##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938735_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4197768 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.009696343390104 33.0 31.0 34.0 30.0 34.0 2 32.22882017300623 34.0 31.0 34.0 30.0 34.0 3 32.31533805584301 34.0 31.0 34.0 30.0 34.0 4 35.94555082605804 37.0 35.0 37.0 35.0 37.0 5 35.85913037595217 37.0 35.0 37.0 35.0 37.0 6 35.89172769910105 37.0 35.0 37.0 35.0 37.0 7 35.82691944862127 37.0 35.0 37.0 33.0 37.0 8 35.79530336121482 37.0 35.0 37.0 33.0 37.0 9 37.44379727512335 39.0 37.0 39.0 34.0 39.0 10-11 37.371855829097754 39.0 37.0 39.0 34.0 39.0 12-13 37.29925319836637 39.0 37.0 39.0 34.0 39.0 14-15 38.510802526485506 40.0 38.0 41.0 34.0 41.0 16-17 38.45161559666947 40.0 38.0 41.0 33.5 41.0 18-19 38.486003871581275 40.0 38.0 41.0 33.5 41.0 20-21 38.56637217683302 40.0 38.0 41.0 34.0 41.0 22-23 38.52277162530183 40.0 38.0 41.0 34.0 41.0 24-25 38.450050002763376 40.0 38.0 41.0 34.0 41.0 26-27 38.28512378483042 40.0 38.0 41.0 33.5 41.0 28-29 38.12928942237875 40.0 38.0 41.0 33.0 41.0 30-31 37.90211631514653 40.0 37.0 41.0 33.0 41.0 32-33 37.678364073479045 40.0 37.0 41.0 33.0 41.0 34-35 37.635486525220074 40.0 37.0 41.0 33.0 41.0 36-37 37.54450889139181 40.0 37.0 41.0 32.0 41.0 38-39 37.44531522466225 40.0 36.5 41.0 32.0 41.0 40-41 37.28746359970346 40.0 36.0 41.0 31.5 41.0 42-43 37.100999745579074 40.0 36.0 41.0 31.0 41.0 44-45 36.8379432831924 40.0 36.0 41.0 30.5 41.0 46-47 36.515493114436055 39.5 35.5 41.0 30.0 41.0 48-49 36.22272490999979 39.0 35.0 41.0 29.5 41.0 50-51 35.97972434398471 39.0 35.0 41.0 29.0 41.0 52-53 35.77148236872547 39.0 35.0 41.0 28.0 41.0 54-55 35.530804703833084 39.0 35.0 40.0 28.0 41.0 56-57 35.280467858156996 38.0 34.5 40.0 27.5 41.0 58-59 34.98280693454235 38.0 34.0 40.0 26.5 41.0 60-61 34.6313525425893 38.0 34.0 40.0 26.0 41.0 62-63 34.26629544557965 37.0 34.0 40.0 26.0 41.0 64-65 33.8999758919502 36.5 33.0 39.5 25.5 41.0 66-67 33.50444807812151 36.0 33.0 39.0 24.5 40.5 68-69 33.063741254876405 35.5 32.5 39.0 23.5 40.0 70-71 32.58969742968168 35.0 32.0 38.0 22.5 40.0 72-73 32.235620096203505 35.0 32.0 37.0 22.0 39.5 74-75 31.816062250224405 35.0 31.0 37.0 20.5 39.0 76-77 29.775585382517566 33.0 28.5 35.0 18.0 37.5 78-79 30.84296440394038 34.0 30.5 36.0 18.5 38.0 80-81 30.89293691314051 34.0 31.0 35.5 18.5 37.0 82-83 30.595412490637884 34.0 31.0 35.0 17.5 37.0 84-85 30.225225048168454 34.0 30.5 35.0 13.5 36.5 86-87 29.71557265670709 34.0 30.0 35.0 7.0 36.0 88-89 29.212506860788878 34.0 29.0 35.0 2.0 35.5 90-91 28.804848433739075 34.0 29.0 35.0 2.0 35.0 92-93 28.540119177620106 34.0 29.0 35.0 2.0 35.0 94-95 28.170552064811588 34.0 29.0 35.0 2.0 35.0 96-97 27.664958020548063 33.5 28.0 35.0 2.0 35.0 98-99 26.957504797787777 33.0 26.5 35.0 2.0 35.0 100-101 23.79624112147217 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04952696079818253 1101 2 0.10056550673341746 1101 3 0.12923867304961334 1101 4 0.11946992156974545 1101 5 0.11296873313671796 1101 6 0.12136535694157402 1101 7 0.14501445459057294 1101 8 0.09215182186419213 1101 9 0.1875037805194424 1101 10-11 0.171365525703564 1101 12-13 0.15858987117619705 1101 14-15 0.2657126842930708 1101 16-17 0.2204830531894615 1101 18-19 0.19077965125912044 1101 20-21 0.25804516478074646 1101 22-23 0.20432339065244776 1101 24-25 0.19637286393498243 1101 26-27 0.2127880724152149 1101 28-29 0.24955447040039758 1101 30-31 0.15762682369969383 1101 32-33 0.22055744046475212 1101 34-35 0.22190950041099455 1101 36-37 0.25021768582991655 1101 38-39 0.22529563633094085 1101 40-41 0.35766163388298366 1101 42-43 0.28923562308821715 1101 44-45 0.2502260742804836 1101 46-47 0.26981884151542346 1101 48-49 0.1490268183018415 1101 50-51 0.18351645680701267 1101 52-53 0.2007177267935063 1101 54-55 0.23601375121082668 1101 56-57 0.07239459175335128 1101 58-59 0.18321592325721525 1101 60-61 0.25818851286032896 1101 62-63 0.1999039923319117 1101 64-65 0.10627733984509291 1101 66-67 -0.007228817869851412 1101 68-69 0.06453697349942189 1101 70-71 -0.01321154754607079 1101 72-73 0.07905523448655316 1101 74-75 0.17323174340126357 1101 76-77 0.044488681529408325 1101 78-79 0.22268127835593532 1101 80-81 0.29301062265000866 1101 82-83 0.25920889121851687 1101 84-85 0.28769313518850836 1101 86-87 0.42728870690450194 1101 88-89 0.4933715829875922 1101 90-91 0.6442292402045453 1101 92-93 0.5801107909342171 1101 94-95 0.6549098349774525 1101 96-97 0.4460468936301609 1101 98-99 0.4212639546426793 1101 100-101 0.9218891218376726 1102 1 0.07989060290296379 1102 2 0.11035642752506192 1102 3 0.11527364073170077 1102 4 0.06372112709919975 1102 5 0.10448332296483898 1102 6 0.13871056414193106 1102 7 0.09998349030664855 1102 8 0.11471366205611133 1102 9 0.12288537646167441 1102 10-11 0.20749980151219916 1102 12-13 0.24821922944617825 1102 14-15 0.19617309332328858 1102 16-17 0.236090210922697 1102 18-19 0.23096623077348966 1102 20-21 0.21872806603231965 1102 22-23 0.17483967088251973 1102 24-25 0.10788289469735446 1102 26-27 0.14810705039445082 1102 28-29 0.1739360936416645 1102 30-31 0.26860283740305846 1102 32-33 0.26989026824164597 1102 34-35 0.20282567763436532 1102 36-37 0.2142942856827048 1102 38-39 0.2135925246050192 1102 40-41 0.25850934460673614 1102 42-43 0.22609416637362045 1102 44-45 0.19851269459245913 1102 46-47 0.21482231176457134 1102 48-49 0.25567882735405334 1102 50-51 0.1972369928696054 1102 52-53 0.2340746710721433 1102 54-55 0.20219596262693074 1102 56-57 0.19662322084957395 1102 58-59 0.10955603258170044 1102 60-61 0.015119575986908274 1102 62-63 -0.017940504275642866 1102 64-65 0.14938111130204135 1102 66-67 0.054963431039041666 1102 68-69 0.0763650066462489 1102 70-71 0.0959952177854575 1102 72-73 0.1543728814802492 1102 74-75 0.1530376233129047 1102 76-77 -0.037125231521507374 1102 78-79 -1.3233573902482476E-4 1102 80-81 -0.02824490807921265 1102 82-83 0.18617948889266245 1102 84-85 0.172860251241012 1102 86-87 0.2418484872138933 1102 88-89 0.4353896765911003 1102 90-91 0.3777322846766964 1102 92-93 0.5203314250594993 1102 94-95 0.42938204197413654 1102 96-97 0.4999352018028773 1102 98-99 0.6198060159870238 1102 100-101 1.0001587764871829 1103 1 0.011720536746544497 1103 2 0.08847915194561295 1103 3 0.018750822963973235 1103 4 0.08858994236475581 1103 5 0.10563224850400843 1103 6 0.09612551557041371 1103 7 0.08745630439471341 1103 8 0.12658153513493886 1103 9 0.1281488643536548 1103 10-11 0.038172845288535484 1103 12-13 0.12244274847970615 1103 14-15 0.14481314410260637 1103 16-17 0.05603777092052553 1103 18-19 0.10110381375951505 1103 20-21 0.1275498944555622 1103 22-23 0.05558587211733368 1103 24-25 0.10731502868734566 1103 26-27 0.03634632321608677 1103 28-29 0.13759787378389632 1103 30-31 0.04494666390126412 1103 32-33 0.19789656288173063 1103 34-35 0.18069687790084998 1103 36-37 0.09689127136301323 1103 38-39 0.2600814628680652 1103 40-41 0.1704544663453973 1103 42-43 0.16886915320430518 1103 44-45 0.27015943460701664 1103 46-47 0.26263459631076813 1103 48-49 0.32254334032194976 1103 50-51 0.4572178901063779 1103 52-53 0.44382560163888485 1103 54-55 0.382815118745242 1103 56-57 0.341086929714848 1103 58-59 0.17379860596187058 1103 60-61 0.2677343741284872 1103 62-63 0.3615313119466208 1103 64-65 0.31663729022649534 1103 66-67 0.21391519746968868 1103 68-69 0.2646974087672831 1103 70-71 0.10114395837327095 1103 72-73 0.3493723682644614 1103 74-75 0.2871383675726342 1103 76-77 0.2212340732744451 1103 78-79 0.15898900977721198 1103 80-81 0.3627352275646878 1103 82-83 0.4181441902979053 1103 84-85 0.30879671028445443 1103 86-87 0.4516759765041449 1103 88-89 0.5622196419054575 1103 90-91 0.5707230884307251 1103 92-93 0.4830038728276911 1103 94-95 0.3815215224060893 1103 96-97 0.5978557458309837 1103 98-99 0.6746081422497276 1103 100-101 0.9982806366717085 1104 1 -0.03989728741276011 1104 2 -0.013095093651465106 1104 3 -0.033388601588065114 1104 4 -0.002241995879295189 1104 5 -0.0017757235443482955 1104 6 0.053642649759787275 1104 7 0.0026294824368733316 1104 8 -0.028886064275958745 1104 9 0.015985483577694026 1104 10-11 -0.006027874021420132 1104 12-13 -0.06626213404514658 1104 14-15 0.02268973670968677 1104 16-17 0.10897098209964895 1104 18-19 0.09920360387288696 1104 20-21 0.02880759621135809 1104 22-23 -0.0010774051056046119 1104 24-25 -0.03095204269903462 1104 26-27 -0.02463226240308103 1104 28-29 0.016075649196572783 1104 30-31 0.003678029151934936 1104 32-33 0.04616448282757801 1104 34-35 0.04750324526093408 1104 36-37 0.017628926021366453 1104 38-39 0.15390935535318562 1104 40-41 0.25735762221953706 1104 42-43 0.23134086788888908 1104 44-45 0.12750616175631535 1104 46-47 0.18342163041806003 1104 48-49 0.1536711381711129 1104 50-51 0.2567262409861968 1104 52-53 0.16856717453254078 1104 54-55 0.1790145072071283 1104 56-57 0.13055093737010282 1104 58-59 0.1515473513403336 1104 60-61 0.2629991606366744 1104 62-63 0.10325156490362986 1104 64-65 0.31620713769673614 1104 66-67 0.23434906064930772 1104 68-69 0.19843486255195586 1104 70-71 0.0974662681041849 1104 72-73 0.07910689859398445 1104 74-75 0.06361162500543927 1104 76-77 0.031035522345536748 1104 78-79 0.06241790912367762 1104 80-81 0.1213591325873935 1104 82-83 -0.09985905340453627 1104 84-85 0.0714295793550832 1104 86-87 0.15993697853301825 1104 88-89 0.33534066997599865 1104 90-91 0.33681036157290833 1104 92-93 0.42886727728660645 1104 94-95 0.5303509418188312 1104 96-97 0.5173243965926737 1104 98-99 0.5766835413123808 1104 100-101 1.0832121502524927 1105 1 0.03740808467599521 1105 2 0.024641081585180302 1105 3 0.03696904574004378 1105 4 -0.010819267278648681 1105 5 0.013717227415050104 1105 6 -0.007941178256274384 1105 7 0.044631163002840424 1105 8 -0.00674668572395376 1105 9 -5.742352437394516E-4 1105 10-11 0.034000526941142084 1105 12-13 0.006681928406855775 1105 14-15 0.027828805049686878 1105 16-17 -0.0062639809007976055 1105 18-19 -0.07284566261126457 1105 20-21 -0.09011657050800892 1105 22-23 -0.04562740194324988 1105 24-25 -0.007501491395096593 1105 26-27 -0.037585209567659206 1105 28-29 -0.08359802585183473 1105 30-31 -0.1156913369614685 1105 32-33 0.005857674125984147 1105 34-35 0.08082796494073108 1105 36-37 0.019694275341159084 1105 38-39 0.005291184158330964 1105 40-41 -0.02744388727806779 1105 42-43 -0.019053824172992506 1105 44-45 0.026650699465143646 1105 46-47 0.1282733460711185 1105 48-49 0.06559752456274737 1105 50-51 0.08488255771565889 1105 52-53 0.07716682336050695 1105 54-55 0.15674755020613418 1105 56-57 0.14467648588816218 1105 58-59 0.055927062873301736 1105 60-61 0.12449269587887102 1105 62-63 0.1183705146402545 1105 64-65 0.042406378129101086 1105 66-67 0.12348796635389192 1105 68-69 0.11235467726805126 1105 70-71 0.10784623262557602 1105 72-73 -0.22048588039351813 1105 74-75 -0.06569048835144642 1105 76-77 -0.11223772623667472 1105 78-79 -0.2925340682519284 1105 80-81 -0.05239238459022033 1105 82-83 -0.0012172097212648225 1105 84-85 0.04889478591712404 1105 86-87 0.07186071456971277 1105 88-89 0.25924804609449126 1105 90-91 0.47173625704652267 1105 92-93 0.31463128727362744 1105 94-95 0.30255835618676485 1105 96-97 0.27383143960974365 1105 98-99 0.505251295528101 1105 100-101 0.9878872257725533 1106 1 -0.05863132929886561 1106 2 -0.11093476826747661 1106 3 -0.12587860500470072 1106 4 -0.07215573116456397 1106 5 8.678187140631621E-4 1106 6 -0.056357438457631304 1106 7 -0.05494668913217993 1106 8 -0.03821326816763815 1106 9 -0.039176630235651544 1106 10-11 -0.12985198946893206 1106 12-13 -0.08620618512732392 1106 14-15 -0.03876365004048665 1106 16-17 -0.133532385063873 1106 18-19 -0.2150104516416178 1106 20-21 -0.14387406789342805 1106 22-23 -0.19481573268930674 1106 24-25 -0.13702687300568073 1106 26-27 -0.1603688885827239 1106 28-29 -0.17527693365448016 1106 30-31 -0.17236066539238237 1106 32-33 -0.08994790187285417 1106 34-35 -0.1099464296817132 1106 36-37 -0.23387249868995497 1106 38-39 -0.2209663355739906 1106 40-41 -0.09194073934455815 1106 42-43 -0.0030613386910331997 1106 44-45 0.07143904451321959 1106 46-47 0.045469376069597445 1106 48-49 -0.09635288319370972 1106 50-51 -0.025709992721303365 1106 52-53 0.054656789601786215 1106 54-55 -0.028844985332888484 1106 56-57 -0.1425887301169837 1106 58-59 -0.07907861323965193 1106 60-61 -0.038047252931825426 1106 62-63 -0.15237551431536644 1106 64-65 -0.06843982453643349 1106 66-67 -0.11725225240737558 1106 68-69 -0.07346344279142158 1106 70-71 -0.090336636537792 1106 72-73 -0.08091866538354964 1106 74-75 0.2532986272177844 1106 76-77 0.09262040073544142 1106 78-79 0.07070116715032881 1106 80-81 -0.09176544972100942 1106 82-83 0.09525570434447062 1106 84-85 -0.018052806129766452 1106 86-87 0.16683981276795734 1106 88-89 0.3583111746010914 1106 90-91 0.3333409731365897 1106 92-93 0.4835022920201091 1106 94-95 0.43814341296279125 1106 96-97 0.36742464257621776 1106 98-99 0.3445801733858964 1106 100-101 0.8484479542219461 1107 1 -0.09529892516173888 1107 2 -0.04622834158249134 1107 3 -0.12171538450644448 1107 4 -0.07698981216557144 1107 5 -0.04125500186581377 1107 6 -0.04885888254349169 1107 7 -0.06939795087525624 1107 8 -7.409500721635709E-4 1107 9 -0.09492788536897478 1107 10-11 -0.06177324152729824 1107 12-13 -0.07009521610979164 1107 14-15 -0.14862452925168412 1107 16-17 -0.05953874766305489 1107 18-19 -0.06321412378671454 1107 20-21 -0.10409486215501573 1107 22-23 -0.17758079069701438 1107 24-25 -0.14238151462811288 1107 26-27 -0.1561759817025532 1107 28-29 -0.1231442559313436 1107 30-31 -0.10779065812166522 1107 32-33 -0.020767366713315027 1107 34-35 -0.0675184974689742 1107 36-37 0.009193676364866121 1107 38-39 0.03261321559551078 1107 40-41 0.0018573070443679285 1107 42-43 -0.06963283459155178 1107 44-45 -0.14749675760821646 1107 46-47 -0.003706905861939447 1107 48-49 -0.2481978070523141 1107 50-51 -0.11243693260996679 1107 52-53 -0.03898811184114237 1107 54-55 -0.10321397010461197 1107 56-57 -0.20772050833988942 1107 58-59 -0.16621690924707622 1107 60-61 -0.24173230139850688 1107 62-63 -0.2373241163540456 1107 64-65 -0.18023129566477536 1107 66-67 -0.1696989505517621 1107 68-69 -0.13701839420978956 1107 70-71 -0.19920789305054853 1107 72-73 -0.1144852269648311 1107 74-75 -0.149482552823077 1107 76-77 -0.1256773022340596 1107 78-79 -0.23955261836627884 1107 80-81 -0.16128046677702912 1107 82-83 -0.10393184289366175 1107 84-85 -0.11158149099143344 1107 86-87 -0.030365299152290248 1107 88-89 0.07766166645242478 1107 90-91 0.2437397419995122 1107 92-93 0.30336182054403693 1107 94-95 0.27260591127443234 1107 96-97 0.19145368522632467 1107 98-99 0.20069617155816033 1107 100-101 0.7033756637149793 1108 1 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-2.566498395681844 2316 100-101 -3.4180597644176203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 49.0 5 107.0 6 310.0 7 695.0 8 1591.0 9 2885.0 10 4681.0 11 7261.0 12 10202.0 13 14180.0 14 16953.0 15 22641.0 16 29256.0 17 39430.0 18 37041.0 19 25180.0 20 22648.0 21 23298.0 22 24810.0 23 27799.0 24 32336.0 25 38884.0 26 47192.0 27 58115.0 28 71398.0 29 87084.0 30 105729.0 31 128455.0 32 158247.0 33 198762.0 34 264166.0 35 372561.0 36 562543.0 37 896903.0 38 783825.0 39 80520.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.229859712729166 19.62699274409172 14.71748831736587 22.42565922581324 2 15.719663402074627 21.297151247996553 39.22334440588427 23.759840944044548 3 19.681221067958017 25.342467711412354 30.016856577114314 24.959454643515315 4 12.974657008200548 17.134081731053264 36.99430268657058 32.89695857417561 5 13.408125460959253 38.13624287954932 33.816137528324575 14.639494131166849 6 31.293963839831072 36.52876957468826 17.33592709268354 14.841339492797125 7 28.25511081126923 33.4708587992476 20.464113309739844 17.809917079743332 8 26.443838725722813 34.58866712024104 19.56358712534852 19.403907028687627 9 26.75636195235182 16.90043375431896 20.403462030298005 35.939742263031214 10-11 25.051479738756406 26.80293432128693 26.90726357435666 21.238322365600006 12-13 26.449925293632237 24.303606106864407 27.44402501519855 21.8024435843048 14-15 23.078168248584422 25.39491961532753 26.564759765522172 24.96215237056587 16-17 21.95253034361197 28.823149789490408 26.97734053002248 22.24697933687514 18-19 21.82463352211243 27.273642262123627 29.616056264246428 21.285667951517517 20-21 22.871595095298265 26.023770251238275 29.518067696928462 21.586566956535 22-23 22.40653842708792 26.167560951438958 29.351574455758396 22.074326165714734 24-25 22.466224908094016 26.34964581177426 29.492708982487837 21.69142029764389 26-27 22.155786051310418 26.362383207153044 29.701613728357966 21.780217013178564 28-29 21.744710522353785 26.833629204853626 29.663799905092418 21.757860367700168 30-31 21.92148303574662 26.527454590153628 29.520985914419285 22.03007645968048 32-33 21.82359768334029 26.638644632099723 29.4143697317241 22.123387952835888 34-35 22.481654563437395 26.45446318565857 29.220046011449615 21.84383623945442 36-37 22.37577133440957 26.22950975910695 29.339817444651615 22.054901461831868 38-39 22.10814159186615 26.652601985812225 29.48097277400218 21.758283648319445 40-41 22.183133321198873 26.527750909369868 29.418782335400955 21.870333434030304 42-43 21.856847161279724 26.77802303671058 28.895040016275953 22.470089785733744 44-45 22.241239331612068 26.68101109135947 28.715848413481538 22.36190116354693 46-47 22.40287805260953 26.96366188194272 28.29146059645289 22.341999468994864 48-49 22.494271656129516 27.014973319740392 28.100717369582135 22.39003765454796 50-51 22.14921744321626 27.26049560162816 28.20289410044175 22.387392854713834 52-53 22.569994339849178 27.30339075432468 27.808027980583965 22.318586925242176 54-55 21.99733286832431 26.998859870292975 28.110676912111387 22.893130349271328 56-57 22.340122179215243 27.427671086158167 27.72135096556075 22.510855769065845 58-59 22.60457543003718 26.904953192158416 27.786437845535016 22.704033532269392 60-61 22.365841814185515 26.929749468791602 28.35421817999469 22.350190537028194 62-63 22.20034551695091 26.98653189028074 28.256992763773507 22.556129828994838 64-65 22.080901088387925 27.165507955656437 28.039710627171395 22.71388032878425 66-67 22.072548253318203 27.318674331627683 27.630689209240312 22.978088205813805 68-69 21.99477813630446 27.100817464656537 27.95825605951781 22.946148339521194 70-71 22.112890912965337 27.09539378746332 27.77692347182897 23.014791827742382 72-73 22.166494194057414 26.863859555840154 27.901065518627995 23.06858073147444 74-75 21.90126991296327 27.46436915999169 27.58704149443228 23.04731943261276 76-77 22.089330937808764 26.95337132645794 27.845886647525838 23.111411088207454 78-79 21.989763734680693 27.06585893094727 27.867072965264043 23.077304369107992 80-81 22.016559992900987 27.222124617430577 27.677890688324208 23.083424701344228 82-83 21.981467294047693 27.106071607578127 27.71260822418009 23.199852874194097 84-85 21.740613106774838 27.09369598319869 27.635495815871675 23.5301950941548 86-87 21.923997273900582 26.96296276855843 27.739755564841907 23.373284392699084 88-89 21.822251312560777 26.926625782179624 27.510124222570724 23.740998682688875 90-91 21.739068631257055 26.882261946712376 27.810238247397436 23.568431174633133 92-93 21.98114569456911 26.934242197281982 27.690274927056475 23.39433718109243 94-95 21.73704495747382 26.689475618823057 27.67937096079892 23.894108462904207 96-97 21.620620767989084 26.501250188195254 27.840068817523978 24.038060226291684 98-99 21.499869692345293 26.679920567121783 27.483456645190124 24.3367530953428 100-101 21.55570338616127 26.192964202825443 28.128490339164042 24.12284207184925 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3053.0 1 2009.5 2 1135.0 3 1391.5 4 1263.0 5 1243.5 6 2218.0 7 4576.5 8 7059.5 9 7799.0 10 6470.0 11 5114.5 12 5016.0 13 5306.5 14 5675.0 15 5939.5 16 6104.5 17 6338.0 18 6786.5 19 7380.0 20 8164.0 21 9243.0 22 10679.5 23 12628.5 24 14658.0 25 17287.0 26 22224.5 27 28229.5 28 33225.5 29 38565.0 30 44944.0 31 53035.0 32 62241.0 33 69597.5 34 77933.0 35 85298.5 36 93272.5 37 103548.0 38 110299.0 39 113136.0 40 114541.0 41 120517.5 42 127730.0 43 134178.0 44 140582.5 45 144769.5 46 147679.0 47 159639.0 48 192830.0 49 196814.5 50 171539.0 51 159846.5 52 149993.5 53 146290.0 54 143688.0 55 132322.0 56 118725.0 57 104715.5 58 94079.5 59 84741.5 60 72825.5 61 59364.0 62 46695.5 63 35628.0 64 26064.0 65 19631.5 66 14194.0 67 10819.0 68 7937.0 69 5177.5 70 3735.0 71 2819.0 72 2230.0 73 1929.0 74 1593.5 75 1224.0 76 848.0 77 503.0 78 302.0 79 175.5 80 103.0 81 65.0 82 47.5 83 31.0 84 13.5 85 8.0 86 6.5 87 5.0 88 3.0 89 3.5 90 3.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38944505746863567 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.573327539778282E-4 16-17 0.0023107518090566223 18-19 1.0719982619334848E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.0719982619334848E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00796852041370557 36-37 0.01761650477110693 38-39 0.011744336514071288 40-41 0.00848069736107379 42-43 0.0038949270183583273 44-45 0.0020487077894728815 46-47 0.0016318195764987488 48-49 3.573327539778282E-5 50-51 5.121769473682204E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.883547637696986E-4 60-61 6.551100489593517E-4 62-63 0.0 64-65 0.0 66-67 0.0012268424553238768 68-69 7.86132058751222E-4 70-71 0.0018700414124839676 72-73 0.0 74-75 0.0 76-77 0.09801637441611828 78-79 0.03599531941736656 80-81 1.191109179926094E-5 82-83 0.0 84-85 0.0 86-87 0.15321237381389347 88-89 0.3303303088689037 90-91 0.2856994478970729 92-93 0.0 94-95 2.382218359852188E-5 96-97 0.0 98-99 2.3822183598521882E-4 100-101 4.883547637696986E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4197768.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.231010489077484 #Duplication Level Percentage of deduplicated Percentage of total 1 64.24781667371968 23.277633198058652 2 15.554731834021862 11.27127304466467 3 7.017640039375421 7.627685696255429 4 3.7371871678291173 5.4160826990905 5 2.3291640889990393 4.2193984269653395 6 1.5158546381855071 3.2952567177609513 7 1.0804803281789062 2.7402825872444283 8 0.7961067682557854 2.307500213688075 9 0.5642354809693426 1.8398539466378938 >10 2.8178851401003597 17.94984615027117 >50 0.19976734976628463 5.037763847533901 >100 0.12570611743836324 8.635277913059056 >500 0.009015387917112018 2.187535464510532 >1k 0.004079956493796815 2.6568669349347975 >5k 6.58057498999486E-5 0.1365197967392642 >10k+ 2.632229995997944E-4 1.401223362585277 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19783 0.47127425812955837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16140 0.38449004328014313 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11828 0.2817687876033168 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11020 0.2625204632557111 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5726 0.1364058232851363 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4399 0.10479378564989775 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4359 0.10384089830595689 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.382218359852188E-5 0.0 0.0 0.0 0.0 3 2.382218359852188E-5 0.0 0.0 0.0 0.0 4 2.382218359852188E-5 0.0 0.0 0.0 0.0 5 9.528873439408752E-5 0.0 0.0 0.0 0.0 6 1.429331015911313E-4 0.0 0.0 0.0 0.0 7 1.6675528518965315E-4 0.0 0.0 0.0 0.0 8 1.6675528518965315E-4 0.0 0.0 0.0 0.0 9 1.6675528518965315E-4 0.0 0.0 2.382218359852188E-5 0.0 10-11 1.6675528518965315E-4 0.0 0.0 2.382218359852188E-5 0.0 12-13 1.786663769889141E-4 0.0 0.0 4.764436719704376E-5 0.0 14-15 1.9057746878817504E-4 0.0 0.0 9.528873439408752E-5 0.0 16-17 2.1439965238669693E-4 0.0 0.0 1.786663769889141E-4 0.0 18-19 3.215994785800454E-4 0.0 0.0 1.9057746878817504E-4 0.0 20-21 3.811549375763501E-4 0.0 0.0 2.977772949815235E-4 0.0 22-23 4.2879930477339386E-4 0.0 0.0 6.670211407586127E-4 0.0 24-25 4.526214883719157E-4 0.0 0.0 0.0013102200979187035 0.0 26-27 4.526214883719157E-4 0.0 0.0 0.002417951635249971 0.0 28-29 5.479102227660033E-4 0.0 0.0 0.005764968430842295 0.0 30-31 6.431989571600908E-4 0.0 0.0 0.01656832869277197 0.0 32-33 6.551100489593517E-4 0.0 0.0 0.036900562394110395 0.0 34-35 7.146655079556564E-4 0.0 0.0 0.06827437819336371 0.0 36-37 7.623098751527002E-4 0.0 0.0 0.11918238454340498 0.0 38-39 8.337764259482659E-4 0.0 0.0 0.19336466426920212 0.0 40-41 8.337764259482659E-4 0.0 0.0 0.2756107531430989 0.0 42-43 9.290651603423534E-4 0.0 0.0 0.3622877681663208 0.0 44-45 0.001024353894736441 0.0 0.0 0.4643539137941878 0.0 46-47 0.0011553759045283113 0.0 0.0 0.5933986823473807 0.0 48-49 0.0012625757307216598 0.0 0.0 0.7309598815370454 0.0 50-51 0.0012625757307216598 0.0 0.0 0.8777164435957394 0.0 52-53 0.0012744868225209208 0.0 0.0 1.020613811911473 0.0 54-55 0.0012863979143201815 0.0 0.0 1.1804011083985584 0.0 56-57 0.0013340422815172252 0.0 0.0 1.3742660385233294 0.0 58-59 0.0013340422815172252 0.0 0.0 1.5944306593408688 0.0 60-61 0.0013697755569150082 0.0 0.0 1.8360233343052785 0.0 62-63 0.001405508832312791 0.0 0.0 2.074316636841293 0.0 64-65 0.001405508832312791 0.0 0.0 2.3168264658742457 0.0 66-67 0.001429331015911313 0.0 0.0 2.5967371231568777 0.0 68-69 0.001429331015911313 0.0 0.0 2.9210880639425523 0.0 70-71 0.0014531531995098349 0.0 0.0 3.277992018615607 0.0 72-73 0.0014769753831083566 0.0 0.0 3.632537577112408 0.0 74-75 0.0014769753831083566 0.0 0.0 3.9980770733399273 0.0 76-77 0.0014769753831083566 0.0 0.0 4.386783643117009 0.0 78-79 0.0015007975667068786 0.0 0.0 4.8270175960176935 0.0 80-81 0.0017032861272943144 0.0 0.0 5.314216983882863 0.0 82-83 0.0017151972190935754 0.0 0.0 5.809754136007516 0.0 84-85 0.0017390194026920972 0.0 0.0 6.30737572919704 0.0 86-87 0.0017747526780898801 0.0 0.0 6.8214941845285395 0.0 88-89 0.0017985748616884019 0.0 0.0 7.373501822873489 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 23485 0.0 24.338428 1 TACATGG 23885 0.0 23.581078 2 ACATGGG 23900 0.0 22.474327 3 GAGTACT 17820 0.0 21.316586 12-13 GTATCAA 39585 0.0 21.262178 1 AGTACTT 18845 0.0 20.308239 12-13 ATACCGT 285 1.9936124E-9 19.979036 6 CATGGGA 14845 0.0 19.817535 4 GTACTTT 19085 0.0 19.742054 14-15 TACCTGG 2885 0.0 19.407713 2 CATGGGG 8620 0.0 18.936047 4 GGTATCA 30570 0.0 18.557446 1 ATCAACG 45800 0.0 18.213423 3 TCAACGC 45915 0.0 18.178139 4 TATCAAC 46110 0.0 18.132133 2 CAACGCA 46185 0.0 18.061594 5 AGAGTAC 35825 0.0 17.769224 10-11 AACGCAG 47045 0.0 17.751595 6 TACTTTT 21800 0.0 17.370424 14-15 ACTTTTT 21995 0.0 17.172457 16-17 >>END_MODULE