##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938734_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4204027 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07629446718587 33.0 31.0 34.0 30.0 34.0 2 32.275721825763725 34.0 31.0 34.0 30.0 34.0 3 32.353301964996895 34.0 31.0 34.0 30.0 34.0 4 35.97054800076213 37.0 35.0 37.0 35.0 37.0 5 35.87773223150089 37.0 35.0 37.0 35.0 37.0 6 35.90929316105724 37.0 35.0 37.0 35.0 37.0 7 35.829907610012974 37.0 35.0 37.0 35.0 37.0 8 35.8155240201835 37.0 35.0 37.0 33.0 37.0 9 37.45857911949662 39.0 37.0 39.0 34.0 39.0 10-11 37.388270103878966 39.0 37.0 39.0 34.0 39.0 12-13 37.309699366821384 39.0 37.0 39.0 34.0 39.0 14-15 38.5164242522705 40.0 38.0 41.0 34.0 41.0 16-17 38.4503773405832 40.0 38.0 41.0 33.5 41.0 18-19 38.61104626587793 40.0 38.0 41.0 34.0 41.0 20-21 38.61732417513018 40.0 38.0 41.0 34.0 41.0 22-23 38.543282785767076 40.0 38.0 41.0 34.0 41.0 24-25 38.46112144379663 40.0 38.0 41.0 34.0 41.0 26-27 38.28477695314517 40.0 38.0 41.0 33.5 41.0 28-29 38.11860164076016 40.0 38.0 41.0 33.0 41.0 30-31 37.89535759404019 40.0 37.0 41.0 33.0 41.0 32-33 37.74024274820309 40.0 37.0 41.0 33.0 41.0 34-35 37.701496802946316 40.0 37.0 41.0 33.0 41.0 36-37 37.583095446342284 40.0 37.0 41.0 32.5 41.0 38-39 37.45593689098571 40.0 37.0 41.0 32.0 41.0 40-41 37.280697531200445 40.0 36.0 41.0 31.5 41.0 42-43 37.076785662889414 40.0 36.0 41.0 31.0 41.0 44-45 36.81649689690385 40.0 36.0 41.0 30.5 41.0 46-47 36.48151700738364 39.0 35.5 41.0 30.0 41.0 48-49 36.16011112678392 39.0 35.0 41.0 29.5 41.0 50-51 35.92159541315981 39.0 35.0 41.0 28.5 41.0 52-53 35.69540776022609 39.0 35.0 40.5 28.0 41.0 54-55 35.41758842176799 38.5 35.0 40.0 27.5 41.0 56-57 35.1490926437913 38.0 34.0 40.0 27.0 41.0 58-59 34.845421901429276 38.0 34.0 40.0 26.0 41.0 60-61 34.477954113995935 37.5 34.0 40.0 26.0 41.0 62-63 34.117482356797424 37.0 33.5 40.0 25.5 41.0 64-65 33.708670401022644 36.5 33.0 39.0 24.5 41.0 66-67 33.297329322575706 36.0 33.0 39.0 23.5 40.0 68-69 32.961478363483394 35.5 32.0 39.0 23.0 40.0 70-71 32.52216838759599 35.0 32.0 38.0 22.0 40.0 72-73 32.03914758397127 35.0 31.5 37.0 20.5 39.5 74-75 31.550833760106677 35.0 31.0 37.0 19.5 39.0 76-77 29.615226543502217 33.0 28.5 35.0 17.5 37.0 78-79 30.5697659648713 34.0 30.5 36.0 17.5 37.5 80-81 30.620720918300478 34.0 31.0 35.5 17.5 37.0 82-83 30.305655149217642 34.0 30.5 35.0 13.0 37.0 84-85 29.917722816718353 34.0 30.0 35.0 7.5 36.0 86-87 29.35480921031192 34.0 29.5 35.0 3.5 36.0 88-89 28.760843353289594 34.0 29.0 35.0 2.0 35.5 90-91 28.25294128700886 33.5 28.5 35.0 2.0 35.0 92-93 27.964459909510573 33.0 27.0 35.0 2.0 35.0 94-95 27.62469020774605 33.0 27.0 35.0 2.0 35.0 96-97 27.019244167556487 33.0 26.5 35.0 2.0 35.0 98-99 26.195461399272652 33.0 25.0 35.0 2.0 35.0 100-101 22.49869244417317 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.135514326638706 1101 2 0.13876607334098168 1101 3 0.08072268985863218 1101 4 0.12408085291212956 1101 5 -0.016884510328551983 1101 6 0.0946100271868957 1101 7 0.2027393423477477 1101 8 0.21670336758576525 1101 9 0.27121487158467517 1101 10-11 0.276650002682743 1101 12-13 0.2137505802884334 1101 14-15 0.3532425622247146 1101 16-17 0.35357197186552014 1101 18-19 0.3306112604034368 1101 20-21 0.2835636549398046 1101 22-23 0.1505848593198209 1101 24-25 0.18918793314681892 1101 26-27 0.1666369748786778 1101 28-29 0.1734875425229916 1101 30-31 0.23428738204118105 1101 32-33 0.32578366055404473 1101 34-35 0.3614189817012061 1101 36-37 0.3787233633413507 1101 38-39 0.3504930495993932 1101 40-41 0.4177659971759624 1101 42-43 0.5133248689422132 1101 44-45 0.45411794959565555 1101 46-47 0.42070865279758607 1101 48-49 0.46283431100200545 1101 50-51 0.370530610067199 1101 52-53 0.30571164390262595 1101 54-55 0.44156848077178523 1101 56-57 0.41326736775636164 1101 58-59 0.4625538760257655 1101 60-61 0.548030798630073 1101 62-63 0.34681157100285986 1101 64-65 0.31725689877904273 1101 66-67 0.3199802330521706 1101 68-69 0.3292556733877916 1101 70-71 0.44888189775979725 1101 72-73 0.40016800901403116 1101 74-75 0.271050344787259 1101 76-77 0.1413380511284288 1101 78-79 0.026106768048688167 1101 80-81 0.2856314978443635 1101 82-83 0.41171569280992415 1101 84-85 0.43241194301072383 1101 86-87 0.516916347194222 1101 88-89 0.537817815025555 1101 90-91 0.5465688589476727 1101 92-93 0.5586353606060079 1101 94-95 0.611059039791261 1101 96-97 0.4113663597728312 1101 98-99 0.2373834438779845 1101 100-101 0.7301224986402843 1102 1 0.21396780579615893 1102 2 0.11806693318349204 1102 3 0.18868268422555445 1102 4 0.13035610470032566 1102 5 -0.024606849097359884 1102 6 0.018038741841735373 1102 7 0.058555838023515605 1102 8 0.063554806762248 1102 9 0.17750499299762623 1102 10-11 0.23711016119106176 1102 12-13 0.2164060885506558 1102 14-15 0.30468533865850134 1102 16-17 0.2674748184825191 1102 18-19 0.23285075978804315 1102 20-21 0.2548700947675684 1102 22-23 0.16907199597860512 1102 24-25 0.2275523578162506 1102 26-27 0.15355919276424856 1102 28-29 0.22005451497226147 1102 30-31 0.25484716351172665 1102 32-33 0.14548761055358028 1102 34-35 0.16610091091403945 1102 36-37 0.17918815069573668 1102 38-39 0.2338634532439272 1102 40-41 0.22471633697508508 1102 42-43 0.17697542524353338 1102 44-45 0.1477699999066644 1102 46-47 0.1355299806315884 1102 48-49 0.11145303914169347 1102 50-51 -0.032503903318463756 1102 52-53 -0.1532239669811375 1102 54-55 -0.13630386737569467 1102 56-57 -0.16150461664908988 1102 58-59 -0.049033508095988054 1102 60-61 0.024516795049379425 1102 62-63 -0.19292004284120878 1102 64-65 -0.13937958740413592 1102 66-67 -0.022675029732035057 1102 68-69 -0.07256334213734306 1102 70-71 -0.07558585835158027 1102 72-73 -0.1439193712904796 1102 74-75 -0.11950924640846594 1102 76-77 -0.09097860586985007 1102 78-79 -0.16740310468916775 1102 80-81 0.0020290235200128848 1102 82-83 -0.031157845729712363 1102 84-85 0.15972824823549558 1102 86-87 0.215752564150268 1102 88-89 0.17682508082221204 1102 90-91 0.16655410442744056 1102 92-93 0.17565830904117874 1102 94-95 0.15594384189546417 1102 96-97 0.25509039151771873 1102 98-99 0.371634808189544 1102 100-101 1.0148912871334979 1103 1 0.18140339568736152 1103 2 0.14133391296654452 1103 3 0.126333521930448 1103 4 0.054000773305368455 1103 5 -0.017923269473158143 1103 6 0.07818787286169027 1103 7 0.11825313272267834 1103 8 0.05774421845750055 1103 9 0.053131238371122436 1103 10-11 0.11206246541657805 1103 12-13 0.09350410588518798 1103 14-15 0.0856443354875509 1103 16-17 0.10294095975143591 1103 18-19 -0.09766194897448344 1103 20-21 0.07391453867121811 1103 22-23 0.06268742243094039 1103 24-25 0.12152318110150162 1103 26-27 0.15018040488722306 1103 28-29 0.037026281286074436 1103 30-31 0.1622325652804335 1103 32-33 0.18896368442023714 1103 34-35 0.16973315445689963 1103 36-37 0.10521408578616587 1103 38-39 0.11325316739763025 1103 40-41 0.2239231054494013 1103 42-43 0.2778348282886398 1103 44-45 0.32107432124571034 1103 46-47 0.3191791763326606 1103 48-49 0.18714514165294105 1103 50-51 0.22020775501340495 1103 52-53 0.19302986105231668 1103 54-55 0.18552559058257145 1103 56-57 0.1647033920026928 1103 58-59 0.23560509329215762 1103 60-61 0.28048573856512604 1103 62-63 0.18150514811945584 1103 64-65 0.18741643140614173 1103 66-67 0.1573234088390052 1103 68-69 0.04572497309944623 1103 70-71 0.04577678940344043 1103 72-73 0.1716359891673207 1103 74-75 0.18426044014253407 1103 76-77 0.19702032802697644 1103 78-79 0.1405592398389075 1103 80-81 0.3432031424100792 1103 82-83 0.417696623153315 1103 84-85 0.4005373874736229 1103 86-87 0.37611731442002494 1103 88-89 0.3195543585000493 1103 90-91 0.3665947819222666 1103 92-93 0.3621753641884986 1103 94-95 0.46080070683256125 1103 96-97 0.5652026991292338 1103 98-99 0.6052339240551454 1103 100-101 1.2997208753854963 1104 1 0.10053758833829818 1104 2 0.07354706239619446 1104 3 0.029349401962477373 1104 4 -0.0046752820179989385 1104 5 -0.15938143304909147 1104 6 0.007592163434928523 1104 7 -0.03435410803014349 1104 8 -0.010192679570110386 1104 9 0.02995478653840422 1104 10-11 -0.01742314962857705 1104 12-13 -0.04924159100258407 1104 14-15 -0.0022204670378371816 1104 16-17 0.10250456652187268 1104 18-19 0.1340798434977799 1104 20-21 0.09591001304446678 1104 22-23 0.08887536252422024 1104 24-25 0.14295667348930863 1104 26-27 0.19356610730192614 1104 28-29 0.13334453370418942 1104 30-31 -0.07475199670541599 1104 32-33 0.09158521405435494 1104 34-35 0.09918184566923571 1104 36-37 0.24071727996189196 1104 38-39 0.19631346852202824 1104 40-41 0.2978221315215279 1104 42-43 0.3099061229317357 1104 44-45 0.40429699860368373 1104 46-47 0.27503943005092424 1104 48-49 0.29849228601859323 1104 50-51 0.2064696064047169 1104 52-53 0.30764864790300095 1104 54-55 0.19989063189987633 1104 56-57 0.10760411330996078 1104 58-59 0.34347682947589675 1104 60-61 0.4080790000764267 1104 62-63 0.32230125072624105 1104 64-65 0.21841694057891203 1104 66-67 0.17284337810455952 1104 68-69 0.2297926943291131 1104 70-71 0.2847111161141953 1104 72-73 0.4374799723097311 1104 74-75 0.38373381958738406 1104 76-77 0.40513637851428186 1104 78-79 0.1694337452816903 1104 80-81 0.267654815822727 1104 82-83 0.2362305577976187 1104 84-85 0.2868319811404483 1104 86-87 0.44444009746458235 1104 88-89 0.41754057504176956 1104 90-91 0.41662043941250104 1104 92-93 0.5660205447562099 1104 94-95 0.5154517007648671 1104 96-97 0.5903815260410603 1104 98-99 0.8410023119136731 1104 100-101 1.5341663344131078 1105 1 0.10843586817168926 1105 2 0.1508122563029346 1105 3 0.09312189945106297 1105 4 0.09342340547389938 1105 5 0.00934915722677232 1105 6 -0.0014407823297091227 1105 7 0.07691149660087149 1105 8 0.09887415011716172 1105 9 0.14088996343456017 1105 10-11 0.12895608788853252 1105 12-13 0.1897661529374517 1105 14-15 0.17651373851920482 1105 16-17 0.1410809946340308 1105 18-19 0.12612608655767588 1105 20-21 0.10359795025179608 1105 22-23 0.02317383887661606 1105 24-25 0.0985039054805057 1105 26-27 0.13167676014047203 1105 28-29 0.12205730821972338 1105 30-31 0.12206694322862433 1105 32-33 0.18161694917294113 1105 34-35 0.1749180019728982 1105 36-37 0.15819260731417728 1105 38-39 0.25230428494415946 1105 40-41 0.26313358331894676 1105 42-43 0.3507270081338518 1105 44-45 0.41320891850060093 1105 46-47 0.34381657129154064 1105 48-49 0.38452286278626246 1105 50-51 0.3695839212856953 1105 52-53 0.3519301977889242 1105 54-55 0.42969401826673703 1105 56-57 0.48128866256774216 1105 58-59 0.46168639702727887 1105 60-61 0.45612416668676303 1105 62-63 0.3969078876033194 1105 64-65 0.31369957693071626 1105 66-67 0.31849986374911055 1105 68-69 0.38831480523495543 1105 70-71 0.4924510219508633 1105 72-73 0.47483524000904964 1105 74-75 0.5496844119251669 1105 76-77 0.7008245981706409 1105 78-79 0.4442109140810153 1105 80-81 0.5200500571984996 1105 82-83 0.6185617606071467 1105 84-85 0.5265508558134862 1105 86-87 0.5715077126302113 1105 88-89 0.5355709003501161 1105 90-91 0.4848625378538465 1105 92-93 0.6658656681691149 1105 94-95 0.8817629966730074 1105 96-97 0.8620367587054041 1105 98-99 1.0223450482615029 1105 100-101 1.7031850182725705 1106 1 0.1478415579876753 1106 2 0.14518559697500422 1106 3 0.11557233987525706 1106 4 0.09962521833145388 1106 5 0.08554705737603285 1106 6 0.128323396617823 1106 7 0.07614246380928336 1106 8 0.07654112876661401 1106 9 0.10639447666603274 1106 10-11 0.13278258259200726 1106 12-13 0.18746402582581823 1106 14-15 0.21895241217971773 1106 16-17 0.2578192213725856 1106 18-19 0.11765694543037597 1106 20-21 0.14069683888810403 1106 22-23 0.15618741372111344 1106 24-25 0.11845277387782716 1106 26-27 0.15803355969097765 1106 28-29 0.2645262255321086 1106 30-31 0.2585992537641957 1106 32-33 0.19815293736251505 1106 34-35 0.17094933995107908 1106 36-37 0.2285793710451145 1106 38-39 0.3138454964723252 1106 40-41 0.3886112365996013 1106 42-43 0.38081468662584683 1106 44-45 0.3877158218829706 1106 46-47 0.4534636964451977 1106 48-49 0.400642129440655 1106 50-51 0.43707308703911707 1106 52-53 0.41167110960396514 1106 54-55 0.4261604768572269 1106 56-57 0.3437185738366395 1106 58-59 0.4336154032831274 1106 60-61 0.43254476321348534 1106 62-63 0.4698002760212887 1106 64-65 0.44841607957361873 1106 66-67 0.38004828901235044 1106 68-69 0.31079554875004334 1106 70-71 0.3199098262550919 1106 72-73 0.3341401180123569 1106 74-75 0.4184565472172288 1106 76-77 0.4941381195794676 1106 78-79 0.39735470575958587 1106 80-81 0.33605417967248385 1106 82-83 0.45673666293668447 1106 84-85 0.5175078309900805 1106 86-87 0.642037292105119 1106 88-89 0.7883401235626941 1106 90-91 0.6487893991177245 1106 92-93 0.8103116892484614 1106 94-95 0.9619280188332198 1106 96-97 1.1300366893238625 1106 98-99 1.294495659378338 1106 100-101 1.7168142494726695 1107 1 0.030399104022244217 1107 2 0.02687839482304355 1107 3 -0.008886886952822692 1107 4 -0.008334764015415885 1107 5 -0.031050257084785926 1107 6 -0.03407193407144149 1107 7 0.03175531615777061 1107 8 -0.019351915173039913 1107 9 0.0046541131508277545 1107 10-11 -0.06365442956564493 1107 12-13 0.013309357963152024 1107 14-15 0.03468101951958147 1107 16-17 0.05006760119860587 1107 18-19 0.0505856349247793 1107 20-21 0.024044046205276004 1107 22-23 0.04227978336655269 1107 24-25 -0.04676931570089948 1107 26-27 -0.012504448869350426 1107 28-29 -0.006642475823156246 1107 30-31 0.03444797358030627 1107 32-33 -0.02586509412243032 1107 34-35 0.027124042147548266 1107 36-37 0.056739670159885236 1107 38-39 0.04926281465826321 1107 40-41 -0.03684678421482346 1107 42-43 0.017237467875581558 1107 44-45 0.031158351838683984 1107 46-47 0.0035184971314521363 1107 48-49 0.0921872537206383 1107 50-51 -0.014586224600492415 1107 52-53 -0.023280595381557134 1107 54-55 0.024412992574063708 1107 56-57 -0.050228959388519456 1107 58-59 0.06869233281861398 1107 60-61 0.13485194517221544 1107 62-63 0.0738658625453894 1107 64-65 0.03175805259494524 1107 66-67 0.08999610813103232 1107 68-69 -0.003828303325036586 1107 70-71 -0.07741869010899194 1107 72-73 0.01472555673270648 1107 74-75 0.13967271593054065 1107 76-77 0.06324490692012574 1107 78-79 0.2177664668238073 1107 80-81 0.21242019139677382 1107 82-83 0.18044722603798036 1107 84-85 0.20408784039056016 1107 86-87 0.18199293078354017 1107 88-89 0.15210410305169475 1107 90-91 0.3236056075798004 1107 92-93 0.33236656751919824 1107 94-95 0.4588076345455683 1107 96-97 0.6230460613704345 1107 98-99 0.8218778817434504 1107 100-101 1.5231565687014808 1108 1 0.11181495839905153 1108 2 0.028714089786220143 1108 3 0.014860509096180863 1108 4 0.03236204371584961 1108 5 -0.01757550201688929 1108 6 0.038362815853709265 1108 7 -0.0031265367144399647 1108 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5 125.0 6 285.0 7 689.0 8 1599.0 9 2957.0 10 4838.0 11 7518.0 12 10269.0 13 14113.0 14 17560.0 15 23303.0 16 30134.0 17 40608.0 18 37949.0 19 26456.0 20 24258.0 21 25290.0 22 26997.0 23 29986.0 24 35009.0 25 41891.0 26 50056.0 27 60847.0 28 74049.0 29 89994.0 30 109661.0 31 132250.0 32 162716.0 33 205730.0 34 272972.0 35 384754.0 36 578215.0 37 893651.0 38 723543.0 39 63687.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24969313860789 19.651169888194683 14.660344733857947 22.438792239339485 2 15.71899990176086 21.31917801669685 39.197131702531884 23.76469037901041 3 19.667904130967763 25.304000188390795 30.029778590860616 24.998317089780823 4 13.01131034600872 17.109880597817284 36.95028599959039 32.9285230565836 5 13.394042772292494 38.147823589692834 33.82971742344084 14.628416214573834 6 31.29084199661596 36.60389647306887 17.317993754776868 14.787267775538298 7 28.282216075205984 33.41836291726956 20.485168149490953 17.8142528580335 8 26.511437723877606 34.51628640824619 19.53415142195804 19.438124445918163 9 26.74576067185106 16.93994829243485 20.391377124837685 35.9229139108764 10-11 25.06520533764412 26.790420232791085 26.90962736442939 21.234747065135405 12-13 26.493562006143158 24.29891625339228 27.3840058591441 21.823515881320457 14-15 23.08209485809677 25.370626782368426 26.555835630931963 24.991442728602838 16-17 21.93598862392121 28.856583241930306 26.94427971680643 22.263148417342048 18-19 21.786384816272587 27.292415105802124 29.616662785467362 21.304537292457923 20-21 22.86866854090138 26.009799651619748 29.482244048385038 21.63928775909384 22-23 22.41232037758083 26.176556430298852 29.344911438485056 22.066211753635265 24-25 22.472683851032883 26.326095411172417 29.48419546552522 21.717025272269485 26-27 22.16572281844991 26.35054540610648 29.71044683279109 21.773284942652523 28-29 21.69523411719287 26.82887146062573 29.661072585880156 21.814821836301242 30-31 21.90986166359065 26.544727234149544 29.48565744225715 22.05975366000266 32-33 21.815452184298533 26.628813278316244 29.42108839928954 22.134646138095686 34-35 22.497732669716733 26.44175146729722 29.219152265278538 21.841363597707513 36-37 22.411280093478965 26.217743041566045 29.324338968550535 22.046637896404455 38-39 22.14946854407555 26.626406908796174 29.446088727529485 21.77803581959879 40-41 22.21991339478594 26.515347411656425 29.39519796140185 21.869541232155782 42-43 21.891081086738907 26.788331030597835 28.862303553825015 22.45828432883824 44-45 22.241846982532536 26.67999247360708 28.710227449782614 22.36793309407777 46-47 22.418174186933346 26.98105961539308 28.255891136229216 22.344875061444363 48-49 22.506025282876685 27.0137361225818 28.046930602442654 22.433307992098865 50-51 22.19657088188452 27.267758009555365 28.13058579211758 22.405085316442538 52-53 22.584464847632997 27.305783240688037 27.76221465751766 22.347537254161306 54-55 22.035170088108377 27.00402495036307 28.084239230623396 22.876565730905156 56-57 22.36438148174446 27.408434478786187 27.689751866717426 22.537432172751927 58-59 22.62296536472686 26.901143646457466 27.733468220453382 22.742422768362296 60-61 22.3810064121045 26.931980692324597 28.31137051954317 22.37564237602773 62-63 22.279832678461222 26.96186002788058 28.220553679631177 22.537753614027018 64-65 22.09456097928515 27.1577221929443 27.98142087070431 22.76629595706624 66-67 22.084100943588215 27.295691379702642 27.633160382402227 22.98704729430692 68-69 22.018144799452998 27.084837599870315 27.92439226839013 22.972625332286555 70-71 22.145523211276057 27.085302924686317 27.740175572282123 23.028998291755507 72-73 22.219600397428465 26.847662966960012 27.8804346404055 23.052301995206026 74-75 21.956880196231786 27.41592071185402 27.549138821810043 23.078060270104146 76-77 22.108833417985196 26.944545333809987 27.79665321281193 23.14996803539289 78-79 22.037099543908052 27.059387235571126 27.821358785743023 23.082154434777802 80-81 22.052079220167556 27.195907211737445 27.6459093525659 23.106104215529093 82-83 22.01094031187251 27.107677497598416 27.665302051489853 23.216080139039224 84-85 21.77834490596754 27.08844400856607 27.578509843062378 23.554701242404008 86-87 21.949253873858922 26.96801581772894 27.685216526416916 23.397513781995226 88-89 21.888141636524058 26.880698289987816 27.4587154474029 23.77244462608523 90-91 21.77023229145012 26.863340038713453 27.74571823051041 23.62070943932602 92-93 22.009315860821555 26.92891292201047 27.644288921085643 23.41748229608234 94-95 21.773131583291587 26.69729453802961 27.625954623367093 23.90361925531171 96-97 21.660875192330586 26.475178724576892 27.784828561298824 24.079117521793698 98-99 21.515953632077053 26.626708153872464 27.42669112258318 24.4306470914673 100-101 21.65922333214795 26.028268416752198 28.174184731867502 24.138323519232348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2969.0 1 1934.5 2 1106.5 3 1392.5 4 1253.0 5 1232.5 6 2113.5 7 4397.0 8 6819.0 9 7526.5 10 6355.0 11 5083.5 12 5036.5 13 5306.5 14 5611.0 15 5858.0 16 6054.0 17 6480.5 18 6875.0 19 7203.0 20 7832.0 21 8988.5 22 10556.0 23 12374.5 24 14302.5 25 17321.0 26 22292.0 27 28088.5 28 33249.0 29 38504.5 30 44821.0 31 52830.5 32 62219.0 33 69643.0 34 77773.0 35 85675.5 36 93925.5 37 103987.0 38 110567.5 39 113067.5 40 114882.0 41 120854.5 42 127480.0 43 133998.0 44 140521.5 45 144511.0 46 147134.5 47 159276.5 48 193015.5 49 197137.5 50 171915.5 51 160202.5 52 150336.5 53 146987.5 54 144955.0 55 133455.0 56 119462.0 57 105446.5 58 94318.0 59 84756.0 60 73050.0 61 59539.5 62 46814.0 63 35956.0 64 26344.0 65 19762.0 66 14511.0 67 11073.0 68 7905.0 69 5248.5 70 3838.5 71 2876.5 72 2227.0 73 1896.5 74 1639.5 75 1271.0 76 870.0 77 500.5 78 293.0 79 201.0 80 147.0 81 97.0 82 59.5 83 45.5 84 31.0 85 14.5 86 10.5 87 8.5 88 5.0 89 1.5 90 2.0 91 2.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.236701619661339 2 0.0 3 0.0 4 0.0 5 0.027735311880727694 6 0.004138888736918198 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0028068325917031457 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.854406025460826E-4 26-27 0.0011774424855025907 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.007005188120818444 36-37 0.017328623246235096 38-39 0.013653575488454283 40-41 0.008420497775109437 42-43 0.007873403286896112 44-45 0.003663154399341394 46-47 4.8762769601622444E-4 48-49 0.0017840037659130163 50-51 3.568007531826033E-5 52-53 0.0 54-55 0.0 56-57 0.0013439495036544722 58-59 4.2816090381912384E-4 60-61 0.002450031838520542 62-63 6.065612804104256E-4 64-65 0.0014866698049275137 66-67 0.005554198391209191 68-69 2.973339609855027E-4 70-71 8.325350907594076E-5 72-73 0.0 74-75 4.519476206979641E-4 76-77 0.05958572578149474 78-79 0.1420066997666761 80-81 0.0011179756933054903 82-83 7.968550154411472E-4 84-85 0.0 86-87 0.10550598271609579 88-89 0.3580138757434241 90-91 0.3947048865290351 92-93 3.33014036303763E-4 94-95 3.092273194249228E-4 96-97 5.9466792197100544E-5 98-99 0.0 100-101 9.990421089112892E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4204027.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.81927346058112 #Duplication Level Percentage of deduplicated Percentage of total 1 63.78667815102337 22.847924678335808 2 15.796873561640403 11.316650678532433 3 6.9901854514570205 7.511500926777441 4 3.856935879263946 5.526105639171286 5 2.342424884685107 4.195197875270303 6 1.5861867537992267 3.4089634256331576 7 1.0678219035841827 2.6774023340176094 8 0.7659437499151223 2.194843890690618 9 0.5867721972477815 1.8915978413055903 >10 2.8726884646199777 18.149972920082956 >50 0.20470619285248715 5.094645306057174 >100 0.12877834930493792 8.693988023847147 >500 0.00946968294860302 2.260104130349588 >1k 0.004201338124177373 2.696044101020979 >5k 6.668790673297414E-5 0.13343347286430976 >10k+ 2.6675162693189656E-4 1.40162475604352 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19733 0.469383284170154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15909 0.378422878825469 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12009 0.2856546829979922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11026 0.26227234030609226 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5586 0.13287260048520144 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4472 0.10637419788217346 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4261 0.10135520062073816 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4260 0.10133141390385933 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.7573433757680436E-5 0.0 0.0 0.0 0.0 3 4.7573433757680436E-5 0.0 0.0 0.0 0.0 4 4.7573433757680436E-5 0.0 0.0 0.0 0.0 5 4.7573433757680436E-5 0.0 0.0 0.0 0.0 6 7.136015063652065E-5 0.0 0.0 0.0 0.0 7 1.1893358439420109E-4 0.0 0.0 0.0 0.0 8 1.1893358439420109E-4 0.0 0.0 0.0 0.0 9 1.1893358439420109E-4 0.0 0.0 0.0 0.0 10-11 1.1893358439420109E-4 0.0 0.0 1.1893358439420109E-5 0.0 12-13 1.1893358439420109E-4 0.0 0.0 5.9466792197100544E-5 0.0 14-15 1.1893358439420109E-4 0.0 0.0 8.325350907594076E-5 0.0 16-17 1.427203012730413E-4 0.0 0.0 1.5461365971246142E-4 0.0 18-19 1.9029373503072174E-4 0.0 0.0 2.2597381034898206E-4 0.0 20-21 2.1408045190956195E-4 0.0 0.0 3.5680075318260325E-4 0.0 22-23 2.854406025460826E-4 0.0 0.0 9.395753167141885E-4 0.0 24-25 2.854406025460826E-4 0.0 0.0 0.0014866698049275135 0.0 26-27 2.854406025460826E-4 0.0 0.0 0.0024738185553993828 0.0 28-29 3.686941116220234E-4 0.0 0.0 0.006089399520983096 0.0 30-31 4.519476206979641E-4 0.0 0.0 0.015818166724428744 0.0 32-33 4.519476206979641E-4 0.0 0.0 0.03474050000154614 0.0 34-35 4.876276960162245E-4 0.0 0.0 0.06414088206379265 0.0 36-37 5.233077713344848E-4 0.0 0.0 0.1119165029149432 0.0 38-39 5.470944882133249E-4 0.0 0.0 0.18101691544797405 0.0 40-41 5.58987846652745E-4 0.0 0.0 0.2575149969303242 0.0 42-43 6.065612804104256E-4 0.0 0.0 0.3362252430824065 0.0 44-45 7.136015063652065E-4 0.0 0.0 0.43535638567497303 0.0 46-47 9.752553920324489E-4 0.0 0.0 0.5594160075565642 0.0 48-49 0.0011179756933054903 0.0 0.0 0.6930378896234491 0.0 50-51 0.0011179756933054903 0.0 0.0 0.8359365912730817 0.0 52-53 0.0011179756933054903 0.0 0.0 0.9784190253773346 0.0 54-55 0.0011179756933054903 0.0 0.0 1.137112107034517 0.0 56-57 0.0011893358439420108 0.0 0.0 1.3278458963274975 0.0 58-59 0.0011893358439420108 0.0 0.0 1.538881648476568 0.0 60-61 0.001201229202381431 0.0 0.0 1.7728596890552795 0.0 62-63 0.001213122560820851 0.0 0.0 2.0091093610959208 0.0 64-65 0.001213122560820851 1.1893358439420109E-5 0.0 2.2487843203671147 0.0 66-67 0.001308269428336212 2.3786716878840218E-5 0.0 2.5256498114783756 0.0 68-69 0.001308269428336212 2.3786716878840218E-5 0.0 2.839396607110278 0.0 70-71 0.001332056145215052 2.3786716878840218E-5 0.0 3.191107002880809 0.0 72-73 0.0013915229374121527 2.3786716878840218E-5 0.0 3.5457907382611955 0.0 74-75 0.001427203012730413 2.3786716878840218E-5 0.0 3.9065519798041257 0.0 76-77 0.001439096371169833 2.3786716878840218E-5 0.0 4.287472463901873 0.0 78-79 0.0014747764464880934 2.3786716878840218E-5 0.0 4.729608063887316 0.0 80-81 0.0015818166724428744 2.3786716878840218E-5 0.0 5.2107181994787375 0.0 82-83 0.0015937100308822945 2.3786716878840218E-5 0.0 5.696645145238126 0.0 84-85 0.0016888568983976554 2.3786716878840218E-5 0.0 6.189006397913239 0.0 86-87 0.0018077904827918565 2.3786716878840218E-5 0.0 6.698862780852739 0.0 88-89 0.0018315771996706967 2.3786716878840218E-5 0.0 7.243043396248407 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 23340 0.0 23.680832 1 TACATGG 23675 0.0 22.972248 2 GAGTACT 17640 0.0 22.257957 12-13 ACATGGG 23840 0.0 21.977167 3 GTATCAA 39685 0.0 21.082937 1 GTACTTT 18660 0.0 20.99043 14-15 AGTACTT 18540 0.0 20.448097 12-13 CATGGGG 8725 0.0 19.09196 4 GGTATCA 30800 0.0 18.670986 1 CATGGGA 14555 0.0 18.552776 4 ATCAACG 45500 0.0 18.263485 3 TATCAAC 45625 0.0 18.244652 2 TCAACGC 45560 0.0 18.229015 4 CAACGCA 45645 0.0 18.174274 5 ACTTTTT 21895 0.0 18.072636 16-17 AACGCAG 46365 0.0 17.918278 6 TACTTTT 21365 0.0 17.722128 14-15 AGAGTAC 35775 0.0 17.506937 10-11 TAGATCG 165 0.0014734048 17.257433 5 AGTACAT 18545 0.0 16.864265 2 >>END_MODULE