FastQCFastQC Report
Thu 26 May 2016
SRR938736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5274182
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT358850.6803898689882146No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT204420.38758616976054294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184640.3500827237285327No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT147950.2805174337935248No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC139610.2647045551329097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134060.2541815963119968No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT109490.20759617320752297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89440.16958079944908994No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT87150.16523889391757812No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC82270.1559862742696403No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC81400.15433672937338908No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG69160.1311293391088893No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC65810.12477764324401396No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA65610.1243984375207378No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA55920.10602592022800882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG264100.024.6199421
TACATGG269900.023.764742
ACATGGG268600.023.1202723
GAGTACT171900.022.25666212-13
AGTACTT181400.020.41071512-13
CATGGGG114700.020.0189464
GTACTTT189500.020.00178114-15
CATGGGA142850.019.627574
AGAGTAC347300.018.48374710-11
GTATAGG13700.018.0406481
ACTTTTT227650.016.94152516-17
TACTTTT219650.016.79162614-15
AGTACAT204150.016.1043472
GTATAGA14800.015.7363391
TACCTGG39900.015.6949632
GAGTACA242650.015.59201051
GTGTAAG22150.015.0208211
ATGGGGA61300.015.0141425
ATGGGAT64250.014.9893295
ATGGGAG60500.014.5069345
Produced by FastQC (version 0.11.5)