##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938735_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4197768 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.563933261676205 33.0 31.0 34.0 30.0 34.0 2 31.60772081734865 33.0 31.0 34.0 28.0 34.0 3 31.61810228673905 33.0 31.0 34.0 28.0 34.0 4 35.17045725252086 37.0 35.0 37.0 33.0 37.0 5 35.21341889308795 37.0 35.0 37.0 33.0 37.0 6 35.3077580752438 37.0 35.0 37.0 33.0 37.0 7 35.218852256723096 37.0 35.0 37.0 32.0 37.0 8 35.19157085384423 37.0 35.0 37.0 32.0 37.0 9 36.74245479978884 39.0 37.0 39.0 32.0 39.0 10-11 36.63820320703765 39.0 37.0 39.0 32.0 39.0 12-13 36.51686741620785 39.0 37.0 39.0 32.0 39.0 14-15 37.64843018956741 40.0 37.0 41.0 32.0 41.0 16-17 37.59080218344606 40.0 37.0 41.0 32.0 41.0 18-19 37.548375112678926 40.0 37.0 41.0 32.0 41.0 20-21 37.44942562333125 40.0 37.0 41.0 32.0 41.0 22-23 37.36168244648108 40.0 37.0 41.0 31.5 41.0 24-25 37.367421686953634 40.0 37.0 41.0 31.0 41.0 26-27 37.27007292923287 40.0 37.0 41.0 31.0 41.0 28-29 37.15249449231115 40.0 37.0 41.0 31.0 41.0 30-31 36.94643582017872 39.5 36.0 41.0 30.0 41.0 32-33 36.72794613709 39.0 36.0 41.0 30.0 41.0 34-35 36.395302694193674 39.0 35.0 41.0 30.0 41.0 36-37 36.14124577632685 39.0 35.0 41.0 29.0 41.0 38-39 35.968392488579646 39.0 35.0 41.0 28.5 41.0 40-41 35.78716963395786 39.0 35.0 41.0 27.5 41.0 42-43 35.514920071809584 39.0 35.0 40.0 26.5 41.0 44-45 35.446180684592385 39.0 35.0 41.0 26.0 41.0 46-47 35.23051845647497 39.0 35.0 41.0 24.5 41.0 48-49 34.98406712805472 39.0 35.0 41.0 24.0 41.0 50-51 33.826587486492826 38.0 33.0 40.0 23.0 40.5 52-53 33.92226261670488 38.0 33.5 39.5 23.0 40.5 54-55 34.43868312874842 38.0 34.0 40.0 23.0 41.0 56-57 34.34386976602804 38.0 34.0 40.0 23.0 41.0 58-59 34.126500440233954 38.0 34.0 40.0 22.0 41.0 60-61 33.83181061935771 38.0 33.5 40.0 21.5 41.0 62-63 33.47067262888278 37.0 33.0 40.0 20.0 41.0 64-65 33.07687228069774 36.5 33.0 39.5 18.5 41.0 66-67 32.67876631104911 36.0 32.0 39.0 17.5 41.0 68-69 32.277899350321405 35.5 32.0 39.0 13.5 40.5 70-71 31.873970167003037 35.0 31.0 38.5 12.0 40.0 72-73 31.46408091157015 35.0 31.0 37.5 8.0 40.0 74-75 31.02196131372672 35.0 31.0 37.0 7.0 39.0 76-77 30.593261466569853 35.0 30.5 36.5 4.0 39.0 78-79 30.18647088166854 34.5 30.0 36.0 2.0 38.5 80-81 29.76384295177818 34.0 30.0 35.5 2.0 37.0 82-83 29.317408560930474 34.0 29.0 35.0 2.0 37.0 84-85 28.927803061055304 34.0 29.0 35.0 2.0 36.5 86-87 28.57295865326526 34.0 29.0 35.0 2.0 36.0 88-89 28.21310491670812 34.0 28.5 35.0 2.0 36.0 90-91 27.817051347287418 34.0 27.0 35.0 2.0 35.0 92-93 27.38880614650452 34.0 26.5 35.0 2.0 35.0 94-95 26.908379405436413 33.5 25.5 35.0 2.0 35.0 96-97 26.345748026093865 33.0 25.0 35.0 2.0 35.0 98-99 25.676681989095158 33.0 22.0 35.0 2.0 35.0 100-101 23.47919322840138 31.0 9.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32774248347079293 1101 2 0.1772367019825225 1101 3 0.321555105396115 1101 4 0.2678544961598064 1101 5 0.13212434233698644 1101 6 0.0817511115926095 1101 7 0.1762943871815068 1101 8 0.3555977548572784 1101 9 0.4492145449963516 1101 10-11 0.4542904113002919 1101 12-13 0.39329719693916587 1101 14-15 0.5270680874380034 1101 16-17 0.5335605514781605 1101 18-19 0.49274626162452506 1101 20-21 0.5428239669924011 1101 22-23 0.4740294022330218 1101 24-25 0.5323403264280344 1101 26-27 0.4615480121894322 1101 28-29 0.3930209235698712 1101 30-31 0.40004673937884405 1101 32-33 0.388823604471888 1101 34-35 0.49650463212501705 1101 36-37 0.6136735426728492 1101 38-39 0.5900504649276854 1101 40-41 0.5068761909971258 1101 42-43 0.5248516284649369 1101 44-45 0.5674793033271968 1101 46-47 0.5700453900275662 1101 48-49 0.6617615607964851 1101 50-51 0.6254347808453176 1101 52-53 0.5561710597788334 1101 54-55 0.5598055167654508 1101 56-57 0.48358354627417555 1101 58-59 0.49851165906170536 1101 60-61 0.4328548377877155 1101 62-63 0.43710458120140316 1101 64-65 0.4968786474160538 1101 66-67 0.31048572011104625 1101 68-69 0.2745213684141774 1101 70-71 0.14472391741828616 1101 72-73 0.18475638891093027 1101 74-75 0.16764954737367077 1101 76-77 0.13245693591228758 1101 78-79 0.05086329747365781 1101 80-81 -0.05878086844610664 1101 82-83 -0.153650624879468 1101 84-85 -0.14067831504086215 1101 86-87 -0.24080098600901678 1101 88-89 -0.23357003535890897 1101 90-91 -0.26429867048912925 1101 92-93 -0.3344068209152731 1101 94-95 -0.4492026695915676 1101 96-97 -0.6401433743104654 1101 98-99 -0.6025647837074928 1101 100-101 -0.4459825727040325 1102 1 0.27844098040065646 1102 2 0.22175345044777117 1102 3 0.3052402168636341 1102 4 0.22755431740769438 1102 5 0.21666047941189248 1102 6 0.2621662633406885 1102 7 0.20495448102730762 1102 8 0.1136890587035353 1102 9 0.32901296484730835 1102 10-11 0.29192137279140695 1102 12-13 0.28712257515984874 1102 14-15 0.24674706077262698 1102 16-17 0.31817756039136214 1102 18-19 0.3505640152354488 1102 20-21 0.36228556070038564 1102 22-23 0.379087424018671 1102 24-25 0.4441584149239759 1102 26-27 0.4305941383862333 1102 28-29 0.4341975336503623 1102 30-31 0.49053963426860037 1102 32-33 0.4270972067892558 1102 34-35 0.5058799029925254 1102 36-37 0.40061345471458054 1102 38-39 0.3434881313401874 1102 40-41 0.39701563248892313 1102 42-43 0.350778943571477 1102 44-45 0.47660648629129554 1102 46-47 0.43272174403801245 1102 48-49 0.4328570839172059 1102 50-51 0.3130630124322735 1102 52-53 0.315231227998801 1102 54-55 0.2343345799919092 1102 56-57 0.2677243001328051 1102 58-59 0.22568003640540724 1102 60-61 0.21665916555384257 1102 62-63 0.13796762719324107 1102 64-65 0.20126971713214203 1102 66-67 0.29206377329184363 1102 68-69 0.174230098962191 1102 70-71 0.10390571733593035 1102 72-73 0.1585844391668303 1102 74-75 0.12127728179098085 1102 76-77 0.02919290146869713 1102 78-79 -0.09416666668408169 1102 80-81 -0.16417614661767743 1102 82-83 -0.11786579567396771 1102 84-85 -0.2503217077858828 1102 86-87 -0.354303136028701 1102 88-89 -0.35259555518235075 1102 90-91 -0.35421171234837345 1102 92-93 -0.40413413997200465 1102 94-95 -0.38947950577605894 1102 96-97 -0.5224708553750581 1102 98-99 -0.5929228439759768 1102 100-101 -0.5130587711225232 1103 1 0.11302802083044128 1103 2 0.20416564213096322 1103 3 0.13217755805723996 1103 4 0.14774914090019564 1103 5 0.07264305040997243 1103 6 0.1550725317389876 1103 7 0.1402489845830246 1103 8 0.10728245526247093 1103 9 0.2880438197679851 1103 10-11 0.18553361550567615 1103 12-13 0.2583246935790626 1103 14-15 0.3392771444171174 1103 16-17 0.3486830343703531 1103 18-19 0.4191121543924581 1103 20-21 0.2746387737217333 1103 22-23 0.35773873649542764 1103 24-25 0.20623932097256414 1103 26-27 0.202568549737407 1103 28-29 0.24674262159424387 1103 30-31 0.3751640644054106 1103 32-33 0.2818475757236314 1103 34-35 0.3227659613403162 1103 36-37 0.22232247056036414 1103 38-39 0.20205711625745693 1103 40-41 0.27869524494321496 1103 42-43 0.29389084930632947 1103 44-45 0.3329086067440059 1103 46-47 0.28555499780676286 1103 48-49 0.3342873340179082 1103 50-51 0.39834017074675643 1103 52-53 0.24942818811515366 1103 54-55 0.30677420209079287 1103 56-57 0.3033767595984429 1103 58-59 0.2224688669899706 1103 60-61 0.23984810691555225 1103 62-63 0.16833892671372297 1103 64-65 -0.0683827558901342 1103 66-67 -0.04153012195790495 1103 68-69 0.005081678892160824 1103 70-71 -0.09484339179892132 1103 72-73 0.06949041997062366 1103 74-75 -0.007222571506769526 1103 76-77 -0.05966835499975787 1103 78-79 0.008687648298717932 1103 80-81 -0.03829932228079258 1103 82-83 -0.01340973904980558 1103 84-85 0.09357402613206389 1103 86-87 0.08326815739129145 1103 88-89 -0.11286241286858001 1103 90-91 -0.11794011371023316 1103 92-93 -0.05379972662607102 1103 94-95 -0.10979705320494304 1103 96-97 -0.11939510714516999 1103 98-99 -0.08108220522566612 1103 100-101 -0.19133795373438645 1104 1 0.17791657189358645 1104 2 0.2297998084908066 1104 3 0.19847114620770512 1104 4 0.2605199368393798 1104 5 0.1933426037896382 1104 6 0.18665104889810635 1104 7 0.2665435171753785 1104 8 0.18682136147411654 1104 9 0.241445919976627 1104 10-11 0.19012093035671285 1104 12-13 0.2115162719380166 1104 14-15 0.22659617832497503 1104 16-17 0.309519258558808 1104 18-19 0.2823102863151661 1104 20-21 0.3187916117456382 1104 22-23 0.42136667337410927 1104 24-25 0.38887999966672027 1104 26-27 0.30242212528892765 1104 28-29 0.37834526516858347 1104 30-31 0.33563467692151505 1104 32-33 0.22928614864797225 1104 34-35 0.3149270936734041 1104 36-37 0.47067143512605725 1104 38-39 0.3839728039645607 1104 40-41 0.3239178911255607 1104 42-43 0.4181236101257042 1104 44-45 0.5992708227402588 1104 46-47 0.604235039592318 1104 48-49 0.5813848531560382 1104 50-51 0.6302190515879147 1104 52-53 0.671272805371693 1104 54-55 0.6310577326542699 1104 56-57 0.6781052325557653 1104 58-59 0.6314035149672321 1104 60-61 0.5679754440496652 1104 62-63 0.4862622164589041 1104 64-65 0.41757903831933874 1104 66-67 0.39782879855940223 1104 68-69 0.44957462386332026 1104 70-71 0.42021110777152515 1104 72-73 0.2960210390642679 1104 74-75 0.30856481736550023 1104 76-77 0.2517528478644415 1104 78-79 0.3127238069994718 1104 80-81 0.25348288270816255 1104 82-83 0.32143092593316425 1104 84-85 0.44874506120202184 1104 86-87 0.46005336829427534 1104 88-89 0.46207845164571637 1104 90-91 0.31294483792667194 1104 92-93 0.39155999546830955 1104 94-95 0.5004169779415051 1104 96-97 0.5952581364613927 1104 98-99 0.7309562407503414 1104 100-101 0.8979613039438767 1105 1 0.1399379494155646 1105 2 0.15621366743771148 1105 3 0.1916385914161367 1105 4 0.2584462148074067 1105 5 0.1777777920702519 1105 6 0.17842633494067428 1105 7 0.1709573219104783 1105 8 0.1958030529426864 1105 9 0.23383705212994954 1105 10-11 0.3169052065236997 1105 12-13 0.34458071471595275 1105 14-15 0.44146557305017353 1105 16-17 0.3561608070889122 1105 18-19 0.2844278596742811 1105 20-21 0.2777336081254873 1105 22-23 0.38288234510356034 1105 24-25 0.3597266675983306 1105 26-27 0.28816110749239243 1105 28-29 0.36213171884309503 1105 30-31 0.23003414564582414 1105 32-33 0.39800105779801953 1105 34-35 0.3935172554354054 1105 36-37 0.4435187596228687 1105 38-39 0.47970479886866 1105 40-41 0.445476932299421 1105 42-43 0.3366583854930596 1105 44-45 0.4091869450300507 1105 46-47 0.5611975220111347 1105 48-49 0.45890731340824686 1105 50-51 0.528258361170451 1105 52-53 0.5356579079675328 1105 54-55 0.5753269424569751 1105 56-57 0.42496091771769073 1105 58-59 0.4892451622832965 1105 60-61 0.536212927899065 1105 62-63 0.48098933531226606 1105 64-65 0.39462868127436224 1105 66-67 0.3813634986325809 1105 68-69 0.381290819037865 1105 70-71 0.36528457982684515 1105 72-73 0.4404898687819738 1105 74-75 0.32467228925818503 1105 76-77 0.3181657731635852 1105 78-79 0.2961556853481824 1105 80-81 0.2759297325504946 1105 82-83 0.27488873559551763 1105 84-85 0.20324834553390403 1105 86-87 0.2545172587141451 1105 88-89 0.3063232059005898 1105 90-91 0.34002342396231455 1105 92-93 0.34804975094294477 1105 94-95 0.4000663179127706 1105 96-97 0.4407883128605796 1105 98-99 0.5569840212889048 1105 100-101 0.7909923161084045 1106 1 0.20254818668006536 1106 2 0.1491793396176071 1106 3 0.19213868527312172 1106 4 0.2736772072779914 1106 5 0.23322343397754253 1106 6 0.13621607741498565 1106 7 0.20107367477699256 1106 8 0.16215338680539304 1106 9 0.2821768561327218 1106 10-11 0.29403562601973476 1106 12-13 0.17374419570767685 1106 14-15 0.2651057641012784 1106 16-17 0.17676783831796428 1106 18-19 0.24362182183948988 1106 20-21 0.27624667510362144 1106 22-23 0.19814179262856157 1106 24-25 0.17962977143395165 1106 26-27 0.11118839752835186 1106 28-29 0.2163518566787772 1106 30-31 0.23105107863789698 1106 32-33 0.3068925621020355 1106 34-35 0.26495526364119115 1106 36-37 0.4531933589429187 1106 38-39 0.3544066763063256 1106 40-41 0.4425521996428543 1106 42-43 0.35189855509114665 1106 44-45 0.31077172768409156 1106 46-47 0.38589436394703114 1106 48-49 0.5716172774446306 1106 50-51 0.4921540219981253 1106 52-53 0.34192576686621834 1106 54-55 0.4052124965260475 1106 56-57 0.4575429084988514 1106 58-59 0.47574721670843445 1106 60-61 0.5626283955303251 1106 62-63 0.3489558566744151 1106 64-65 0.2429799566865114 1106 66-67 0.11535471481055026 1106 68-69 0.33294368525143625 1106 70-71 0.2781939974070049 1106 72-73 0.25795915669051084 1106 74-75 0.08262254207612685 1106 76-77 0.07650697757217984 1106 78-79 0.367686358705015 1106 80-81 0.24916529570730717 1106 82-83 0.26205386727835744 1106 84-85 0.23212021436057384 1106 86-87 0.4257832255751808 1106 88-89 0.4287089610917789 1106 90-91 0.4395088195173642 1106 92-93 0.4185423064452003 1106 94-95 0.44253127052244423 1106 96-97 0.46523400542085014 1106 98-99 0.40938777318466535 1106 100-101 0.5020070740020763 1107 1 0.17526666034597227 1107 2 0.14710341949782801 1107 3 0.1638577078353478 1107 4 0.20773579261987152 1107 5 0.16720822006603697 1107 6 0.17792392088469455 1107 7 -0.13335603344582125 1107 8 0.023427113638952335 1107 9 0.11980648618810363 1107 10-11 0.07770311555189835 1107 12-13 0.2224933730816545 1107 14-15 0.11085897342044149 1107 16-17 0.132764429987958 1107 18-19 0.2924868134623537 1107 20-21 0.2211546703101206 1107 22-23 0.25701891169882884 1107 24-25 0.11638909907907191 1107 26-27 0.16344916063792425 1107 28-29 -0.034814591099909364 1107 30-31 0.20281884089394708 1107 32-33 0.23650543214004927 1107 34-35 0.23338656969830396 1107 36-37 0.3968915406879532 1107 38-39 0.3840413872448849 1107 40-41 0.29073848528160084 1107 42-43 0.3042563090419037 1107 44-45 0.24380051456575558 1107 46-47 0.3755816654750319 1107 48-49 0.3783097891769316 1107 50-51 0.5153168430378088 1107 52-53 0.45488541005487804 1107 54-55 0.3656024715527053 1107 56-57 0.35963765202632914 1107 58-59 0.35185597289788717 1107 60-61 0.2874381066844123 1107 62-63 0.27986325987755123 1107 64-65 0.2825663877396707 1107 66-67 0.26469017667375283 1107 68-69 0.40064392463920484 1107 70-71 0.4654973071655739 1107 72-73 0.3037802452295004 1107 74-75 0.2892346735468543 1107 76-77 0.2697488856622243 1107 78-79 0.27916653952468096 1107 80-81 0.30025397838488743 1107 82-83 0.3814135840363626 1107 84-85 0.3079719088983346 1107 86-87 0.26833582334912975 1107 88-89 0.37684848996056175 1107 90-91 0.5216148858274892 1107 92-93 0.6694437321387205 1107 94-95 0.6018915620409899 1107 96-97 0.4320287383152568 1107 98-99 0.6820909294821753 1107 100-101 0.9840696022012629 1108 1 0.0778289320421095 1108 2 0.09727193875066575 1108 3 0.019556522581865465 1108 4 0.09141925609895907 1108 5 0.1837369784713303 1108 6 0.1490378125486913 1108 7 0.14185772272460184 1108 8 0.10997043024693198 1108 9 0.13159170856149416 1108 10-11 0.19621680572700484 1108 12-13 0.20474455602319352 1108 14-15 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0.09615724056365238 2205 100-101 -0.06803501762816566 2206 1 0.30739513855969136 2206 2 0.31140693401165365 2206 3 0.2925369640664641 2206 4 0.30041318652752835 2206 5 0.3403981673877041 2206 6 0.29036730860027404 2206 7 0.298676869443959 2206 8 0.37745458152837585 2206 9 0.29508063318731814 2206 10-11 0.3275242468443196 2206 12-13 0.3567787755327885 2206 14-15 0.4504793799540536 2206 16-17 0.41982531608366713 2206 18-19 0.41515742398116373 2206 20-21 0.44952352087800307 2206 22-23 0.37872360080272216 2206 24-25 0.44849613570522706 2206 26-27 0.4415180085808714 2206 28-29 0.43644191079901873 2206 30-31 0.5461570971347314 2206 32-33 0.4962182640736472 2206 34-35 0.5265255103567767 2206 36-37 0.5511629342668058 2206 38-39 0.5417002753493634 2206 40-41 0.5962261777827109 2206 42-43 0.6006136430457545 2206 44-45 0.7582279675344878 2206 46-47 0.7305535579300013 2206 48-49 0.7612684243204129 2206 50-51 0.7388179282418648 2206 52-53 0.7126415313379937 2206 54-55 0.7489608983591083 2206 56-57 0.6989587605232472 2206 58-59 0.7043161878387423 2206 60-61 0.7334340145463756 2206 62-63 0.6994816769855063 2206 64-65 0.7230319133417851 2206 66-67 0.5795742304600822 2206 68-69 0.6379683958180848 2206 70-71 0.632550428975069 2206 72-73 0.5245969320897537 2206 74-75 0.5399052619609357 2206 76-77 0.6466228348241074 2206 78-79 0.6158629600782106 2206 80-81 0.559675000258899 2206 82-83 0.6867543077175711 2206 84-85 0.5925892553084573 2206 86-87 0.4859776908119393 2206 88-89 0.34794141686160174 2206 90-91 0.39960249888972754 2206 92-93 0.4220088616517117 2206 94-95 0.4625583788356096 2206 96-97 0.4837505447690127 2206 98-99 0.48993592559312305 2206 100-101 0.16078812748423843 2207 1 0.25209436352387016 2207 2 0.2381882412251244 2207 3 0.33850134678618105 2207 4 0.21213358198099996 2207 5 0.19761414569254043 2207 6 0.22552279398873054 2207 7 0.07705352468586568 2207 8 0.2545577377060724 2207 9 0.284467072277792 2207 10-11 0.31746795863723776 2207 12-13 0.3572673725381392 2207 14-15 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2316 48-49 -1.9400263911536797 2316 50-51 -2.2822233513128296 2316 52-53 -2.0755946822961384 2316 54-55 -1.9259472631104302 2316 56-57 -1.819357932256274 2316 58-59 -1.595976018162375 2316 60-61 -1.5685207980995273 2316 62-63 -1.8905995043516874 2316 64-65 -1.9685826587213526 2316 66-67 -2.0052567559533294 2316 68-69 -2.108510738693113 2316 70-71 -1.9730899340202193 2316 72-73 -2.06670917575525 2316 74-75 -2.2316278189459346 2316 76-77 -2.2182179890731746 2316 78-79 -2.0187196757575414 2316 80-81 -1.89530034921507 2316 82-83 -1.885831638423248 2316 84-85 -1.890471119978585 2316 86-87 -2.0869059827137413 2316 88-89 -2.08029421598318 2316 90-91 -2.357000095121421 2316 92-93 -2.5120000893028624 2316 94-95 -2.453377048460247 2316 96-97 -2.3604336025564656 2316 98-99 -2.635850543801997 2316 100-101 -2.945339666374778 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13904.0 3 4502.0 4 4662.0 5 5168.0 6 6016.0 7 7408.0 8 8887.0 9 11051.0 10 13314.0 11 17054.0 12 21477.0 13 26990.0 14 28855.0 15 28323.0 16 35211.0 17 43225.0 18 36729.0 19 27606.0 20 27774.0 21 29400.0 22 32352.0 23 36101.0 24 41210.0 25 47035.0 26 54601.0 27 63410.0 28 74319.0 29 87655.0 30 104516.0 31 127043.0 32 155601.0 33 196387.0 34 260631.0 35 366905.0 36 536726.0 37 791585.0 38 709574.0 39 114497.0 40 64.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6540999057493 18.716567842613248 14.13421777878524 22.49511447285222 2 15.26545618079124 20.614329490386034 40.208138648457954 23.912075680364772 3 19.65081099201943 25.29638934674531 29.658986838770346 25.393812822464913 4 12.548546687569425 16.404762165944177 37.50370225874747 33.54298888773893 5 13.465358012011453 37.902406178272194 33.81787330181832 14.814362507898036 6 31.29286086056315 36.057124935893405 17.766869329306772 14.88314487423668 7 28.393864228271603 33.335136644944306 20.617118654408596 17.65388047237549 8 26.47704279228285 34.03813291704899 19.888437384558554 19.596386906109608 9 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26.560641295671616 29.418638102349803 22.214048375560196 34-35 22.533364920292506 26.41636271386456 29.158359260564097 21.89191310527883 36-37 22.405978648777122 26.143615480761547 29.30903168886823 22.141374181593104 38-39 22.199553995222164 26.535621155817147 29.436363196924344 21.82846165203635 40-41 22.2358323086892 26.47201558216673 29.40693769107036 21.885214418073705 42-43 21.89208463509104 26.664540778170913 28.91681394327587 22.526560643462176 44-45 22.191716201222146 26.604950711162722 28.76639331314615 22.436939774468986 46-47 22.415551375207023 26.85949720937802 28.340656921029705 22.384294494385248 48-49 22.492087229875175 26.885932234731452 28.17655905059924 22.44542148479414 50-51 22.179694123199926 27.168059430993903 28.246847431631522 22.40539901417465 52-53 22.531711500683503 27.21281562795988 27.92953037082463 22.325942500531987 54-55 22.014434498041044 26.905778454425207 28.229078396707795 22.85070865082595 56-57 22.3608228168492 27.301270185733088 27.84235210309941 22.495554894318293 58-59 22.63102938901936 26.79603383947244 27.899466292527965 22.67347047898024 60-61 22.423309090500332 26.740082374131436 28.53349316111985 22.303115374248385 62-63 22.291140842943637 26.77100939728307 28.4694075588434 22.468442200929893 64-65 22.12123979680572 27.05275527092471 28.193427893836148 22.632577038433425 66-67 22.14148999967681 27.1935773853485 27.78289561096594 22.882037004008748 68-69 22.06326628141502 26.969394713101174 28.14609937500359 22.82123963048021 70-71 22.231607425563308 26.978664823144584 27.93896026551506 22.850767485777048 72-73 22.288456251048103 26.733224943122668 28.085437308775525 22.8928814970537 74-75 22.065131839743234 27.357081849988607 27.811313284321514 22.76647302594664 76-77 22.299289023160448 26.909252176531144 27.863631451854843 22.927827348453565 78-79 22.197361831619787 27.002236478065072 27.96315398200529 22.837247708309853 80-81 22.2959487297412 27.200080208484636 27.720932859133367 22.7830382026408 82-83 22.287388660816326 27.046655274538832 27.78125247913993 22.884703585504916 84-85 22.09032380960586 27.049460827846605 27.726605820297646 23.13360954224989 86-87 22.262390611941285 26.962140076070234 27.877010054941742 22.898459257046742 88-89 22.28139907369431 26.903185508757826 27.54554842962594 23.26986698792193 90-91 22.187670215873972 26.888994505097962 27.888264540437635 23.035070738590427 92-93 22.565477881081225 26.949788252467354 27.68983639371744 22.794897472733982 94-95 22.37769544956836 26.70101964944972 27.705862948427672 23.215421952554248 96-97 22.33089637403006 26.477876631583534 27.852327424009253 23.33889957037715 98-99 22.3092829554019 26.661254395076917 27.445160056382417 23.584302593138766 100-101 22.57629029324194 26.161192115113973 27.918441787215304 23.344075804428783 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4732.0 1 2864.5 2 1322.5 3 1692.0 4 1456.5 5 1400.0 6 2389.5 7 4843.5 8 7704.5 9 8607.0 10 7266.5 11 6037.0 12 6075.5 13 6407.5 14 6871.0 15 7237.5 16 7514.5 17 7819.5 18 7989.0 19 8362.0 20 8972.0 21 9922.0 22 11212.0 23 12968.0 24 14857.5 25 17444.5 26 22341.0 27 28143.5 28 32962.0 29 38080.5 30 44287.5 31 52252.0 32 61702.5 33 69094.5 34 77115.0 35 84903.5 36 92942.5 37 103190.0 38 109728.5 39 112067.0 40 113976.5 41 119795.5 42 126622.0 43 133431.5 44 139727.0 45 143581.0 46 147092.0 47 157577.0 48 189075.0 49 194361.5 50 169836.0 51 157995.0 52 148759.5 53 145636.5 54 142922.5 55 131810.0 56 118559.5 57 104965.5 58 94250.0 59 84737.5 60 72912.5 61 60170.0 62 47898.0 63 36545.5 64 26873.5 65 20147.5 66 14833.0 67 11386.5 68 8372.5 69 5682.5 70 4089.5 71 3089.5 72 2459.0 73 2034.5 74 1699.0 75 1342.5 76 985.5 77 612.5 78 375.0 79 260.0 80 179.5 81 138.5 82 120.5 83 101.0 84 75.5 85 54.5 86 42.5 87 35.5 88 30.5 89 22.0 90 12.5 91 13.0 92 14.0 93 8.5 94 7.0 95 8.0 96 5.5 97 3.0 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16241964777472218 2 0.09588428898405058 3 0.08830883459972061 4 0.10438880852872288 5 0.1255190853806118 6 0.038877803632787714 7 0.042498775539763035 8 0.04569094814196497 9 0.04671530203670141 10-11 0.044678505339027785 12-13 0.049014142753958774 14-15 0.04725130116766815 16-17 0.04528597102079009 18-19 0.04982409699630852 20-21 0.052075293346368824 22-23 0.06492736139777139 24-25 0.0809954242349744 26-27 0.08286546564745836 28-29 0.07281250416888213 30-31 0.061461233684186455 32-33 0.05090800635004126 34-35 0.2976939173389287 36-37 0.472489189493083 38-39 0.4499772259924798 40-41 0.4254999323449986 42-43 0.45555161695453394 44-45 0.46548546751511755 46-47 0.4642467139679944 48-49 0.4808626870279635 50-51 0.47039283733641307 52-53 0.47703922656040065 54-55 0.4681654631699512 56-57 0.44627287644290964 58-59 0.42538082142700595 60-61 0.3954720699190618 62-63 0.4527882436571054 64-65 0.4903439161001752 66-67 0.4919995578602724 68-69 0.5027433626632057 70-71 0.4998013229887883 72-73 0.4901771608149855 74-75 0.48292330590923555 76-77 0.46459213563017304 78-79 0.43901902153715977 80-81 0.4444981237648198 82-83 0.4562305491870918 84-85 0.45374113100104624 86-87 0.46510431257754126 88-89 0.47619353904265316 90-91 0.4804577099067886 92-93 0.4828637504502392 94-95 0.4871636545897725 96-97 0.48834285267789934 98-99 0.48179175218830583 100-101 0.46805826334375794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4197768.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.31484764571988 #Duplication Level Percentage of deduplicated Percentage of total 1 66.87582456116475 25.623370292429186 2 14.614070444995257 11.198717651676219 3 6.389010826904393 7.343819293190898 4 3.4752794250239054 5.326192067843837 5 2.091709824511179 4.0071771622600645 6 1.4118981912982316 3.245799845451552 7 1.0035458117787706 2.6915493418662715 8 0.7108195010312389 2.1787952708494833 9 0.5163504442449243 1.7805499742739643 >10 2.605821464925316 17.456223257570596 >50 0.17962745078013684 4.7992553664231785 >100 0.1143193126613266 8.292841829817128 >500 0.007544955508590452 1.930745435198727 >1k 0.0038660101591266776 2.6185259583701317 >5k 6.235500256655924E-5 0.14377738743336288 >10k+ 2.4942001026623695E-4 1.3626598653455109 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20415 0.4863298781638242 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15179 0.36159692484196365 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10813 0.2575892712508171 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10629 0.25320598946868905 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6018 0.14336190089590467 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4764 0.11348888266335824 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4358 0.10381707612235837 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4285 0.10207805671966627 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.382218359852188E-5 0.0 5 2.382218359852188E-5 0.0 0.0 2.382218359852188E-5 0.0 6 4.764436719704376E-5 0.0 0.0 2.382218359852188E-5 0.0 7 1.6675528518965315E-4 0.0 0.0 2.382218359852188E-5 0.0 8 1.6675528518965315E-4 0.0 0.0 2.382218359852188E-5 0.0 9 1.6675528518965315E-4 0.0 0.0 2.382218359852188E-5 0.0 10-11 2.1439965238669693E-4 0.0 0.0 2.382218359852188E-5 0.0 12-13 2.5013292778447975E-4 0.0 0.0 4.764436719704376E-5 0.0 14-15 3.573327539778282E-4 0.0 0.0 9.528873439408752E-5 0.0 16-17 3.6924384577708915E-4 0.0 0.0 1.786663769889141E-4 0.0 18-19 4.407103965726548E-4 0.0 0.0 1.9057746878817504E-4 0.0 20-21 5.002658555689595E-4 0.0 0.0 3.0968838678078445E-4 0.0 22-23 5.479102227660033E-4 0.0 0.0 7.384876915541783E-4 0.0 24-25 6.670211407586126E-4 0.0 0.0 0.001381686648714269 0.0 26-27 6.670211407586126E-4 0.0 0.0 0.00240604054345071 0.0 28-29 7.384876915541783E-4 0.0 0.0 0.0056696796964482085 0.0 30-31 8.337764259482659E-4 0.0 0.0 0.016377751223983793 0.0 32-33 8.337764259482659E-4 0.0 0.0 0.036436029813939216 0.0 34-35 8.695097013460486E-4 0.0 0.0 0.06767882360340066 0.0 36-37 0.001000531711137919 0.0 0.0 0.11818185283226706 0.0 38-39 0.001024353894736441 0.0 0.0 0.19186386670249522 0.0 40-41 0.001024353894736441 0.0 0.0 0.2733238235176408 0.0 42-43 0.0010719982619334847 0.0 0.0 0.35909559556411885 0.0 44-45 0.0011315537209297894 0.0 0.0 0.46041134240863235 0.0 46-47 0.0012863979143201815 0.0 0.0 0.5883841126998919 0.0 48-49 0.0013578644651157472 0.0 0.0 0.7253378462077942 0.0 50-51 0.0013578644651157472 0.0 0.0 0.8713797427585326 0.0 52-53 0.0013578644651157472 0.0 0.0 1.0136458232089054 0.0 54-55 0.0013816866487142692 0.0 0.0 1.1721586328734699 0.0 56-57 0.001405508832312791 0.0 0.0 1.364332187962746 0.0 58-59 0.001405508832312791 0.0 0.0 1.5823647233482174 0.0 60-61 0.001405508832312791 0.0 0.0 1.821765757421563 0.0 62-63 0.001405508832312791 0.0 0.0 2.057331419935547 0.0 64-65 0.001429331015911313 0.0 0.0 2.2978282744544245 0.0 66-67 0.0014769753831083566 0.0 0.0 2.576309600721145 0.0 68-69 0.0014769753831083566 0.0 0.0 2.899076366297518 0.0 70-71 0.0015127086585061396 0.0 0.0 3.2533241474993377 0.0 72-73 0.0015484419339039223 0.0 0.0 3.603950956794182 0.0 74-75 0.001560353025703183 0.0 0.0 3.965929036573722 0.0 76-77 0.001572264117502444 0.0 0.0 4.351288589555211 0.0 78-79 0.001572264117502444 0.0 0.0 4.789569123400817 0.0 80-81 0.0018343081370861848 0.0 0.0 5.274064693427555 0.0 82-83 0.0018581303206847068 0.0 0.0 5.766945672080973 0.0 84-85 0.0018938635960824895 0.0 0.0 6.262542379664621 0.0 86-87 0.0019295968714802725 0.0 0.0 6.775576925642389 0.0 88-89 0.0019534190550787945 0.0 0.0 7.32417799173275 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 23700 0.0 26.544008 1 TACATGG 24000 0.0 25.666014 2 ACATGGG 24340 0.0 24.29519 3 GTATCAA 37730 0.0 22.503635 1 GAGTACT 17065 0.0 22.101858 12-13 CATGGGG 9435 0.0 21.846087 4 AGTACTT 17895 0.0 20.65335 12-13 GTACTTT 18280 0.0 20.581146 14-15 CATGGGA 14750 0.0 20.431084 4 TATCAAC 43565 0.0 19.392775 2 ATCAACG 43525 0.0 19.36705 3 CAACGCA 43475 0.0 19.356627 5 TCAACGC 43565 0.0 19.349268 4 GGTATCA 28630 0.0 19.058903 1 AACGCAG 44290 0.0 19.018017 6 AGAGTAC 33970 0.0 17.828783 10-11 TACCTGG 2915 0.0 17.71798 2 ACTTTTT 21725 0.0 17.68913 16-17 ACGCAGA 47835 0.0 17.501612 7 TACTTTT 21020 0.0 17.45906 14-15 >>END_MODULE