FastQCFastQC Report
Thu 26 May 2016
SRR938734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204027
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205090.48784177646813404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148880.3541366408921731No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107970.2568251821408378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103840.2470012680698768No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60390.14364798323131608No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47010.11182135604742786No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA43210.10278240363346858No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG232150.025.4849281
TACATGG236750.024.4665182
GTATCAA360700.023.424391
ACATGGG239700.023.19593
GAGTACT170900.022.23344412-13
AGTACTT180600.020.68447312-13
GTACTTT185900.020.36322414-15
TATCAAC418850.020.104182
GGTATCA273000.020.0178721
ATCAACG420050.020.0131033
TCAACGC421850.019.8827274
CAACGCA423200.019.7968795
CATGGGA142850.019.7932764
CATGGGG92450.019.6536664
AACGCAG429300.019.5583866
TACCTGG28900.018.385132
ACTTTTT217000.017.90459816-17
ACGCAGA464950.017.8965727
AGAGTAC342650.017.88254210-11
CGCAGAG468550.017.7289058
Produced by FastQC (version 0.11.5)