FastQCFastQC Report
Thu 26 May 2016
SRR938737_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938737_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262115
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT357800.6799547330303499No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT205030.3896342060179225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184760.3511135731545206No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT149040.28323212244506246No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC139200.26453241709844805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134090.25482149287881395No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT109710.20849031235539323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91060.1730482895185681No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT87010.16535176445212618No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC82050.15592589671643436No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC79730.1515170230981269No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG71050.1350217545606662No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC66670.12669810522955124No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA65120.12375252156214755No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA54330.10324745848389859No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG265600.024.6114561
TACATGG269350.023.780962
ACATGGG275200.022.5007423
GAGTACT169250.021.03631612-13
CATGGGG114900.020.6579574
AGTACTT177250.020.47417812-13
GTACTTT184150.019.21879614-15
CATGGGA150150.018.7741954
AGAGTAC345700.018.43375810-11
TACCTGG39350.017.5783372
TACTTTT214550.016.837714-15
ACTTTTT221850.016.26354816-17
AGTACAT205500.016.1612832
ATGGGAG66950.015.7098525
TATCACG2751.15736475E-515.5052772
GAGTACA244800.015.3167361
ATGGGGA61450.015.1884695
GTATAGG14250.014.9692061
GTGTAGC25750.014.7269871
GTATCAA680700.014.1225581
Produced by FastQC (version 0.11.5)