##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938738_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626278 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64465607971085 33.0 31.0 34.0 30.0 34.0 2 31.677868728470777 33.0 31.0 34.0 30.0 34.0 3 31.647927968034985 33.0 31.0 34.0 28.0 34.0 4 35.231998465207056 37.0 35.0 37.0 33.0 37.0 5 35.30149826782383 37.0 35.0 37.0 33.0 37.0 6 35.39994515082907 37.0 35.0 37.0 33.0 37.0 7 35.347433833575806 37.0 35.0 37.0 33.0 37.0 8 35.325136907711965 37.0 35.0 37.0 33.0 37.0 9 36.873984644691745 39.0 37.0 39.0 33.0 39.0 10-11 36.7675083841754 39.0 37.0 39.0 32.5 39.0 12-13 36.691395321095165 39.0 37.0 39.0 32.0 39.0 14-15 37.762212549145964 40.0 37.5 41.0 32.0 41.0 16-17 37.691588707465755 40.0 37.5 41.0 32.0 41.0 18-19 37.77235534146068 40.0 37.5 41.0 32.0 41.0 20-21 37.72918129618675 40.0 37.5 41.0 32.0 41.0 22-23 37.70794476713084 40.0 37.5 41.0 32.0 41.0 24-25 37.64149764062479 40.0 37.0 41.0 32.0 41.0 26-27 37.48568910112539 40.0 37.0 41.0 32.0 41.0 28-29 37.32825199627616 40.0 37.0 41.0 32.0 41.0 30-31 37.05298663574125 39.5 36.0 41.0 31.0 41.0 32-33 36.763041128269585 39.0 35.0 41.0 31.0 41.0 34-35 36.43863503041916 39.0 35.0 41.0 30.0 41.0 36-37 36.18281468482019 39.0 35.0 41.0 30.0 41.0 38-39 35.99050838786481 39.0 35.0 41.0 30.0 41.0 40-41 35.83745399003122 39.0 35.0 41.0 29.5 41.0 42-43 35.65977495852493 39.0 35.0 41.0 28.0 41.0 44-45 35.28907327037567 39.0 35.0 41.0 25.5 41.0 46-47 34.733370616831806 39.0 35.0 41.0 22.0 41.0 48-49 34.25979783284285 39.0 35.0 41.0 16.0 41.0 50-51 33.10866254109076 38.0 33.0 40.0 9.5 40.5 52-53 33.20546733092374 38.0 33.5 39.5 8.5 40.5 54-55 33.73409620003468 39.0 34.0 40.0 7.5 41.0 56-57 33.660444585735036 39.0 34.0 41.0 2.0 41.0 58-59 33.50839924047426 39.0 34.0 40.5 2.0 41.0 60-61 33.217951666320275 38.0 33.5 40.0 2.0 41.0 62-63 32.87767897001619 38.0 33.0 40.0 2.0 41.0 64-65 32.542602802226924 38.0 33.0 40.0 2.0 41.0 66-67 32.15676255843097 37.0 32.0 40.0 2.0 41.0 68-69 31.748067673546586 36.5 31.5 39.0 2.0 41.0 70-71 31.317933342269896 36.0 31.0 39.0 2.0 41.0 72-73 30.86769144020887 35.5 31.0 39.0 2.0 40.0 74-75 30.377631930088214 35.0 30.5 38.0 2.0 40.0 76-77 29.883271802237992 35.0 30.0 37.0 2.0 39.0 78-79 29.39021772415294 35.0 29.0 37.0 2.0 39.0 80-81 28.852560570824913 34.5 29.0 36.0 2.0 39.0 82-83 28.367088529759364 34.0 28.5 36.0 2.0 37.5 84-85 27.923768875924043 34.0 27.5 35.0 2.0 37.0 86-87 27.474452707347698 34.0 26.0 35.0 2.0 37.0 88-89 26.94726578112721 34.0 25.5 35.0 2.0 36.0 90-91 26.430013503226384 34.0 24.5 35.0 2.0 36.0 92-93 25.93674636193812 34.0 21.5 35.0 2.0 35.5 94-95 25.399295200451583 33.0 18.5 35.0 2.0 35.0 96-97 24.791754238820175 33.0 4.5 35.0 2.0 35.0 98-99 24.091525557131067 32.5 2.0 35.0 2.0 35.0 100-101 21.789006861065573 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07766551428244384 1101 2 0.14248617498231297 1101 3 0.2543767480351278 1101 4 0.006678299083226591 1101 5 0.11191194848581176 1101 6 0.10317874524450588 1101 7 0.19182981906300256 1101 8 0.2412594036147766 1101 9 0.3865513336221369 1101 10-11 0.27795672469920163 1101 12-13 0.17776850546410827 1101 14-15 0.19502017821481132 1101 16-17 0.3800222559447235 1101 18-19 0.42009740133782714 1101 20-21 0.4756861667376242 1101 22-23 0.4807686458826552 1101 24-25 0.5369287050141978 1101 26-27 0.4544208629149864 1101 28-29 0.45802844834408774 1101 30-31 0.5199311151556572 1101 32-33 0.517247166172659 1101 34-35 0.6215401699866376 1101 36-37 0.6443991892614633 1101 38-39 0.4872925409699391 1101 40-41 0.6067268259602585 1101 42-43 0.6516833957083819 1101 44-45 0.6657105692775289 1101 46-47 0.5518684061745134 1101 48-49 0.5862938361250158 1101 50-51 0.5722145472313827 1101 52-53 0.5430768167414115 1101 54-55 0.5420261066528269 1101 56-57 0.3937868889425502 1101 58-59 0.48064614197470945 1101 60-61 0.44214841970676133 1101 62-63 0.36996597410236376 1101 64-65 0.2076735906106748 1101 66-67 0.26371180968118324 1101 68-69 0.4094642809237321 1101 70-71 0.2862260165165402 1101 72-73 0.2616320923067441 1101 74-75 0.31317628399101594 1101 76-77 0.3411242605385958 1101 78-79 0.347913327224429 1101 80-81 0.253070858845895 1101 82-83 0.37517269832154554 1101 84-85 0.456603113293081 1101 86-87 0.2556053074666309 1101 88-89 0.29837947335643733 1101 90-91 0.2668786743916698 1101 92-93 0.07967003997949007 1101 94-95 -0.09179979673119831 1101 96-97 0.0564758259980529 1101 98-99 0.01479680773197245 1101 100-101 0.35516949342087756 1102 1 0.010493413830580778 1102 2 -0.053890226864826474 1102 3 0.05094708963694217 1102 4 0.15522077486555474 1102 5 0.08387996578662182 1102 6 0.16793899900301312 1102 7 0.07305163951744476 1102 8 0.14096502191554805 1102 9 0.27461560654645467 1102 10-11 0.23162942780273 1102 12-13 0.20050930572600834 1102 14-15 0.3827269522179577 1102 16-17 0.4925866751918804 1102 18-19 0.5178608940390461 1102 20-21 0.4395642674176443 1102 22-23 0.4761557565771213 1102 24-25 0.4425033569394614 1102 26-27 0.32454818858001033 1102 28-29 0.28844958529730036 1102 30-31 0.4696276812811817 1102 32-33 0.46337618323043017 1102 34-35 0.45921606739201337 1102 36-37 0.42388572012070114 1102 38-39 0.2978770138853406 1102 40-41 0.3734776902569905 1102 42-43 0.5829829656680587 1102 44-45 0.682945042936943 1102 46-47 0.7577706724423976 1102 48-49 0.6390531375450905 1102 50-51 0.7061762007289971 1102 52-53 0.5700969398509912 1102 54-55 0.5146339763411873 1102 56-57 0.5188377299268296 1102 58-59 0.5296165500418155 1102 60-61 0.5453649179781692 1102 62-63 0.4305194464175841 1102 64-65 0.5469416788408594 1102 66-67 0.5302996108210181 1102 68-69 0.5120198463994861 1102 70-71 0.5949981943459015 1102 72-73 0.4993775550589028 1102 74-75 0.4173965816959786 1102 76-77 0.3747995924930194 1102 78-79 0.25806986785505615 1102 80-81 0.26072552704027174 1102 82-83 0.36300992285021394 1102 84-85 0.44769416081576097 1102 86-87 0.2407324595282958 1102 88-89 0.33551695233802903 1102 90-91 0.3721536125144702 1102 92-93 0.21787629565260147 1102 94-95 0.2961820145966705 1102 96-97 0.31597874997466135 1102 98-99 -0.20401658849537796 1102 100-101 0.23197992847089566 1103 1 0.2797113908652342 1103 2 0.29663482447783096 1103 3 0.43678555723617407 1103 4 0.3864241951262102 1103 5 0.41599108550866504 1103 6 0.47292408057003144 1103 7 0.44374079362292207 1103 8 0.37384434406328637 1103 9 0.5318245679393598 1103 10-11 0.3974944708789039 1103 12-13 0.43959737614617467 1103 14-15 0.5275608797053337 1103 16-17 0.4825102747320571 1103 18-19 0.5823669712015729 1103 20-21 0.5002882634910648 1103 22-23 0.4210027619115877 1103 24-25 0.36130849378863417 1103 26-27 0.4239656592920866 1103 28-29 0.5336632547151581 1103 30-31 0.5021963641966636 1103 32-33 0.6280214345188355 1103 34-35 0.7982164678001737 1103 36-37 0.7893106371328784 1103 38-39 0.795126629622672 1103 40-41 0.6948439862231979 1103 42-43 0.6625011196572856 1103 44-45 0.7485425552398084 1103 46-47 0.8848671643502755 1103 48-49 1.026201004402921 1103 50-51 1.0903799374412557 1103 52-53 1.0613259022772112 1103 54-55 0.9361628573941232 1103 56-57 1.04332075132929 1103 58-59 1.0338555485047678 1103 60-61 1.0636198165199104 1103 62-63 0.9756138006625577 1103 64-65 1.0079002688874894 1103 66-67 1.0646426831140587 1103 68-69 1.0109590876906118 1103 70-71 0.9682254195173634 1103 72-73 1.1998286600562516 1103 74-75 1.0126719268865614 1103 76-77 1.010832613249665 1103 78-79 0.9039209612276551 1103 80-81 0.7892715610620478 1103 82-83 0.938837217770196 1103 84-85 1.0425287341227438 1103 86-87 1.1294407880179804 1103 88-89 1.1773993227543258 1103 90-91 1.0839493462301313 1103 92-93 1.1099178606026783 1103 94-95 1.2258546809275082 1103 96-97 1.299481288045726 1103 98-99 1.267920127641073 1103 100-101 1.6854994838886803 1104 1 0.2683060137257307 1104 2 0.38338201219305645 1104 3 0.29680583629644985 1104 4 0.37185579474869 1104 5 0.35965611114445295 1104 6 0.2692470660558399 1104 7 0.37332207566765874 1104 8 0.2653928224960538 1104 9 0.48115242550128556 1104 10-11 0.4408106711697002 1104 12-13 0.4398397045394091 1104 14-15 0.44855066365044394 1104 16-17 0.5617436879912461 1104 18-19 0.5551145925570822 1104 20-21 0.571477011801143 1104 22-23 0.43777284328291444 1104 24-25 0.4353956172230653 1104 26-27 0.5503080310493544 1104 28-29 0.556843878646994 1104 30-31 0.46846010676738814 1104 32-33 0.6841077034268253 1104 34-35 0.6858637779550065 1104 36-37 0.7197703596031033 1104 38-39 0.6702557651480134 1104 40-41 0.7433686527028058 1104 42-43 0.6135125218458981 1104 44-45 0.6298096138832747 1104 46-47 0.8582859715844151 1104 48-49 0.7560287599318372 1104 50-51 0.8198856512896882 1104 52-53 0.8413810001013573 1104 54-55 0.8514100961258606 1104 56-57 0.7973717805872553 1104 58-59 0.9541789293684744 1104 60-61 1.001299968549084 1104 62-63 1.0554211495899395 1104 64-65 0.9227919863580922 1104 66-67 0.7869726068443867 1104 68-69 0.7746713673630801 1104 70-71 0.841562600345366 1104 72-73 0.6740350345684085 1104 74-75 0.543194144573981 1104 76-77 0.6012067555346938 1104 78-79 0.3656745260058578 1104 80-81 0.2977498662937883 1104 82-83 0.37338623187625686 1104 84-85 0.46421202366691006 1104 86-87 0.2963364975300067 1104 88-89 0.4250477807804991 1104 90-91 0.41546415377036894 1104 92-93 0.48627298648590056 1104 94-95 0.6026019961839957 1104 96-97 0.49625513188421166 1104 98-99 0.5025545943910643 1104 100-101 0.6790490846845358 1105 1 0.13311893974195144 1105 2 0.21448990313290395 1105 3 0.15360530393165206 1105 4 0.2580317238993928 1105 5 0.2698195521116702 1105 6 0.26708588888284623 1105 7 0.2019298422232083 1105 8 0.23885854218360691 1105 9 0.1768275834693398 1105 10-11 0.28883016218915003 1105 12-13 0.30374617998595 1105 14-15 0.44512854065924756 1105 16-17 0.5528066761996158 1105 18-19 0.4406810386489255 1105 20-21 0.40380386885870934 1105 22-23 0.40119764053650897 1105 24-25 0.43594757254422944 1105 26-27 0.4442890748823771 1105 28-29 0.4580189992997745 1105 30-31 0.3877036014686155 1105 32-33 0.4784071710620381 1105 34-35 0.28125254107754927 1105 36-37 0.30940945856324475 1105 38-39 0.3064285846912753 1105 40-41 0.39835770688461025 1105 42-43 0.5789772215821998 1105 44-45 0.5939589496711264 1105 46-47 0.6889865039492094 1105 48-49 0.7823671349753738 1105 50-51 0.793772722785782 1105 52-53 0.8092338783540001 1105 54-55 0.7653299428844065 1105 56-57 0.5627347961601714 1105 58-59 0.624905706820087 1105 60-61 0.6548741513442522 1105 62-63 0.6565440288376578 1105 64-65 0.60367318691533 1105 66-67 0.5664205893310807 1105 68-69 0.7330041824285054 1105 70-71 0.5889754069526596 1105 72-73 0.5773604487681219 1105 74-75 0.6257464481711779 1105 76-77 0.6193964042734343 1105 78-79 0.5686976863620963 1105 80-81 0.8308827336006175 1105 82-83 0.6059195668680495 1105 84-85 0.6463650536684042 1105 86-87 0.5684846765438891 1105 88-89 0.6033837676455143 1105 90-91 0.6853905182661144 1105 92-93 0.6101613018249736 1105 94-95 0.7060244301359333 1105 96-97 0.5149267697766433 1105 98-99 0.8030965989684056 1105 100-101 1.066271396739861 1106 1 0.1448009734459248 1106 2 0.21431896474835455 1106 3 0.2790837821172296 1106 4 0.2710812646797649 1106 5 0.2797504004437883 1106 6 0.2961745260336528 1106 7 0.2548557997767489 1106 8 0.1381803124185268 1106 9 0.15776907327573753 1106 10-11 0.21288462659216378 1106 12-13 0.2768121875818821 1106 14-15 0.2690676029912922 1106 16-17 0.5052955092204314 1106 18-19 0.3195641018647777 1106 20-21 0.3631600954946421 1106 22-23 0.39243798139809627 1106 24-25 0.16267419951934414 1106 26-27 0.30836096156441073 1106 28-29 0.24491519118071636 1106 30-31 0.033443876642095915 1106 32-33 0.1911142379086428 1106 34-35 0.20360060745837671 1106 36-37 0.36556719662294057 1106 38-39 0.26579376879714545 1106 40-41 0.4011770705459412 1106 42-43 0.5218509570860306 1106 44-45 0.4308075923158512 1106 46-47 0.18858341780222787 1106 48-49 0.3073995311582962 1106 50-51 0.37496957513084084 1106 52-53 0.07585819609337108 1106 54-55 0.14209755028446835 1106 56-57 0.30462343390814794 1106 58-59 0.38705576832804667 1106 60-61 0.33278891206037287 1106 62-63 0.3082197475253281 1106 64-65 0.26836560232695916 1106 66-67 0.34705513650111186 1106 68-69 0.35810383428151127 1106 70-71 0.170951932409789 1106 72-73 0.19620218818934987 1106 74-75 0.062185361212058865 1106 76-77 0.09349252091163507 1106 78-79 0.34448027065188924 1106 80-81 0.2697748737193386 1106 82-83 0.36064687162506104 1106 84-85 0.5160745479893656 1106 86-87 0.3723627385056929 1106 88-89 0.44338320033908474 1106 90-91 0.7228693189206084 1106 92-93 0.8714869028712044 1106 94-95 0.9246673137246546 1106 96-97 0.987420245752709 1106 98-99 1.2045200155863043 1106 100-101 1.701857260060816 1107 1 -9.569678488077216E-4 1107 2 -0.04249662202234106 1107 3 0.12154618955026919 1107 4 0.23327849721060545 1107 5 0.11006915084203683 1107 6 -0.0062813043938589885 1107 7 0.011342170068019186 1107 8 -0.04371380658133006 1107 9 0.07183771289758312 1107 10-11 0.05596884668601376 1107 12-13 0.021199943804951715 1107 14-15 0.04003876597139566 1107 16-17 0.1710884155757384 1107 18-19 0.0654653750060632 1107 20-21 0.11960014110888295 1107 22-23 0.14781878489138478 1107 24-25 0.025573662885499004 1107 26-27 0.25580881975304237 1107 28-29 0.34358858426373473 1107 30-31 0.16378650347466817 1107 32-33 0.17824696378447413 1107 34-35 0.23939932911701334 1107 36-37 0.30089318685411115 1107 38-39 0.2863264037081521 1107 40-41 0.2637321200089815 1107 42-43 0.4897142652319175 1107 44-45 0.781780190811638 1107 46-47 0.868169176314801 1107 48-49 0.8513903965911496 1107 50-51 0.7574636444735816 1107 52-53 0.575980874091826 1107 54-55 0.6351885817537521 1107 56-57 0.6796442159272829 1107 58-59 0.6334492182750537 1107 60-61 0.6099868074186006 1107 62-63 0.6762165732778982 1107 64-65 0.6259194176859424 1107 66-67 0.42834926853780075 1107 68-69 0.637720866528614 1107 70-71 0.602809064908616 1107 72-73 0.6992592506701705 1107 74-75 0.8613926049999705 1107 76-77 0.6760447432876653 1107 78-79 0.6579027817350216 1107 80-81 0.760367485354486 1107 82-83 0.6944134230842742 1107 84-85 0.7203788245810152 1107 86-87 0.5666013768751235 1107 88-89 0.5447604531282728 1107 90-91 0.6385858719544899 1107 92-93 0.6710962375316569 1107 94-95 0.6111955483406497 1107 96-97 0.6308694603571752 1107 98-99 0.7219730665331952 1107 100-101 1.265223925189428 1108 1 0.11263952337926142 1108 2 0.15034842319543884 1108 3 0.03314542899679296 1108 4 0.00923677861538863 1108 5 0.10474008363850373 1108 6 0.17745587324969847 1108 7 0.07392046233785265 1108 8 0.02173373759788433 1108 9 0.21560429008737003 1108 10-11 0.2666669261286998 1108 12-13 0.16930115653623545 1108 14-15 0.29268911908551587 1108 16-17 0.3918927887582342 1108 18-19 0.3204263487062491 1108 20-21 0.3026476677122716 1108 22-23 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2316 72-73 -1.2963286344259117 2316 74-75 -1.356000530098079 2316 76-77 -1.246566852766552 2316 78-79 -1.0782227199642485 2316 80-81 -1.2419422631847716 2316 82-83 -1.228893021533647 2316 84-85 -1.131509930258563 2316 86-87 -1.1914978038470068 2316 88-89 -1.2036083244208378 2316 90-91 -1.5957818489706064 2316 92-93 -1.4554743867438873 2316 94-95 -1.2660342042989399 2316 96-97 -1.4344757954798837 2316 98-99 -1.7936127683969012 2316 100-101 -1.9933931590014282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8845.0 3 1631.0 4 1453.0 5 1527.0 6 1828.0 7 2260.0 8 2761.0 9 3330.0 10 4241.0 11 6120.0 12 8836.0 13 12327.0 14 13913.0 15 17687.0 16 22848.0 17 30826.0 18 25117.0 19 13869.0 20 12643.0 21 12389.0 22 13130.0 23 14175.0 24 16107.0 25 17983.0 26 20798.0 27 24016.0 28 27610.0 29 32539.0 30 38260.0 31 45710.0 32 55034.0 33 68656.0 34 89778.0 35 128101.0 36 168999.0 37 270696.0 38 322846.0 39 67367.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.463424800106544 22.60109355654975 16.044193594530736 18.89128804881297 2 13.970256261799099 24.01401879232662 41.727711444471474 20.2880135014028 3 18.038125785148306 30.709487579639223 32.76037658777858 18.492010047433894 4 10.376717580995543 19.882395476161584 42.52985845451124 27.21102848833163 5 9.73166048749218 41.31343669967363 36.16151530000585 12.793387512828335 6 23.87549051441091 41.88088902252007 19.57954235290421 14.664078110164807 7 22.637785325500687 37.595776288780534 24.38848033927768 15.377958046441098 8 23.556775323857117 34.4722426681363 22.376563187737876 19.594418820268704 9 25.705162549805706 18.468909539909227 24.36972305873224 31.456204851552826 10-11 21.860433589724234 29.101070803017535 29.411790078858303 19.626705528399928 12-13 24.946479741180095 28.349992297026656 29.255677091357263 17.44785087043599 14-15 21.827695395188496 28.874017006673867 30.337962137374674 18.960325460762963 16-17 17.09058414768165 32.20900229534603 31.288489598172603 19.411923958799708 18-19 16.961013786397892 30.665158642963576 35.05341516519177 17.32041240544676 20-21 18.94713136319553 28.66239958199987 34.591205237817675 17.799263816986922 22-23 18.312674819112786 28.72811052805967 34.68309484731501 18.276119805512533 24-25 18.440556718613387 29.528793617108484 34.74532628985648 17.28532337442165 26-27 18.016271339974374 28.760822903165195 35.97657504614862 17.246330710711817 28-29 16.93014587949643 29.849993584460577 35.70735023416719 17.512510301875807 30-31 17.798540830234295 29.402301615201022 35.13632691323305 17.66283064133163 32-33 17.495055727039166 29.730544824994297 35.24194591797128 17.532453529995255 34-35 18.178880976325985 29.312460789919758 35.05971349987055 17.44894473388371 36-37 18.20911943957882 28.86680303450337 35.180987862110015 17.743089663807798 38-39 17.545016821506838 30.050495385660053 34.95364054446125 17.450847248371865 40-41 17.43778686964267 29.982164216933228 35.17953176518718 17.400517148236926 42-43 17.14923973677047 30.0787454067237 34.08802053787859 18.683994318627235 44-45 17.815670288037747 29.592693754979166 33.88490898523312 18.70672697174997 46-47 18.60740273374353 30.260554817635704 33.049181886456516 18.082860562164253 48-49 18.36351477601185 30.257187015856296 32.80797019789649 18.571328010235366 50-51 17.5490114661346 30.605666661830067 33.24502939159809 18.600292480437243 52-53 17.80777344098513 31.445886943386487 32.25778268706959 18.488556928558793 54-55 17.68695953897123 30.462306798495014 32.54212864006638 19.308605022467372 56-57 18.371431613676805 30.547902292686768 31.845460844391425 19.235205249245006 58-59 18.673673154795626 29.941443926496667 32.356072022304254 19.02881089640345 60-61 18.268203724409148 30.575903149940242 32.701422678027534 18.454470447623088 62-63 17.70709900731251 30.399738748579768 32.99830143364186 18.89486081046586 64-65 17.656765065144064 30.787904163069506 32.46340779604455 19.09192297574188 66-67 17.941162938413182 30.29814396864691 31.60329773111924 20.15739536182066 68-69 17.608168140570875 30.225072243848544 32.18573848684109 19.981021128739496 70-71 18.552608662892744 30.09189207883981 31.841695374578027 19.51380388368942 72-73 18.33909297393835 29.428171435558514 32.40183806229057 19.830897528212567 74-75 17.821247817098143 31.11700353957382 31.337053632076987 19.724695011251054 76-77 18.034130514643458 30.08122049930078 31.77157004689241 20.113078939163344 78-79 17.983568394605427 29.893072042352692 31.98984389635392 20.133515666687963 80-81 18.544520151551396 30.29117313435139 31.13124937303712 20.033057341060086 82-83 18.43302253290261 29.955903484658826 31.627002440202684 19.984071542235878 84-85 18.16924030075448 29.887298874223518 31.357392745009726 20.586068080012275 86-87 18.190275961293004 30.156459659941703 31.427132988704543 20.22613139006075 88-89 18.6494335106227 29.56145038321113 31.1059022262889 20.683213879877272 90-91 18.817201810996465 29.162100733667856 31.34359004760921 20.67710740772647 92-93 19.559871701291755 29.730829606520164 30.997729947769038 19.711568744419043 94-95 19.163661067193676 29.150104990118574 31.0564476284585 20.62978631422925 96-97 19.028135424795106 28.92156030204056 31.15113669734771 20.89916757581662 98-99 19.40855385677615 29.208338878649137 30.261089422193017 21.122017842381695 100-101 19.773613469711457 28.311065847936934 30.965073918131758 20.95024676421985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9914.0 1 5883.5 2 1950.5 3 1993.5 4 1621.0 5 1623.0 6 2848.0 7 4970.5 8 6808.5 9 7141.5 10 5998.5 11 5074.0 12 5067.0 13 5202.5 14 5272.0 15 5369.5 16 5622.0 17 5963.0 18 6489.5 19 7159.5 20 7861.5 21 8721.5 22 9685.0 23 10821.5 24 12083.0 25 14005.5 26 17745.0 27 21637.0 28 23903.0 29 27155.5 30 32272.0 31 37159.5 32 44520.0 33 54287.0 34 63113.0 35 69116.0 36 76807.0 37 83369.0 38 79982.0 39 73766.0 40 68379.0 41 62710.5 42 57023.0 43 54527.5 44 53060.0 45 50047.0 46 47852.0 47 52052.5 48 72209.5 49 69690.0 50 44281.0 51 30076.5 52 21393.5 53 16633.5 54 13352.5 55 10709.5 56 8493.5 57 6585.5 58 5134.5 59 4016.0 60 3011.0 61 2327.0 62 1780.0 63 1331.0 64 1032.5 65 814.0 66 621.0 67 471.5 68 330.0 69 243.0 70 188.0 71 160.5 72 127.5 73 104.5 74 81.5 75 72.0 76 63.5 77 58.5 78 54.0 79 47.0 80 37.0 81 23.0 82 15.0 83 11.0 84 8.5 85 5.0 86 4.0 87 2.0 88 0.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2714173099556164 2 0.1686673496167322 3 0.1953540538579505 4 0.16608476533532399 5 0.23938096684576685 6 0.19904345997424794 7 0.24645232856867033 8 0.25284729917025256 9 0.15175757158370218 10-11 0.21094179469930727 12-13 0.21693707963829062 14-15 0.17564647618672824 16-17 0.29121712277974615 18-19 0.236460187003698 20-21 0.2036552176196198 22-23 0.24998800943012203 24-25 0.2384893603676616 26-27 0.3504013458953512 28-29 0.32060939150624923 30-31 0.2956444101193031 32-33 0.1960304449792717 34-35 0.36890986657877683 36-37 0.4082327867683139 38-39 0.3891093650655054 40-41 0.34800323191975785 42-43 0.3841286668085038 44-45 0.3937518677618464 46-47 0.39873256601884793 48-49 0.4178252426706873 50-51 0.4109076062026295 52-53 0.4103541952851849 54-55 0.40558871238496735 56-57 0.3834215306362135 58-59 0.3554127892033219 60-61 0.32070162665915664 62-63 0.3924605756211423 64-65 0.4269872678594927 66-67 0.4331055330023526 68-69 0.43713313467931064 70-71 0.43633376335411284 72-73 0.4282170698982585 74-75 0.42268296072381234 76-77 0.4066340441179183 78-79 0.379301693806348 80-81 0.3914152438881913 82-83 0.4018685612177008 84-85 0.4028524028487134 86-87 0.397687234285897 88-89 0.40641882876113433 90-91 0.427263973318215 92-93 0.4262493866362332 94-95 0.43522694151922364 96-97 0.43605705789539057 98-99 0.4331055330023526 100-101 0.4067262792708258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1626278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.869032231577766 #Duplication Level Percentage of deduplicated Percentage of total 1 72.15005592314704 22.27202401801764 2 12.042328756366404 7.4347006904706054 3 5.150320674663695 4.769562447274648 4 2.70497368544664 3.339996795264881 5 1.7021398965801304 2.627170566509324 6 1.1526666735164603 2.134902281822708 7 0.8249618686341226 1.7826042158882542 8 0.5733149539197049 1.4158142233114313 9 0.45021516965864383 1.2507935925004134 >10 2.6336356594714614 15.12995344197175 >50 0.3096370010998696 6.777103900667182 >100 0.27415493568641525 16.641536886555 >500 0.02079657840623182 4.273670021938736 >1k 0.009198486602756382 5.1834776663526805 >5k 9.998355002996066E-4 2.253253846532479 >10k+ 5.999013001797641E-4 2.713435404922191 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17475 1.074539531371635 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13778 0.8472106245057733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12705 0.781231745125987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9147 0.5624499624295477 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8646 0.531643421358464 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7560 0.4648651706534799 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5713 0.3512929523734565 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5437 0.3343216842384881 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4476 0.27522969627579047 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3722 0.2288661594143191 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3523 0.21662962912859918 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3293 0.20248690568279223 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3062 0.18828269213504703 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3042 0.1870528900962812 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3027 0.18613053856720685 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2710 0.16663817625276858 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2707 0.16645370594695372 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2704 0.16626923564113882 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2674 0.1644245325829901 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2584 0.15889042340854392 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2119 0.13029752600723862 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2111 0.1298056051917323 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2097 0.12894474376459622 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1774 0.10908344083852821 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1741 0.10705426747456462 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1729 0.10631638625130513 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1723 0.10594744563967538 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1646 0.10121270779042696 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.149010193829099E-5 0.0 16-17 0.0 0.0 0.0 9.223515290743648E-5 0.0 18-19 0.0 0.0 0.0 1.5372525484572748E-4 0.0 20-21 0.0 0.0 0.0 7.071361722903464E-4 0.0 22-23 0.0 0.0 0.0 0.002920779842068822 0.0 24-25 0.0 0.0 0.0 0.0070406166719343185 0.0 26-27 0.0 0.0 0.0 0.012759196152195382 0.0 28-29 0.0 0.0 0.0 0.030160895000731734 0.0 30-31 0.0 0.0 0.0 0.07461823870211612 0.0 32-33 0.0 0.0 0.0 0.15455537122189442 0.0 34-35 0.0 0.0 0.0 0.27237040653565997 0.0 36-37 0.0 0.0 0.0 0.437625055494817 0.0 38-39 0.0 0.0 0.0 0.6680284674575934 0.0 40-41 0.0 0.0 0.0 0.9327126112509669 0.0 42-43 0.0 0.0 0.0 1.18519096980959 0.0 44-45 0.0 0.0 0.0 1.4790521669726826 0.0 46-47 0.0 0.0 0.0 1.83102151046746 0.0 48-49 0.0 0.0 0.0 2.20232949102183 0.0 50-51 0.0 0.0 0.0 2.6032142106085185 0.0 52-53 0.0 0.0 0.0 2.9734153693279994 0.0 54-55 0.0 0.0 0.0 3.365537749388481 0.0 56-57 0.0 0.0 0.0 3.832093897845264 0.0 58-59 0.0 0.0 0.0 4.326013141664586 0.0 60-61 0.0 0.0 0.0 4.866326667396349 0.0 62-63 0.0 0.0 0.0 5.397078482276708 0.0 64-65 0.0 0.0 0.0 5.934317502911556 0.0 66-67 0.0 0.0 0.0 6.531632353140115 0.0 68-69 0.0 0.0 0.0 7.193511810403879 0.0 70-71 0.0 0.0 0.0 7.936373731920373 0.0 72-73 0.0 0.0 0.0 8.657683372707496 0.0 74-75 0.0 0.0 0.0 9.396855888107691 0.0 76-77 0.0 0.0 0.0 10.13811906697379 0.0 78-79 0.0 0.0 0.0 11.013092472504702 0.0 80-81 0.0 0.0 0.0 11.936427843210078 0.0 82-83 0.0 0.0 0.0 12.822623192344729 0.0 84-85 0.0 0.0 0.0 13.73070901776941 0.0 86-87 0.0 6.149010193829099E-5 0.0 14.640916251710962 0.0 88-89 0.0 6.149010193829099E-5 0.0 15.592875264868614 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 400 0.0 34.397182 9 GCGGCAC 65 0.0058792057 29.19565 3 GTAAGCG 110 1.8189894E-12 28.075153 94-95 CATGGGT 1500 0.0 26.251753 4 GAGTACT 12650 0.0 24.54408 12-13 GTACATG 12885 0.0 24.307295 1 TAGGCAT 640 0.0 23.721464 5 CCTTTAG 645 0.0 23.579027 1 CATGGGG 4530 0.0 23.250177 4 AGTACTT 13380 0.0 23.098536 12-13 TTAGGCA 770 0.0 22.79725 4 TACATGG 13515 0.0 22.64202 2 GTACTTT 13595 0.0 22.593208 14-15 CTTTGCG 295 5.456968E-12 22.515287 2 ACATGGG 12585 0.0 22.505703 3 CGTACGT 65 2.9204186E-4 21.948181 34-35 ACTCCCG 260 6.348273E-10 21.896736 6 AGGCTTA 790 0.0 21.019018 6 TAGGGTC 415 0.0 20.577656 4 ATGGGGA 2345 0.0 20.433842 5 >>END_MODULE