FastQCFastQC Report
Thu 26 May 2016
SRR938736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5274182
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT391520.7423331238853722No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT191120.3623689891626797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178950.3392943209013265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145330.2755498388186073No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT137560.26081769646932923No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC132860.25190636197234No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT112600.213492822204467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95950.1819239457417283No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC94700.17955390997125242No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC91180.17287988924159234No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT89180.16908783200883093No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG77370.14669573404937486No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA72970.1383532081372998No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC66990.1270149570113432No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA60610.1149182944388343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG254850.023.5019191
GAGTACT177900.022.53618212-13
TACATGG262200.022.4280032
ACATGGG259550.021.3586483
GTACTTT196300.020.29083814-15
AGTACTT190100.020.25377712-13
CATGGGG109900.019.3046444
GGACCGT5000.018.97846
AGAGTAC352850.018.46877710-11
ACTTTTT231000.017.54022616-17
CATGGGA141300.017.5339724
TACTTTT226450.016.82452414-15
AGTACAT199600.015.931852
GTACCGT4208.785719E-1015.8153336
GAGTACA237500.015.3035431
ACCGTAT4101.0877557E-815.043148
TACCTGG44450.014.9488662
ATGGGGA58000.014.7297875
ATGGGAT59250.014.6593495
TACACTG38950.014.5007835
Produced by FastQC (version 0.11.5)