FastQCFastQC Report
Thu 26 May 2016
SRR938734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204027
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197330.469383284170154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159090.378422878825469No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120090.2856546829979922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110260.26227234030609226No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT55860.13287260048520144No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44720.10637419788217346No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42610.10135520062073816No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42600.10133141390385933No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG233400.023.6808321
TACATGG236750.022.9722482
GAGTACT176400.022.25795712-13
ACATGGG238400.021.9771673
GTATCAA396850.021.0829371
GTACTTT186600.020.9904314-15
AGTACTT185400.020.44809712-13
CATGGGG87250.019.091964
GGTATCA308000.018.6709861
CATGGGA145550.018.5527764
ATCAACG455000.018.2634853
TATCAAC456250.018.2446522
TCAACGC455600.018.2290154
CAACGCA456450.018.1742745
ACTTTTT218950.018.07263616-17
AACGCAG463650.017.9182786
TACTTTT213650.017.72212814-15
AGAGTAC357750.017.50693710-11
TAGATCG1650.001473404817.2574335
AGTACAT185450.016.8642652
Produced by FastQC (version 0.11.5)