FastQCFastQC Report
Thu 26 May 2016
SRR938737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262115
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT389100.7394365193463085No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT187180.35571248442878955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180370.34277092005780946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144800.2751745258322937No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT138860.2638862890681789No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC129360.24583271175183363No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT111370.21164493744435459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98280.18676900827899048No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC93780.17821731376072167No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC93690.17804627987035632No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT88440.16806930293237604No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG78950.15003472938162696No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72440.13766327797853145No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC66860.12705917677587814No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA61210.11632204921405176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG256550.023.2099191
TACATGG260200.022.4860292
ACATGGG259450.021.6914223
GAGTACT177300.021.13114712-13
CATGGGG108600.020.405354
AGTACTT187700.020.30163212-13
GTACTTT195450.019.30248514-15
AGAGTAC356550.018.0673710-11
CATGGGA140950.017.3380054
CGCGGAA5550.017.2114582-83
ATGGGGA59500.016.5086045
ACTTTTT232200.016.37995516-17
TACTTTT230550.016.31232514-15
TCGCGCT6150.015.81757360-61
GGACCGT4802.1827873E-1115.8174216
ATTCGCG6400.015.23654580-81
AGTACAT201100.015.2196482
TATACCG3806.97255E-814.9849265
GAGTACA239850.014.9352931
GTACACG7050.014.8651461
Produced by FastQC (version 0.11.5)