##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938738_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626278 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99037556924462 31.0 31.0 34.0 30.0 34.0 2 32.179169244126776 33.0 31.0 34.0 30.0 34.0 3 32.22498859358609 34.0 31.0 34.0 30.0 34.0 4 35.88241063336035 37.0 35.0 37.0 35.0 37.0 5 35.805956300214355 37.0 35.0 37.0 35.0 37.0 6 35.857599992129266 37.0 35.0 37.0 35.0 37.0 7 35.789108627184284 37.0 35.0 37.0 33.0 37.0 8 35.78826867239181 37.0 35.0 37.0 33.0 37.0 9 37.386994720459846 39.0 37.0 39.0 34.0 39.0 10-11 37.322210901211236 39.0 37.0 39.0 34.0 39.0 12-13 37.271633140213424 39.0 37.0 39.0 33.5 39.0 14-15 38.37827019734633 40.0 38.0 41.0 33.0 41.0 16-17 38.331489757593715 40.0 38.0 41.0 33.0 41.0 18-19 38.55526761107265 40.0 38.0 41.0 34.0 41.0 20-21 38.59510305126184 40.0 38.0 41.0 34.0 41.0 22-23 38.50654869585643 40.0 38.0 41.0 34.0 41.0 24-25 38.41232556795333 40.0 38.0 41.0 34.0 41.0 26-27 38.20347966337859 40.0 37.5 41.0 33.5 41.0 28-29 38.03119608086686 40.0 37.0 41.0 33.0 41.0 30-31 37.737143956937246 40.0 36.5 41.0 33.0 41.0 32-33 37.44043638295544 40.0 36.0 41.0 33.0 41.0 34-35 37.302610316317384 40.0 35.0 41.0 32.0 41.0 36-37 37.10940810857676 40.0 35.0 41.0 31.5 41.0 38-39 36.931068980826154 40.0 35.0 41.0 31.0 41.0 40-41 36.72527267785705 40.0 35.0 41.0 31.0 41.0 42-43 36.42573717408709 39.0 35.0 41.0 30.0 41.0 44-45 36.00156922740147 39.0 35.0 41.0 29.5 41.0 46-47 35.432127225480514 39.0 35.0 41.0 26.0 41.0 48-49 34.92478499985857 39.0 35.0 41.0 23.5 41.0 50-51 34.64627080978775 39.0 35.0 41.0 22.5 41.0 52-53 34.46130704590482 39.0 35.0 40.5 20.5 41.0 54-55 34.23916636639 39.0 34.0 40.0 19.5 41.0 56-57 33.984578589884386 38.0 34.0 40.0 16.5 41.0 58-59 33.72302367737865 38.0 34.0 40.0 13.0 41.0 60-61 33.36435775433229 38.0 33.0 40.0 8.5 41.0 62-63 33.048341058539805 38.0 33.0 40.0 7.5 41.0 64-65 32.68512640520255 37.0 32.5 40.0 2.0 41.0 66-67 32.28702534253061 37.0 32.0 39.0 2.0 41.0 68-69 31.951716127254997 36.0 32.0 39.0 2.0 40.0 70-71 31.52895691880478 36.0 31.0 39.0 2.0 40.0 72-73 31.031880465701434 35.0 31.0 38.0 2.0 40.0 74-75 30.53122098435815 35.0 30.5 37.5 2.0 39.5 76-77 28.56822818730869 33.0 27.5 35.5 2.0 38.5 78-79 29.44743641616009 34.0 29.0 36.0 2.0 39.0 80-81 29.431179663009644 34.0 29.0 36.0 2.0 38.5 82-83 29.06491141121014 34.0 29.0 36.0 2.0 37.0 84-85 28.621001452396207 34.0 29.0 35.0 2.0 37.0 86-87 28.064697733105902 34.0 28.5 35.0 2.0 36.5 88-89 27.432295093458805 34.0 26.5 35.0 2.0 36.0 90-91 26.890075682017468 33.0 25.5 35.0 2.0 35.5 92-93 26.586942392383097 33.0 25.0 35.0 2.0 35.0 94-95 26.2473098080402 33.0 25.0 35.0 2.0 35.0 96-97 25.664717840369235 33.0 22.5 35.0 2.0 35.0 98-99 24.917487354560535 32.5 10.0 35.0 2.0 35.0 100-101 21.519384754636047 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1682040556662514 1101 2 0.12632422161718893 1101 3 0.0865028353976669 1101 4 0.08492824850772962 1101 5 -0.0594790855562195 1101 6 0.13446887080274195 1101 7 0.1686371650040286 1101 8 0.06670575542926116 1101 9 0.22779093028134412 1101 10-11 0.20984150760528308 1101 12-13 0.08211862324391461 1101 14-15 0.18608944735602506 1101 16-17 0.20258417825038322 1101 18-19 0.26611637968993307 1101 20-21 0.22726052882517678 1101 22-23 0.13662539033801124 1101 24-25 0.1293847410469553 1101 26-27 0.05396494566575427 1101 28-29 0.035193927787773305 1101 30-31 0.12027238885197988 1101 32-33 0.16097645405233862 1101 34-35 0.2084798134140584 1101 36-37 0.28017304132708176 1101 38-39 0.27566152933695065 1101 40-41 0.3937238513584518 1101 42-43 0.3528608919803631 1101 44-45 0.3695744484618402 1101 46-47 0.18875296130191543 1101 48-49 0.02349574943311694 1101 50-51 0.10953514221677096 1101 52-53 0.07384733810837929 1101 54-55 0.3497643000744972 1101 56-57 0.27162987659212234 1101 58-59 0.25781215370565747 1101 60-61 0.28584081992478616 1101 62-63 0.1439688570982014 1101 64-65 0.23116829558532004 1101 66-67 0.17560528965034905 1101 68-69 0.2008517776060863 1101 70-71 0.07679095858267004 1101 72-73 0.019537043120141817 1101 74-75 -0.011261152473210245 1101 76-77 -0.02091274418021527 1101 78-79 -0.0019374297846894706 1101 80-81 0.22955275219179327 1101 82-83 0.2452674758370037 1101 84-85 0.31171405052341683 1101 86-87 0.12218012167632608 1101 88-89 0.17823086150733758 1101 90-91 -0.05558468024364416 1101 92-93 -0.04642230493535848 1101 94-95 0.018238548523285658 1101 96-97 -0.12941634536971947 1101 98-99 -0.15055959045974632 1101 100-101 0.1758938543073576 1102 1 0.16129960199602777 1102 2 0.1513243456195923 1102 3 0.18633468814233112 1102 4 0.1413171001958986 1102 5 -0.03674324539035467 1102 6 0.010772756045959397 1102 7 0.0736947269184256 1102 8 0.08717731175858745 1102 9 0.20473144384968833 1102 10-11 0.10310458117839261 1102 12-13 0.10368512077130987 1102 14-15 0.24141125838628597 1102 16-17 0.2501955200059527 1102 18-19 0.1402322425730418 1102 20-21 0.16691848060312253 1102 22-23 0.1392492811365429 1102 24-25 0.14166779884696012 1102 26-27 0.1490239460080076 1102 28-29 0.04347108803359134 1102 30-31 0.18288492103060605 1102 32-33 0.13078992964121738 1102 34-35 0.15954226598313426 1102 36-37 0.25391739301772986 1102 38-39 0.14018890658697103 1102 40-41 0.17334926490645586 1102 42-43 0.20658208002256373 1102 44-45 0.2711723433970903 1102 46-47 0.32956512742544675 1102 48-49 0.46195893808044275 1102 50-51 0.42017231615245265 1102 52-53 0.33610745546425846 1102 54-55 0.33377047043395436 1102 56-57 0.3677410373041923 1102 58-59 0.422370159548656 1102 60-61 0.48150792803999565 1102 62-63 0.4728300438507773 1102 64-65 0.5575934551764163 1102 66-67 0.532327955801172 1102 68-69 0.6245450403076482 1102 70-71 0.5988218451006304 1102 72-73 0.5589487261303283 1102 74-75 0.4633288955901165 1102 76-77 0.5510074081939358 1102 78-79 0.3407109638882133 1102 80-81 0.6132992615242152 1102 82-83 0.5837487688348375 1102 84-85 0.5246528330182478 1102 86-87 0.4340498794748342 1102 88-89 0.4784468736893288 1102 90-91 0.4532046099170124 1102 92-93 0.34759904331794544 1102 94-95 0.3763835222099772 1102 96-97 0.4588863841711266 1102 98-99 0.5300585863081579 1102 100-101 1.425497046625157 1103 1 0.17990243585247612 1103 2 0.1751841510987262 1103 3 0.10401206249812844 1103 4 0.010512796454023032 1103 5 -0.04572336706276303 1103 6 -0.03653675946016932 1103 7 0.03528783473154817 1103 8 0.04366521555729719 1103 9 0.13082061862139227 1103 10-11 0.12766671548303776 1103 12-13 0.18102444085242553 1103 14-15 0.22771261719074687 1103 16-17 0.06685056191965089 1103 18-19 -0.0250200791484545 1103 20-21 0.1401444141353636 1103 22-23 0.13879074921367618 1103 24-25 0.2220134092110726 1103 26-27 0.3358256535550552 1103 28-29 0.25063064422636216 1103 30-31 0.2657556860685162 1103 32-33 0.3662089374039752 1103 34-35 0.25850281932137875 1103 36-37 0.3524670436293178 1103 38-39 0.3809601443648347 1103 40-41 0.47516579401354875 1103 42-43 0.5813899486886953 1103 44-45 0.6833634801687722 1103 46-47 0.7646489548107809 1103 48-49 0.6483279464890117 1103 50-51 0.6558722554454093 1103 52-53 0.5874144328993225 1103 54-55 0.5228575721247068 1103 56-57 0.46448885120481265 1103 58-59 0.5722705418738556 1103 60-61 0.6638938440059476 1103 62-63 0.5675071986279008 1103 64-65 0.5664088928483579 1103 66-67 0.7170396296364316 1103 68-69 0.5617423123656202 1103 70-71 0.7087976591562466 1103 72-73 0.7473835500153179 1103 74-75 0.7338272100009355 1103 76-77 0.7139485029873391 1103 78-79 0.5318323973494188 1103 80-81 0.7041668878788236 1103 82-83 0.7193854178060484 1103 84-85 0.6866058238088577 1103 86-87 0.7565843016600304 1103 88-89 0.3754555987122572 1103 90-91 0.3744783922241375 1103 92-93 0.37808657254128164 1103 94-95 0.7483982210642921 1103 96-97 1.0158024663710457 1103 98-99 1.0785200825430294 1103 100-101 1.7769536927547627 1104 1 0.09194679470256517 1104 2 0.18211283002636236 1104 3 0.18275284764212074 1104 4 0.06673298368458802 1104 5 0.024963187537643705 1104 6 0.06303470190758276 1104 7 -0.003344412800522889 1104 8 0.03700437327481865 1104 9 0.09517283656389708 1104 10-11 0.19327393941997428 1104 12-13 0.013819443785870078 1104 14-15 0.07561647658926063 1104 16-17 0.18910364204106855 1104 18-19 0.13791547425307016 1104 20-21 0.1078860410516711 1104 22-23 0.13509524121683825 1104 24-25 0.1467704431115422 1104 26-27 0.14117058889164014 1104 28-29 0.05723823803295147 1104 30-31 0.09935579320316634 1104 32-33 0.25020059620559465 1104 34-35 0.24830430135081372 1104 36-37 0.44240435710022297 1104 38-39 0.4560038272462421 1104 40-41 0.48541851701943983 1104 42-43 0.5762340932694698 1104 44-45 0.5708370153574052 1104 46-47 0.6941760318201915 1104 48-49 0.6711849344746312 1104 50-51 0.5764634729542806 1104 52-53 0.534473650306154 1104 54-55 0.5790634109635775 1104 56-57 0.48355308098314254 1104 58-59 0.7642137605050934 1104 60-61 0.7774884668664441 1104 62-63 0.8293350561898194 1104 64-65 0.7177477572209838 1104 66-67 0.6064661403626559 1104 68-69 0.5771923417001084 1104 70-71 0.6764659498482928 1104 72-73 0.7636386835510187 1104 74-75 0.5704436679250762 1104 76-77 0.506708304533138 1104 78-79 0.5615255968494566 1104 80-81 0.5922797236311901 1104 82-83 0.6602668554511268 1104 84-85 0.7254628628109749 1104 86-87 0.7908785521000468 1104 88-89 0.8577991055867322 1104 90-91 0.8556150399296705 1104 92-93 1.1266453563078294 1104 94-95 1.101556780358223 1104 96-97 1.0835715146581677 1104 98-99 1.26930267571192 1104 100-101 2.080320144301833 1105 1 0.03846465628772222 1105 2 0.040949090536834376 1105 3 0.02349741204831446 1105 4 0.12174815856891286 1105 5 0.12001455825260621 1105 6 -0.01902783851731016 1105 7 -0.014685151145719999 1105 8 0.037470814391291185 1105 9 0.06901481875206628 1105 10-11 0.06554836849606005 1105 12-13 0.007307925799899806 1105 14-15 -0.0057914914688481645 1105 16-17 -0.002569451147643065 1105 18-19 0.062067484303319986 1105 20-21 -0.0225340047217486 1105 22-23 0.01861245600363759 1105 24-25 0.006996698149102087 1105 26-27 -0.12177159728678077 1105 28-29 0.16000454481438453 1105 30-31 -0.07878117878548352 1105 32-33 0.008403740640638091 1105 34-35 0.0011760238245699384 1105 36-37 -0.030762840272778647 1105 38-39 0.0823362940356418 1105 40-41 0.008616188545389036 1105 42-43 0.07333282952500042 1105 44-45 -0.08758660024536624 1105 46-47 -0.0577410411685122 1105 48-49 0.07204132036446964 1105 50-51 0.024190971834819663 1105 52-53 0.27741795101762534 1105 54-55 0.19949695243330012 1105 56-57 0.2524688230598642 1105 58-59 0.24147475607700386 1105 60-61 0.1249746602093964 1105 62-63 0.10731219435589878 1105 64-65 0.14659371970668644 1105 66-67 0.16732893204383004 1105 68-69 0.29518359748398026 1105 70-71 0.3791688206378794 1105 72-73 0.45016537642131027 1105 74-75 0.414355367512119 1105 76-77 0.5397121231310784 1105 78-79 0.3922463416626343 1105 80-81 0.37584949078378216 1105 82-83 0.35596222586756454 1105 84-85 0.264514867885854 1105 86-87 0.29819390361007336 1105 88-89 0.3182013644055779 1105 90-91 0.23365157159791394 1105 92-93 0.4190914979069156 1105 94-95 0.5324768510085036 1105 96-97 0.5478079194544243 1105 98-99 0.5230217662139331 1105 100-101 0.9486111662432286 1106 1 0.17959780609244547 1106 2 0.1054652677275314 1106 3 0.06243851966932823 1106 4 0.10623083482206397 1106 5 0.03576432834421439 1106 6 0.06654137883867861 1106 7 0.06411744413190235 1106 8 0.1017346895122202 1106 9 0.2213813823083015 1106 10-11 0.13025377223907952 1106 12-13 0.13554007953397473 1106 14-15 0.08148076485810662 1106 16-17 0.21991170830725792 1106 18-19 -9.325152297634531E-5 1106 20-21 0.09049359025728876 1106 22-23 0.1588289580519131 1106 24-25 0.056885326939600134 1106 26-27 0.11349261821800383 1106 28-29 0.05366071531683758 1106 30-31 0.10251278746276427 1106 32-33 -0.01264332787803113 1106 34-35 -0.04451117833955465 1106 36-37 -0.2028037400804905 1106 38-39 0.0281820434175728 1106 40-41 0.03523181753892857 1106 42-43 -0.24260982475249193 1106 44-45 -0.313155474231678 1106 46-47 -0.3122440359492913 1106 48-49 -0.29487008428623795 1106 50-51 -0.37840624657219024 1106 52-53 -0.41176008168311284 1106 54-55 -0.27100796521769865 1106 56-57 -0.2633187176966203 1106 58-59 -0.20626400528404787 1106 60-61 -0.205485813461614 1106 62-63 -0.14159051557733449 1106 64-65 -0.1796186738487222 1106 66-67 -0.11572973042675727 1106 68-69 -0.09997705473926288 1106 70-71 -0.07553250745633022 1106 72-73 -0.16169326873150425 1106 74-75 -0.01838709626984425 1106 76-77 -0.020557093627751044 1106 78-79 -0.09583387845226454 1106 80-81 -0.1423046881745229 1106 82-83 -0.18153337004305214 1106 84-85 0.060608888476092915 1106 86-87 0.17410196712450698 1106 88-89 0.22995087771637657 1106 90-91 0.2678552973082411 1106 92-93 0.1769352557446986 1106 94-95 0.2227924823907017 1106 96-97 0.23861770459458143 1106 98-99 0.29231391708161425 1106 100-101 0.5966411946682619 1107 1 -0.038327756560121884 1107 2 -0.004226806834324748 1107 3 -0.10791764202539866 1107 4 -0.0868354047054467 1107 5 -0.12727912603385505 1107 6 -0.05100391863898324 1107 7 0.012872830062242713 1107 8 -0.0555260536015183 1107 9 -0.04454899664587231 1107 10-11 -0.05813642143102271 1107 12-13 -0.05463187473753095 1107 14-15 -0.06371615347057258 1107 16-17 -0.08281511232578254 1107 18-19 -0.02071560616419532 1107 20-21 -0.04827500095252191 1107 22-23 -0.04566244826273902 1107 24-25 -0.007110108706449125 1107 26-27 -0.15183187790501051 1107 28-29 -0.00855387523526474 1107 30-31 -0.06638277364442757 1107 32-33 -0.15548409623047377 1107 34-35 -0.04186371389236854 1107 36-37 -0.12714382399956747 1107 38-39 -0.1620029110597372 1107 40-41 -0.11532503071315148 1107 42-43 -0.1959998756034409 1107 44-45 -0.19088103146443558 1107 46-47 -0.3459193296257723 1107 48-49 -0.375272878268504 1107 50-51 -0.3363952575531499 1107 52-53 -0.609553635485284 1107 54-55 -0.6634069096742223 1107 56-57 -0.49904833685284444 1107 58-59 -0.28934429235788883 1107 60-61 -0.3583501066935284 1107 62-63 -0.5207431658249249 1107 64-65 -0.5636899794282613 1107 66-67 -0.42495500604943004 1107 68-69 -0.4242986820281267 1107 70-71 -0.5151518614303257 1107 72-73 -0.4959243486714193 1107 74-75 -0.454794850105273 1107 76-77 -0.5095363980325125 1107 78-79 -0.35463611639191583 1107 80-81 -0.1920963919192431 1107 82-83 -0.31716927785657845 1107 84-85 -0.25967571715625226 1107 86-87 -0.281483104391274 1107 88-89 -0.10110210965767408 1107 90-91 0.11411248312622391 1107 92-93 0.277111179656508 1107 94-95 0.22385428793252515 1107 96-97 0.22998245521401373 1107 98-99 0.3832232576745547 1107 100-101 0.9914010497397747 1108 1 0.20002666775202016 1108 2 0.0843245465034812 1108 3 0.15056228958869866 1108 4 0.07547405747459379 1108 5 0.009299341994136512 1108 6 0.08924469926277823 1108 7 0.07558973160571725 1108 8 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#Quality Count 2 2.0 3 6.0 4 28.0 5 78.0 6 239.0 7 483.0 8 883.0 9 1676.0 10 2877.0 11 4799.0 12 7014.0 13 9611.0 14 11850.0 15 16196.0 16 21671.0 17 30836.0 18 27078.0 19 14932.0 20 11826.0 21 11362.0 22 12048.0 23 13186.0 24 14738.0 25 16875.0 26 19243.0 27 22745.0 28 27233.0 29 32993.0 30 39765.0 31 47305.0 32 57512.0 33 71620.0 34 94205.0 35 133243.0 36 181366.0 37 305015.0 38 324568.0 39 39169.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08178538101877 23.437101301352385 16.514552539159528 18.96656077846932 2 14.17107038280048 24.79311655202862 40.65399642619528 20.381816638975625 3 18.0087906249731 30.56396261893723 32.973636733694974 18.453610022394695 4 10.605259371398985 20.677338068891054 41.56810828160991 27.149294278100054 5 9.671820288132892 41.86451738693739 36.033956454697154 12.429705870232562 6 23.949728911717518 42.270832475004966 19.11250794017795 14.666930673099568 7 22.56772827277993 37.62683870777321 23.96853428503614 15.836898734410724 8 23.814009658865213 35.04357803524367 21.882605556983492 19.25980674890763 9 25.229204354974982 18.535084407462932 24.017111465567385 32.2185997719947 10-11 21.95900700864182 29.393928959255426 29.129951951634347 19.517112080468408 12-13 24.8255218357501 28.475328326399303 29.086017273799435 17.613132564051163 14-15 21.60531594229277 29.22938144646856 29.978423123229852 19.186879488008817 16-17 17.15461000147273 32.46360217693617 31.009195805883603 19.37259201570749 18-19 16.92671855611402 30.92266512859425 34.699817620357656 17.45079869493408 20-21 18.7984772591156 28.869879565486343 34.35329015088441 17.978353024513645 22-23 18.214290545650865 29.10784625998753 34.35116874236754 18.326694451994065 24-25 18.419291255667492 29.934565006252033 34.38551581671069 17.260627921369785 26-27 17.939515579828115 29.16288881161516 35.68075333888597 17.21684226967075 28-29 16.862184694129787 30.176329016318242 35.41608507278583 17.545401216766138 30-31 17.805565838067047 29.710418513931813 34.86147509835342 17.622540549647724 32-33 17.558037432714457 29.995917057231296 34.97726096030322 17.468784549751028 34-35 18.180132674317605 29.613983842328178 34.80706878925076 17.39881469410345 36-37 18.226146243242223 29.171741759591736 34.95268901255322 17.64942298461282 38-39 17.493581050954923 30.35165078457238 34.76524327432512 17.389524890147566 40-41 17.42867614638109 30.170483581046888 34.939848315549895 17.460991957022124 42-43 17.15507892480121 30.3224852280236 33.851524764372485 18.670911082802704 44-45 17.740281630033977 29.853404677066912 33.657491660832555 18.748822032066556 46-47 18.616096958069694 30.52343706764559 32.74324066729387 18.117225306990843 48-49 18.294087480107535 30.488659758314252 32.55271041737895 18.664542344199273 50-51 17.455902373394956 30.827908881507344 32.94061654895412 18.775572196143585 52-53 17.743276364803556 31.683574395029634 31.952439865754812 18.620709374412 54-55 17.59284083041153 30.703422170133273 32.12061529455604 19.583121704899163 56-57 18.344626659218065 30.838881098326798 31.384459576001433 19.4320326664537 58-59 18.50569662654314 30.34444782983386 31.889978874965063 19.25987666865793 60-61 18.085751066653835 31.178759714566905 32.0367869130483 18.69870230573095 62-63 17.52664008832528 30.953140070031786 32.311992874454695 19.208226967188242 64-65 17.396152799619983 31.356358733833567 31.777973524940084 19.469514941606366 66-67 17.71678237822614 30.73879114729702 30.941721960188662 20.602704514288174 68-69 17.405415011171232 30.633647251034652 31.53054325666223 20.430394481131884 70-71 18.23023329673644 30.55848744048137 31.137478774365864 20.073800488416328 72-73 17.996338879330594 29.769203051384817 31.6988546853613 20.53560338392329 74-75 17.421419923247726 31.4685559786776 30.53596233346103 20.574061764613646 76-77 17.51966620593504 30.36281923456613 31.271936152625322 20.845578406873503 78-79 17.48171848545838 30.103668372048485 31.380657293994098 21.03395584849904 80-81 17.917441194791238 30.44668776621682 30.558262859728984 21.077608179262956 82-83 17.69783571025807 30.19364665327398 30.952227225519152 21.1562904109488 84-85 17.32874084258657 30.114992639634796 30.646236375330666 21.91003014244797 86-87 17.294130247577378 30.4589239207846 30.681411783823908 21.56553404781412 88-89 17.6854838908731 29.74391140905804 30.37563280125783 22.194971898811037 90-91 17.803310423500623 29.23414202943998 30.642004130930495 22.320543416128903 92-93 18.104678611770595 29.98644753986569 30.37506617892568 21.533807669438033 94-95 17.69696723735806 29.312790656919223 30.488362040075685 22.501880065647033 96-97 17.451326726219847 29.222841735189718 30.56987506744698 22.755956471143453 98-99 17.7500095309658 29.411730343766564 29.67011175211126 23.16814837315637 100-101 17.72334892020748 28.624025102973903 30.510068645171764 23.14255733164685 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8419.0 1 4977.5 2 1628.0 3 1630.5 4 1276.5 5 1325.5 6 2489.5 7 4627.0 8 6166.0 9 6270.0 10 5332.0 11 4424.0 12 4426.5 13 4673.0 14 4896.0 15 4981.5 16 5117.0 17 5488.5 18 6057.0 19 6692.0 20 7446.5 21 8323.5 22 9300.5 23 10456.5 24 11515.5 25 13534.0 26 17325.0 27 21310.0 28 23623.5 29 26847.5 30 32093.0 31 37220.5 32 45005.0 33 54702.0 34 63074.5 35 69306.5 36 77317.5 37 83895.5 38 80413.5 39 73792.5 40 68495.5 41 62704.0 42 56767.0 43 54781.0 44 53821.0 45 51106.5 46 48641.5 47 53541.0 48 73802.0 49 69438.5 50 45687.5 51 33988.5 52 24743.0 53 18250.0 54 13538.5 55 10609.5 56 8298.5 57 6268.5 58 4759.5 59 3607.5 60 2686.5 61 2055.0 62 1506.0 63 1067.5 64 802.5 65 597.5 66 435.5 67 323.5 68 191.5 69 114.0 70 83.5 71 65.5 72 44.0 73 34.0 74 34.5 75 44.5 76 35.0 77 24.5 78 27.5 79 25.0 80 19.0 81 12.5 82 5.5 83 1.0 84 0.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23440026858876525 2 0.0 3 0.0 4 0.0 5 0.02650223393540342 6 0.003381955606606005 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0029207798420688223 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.6117576453718245E-4 26-27 0.0015065074974881293 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00710210677387261 36-37 0.017309463695628913 38-39 0.014327193751621801 40-41 0.008547124169422448 42-43 0.008700849424268174 44-45 0.0040890917788963515 46-47 4.919208155063279E-4 48-49 0.002090663465901894 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013835272936115472 58-59 5.226658664754735E-4 60-61 0.0029207798420688223 62-63 0.0010145866819818014 64-65 0.0018754481091178754 66-67 0.006640931009335427 68-69 1.5372525484572748E-4 70-71 9.22351529074365E-5 72-73 0.0 74-75 4.3043071356803694E-4 76-77 0.05930720331948166 78-79 0.14151946961097672 80-81 0.001168311936827529 82-83 0.001045331732950947 84-85 0.0 86-87 0.10422572278540324 88-89 0.3508625216598884 90-91 0.38763360261898644 92-93 3.0745050969145497E-4 94-95 3.0745050969145497E-4 96-97 3.0745050969145494E-5 98-99 0.0 100-101 0.0010145866819818014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1626278.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.084460042280586 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20640147309636 20.4191527835658 2 12.809383954464915 7.451080315797297 3 5.498995394091991 4.798059354364605 4 2.9511760770741997 3.433334507655957 5 1.7947820058990354 2.610013276758735 6 1.2322211056010863 2.150309130546576 7 0.8665034360646381 1.764124919390456 8 0.6488891981972006 1.5098073565467174 9 0.4948572688259778 1.295339081562113 >10 2.834577367721746 15.51173548736734 >50 0.3272222722645486 6.778346470095226 >100 0.29793398436848095 17.056313412640556 >500 0.0251983943842862 4.8237780444956275 >1k 0.010164058239039812 5.497351749859798 >5k 0.001058756066566647 2.2441576528997915 >10k+ 6.352536399399883E-4 2.6570964564534867 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15780 0.970313808586232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13753 0.8456733719573161 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13611 0.8369417774820788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9438 0.5803435820935904 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9050 0.5564854225415335 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7476 0.4597000020906634 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5398 0.33192357026289476 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5077 0.31218524754070337 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4346 0.26723598302381263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3658 0.22493079289026846 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3652 0.2245618522786387 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3544 0.2179209212693033 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3483 0.21417002505106755 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3205 0.19707577671222265 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.1959074647753951 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3151 0.19375531120755493 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3011 0.18514669693619418 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2946 0.18114984031020528 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2815 0.17309463695628916 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2621 0.1611655571802607 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2258 0.13884465017666106 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1984 0.12199636224556933 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1965 0.1208280503087418 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1900 0.1168311936827529 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1847 0.11357221828002347 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1775 0.10914493094046651 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.149010193829099E-5 0.0 16-17 0.0 0.0 0.0 9.223515290743648E-5 0.0 18-19 0.0 0.0 0.0 1.5372525484572748E-4 0.0 20-21 0.0 0.0 0.0 6.149010193829099E-4 0.0 22-23 0.0 0.0 0.0 0.0027977996381922405 0.0 24-25 0.0 0.0 0.0 0.006886891417088591 0.0 26-27 0.0 0.0 0.0 0.012728451101226234 0.0 28-29 0.0 0.0 0.0 0.03025313015363917 0.0 30-31 0.0 0.0 0.0 0.07520239467052989 0.0 32-33 0.0 0.0 0.0 0.15633858417810487 0.0 34-35 0.0 0.0 0.0 0.2764594983145563 0.0 36-37 0.0 0.0 0.0 0.44325139982217066 0.0 38-39 0.0 0.0 0.0 0.6766063366779849 0.0 40-41 0.0 0.0 0.0 0.9437500845488902 0.0 42-43 0.0 0.0 0.0 1.1986880471850445 0.0 44-45 0.0 0.0 0.0 1.4951625736805148 0.0 46-47 0.0 0.0 0.0 1.8507598331896515 0.0 48-49 0.0 0.0 0.0 2.2241277321589545 0.0 50-51 0.0 0.0 0.0 2.6279639766386804 0.0 52-53 0.0 0.0 0.0 3.0009936800473227 0.0 54-55 0.0 0.0 0.0 3.396006094898904 0.0 56-57 0.0 0.0 0.0 3.8664976098797377 0.0 58-59 0.0 0.0 0.0 4.365920217822538 0.0 60-61 0.0 0.0 0.0 4.9125979691049135 0.0 62-63 0.0 0.0 0.0 5.448576442650026 0.0 64-65 0.0 0.0 0.0 5.990765416490907 0.0 66-67 0.0 0.0 0.0 6.592968729823561 0.0 68-69 0.0 0.0 0.0 7.258014927337147 0.0 70-71 0.0 0.0 0.0 8.00585754711064 0.0 72-73 0.0 0.0 0.0 8.73343917829547 0.0 74-75 0.0 0.0 0.0 9.480113486132137 0.0 76-77 0.0 0.0 0.0 10.225312031522286 0.0 78-79 0.0 0.0 0.0 11.104343783781125 0.0 80-81 0.0 0.0 0.0 12.031061110093109 0.0 82-83 0.0 0.0 0.0 12.922698333249297 0.0 84-85 0.0 0.0 0.0 13.834842505401905 0.0 86-87 0.0 0.0 0.0 14.751290984690193 0.0 88-89 0.0 0.0 0.0 15.708322931257754 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAGCGA 55 0.0025947723 34.50509 6 ATGGGCG 140 1.7222192E-8 30.50754 5 CATATAG 715 0.0 29.867521 3 GTAAGCG 110 1.8189894E-12 28.033663 94-95 ACCGCGA 35 0.008443057 27.110308 10-11 CATTCCG 390 0.0 26.761919 9 CGCGCCA 45 9.01562E-4 26.356436 94-95 GACATAT 740 0.0 25.072374 1 GAGTACT 12855 0.0 23.45395 12-13 CTTTGCG 345 0.0 23.384203 2 CATGGGG 4095 0.0 23.17762 4 ATGGGGT 1080 0.0 21.970451 5 GTACTTT 13730 0.0 21.890142 14-15 GGATATG 870 0.0 21.812222 9 GTACATG 12500 0.0 21.38892 1 CCTTTAG 625 0.0 21.312805 1 AGTACTT 13475 0.0 21.230541 12-13 GTGTACG 115 0.0037505764 20.626776 9 TTAGGCA 630 0.0 20.33836 4 ATGGGGA 2090 0.0 20.208609 5 >>END_MODULE