##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5262115 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.604345020965905 33.0 31.0 34.0 30.0 34.0 2 31.662220609013676 33.0 31.0 34.0 30.0 34.0 3 31.67880386498585 33.0 31.0 34.0 28.0 34.0 4 35.215774265670746 37.0 35.0 37.0 33.0 37.0 5 35.256547604907915 37.0 35.0 37.0 33.0 37.0 6 35.34967156742108 37.0 35.0 37.0 33.0 37.0 7 35.26581821187868 37.0 35.0 37.0 32.0 37.0 8 35.23973136276953 37.0 35.0 37.0 32.0 37.0 9 36.80169209528868 39.0 37.0 39.0 32.0 39.0 10-11 36.69521941272663 39.0 37.0 39.0 32.0 39.0 12-13 36.58682782493351 39.0 37.0 39.0 32.0 39.0 14-15 37.72576197973629 40.0 37.0 41.0 32.0 41.0 16-17 37.68187962444759 40.0 37.0 41.0 32.0 41.0 18-19 37.637399695749714 40.0 37.0 41.0 32.0 41.0 20-21 37.52856598535 40.0 37.0 41.0 32.0 41.0 22-23 37.43868263996511 40.0 37.0 41.0 32.0 41.0 24-25 37.44214864175336 40.0 37.0 41.0 31.5 41.0 26-27 37.34772406152279 40.0 37.0 41.0 31.0 41.0 28-29 37.24456886632086 40.0 37.0 41.0 31.0 41.0 30-31 37.06450837733497 40.0 36.5 41.0 30.5 41.0 32-33 36.86710657216727 39.5 36.0 41.0 30.0 41.0 34-35 36.53517463985489 39.0 35.5 41.0 30.0 41.0 36-37 36.28464590758659 39.0 35.0 41.0 30.0 41.0 38-39 36.124079291311574 39.0 35.0 41.0 29.0 41.0 40-41 35.97860641586131 39.0 35.0 41.0 28.5 41.0 42-43 35.71769554637251 39.0 35.0 40.5 27.0 41.0 44-45 35.67605791587603 39.0 35.0 41.0 27.0 41.0 46-47 35.48602539093122 39.0 35.0 41.0 26.0 41.0 48-49 35.24886637787277 39.0 35.0 41.0 25.0 41.0 50-51 34.09532887441647 38.0 33.5 40.0 23.5 40.5 52-53 34.206401038365755 38.0 33.5 39.5 23.5 40.5 54-55 34.7456421039829 38.5 34.0 40.0 24.0 41.0 56-57 34.64824913556622 38.0 34.0 40.0 23.5 41.0 58-59 34.43392096143851 38.0 34.0 40.0 23.0 41.0 60-61 34.124514762600214 38.0 34.0 40.0 22.5 41.0 62-63 33.78995327924228 37.5 33.5 40.0 22.0 41.0 64-65 33.40820649111622 37.0 33.0 40.0 21.0 41.0 66-67 32.99435274599662 36.0 32.5 39.0 19.5 41.0 68-69 32.58774257119048 36.0 32.0 39.0 18.5 41.0 70-71 32.17100215407683 35.0 32.0 38.5 17.5 40.0 72-73 31.762206071132994 35.0 31.0 37.5 14.0 40.0 74-75 31.321077361479176 35.0 31.0 37.0 11.0 39.0 76-77 30.898708028995948 35.0 31.0 36.5 7.5 39.0 78-79 30.488358673271108 35.0 30.5 36.0 6.5 38.5 80-81 30.04784910630041 34.0 30.0 36.0 2.0 37.5 82-83 29.59853709012441 34.0 29.5 35.0 2.0 37.0 84-85 29.20504730512351 34.0 29.0 35.0 2.0 36.5 86-87 28.863562939996562 34.0 29.0 35.0 2.0 36.0 88-89 28.50042910502716 34.0 29.0 35.0 2.0 36.0 90-91 28.097570083512046 34.0 28.5 35.0 2.0 35.0 92-93 27.67554794982626 34.0 27.0 35.0 2.0 35.0 94-95 27.187686415063144 34.0 26.0 35.0 2.0 35.0 96-97 26.62379965090083 33.0 25.0 35.0 2.0 35.0 98-99 25.97328070557181 33.0 24.5 35.0 2.0 35.0 100-101 23.805312122597094 31.0 10.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29603062131920055 1101 2 0.1314422194543461 1101 3 0.3049220300434037 1101 4 0.24615401316900432 1101 5 0.13748611750742157 1101 6 0.04158736708257038 1101 7 0.20994936433097422 1101 8 0.3086469636036071 1101 9 0.43342272729746156 1101 10-11 0.3496053903469516 1101 12-13 0.3484885402792415 1101 14-15 0.5264954522459888 1101 16-17 0.5855773273875826 1101 18-19 0.5893053583427204 1101 20-21 0.638335105344332 1101 22-23 0.5340590310744204 1101 24-25 0.5256126378046275 1101 26-27 0.6121725650722922 1101 28-29 0.5464263139433001 1101 30-31 0.47666969218420263 1101 32-33 0.41036665260644156 1101 34-35 0.41632881390903975 1101 36-37 0.48480108945829414 1101 38-39 0.49173580106023707 1101 40-41 0.519072289476334 1101 42-43 0.4360740497946338 1101 44-45 0.6436177728681471 1101 46-47 0.5557928707571946 1101 48-49 0.5586585494937637 1101 50-51 0.44388129701941637 1101 52-53 0.4354213902357813 1101 54-55 0.39964290683538195 1101 56-57 0.39994558274976555 1101 58-59 0.4772314462710554 1101 60-61 0.3566807091754782 1101 62-63 0.32254821215018126 1101 64-65 0.23477215405648622 1101 66-67 0.17601465421535067 1101 68-69 0.11354396371736186 1101 70-71 0.008117268677949596 1101 72-73 0.044039778655758255 1101 74-75 -0.005174378389497747 1101 76-77 0.012949992775297403 1101 78-79 -0.12114853857075047 1101 80-81 -0.159776458378591 1101 82-83 -0.25114793932918644 1101 84-85 -0.18231495482977067 1101 86-87 -0.13598994696939215 1101 88-89 -0.2042045841967628 1101 90-91 -0.24546965993853576 1101 92-93 -0.24841113206558063 1101 94-95 -0.4145074467016947 1101 96-97 -0.597464056984812 1101 98-99 -0.6343208842482042 1101 100-101 -0.5574570489010817 1102 1 0.32498047838854305 1102 2 0.3013157459895872 1102 3 0.36402718919273624 1102 4 0.3114129484944854 1102 5 0.2976622779384286 1102 6 0.26999439353056687 1102 7 0.2640909776525575 1102 8 0.25499515146601226 1102 9 0.4540210026838025 1102 10-11 0.39461083672057384 1102 12-13 0.40425435841680013 1102 14-15 0.2877162260288486 1102 16-17 0.3595011158765118 1102 18-19 0.5228510418717249 1102 20-21 0.4270064580213102 1102 22-23 0.35203311004454463 1102 24-25 0.3259132620970888 1102 26-27 0.42681154253288156 1102 28-29 0.4447306002147755 1102 30-31 0.475247002399918 1102 32-33 0.4929275908946593 1102 34-35 0.44961651382138257 1102 36-37 0.44812313488578326 1102 38-39 0.3963911786588312 1102 40-41 0.4313130874980473 1102 42-43 0.3956557844658235 1102 44-45 0.4167538063708278 1102 46-47 0.3760470095019173 1102 48-49 0.45276699643651597 1102 50-51 0.42374223500652164 1102 52-53 0.4055372181915544 1102 54-55 0.38179864380113315 1102 56-57 0.40967119899509186 1102 58-59 0.42718745053940665 1102 60-61 0.39053884005738837 1102 62-63 0.31739567168988003 1102 64-65 0.36373836688773764 1102 66-67 0.30085979351905223 1102 68-69 0.2581449457287164 1102 70-71 0.25424731632045194 1102 72-73 0.17404233186626428 1102 74-75 0.054098775988766334 1102 76-77 0.12411564510473383 1102 78-79 -0.017311717725139175 1102 80-81 -0.09176018249365825 1102 82-83 -0.1564066337558465 1102 84-85 -0.15674544704152638 1102 86-87 -0.14282937348439972 1102 88-89 -0.129791731484044 1102 90-91 -0.13325062997785508 1102 92-93 -0.29915060427211415 1102 94-95 -0.3722376282966131 1102 96-97 -0.514002046173843 1102 98-99 -0.6292519451872067 1102 100-101 -0.578831192818793 1103 1 0.1410496204287206 1103 2 0.2470270363297402 1103 3 0.15853025889698102 1103 4 0.2724690371102625 1103 5 0.2016869446742291 1103 6 0.23352902211912152 1103 7 0.20366368247147904 1103 8 0.21825632004239282 1103 9 0.3289607399819232 1103 10-11 0.286883502609264 1103 12-13 0.3689313820777116 1103 14-15 0.4719016941490466 1103 16-17 0.4880511288441127 1103 18-19 0.48752244192819916 1103 20-21 0.41948199690957466 1103 22-23 0.4757715905709148 1103 24-25 0.37371350790542834 1103 26-27 0.416248258015564 1103 28-29 0.37083054001281823 1103 30-31 0.372691738435762 1103 32-33 0.2917727102447927 1103 34-35 0.35976158243758505 1103 36-37 0.36116698544102377 1103 38-39 0.2626232053643278 1103 40-41 0.45590083810407833 1103 42-43 0.33591701833088194 1103 44-45 0.3651767909317485 1103 46-47 0.43005998619448604 1103 48-49 0.3891532141459493 1103 50-51 0.3553782508736205 1103 52-53 0.34834639553778146 1103 54-55 0.3417854637966471 1103 56-57 0.3332234892285726 1103 58-59 0.34102211312039543 1103 60-61 0.2818378581956438 1103 62-63 0.26958619690004326 1103 64-65 0.18098176456059178 1103 66-67 -0.0032584836969817843 1103 68-69 -0.06372597299424854 1103 70-71 0.031007914032393558 1103 72-73 0.1023194883304619 1103 74-75 -0.07084962205206935 1103 76-77 -0.12508666522088774 1103 78-79 0.024060375919667365 1103 80-81 -0.02001929247811418 1103 82-83 -0.03838720218429614 1103 84-85 0.016063312551221287 1103 86-87 0.003638305368365735 1103 88-89 -0.1100095957981857 1103 90-91 -0.04262998704663801 1103 92-93 -0.08232242455096639 1103 94-95 -0.19875960099277634 1103 96-97 -0.1537947808844784 1103 98-99 -0.06705825038033453 1103 100-101 0.03007739214046623 1104 1 0.08971416058139425 1104 2 0.07005703136100294 1104 3 0.1153673278440479 1104 4 0.22706373032227134 1104 5 0.11389236805029412 1104 6 0.06555429317780437 1104 7 0.0826000334115804 1104 8 0.12315464365146056 1104 9 0.1650733493164367 1104 10-11 0.10515443851753048 1104 12-13 0.16883758756544154 1104 14-15 0.19757637109120907 1104 16-17 0.254712492502847 1104 18-19 0.14874704313653808 1104 20-21 0.1698759932173104 1104 22-23 0.2705511580422808 1104 24-25 0.14250583727236688 1104 26-27 0.16314955193388414 1104 28-29 0.2818885728827212 1104 30-31 0.18203351019023728 1104 32-33 0.16808432608146262 1104 34-35 0.11410339089918864 1104 36-37 0.1556061529109556 1104 38-39 0.28540544292752656 1104 40-41 0.17236837252716697 1104 42-43 0.2762532977623806 1104 44-45 0.3199204799227502 1104 46-47 0.34139373252201466 1104 48-49 0.4512755505559696 1104 50-51 0.4177008949361962 1104 52-53 0.4419378471202222 1104 54-55 0.4152832403602247 1104 56-57 0.35213578060128725 1104 58-59 0.3538557437814376 1104 60-61 0.35353962155903673 1104 62-63 0.2127956679003944 1104 64-65 0.08093979738363544 1104 66-67 0.10412824709937496 1104 68-69 0.11836055600664963 1104 70-71 0.10729693546263519 1104 72-73 0.02087297927949905 1104 74-75 0.025140932970582952 1104 76-77 -0.054933727721561354 1104 78-79 -0.039623380022000276 1104 80-81 -0.07761817873710797 1104 82-83 -0.1077946921870705 1104 84-85 -0.05479869724661057 1104 86-87 0.009669754470341019 1104 88-89 -0.016773656618298105 1104 90-91 -0.08951783209946385 1104 92-93 -0.11833617457107692 1104 94-95 -0.05724711596264598 1104 96-97 -0.040929336080822054 1104 98-99 -0.06913673271100862 1104 100-101 0.06231950467763525 1105 1 0.1121888609936974 1105 2 0.1385746250136215 1105 3 0.17732733324227468 1105 4 0.22943891856393606 1105 5 0.15697174612829912 1105 6 0.2015343938038896 1105 7 0.2123688094559455 1105 8 0.22481814758277352 1105 9 0.1334934291207972 1105 10-11 0.2596728428219137 1105 12-13 0.30088695286091394 1105 14-15 0.39433710733070626 1105 16-17 0.2637682897646201 1105 18-19 0.356759168332502 1105 20-21 0.27921379722319273 1105 22-23 0.400983771887752 1105 24-25 0.3910663107003387 1105 26-27 0.327717215038156 1105 28-29 0.4331748513852105 1105 30-31 0.2957340375576152 1105 32-33 0.3900359357714578 1105 34-35 0.46584558068717996 1105 36-37 0.40005621795588553 1105 38-39 0.41687485864927254 1105 40-41 0.3085647540641361 1105 42-43 0.37762118462979544 1105 44-45 0.450551204634408 1105 46-47 0.42285681359239646 1105 48-49 0.26475897075359 1105 50-51 0.34624021307677566 1105 52-53 0.2520368574150993 1105 54-55 0.4229943778096157 1105 56-57 0.3144989238246936 1105 58-59 0.3758866360706392 1105 60-61 0.3961688798141978 1105 62-63 0.3225194036554697 1105 64-65 0.30303087651139293 1105 66-67 0.24407339575100195 1105 68-69 0.1690480569438364 1105 70-71 0.2229983864038232 1105 72-73 0.1988036965548261 1105 74-75 0.12809887088549132 1105 76-77 0.21954097819913443 1105 78-79 0.13978377770561323 1105 80-81 0.22031298379994624 1105 82-83 0.19019300787850568 1105 84-85 0.10218528649016534 1105 86-87 0.15798551756172685 1105 88-89 0.13135153139385025 1105 90-91 0.28447111010862614 1105 92-93 0.3001168943839012 1105 94-95 0.2870961168700674 1105 96-97 0.16955953834343518 1105 98-99 0.2109048923749448 1105 100-101 0.25692630644069325 1106 1 0.04250233100954759 1106 2 0.09282572540703171 1106 3 0.1537093088300452 1106 4 0.1434377811092702 1106 5 0.05882436935280566 1106 6 0.032912802719451406 1106 7 0.0018995275530286904 1106 8 0.1096295656631483 1106 9 0.1509482196936318 1106 10-11 0.15256357684093302 1106 12-13 0.16188962127136364 1106 14-15 0.196849686178723 1106 16-17 0.19386075646178824 1106 18-19 0.35778271079439605 1106 20-21 0.2782168949075654 1106 22-23 0.24747369956297405 1106 24-25 0.2017981136235818 1106 26-27 0.07090525763691602 1106 28-29 0.14733013668412553 1106 30-31 0.16788331453722094 1106 32-33 0.08817734788262754 1106 34-35 0.11750194599202501 1106 36-37 0.2831634245739778 1106 38-39 0.21561264624538268 1106 40-41 0.26485918778178785 1106 42-43 0.3349929716311024 1106 44-45 0.27140391365306726 1106 46-47 0.31957455809740765 1106 48-49 0.35339653531521265 1106 50-51 0.317912415436993 1106 52-53 0.23436735408444065 1106 54-55 0.16976906325883334 1106 56-57 0.282130880843944 1106 58-59 0.22229641593738592 1106 60-61 0.22915638388032988 1106 62-63 0.11681431212098659 1106 64-65 0.12372620782385724 1106 66-67 -0.056084542800377335 1106 68-69 0.17134953063276726 1106 70-71 0.17315137587539908 1106 72-73 0.09559059933428316 1106 74-75 0.07943403105832658 1106 76-77 -0.05961111028222277 1106 78-79 0.24101788721836925 1106 80-81 0.13484915876909298 1106 82-83 0.21174575779339477 1106 84-85 0.26377092315404127 1106 86-87 0.3880787345788015 1106 88-89 0.4284001897157488 1106 90-91 0.35510433160639465 1106 92-93 0.4902990038469319 1106 94-95 0.5665146068141134 1106 96-97 0.5716772367075542 1106 98-99 0.7207339694996477 1106 100-101 0.8042365914622103 1107 1 0.0874545572297265 1107 2 0.1755476504519038 1107 3 0.11145362598056252 1107 4 0.14802326912667496 1107 5 0.07228617702152462 1107 6 0.10579095051565446 1107 7 0.048073384013399334 1107 8 0.15284039786649117 1107 9 0.14335091932147748 1107 10-11 0.08046930103424899 1107 12-13 0.14497201472942578 1107 14-15 0.15184511285065838 1107 16-17 0.1759688708863365 1107 18-19 0.26612329561094583 1107 20-21 0.1337464400304853 1107 22-23 0.21956750083496956 1107 24-25 0.16020596373230944 1107 26-27 0.1582408461963638 1107 28-29 -0.023790927792099126 1107 30-31 0.1505439652903675 1107 32-33 0.23637846102237603 1107 34-35 0.2413544617969947 1107 36-37 0.2966485882469314 1107 38-39 0.36501136630669606 1107 40-41 0.3245437141546432 1107 42-43 0.38655479875573207 1107 44-45 0.4525634628925701 1107 46-47 0.40234838537389095 1107 48-49 0.41821730652898736 1107 50-51 0.5240012738370226 1107 52-53 0.42470633200790076 1107 54-55 0.4226702497141872 1107 56-57 0.3541271355789135 1107 58-59 0.317032966141376 1107 60-61 0.2684163440668641 1107 62-63 0.24849642785762427 1107 64-65 0.19100343751407678 1107 66-67 0.23246383779067514 1107 68-69 0.2744424142733095 1107 70-71 0.3487193494105938 1107 72-73 0.24877736669720818 1107 74-75 0.2849342440057683 1107 76-77 0.2895822845508036 1107 78-79 0.3857918960043776 1107 80-81 0.3684187658899596 1107 82-83 0.36429131015770366 1107 84-85 0.23010854450145857 1107 86-87 0.24962219127928975 1107 88-89 0.2818324398050187 1107 90-91 0.32357261370777834 1107 92-93 0.4699863240427753 1107 94-95 0.5026168797166406 1107 96-97 0.43095308490865136 1107 98-99 0.4737191634241782 1107 100-101 0.655598535678287 1108 1 0.17723298176569102 1108 2 0.11469227485016376 1108 3 0.09569189873287698 1108 4 0.1440988113980879 1108 5 0.16371725322314035 1108 6 0.16134486128590453 1108 7 0.17267656168139212 1108 8 0.203608913071605 1108 9 0.11837485452322483 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sequence quality scores pass #Quality Count 2 17801.0 3 5527.0 4 5487.0 5 6111.0 6 6968.0 7 8483.0 8 10404.0 9 12606.0 10 15260.0 11 19660.0 12 24747.0 13 31237.0 14 33611.0 15 32074.0 16 39779.0 17 49883.0 18 43093.0 19 31453.0 20 32311.0 21 34879.0 22 37967.0 23 42957.0 24 49526.0 25 57211.0 26 65913.0 27 77190.0 28 91093.0 29 108821.0 30 129677.0 31 156728.0 32 194600.0 33 245654.0 34 325753.0 35 453473.0 36 677306.0 37 1012537.0 38 920882.0 39 153413.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.210561580285415 18.89285151399097 14.325143541525968 22.57144336419765 2 14.993874123655393 21.01172732973992 40.07822024128021 23.916178305324483 3 20.07428502847409 25.07532447870097 29.85185028797431 24.998540204850627 4 12.599213992523437 16.549663448103207 38.20913642854928 32.64198613082407 5 13.437632716185629 37.960425270227276 33.98287837527228 14.619063638314811 6 30.863616635269786 36.22129485937183 17.85971954402949 15.055368961328893 7 27.941271530007977 33.22573874477459 21.124047219435926 17.708942505781515 8 25.87760541931019 33.7537430770898 20.17116913289896 20.197482370701046 9 26.334309757632944 17.66039561059664 21.156491466094717 34.8488031656757 10-11 25.003826222390124 26.247581399673024 27.407463880935946 21.34112849700091 12-13 26.64614769174841 24.02469273823743 27.52090399089499 21.80825557911917 14-15 23.128287228752384 25.247894134491155 27.038154312227164 24.5856643245293 16-17 21.847068316339996 28.57189980888673 27.22031337100843 22.360718503764847 18-19 21.591720613780243 27.83947704256245 29.191048074787734 21.377754268869577 20-21 23.10598980907076 26.143950725779053 28.703557052224564 22.046502412925623 22-23 22.39910684632301 26.3424046709914 28.82481015397526 22.433678328710332 24-25 22.828629769260292 26.383196273108112 28.881329517785627 21.906844439845973 26-27 22.441961313882498 26.11895886033817 29.595051067957474 21.844028757821864 28-29 21.680816532388427 27.38248150140085 29.070566631090735 21.866135335119992 30-31 22.139577646507373 26.359898951579858 28.92646987515367 22.5740535267591 32-33 22.103640610031363 26.577515130191276 29.049206716045234 22.269637543732127 34-35 22.912030713869303 26.416317829534936 28.721087451212597 21.95056400538316 36-37 22.6701292710076 26.0306159985343 29.265269690841215 22.03398503961688 38-39 22.221641030712174 26.795040204983277 29.11275664669383 21.87056211761072 40-41 22.359688748361283 26.799257732289313 28.812486175044405 22.028567344304996 42-43 22.03364930968371 26.92299872231707 28.140429620289055 22.90292234771017 44-45 22.407551824906292 26.421181951633365 28.256470076317676 22.914796147142667 46-47 22.87795478698116 26.96355411093575 28.04003728483606 22.118453817247026 48-49 22.767592056044116 26.877425030704206 27.96839703471325 22.38658587853843 50-51 21.880266972935146 27.169441508735048 28.49895030488416 22.451341213445644 52-53 22.520415324024345 27.55832438238453 27.44738035565103 22.47387993794009 54-55 21.98162469063577 26.780364665057967 27.95259781943325 23.285412824873017 56-57 22.560708066299608 26.888145778580387 27.696565376394066 22.854580778725943 58-59 23.283038583483034 26.608045919426292 27.51205361083477 22.5968618862559 60-61 22.574687735035837 26.77876457055244 28.546548877341966 22.09999881706976 62-63 22.232542796044854 26.858397707528088 28.38204467059557 22.527014825831486 64-65 21.943962779739927 27.497867959288264 27.89345210693176 22.664717154040048 66-67 22.316544977802657 27.01517608670347 27.357587456082527 23.310691479411343 68-69 21.995083949892884 26.940357604540075 27.862484681512996 23.20207376405404 70-71 22.629465201481995 26.760410447290827 27.656430314149866 22.953694037077316 72-73 22.486724868537788 26.421963788437818 28.207313680979173 22.883997662045218 74-75 21.980082930341627 27.674714533682256 27.506531716840634 22.838670819135487 76-77 22.25009367474696 26.779502772056524 27.590017255246384 23.380386297950135 78-79 22.341024568674346 26.94673717246746 27.719398340149958 22.992839918708235 80-81 22.7068235673204 26.879330294545213 27.476608022198292 22.937238115936097 82-83 22.46793955781189 26.716242869173083 27.966176765660617 22.849640807354415 84-85 22.25329902698451 26.998466523389457 27.71977803009585 23.028456419530183 86-87 22.28459412002752 26.975657521479036 27.7127567104895 23.026991648003943 88-89 22.208377081421617 26.96087787125025 27.221051097926242 23.60969394940189 90-91 22.421634359239746 26.512573830315834 27.506736151027273 23.55905565941715 92-93 22.935788401166068 26.830150922278413 27.45118727059767 22.78287340595785 94-95 22.662009337066735 26.54320751884811 27.532802529403206 23.261980614681953 96-97 22.089959101686606 26.49471824412734 28.04765291845097 23.367669735735078 98-99 22.46154900853764 26.852060584342972 27.131734538993417 23.554655868125963 100-101 22.557976039727066 25.997635057212293 27.724037564135664 23.72035133892498 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3088.0 1 1809.0 2 714.5 3 926.5 4 842.0 5 857.5 6 1416.0 7 2902.0 8 4786.5 9 5656.0 10 5349.5 11 5192.5 12 5664.0 13 6303.0 14 7018.5 15 7581.0 16 8162.5 17 8666.0 18 8970.5 19 9345.5 20 10032.0 21 11066.0 22 12450.0 23 14384.5 24 16902.0 25 20775.5 26 26849.5 27 34250.5 28 40940.0 29 48376.0 30 57815.5 31 68485.0 32 78807.5 33 88141.0 34 99482.0 35 109095.0 36 119495.5 37 132038.5 38 138270.0 39 141326.0 40 145736.5 41 152829.0 42 159846.0 43 166847.5 44 174267.0 45 180369.0 46 187188.0 47 214203.0 48 273976.0 49 275552.5 50 220464.5 51 191479.5 52 178404.0 53 173549.5 54 168414.0 55 156521.0 56 142391.5 57 127581.5 58 115365.5 59 102518.0 60 87036.0 61 71419.5 62 56918.5 63 44749.5 64 33704.0 65 25247.0 66 18700.0 67 14487.0 68 10875.5 69 7370.5 70 5399.5 71 4025.5 72 3115.5 73 2486.0 74 1906.5 75 1477.5 76 1020.0 77 564.5 78 356.5 79 245.0 80 181.0 81 135.0 82 106.0 83 89.0 84 66.0 85 42.5 86 33.5 87 30.5 88 27.5 89 23.5 90 18.5 91 14.5 92 11.0 93 12.0 94 11.5 95 7.0 96 3.5 97 3.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16310932011178017 2 0.09327048154591833 3 0.08604905061938023 4 0.10303841706234089 5 0.12426562323324367 6 0.03872967428495957 7 0.042701461294555515 8 0.04570405625874767 9 0.04652121817938225 10-11 0.04481087927572849 12-13 0.049276764190824406 14-15 0.04675876524933416 16-17 0.045742063789939974 18-19 0.05022695247063205 20-21 0.05191828760868966 22-23 0.06547747436154475 24-25 0.08226730126574582 26-27 0.08390162510701495 28-29 0.07371560674747701 30-31 0.061762238187496855 32-33 0.05093959368048779 34-35 0.2994138288501867 36-37 0.47540770203615845 38-39 0.4529357492187077 40-41 0.42750871085105513 42-43 0.458323316765217 44-45 0.4674831317825627 46-47 0.4674356223685723 48-49 0.4837313513672734 50-51 0.47281368803228363 52-53 0.4796550436468986 54-55 0.47055223992634143 56-57 0.4489164527951213 58-59 0.4276322353274301 60-61 0.39704567460042206 62-63 0.45469359753635186 64-65 0.4928531588534268 66-67 0.4943924638667152 68-69 0.5057092062792242 70-71 0.5028586414398013 72-73 0.49247308354150376 74-75 0.4855747166301002 76-77 0.4670650489394474 78-79 0.441039391955516 80-81 0.4468450423451407 82-83 0.4583328186480151 84-85 0.45642294020560176 86-87 0.4679582259224665 88-89 0.4792654664521775 90-91 0.48355081559411 92-93 0.48546069403652337 94-95 0.4899360808344173 96-97 0.4909717860594077 98-99 0.48424445303836955 100-101 0.47089430770707214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5262115.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.26214020185223 #Duplication Level Percentage of deduplicated Percentage of total 1 71.06784919904513 31.456151050922283 2 14.446013749925601 12.788229719141844 3 5.625381713305201 7.469743022497516 4 2.802439401157818 4.961678627249687 5 1.5743143651302713 3.4841261575593037 6 0.9907220694699894 2.631088748396992 7 0.6865841429412315 2.127277851666334 8 0.47608542682849264 1.6858047928273125 9 0.33716842446397327 1.3431416467735813 >10 1.760660965911485 14.017739480001893 >50 0.13653670652881902 4.200094105925044 >100 0.08888601627192136 7.221370355126945 >500 0.005034298872518809 1.551688285783931 >1k 0.001678097884578518 1.4877523629076166 >5k 3.442252070927745E-4 1.1370353645208369 >10k+ 3.011970562061777E-4 2.4370784286988685 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35780 0.6799547330303499 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20503 0.3896342060179225 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18476 0.3511135731545206 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14904 0.28323212244506246 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13920 0.26453241709844805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13409 0.25482149287881395 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10971 0.20849031235539323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9106 0.1730482895185681 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8701 0.16535176445212618 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8205 0.15592589671643436 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7973 0.1515170230981269 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7105 0.1350217545606662 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6667 0.12669810522955124 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6512 0.12375252156214755 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5433 0.10324745848389859 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9003765596152878E-5 0.0 5 1.9003765596152878E-5 0.0 0.0 1.9003765596152878E-5 0.0 6 1.9003765596152878E-5 0.0 0.0 1.9003765596152878E-5 0.0 7 5.701129678845863E-5 0.0 0.0 1.9003765596152878E-5 0.0 8 5.701129678845863E-5 0.0 0.0 1.9003765596152878E-5 0.0 9 5.701129678845863E-5 0.0 0.0 1.9003765596152878E-5 0.0 10-11 5.701129678845863E-5 0.0 0.0 4.750941399038219E-5 0.0 12-13 5.701129678845863E-5 0.0 0.0 7.601506238461151E-5 0.0 14-15 5.701129678845863E-5 0.0 0.0 9.501882798076439E-5 9.501882798076439E-6 16-17 5.701129678845863E-5 0.0 0.0 1.235244763749937E-4 1.9003765596152878E-5 18-19 5.701129678845863E-5 0.0 0.0 1.5203012476922302E-4 1.9003765596152878E-5 20-21 5.701129678845863E-5 0.0 0.0 1.9953953875960522E-4 1.9003765596152878E-5 22-23 5.701129678845863E-5 0.0 0.0 5.511092022884334E-4 1.9003765596152878E-5 24-25 9.501882798076439E-5 0.0 0.0 0.0010547089905864847 1.9003765596152878E-5 26-27 9.501882798076439E-5 0.0 0.0 0.0018528671456249056 1.9003765596152878E-5 28-29 1.3302635917307015E-4 0.0 0.0 0.005188028007749736 1.9003765596152878E-5 30-31 1.5203012476922302E-4 0.0 0.0 0.015383548250085755 1.9003765596152878E-5 32-33 1.5203012476922302E-4 0.0 0.0 0.03744692010721924 1.9003765596152878E-5 34-35 1.8053577316345232E-4 0.0 0.0 0.06904068041082341 1.9003765596152878E-5 36-37 2.2804518715383452E-4 0.0 0.0 0.12027483245805157 1.9003765596152878E-5 38-39 3.0406024953844604E-4 0.0 0.0 0.19818076951948027 1.9003765596152878E-5 40-41 3.0406024953844604E-4 0.0 0.0 0.28668130590076424 1.9003765596152878E-5 42-43 3.1356213233652247E-4 0.0 0.0 0.37988527426709606 1.9003765596152878E-5 44-45 3.3256589793267537E-4 0.0 0.0 0.4942974450387344 1.9003765596152878E-5 46-47 4.370866087115162E-4 0.0 0.0 0.6303834104727852 1.9003765596152878E-5 48-49 4.940979054999748E-4 0.0 0.0 0.7710112758843164 3.8007531192305756E-5 50-51 4.940979054999748E-4 0.0 0.0 0.9274977836858374 5.701129678845864E-5 52-53 4.940979054999748E-4 0.0 0.0 1.0861032113513294 7.601506238461151E-5 54-55 4.940979054999748E-4 1.9003765596152878E-5 0.0 1.2591800065182914 7.601506238461151E-5 56-57 4.940979054999748E-4 1.9003765596152878E-5 0.0 1.4726873129910691 7.601506238461151E-5 58-59 4.940979054999748E-4 1.9003765596152878E-5 0.0 1.7007895114416922 7.601506238461151E-5 60-61 4.940979054999748E-4 1.9003765596152878E-5 0.0 1.9469642909742566 7.601506238461151E-5 62-63 5.321054366922806E-4 1.9003765596152878E-5 0.0 2.1991537623180033 7.601506238461151E-5 64-65 5.321054366922806E-4 1.9003765596152878E-5 0.0 2.4610351541157884 7.601506238461151E-5 66-67 5.701129678845863E-4 1.9003765596152878E-5 0.0 2.7602874509584074 7.601506238461151E-5 68-69 5.701129678845863E-4 1.9003765596152878E-5 0.0 3.1040655705928133 7.601506238461151E-5 70-71 5.891167334807392E-4 1.9003765596152878E-5 0.0 3.4729191589313424 7.601506238461151E-5 72-73 5.986186162788157E-4 1.9003765596152878E-5 0.0 3.841193132419189 7.601506238461151E-5 74-75 6.176223818749685E-4 1.9003765596152878E-5 0.0 4.231825036130909 7.601506238461151E-5 76-77 6.271242646730449E-4 1.9003765596152878E-5 0.0 4.64492889266008 7.601506238461151E-5 78-79 6.366261474711214E-4 1.9003765596152878E-5 0.0 5.125173053040459 7.601506238461151E-5 80-81 7.601506238461151E-4 1.9003765596152878E-5 0.0 5.641467356756742 7.601506238461151E-5 82-83 7.791543894422679E-4 3.8007531192305756E-5 0.0 6.152668651293254 7.601506238461151E-5 84-85 7.886562722403444E-4 3.8007531192305756E-5 0.0 6.678597864166784 7.601506238461151E-5 86-87 7.981581550384209E-4 3.8007531192305756E-5 0.0 7.221934526326391 7.601506238461151E-5 88-89 7.981581550384209E-4 3.8007531192305756E-5 0.0 7.805150590589525 7.601506238461151E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 26560 0.0 24.611456 1 TACATGG 26935 0.0 23.78096 2 ACATGGG 27520 0.0 22.500742 3 GAGTACT 16925 0.0 21.036316 12-13 CATGGGG 11490 0.0 20.657957 4 AGTACTT 17725 0.0 20.474178 12-13 GTACTTT 18415 0.0 19.218796 14-15 CATGGGA 15015 0.0 18.774195 4 AGAGTAC 34570 0.0 18.433758 10-11 TACCTGG 3935 0.0 17.578337 2 TACTTTT 21455 0.0 16.8377 14-15 ACTTTTT 22185 0.0 16.263548 16-17 AGTACAT 20550 0.0 16.161283 2 ATGGGAG 6695 0.0 15.709852 5 TATCACG 275 1.15736475E-5 15.505277 2 GAGTACA 24480 0.0 15.316736 1 ATGGGGA 6145 0.0 15.188469 5 GTATAGG 1425 0.0 14.969206 1 GTGTAGC 2575 0.0 14.726987 1 GTATCAA 68070 0.0 14.122558 1 >>END_MODULE