FastQCFastQC Report
Thu 26 May 2016
SRR938735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4197768
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197830.47127425812955837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161400.38449004328014313No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118280.2817687876033168No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110200.2625204632557111No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57260.1364058232851363No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43990.10479378564989775No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43590.10384089830595689No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG234850.024.3384281
TACATGG238850.023.5810782
ACATGGG239000.022.4743273
GAGTACT178200.021.31658612-13
GTATCAA395850.021.2621781
AGTACTT188450.020.30823912-13
ATACCGT2851.9936124E-919.9790366
CATGGGA148450.019.8175354
GTACTTT190850.019.74205414-15
TACCTGG28850.019.4077132
CATGGGG86200.018.9360474
GGTATCA305700.018.5574461
ATCAACG458000.018.2134233
TCAACGC459150.018.1781394
TATCAAC461100.018.1321332
CAACGCA461850.018.0615945
AGAGTAC358250.017.76922410-11
AACGCAG470450.017.7515956
TACTTTT218000.017.37042414-15
ACTTTTT219950.017.17245716-17
Produced by FastQC (version 0.11.5)