FastQCFastQC Report
Thu 26 May 2016
SRR938733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145278
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.9788130343204063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.6704387450267763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.48940651716020317No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5950.4095596029681025No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5690.3916628808215973No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5260.3620644557331461No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4030.27739919327083246No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT3150.21682567215958368No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.20925398202067758No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2990.2058123046848112No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.1982406145459051No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2750.18929225347265244No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.17208386679332038No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2370.16313550572006771No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2220.1528104737124685No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.14661545450790897No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT2080.14317377717204255No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1830.12596539049271055No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1750.12045870675532427No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1700.11701702941945788No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1700.11701702941945788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.10669199741185866No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1530.10531532647751207No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC1490.10256198460881895No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG205.355443E-447.54669656-57
CTTACTA1051.0459189E-940.585859
CGCATCC250.001603316338.05051888-89
CACGCAT250.001603316338.05051886-87
TGCACGC250.001606044538.03735784-85
ACTGTAG550.002612402734.4364748
ATATAGG1308.409188E-932.792172
GATTCTA452.368128E-531.74171632-33
CCGGGTT300.00392052231.70876360-61
ATAACTC300.003927169431.69779884-85
GATATAG1509.622454E-1031.5994261
GCCCGAC300.00400425931.57220626-27
AGGCTTA1401.736953E-830.4393866
CAATACT953.5374043E-529.9156674
CACCGGG404.4506486E-429.72696958-59
GCCGGAG404.459679E-429.71668638-39
GGGACAG804.548903E-429.6040445
ACTGGGC804.558098E-429.5938458
TACACTG650.005908192629.1485965
TATAGGC1503.385867E-828.4198843
Produced by FastQC (version 0.11.5)