##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5274182 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09125642611499 33.0 31.0 34.0 30.0 34.0 2 32.28670246874302 34.0 31.0 34.0 30.0 34.0 3 32.374206085417605 34.0 31.0 34.0 30.0 34.0 4 35.97434445758603 37.0 35.0 37.0 35.0 37.0 5 35.88404761155379 37.0 35.0 37.0 35.0 37.0 6 35.9140340625333 37.0 35.0 37.0 35.0 37.0 7 35.836268259229584 37.0 35.0 37.0 35.0 37.0 8 35.82525441859989 37.0 35.0 37.0 33.0 37.0 9 37.46715566508702 39.0 37.0 39.0 34.0 39.0 10-11 37.394994332770466 39.0 37.0 39.0 34.0 39.0 12-13 37.3232396037907 39.0 37.0 39.0 34.0 39.0 14-15 38.53070703665516 40.0 38.0 41.0 34.0 41.0 16-17 38.474010946152404 40.0 38.0 41.0 34.0 41.0 18-19 38.634082782884626 40.0 38.0 41.0 34.0 41.0 20-21 38.639064503272735 40.0 38.0 41.0 34.0 41.0 22-23 38.56182645953439 40.0 38.0 41.0 34.0 41.0 24-25 38.479466863297475 40.0 38.0 41.0 34.0 41.0 26-27 38.30497866778204 40.0 38.0 41.0 33.5 41.0 28-29 38.15071834836189 40.0 38.0 41.0 33.0 41.0 30-31 37.95307727340467 40.0 37.0 41.0 33.0 41.0 32-33 37.815755978841835 40.0 37.0 41.0 33.0 41.0 34-35 37.78022772062094 40.0 37.0 41.0 33.0 41.0 36-37 37.6753266193696 40.0 37.0 41.0 33.0 41.0 38-39 37.5593865551094 40.0 37.0 41.0 32.0 41.0 40-41 37.41752152276884 40.0 37.0 41.0 32.0 41.0 42-43 37.2227229739133 40.0 36.0 41.0 31.0 41.0 44-45 36.97556360398636 40.0 36.0 41.0 31.0 41.0 46-47 36.671699801030755 40.0 36.0 41.0 30.0 41.0 48-49 36.3656084488552 39.0 35.5 41.0 30.0 41.0 50-51 36.1397669818751 39.0 35.0 41.0 29.0 41.0 52-53 35.93509353677973 39.0 35.0 41.0 28.5 41.0 54-55 35.68049917503795 39.0 35.0 40.0 28.0 41.0 56-57 35.40173101724589 38.0 35.0 40.0 28.0 41.0 58-59 35.099747411067725 38.0 34.0 40.0 27.0 41.0 60-61 34.713018815050376 38.0 34.0 40.0 26.0 41.0 62-63 34.37866194226896 37.0 34.0 40.0 26.0 41.0 64-65 33.98533270182941 36.5 33.0 39.5 26.0 41.0 66-67 33.56901857008347 36.0 33.0 39.0 25.0 40.5 68-69 33.21854687608429 36.0 32.5 39.0 24.5 40.0 70-71 32.77066083422984 35.0 32.0 38.0 23.0 40.0 72-73 32.282906998658746 35.0 31.5 37.5 22.0 39.5 74-75 31.80418034493311 35.0 31.0 37.0 20.5 39.0 76-77 29.860246859133795 33.0 28.5 35.0 18.5 37.5 78-79 30.829453173970865 34.0 30.5 36.0 19.0 38.0 80-81 30.873702405415663 34.0 31.0 35.5 19.0 37.0 82-83 30.562446650494806 34.0 31.0 35.0 18.0 37.0 84-85 30.174102164089142 34.0 30.0 35.0 14.5 36.5 86-87 29.626566641045002 34.0 29.5 35.0 7.0 36.0 88-89 29.04021059569048 34.0 29.0 35.0 2.0 35.5 90-91 28.532222532328234 34.0 29.0 35.0 2.0 35.0 92-93 28.25254409119746 34.0 29.0 35.0 2.0 35.0 94-95 27.9195300806836 33.0 27.5 35.0 2.0 35.0 96-97 27.321139183289468 33.0 27.0 35.0 2.0 35.0 98-99 26.509693635904107 33.0 25.5 35.0 2.0 35.0 100-101 22.770287506195274 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10215154391906367 1101 2 0.17808960483558423 1101 3 0.06920511936166918 1101 4 0.08475743055786467 1101 5 -0.024339918200851685 1101 6 0.08723256295655091 1101 7 0.1870597697741232 1101 8 0.17885978038511752 1101 9 0.19980332732983896 1101 10-11 0.22019223624671724 1101 12-13 0.14951584234598414 1101 14-15 0.2667827299730803 1101 16-17 0.2985610625917232 1101 18-19 0.313055524530931 1101 20-21 0.24845060050694912 1101 22-23 0.1068074043770082 1101 24-25 0.1520935509923831 1101 26-27 0.1385655892615958 1101 28-29 0.2240280409304134 1101 30-31 0.19824543885319912 1101 32-33 0.2541384884738065 1101 34-35 0.35462723312063105 1101 36-37 0.34099541401813127 1101 38-39 0.34052837189857144 1101 40-41 0.3446554950270837 1101 42-43 0.37923607541017645 1101 44-45 0.38429394774932746 1101 46-47 0.3598822983781531 1101 48-49 0.2297902097202993 1101 50-51 0.26146407121473203 1101 52-53 0.2074010536908233 1101 54-55 0.22341774075516696 1101 56-57 0.3424744067200791 1101 58-59 0.36966577265737754 1101 60-61 0.37774694741516157 1101 62-63 0.26945540956138103 1101 64-65 0.2866976390880893 1101 66-67 0.28774078586437213 1101 68-69 0.22353858177814345 1101 70-71 0.326399196422706 1101 72-73 0.1739561238068319 1101 74-75 0.12465116683171118 1101 76-77 0.180051093174459 1101 78-79 0.032640243220317444 1101 80-81 0.19682113681346536 1101 82-83 0.2733443732138987 1101 84-85 0.31656529031501535 1101 86-87 0.29541874591143014 1101 88-89 0.36319253994838974 1101 90-91 0.16429529163916 1101 92-93 0.32507011415856724 1101 94-95 0.34823050895288077 1101 96-97 0.18117833134713024 1101 98-99 -0.01754173090681377 1101 100-101 0.5363500343456771 1102 1 0.25055120230479844 1102 2 0.14682576462924857 1102 3 0.15143776927711627 1102 4 0.1475706732232922 1102 5 -0.09308713690403891 1102 6 -7.91319743264296E-4 1102 7 0.11407350440953223 1102 8 0.12304190396086057 1102 9 0.18078692643679517 1102 10-11 0.14852758631612062 1102 12-13 0.19829072725947583 1102 14-15 0.2513770630394063 1102 16-17 0.306951858700657 1102 18-19 0.27958088711735485 1102 20-21 0.2247858218082186 1102 22-23 0.23816062481098754 1102 24-25 0.198071762668242 1102 26-27 0.16724690898337968 1102 28-29 0.30563080641525175 1102 30-31 0.21016379986220812 1102 32-33 0.25467774286848766 1102 34-35 0.20970596157327748 1102 36-37 0.2746724635616644 1102 38-39 0.2935512604327215 1102 40-41 0.2849322896854787 1102 42-43 0.2586045435050366 1102 44-45 0.2879760533353988 1102 46-47 0.2732623859026049 1102 48-49 0.3276662552511951 1102 50-51 0.19282253705614494 1102 52-53 0.13644947618661973 1102 54-55 0.20839762073569545 1102 56-57 0.28164934389828744 1102 58-59 0.2044448311730278 1102 60-61 0.3109022966892354 1102 62-63 0.19266639423986476 1102 64-65 0.3355394644898695 1102 66-67 0.28524196466081264 1102 68-69 0.20860763368495583 1102 70-71 0.33235657796076623 1102 72-73 0.25974220586621755 1102 74-75 0.13648632090845325 1102 76-77 0.07689932289679291 1102 78-79 0.06426565674589568 1102 80-81 0.2664535038177682 1102 82-83 0.2427943060840434 1102 84-85 0.49822183542836385 1102 86-87 0.546569061866748 1102 88-89 0.4866107087520426 1102 90-91 0.4840310139999353 1102 92-93 0.5827672288057535 1102 94-95 0.6333721254851916 1102 96-97 0.6452241690121845 1102 98-99 0.8316824032695713 1102 100-101 1.5298611245004103 1103 1 0.2087055288291353 1103 2 0.172968577273501 1103 3 0.10603149969170289 1103 4 0.06328674424306513 1103 5 -0.012157469898362194 1103 6 0.07067669996693837 1103 7 0.07422699779129971 1103 8 0.04937693985729652 1103 9 0.15214933969802047 1103 10-11 0.18689507724349141 1103 12-13 0.18650648866545794 1103 14-15 0.219141163601968 1103 16-17 0.13555953487090022 1103 18-19 0.06774001734654433 1103 20-21 0.12530510980909781 1103 22-23 0.2086128110596377 1103 24-25 0.16212721543619324 1103 26-27 0.18233219350906893 1103 28-29 0.16316729393124518 1103 30-31 0.2027061981869238 1103 32-33 0.274134173640725 1103 34-35 0.13461249573774126 1103 36-37 0.23156893246331123 1103 38-39 0.17657474229310566 1103 40-41 0.18617915132751506 1103 42-43 0.22188273430969474 1103 44-45 0.2071646025039513 1103 46-47 0.15428617550033152 1103 48-49 0.1579880284187425 1103 50-51 0.1856150657939324 1103 52-53 0.18836919426919252 1103 54-55 0.2206856854055701 1103 56-57 0.016852094019306207 1103 58-59 0.07545030664598329 1103 60-61 0.13574473141702015 1103 62-63 0.15124915119116622 1103 64-65 0.10922720866255986 1103 66-67 0.13696315462857456 1103 68-69 0.1349683393792276 1103 70-71 0.14563739579151758 1103 72-73 0.20799354471130016 1103 74-75 0.30390192769518976 1103 76-77 0.28547464911714115 1103 78-79 0.2080064668456849 1103 80-81 0.29009700136525396 1103 82-83 0.2882210664729925 1103 84-85 0.3457577975391395 1103 86-87 0.3796646743578833 1103 88-89 0.24921015107193156 1103 90-91 0.2608401530484272 1103 92-93 0.27571873015116566 1103 94-95 0.32738655911713366 1103 96-97 0.3879126299877136 1103 98-99 0.5542570764862909 1103 100-101 1.278487640200641 1104 1 0.14027598583617618 1104 2 0.1265225237002312 1104 3 0.060241812489508106 1104 4 0.04130421162567899 1104 5 -0.10982306367073136 1104 6 0.023874715941708757 1104 7 0.020689125643052364 1104 8 -0.013735652809010901 1104 9 0.12010029018541957 1104 10-11 0.11571759807308268 1104 12-13 0.01271770468462563 1104 14-15 0.04517441439515579 1104 16-17 0.12103097049533318 1104 18-19 0.16050247778277082 1104 20-21 0.15716279886365925 1104 22-23 0.11535185663021252 1104 24-25 0.04924224603472993 1104 26-27 0.04952373001872701 1104 28-29 0.08193956350455522 1104 30-31 -0.048687862749908106 1104 32-33 -0.04119293792624745 1104 34-35 0.07503959314404796 1104 36-37 0.16377183578418197 1104 38-39 0.15498402021355417 1104 40-41 0.19634087005061218 1104 42-43 0.20174635171124322 1104 44-45 0.23575720374320497 1104 46-47 0.1357476527265291 1104 48-49 0.2791105871377084 1104 50-51 0.11040370816802891 1104 52-53 0.13624738951043724 1104 54-55 0.1255403088655953 1104 56-57 0.1136163800343084 1104 58-59 0.3178968309659851 1104 60-61 0.3758533093409113 1104 62-63 0.30095952713791974 1104 64-65 0.16953254340941726 1104 66-67 0.17936289131658611 1104 68-69 0.26176999809167256 1104 70-71 0.14593017729232116 1104 72-73 0.1952092309107627 1104 74-75 0.21284018332979926 1104 76-77 0.2076765764996722 1104 78-79 0.1355315637697565 1104 80-81 0.12589441952995273 1104 82-83 0.2943292922936429 1104 84-85 0.3331213096924408 1104 86-87 0.453934060129086 1104 88-89 0.41707632887641566 1104 90-91 0.26114345937441996 1104 92-93 0.3447687594293143 1104 94-95 0.4320736464961463 1104 96-97 0.42515998634298313 1104 98-99 0.8225774483379311 1104 100-101 1.5306318341329863 1105 1 0.06990113898694261 1105 2 0.12719920525865547 1105 3 0.1389672951566041 1105 4 0.0617651892683071 1105 5 0.028144552560043223 1105 6 0.030266043539562304 1105 7 0.11608332990372361 1105 8 0.1678357392266605 1105 9 0.1589894178372404 1105 10-11 0.1313591967451515 1105 12-13 0.13079326446433015 1105 14-15 0.14232234024587598 1105 16-17 0.1412920507199118 1105 18-19 0.14660225352566414 1105 20-21 0.14201240991884134 1105 22-23 0.09195727120996366 1105 24-25 0.10336657240033986 1105 26-27 0.18880928829775456 1105 28-29 0.16292664001053225 1105 30-31 0.11937335951122208 1105 32-33 0.21142482171493526 1105 34-35 0.20023890931133792 1105 36-37 0.21456603567666122 1105 38-39 0.3146173355339812 1105 40-41 0.19053722219134528 1105 42-43 0.23699528203372466 1105 44-45 0.33735005978394383 1105 46-47 0.1972090192787519 1105 48-49 0.16392232882564173 1105 50-51 0.17729816641766405 1105 52-53 0.19026013334512726 1105 54-55 0.3049903117894672 1105 56-57 0.3480188507375104 1105 58-59 0.4058092636025421 1105 60-61 0.41997848468488996 1105 62-63 0.3188715440921257 1105 64-65 0.33754300310478413 1105 66-67 0.36281971988564976 1105 68-69 0.3831722672946114 1105 70-71 0.4563568294359399 1105 72-73 0.4471486885368847 1105 74-75 0.36276314118454067 1105 76-77 0.4175293580135033 1105 78-79 0.2668968055383658 1105 80-81 0.3793506774164719 1105 82-83 0.47848429108534773 1105 84-85 0.4831395222160353 1105 86-87 0.6123789466372322 1105 88-89 0.5159841763252153 1105 90-91 0.5084503308855943 1105 92-93 0.5340308915416223 1105 94-95 0.6394685123258554 1105 96-97 0.7433383746649866 1105 98-99 0.8757993212617237 1105 100-101 1.7103180009991554 1106 1 0.1534964381115742 1106 2 0.08765624509837977 1106 3 0.04215417117638509 1106 4 0.0735319894646409 1106 5 0.021501168692608985 1106 6 0.007746177871851501 1106 7 0.02257939552911381 1106 8 0.08479898032402389 1106 9 0.06243248010844127 1106 10-11 0.09520652839847799 1106 12-13 0.06473892032548179 1106 14-15 0.13706482830178146 1106 16-17 0.1795783296806448 1106 18-19 0.07029142129592714 1106 20-21 0.07801763997368028 1106 22-23 0.0771266460283826 1106 24-25 0.11814955496533486 1106 26-27 0.16345031370131125 1106 28-29 0.25650279290869804 1106 30-31 0.15424600554921852 1106 32-33 0.09835349190283438 1106 34-35 0.11952708608774287 1106 36-37 0.1256001034060077 1106 38-39 0.1891937398507011 1106 40-41 0.31951166770708284 1106 42-43 0.20056421822452108 1106 44-45 0.16360251351589739 1106 46-47 0.18969553168486897 1106 48-49 0.15663288843945367 1106 50-51 0.2024851611152414 1106 52-53 0.2163404013785808 1106 54-55 0.13991984427785553 1106 56-57 0.0764299883396049 1106 58-59 0.11508696860727952 1106 60-61 0.2253696120450286 1106 62-63 0.22968710405407222 1106 64-65 0.2917310257715542 1106 66-67 0.12757083780644507 1106 68-69 0.10341074105704706 1106 70-71 0.13716476109124898 1106 72-73 0.10702307529682287 1106 74-75 0.1928826044453551 1106 76-77 0.2880225774298779 1106 78-79 0.09391625737730536 1106 80-81 0.056242121879549245 1106 82-83 0.2606017944475987 1106 84-85 0.23880443169678145 1106 86-87 0.3203961116991749 1106 88-89 0.3562980276356633 1106 90-91 0.29600850787122823 1106 92-93 0.5757747416481394 1106 94-95 0.6265025004793436 1106 96-97 0.7140468090545191 1106 98-99 0.9442204984562395 1106 100-101 1.5083714794075114 1107 1 0.08594563658307663 1107 2 0.07382408527031004 1107 3 0.062113617156235534 1107 4 0.052994124386565034 1107 5 0.012436645650339528 1107 6 0.027776672786544054 1107 7 0.06520516292434309 1107 8 0.06462200687312247 1107 9 0.053428430798291515 1107 10-11 0.04726518155297299 1107 12-13 0.05359631861068692 1107 14-15 0.12592292817810602 1107 16-17 0.07388314836587995 1107 18-19 0.0871253130615699 1107 20-21 0.03215209590756274 1107 22-23 0.09256909437193883 1107 24-25 0.045243611092210756 1107 26-27 0.021145799746186356 1107 28-29 0.043378798622448755 1107 30-31 0.10798267643022541 1107 32-33 0.0735907477739417 1107 34-35 0.11206220791031285 1107 36-37 0.11487292919395742 1107 38-39 0.1250528080596638 1107 40-41 -0.023389605964858617 1107 42-43 -0.06759951384049856 1107 44-45 -0.004287693839529538 1107 46-47 -0.0494918882182418 1107 48-49 1.337658390383467E-4 1107 50-51 -0.15179716969229418 1107 52-53 -0.18426994182679124 1107 54-55 -0.06525519107035649 1107 56-57 -0.04081757523977814 1107 58-59 -0.0052808615849428975 1107 60-61 -0.037551796949813365 1107 62-63 0.007507159826808163 1107 64-65 -0.06189281899021637 1107 66-67 -0.030207456524919962 1107 68-69 0.0022234709179755896 1107 70-71 -0.04595635632916384 1107 72-73 -0.07973384131355488 1107 74-75 0.01619471824083618 1107 76-77 0.05815088441384475 1107 78-79 0.1051700013926471 1107 80-81 0.1653135650059383 1107 82-83 0.1386634953139243 1107 84-85 0.15523782787214202 1107 86-87 0.13197994920273004 1107 88-89 0.19204883862365918 1107 90-91 0.3670089815992412 1107 92-93 0.5305234833872454 1107 94-95 0.6281785332163388 1107 96-97 0.8371924780572435 1107 98-99 0.8961096916873714 1107 100-101 1.511551398377506 1108 1 0.17343200576509332 1108 2 0.03097757257049949 1108 3 0.02406115115414309 1108 4 -0.0010050673791468512 1108 5 -0.04043084800685648 1108 6 0.034928044832291505 1108 7 0.028237837244979858 1108 8 0.04879409776220456 1108 9 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1870.0 9 3351.0 10 5465.0 11 8534.0 12 11921.0 13 16556.0 14 19868.0 15 25831.0 16 34192.0 17 45935.0 18 44191.0 19 30417.0 20 28010.0 21 29785.0 22 32318.0 23 35922.0 24 42049.0 25 50224.0 26 61426.0 27 74355.0 28 91038.0 29 111448.0 30 135618.0 31 163346.0 32 202266.0 33 256212.0 34 339056.0 35 475601.0 36 728793.0 37 1139946.0 38 941741.0 39 85558.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9580443980898 19.688843159285483 14.908431410409422 22.444681032215296 2 15.461298074279576 21.702190026813636 38.94122349209792 23.895288406808866 3 20.117830594393595 25.10711613668243 30.098146025298327 24.676907243625646 4 12.995000930950052 17.200866409236543 37.56796788582571 32.2361647739877 5 13.400469515800925 38.25313418297309 33.91859070914031 14.427805592085674 6 30.965492919023358 36.668158263477935 17.441216232871128 14.92513258462758 7 27.928482559001566 33.28252608650972 20.926524719852292 17.862466634636423 8 26.02090712834711 34.12445759361356 19.807393829033582 20.047241449005742 9 26.07924792887314 17.613897283028912 20.997720594397386 35.30913419370056 10-11 25.050927328635986 26.459363745885145 27.25277588069581 21.236933044783058 12-13 26.60625856293924 24.082227348240924 27.475246398398838 21.836267690421 14-15 22.976937466321793 25.477097680739874 26.849784478427175 24.696180374511158 16-17 21.796601733499873 28.763450977789677 27.14210480160978 22.297842487100667 18-19 21.58437080859174 27.92844463842924 29.098957904751867 21.388226648227157 20-21 23.01089533884117 26.23799292477961 28.69071450321585 22.06039723316336 22-23 22.34527553277456 26.40849329810765 28.81420284700073 22.43202832211706 24-25 22.760156020994426 26.5451933406672 28.846118113171343 21.84853252516703 26-27 22.45315961929869 26.21555851571882 29.547164226902044 21.784117638080446 28-29 21.650390525014114 27.517726919548853 29.033895682780763 21.79798687265627 30-31 22.18903329464171 26.446849957017033 28.865035374205895 22.49908137413536 32-33 22.128578422208413 26.698130629545965 28.98149893196708 22.191792016278544 34-35 22.89702551788163 26.517693530575343 28.748119000521825 21.8371619510212 36-37 22.685564721961313 26.12858012747758 29.255897843470457 21.92995730709065 38-39 22.208297102263444 26.868136541142423 29.137564477556268 21.786001879037862 40-41 22.32389448089472 26.867716568579286 28.785838933924744 22.02255001660125 42-43 22.052652122687014 27.04907922355123 28.07934651926052 22.818922134501236 44-45 22.454436118225193 26.476397566993665 28.21325836386221 22.855907950918937 46-47 22.910196231638864 27.092880093747816 27.952971374815977 22.043952299797343 48-49 22.796864145059175 26.994341779140917 27.867003179312626 22.341790896487286 50-51 21.91321567076424 27.27003146272272 28.383190707632544 22.433562158880495 52-53 22.577984605006048 27.6840275894916 27.315799871904307 22.422187933598046 54-55 22.01555615638596 26.888198018195048 27.78641313477616 23.309832690642835 56-57 22.596835923831385 27.057689020592935 27.51958646328213 22.82588859229355 58-59 23.3315061854423 26.70859500227335 27.369013215000386 22.590885597283965 60-61 22.5548413226005 26.973072225169574 28.3504334632655 22.121652988964424 62-63 22.210871216839788 26.999405968265094 28.16149358500712 22.628229229888007 64-65 21.940286650018002 27.640636871107983 27.677553143220536 22.741523335653476 66-67 22.28213893926016 27.105111791675014 27.19877123063421 23.413978038430614 68-69 21.941951608106567 27.0585875290105 27.65889068580194 23.340570177080995 70-71 22.550268013277517 26.879254572133405 27.489084083039273 23.0813933315498 72-73 22.346327828656655 26.49101794363562 28.049183740720363 23.113470486987367 74-75 21.79518925962955 27.72809948585148 27.315948311739678 23.160762942779293 76-77 21.994373210799512 26.79587849406032 27.4935878336596 23.716160461480566 78-79 22.025872593870798 26.960485727750484 27.608183488036236 23.405458190342483 80-81 22.303420509862676 26.816471582210966 27.482269100131475 23.397838807794884 82-83 22.025608566791114 26.66042677910978 27.9176949161412 23.396269737957905 84-85 21.724506283628436 26.929161716451954 27.66798718739702 23.678344812522585 86-87 21.704509553322875 26.884407398265125 27.599688301897324 23.811394746514676 88-89 21.566818996238442 26.88218611710298 27.18751760137249 24.363477285286088 90-91 21.735653080681853 26.36482082644354 27.482882245438983 24.41664384743562 92-93 22.038850984954546 26.716076821449214 27.471515800075537 23.773556393520703 94-95 21.686386056108205 26.391139376348793 27.609218215733065 24.313256351809937 96-97 21.03636113704987 26.343219555665105 28.15247882370229 24.46794048358274 98-99 21.275517227126407 26.62548429310934 27.248566697167448 24.850431782596807 100-101 21.168101188540636 25.665945850630866 28.042297143691407 25.12365581713709 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1023.0 1 724.0 2 549.5 3 717.0 4 670.0 5 675.0 6 1167.5 7 2458.5 8 3996.5 9 4709.0 10 4398.0 11 4068.5 12 4317.5 13 4698.5 14 5187.0 15 5743.5 16 6176.0 17 6668.5 18 7168.5 19 7778.0 20 8679.0 21 10025.5 22 11851.5 23 13960.5 24 16499.0 25 20425.5 26 26698.0 27 34083.5 28 40876.0 29 48556.5 30 58029.5 31 68860.5 32 79257.5 33 88740.0 34 99947.5 35 109542.0 36 120105.0 37 132428.5 38 139252.5 39 142373.0 40 146338.0 41 153326.0 42 160497.0 43 167711.0 44 175665.0 45 182608.5 46 188037.5 47 217015.0 48 279261.0 49 279253.5 50 224219.0 51 195112.5 52 181297.0 53 175963.0 54 170360.0 55 157628.0 56 143244.0 57 128743.0 58 116183.0 59 103413.0 60 87573.5 61 71153.0 62 56821.0 63 44471.5 64 33290.5 65 25036.5 66 18114.0 67 13907.5 68 10265.5 69 6681.0 70 4766.0 71 3543.0 72 2769.5 73 2259.5 74 1722.5 75 1297.0 76 909.0 77 457.5 78 235.5 79 140.0 80 90.0 81 62.5 82 48.5 83 39.5 84 23.5 85 20.0 86 14.0 87 5.0 88 2.0 89 4.5 90 4.5 91 1.5 92 1.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2338561695443957 2 0.0 3 0.0 4 0.0 5 0.02874379382433143 6 0.0036403749434509463 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.003213768504765289 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.7920572327614023E-4 26-27 0.0013935810330398153 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007394511603884735 36-37 0.0178416292801424 38-39 0.014002171331971479 40-41 0.009157818217118786 42-43 0.00859848977528648 44-45 0.004038540952890894 46-47 6.16209300323728E-4 48-49 0.0016305846100874034 50-51 7.584114465522806E-5 52-53 0.0 54-55 0.0 56-57 0.0012798193160569733 58-59 4.550468679313683E-4 60-61 0.002806122352243438 62-63 0.0010048951666817717 64-65 0.001611624323923596 66-67 0.0054510822720945165 68-69 2.5596386321139467E-4 70-71 5.6880858491421036E-5 72-73 0.0 74-75 4.7400715409517534E-4 76-77 0.05967750070058257 78-79 0.1427330342411392 80-81 0.001061776025173193 82-83 9.195738789446401E-4 84-85 0.0 86-87 0.10660420895600493 88-89 0.3585390113575906 90-91 0.39698099155470934 92-93 5.688085849142103E-4 94-95 3.0336457862091223E-4 96-97 1.3272200314664911E-4 98-99 0.0 100-101 0.00127033917297507 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5274182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.28920531751306 #Duplication Level Percentage of deduplicated Percentage of total 1 69.1844971673429 29.25757405498664 2 15.275217080947995 12.919535828115844 3 6.01809662648896 7.635015715746729 4 2.9756957974924885 5.033592421704824 5 1.7115867005442347 3.6190820699019945 6 1.0713046244202111 2.7182772731824514 7 0.7198341367911034 2.1308849523719626 8 0.502279280287218 1.699279328863908 9 0.38644998108421275 1.4708396335517377 >10 1.9000607587061074 14.540149229157073 >50 0.1496857043993351 4.399905829329438 >100 0.09694273294499736 7.585884457763657 >500 0.005745831541174183 1.6750698110929978 >1k 0.0019302381275416796 1.6342542444061612 >5k 3.5911407023938473E-4 1.231921955076693 >10k+ 3.1422481145946163E-4 2.448733194747869 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 39152 0.7423331238853722 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19112 0.3623689891626797 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17895 0.3392943209013265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14533 0.2755498388186073 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13756 0.26081769646932923 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13286 0.25190636197234 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 11260 0.213492822204467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9595 0.1819239457417283 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9470 0.17955390997125242 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9118 0.17287988924159234 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8918 0.16908783200883093 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7737 0.14669573404937486 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7297 0.1383532081372998 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6699 0.1270149570113432 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6061 0.1149182944388343 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8960286163807014E-5 2 1.8960286163807014E-5 0.0 0.0 0.0 1.8960286163807014E-5 3 1.8960286163807014E-5 0.0 0.0 0.0 1.8960286163807014E-5 4 1.8960286163807014E-5 0.0 0.0 0.0 1.8960286163807014E-5 5 1.8960286163807014E-5 0.0 0.0 0.0 1.8960286163807014E-5 6 1.8960286163807014E-5 0.0 0.0 0.0 1.8960286163807014E-5 7 5.6880858491421036E-5 0.0 0.0 1.8960286163807014E-5 1.8960286163807014E-5 8 5.6880858491421036E-5 0.0 0.0 1.8960286163807014E-5 1.8960286163807014E-5 9 5.6880858491421036E-5 0.0 0.0 3.792057232761403E-5 1.8960286163807014E-5 10-11 5.6880858491421036E-5 0.0 0.0 3.792057232761403E-5 1.8960286163807014E-5 12-13 6.636100157332454E-5 0.0 0.0 4.740071540951753E-5 1.8960286163807014E-5 14-15 7.584114465522806E-5 0.0 0.0 8.532128773713156E-5 1.8960286163807014E-5 16-17 7.584114465522806E-5 0.0 9.480143081903507E-6 1.6116243239235961E-4 1.8960286163807014E-5 18-19 8.532128773713156E-5 0.0 1.8960286163807014E-5 1.9908300471997364E-4 1.8960286163807014E-5 20-21 9.480143081903507E-5 0.0 1.8960286163807014E-5 3.3180500786662273E-4 1.8960286163807014E-5 22-23 9.480143081903507E-5 0.0 1.8960286163807014E-5 5.972490141599208E-4 1.8960286163807014E-5 24-25 1.5168228931045611E-4 0.0 1.8960286163807014E-5 0.0011091767405827103 1.8960286163807014E-5 26-27 1.5168228931045611E-4 0.0 1.8960286163807014E-5 0.0018770683302168942 1.8960286163807014E-5 28-29 1.6116243239235961E-4 0.0 1.8960286163807014E-5 0.005071876548818376 1.8960286163807014E-5 30-31 1.8960286163807014E-4 0.0 1.8960286163807014E-5 0.01462786077537711 1.8960286163807014E-5 32-33 1.8960286163807014E-4 0.0 1.8960286163807014E-5 0.035853901135759064 1.8960286163807014E-5 34-35 2.0856314780187714E-4 0.0 1.8960286163807014E-5 0.06820962947429574 1.8960286163807014E-5 36-37 2.2752343396568414E-4 9.480143081903507E-6 1.8960286163807014E-5 0.11831218566215576 1.8960286163807014E-5 38-39 3.2232486478471923E-4 1.8960286163807014E-5 1.8960286163807014E-5 0.19210561941169266 1.8960286163807014E-5 40-41 3.2232486478471923E-4 1.8960286163807014E-5 1.8960286163807014E-5 0.27487674865979217 1.8960286163807014E-5 42-43 3.5076529403042973E-4 3.792057232761403E-5 1.8960286163807014E-5 0.36271975445671006 1.8960286163807014E-5 44-45 4.171262956037543E-4 3.792057232761403E-5 1.8960286163807014E-5 0.4723481290558422 1.8960286163807014E-5 46-47 4.929674402589823E-4 3.792057232761403E-5 1.8960286163807014E-5 0.6052786953502932 1.8960286163807014E-5 48-49 5.119277264227893E-4 3.792057232761403E-5 1.8960286163807014E-5 0.7462199825489526 1.8960286163807014E-5 50-51 5.119277264227893E-4 3.792057232761403E-5 1.8960286163807014E-5 0.895608077233588 1.8960286163807014E-5 52-53 5.119277264227893E-4 3.792057232761403E-5 1.8960286163807014E-5 1.0452710960675988 1.8960286163807014E-5 54-55 5.308880125865963E-4 3.792057232761403E-5 1.8960286163807014E-5 1.216965967423953 1.8960286163807014E-5 56-57 5.308880125865963E-4 3.792057232761403E-5 1.8960286163807014E-5 1.4289040461629878 1.8960286163807014E-5 58-59 5.403681556684998E-4 3.792057232761403E-5 1.8960286163807014E-5 1.6543987295091447 1.8960286163807014E-5 60-61 5.498482987504033E-4 3.792057232761403E-5 1.8960286163807014E-5 1.9019916263792185 1.8960286163807014E-5 62-63 5.97249014159921E-4 3.792057232761403E-5 1.8960286163807014E-5 2.1560594609742325 1.8960286163807014E-5 64-65 6.067291572418245E-4 3.792057232761403E-5 1.8960286163807014E-5 2.421569828269104 1.8960286163807014E-5 66-67 7.204908742246665E-4 3.792057232761403E-5 1.8960286163807014E-5 2.7201564147767368 1.8960286163807014E-5 68-69 7.394511603884735E-4 3.792057232761403E-5 1.8960286163807014E-5 3.0580571546450237 1.8960286163807014E-5 70-71 7.394511603884735E-4 3.792057232761403E-5 1.8960286163807014E-5 3.423601991740141 1.8960286163807014E-5 72-73 7.394511603884735E-4 3.792057232761403E-5 1.8960286163807014E-5 3.79408598338093 1.8960286163807014E-5 74-75 7.489313034703771E-4 3.792057232761403E-5 1.8960286163807014E-5 4.183672463331755 1.8960286163807014E-5 76-77 7.584114465522806E-4 3.792057232761403E-5 1.8960286163807014E-5 4.591982225869339 1.8960286163807014E-5 78-79 7.584114465522806E-4 3.792057232761403E-5 1.8960286163807014E-5 5.066927914129622 1.8960286163807014E-5 80-81 7.963320188798946E-4 3.792057232761403E-5 1.8960286163807014E-5 5.582837300646812 1.8960286163807014E-5 82-83 7.963320188798946E-4 3.792057232761403E-5 1.8960286163807014E-5 6.088877858215739 1.8960286163807014E-5 84-85 8.342525912075086E-4 3.792057232761403E-5 1.8960286163807014E-5 6.607517525940516 1.8960286163807014E-5 86-87 8.721731635351226E-4 3.792057232761403E-5 1.8960286163807014E-5 7.1486251327694035 1.8960286163807014E-5 88-89 8.911334496989296E-4 3.792057232761403E-5 1.8960286163807014E-5 7.732668307616233 1.8960286163807014E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 25485 0.0 23.501919 1 GAGTACT 17790 0.0 22.536182 12-13 TACATGG 26220 0.0 22.428003 2 ACATGGG 25955 0.0 21.358648 3 GTACTTT 19630 0.0 20.290838 14-15 AGTACTT 19010 0.0 20.253777 12-13 CATGGGG 10990 0.0 19.304644 4 GGACCGT 500 0.0 18.9784 6 AGAGTAC 35285 0.0 18.468777 10-11 ACTTTTT 23100 0.0 17.540226 16-17 CATGGGA 14130 0.0 17.533972 4 TACTTTT 22645 0.0 16.824524 14-15 AGTACAT 19960 0.0 15.93185 2 GTACCGT 420 8.785719E-10 15.815333 6 GAGTACA 23750 0.0 15.303543 1 ACCGTAT 410 1.0877557E-8 15.04314 8 TACCTGG 4445 0.0 14.948866 2 ATGGGGA 5800 0.0 14.729787 5 ATGGGAT 5925 0.0 14.659349 5 TACACTG 3895 0.0 14.500783 5 >>END_MODULE