##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938737_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5262115 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02240791012739 33.0 31.0 34.0 30.0 34.0 2 32.239377132578824 34.0 31.0 34.0 30.0 34.0 3 32.334134468744985 34.0 31.0 34.0 30.0 34.0 4 35.947006099258566 37.0 35.0 37.0 35.0 37.0 5 35.86141903018083 37.0 35.0 37.0 35.0 37.0 6 35.89463400172744 37.0 35.0 37.0 35.0 37.0 7 35.83071825682259 37.0 35.0 37.0 35.0 37.0 8 35.80183253311644 37.0 35.0 37.0 33.0 37.0 9 37.450854266772964 39.0 37.0 39.0 34.0 39.0 10-11 37.37579376353425 39.0 37.0 39.0 34.0 39.0 12-13 37.310322560415344 39.0 37.0 39.0 34.0 39.0 14-15 38.51931039135405 40.0 38.0 41.0 34.0 41.0 16-17 38.4728589169944 40.0 38.0 41.0 34.0 41.0 18-19 38.508734890818616 40.0 38.0 41.0 33.5 41.0 20-21 38.58223993584329 40.0 38.0 41.0 34.0 41.0 22-23 38.53790158519911 40.0 38.0 41.0 34.0 41.0 24-25 38.46288146496228 40.0 38.0 41.0 34.0 41.0 26-27 38.30143088853056 40.0 38.0 41.0 33.5 41.0 28-29 38.16046532620439 40.0 38.0 41.0 33.0 41.0 30-31 37.95980950625366 40.0 37.0 41.0 33.0 41.0 32-33 37.74950233888845 40.0 37.0 41.0 33.0 41.0 34-35 37.71058595260651 40.0 37.0 41.0 33.0 41.0 36-37 37.63264124786326 40.0 37.0 41.0 32.5 41.0 38-39 37.53718077237005 40.0 37.0 41.0 32.0 41.0 40-41 37.41281670963102 40.0 37.0 41.0 32.0 41.0 42-43 37.234772235118385 40.0 36.0 41.0 31.0 41.0 44-45 36.98451202605796 40.0 36.0 41.0 31.0 41.0 46-47 36.69689535481456 40.0 36.0 41.0 30.0 41.0 48-49 36.41801728012406 39.0 36.0 41.0 30.0 41.0 50-51 36.1865102720104 39.0 35.0 41.0 29.5 41.0 52-53 35.99811672682942 39.0 35.0 41.0 29.0 41.0 54-55 35.77379703788306 39.0 35.0 40.5 28.0 41.0 56-57 35.50775002066659 38.5 35.0 40.0 28.0 41.0 58-59 35.21164835812216 38.0 34.0 40.0 27.5 41.0 60-61 34.84818271740545 38.0 34.0 40.0 26.5 41.0 62-63 34.50359912316625 37.0 34.0 40.0 26.0 41.0 64-65 34.15021555021127 37.0 33.5 39.5 26.0 41.0 66-67 33.74547762639167 36.0 33.0 39.0 25.5 40.5 68-69 33.300450579282284 36.0 33.0 39.0 25.0 40.0 70-71 32.814112766444666 35.0 32.0 38.0 23.5 40.0 72-73 32.4583395649848 35.0 32.0 37.5 22.5 39.5 74-75 32.04639037725325 35.0 31.5 37.0 22.0 39.0 76-77 29.996302247290302 33.0 29.0 35.0 19.5 37.5 78-79 31.073810150481318 34.0 31.0 36.0 20.0 38.0 80-81 31.12301831107834 34.0 31.0 36.0 20.0 37.0 82-83 30.824628880212614 34.0 31.0 35.0 19.5 37.0 84-85 30.45174696866184 34.0 31.0 35.0 18.0 36.5 86-87 29.95598917925587 34.0 30.0 35.0 12.0 36.0 88-89 29.458178127996064 34.0 29.5 35.0 7.0 36.0 90-91 29.05572084608565 34.0 29.0 35.0 2.0 35.0 92-93 28.797931155058375 34.0 29.0 35.0 2.0 35.0 94-95 28.42715600096159 34.0 29.0 35.0 2.0 35.0 96-97 27.926653541399226 34.0 29.0 35.0 2.0 35.0 98-99 27.23182123537779 33.0 27.0 35.0 2.0 35.0 100-101 24.050548116109205 30.0 13.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -3.4823145907125763E-4 1101 2 0.024799002754541277 1101 3 0.12241695911632178 1101 4 0.08502802935797149 1101 5 0.08505338943741236 1101 6 0.16036727989615684 1101 7 0.12133985508975087 1101 8 0.13312539173605842 1101 9 0.20766777071689546 1101 10-11 0.14864627140273967 1101 12-13 0.17731178453566798 1101 14-15 0.2438845907412599 1101 16-17 0.23667882015880792 1101 18-19 0.255592413799441 1101 20-21 0.23992799013905852 1101 22-23 0.25800291793131436 1101 24-25 0.2511106209754672 1101 26-27 0.32268719565165327 1101 28-29 0.3201045548470205 1101 30-31 0.36476411959381494 1101 32-33 0.2880752566092539 1101 34-35 0.21227784851851084 1101 36-37 0.199387467040836 1101 38-39 0.2027429409874273 1101 40-41 0.3173907864361283 1101 42-43 0.2515889646656788 1101 44-45 0.30938194804667063 1101 46-47 0.2270846686135215 1101 48-49 0.126958380058106 1101 50-51 0.21781367969042975 1101 52-53 0.2905474780948012 1101 54-55 0.1829413859383564 1101 56-57 0.12187546688812034 1101 58-59 0.10558571955031226 1101 60-61 0.10035872332878881 1101 62-63 0.06245818653268742 1101 64-65 0.021918496955876776 1101 66-67 -0.00422443911339343 1101 68-69 0.043623778189704865 1101 70-71 0.07217708150876234 1101 72-73 0.1514550366076719 1101 74-75 0.22468132389153794 1101 76-77 0.012271494303018216 1101 78-79 0.012597960423246235 1101 80-81 0.1361534194964662 1101 82-83 0.264715821256587 1101 84-85 0.3169876896833763 1101 86-87 0.4113648467530524 1101 88-89 0.5472385563904787 1101 90-91 0.47881656378485715 1101 92-93 0.5438461858269612 1101 94-95 0.599735418705837 1101 96-97 0.44973394490487095 1101 98-99 0.5724517518362262 1101 100-101 1.112203418318817 1102 1 0.07745487591380851 1102 2 0.08381033815336991 1102 3 0.153388147459097 1102 4 0.08139814376747978 1102 5 0.036118756539480046 1102 6 0.07011659773250756 1102 7 0.10913492246455547 1102 8 0.10334031310398473 1102 9 0.1157739842740142 1102 10-11 0.18764525439766544 1102 12-13 0.1536211290483962 1102 14-15 0.1999231316995278 1102 16-17 0.23602082563831317 1102 18-19 0.1845463201877422 1102 20-21 0.23907905953171138 1102 22-23 0.10875793762082964 1102 24-25 0.012311267028053408 1102 26-27 0.10342101149052496 1102 28-29 0.09932558157363758 1102 30-31 0.12035649850610497 1102 32-33 0.18469733216494433 1102 34-35 0.13957997351550944 1102 36-37 0.15179639975922044 1102 38-39 0.22900574606715907 1102 40-41 0.16197202108378406 1102 42-43 0.15763462164827047 1102 44-45 -0.012996854885891196 1102 46-47 0.020221722766471828 1102 48-49 0.1340862512114711 1102 50-51 0.09828352906368565 1102 52-53 0.1304884388658465 1102 54-55 0.019976306599438942 1102 56-57 -0.1279032469435677 1102 58-59 -0.06970723797874712 1102 60-61 -0.13544758165894422 1102 62-63 -0.2958077255078635 1102 64-65 -0.18478635904130414 1102 66-67 -0.15774747339884243 1102 68-69 -0.06322451562063947 1102 70-71 -0.023127325053643233 1102 72-73 0.017061785451481626 1102 74-75 0.02278644072509195 1102 76-77 -0.0986567062794812 1102 78-79 -0.09467174783402044 1102 80-81 -0.08994826864955385 1102 82-83 -0.01349077578926483 1102 84-85 0.003616561121539519 1102 86-87 -0.0405973137480089 1102 88-89 0.17110599515899239 1102 90-91 0.23017070834601938 1102 92-93 0.26550376622337524 1102 94-95 0.26170433388298164 1102 96-97 0.27641829914468374 1102 98-99 0.37221887241226526 1102 100-101 0.8550851751939028 1103 1 0.042362565328758706 1103 2 0.04939869924379536 1103 3 -0.03434324818784518 1103 4 0.04150629253881988 1103 5 0.06573289262725979 1103 6 0.022108141398994974 1103 7 0.10547236882771216 1103 8 0.056362445965106645 1103 9 0.057575204004422176 1103 10-11 0.03186116365689884 1103 12-13 0.048959384348442825 1103 14-15 0.10378210784611497 1103 16-17 0.063494108422681 1103 18-19 0.06066801373366815 1103 20-21 0.06521482580483706 1103 22-23 -0.00496570677894681 1103 24-25 0.08141006227563707 1103 26-27 0.10703743981433433 1103 28-29 0.19960069927547863 1103 30-31 0.05573121200598763 1103 32-33 0.17330741698457786 1103 34-35 0.029150115381611386 1103 36-37 -0.06747891004651763 1103 38-39 0.0823590328111834 1103 40-41 0.14361522419168438 1103 42-43 0.22661543876936463 1103 44-45 0.21929051545475176 1103 46-47 0.18684500734178044 1103 48-49 0.25149995984261153 1103 50-51 0.24797102516928504 1103 52-53 0.15016901656638737 1103 54-55 0.10245730136372799 1103 56-57 -0.029059620689807275 1103 58-59 -0.09096191546562693 1103 60-61 -0.005806158353266255 1103 62-63 0.09398056126476462 1103 64-65 0.11911667324539366 1103 66-67 0.002214524359395398 1103 68-69 0.02466611228791038 1103 70-71 -0.12257146212662207 1103 72-73 -0.054669620854838286 1103 74-75 0.09334022115496765 1103 76-77 0.07697382910098227 1103 78-79 0.015709320142935468 1103 80-81 0.11391863932656676 1103 82-83 0.07059114355045537 1103 84-85 0.00442187303329078 1103 86-87 0.05496596802608167 1103 88-89 0.188371989140677 1103 90-91 0.29335045418418204 1103 92-93 0.2223548683048442 1103 94-95 0.04052334716809369 1103 96-97 0.17978055622358724 1103 98-99 0.35709801899756854 1103 100-101 1.051456856963572 1104 1 -0.05334981607794376 1104 2 -0.03420720401152977 1104 3 -0.017724371072830536 1104 4 -0.04245332681298919 1104 5 -0.0313952513184006 1104 6 0.018485616845445918 1104 7 -0.019523823784410865 1104 8 -0.037566281608206964 1104 9 0.008877775830505641 1104 10-11 0.007976247988786156 1104 12-13 -0.022633233895753335 1104 14-15 -0.04981762314533 1104 16-17 0.045319825496356714 1104 18-19 0.033141985649130845 1104 20-21 -0.004144613010403475 1104 22-23 -0.04159633173569688 1104 24-25 -0.06434512198084974 1104 26-27 -0.05749211074831351 1104 28-29 -0.048101395701628746 1104 30-31 -0.05742697211288572 1104 32-33 -0.10408099843447616 1104 34-35 0.03352537028135316 1104 36-37 -0.06458687678892261 1104 38-39 0.09797174422273969 1104 40-41 0.0423054359011914 1104 42-43 0.1251343180243225 1104 44-45 0.10363974187944791 1104 46-47 0.17826272551135247 1104 48-49 0.12602220100154682 1104 50-51 0.14154252603924533 1104 52-53 0.06107961566258524 1104 54-55 0.05589886370015762 1104 56-57 0.0400017679922442 1104 58-59 0.05331276113290073 1104 60-61 0.10287908696044923 1104 62-63 -0.045268812410164116 1104 64-65 0.16722961856724083 1104 66-67 0.11290477918024777 1104 68-69 0.1641982870194454 1104 70-71 0.011400891473250852 1104 72-73 -0.030463244499358666 1104 74-75 0.017714212392426987 1104 76-77 -0.04050413920499096 1104 78-79 -0.05234361840293644 1104 80-81 -0.05536239463345538 1104 82-83 -0.09535932416859438 1104 84-85 -0.0010499681507596392 1104 86-87 0.2252784526500946 1104 88-89 0.42020432521173845 1104 90-91 0.367024971645602 1104 92-93 0.4053246917685698 1104 94-95 0.38542985099883253 1104 96-97 0.24219502667177295 1104 98-99 0.4302063876439419 1104 100-101 0.9575721193640554 1105 1 -0.028436045372124852 1105 2 -0.015244180320244993 1105 3 -0.02158850840807247 1105 4 -0.056639656145698325 1105 5 -0.008917031655336416 1105 6 -0.10702356518581269 1105 7 -0.03540115310763525 1105 8 -0.004227577592189391 1105 9 0.04261843948775379 1105 10-11 0.0017120963929357913 1105 12-13 -0.05820593534301821 1105 14-15 -1.2691173337486816E-4 1105 16-17 0.010915859576165587 1105 18-19 -0.06473208852015233 1105 20-21 -0.006942111343349211 1105 22-23 -0.02052038048987015 1105 24-25 -0.04910887008695397 1105 26-27 -0.06480423401634994 1105 28-29 -0.09596618975966464 1105 30-31 -0.16928100215994135 1105 32-33 -0.049434722918761054 1105 34-35 0.017504669742358203 1105 36-37 0.012042781130723768 1105 38-39 0.002467847188064809 1105 40-41 0.02633247476381939 1105 42-43 0.06386871435500296 1105 44-45 0.11261914844808274 1105 46-47 0.20437526013389373 1105 48-49 0.1699043507802429 1105 50-51 0.15837238648412466 1105 52-53 0.1470896452212287 1105 54-55 0.15683292640586188 1105 56-57 0.03903148760588948 1105 58-59 0.05486555402560356 1105 60-61 0.035817506804612265 1105 62-63 0.043265131987510586 1105 64-65 0.0373315100574203 1105 66-67 0.1307665068998034 1105 68-69 0.09134711858229139 1105 70-71 0.03839381257685659 1105 72-73 -0.11022235790521506 1105 74-75 -0.1363045730621124 1105 76-77 -0.07505778804068441 1105 78-79 -0.11242411437859445 1105 80-81 -0.04014676095869518 1105 82-83 -0.06663370760351128 1105 84-85 0.0731736698303429 1105 86-87 0.14483790518932693 1105 88-89 0.23391779730147633 1105 90-91 0.3284387587235891 1105 92-93 0.24577050739472028 1105 94-95 0.3060835227555607 1105 96-97 0.2392805132510354 1105 98-99 0.2687836341270611 1105 100-101 0.7960216399962015 1106 1 -0.03504977431655831 1106 2 -0.07986938567294999 1106 3 -0.04726763887369856 1106 4 -0.03949492201208926 1106 5 -0.01620998959225517 1106 6 -0.09592724320853563 1106 7 -0.08995001682013282 1106 8 -0.054107101983348116 1106 9 -0.04972767596207461 1106 10-11 -0.05928963222287109 1106 12-13 -0.03177436285135826 1106 14-15 -0.03396891649116185 1106 16-17 -0.08739192650110539 1106 18-19 -0.11187067154631336 1106 20-21 -0.08392955720284334 1106 22-23 -0.09089860690337304 1106 24-25 -0.06474978122695063 1106 26-27 -0.03703140784470804 1106 28-29 -0.09726484952156511 1106 30-31 -0.052731499083925826 1106 32-33 -0.013057887401316748 1106 34-35 0.037767968366949844 1106 36-37 0.011011887049519942 1106 38-39 0.06464841174997815 1106 40-41 0.019452268566816144 1106 42-43 0.051175347819629735 1106 44-45 0.1534934697117265 1106 46-47 0.13293184471405084 1106 48-49 0.17335138865366417 1106 50-51 0.1530520499042538 1106 52-53 0.09871921122103089 1106 54-55 0.12197897503932609 1106 56-57 0.035478154412004415 1106 58-59 0.09139112720180265 1106 60-61 0.13171146068130923 1106 62-63 0.1470760219639331 1106 64-65 0.16099446271632445 1106 66-67 0.1671068116903598 1106 68-69 0.1441925287314021 1106 70-71 0.19952505095637463 1106 72-73 0.1933225171369557 1106 74-75 0.28183688907038373 1106 76-77 0.17369482405786485 1106 78-79 0.16953053264818152 1106 80-81 0.1561013818100534 1106 82-83 0.3134177264473088 1106 84-85 0.1385560919721449 1106 86-87 0.31104183668375285 1106 88-89 0.5414668038021269 1106 90-91 0.49254001341005704 1106 92-93 0.5586542884492438 1106 94-95 0.6090170007566371 1106 96-97 0.45900453649366924 1106 98-99 0.5485889556686985 1106 100-101 1.0897876866106202 1107 1 -0.07232210147026308 1107 2 -0.02432243105863563 1107 3 -0.10295220854784759 1107 4 -0.04036287561682883 1107 5 -0.024846817517669706 1107 6 -0.03445661275360834 1107 7 -0.06809324562533448 1107 8 0.058289849843077945 1107 9 0.007247628066366474 1107 10-11 -0.07913000171930662 1107 12-13 -0.02970020138381102 1107 14-15 -0.04696486455809179 1107 16-17 -0.01073989329791658 1107 18-19 -0.023021020744529608 1107 20-21 0.021546306183360286 1107 22-23 -0.07220149527739039 1107 24-25 -0.05314837197755651 1107 26-27 -0.05411999516680055 1107 28-29 -0.009973452005048955 1107 30-31 -0.19681246478575076 1107 32-33 -0.06359368155290923 1107 34-35 -0.029470260503501322 1107 36-37 -0.06805382236204593 1107 38-39 -0.08152406559395331 1107 40-41 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0.6214664226919808 2201 40-41 0.6443724622697786 2201 42-43 0.7057973463510052 2201 44-45 0.7357165113168875 2201 46-47 0.6882477899338753 2201 48-49 0.6411176331118611 2201 50-51 0.7276758716844967 2201 52-53 0.7175809400368891 2201 54-55 0.8060190313783409 2201 56-57 0.7796237868851321 2201 58-59 0.6829719466237236 2201 60-61 0.7331689946463698 2201 62-63 0.7037503189166685 2201 64-65 0.6567035624198425 2201 66-67 0.626420480668564 2201 68-69 0.5936107651046569 2201 70-71 0.5813326299074362 2201 72-73 0.5650091852404984 2201 74-75 0.5641723017010492 2201 76-77 0.6239642231751894 2201 78-79 0.5753481375883709 2201 80-81 0.46618659519909045 2201 82-83 0.3447484725633494 2201 84-85 0.33302234369827843 2201 86-87 0.3000252783260038 2201 88-89 0.2614236097207332 2201 90-91 0.23171102726173487 2201 92-93 -0.2044013638728579 2201 94-95 -0.773284139807707 2201 96-97 -0.3308052566709634 2201 98-99 -0.06780175403248734 2201 100-101 0.2248162602410524 2202 1 0.3535856615684807 2202 2 0.32972291554946054 2202 3 0.35789061363968955 2202 4 0.1916369004076799 2202 5 0.21808531068301562 2202 6 0.26962564164401925 2202 7 0.29337159022229997 2202 8 0.29382566906355123 2202 9 0.3906575465681854 2202 10-11 0.38321457012624194 2202 12-13 0.3383056821874746 2202 14-15 0.44632806287384597 2202 16-17 0.4934843326620495 2202 18-19 0.45839473316180346 2202 20-21 0.41626668602653893 2202 22-23 0.38142639593313277 2202 24-25 0.4563075180962386 2202 26-27 0.4405872291265922 2202 28-29 0.4575708734674677 2202 30-31 0.47291354454897316 2202 32-33 0.37487281484420976 2202 34-35 0.5231266510430359 2202 36-37 0.4691632881614325 2202 38-39 0.5607652469216973 2202 40-41 0.7046039786115088 2202 42-43 0.5499003063819359 2202 44-45 0.5974681088585001 2202 46-47 0.5690767181564311 2202 48-49 0.534765052115489 2202 50-51 0.5080135812982363 2202 52-53 0.49057485513315413 2202 54-55 0.5114242803709814 2202 56-57 0.42660524726203164 2202 58-59 0.39937969113917404 2202 60-61 0.36799686462479286 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-0.8314320392736221 2316 60-61 -0.7795865077502171 2316 62-63 -0.7984962518778858 2316 64-65 -0.826406610124927 2316 66-67 -0.9750127259487513 2316 68-69 -0.9039943928071992 2316 70-71 -0.9200730797170209 2316 72-73 -1.1103375870987335 2316 74-75 -1.0503151658226528 2316 76-77 -1.0141426821871882 2316 78-79 -0.87221055438118 2316 80-81 -0.9115446109093455 2316 82-83 -1.1865795246544941 2316 84-85 -1.2821231550850491 2316 86-87 -1.4942903717709441 2316 88-89 -1.8842115831974944 2316 90-91 -2.3175289731918767 2316 92-93 -2.3869123770083256 2316 94-95 -2.301683208703089 2316 96-97 -2.38722799109863 2316 98-99 -2.9746480653294682 2316 100-101 -3.736409607058029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 25.0 4 43.0 5 140.0 6 299.0 7 795.0 8 1885.0 9 3196.0 10 5544.0 11 8385.0 12 11893.0 13 16485.0 14 19310.0 15 25529.0 16 32905.0 17 44867.0 18 42402.0 19 28708.0 20 26472.0 21 28258.0 22 29860.0 23 33468.0 24 39381.0 25 47464.0 26 58066.0 27 71161.0 28 87350.0 29 107464.0 30 130816.0 31 159441.0 32 195954.0 33 248149.0 34 328507.0 35 459679.0 36 706666.0 37 1140635.0 38 1014346.0 39 106560.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92897563101219 19.71424804814006 14.914302841207515 22.442473479640235 2 15.446735770692962 21.74045987212366 38.94052106424888 23.872283292934497 3 20.13756825915055 25.13652780298416 30.108349969546467 24.617553968318823 4 13.034853856291626 17.19774273272249 37.57513851369649 32.1922648972894 5 13.418539883677951 38.225637410052805 33.93447311584791 14.421349590421343 6 30.938833529863942 36.68293072272271 17.444240576270186 14.933995171143161 7 27.944505203706115 33.2834421140549 20.914119132706148 17.857933549532838 8 26.018511568067215 34.12698126133694 19.817506838980144 20.037000331615708 9 26.06742726071171 17.645509457699042 20.96997119979324 35.317092081796005 10-11 25.039114500538282 26.49657029540403 27.2322725748107 21.232042629246987 12-13 26.640466808498104 24.07917728897981 27.447319186296763 21.833036716225322 14-15 22.941008566346955 25.476240202823853 26.874207897488894 24.7085433333403 16-17 21.798686277982725 28.74782687025988 27.14912744137396 22.304359410383437 18-19 21.580205419616135 27.929553693986076 29.11158020922037 21.37866067717741 20-21 23.017351388177566 26.26906671556969 28.692683455226653 22.02089844102609 22-23 22.351278905915205 26.40659696718905 28.827857239912085 22.414266886983654 24-25 22.764202226671216 26.541172133258208 28.86890537359978 21.825720266470803 26-27 22.44124834405686 26.220057858952693 29.58195262848133 21.756741168509116 28-29 21.65763195977283 27.510307167365212 29.036129008963123 21.795931863898833 30-31 22.178658201122552 26.457717096642703 28.872231032579105 22.491393669655643 32-33 22.096181858435248 26.73275859611582 28.99292394788027 22.17813559756866 34-35 22.88025386542012 26.518033052336555 28.76774942173948 21.833963660503844 36-37 22.675293205085183 26.10245387631145 29.263439859279426 21.95881305932394 38-39 22.195733246564963 26.876730251611797 29.137104539032272 21.790431962790965 40-41 22.32357200870707 26.884663997884335 28.807359901993664 21.984404091414934 42-43 22.037357438077784 27.033543243878093 28.128266390772655 22.80083292727147 44-45 22.449589857414793 26.492806030545545 28.20166774626015 22.85593636577951 46-47 22.894812408198575 27.077118613951214 27.99099474215024 22.037074235699976 48-49 22.781772529568688 26.983678133069184 27.9069575194373 22.327591817924837 50-51 21.873065168452207 27.26742778434256 28.403279611925765 22.45622743527947 52-53 22.551559591533064 27.701656083152876 27.305627110011848 22.441157215302212 54-55 22.00755779757759 26.926616008962174 27.782840169779643 23.282986023680593 56-57 22.604599101311926 27.02681336306789 27.56944688590044 22.799140649719742 58-59 23.292309733028283 26.70629348436069 27.368102388156196 22.633294394454833 60-61 22.533314088708913 26.98482756471282 28.3635231003432 22.118335246235066 62-63 22.21872764088204 26.99757606969821 28.195288396395746 22.588407893024005 64-65 21.910296525256477 27.625289451104734 27.682937374040666 22.78147664959812 66-67 22.25115893333379 27.10884913195093 27.234113797860665 23.405878136854614 68-69 21.896976543562154 27.057651323190374 27.682585581520286 23.362786551727186 70-71 22.49894123289047 26.8790206188065 27.49265419445188 23.12938395385115 72-73 22.346784515351718 26.511260206209858 28.05237057722988 23.089584701208544 74-75 21.75863697391638 27.71670706550503 27.35305100705705 23.17160495352154 76-77 21.98897688707344 26.768537931411 27.539881908670445 23.70260327284511 78-79 21.995885968655156 26.95219018797536 27.658082761779028 23.393841081590455 80-81 22.262318082769625 26.854913659659246 27.49456183131049 23.388206426260638 82-83 22.008726529161756 26.662368648347666 27.96857347283364 23.360331349656935 84-85 21.695744011675913 26.929257532380042 27.72139149372448 23.653606962219563 86-87 21.67744242789563 26.880650993714596 27.65660287112758 23.78530370726219 88-89 21.54164495833669 26.875902395217288 27.227198388475273 24.355254257970753 90-91 21.684633272741618 26.379331834358588 27.533150427413627 24.402884465486164 92-93 22.02286533076529 26.725869731087215 27.51141888765259 23.739846050494904 94-95 21.651222303431663 26.379255561548078 27.662038488713854 24.30748364630641 96-97 21.016519409598388 26.31843899969461 28.193427241110175 24.471614349596827 98-99 21.260071537897566 26.65888904556465 27.274555092580805 24.806484323956987 100-101 21.051941437606423 25.84779212782778 28.016478274750668 25.08378815981513 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1062.0 1 749.0 2 559.5 3 714.5 4 660.0 5 693.0 6 1203.0 7 2543.5 8 4107.5 9 4799.0 10 4485.5 11 4170.5 12 4396.0 13 4853.0 14 5401.0 15 5878.0 16 6296.0 17 6678.5 18 7173.5 19 7878.5 20 8842.5 21 10158.5 22 11837.5 23 13821.0 24 16405.0 25 20346.5 26 26571.5 27 34105.5 28 40832.5 29 48290.5 30 57971.0 31 68790.5 32 78872.0 33 88529.0 34 99878.0 35 109165.5 36 119712.5 37 132005.0 38 138791.5 39 142099.5 40 146025.0 41 153244.5 42 160270.5 43 167430.0 44 175903.5 45 182766.5 46 187233.5 47 215659.5 48 278513.0 49 279239.5 50 224582.5 51 195467.0 52 181221.0 53 175567.0 54 169705.0 55 157368.0 56 142933.0 57 128134.5 58 115883.5 59 103101.5 60 87045.5 61 70153.5 62 55758.0 63 43986.0 64 33077.5 65 24679.5 66 17902.0 67 13762.5 68 10069.5 69 6527.0 70 4710.0 71 3492.0 72 2727.5 73 2246.5 74 1741.0 75 1304.0 76 872.0 77 444.5 78 230.5 79 136.0 80 72.5 81 42.0 82 27.0 83 19.5 84 12.0 85 9.5 86 10.5 87 4.5 88 3.5 89 2.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39102148090644157 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.2804518715383455E-4 16-17 0.0022614481059421926 18-19 1.5203012476922302E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.5203012476922302E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008162117323547661 36-37 0.01726492104410489 38-39 0.011345248060903269 40-41 0.007877060839605368 42-43 0.0036297192288652 44-45 0.001966889739201823 46-47 0.0014822937164999245 48-49 4.750941399038219E-5 50-51 5.511092022884334E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.1808284311536335E-4 60-61 7.506487410480387E-4 62-63 0.0 64-65 0.0 66-67 0.0010737127561826377 68-69 7.886562722403444E-4 70-71 0.0018718709112210583 72-73 0.0 74-75 0.0 76-77 0.09979827502819684 78-79 0.034672370330180925 80-81 6.651317958653507E-5 82-83 0.0 84-85 0.0 86-87 0.15424406346117484 88-89 0.3305229931310889 90-91 0.284543382271197 92-93 0.0 94-95 1.9003765596152878E-5 96-97 3.8007531192305756E-5 98-99 1.710338903653759E-4 100-101 6.651317958653507E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5262115.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.30132032992341 #Duplication Level Percentage of deduplicated Percentage of total 1 69.14743109935868 29.2502763292528 2 15.299362376505421 12.943664574642682 3 6.048521267379891 7.675813069613733 4 2.9969497550677313 5.070997264072225 5 1.7140395479035466 3.6253067987012515 6 1.0342634534125188 2.625042578900147 7 0.6976682026874149 2.065860028811759 8 0.5062244136040611 1.7131168862954422 9 0.386974165062269 1.473256630413333 >10 1.9125173078870503 14.592311278132852 >50 0.14856533332450347 4.342900722647037 >100 0.09920228637106718 7.669336609161956 >500 0.005760552333263115 1.6833072892580216 >1k 0.0018451750573544878 1.5856631444849163 >5k 3.6003415753201463E-4 1.2443796695861993 >10k+ 3.150298878405128E-4 2.438767126025709 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 38910 0.7394365193463085 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18718 0.35571248442878955 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18037 0.34277092005780946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14480 0.2751745258322937 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13886 0.2638862890681789 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12936 0.24583271175183363 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11137 0.21164493744435459 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9828 0.18676900827899048 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9378 0.17821731376072167 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9369 0.17804627987035632 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8844 0.16806930293237604 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7895 0.15003472938162696 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7244 0.13766327797853145 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6686 0.12705917677587814 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6121 0.11632204921405176 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9003765596152878E-5 0.0 5 0.0 0.0 0.0 1.9003765596152878E-5 0.0 6 0.0 0.0 0.0 1.9003765596152878E-5 0.0 7 1.9003765596152878E-5 0.0 0.0 1.9003765596152878E-5 0.0 8 1.9003765596152878E-5 0.0 0.0 1.9003765596152878E-5 0.0 9 1.9003765596152878E-5 0.0 0.0 3.8007531192305756E-5 0.0 10-11 3.8007531192305756E-5 0.0 0.0 6.651317958653507E-5 0.0 12-13 5.701129678845864E-5 0.0 0.0 9.501882798076439E-5 0.0 14-15 9.501882798076439E-5 0.0 0.0 1.235244763749937E-4 0.0 16-17 9.501882798076439E-5 0.0 0.0 1.6153200756729947E-4 0.0 18-19 9.501882798076439E-5 0.0 0.0 1.9003765596152877E-4 0.0 20-21 9.501882798076439E-5 0.0 0.0 2.3754706995191097E-4 0.0 22-23 1.1402259357691726E-4 0.0 0.0 5.986186162788156E-4 1.9003765596152878E-5 24-25 1.710338903653759E-4 0.0 0.0 0.0011117202873749434 3.8007531192305756E-5 26-27 1.9003765596152877E-4 0.0 0.0 0.0019193803252114405 3.8007531192305756E-5 28-29 2.2804518715383452E-4 1.9003765596152878E-5 0.0 0.00525454118733627 3.8007531192305756E-5 30-31 2.470489527499874E-4 1.9003765596152878E-5 0.0 0.015459563312470366 3.8007531192305756E-5 32-33 2.470489527499874E-4 1.9003765596152878E-5 0.0 0.037903010481526916 3.8007531192305756E-5 34-35 2.755546011442167E-4 1.9003765596152878E-5 0.0 0.07003837810462143 3.8007531192305756E-5 36-37 2.8505648394229314E-4 2.850564839422932E-5 0.0 0.12177612994014764 3.8007531192305756E-5 38-39 3.420677807307518E-4 3.8007531192305756E-5 0.0 0.20045171950822055 3.8007531192305756E-5 40-41 3.5156966352882827E-4 3.8007531192305756E-5 0.0 0.28997845923169674 3.8007531192305756E-5 42-43 3.8957719472113397E-4 3.8007531192305756E-5 0.0 0.3837715443315093 3.8007531192305756E-5 44-45 4.5609037430766904E-4 3.8007531192305756E-5 0.0 0.49905788832057074 3.8007531192305756E-5 46-47 5.511092022884334E-4 3.8007531192305756E-5 0.0 0.6363790985183715 4.750941399038219E-5 48-49 5.891167334807392E-4 3.8007531192305756E-5 0.0 0.7778716352645276 5.701129678845863E-5 50-51 5.891167334807392E-4 3.8007531192305756E-5 0.0 0.9354508595878273 5.701129678845863E-5 52-53 5.986186162788157E-4 4.750941399038219E-5 0.0 1.095405554610646 5.701129678845863E-5 54-55 6.461280302691979E-4 5.701129678845863E-5 0.0 1.2701261755016757 5.701129678845863E-5 56-57 6.841355614615036E-4 5.701129678845863E-5 0.0 1.4847927116758184 5.701129678845863E-5 58-59 6.841355614615036E-4 7.601506238461151E-5 0.0 1.7142156718353743 5.701129678845863E-5 60-61 6.841355614615036E-4 7.601506238461151E-5 0.0 1.9619297563812268 5.701129678845863E-5 62-63 6.841355614615036E-4 7.601506238461151E-5 0.0 2.216143128760964 5.701129678845863E-5 64-65 6.841355614615036E-4 7.601506238461151E-5 0.0 2.48019094983671 5.701129678845863E-5 66-67 7.031393270576564E-4 7.601506238461151E-5 0.0 2.781400634535733 5.701129678845863E-5 68-69 7.221430926538094E-4 8.551694518268795E-5 0.0 3.1266420441210423 5.701129678845863E-5 70-71 7.506487410480387E-4 9.501882798076439E-5 0.0 3.498241676588216 5.701129678845863E-5 72-73 7.601506238461151E-4 9.501882798076439E-5 0.0 3.8701073617737354 5.701129678845863E-5 74-75 7.601506238461151E-4 9.501882798076439E-5 0.0 4.2641314376443695 5.701129678845863E-5 76-77 7.601506238461151E-4 9.501882798076439E-5 0.0 4.680532447504473 5.701129678845863E-5 78-79 7.601506238461151E-4 9.501882798076439E-5 0.0 5.163142576701574 5.701129678845863E-5 80-81 7.981581550384209E-4 9.501882798076439E-5 0.0 5.680862162837567 6.651317958653507E-5 82-83 8.171619206345737E-4 9.501882798076439E-5 0.0 6.193707283098146 7.601506238461151E-5 84-85 8.456675690288031E-4 9.501882798076439E-5 0.0 6.720045076931994 7.601506238461151E-5 86-87 8.64671334624956E-4 9.501882798076439E-5 0.0 7.2658332248535045 7.601506238461151E-5 88-89 8.931769830191852E-4 9.501882798076439E-5 0.0 7.852412955627158 7.601506238461151E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 25655 0.0 23.209919 1 TACATGG 26020 0.0 22.486029 2 ACATGGG 25945 0.0 21.691422 3 GAGTACT 17730 0.0 21.131147 12-13 CATGGGG 10860 0.0 20.40535 4 AGTACTT 18770 0.0 20.301632 12-13 GTACTTT 19545 0.0 19.302485 14-15 AGAGTAC 35655 0.0 18.06737 10-11 CATGGGA 14095 0.0 17.338005 4 CGCGGAA 555 0.0 17.21145 82-83 ATGGGGA 5950 0.0 16.508604 5 ACTTTTT 23220 0.0 16.379955 16-17 TACTTTT 23055 0.0 16.312325 14-15 TCGCGCT 615 0.0 15.817573 60-61 GGACCGT 480 2.1827873E-11 15.817421 6 ATTCGCG 640 0.0 15.236545 80-81 AGTACAT 20110 0.0 15.219648 2 TATACCG 380 6.97255E-8 14.984926 5 GAGTACA 23985 0.0 14.935293 1 GTACACG 705 0.0 14.865146 1 >>END_MODULE