360859 6 23.98470395252374 42.25440926023009 19.09012855489596 14.670758232350206 7 22.58023080511368 37.65871155015067 23.942850013551826 15.818207631183828 8 23.817103818050295 34.98964714240849 21.964283409553392 19.228965629987822 9 25.302273326503133 18.48616576741133 24.014450364362816 32.19711054172272 10-11 21.97027571359122 29.413854329854523 29.072139350876753 19.54373060567751 12-13 24.8350887199025 28.465718182248402 29.113931830320077 17.585261267529027 14-15 21.575613983332104 29.249852496496793 30.023723480099317 19.150810040071786 16-17 17.14272631924745 32.46710543500541 30.989588855198154 19.400579390548994 18-19 16.90373699206679 30.892627547194856 34.72835452871517 17.47528093202318 20-21 18.804864152930975 28.946977020896853 34.308829214418154 17.93932961175401 22-23 18.21900119661702 29.03990382812966 34.39736166535655 18.343733309896766 24-25 18.408081436333767 29.941207814947816 34.40510556595929 17.245605182759128 26-27 17.964002484197962 29.157336327646167 35.65371067083486 17.224950517321012 28-29 16.844827596803366 30.22843892182778 35.41731327212374 17.50942020924511 30-31 17.83825941698262 29.69638378279711 34.847767820651256 17.61758897956901 32-33 17.560431617977404 30.037146139081685 34.98943203405841 17.4129902088825 34-35 18.19753908979882 29.607568475911194 34.78529575729936 17.40959667699063 36-37 18.155053431567055 29.19857106018839 34.960064248812316 17.68631125943224 38-39 17.464372316590808 30.360041674478072 34.74895429637438 17.42663171255674 40-41 17.389350859605635 30.182449920619163 34.95384356289956 17.474355656875645 42-43 17.133960981570056 30.336485096120157 33.896139336433464 18.633414585876316 44-45 17.741993970701667 29.839771857742974 33.649077627984305 18.769156543571054 46-47 18.587148452697107 30.489743137456156 32.79580862870815 18.127299781138582 48-49 18.278689632877427 30.460959039657396 32.61729865538761 18.643052672077566 50-51 17.451794070612298 30.842268551923013 32.92858012073147 18.777357256733218 52-53 17.687407004050797 31.76873762742865 31.959415814888814 18.584439553631736 54-55 17.596723469611643 30.752934538841497 32.148065837905406 19.502276153641446 56-57 18.251513287242783 30.8700149408114 31.436118658682666 19.44235311326315 58-59 18.456266634461766 30.381606575945952 31.898770897462725 19.263355892129557 60-61 18.016013373671505 31.177863377743225 32.10513942943714 18.700983819148135 62-63 17.45757909687681 30.96404801955888 32.36114602353777 19.217226860026535 64-65 17.356631821162605 31.357296813261982 31.762499485276447 19.523571880298967 66-67 17.65870375759535 30.750993583843787 30.952522164917244 20.637780493643614 68-69 17.40407331925491 30.63013036883342 31.494533752572103 20.471262559339575 70-71 18.173018151445504 30.6109084450148 31.112057603266752 20.104015800272947 72-73 17.951283490065375 29.76411218330673 31.736932321381712 20.547672005246184 74-75 17.35328227685428 31.52907619567976 30.55442026148571 20.563221265980243 76-77 17.505122400848744 30.39923812265893 31.292001610622478 20.803637865869845 78-79 17.48961579039267 30.0740297619194 31.437041809815874 20.999312637872055 80-81 17.91011661338122 30.419566021709976 30.628928034535356 21.04138933037344 82-83 17.647704148579642 30.252285218921916 31.010019746946536 21.08999088555191 84-85 17.31493153101217 30.085668436905955 30.717349471786083 21.88205056029579 86-87 17.2857888529692 30.372759772794982 30.7347846747408 21.606666699495015 88-89 17.647829592278747 29.726176694111174 30.459393589978774 22.16660012363131 90-91 17.78909964071421 29.19542971462064 30.748472519213117 22.266998125452034 92-93 18.0734957626728 29.933033697644934 30.45497874422204 21.538491795460228 94-95 17.666055246601825 29.31073424799481 30.575141518290515 22.448068987112855 96-97 17.43023496292299 29.18578698796899 30.681896752262404 22.70208129684562 98-99 17.752483736252255 29.392287434276636 29.726904249009735 23.128324580461378 100-101 17.649609407290427 28.719911178225267 30.499573314993718 23.130906099490588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8485.0 1 4969.0 2 1597.0 3 1703.0 4 1321.5 5 1313.5 6 2590.5 7 4771.5 8 6270.5 9 6424.0 10 5469.0 11 4541.5 12 4575.5 13 4808.5 14 5020.5 15 5080.5 16 5253.5 17 5678.0 18 6187.0 19 6834.5 20 7627.0 21 8454.5 22 9387.5 23 10590.5 24 11646.0 25 13512.5 26 17252.5 27 21029.0 28 23334.5 29 26654.5 30 31992.0 31 37267.5 32 44853.5 33 54572.5 34 63179.0 35 69116.0 36 76529.5 37 83284.5 38 80460.5 39 73996.0 40 68403.5 41 62478.5 42 56736.5 43 55036.5 44 54022.5 45 50981.0 46 48577.5 47 53700.5 48 74170.5 49 69493.0 50 45634.0 51 34267.0 52 24874.5 53 18238.5 54 13463.0 55 10478.5 56 8199.0 57 6326.5 58 4828.0 59 3599.5 60 2640.0 61 1988.5 62 1530.5 63 1107.5 64 795.5 65 578.0 66 429.5 67 341.5 68 207.5 69 126.5 70 77.0 71 66.5 72 45.0 73 28.5 74 33.0 75 40.0 76 33.0 77 22.5 78 27.0 79 30.0 80 21.5 81 9.5 82 3.0 83 1.5 84 0.5 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3997942334982702 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 4.3021670015186647E-4 16-17 0.001997434679276523 18-19 1.8437858577937137E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.5364882148280947E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007866819659919843 36-37 0.017608154941929962 38-39 0.011800229489879768 40-41 0.007375143431174854 42-43 0.003749031244180551 44-45 0.001966704914979961 46-47 0.0018437858577937136 48-49 6.145952859312378E-5 50-51 3.0729764296561895E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.380274072621808E-4 60-61 5.83865521634676E-4 62-63 0.0 64-65 0.0 66-67 0.001382839393345285 68-69 6.760548145243616E-4 70-71 0.0016286775077177804 72-73 0.0 74-75 0.0 76-77 0.10008684231390208 78-79 0.035585067055418675 80-81 6.145952859312378E-5 82-83 0.0 84-85 0.0 86-87 0.15211233326798138 88-89 0.3275485576370532 90-91 0.2804090992061273 92-93 0.0 94-95 3.072976429656189E-5 96-97 3.072976429656189E-5 98-99 1.2291905718624755E-4 100-101 5.224059930415522E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1627087.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.302938281993118 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6954705327975 20.715850098390288 2 12.559376718132048 7.360532816634493 3 5.273650152824206 4.636003348470939 4 2.8952436653086027 3.393565857434781 5 1.8633880257055873 2.730137215632791 6 1.2197002122444214 2.1444480025159316 7 0.8667252359627238 1.7778317267803132 8 0.6759947130674894 1.5846905084776233 9 0.49166120381517153 1.2966406119941771 >10 2.7987713903356077 15.376416356956074 >50 0.3241594898840755 6.700639573745655 >100 0.29949849525259326 17.13000559673364 >500 0.02459040038376138 4.777939914430224 >1k 0.010088369388209797 5.498634073732395 >5k 0.0010508718112718538 2.2324767202652898 >10k+ 6.305230867631123E-4 2.644187577805455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15888 0.9764689902875507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13607 0.8362798055666353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13439 0.8259546047629904 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9425 0.5792560569901917 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8971 0.5513534310089134 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7222 0.44386071549954 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5431 0.3337866997892553 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5200 0.31958954868424366 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4085 0.25106217430291067 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3723 0.22881382495219985 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3676 0.22592522710832302 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3616 0.2222376553927356 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3452 0.21215829270346329 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3298 0.20269352530012225 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3034 0.18646820975153758 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3005 0.18468588342233697 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2997 0.18419420719359197 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2873 0.17657322564804465 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2769 0.17018143467435976 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2765 0.16993559655998727 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2288 0.14061940142106724 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2046 0.12574619550153127 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1994 0.12255030001468883 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1939 0.11917002594206702 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1805 0.11093444911058842 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1768 0.10866044655264286 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 1719 0.10564892965157979 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.145952859312379E-5 0.0 10-11 0.0 0.0 0.0 9.218929288968568E-5 0.0 12-13 0.0 0.0 0.0 1.2291905718624758E-4 0.0 14-15 0.0 0.0 0.0 1.8437858577937137E-4 0.0 16-17 0.0 0.0 0.0 3.994869358553046E-4 0.0 18-19 0.0 0.0 0.0 7.375143431174855E-4 0.0 20-21 0.0 0.0 0.0 0.0015672179791246564 0.0 22-23 0.0 0.0 0.0 0.0034724633655114938 0.0 24-25 0.0 0.0 0.0 0.007436602959767978 0.0 26-27 0.0 0.0 0.0 0.01299869029744568 0.0 28-29 0.0 0.0 0.0 0.030760494060858453 0.0 30-31 0.0 0.0 0.0 0.07642492380554942 0.0 32-33 0.0 0.0 0.0 0.15918017905619058 0.0 34-35 0.0 0.0 0.0 0.2818841278923623 0.0 36-37 0.0 0.0 0.0 0.454800511589116 0.0 38-39 0.0 0.0 0.0 0.7070918764638892 0.0 40-41 0.0 0.0 0.0 0.9919875212573145 0.0 42-43 0.0 0.0 0.0 1.262839663767211 0.0 44-45 0.0 0.0 0.0 1.5741014463270864 0.0 46-47 0.0 0.0 0.0 1.9440878084576916 0.0 48-49 0.0 0.0 0.0 2.319236770990119 0.0 50-51 0.0 0.0 0.0 2.7237326584257633 0.0 52-53 0.0 0.0 0.0 3.104474438060165 0.0 54-55 0.0 0.0 0.0 3.5117360042824997 0.0 56-57 0.0 0.0 0.0 3.9939167358598526 0.0 58-59 0.0 0.0 0.0 4.49518065106537 0.0 60-61 0.0 0.0 0.0 5.0588259877929085 0.0 62-63 0.0 0.0 0.0 5.615526397789424 0.0 64-65 0.0 0.0 0.0 6.162301093918149 0.0 66-67 0.0 0.0 0.0 6.767032125510191 0.0 68-69 0.0 0.0 0.0 7.432423711823645 0.0 70-71 0.0 0.0 0.0 8.170429731169875 0.0 72-73 0.0 0.0 0.0 8.901122066613524 0.0 74-75 0.0 0.0 0.0 9.65606018608716 0.0 76-77 0.0 0.0 0.0 10.40497527175867 0.0 78-79 0.0 0.0 0.0 11.2734906000724 0.0 80-81 0.0 0.0 0.0 12.20438734990815 0.0 82-83 0.0 0.0 0.0 13.113066480157483 0.0 84-85 0.0 0.0 0.0 14.032163000503353 0.0 86-87 0.0 0.0 0.0 14.967177538754843 0.0 88-89 0.0 0.0 0.0 15.950222698601856 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGTGA 145 2.424349E-8 29.453764 7 ATGGGCG 165 2.6775524E-9 28.759567 5 CATTCCG 405 0.0 28.120468 9 CATGGGT 1545 0.0 26.414127 4 CATATAG 695 0.0 25.945683 3 GTACATG 13190 0.0 24.059568 1 TGCCGTT 50 0.0016636454 23.72701 94-95 GGACGTG 240 2.3283064E-10 23.726646 6 CATGGGG 4310 0.0 23.231194 4 ACATGGG 12510 0.0 23.176626 3 TAGGACG 250 3.8744474E-10 22.77758 4 TACATGG 13570 0.0 22.76499 2 GACATAT 670 0.0 22.757973 1 GAGTACT 13290 0.0 22.246897 12-13 CTTTGCG 320 0.0 22.24373 2 AGTACTT 14075 0.0 21.596188 12-13 TAGGCAT 550 0.0 21.569677 5 ATGGGGA 2300 0.0 21.250822 5 GTACTTT 14010 0.0 21.069712 14-15 TTAGGCA 635 0.0 20.924286 4 >>END_MODULE