FastQCFastQC Report
Thu 26 May 2016
SRR938735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197768
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204150.4863298781638242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151790.36159692484196365No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT108130.2575892712508171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106290.25320598946868905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60180.14336190089590467No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47640.11348888266335824No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43580.10381707612235837No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42850.10207805671966627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG237000.026.5440081
TACATGG240000.025.6660142
ACATGGG243400.024.295193
GTATCAA377300.022.5036351
GAGTACT170650.022.10185812-13
CATGGGG94350.021.8460874
AGTACTT178950.020.6533512-13
GTACTTT182800.020.58114614-15
CATGGGA147500.020.4310844
TATCAAC435650.019.3927752
ATCAACG435250.019.367053
CAACGCA434750.019.3566275
TCAACGC435650.019.3492684
GGTATCA286300.019.0589031
AACGCAG442900.019.0180176
AGAGTAC339700.017.82878310-11
TACCTGG29150.017.717982
ACTTTTT217250.017.6891316-17
ACGCAGA478350.017.5016127
TACTTTT210200.017.4590614-15
Produced by FastQC (version 0.11.5)